RchiOBHm_Chr1g0340941

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000491
Physical Location & Seq
Forward (+)
32584464 .. 32607616
23153 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56770

Sequence Viewer

Length: 3612 bp
ATGCTTACATGGGAAGAAGCGCGAGCAATTTTATCTTTAATTTGGGTGACATTCACATGCTTCTGCGAGTGGGTTCCGCATGTTCTAGTTTGGATGGTTGCGGCTCCAATATTTGTTCCACTGGTTGCAATTCAGTGGGCATGTAAACCAATTGCAACTTGGCGAGCATGTAAATCAATTGCGATTGCAATTACCGCAATTCGGTGGGCATGTAAATCAATTGCGGTTGCAATTTGGTTGGCATGGAAATCAATTGCGGTTGCAATTCCGTGGGCATGTAAGTCAATTGCGGTTGCAATTCCGTGGGCATGGAAGGCTTTTCTTGACATCCCTTCAGCAAAGTGGGAACATGTATTTGTAATATCATGTATTGCGGTCTCACTGGATCCTCTGTTCTTTTACATCCCAACCATCGACGAGACAAATAAATGTCTTTCAATGGACAAAAAGATGAGAACTACAGCTCTCGTCTTCCGATCGCTTACGGATGCCCATTTAGTACTCCATATTAAAGATCAAATTTGTGATCGGGTCGACTCTGACTACCCCAACTGGTCCGACTCTGACTCCAACTCCGACTCCGACTCCGACACCTACTCCGACTCCCATTCTGGGGCACCATCGTCTTCATTGAAGAAGAGGATGCAGTGGTTGTCCTTCTCTATTATAGTTAACTGCCTTGCCATTCTTCCCATACCACAGGTGGCTACATCAGTTGGCTTTTACCCAATAGGAGCAGGCTCATCGGCCTCTGAAGTTTCTATTAAAAGAGCAGCGATCAACCTTTCTCTTGCCCTCCAGTATCTGCCAAGGGTTTGTCAAATCTACCTATCTAGTAAGAAGTTTAGAACAAGCGGAAGATGGATTAAACCTTCGCTGAATTTGTTTCTTTACATCCTTAGTAGCCACATATTTGCTGCTTTTTGGTACTTTTTTGCTATTGGACGAGAGATCTCATGCTGGTATCAAACCTGCAAAGACACTGCAGGATGTGTTGCTGATTTTGATTGTAGTAGCCCTGCCAATACTTCAAGAAATATAGCAGTCTTAAATCAATTTTGCCCTATAAATTCACCGAATAGTACAGTATTCGATTTTGGAATCTATCTTCCAATCCTCCAGTCTGGTAACACGGGGAGTGTTAGTTTTACAACAAAGTTCTTTTACTCTTTATGGTGGGGCTTAAAAAATCTAAGCAATTTTGGCACAAACCTCGAAACCAGTACCTACAAGTGGGAGAACTGTTTTGCAATTGTCATTTGTGTTAGTGGATTGCTACTATTTCTCTATTTCATTGGAAATATGCAGATATATATGGAGTTGGACACCACAAAATCTTTGAAGACCCAGGAATCAAGACGCCAAAAGATTAAAGCAAAGAAAGCAGAAATAAAGAAGTGGATGTCAAATAATGGTATCTCTGGAAATATGGAAACCGGAATCAAGGACTACATCAAACATTCCAATGTCTTGGAAAAGAACATAAAGGCAGATGTGGATGTGACATATTTTCTCGACGGCAATCGTTTTGGCAATTCTAGTATCAGAGAGCCTCTGTTGGAGCATCTCGTACGGAATGCGGTAAAACAGGTACCGTTGATTCAGAAGATGCAGGATGTAAGCGGGTTAAAGACCTTGTGCAGATATATGAAGCCAGTTGTCTATAACAAAGACCACGCTCTTGAAGTACAGGAGCTTGTTCAGATGATCTTCATCATACAAGGTGAAATTGACCAATATTGTACCGAAACCAGCAGCAATTTTAAGCGTCTAAGTAAAGGTGATTGTTATGGATTACAAATTCAAGATTGGGCAGCAAGGAACTACATGAGGTCTTATTATGACCCTCTTCCAACTTTCACCGGATGGGTCAGATGCCAAACAAGAGTAGAAGCCTTTGAACTCAAGGCCATGGACTTGATGCAAATTATTACCCAGCAGAACTTTGATTCTGAGCAGTTGAAAAAGTTGGCAGGCATTGAAGACAACACTGATCAGATACCAAACGAGGATGATCTACAATCAATTCCCCAGTGTGAACAGTTAGACCCTGACTGGGTGAAGCTCAGTTGTAGTGTTGTGTGGAACCCGGAAACTAAGAAGGCGCGAACAGGTTACGTTTGCAGGGATGCGCGAGGAGCTTTCTTGGGTGCTGAAAGTAGAGGTGGTTTAGAATTCAGAGACAGATTTTTGGCTGAAGCTGACAACATAAACAAAGCCATTGCATTTTTTACTAGGAATAAATTTAAAGGCGGGGTGCGGATTGTGGTGGAGTCTGATAACAAAGATCTCATTGAGTGCCTCTGTATGCCTAATTTGGAATCAAATGAAAGACTAACTGACGTCCTTCGACCCGTCCTGGAAGAAATTATCAAGTCGGCTTCAAAATTTAAATCTAAACGTTTTAAACATTGTAAGCGCCAAAGCAATGCAGCAGCTAATTGCATAGCAAGGCATGCCGTGGATTCTCCTAATCCTTCTTCTTGGATTCGCAATGCACCTGATTGGCTGCTAAATATCCTCAACCAAGATAGCTTGCATCTTCAGGAGAAATCACCGTCAGAGCAAAACCAAGAGCTGAGAGTAGACATTGAACCGGAGAAGCCACCGTCAAAGAAAAACCAAGAGCTGAGAGTAGACACTGAACCTCCGGGACCACCATCAAAGAAAAACCAGGAGATGAGAGTTGACATTGAACCTCCGGAACCACCGTCAAAGAAAAACCAGGAGCTGAGAGTAGACATTGAACCTCCGGAGCTACTGTCAGAACAAAACCAGGAGCTGACAGTAGACATTGAACATTCGGATTCCGGATTCATGAATTTCCTAGGCAAAAAAGGACACATGGATGCTGATGTTGATGTGGATGTTCTATTCGCTAATCCTCCAAGTGATATCAAACAATCCAAAAAACGCCATCTCGGCTTGAATGCCCTAAAGAAAGTACCCATGCTTCAAAAGATGCACGAATACGTGTTGGAAGTCATCTGCAACCATCTCGAGCCAAAGGTGTACGAGAGGAATAGCTATGTTGTTAGAGCGAGAGAACCACTTGGTTTGATGATCTTCATTCTCCGAGGCACAATTGGAATGACTGACACGACTAGCAGCAATGCCACAACAACAGGTTCCTCGGAGATCACCGAGTCACGTGATGACAAGATCCTTAGGGAGGGGGATTTTTATGGAGAACAACTTCTGAGCTGGGCATCTCCCAACAATATTTCTTCTTCTGATAATCCCGTCATCTCAGCTATAGATGTCAGATGTCAAACAAAAGTGGAAGCCTTGATTCTCAAGGCCGAGGACTTGAAAAGTGTAGCCTCCAAATGCGGATCGCAGTGGAATTTCGACAATTGTATGAATTCTCAGCAGTTGGTTGATGATGGCAGAGATCGTGTGGCTAATACAGCACCAACGTCCACTATTGAGGAGACTATCCGGCTTCATCAGGGTCATGACATAATTCTAGAGCAACTAGTTCTAATTAGAGAGACCATATTCCGGAGATTAGATGATCAGGGCCGGAAATTAGACGAGATGGCTGCATTTTTAGCCAGAGAGGGTTACACAGGGATTACAGGCGCATCTTCTACTCCTTCAGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1203

Amino Acids

136.03

Weight (kDa)

6.49

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 693 - 820 6e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2346
Acc36I ACCTGC 1 cut(s) 980
Acc65I GGTACC 1 cut(s) 1591
AccB1I GGYRCC 2 cut(s) 616, 1591
