RLG00000032478

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000491
Physical Location & Seq
Forward (+)
15704642 .. 15713503
8862 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032478

Sequence Viewer

Length: 2922 bp
ATGGCTGGATCGATTTCATATCCGGGTTGCGAAGTTGATGATGACCGATATACACATATATGGCTCGAAGCGTTGAAAACCCCGGCGGTGGCACTCGTGGAAATTCCGAATCTGGCGAGGGTAAAGGTCCCTCAGTCACTGAACTCTCTTGCACGTGAACTATACGAAGTACTGCTCTCATTGTTGGGGCTAAACCTTTCCGCATCCAAGTTCAGAACATTGCCGCTGGTACATACTAATCTTGTCCATCATATATTCATATGTATGGATCAGCCAGAGGTCCTAGACGTAAGCGTTACACCACCAGCACGTTACAACTTCGACGATGTCGGGGGACCCAGTCCCAAGACTGCAACTCCAATTAGGAAAGCATGGTGGTCGGCAGTTATTAGTCGAATTTGGGAAATATTGTCAGAAGCAACCATTCTAATATGGCAAATACTGCTATATGTTACAACTCTGATTTTGAGAAGGACATGGTCAACAGTGGCAGATGGAGTCGTGATATTATACAGAGTCGCATATTTAATCTGTGAACATATAATTCGCCTCATCTTACTGCCTTCAGTGAAGTGGGAAGGGGCATTCGTAATATCATGTATTGCTGTCTCATTAGATCCTTTATTCTTATACATTCCGATAATCGACGAGACGAACAAGTGCCTTGCAGTGGACAAAATATTGAGAACTACAGCTGTTGTCCTGCGATTACTTACAGATATCCCTTTAACACTCCATTTTATAGCTCAACTTTGTGATAGGGTCGATACCGACCATCCCAACTTGTCCCACTCCGATTCTGATCACTCAGACTCAGACTCCAGCTCAGACTCTGACTTCGAGACAACATCATGTTCACTAAAATCTGTTAAAAAGAGGACAGCTAGGAGGATGCCGTGGTTATCTGTTTCTATTGTAATTAACTTTCTGGCTGTACTTCCAATCCCACAGGTGGTAATAATACTTGGCTTTTTTAGAATGAAAGGCTCATCAAGCACTGAAGTCTTTTTCCAAAGAACAATTCTCAACATTTCTCTTGTCCTCCAATATCTGCCGAGGGTTTGTCAAATTTACCTATCCAGTCGACATTTTAGAATGGGAGGAATATGGATCAAACCTACACTGAATTTAATTCTTTACATCGTCGCTGGTCACGTATTTGCTGCATTTTGGTACTTTTTTGCTATTGAACGAGTGACAGCATGTTGGCATCATGTTTGTAGAAGTACTCCAGGATGTGTAGCTACTTTTGATTGCGGTGACAATGGAAATACTTCAATAAATATAACCGATCAATTATGCCCTATAAACTCACCGAATACAACAGTATTTGATTTTGGAATTTACCTTCCAATCCTCCAATCTGGTAATATGGGATCCGTAAATTTTCTAAGAAAGCTCTTCTACTCTTTATGGTGGGGCTTAAGAAATCTAAGCTCATATGGCATGACATCATTTACTGAAACGTATTCTATACCACATAAGGGAAGACAATGTTGCAAATATATGGAGTTGGCGACTACAAAATCATTGAAGTCTGAGCAATCAAGGCGCAAGAAGATTGCAGCAAAGAAAAAAGAAATAAAATTGTGGATGTCAACTAACAATGTTTCTGTAATGTCAAGTAATGGGGTCTCTCTGAATATGAGAAAAAGAATCAAGAAATACATCAAACAACACCACATTGTGGAGAAGGACATAAAGGCCGATGTAGATCTGAAATATTTTCTAGATGATAATCGTTTTGCCACTGATCGTATCAGAGAGCCTCTGTGGGAGTATCTGGTCAAGAATGCAGCGGTAAAAAGTGTACCCATGCTTAAAAGGATGGATCAAAGCGGATGGAAAAGATTGTGCAGATATATGAAGCCGGTAATCTACAAAGAGAAAGAGGACATTGTAGAAAAGGCAGGAGAAAAGCTTGTTCAGATGATCTTCATAATACAAGGTGAATTTGAGTGCGCCAACACAATGCTCACCCACCCTAATCATCTAAAGAAAGGTGATTGTTATGGATCAATACAAATTCAAAACTGGGCAGCAAGGAACTACATGAGGTCTTATTATGACCCTCTTCCTACTTCCGCCGGACGGGTCAGATGTCAAACAAAAGTTGAAGCCTTTGCACTCCGGGCCTTGGACTTCTTACGTTTTGTCCAGCAGAATTTGAACCACTTAAAAATTAAGTCTGTGCAGTTGAAAAAGTTGGCAGCGGCCGACGACATCAGTACTCCTCATCAGACACACAACCAGGATGATCAACATTCTGTGGAGTCATGGGAATGTCCTAAGAGTGGCCTGGTGAAACTCAACTATAGTGTTGTCTGGGATTCTGTTTCTAAGAAGGCCAAAATGGGCTGCGTTTGCAGGGATAACCGAGGAGCGTTCATGCTTGCTGGAAACAGCTGTACTTCACCATATAACGACGCCTTTCTTGCTGAAGCTTCCGGCATGGCCAAGGCTATCGAGTTCTGTCACCAGAATCGCCAGAATGTTATACTCCAAGCCGGCATTGGGATTGTTGTGGAGTCTGATAACCATGATCTCATTGATTGCCTCAATCGGCCTTTGGCTTCAAATGAAAGAATTAGTGAAACCCTTCGCCCCATTCTTGAAAGAATTCTAAATTTGGCTTCGAACTTTAAACCTAGATCTTTTATGTATTGTAACCGCCAAAGTAATACAGTAGCGAACCGCCTTGCAAACCATGCGGTGGATGCTGAGGATCCTAATGTTTCAGCTTTCCGTGAAGCTCCCCCTGATTGGCTAATTCCTGCTCTCAAGGAGGATAGCCCGGATCTTTACACAGAACACGACGAGGAAATGGGTCGAAACAATGACTTCGGAGCAACTTCTCCAGATGCTGCTGCTAATGACGATGAGGACAGATATGCAATAATGGCAGAGGAGTCTCAGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

974

Amino Acids

110.37

Weight (kDa)

8.45

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 771 - 904 3.4e-13 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000491)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12100 FvH4_3g12110 FvH4_3g12111 FvH4_3g12120 FvH4_3g12120 FvH4_3g28952 FvH4_6g40660 FvH4_6g40661 FvH4_6g40960
rosa_chinensis RchiOBHm_Chr1g0340941 RchiOBHm_Chr2g0156041 RchiOBHm_Chr2g0156591 RchiOBHm_Chr5g0019791 RchiOBHm_Chr5g0019821
rosa_laevigata RLG00000007998 RLG00000020883 RLG00000020887 RLG00000020922 RLG00000029052 RLG00000032473 RLG00000032474 RLG00000032477 RLG00000032478
rosa_multiflora Rmu_co8231441.1_g000001 Rmu_co8352639.1_g000001 Rmu_co8510723.1_g000001 Rmu_sc0000344.1_g000022 Rmu_sc0000344.1_g000023 Rmu_sc0001145.1_g000015 Rmu_sc0001145.1_g000016 Rmu_sc0001211.1_g000087 Rmu_sc0001319.1_g000003 Rmu_sc0008321.1_g000001 Rmu_sc0011512.1_g000007 Rmu_sc0011922.1_g000003 Rmu_sc0011922.1_g000007 Rmu_ssc0000155.1_g000002 Rmu_ssc0000155.1_g000004
rosa_roxburghii Rroxscaffold_1G00058320 Rroxscaffold_1G00058340 Rroxscaffold_2G00092930 Rroxscaffold_2G00093240 Rroxscaffold_2G00093270 Rroxscaffold_4G00311560 Rroxscaffold_4G00311590 Rroxscaffold_5G00360130
