Rw2G042900

voltage-gated potassium channel activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000491
Physical Location & Seq
Reverse (-)
69283745 .. 69288053
4309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G042900.1

Sequence Viewer

Length: 2517 bp
ATGGATATCGAGGAAGTCCCAGACTTCAGCGTTACAACACCGGATTACATTTCACAGGGTAGTTTAGGGCAAAATCCGATTTCTGTAACTCCAGTAACTCCAATGAGGAAAGCATGGTCAAAAGTTACTCTAATTTGGGATATATATTCAGCGGGAACAACTATAATGGTGAAGATAATATTTTCAAGAGTAATTCGCTCTATACATGAAATTCTAAGTTGGAAATGGATATTTGTGGTATCATGCATTGCAGTCTCATTGGATCCTTTGTTCCTTTACATTCCGATCATCGAGGGAAACAAGTGCCTTGGAACCGACAGAAATTTGAGGATAGTAACTCTAGTTCTCCGATCACTTATAGATATCACTTTCATAACTCACATTATTGGTCTAATTTCTGATTGGAAATACTCTGACGGCCCCCAAAGGCATGTCTCCTACTTCGACTCTCACTTTGAGCCAGCATCAGGCTTGCTTTGGAAACTAAAGTTCCTTAAACAAGCCATAGCTAAGAGGATGCCATGGTTATCTGGTACTGTCATGATCCACTTTCTTGCTATTCTTCCTATCCCACAGGTGGCAATAGTATATGTCTTTGCTCGAATTGGAAGCTCTGAATTTTTTCTCAAAACTGCGATTATCAACTTATTTCTTGTCTTCCAATATTTCCCGAGGGTTTGCCAAATCTACCTATCAAGTAAGAAACTCAACATGACCGGAACATGGGTTAAAGGTGGATTCAATTTGTTTTTGTATATCCTTGCAAGTCATGTTATTGCAGCCTACTGGTATTTTTTTGCAATTCAGAGAGAGATCTCATGTTGGCACCAGGCGTGTGAAAAAAGTGCAGGATGCCTAGCAACTTATGCTTGTGGTGGCAGTACTTCAATAAATATCACATTCCAAAATGAGCTATGCCCAATATTTTCACCAAATACTACATTATTTGATTTTGGAATATTTCTTGATATTCTTCAGTCTGGCAATGCAGGATCAGTAAATTTCTTGACAAAATTCTGTTACTCTCTATGGTGGGGACTAAAAAATCTAAGTAGTTTTGGCACAAATCTCGAAACAAGTAGCTATTGGTGGGAAAATTGTTTTGCAATTCTTATTTGTGTTGTTGGCTTGGTATTATTTTTGCACCTCGTTGGAAATGTACAGACATATATGCAGTTTCAAACTACAAAATCTGCGAGCATTGCTGATTCAAGACGGACTAGGATTAAAGCGAAGTATCGAGATATTAATAAGTGGATGTCAAAGCATGGTCTCCCTGCAGATATGAAGACAGCAATCAGGAAAGACATAAAAGAATGGAACATAGCCGGGAGATACATAGATCATGATGTGGATGTTCGATTTGTCAATAACATTTGTGGAGGAAACATCGTATTAGTCCTGTCGGAATTTTTCCGGCTAAATGCTCTAAAGAAAGTACCCATGCTTCAAAATGTGCCAGAGTTCGGGTTGATACTTATCAGCCAATATCTCTATCTAGAAACCTACGATGAGAATGCAGTTGTTGCCCGAGCAGGAAAACCGATTGATTCGATGTTCTTCATTATAGATGGCGTGATTGATTGGACTGACGAGACTAACATGGATACCGATGCTGAAACCACGGGTTCTTCTTCCTCATCTAATAGCAGCAAGCTTAAGAACGGTGATTATTTTGGACAAGAACTTTTGAGTTGGGCAGCATTTAACTATGGGAGCTCTTATTTTAGCCCTGTTCCTACTTCGACTCGAGATGCCAAATGTCGAACAAAAGTAGAAGTCCTGCAGCTGAGTAGGGGGACCTTAGTAAATACAGAAGTCTGCGATTACATGCCTCAGAGTGATTCTCTGGCGCACTTGGAGGAGTTGGCAGTTCCCCATAACCACATTGATCAGATAATAGACGAGTATCCAGTTCCTAAGGTGAAATGGAAATGTCCTTACCGTGGGTGGCTGAAAGTGAACTGTAGTGCTGTGTGGGAACGTGAATCAAGAGTGGCAGGCATGGCTTGGGTTTGCAGGAAAGATGAAGGAGCTTTCGTGGCCGCTGGAAGTCTTGGTGGGATAAGATGCATGGACAGGTTTTTCGCTGAAGCTCTCAGCATCGCTAAGGCTATCGAGTTTTGTGCCACTATTATTAGTGATGTTTCTGGCGACAACGTCGGCCGCATAATTGTGGAGTCTGATAACCAGGATCTCATGGATTGCTTGCTTCAGCTGCATGACGAGGACCTAGTTGTACCGAAAACATACTTGAACAACTCTAGCTTGGAACCGAATAACCGTATTGGCGAAAACCTTCGACCAATCCTTGGGGATATTTTCATCCGCTTGGCTCCAGGGTTTAAGGCTATTACTTACGTGCATTGTGATAGCCAATGTAATGCAGCTGCTCATTGCGTAGCATCTCATGCCATGGATGATGCTACTGATTCTTCATCTTGGTATGGTCCCGATCCGGCCCCTAGTTGGCTAATTCCTGCTCTCACGAAGGATAGCCCGCATTTGTACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
6PF2K Ank_2 Ank_4 Ank_5 F-box-like His_Phos_1 LRR_6 PAS_9 Pkinase Pkinase_Tyr RGS cNMP_binding

Protein Analysis

838

Amino Acids

94.33

Weight (kDa)

6.24

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 825
AccB7I CCANNNNNTGG 1 cut(s) 2312
AciI CCGC 5 cut(s) 152, 2046, 2167, 2329, 2501
AclWI GGATC 6 cut(s) 257, 270, 538, 1000, 2202, 2450
AcoI YGGCCR 2 cut(s) 2043, 2164
AcsI RAATTY 6 cut(s) 210, 322, 617, 1000, 1013, 1409
AcuI CTGAAG 4 cut(s) 10, 959, 2112, 2198
AfaI GTAC 6 cut(s) 535, 883, 1161, 1440, 2241, 2510
AfiI CCNNNNNNNGG 7 cut(s) 467, 577, 723, 1466, 1946, 2312, 2469
AflII CTTAAG 1 cut(s) 1658
AgsI TTSAA 7 cut(s) 186, 742, 888, 1181, 1212, 1451, 2257
AjnI CCWGG 3 cut(s) 828, 2190, 2338
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
Alw21I GWGCWC 1 cut(s) 1721
Alw26I GTCTC 4 cut(s) 259, 439, 1277, 1589
AlwI GGATC 6 cut(s) 257, 270, 538, 1000, 2202, 2450
Ama87I CYCGRG 3 cut(s) 670, 1530, 1749
AoxI GGCC 4 cut(s) 418, 2043, 2164, 2460
ApeKI GCWGC 7 cut(s) 779, 1650, 1700, 1786, 2218, 2387, 2390
ApoI RAATTY 6 cut(s) 210, 322, 617, 1000, 1013, 1409
ArsI GACNNNNNNTTYG 4 cut(s) 437, 469, 2069, 2101
AseI ATTAAT 1 cut(s) 1248
Asp700I GAANNNNTTC 2 cut(s) 621, 2298
AspLEI GCGC 1 cut(s) 1855
AspS9I GGNCC 5 cut(s) 419, 1800, 2230, 2450, 2461
AsuC2I CCSGG 1 cut(s) 1330
AsuHPI GGTGA 4 cut(s) 181, 921, 1679, 1936
AvaI CYCGRG 3 cut(s) 670, 1530, 1749
AvaII GGWCC 3 cut(s) 1800, 2230, 2450
AxyI CCTNAGG 1 cut(s) 1920
BamHI GGATCC 1 cut(s) 262
BanI GGYRCC 1 cut(s) 825
BanII GRGCYC 1 cut(s) 1721
BarI GAAGNNNNNNTAC 2 cut(s) 1431, 1463
BbsI GAAGAC 2 cut(s) 649, 1295
Bbv12I GWGCWC 1 cut(s) 1721