AccI GTMKAC 5 cut(s) 534, 2586, 2637, 2739, 2790
AccII CGCG 3 cut(s) 22, 2107, 2134
AccIII TCCGGA 4 cut(s) 2701, 2752, 2810, 3504
AclI AACGTT 1 cut(s) 2401
AclWI GGATC 4 cut(s) 380, 393, 3157, 3343
AcuI CTGAAG 5 cut(s) 318, 774, 2217, 2528, 3585
AcvI CACGTG 1 cut(s) 3152
AcyI GRCGYC 2 cut(s) 1360, 2343
AfiI CCNNNNNNNGG 7 cut(s) 201, 612, 613, 1233, 2056, 3172, 3504
AflIII ACRYGT 2 cut(s) 349, 2973
AhlI ACTAGT 1 cut(s) 3478
AjnI CCWGG 5 cut(s) 1347, 2358, 2673, 2724, 2775
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
Alw26I GTCTC 5 cut(s) 382, 413, 2175, 3428, 3488
AlwI GGATC 4 cut(s) 380, 393, 3157, 3343
AlwNI CAGNNNCTG 3 cut(s) 805, 2731, 2782
Ama87I CYCGRG 1 cut(s) 2999
Aor13HI TCCGGA 4 cut(s) 2701, 2752, 2810, 3504
AoxI GGCC 4 cut(s) 747, 1908, 3300, 3523
ApeKI GCWGC 9 cut(s) 773, 917, 1755, 1814, 2432, 2435, 2509, 3108, 3545
Asp700I GAANNNNTTC 1 cut(s) 3195
Asp718I GGTACC 1 cut(s) 1591
AspA2I CCTAGG 1 cut(s) 2827
AspLEI GCGC 5 cut(s) 22, 2107, 2134, 2421, 3587
AspS9I GGNCC 3 cut(s) 555, 2654, 3523
AsuC2I CCSGG 2 cut(s) 2090, 2652
AsuHPI GGTGA 8 cut(s) 58, 1065, 1736, 1793, 1852, 2071, 2547, 3133
AvaI CYCGRG 1 cut(s) 2999
AvaII GGWCC 2 cut(s) 555, 2654
AvrII CCTAGG 1 cut(s) 2827
AxyI CCTNAGG 1 cut(s) 3167
BaeGI GKGCMC 1 cut(s) 619
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 2 cut(s) 616, 1591
BarI GAAGNNNNNNTAC 2 cut(s) 2937, 2969
BbrPI CACGTG 1 cut(s) 3152
BbsI GAAGAC 4 cut(s) 463, 618, 1349, 1989
BbvI GCAGC 9 cut(s) 785, 904, 1767, 1826, 2444, 2447, 2496, 3120, 3532
BceAI ACGGC 2 cut(s) 1534, 2444
BcgI CGANNNNNNTGC 8 cut(s) 726, 760, 1195, 1229, 2980, 3014, 3527, 3561
BciT130I CCWGG 5 cut(s) 1349, 2360, 2675, 2726, 2777
BclI TGATCA 2 cut(s) 1993, 3517
BcnI CCSGG 2 cut(s) 2090, 2652
BcoDI GTCTC 5 cut(s) 382, 413, 2175, 3428, 3488
BcuI ACTAGT 1 cut(s) 3478
BfaI CTAG 8 cut(s) 86, 834, 1539, 2235, 2828, 3105, 3470, 3479
BfmI CTRYAG 3 cut(s) 459, 984, 3255
BfoI RGCGCY 1 cut(s) 2422
BfuAI ACCTGC 1 cut(s) 980
BglI GCCNNNNNGGC 1 cut(s) 2922
BglII AGATCT 2 cut(s) 951, 2287
BlnI CCTAGG 1 cut(s) 2827
BmcAI AGTACT 1 cut(s) 501