rosa_rugosa Rorug01G0160700.1 Rorug02G0451400 Rorug02G0454400 Rorug02G0454400 Rorug02G0454500 Rorug02G0454600 Rorug05G0054100 Rorug05G0054200 Rorug05G0054300
rosa_samantha Rh1AG175400 Rh1BG143700 Rh1BG143800 Rh1CG163400 Rh1DG175300 Rh1DG175400 Rh1DG175500 Rh2AG518200 Rh2AG518300 Rh2BG529400 Rh2BG532700 Rh2BG533200 Rh2CG502900 Rh2CG503000 Rh2DG538500 Rh2DG538600 Rh2DG541600 Rh2DG542000 Rh4BG200000 Rh4BG200100 Rh5AG144500 Rh5AG144600 Rh5AG366400 Rh5BG143500 Rh5BG143700 Rh5CG155000 Rh5CG155300 Rh5DG143500 Rh7BG257300 Rh7BG257400 Rh7BG257500
rosa_wichuraiana Rw1G014680 Rw2G042670 Rw2G042900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1687, 2713
AccI GTMKAC 1 cut(s) 1084
AcoI YGGCCR 2 cut(s) 2214, 2454
AcuI CTGAAG 3 cut(s) 549, 1020, 2460
AcvI CACGTG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 2427
AdeI CACNNNGTG 1 cut(s) 1687
AfaI GTAC 8 cut(s) 171, 231, 936, 1175, 1228, 1812, 2230, 2410
AflII CTTAAG 1 cut(s) 1423
AjnI CCWGG 3 cut(s) 1231, 2250, 2298
AjuI GAANNNNNNNTTGG 2 cut(s) 1005, 1037
Alw26I GTCTC 5 cut(s) 613, 644, 836, 1639, 2916
AlwNI CAGNNNCTG 3 cut(s) 139, 833, 2864
AoxI GGCC 7 cut(s) 1704, 2133, 2214, 2296, 2346, 2454, 2564
ApeKI GCWGC 8 cut(s) 1163, 1565, 1796, 2039, 2210, 2358, 2864, 2867
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
Asp700I GAANNNNTTC 3 cut(s) 1273, 2598, 2619
AspLEI GCGC 2 cut(s) 1554, 1964
AspS9I GGNCC 4 cut(s) 127, 280, 335, 2133
AsuC2I CCSGG 4 cut(s) 24, 83, 2132, 2795
AsuHPI GGTGA 8 cut(s) 1271, 1305, 1961, 1969, 2015, 2314, 2406, 2468
AsuII TTCGAA 1 cut(s) 2636
AvaII GGWCC 3 cut(s) 127, 280, 335
BalI TGGCCA 1 cut(s) 2456
BamHI GGATCC 2 cut(s) 1376, 2725
BauI CACGAG 1 cut(s) 95
BbrPI CACGTG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 1495
BbvCI CCTCAGC 1 cut(s) 2721
BbvI GCAGC 8 cut(s) 1150, 1577, 1808, 2051, 2222, 2345, 2851, 2854
BccI CCATC 5 cut(s) 255, 488, 783, 1822, 1836
BceAI ACGGC 1 cut(s) 880
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 3 cut(s) 1233, 2252, 2300
BclI TGATCA 2 cut(s) 802, 2257
BcnI CCSGG 4 cut(s) 24, 83, 2132, 2795
BcoDI GTCTC 5 cut(s) 613, 644, 836, 1639, 2916
BfaI CTAG 4 cut(s) 284, 885, 1730, 2649
BfmI CTRYAG 2 cut(s) 690, 2314
BfrI CTTAAG 1 cut(s) 1423
BglII AGATCT 2 cut(s) 1714, 2651