BbvI GCAGC 7 cut(s) 791, 1662, 1712, 1798, 2205, 2377, 2399
BccI CCATC 1 cut(s) 1565
BceAI ACGGC 1 cut(s) 433
BcgI CGANNNNNNTGC 6 cut(s) 1051, 1085, 1499, 1533, 2108, 2142
BciT130I CCWGG 3 cut(s) 830, 2192, 2340
BciVI GTATCC 2 cut(s) 1600, 1920
BclI TGATCA 1 cut(s) 1891
BcnI CCSGG 1 cut(s) 1330
BcoDI GTCTC 4 cut(s) 259, 439, 1277, 1589
BfaI CTAG 7 cut(s) 341, 857, 1221, 1499, 2234, 2265, 2466
BfmI CTRYAG 3 cut(s) 1278, 1784, 1966
BfrI CTTAAG 1 cut(s) 1658
BfuI GTATCC 2 cut(s) 1600, 1920
BglII AGATCT 1 cut(s) 813
BisI GCNGC 9 cut(s) 780, 1651, 1701, 1787, 2046, 2167, 2219, 2388, 2391
BlsI GCNGC 9 cut(s) 781, 1652, 1702, 1788, 2047, 2168, 2220, 2389, 2392
BmcAI AGTACT 1 cut(s) 883
Bme1390I CCNGG 4 cut(s) 830, 1330, 2192, 2340
Bme18I GGWCC 3 cut(s) 1800, 2230, 2450
BmeT110I CYCGRG 3 cut(s) 670, 1530, 1749
BmgT120I GGNCC 5 cut(s) 419, 1800, 2230, 2450, 2461
BmiI GGNNCC 9 cut(s) 264, 313, 421, 827, 1801, 2274, 2337, 2452, 2463
BmrFI CCNGG 4 cut(s) 830, 1330, 2192, 2340
BmsI GCATC 9 cut(s) 473, 507, 842, 1603, 1744, 2060, 2112, 2413, 2414
BpiI GAAGAC 2 cut(s) 649, 1295
BplI GAGNNNNNCTC 2 cut(s) 1831, 1863
BpmI CTGGAG 2 cut(s) 75, 2322
Bpu10I CCTNAGC 1 cut(s) 2109
BpuMI CCSGG 1 cut(s) 1330
BsaAI YACGTR 1 cut(s) 2362
BsaBI GATNNNNATC 1 cut(s) 1478
BsaI GGTCTC 1 cut(s) 1277
BsaJI CCNNGG 8 cut(s) 307, 521, 671, 1623, 1945, 2311, 2339, 2415
BsaWI WCCGGW 2 cut(s) 40, 716
BsaXI ACNNNNNCTCC 2 cut(s) 2025, 2055
Bsc4I CCNNNNNNNGG 7 cut(s) 467, 577, 723, 1466, 1946, 2312, 2469
Bse1I ACTGG 3 cut(s) 92, 791, 1913
Bse21I CCTNAGG 1 cut(s) 1920
Bse3DI GCAATG 4 cut(s) 246, 991, 1200, 2395
Bse8I GATNNNNATC 1 cut(s) 1478
BseBI CCWGG 3 cut(s) 830, 2192, 2340
BseDI CCNNGG 8 cut(s) 307, 521, 671, 1623, 1945, 2311, 2339, 2415
BseGI GGATG 6 cut(s) 522, 857, 1263, 1360, 2325, 2425
BseJI GATNNNNATC 1 cut(s) 1478
BseLI CCNNNNNNNGG 7 cut(s) 467, 577, 723, 1466, 1946, 2312, 2469
BseMI GCAATG 4 cut(s) 246, 991, 1200, 2395
BseMII CTCAG 3 cut(s) 1781, 1850, 2113
BseNI ACTGG 3 cut(s) 92, 791, 1913
BseRI GAGGAG 1 cut(s) 1877
BseX3I CGGCCG 1 cut(s) 2164
BseXI GCAGC 7 cut(s) 791, 1662, 1712, 1798, 2205, 2377, 2399
BsgI GTGCAG 1 cut(s) 867
Bsh1285I CGRYCG 1 cut(s) 2167
BshFI GGCC 4 cut(s) 420, 2045, 2166, 2462
BshNI GGYRCC 1 cut(s) 825