Bme1390I CCNGG 7 cut(s) 1349, 2090, 2360, 2652, 2675, 2726, 2777
Bme18I GGWCC 2 cut(s) 555, 2654
BmeT110I CYCGRG 1 cut(s) 2999
BmgT120I GGNCC 3 cut(s) 555, 2654, 3523
BmiI GGNNCC 9 cut(s) 75, 105, 387, 618, 1593, 2087, 2655, 2706, 3130
BmrFI CCNGG 7 cut(s) 1349, 2090, 2360, 2652, 2675, 2726, 2777
BmrI ACTGGG 2 cut(s) 2026, 2065
BmuI ACTGGG 2 cut(s) 2026, 2065
BpiI GAAGAC 4 cut(s) 463, 618, 1349, 1989
BpmI CTGGAG 2 cut(s) 782, 1103
BpuEI CTTGAG 2 cut(s) 1889, 3281
BpuMI CCSGG 2 cut(s) 2090, 2652
BsaAI YACGTR 2 cut(s) 2974, 3152
BsaBI GATNNNNATC 1 cut(s) 1712
BsaHI GRCGYC 2 cut(s) 1360, 2343
BsaI GGTCTC 2 cut(s) 382, 3488
BsaWI WCCGGW 7 cut(s) 1436, 1862, 2596, 2701, 2752, 2810, 3504
Bsc4I CCNNNNNNNGG 7 cut(s) 201, 612, 613, 1233, 2056, 3172, 3504
Bse1I ACTGG 9 cut(s) 126, 387, 557, 799, 1120, 1221, 1655, 2032, 2060
Bse21I CCTNAGG 1 cut(s) 3167
Bse3DI GCAATG 4 cut(s) 2220, 2434, 2500, 3118
Bse8I GATNNNNATC 1 cut(s) 1712
BseAI TCCGGA 4 cut(s) 2701, 2752, 2810, 3504
BseBI CCWGG 5 cut(s) 1349, 2360, 2675, 2726, 2777
BseJI GATNNNNATC 1 cut(s) 1712
BseLI CCNNNNNNNGG 7 cut(s) 201, 612, 613, 1233, 2056, 3172, 3504
BseMI GCAATG 4 cut(s) 2220, 2434, 2500, 3118
BseMII CTCAG 8 cut(s) 1944, 2080, 2570, 2621, 2723, 3191, 3264, 3383
BseNI ACTGG 9 cut(s) 126, 387, 557, 799, 1120, 1221, 1655, 2032, 2060
BseRI GAGGAG 2 cut(s) 2151, 3446
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 9 cut(s) 785, 904, 1767, 1826, 2444, 2447, 2496, 3120, 3532
BseYI CCCAGC 2 cut(s) 1935, 3204
BsgI GTGCAG 1 cut(s) 1660
Bsh1236I CGCG 3 cut(s) 22, 2107, 2134
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 4 cut(s) 749, 1910, 3302, 3525
BshNI GGYRCC 2 cut(s) 616, 1591
BsiEI CGRYCG 1 cut(s) 479
BsiHKCI CYCGRG 1 cut(s) 2999
BsiWI CGTACG 1 cut(s) 1570
BslFI GGGAC 1 cut(s) 2667
BslI CCNNNNNNNGG 7 cut(s) 201, 612, 613, 1233, 2056, 3172, 3504
BsmAI GTCTC 5 cut(s) 382, 413, 2175, 3428, 3488
BsmFI GGGAC 1 cut(s) 2667
BsmI GAATGC 2 cut(s) 1582, 2935
BsnI GGCC 4 cut(s) 749, 1910, 3302, 3525
Bso31I GGTCTC 2 cut(s) 382, 3488
BsoBI CYCGRG 1 cut(s) 2999
Bsp1286I GDGCHC 1 cut(s) 619
Bsp13I TCCGGA 4 cut(s) 2701, 2752, 2810, 3504