BmcAI AGTACT 3 cut(s) 171, 1228, 2230
Bme1390I CCNGG 7 cut(s) 24, 83, 1233, 2132, 2252, 2300, 2795
Bme18I GGWCC 3 cut(s) 127, 280, 335
BmgT120I GGNCC 4 cut(s) 127, 280, 335, 2133
BmiI GGNNCC 5 cut(s) 129, 336, 337, 1378, 2727
BmrFI CCNGG 7 cut(s) 24, 83, 1233, 2132, 2252, 2300, 2795
BmrI ACTGGG 2 cut(s) 333, 2044
BmsI GCATC 5 cut(s) 212, 882, 1219, 2707, 2851
BmuI ACTGGG 2 cut(s) 333, 2044
BpiI GAAGAC 1 cut(s) 1495
BpmI CTGGAG 3 cut(s) 805, 1215, 2841
Bpu10I CCTNAGC 1 cut(s) 2721
Bpu14I TTCGAA 1 cut(s) 2636
BpuEI CTTGAG 1 cut(s) 2765
BpuMI CCSGG 4 cut(s) 24, 83, 2132, 2795
Bsa29I ATCGAT 1 cut(s) 11
BsaAI YACGTR 2 cut(s) 155, 1156
BsaBI GATNNNNATC 3 cut(s) 801, 1713, 1737
BsaHI GRCGYC 1 cut(s) 2427
BsaI GGTCTC 1 cut(s) 1639
BsaJI CCNNGG 6 cut(s) 81, 896, 1055, 2136, 2377, 2457
BsaXI ACNNNNNCTCC 4 cut(s) 340, 370, 803, 833
Bse118I RCCGGY 2 cut(s) 1870, 2507
Bse1I ACTGG 3 cut(s) 339, 1080, 2039
Bse3DI GCAATG 1 cut(s) 218
Bse8I GATNNNNATC 3 cut(s) 801, 1713, 1737
BseBI CCWGG 3 cut(s) 1233, 2252, 2300
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 6 cut(s) 81, 896, 1055, 2136, 2377, 2457
BseGI GGATG 9 cut(s) 203, 775, 897, 1241, 1599, 1833, 1847, 2260, 2722
BseJI GATNNNNATC 3 cut(s) 801, 1713, 1737
BseMI GCAATG 1 cut(s) 218
BseMII CTCAG 6 cut(s) 146, 822, 828, 840, 1530, 2712
BseNI ACTGG 3 cut(s) 339, 1080, 2039
BseRI GAGGAG 3 cut(s) 2223, 2394, 2921
BseX3I CGGCCG 1 cut(s) 2214
BseXI GCAGC 8 cut(s) 1150, 1577, 1808, 2051, 2222, 2345, 2851, 2854
BsgI GTGCAG 2 cut(s) 1876, 2213
Bsh1285I CGRYCG 1 cut(s) 2217
BshFI GGCC 7 cut(s) 1706, 2135, 2216, 2298, 2348, 2456, 2566
BshVI ATCGAT 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 2217
BsiSI CCGG 8 cut(s) 23, 83, 1871, 2088, 2131, 2448, 2508, 2795
BslFI GGGAC 4 cut(s) 113, 327, 348, 772
BsmAI GTCTC 5 cut(s) 613, 644, 836, 1639, 2916
BsmBI CGTCTC 1 cut(s) 644
BsmFI GGGAC 4 cut(s) 113, 327, 348, 772
BsmI GAATGC 2 cut(s) 584, 1798
BsnI GGCC 7 cut(s) 1706, 2135, 2216, 2298, 2348, 2456, 2566
Bso31I GGTCTC 1 cut(s) 1639
Bsp119I TTCGAA 1 cut(s) 2636
BspANI GGCC 7 cut(s) 1706, 2135, 2216, 2298, 2348, 2456, 2566
BspCNI CTCAG 6 cut(s) 145, 821, 827, 839, 1531, 2713
BspDI ATCGAT 1 cut(s) 11
BspLI GGNNCC 5 cut(s) 129, 336, 337, 1378, 2727