BsiEI CGRYCG 1 cut(s) 2167
BsiHKAI GWGCWC 1 cut(s) 1721
BsiHKCI CYCGRG 3 cut(s) 670, 1530, 1749
BsiSI CCGG 5 cut(s) 41, 717, 1329, 1417, 2459
BslFI GGGAC 4 cut(s) 2, 1050, 1813, 2436
BslI CCNNNNNNNGG 7 cut(s) 467, 577, 723, 1466, 1946, 2312, 2469
BsmAI GTCTC 4 cut(s) 259, 439, 1277, 1589
BsmFI GGGAC 4 cut(s) 2, 1050, 1813, 2436
BsmI GAATGC 1 cut(s) 1522
BsnI GGCC 4 cut(s) 420, 2045, 2166, 2462
Bso31I GGTCTC 1 cut(s) 1277
BsoBI CYCGRG 3 cut(s) 670, 1530, 1749
Bsp1286I GDGCHC 1 cut(s) 1721
Bsp1407I TGTACA 2 cut(s) 1159, 2508
Bsp19I CCATGG 2 cut(s) 521, 2415
BspACI CCGC 5 cut(s) 152, 2046, 2167, 2329, 2501
BspANI GGCC 4 cut(s) 420, 2045, 2166, 2462
BspCNI CTCAG 3 cut(s) 1782, 1849, 2112
BspHI TCATGA 2 cut(s) 540, 1345
BspLI GGNNCC 9 cut(s) 264, 313, 421, 827, 1801, 2274, 2337, 2452, 2463
BspMAI CTGCAG 2 cut(s) 1282, 1788
BspPI GGATC 6 cut(s) 257, 270, 538, 1000, 2202, 2450
BspT107I GGYRCC 1 cut(s) 825
BspTI CTTAAG 1 cut(s) 1658
BspTNI GGTCTC 1 cut(s) 1277
BsrDI GCAATG 4 cut(s) 246, 991, 1200, 2395
BsrGI TGTACA 2 cut(s) 1159, 2508
BsrI ACTGG 3 cut(s) 92, 791, 1913
BssECI CCNNGG 8 cut(s) 307, 521, 671, 1623, 1945, 2311, 2339, 2415
BssT1I CCWWGG 4 cut(s) 307, 521, 2311, 2415
Bst2UI CCWGG 3 cut(s) 830, 2192, 2340
Bst4CI ACNGT 5 cut(s) 538, 1667, 1946, 1967, 2285
BstAFI CTTAAG 1 cut(s) 1658
BstAPI GCANNNNNTGC 3 cut(s) 866, 1526, 2411
BstAUI TGTACA 2 cut(s) 1159, 2508
BstBAI YACGTR 1 cut(s) 2362
BstC8I GCNNGC 7 cut(s) 462, 473, 1198, 1655, 2002, 2210, 2501
BstDEI CTNAG 9 cut(s) 215, 510, 1049, 1790, 1804, 1836, 1920, 2099, 2109
BstDSI CCRYGG 4 cut(s) 521, 1623, 1945, 2415
BstF5I GGATG 6 cut(s) 522, 857, 1263, 1360, 2325, 2425
BstHHI GCGC 1 cut(s) 1855
BstMAI GTCTC 4 cut(s) 259, 439, 1277, 1589
BstMCI CGRYCG 1 cut(s) 2167
BstMWI GCNNNNNNNGC 7 cut(s) 866, 1202, 1526, 2006, 2042, 2218, 2411
BstNI CCWGG 3 cut(s) 830, 2192, 2340
BstNSI RCATGY 2 cut(s) 434, 1834
BstSCI CCNGG 4 cut(s) 828, 1328, 2190, 2338
BstSFI CTRYAG 3 cut(s) 1278, 1784, 1966
BstV1I GCAGC 7 cut(s) 791, 1662, 1712, 1798, 2205, 2377, 2399
BstV2I GAAGAC 2 cut(s) 649, 1295
BstX2I RGATCY 3 cut(s) 262, 813, 2194
BstYI RGATCY 3 cut(s) 262, 813, 2194
BstZI CGGCCG 1 cut(s) 2164
Bsu36I CCTNAGG 1 cut(s) 1920
BsuI GTATCC 2 cut(s) 1600, 1920