Bsp19I CCATGG 1 cut(s) 1911
BspANI GGCC 4 cut(s) 749, 1910, 3302, 3525
BspCNI CTCAG 8 cut(s) 1945, 2079, 2571, 2622, 2724, 3192, 3263, 3382
BspEI TCCGGA 4 cut(s) 2701, 2752, 2810, 3504
BspFNI CGCG 3 cut(s) 22, 2107, 2134
BspHI TCATGA 2 cut(s) 2817, 3457
BspLI GGNNCC 9 cut(s) 75, 105, 387, 618, 1593, 2087, 2655, 2706, 3130
BspMAI CTGCAG 1 cut(s) 988
BspMI ACCTGC 1 cut(s) 980
BspPI GGATC 4 cut(s) 380, 393, 3157, 3343
BspT107I GGYRCC 2 cut(s) 616, 1591
BspTNI GGTCTC 2 cut(s) 382, 3488
BsrDI GCAATG 4 cut(s) 2220, 2434, 2500, 3118
BsrI ACTGG 9 cut(s) 126, 387, 557, 799, 1120, 1221, 1655, 2032, 2060
BssNI GRCGYC 2 cut(s) 1360, 2343
BssT1I CCWWGG 3 cut(s) 809, 1911, 2827
Bst2UI CCWGG 5 cut(s) 1349, 2360, 2675, 2726, 2777
Bst4CI ACNGT 9 cut(s) 1087, 1244, 1596, 2043, 2559, 2610, 2712, 2763, 2788
Bst6I CTCTTC 2 cut(s) 632, 1854
BstACI GRCGYC 2 cut(s) 1360, 2343
BstAPI GCANNNNNTGC 1 cut(s) 2456
BstBAI YACGTR 2 cut(s) 2974, 3152
BstC8I GCNNGC 6 cut(s) 24, 165, 739, 1975, 2457, 2537
BstDSI CCRYGG 4 cut(s) 269, 302, 1911, 2460
BstENI CCTNNNNNAGG 1 cut(s) 3170
BstFNI CGCG 3 cut(s) 22, 2107, 2134
BstH2I RGCGCY 1 cut(s) 2422
BstHHI GCGC 5 cut(s) 22, 2107, 2134, 2421, 3587
BstMAI GTCTC 5 cut(s) 382, 413, 2175, 3428, 3488
BstMCI CGRYCG 1 cut(s) 479
BstMWI GCNNNNNNNGC 9 cut(s) 194, 314, 1203, 1382, 2138, 2456, 2922, 3410, 3554
BstNI CCWGG 5 cut(s) 1349, 2360, 2675, 2726, 2777
BstNSI RCATGY 8 cut(s) 60, 83, 144, 171, 213, 279, 353, 2459
BstSCI CCNGG 7 cut(s) 1347, 2088, 2358, 2650, 2673, 2724, 2775
BstSFI CTRYAG 3 cut(s) 459, 984, 3255
BstSLI GKGCMC 1 cut(s) 619
BstUI CGCG 3 cut(s) 22, 2107, 2134
BstV1I GCAGC 9 cut(s) 785, 904, 1767, 1826, 2444, 2447, 2496, 3120, 3532
BstV2I GAAGAC 4 cut(s) 463, 618, 1349, 1989
BstX2I RGATCY 4 cut(s) 385, 951, 2287, 3162
BstXI CCANNNNNNTGG 1 cut(s) 1471
BstYI RGATCY 4 cut(s) 385, 951, 2287, 3162
Bsu36I CCTNAGG 1 cut(s) 3167
BsuRI GGCC 4 cut(s) 749, 1910, 3302, 3525
BtgI CCRYGG 4 cut(s) 269, 302, 1911, 2460
BtsI GCAGTG 3 cut(s) 653, 981, 3347
BtsIMutI CAGTG 9 cut(s) 119, 140, 380, 653, 981, 1989, 2039, 2640, 3347
BveI ACCTGC 1 cut(s) 980