BspQI GCTCTTC 1 cut(s) 1406
BspT104I TTCGAA 1 cut(s) 2636
BspTI CTTAAG 1 cut(s) 1423
BspTNI GGTCTC 1 cut(s) 1639
BsrDI GCAATG 1 cut(s) 218
BsrFI RCCGGY 2 cut(s) 1870, 2507
BsrI ACTGG 3 cut(s) 339, 1080, 2039
BssAI RCCGGY 2 cut(s) 1870, 2507
BssECI CCNNGG 6 cut(s) 81, 896, 1055, 2136, 2377, 2457
BssNI GRCGYC 1 cut(s) 2427
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 2 cut(s) 2136, 2457
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 3 cut(s) 1233, 2252, 2300
Bst4CI ACNGT 3 cut(s) 487, 1327, 2686
Bst6I CTCTTC 2 cut(s) 1406, 2079
BstACI GRCGYC 1 cut(s) 2427
BstAFI CTTAAG 1 cut(s) 1423
BstAPI GCANNNNNTGC 2 cut(s) 442, 2708
BstBAI YACGTR 2 cut(s) 155, 1156
BstBI TTCGAA 1 cut(s) 2636
BstC8I GCNNGC 2 cut(s) 2394, 2509
BstDSI CCRYGG 1 cut(s) 896
BstF5I GGATG 9 cut(s) 203, 775, 897, 1241, 1599, 1833, 1847, 2260, 2722
BstHHI GCGC 2 cut(s) 1554, 1964
BstMAI GTCTC 5 cut(s) 613, 644, 836, 1639, 2916
BstMCI CGRYCG 1 cut(s) 2217
BstNI CCWGG 3 cut(s) 1233, 2252, 2300
BstNSI RCATGY 1 cut(s) 1206
BstSCI CCNGG 7 cut(s) 22, 81, 1231, 2130, 2250, 2298, 2793
BstSFI CTRYAG 2 cut(s) 690, 2314
BstV1I GCAGC 8 cut(s) 1150, 1577, 1808, 2051, 2222, 2345, 2851, 2854
BstV2I GAAGAC 1 cut(s) 1495
BstX2I RGATCY 6 cut(s) 616, 1376, 1714, 2651, 2725, 2797
BstYI RGATCY 6 cut(s) 616, 1376, 1714, 2651, 2725, 2797
BstZI CGGCCG 1 cut(s) 2214
Bsu15I ATCGAT 1 cut(s) 11
BsuRI GGCC 7 cut(s) 1706, 2135, 2216, 2298, 2348, 2456, 2566
BsuTUI ATCGAT 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 896
BtsCI GGATG 9 cut(s) 203, 775, 897, 1241, 1599, 1833, 1847, 2260, 2722
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 7 cut(s) 137, 492, 573, 675, 996, 1121, 1749
Cac8I GCNNGC 2 cut(s) 2394, 2509
CaiI CAGNNNCTG 3 cut(s) 139, 833, 2864
CfoI GCGC 2 cut(s) 1554, 1964
Cfr10I RCCGGY 2 cut(s) 1870, 2507
Cfr13I GGNCC 4 cut(s) 127, 280, 335, 2133
ClaI ATCGAT 1 cut(s) 11
CseI GACGC 1 cut(s) 2435
Csp6I GTAC 8 cut(s) 170, 230, 935, 1174, 1227, 1811, 2229, 2409
CviQI GTAC 8 cut(s) 170, 230, 935, 1174, 1227, 1811, 2229, 2409
DraI TTTAAA 1 cut(s) 2644
DraIII CACNNNGTG 1 cut(s) 1687
EaeI YGGCCR 2 cut(s) 2214, 2454
EagI CGGCCG 1 cut(s) 2214
Eam1104I CTCTTC 2 cut(s) 1406, 2079
EarI CTCTTC 2 cut(s) 1406, 2079
EciI GGCGGA 1 cut(s) 2074