BsuRI GGCC 4 cut(s) 420, 2045, 2166, 2462
BtgI CCRYGG 4 cut(s) 521, 1623, 1945, 2415
BtgZI GCGATG 1 cut(s) 2089
BtsCI GGATG 6 cut(s) 522, 857, 1263, 1360, 2325, 2425
Cac8I GCNNGC 7 cut(s) 462, 473, 1198, 1655, 2002, 2210, 2501
CciI TCATGA 2 cut(s) 540, 1345
CfoI GCGC 1 cut(s) 1855
Cfr13I GGNCC 5 cut(s) 419, 1800, 2230, 2450, 2461
Csp6I GTAC 6 cut(s) 534, 882, 1160, 1439, 2240, 2509
CviQI GTAC 6 cut(s) 534, 882, 1160, 1439, 2240, 2509
DdeI CTNAG 9 cut(s) 215, 510, 1049, 1790, 1804, 1836, 1920, 2099, 2109
EaeI YGGCCR 2 cut(s) 2043, 2164
EagI CGGCCG 1 cut(s) 2164
Ecl136II GAGCTC 1 cut(s) 1719
EclXI CGGCCG 1 cut(s) 2164
Eco130I CCWWGG 4 cut(s) 307, 521, 2311, 2415
Eco24I GRGCYC 1 cut(s) 1721
Eco31I GGTCTC 1 cut(s) 1277
Eco32I GATATC 2 cut(s) 7, 364
Eco47I GGWCC 3 cut(s) 1800, 2230, 2450
Eco52I CGGCCG 1 cut(s) 2164
Eco53kI GAGCTC 1 cut(s) 1719
Eco57I CTGAAG 4 cut(s) 10, 959, 2112, 2198
Eco81I CCTNAGG 1 cut(s) 1920
Eco88I CYCGRG 3 cut(s) 670, 1530, 1749
EcoICRI GAGCTC 1 cut(s) 1719
EcoO109I RGGNCCY 2 cut(s) 1800, 2230
EcoRII CCWGG 3 cut(s) 828, 2190, 2338
EcoRV GATATC 2 cut(s) 7, 364
EcoT14I CCWWGG 4 cut(s) 307, 521, 2311, 2415
EcoT22I ATGCAT 2 cut(s) 248, 2075
EcoT38I GRGCYC 1 cut(s) 1721
ErhI CCWWGG 4 cut(s) 307, 521, 2311, 2415
FaqI GGGAC 4 cut(s) 2, 1050, 1813, 2436
FauI CCCGC 2 cut(s) 145, 2508
FbaI TGATCA 1 cut(s) 1891
Fnu4HI GCNGC 9 cut(s) 780, 1651, 1701, 1787, 2046, 2167, 2219, 2388, 2391
FokI GGATG 6 cut(s) 529, 864, 1270, 1367, 2312, 2432
FriOI GRGCYC 1 cut(s) 1721
Fsp4HI GCNGC 9 cut(s) 780, 1651, 1701, 1787, 2046, 2167, 2219, 2388, 2391
FspBI CTAG 7 cut(s) 341, 857, 1221, 1499, 2234, 2265, 2466
GlaI GCGC 1 cut(s) 1854
GluI GCNGC 9 cut(s) 780, 1651, 1701, 1787, 2046, 2167, 2219, 2388, 2391
GsuI CTGGAG 2 cut(s) 75, 2322
HaeIII GGCC 4 cut(s) 420, 2045, 2166, 2462
HapII CCGG 5 cut(s) 41, 717, 1329, 1417, 2459
HhaI GCGC 1 cut(s) 1855
Hin6I GCGC 1 cut(s) 1853
HinP1I GCGC 1 cut(s) 1853
HindIII AAGCTT 1 cut(s) 1655
HinfI GANTC 9 cut(s) 446, 738, 1208, 1550, 1747, 1844, 1988, 2180, 2432
HpaII CCGG 5 cut(s) 41, 717, 1329, 1417, 2459
HphI GGTGA 4 cut(s) 181, 921, 1679, 1936
Hpy166II GTNNAC 2 cut(s) 1963, 2511
Hpy8I GTNNAC 2 cut(s) 1963, 2511
Hpy99I CGWCG 1 cut(s) 2165