Cac8I GCNNGC 6 cut(s) 24, 165, 739, 1975, 2457, 2537
CaiI CAGNNNCTG 3 cut(s) 805, 2731, 2782
CciI TCATGA 2 cut(s) 2817, 3457
CfoI GCGC 5 cut(s) 22, 2107, 2134, 2421, 3587
Cfr13I GGNCC 3 cut(s) 555, 2654, 3523
CseI GACGC 2 cut(s) 1368, 1757
CspCI CAANNNNNGTGG 2 cut(s) 3039, 3074
DraI TTTAAA 3 cut(s) 2248, 2392, 2407
Eam1104I CTCTTC 2 cut(s) 632, 1854
EarI CTCTTC 2 cut(s) 632, 1854
Eco130I CCWWGG 3 cut(s) 809, 1911, 2827
Eco31I GGTCTC 2 cut(s) 382, 3488
Eco32I GATATC 1 cut(s) 2896
Eco47I GGWCC 2 cut(s) 555, 2654
Eco57I CTGAAG 5 cut(s) 318, 774, 2217, 2528, 3585
Eco72I CACGTG 1 cut(s) 3152
Eco81I CCTNAGG 1 cut(s) 3167
Eco88I CYCGRG 1 cut(s) 2999
EcoNI CCTNNNNNAGG 1 cut(s) 3170
EcoRI GAATTC 2 cut(s) 2174, 3364
EcoRII CCWGG 5 cut(s) 1347, 2358, 2673, 2724, 2775
EcoRV GATATC 1 cut(s) 2896
EcoT14I CCWWGG 3 cut(s) 809, 1911, 2827
ErhI CCWWGG 3 cut(s) 809, 1911, 2827
FaqI GGGAC 1 cut(s) 2667
FauI CCCGC 2 cut(s) 1616, 2246
FbaI TGATCA 2 cut(s) 1993, 3517
FblI GTMKAC 5 cut(s) 534, 2586, 2637, 2739, 2790
FspBI CTAG 8 cut(s) 86, 834, 1539, 2235, 2828, 3105, 3470, 3479
GlaI GCGC 5 cut(s) 21, 2106, 2133, 2420, 3586
GsaI CCCAGC 2 cut(s) 1939, 3208
GsuI CTGGAG 2 cut(s) 782, 1103
HaeII RGCGCY 1 cut(s) 2422
HaeIII GGCC 4 cut(s) 749, 1910, 3302, 3525
HgaI GACGC 2 cut(s) 1368, 1757
HhaI GCGC 5 cut(s) 22, 2107, 2134, 2421, 3587
Hin1I GRCGYC 2 cut(s) 1360, 2343
Hin6I GCGC 5 cut(s) 20, 2105, 2132, 2419, 3585
HinP1I GCGC 5 cut(s) 20, 2105, 2132, 2419, 3585
HincII GTYRAC 3 cut(s) 535, 673, 2689
HindII GTYRAC 3 cut(s) 535, 673, 2689
HpaI GTTAAC 1 cut(s) 673
HphI GGTGA 8 cut(s) 58, 1065, 1736, 1793, 1852, 2071, 2547, 3133
Hpy99I CGWCG 2 cut(s) 419, 1520
HpyAV CCTTC 8 cut(s) 307, 342, 667, 882, 2095, 2357, 2487, 3609
HpyCH4III ACNGT 9 cut(s) 1087, 1244, 1596, 2043, 2559, 2610, 2712, 2763, 2788
HpyCH4IV ACGT 6 cut(s) 2118, 2343, 2401, 2973, 3151, 3419
HpyF10VI GCNNNNNNNGC 9 cut(s) 194, 314, 1203, 1382, 2138, 2456, 2922, 3410, 3554
HpySE526I ACGT 6 cut(s) 2118, 2343, 2401, 2973, 3151, 3419
Hsp92I GRCGYC 2 cut(s) 1360, 2343
HspAI GCGC 5 cut(s) 20, 2105, 2132, 2419, 3585