EclXI CGGCCG 1 cut(s) 2214
Eco130I CCWWGG 2 cut(s) 2136, 2457
Eco31I GGTCTC 1 cut(s) 1639
Eco32I GATATC 1 cut(s) 721
Eco47I GGWCC 3 cut(s) 127, 280, 335
Eco52I CGGCCG 1 cut(s) 2214
Eco57I CTGAAG 3 cut(s) 549, 1020, 2460
Eco72I CACGTG 1 cut(s) 155
EcoO109I RGGNCCY 3 cut(s) 127, 280, 335
EcoRI GAATTC 1 cut(s) 2619
EcoRII CCWGG 3 cut(s) 1231, 2250, 2298
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 2 cut(s) 2136, 2457
ErhI CCWWGG 2 cut(s) 2136, 2457
Esp3I CGTCTC 1 cut(s) 644
FaqI GGGAC 4 cut(s) 113, 327, 348, 772
FauNDI CATATG 2 cut(s) 260, 1441
FbaI TGATCA 2 cut(s) 802, 2257
FblI GTMKAC 1 cut(s) 1084
FokI GGATG 9 cut(s) 190, 762, 904, 1248, 1606, 1840, 1854, 2267, 2729
FspBI CTAG 4 cut(s) 284, 885, 1730, 2649
GlaI GCGC 2 cut(s) 1553, 1963
GsuI CTGGAG 3 cut(s) 805, 1215, 2841
HaeIII GGCC 7 cut(s) 1706, 2135, 2216, 2298, 2348, 2456, 2566
HapII CCGG 8 cut(s) 23, 83, 1871, 2088, 2131, 2448, 2508, 2795
HgaI GACGC 1 cut(s) 2435
HhaI GCGC 2 cut(s) 1554, 1964
Hin1I GRCGYC 1 cut(s) 2427
Hin6I GCGC 2 cut(s) 1552, 1962
HinP1I GCGC 2 cut(s) 1552, 1962
HincII GTYRAC 3 cut(s) 483, 1085, 1599
HindII GTYRAC 3 cut(s) 483, 1085, 1599
HindIII AAGCTT 2 cut(s) 1919, 2442
HpaII CCGG 8 cut(s) 23, 83, 1871, 2088, 2131, 2448, 2508, 2795
HphI GGTGA 8 cut(s) 1271, 1305, 1961, 1969, 2015, 2314, 2406, 2468
Hpy166II GTNNAC 8 cut(s) 158, 483, 536, 673, 857, 1085, 1599, 1811
Hpy188III TCNNGA 7 cut(s) 502, 841, 1660, 1730, 1789, 2612, 2858
Hpy8I GTNNAC 8 cut(s) 158, 483, 536, 673, 857, 1085, 1599, 1811
Hpy99I CGWCG 6 cut(s) 326, 650, 1148, 2222, 2429, 2819
HpyAV CCTTC 7 cut(s) 465, 572, 573, 1358, 1687, 2338, 2609
HpyCH4III ACNGT 3 cut(s) 487, 1327, 2686
HpyCH4IV ACGT 6 cut(s) 154, 288, 310, 1155, 1466, 2149
HpySE526I ACGT 6 cut(s) 154, 288, 310, 1155, 1466, 2149
Hsp92I GRCGYC 1 cut(s) 2427
HspAI GCGC 2 cut(s) 1552, 1962
KflI GGGWCCC 1 cut(s) 335
KroI GCCGGC 1 cut(s) 2507
KroNI GCCGGC 1 cut(s) 2509
Ksp22I TGATCA 2 cut(s) 802, 2257
LguI GCTCTTC 1 cut(s) 1406
LmnI GCTCC 3 cut(s) 2381, 2758, 2846
Lsp1109I GCAGC 8 cut(s) 1150, 1577, 1808, 2051, 2222, 2345, 2851, 2854
LweI GCATC 5 cut(s) 212, 882, 1219, 2707, 2851
MaeI CTAG 4 cut(s) 284, 885, 1730, 2649
MaeII ACGT 6 cut(s) 154, 288, 310, 1155, 1466, 2149