HpyAV CCTTC 3 cut(s) 2024, 2309, 2485
HpyCH4III ACNGT 5 cut(s) 538, 1667, 1946, 1967, 2285
HpyCH4IV ACGT 3 cut(s) 1984, 2160, 2361
HpyF10VI GCNNNNNNNGC 7 cut(s) 866, 1202, 1526, 2006, 2042, 2218, 2411
HpyF3I CTNAG 9 cut(s) 215, 510, 1049, 1790, 1804, 1836, 1920, 2099, 2109
HpySE526I ACGT 3 cut(s) 1984, 2160, 2361
HspAI GCGC 1 cut(s) 1853
Ksp22I TGATCA 1 cut(s) 1891
LmnI GCTCC 3 cut(s) 1716, 2033, 2341
Lsp1109I GCAGC 7 cut(s) 791, 1662, 1712, 1798, 2205, 2377, 2399
LweI GCATC 9 cut(s) 473, 507, 842, 1603, 1744, 2060, 2112, 2413, 2414
MaeI CTAG 7 cut(s) 341, 857, 1221, 1499, 2234, 2265, 2466
MaeII ACGT 3 cut(s) 1984, 2160, 2361
MaeIII GTNAC 6 cut(s) 31, 85, 94, 124, 334, 1019
MboII GAAGA 9 cut(s) 184, 554, 649, 965, 1300, 1552, 1623, 1626, 2427
MflI RGATCY 3 cut(s) 262, 813, 2194
MhlI GDGCHC 1 cut(s) 1721
MlyI GAGTC 3 cut(s) 440, 1741, 2189
MmeI TCCRAC 3 cut(s) 200, 1132, 1386
Mph1103I ATGCAT 2 cut(s) 248, 2075
MroXI GAANNNNTTC 2 cut(s) 621, 2298
MseI TTAA 7 cut(s) 495, 729, 1227, 1248, 1659, 1707, 2346
MslI CAYNNNNRTG 1 cut(s) 2174
MspA1I CMGCKG 5 cut(s) 152, 1789, 2048, 2218, 2390
MspCI CTTAAG 1 cut(s) 1658
MspI CCGG 5 cut(s) 41, 717, 1329, 1417, 2459
MspR9I CCNGG 4 cut(s) 830, 1330, 2192, 2340
Mva1269I GAATGC 1 cut(s) 1522
MvaI CCWGG 3 cut(s) 830, 2192, 2340
MwoI GCNNNNNNNGC 7 cut(s) 866, 1202, 1526, 2006, 2042, 2218, 2411
NciI CCSGG 1 cut(s) 1330
NcoI CCATGG 2 cut(s) 521, 2415
NlaIV GGNNCC 9 cut(s) 264, 313, 421, 827, 1801, 2274, 2337, 2452, 2463
NsiI ATGCAT 2 cut(s) 248, 2075
NspI RCATGY 2 cut(s) 434, 1834
PaeR7I CTCGAG 1 cut(s) 1749
PagI TCATGA 2 cut(s) 540, 1345
PctI GAATGC 1 cut(s) 1522
PdmI GAANNNNTTC 2 cut(s) 621, 2298
PfeI GAWTC 6 cut(s) 738, 1208, 1550, 1844, 1988, 2432
PflFI GACNNNGTC 1 cut(s) 2159
PflMI CCANNNNNTGG 1 cut(s) 2312
PkrI GCNGC 9 cut(s) 781, 1652, 1702, 1788, 2047, 2168, 2220, 2389, 2392
PleI GAGTC 3 cut(s) 440, 1741, 2188
PpsI GAGTC 3 cut(s) 440, 1741, 2188
Ppu21I YACGTR 1 cut(s) 2362
PpuMI RGGWCCY 2 cut(s) 1800, 2230
PshBI ATTAAT 1 cut(s) 1248
Psp124BI GAGCTC 1 cut(s) 1721
Psp5II RGGWCCY 2 cut(s) 1800, 2230
Psp6I CCWGG 3 cut(s) 828, 2190, 2338
PspGI CCWGG 3 cut(s) 828, 2190, 2338
PspN4I GGNNCC 9 cut(s) 264, 313, 421, 827, 1801, 2274, 2337, 2452, 2463
PspPI GGNCC 5 cut(s) 419, 1800, 2230, 2450, 2461