Kpn2I TCCGGA 4 cut(s) 2701, 2752, 2810, 3504
KpnI GGTACC 1 cut(s) 1595
Ksp22I TGATCA 2 cut(s) 1993, 3517
KspAI GTTAAC 1 cut(s) 673
LmnI GCTCC 9 cut(s) 109, 734, 1561, 1693, 2138, 2728, 2755, 2779, 3610
Lsp1109I GCAGC 9 cut(s) 785, 904, 1767, 1826, 2444, 2447, 2496, 3120, 3532
MaeI CTAG 8 cut(s) 86, 834, 1539, 2235, 2828, 3105, 3470, 3479
MaeII ACGT 6 cut(s) 2118, 2343, 2401, 2973, 3151, 3419
MaeIII GTNAC 6 cut(s) 46, 1127, 1501, 2114, 3147, 3566
MfeI CAATTG 8 cut(s) 150, 177, 219, 252, 285, 1251, 3084, 3355
MflI RGATCY 4 cut(s) 385, 951, 2287, 3162
MhlI GDGCHC 1 cut(s) 619
MlyI GAGTC 8 cut(s) 530, 554, 560, 572, 578, 596, 2282, 3155
MroI TCCGGA 4 cut(s) 2701, 2752, 2810, 3504
MroXI GAANNNNTTC 1 cut(s) 3195
MslI CAYNNNNRTG 3 cut(s) 55, 1464, 2847
MspR9I CCNGG 7 cut(s) 1349, 2090, 2360, 2652, 2675, 2726, 2777
MunI CAATTG 8 cut(s) 150, 177, 219, 252, 285, 1251, 3084, 3355
Mva1269I GAATGC 2 cut(s) 1582, 2935
MvaI CCWGG 5 cut(s) 1349, 2360, 2675, 2726, 2777
MvnI CGCG 3 cut(s) 22, 2107, 2134
MwoI GCNNNNNNNGC 9 cut(s) 194, 314, 1203, 1382, 2138, 2456, 2922, 3410, 3554
NciI CCSGG 2 cut(s) 2090, 2652
NcoI CCATGG 1 cut(s) 1911
NlaIV GGNNCC 9 cut(s) 75, 105, 387, 618, 1593, 2087, 2655, 2706, 3130
NmeAIII GCCGAG 2 cut(s) 2901, 3328
NmuCI GTSAC 3 cut(s) 46, 1501, 3147
NspI RCATGY 8 cut(s) 60, 83, 144, 171, 213, 279, 353, 2459
PaeI GCATGC 1 cut(s) 2459
PaeR7I CTCGAG 1 cut(s) 2999
PagI TCATGA 2 cut(s) 2817, 3457
PciI ACATGT 1 cut(s) 349
PctI GAATGC 2 cut(s) 1582, 2935
PdmI GAANNNNTTC 1 cut(s) 3195
Pfl23II CGTACG 1 cut(s) 1570
PfoI TCCNGGA 2 cut(s) 2358, 2650
Ple19I CGATCG 1 cut(s) 479
PleI GAGTC 8 cut(s) 530, 554, 560, 572, 578, 596, 2281, 3154
PmaCI CACGTG 1 cut(s) 3152
PmlI CACGTG 1 cut(s) 3152
PpsI GAGTC 8 cut(s) 530, 554, 560, 572, 578, 596, 2281, 3154
Ppu21I YACGTR 2 cut(s) 2974, 3152
PscI ACATGT 1 cut(s) 349
Psp1406I AACGTT 1 cut(s) 2401
Psp6I CCWGG 5 cut(s) 1347, 2358, 2673, 2724, 2775
PspCI CACGTG 1 cut(s) 3152
PspFI CCCAGC 2 cut(s) 1935, 3204
PspGI CCWGG 5 cut(s) 1347, 2358, 2673, 2724, 2775
PspLI CGTACG 1 cut(s) 1570
PspN4I GGNNCC 9 cut(s) 75, 105, 387, 618, 1593, 2087, 2655, 2706, 3130