PspPPI RGGWCCY 2 cut(s) 1800, 2230
PsrI GAACNNNNNNTAC 2 cut(s) 327, 359
PstI CTGCAG 2 cut(s) 1282, 1788
PsuI RGATCY 3 cut(s) 262, 813, 2194
PsyI GACNNNGTC 1 cut(s) 2159
PvuII CAGCTG 3 cut(s) 1789, 2218, 2390
RsaI GTAC 6 cut(s) 535, 883, 1161, 1440, 2241, 2510
RsaNI GTAC 6 cut(s) 534, 882, 1160, 1439, 2240, 2509
RseI CAYNNNNRTG 1 cut(s) 2174
SacI GAGCTC 1 cut(s) 1721
SaqAI TTAA 7 cut(s) 495, 729, 1227, 1248, 1659, 1707, 2346
SatI GCNGC 9 cut(s) 780, 1651, 1701, 1787, 2046, 2167, 2219, 2388, 2391
Sau96I GGNCC 5 cut(s) 419, 1800, 2230, 2450, 2461
ScaI AGTACT 1 cut(s) 883
SchI GAGTC 3 cut(s) 440, 1741, 2189
ScrFI CCNGG 4 cut(s) 830, 1330, 2192, 2340
SduI GDGCHC 1 cut(s) 1721
SfaNI GCATC 9 cut(s) 473, 507, 842, 1603, 1744, 2060, 2112, 2413, 2414
SfcI CTRYAG 3 cut(s) 1278, 1784, 1966
Sfr274I CTCGAG 1 cut(s) 1749
SinI GGWCC 3 cut(s) 1800, 2230, 2450
SlaI CTCGAG 1 cut(s) 1749
SmiMI CAYNNNNRTG 1 cut(s) 2174
SmlI CTYRAG 2 cut(s) 1658, 1749
SmoI CTYRAG 2 cut(s) 1658, 1749
SsiI CCGC 5 cut(s) 152, 2046, 2167, 2329, 2501
SspI AATATT 4 cut(s) 180, 665, 924, 960
SspMI CTAG 7 cut(s) 341, 857, 1221, 1499, 2234, 2265, 2466
SstI GAGCTC 1 cut(s) 1721
StyD4I CCNGG 4 cut(s) 828, 1328, 2190, 2338
StyI CCWWGG 4 cut(s) 307, 521, 2311, 2415
TaaI ACNGT 5 cut(s) 538, 1667, 1946, 1967, 2285
TaiI ACGT 3 cut(s) 1987, 2163, 2364
TatI WGTACW 3 cut(s) 881, 1159, 2508
TauI GCSGC 2 cut(s) 2048, 2169
TfiI GAWTC 6 cut(s) 738, 1208, 1550, 1844, 1988, 2432
Tru1I TTAA 7 cut(s) 495, 729, 1227, 1248, 1659, 1707, 2346
Tru9I TTAA 7 cut(s) 495, 729, 1227, 1248, 1659, 1707, 2346
TseI GCWGC 7 cut(s) 779, 1650, 1700, 1786, 2218, 2387, 2390
TspDTI ATGAA 7 cut(s) 222, 361, 1301, 1552, 2043, 2314, 2427
TspGWI ACGGA 1 cut(s) 1231
Tth111I GACNNNGTC 1 cut(s) 2159
Van91I CCANNNNNTGG 1 cut(s) 2312
Vha464I CTTAAG 1 cut(s) 1658
VpaK11BI GGWCC 3 cut(s) 1800, 2230, 2450
VspI ATTAAT 1 cut(s) 1248
XapI RAATTY 6 cut(s) 210, 322, 617, 1000, 1013, 1409
XbaI TCTAGA 1 cut(s) 1498
XceI RCATGY 2 cut(s) 434, 1834
XhoI CTCGAG 1 cut(s) 1749
XmnI GAANNNNTTC 2 cut(s) 621, 2298
XspI CTAG 7 cut(s) 341, 857, 1221, 1499, 2234, 2265, 2466
ZrmI AGTACT 1 cut(s) 883
Zsp2I ATGCAT 2 cut(s) 248, 2075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.