PspPI GGNCC 3 cut(s) 555, 2654, 3523
PstI CTGCAG 1 cut(s) 988
PstNI CAGNNNCTG 3 cut(s) 805, 2731, 2782
PsuI RGATCY 4 cut(s) 385, 951, 2287, 3162
PvuI CGATCG 1 cut(s) 479
RseI CAYNNNNRTG 3 cut(s) 55, 1464, 2847
SalI GTCGAC 1 cut(s) 533
Sau96I GGNCC 3 cut(s) 555, 2654, 3523
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 8 cut(s) 530, 554, 560, 572, 578, 596, 2282, 3155
ScrFI CCNGG 7 cut(s) 1349, 2090, 2360, 2652, 2675, 2726, 2777
SduI GDGCHC 1 cut(s) 619
SfcI CTRYAG 3 cut(s) 459, 984, 3255
Sfr274I CTCGAG 1 cut(s) 2999
SinI GGWCC 2 cut(s) 555, 2654
SlaI CTCGAG 1 cut(s) 2999
SmiI ATTTAAAT 1 cut(s) 2392
SmiMI CAYNNNNRTG 3 cut(s) 55, 1464, 2847
SmlI CTYRAG 3 cut(s) 1904, 2999, 3296
SmoI CTYRAG 3 cut(s) 1904, 2999, 3296
SpeI ACTAGT 1 cut(s) 3478
SphI GCATGC 1 cut(s) 2459
SspI AATATT 3 cut(s) 111, 1739, 3223
SspMI CTAG 8 cut(s) 86, 834, 1539, 2235, 2828, 3105, 3470, 3479
StyD4I CCNGG 7 cut(s) 1347, 2088, 2358, 2650, 2673, 2724, 2775
StyI CCWWGG 3 cut(s) 809, 1911, 2827
SwaI ATTTAAAT 1 cut(s) 2392
TaaI ACNGT 9 cut(s) 1087, 1244, 1596, 2043, 2559, 2610, 2712, 2763, 2788
TaiI ACGT 6 cut(s) 2121, 2346, 2404, 2976, 3154, 3422
TaqI TCGA 8 cut(s) 414, 534, 1092, 1215, 1515, 2350, 3000, 3351
TatI WGTACW 3 cut(s) 499, 1082, 1687
TauI GCSGC 1 cut(s) 104
TscAI CASTG 9 cut(s) 126, 140, 387, 653, 988, 1996, 2039, 2647, 3347
TseFI GTSAC 3 cut(s) 46, 1501, 3147
TseI GCWGC 9 cut(s) 773, 917, 1755, 1814, 2432, 2435, 2509, 3108, 3545
Tsp45I GTSAC 3 cut(s) 46, 1501, 3147
TspGWI ACGGA 4 cut(s) 258, 291, 500, 1588
TspRI CASTG 9 cut(s) 126, 140, 387, 653, 988, 1996, 2039, 2647, 3347
VpaK11BI GGWCC 2 cut(s) 555, 2654
XagI CCTNNNNNAGG 1 cut(s) 3170
XbaI TCTAGA 1 cut(s) 3469
XceI RCATGY 8 cut(s) 60, 83, 144, 171, 213, 279, 353, 2459
XcmI CCANNNNNNNNNTGG 2 cut(s) 156, 700
XhoI CTCGAG 1 cut(s) 2999
XmaJI CCTAGG 1 cut(s) 2827
XmiI GTMKAC 5 cut(s) 534, 2586, 2637, 2739, 2790
XmnI GAANNNNTTC 1 cut(s) 3195
XspI CTAG 8 cut(s) 86, 834, 1539, 2235, 2828, 3105, 3470, 3479
ZraI GACGTC 1 cut(s) 2344
ZrmI AGTACT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.