MaeIII GTNAC 9 cut(s) 135, 295, 311, 451, 1151, 1195, 1259, 2474, 2666
MboII GAAGA 5 cut(s) 1393, 1500, 1570, 1927, 2066
MflI RGATCY 6 cut(s) 616, 1376, 1714, 2651, 2725, 2797
MlsI TGGCCA 1 cut(s) 2456
MluNI TGGCCA 1 cut(s) 2456
MlyI GAGTC 8 cut(s) 507, 525, 806, 812, 824, 2282, 2537, 2918
Mox20I TGGCCA 1 cut(s) 2456
MroNI GCCGGC 1 cut(s) 2507
MroXI GAANNNNTTC 3 cut(s) 1273, 2598, 2619
MscI TGGCCA 1 cut(s) 2456
MslI CAYNNNNRTG 3 cut(s) 58, 263, 2281
Msp20I TGGCCA 1 cut(s) 2456
MspA1I CMGCKG 5 cut(s) 226, 695, 1799, 2213, 2406
MspCI CTTAAG 1 cut(s) 1423
MspI CCGG 8 cut(s) 23, 83, 1871, 2088, 2131, 2448, 2508, 2795
MspR9I CCNGG 7 cut(s) 24, 83, 1233, 2132, 2252, 2300, 2795
Mva1269I GAATGC 2 cut(s) 584, 1798
MvaI CCWGG 3 cut(s) 1233, 2252, 2300
NaeI GCCGGC 1 cut(s) 2509
NciI CCSGG 4 cut(s) 24, 83, 2132, 2795
NdeI CATATG 2 cut(s) 260, 1441
NgoMIV GCCGGC 1 cut(s) 2507
NlaIV GGNNCC 5 cut(s) 129, 336, 337, 1378, 2727
NmeAIII GCCGAG 1 cut(s) 1080
NmuCI GTSAC 5 cut(s) 135, 1151, 1195, 1259, 2474
NspI RCATGY 1 cut(s) 1206
NspV TTCGAA 1 cut(s) 2636
PciSI GCTCTTC 1 cut(s) 1406
PcsI WCGNNNNNNNCGW 1 cut(s) 1152
PctI GAATGC 2 cut(s) 584, 1798
PdiI GCCGGC 1 cut(s) 2509
PdmI GAANNNNTTC 3 cut(s) 1273, 2598, 2619
PfeI GAWTC 5 cut(s) 109, 797, 1656, 2330, 2482
PflFI GACNNNGTC 3 cut(s) 326, 339, 478
PflMI CCANNNNNTGG 2 cut(s) 1687, 2713
PfoI TCCNGGA 1 cut(s) 1231
PleI GAGTC 8 cut(s) 506, 524, 806, 812, 824, 2281, 2536, 2917
PmaCI CACGTG 1 cut(s) 155
PmlI CACGTG 1 cut(s) 155
PpsI GAGTC 8 cut(s) 506, 524, 806, 812, 824, 2281, 2536, 2917
Ppu21I YACGTR 2 cut(s) 155, 1156
PpuMI RGGWCCY 3 cut(s) 127, 280, 335
Psp5II RGGWCCY 3 cut(s) 127, 280, 335
Psp6I CCWGG 3 cut(s) 1231, 2250, 2298
PspCI CACGTG 1 cut(s) 155
PspGI CCWGG 3 cut(s) 1231, 2250, 2298
PspN4I GGNNCC 5 cut(s) 129, 336, 337, 1378, 2727
PspPI GGNCC 4 cut(s) 127, 280, 335, 2133
PspPPI RGGWCCY 3 cut(s) 127, 280, 335
PstNI CAGNNNCTG 3 cut(s) 139, 833, 2864
PsuI RGATCY 6 cut(s) 616, 1376, 1714, 2651, 2725, 2797
PsyI GACNNNGTC 3 cut(s) 326, 339, 478
PvuII CAGCTG 2 cut(s) 695, 2406
RsaI GTAC 8 cut(s) 171, 231, 936, 1175, 1228, 1812, 2230, 2410
RsaNI GTAC 8 cut(s) 170, 230, 935, 1174, 1227, 1811, 2229, 2409
RseI CAYNNNNRTG 3 cut(s) 58, 263, 2281
SalI GTCGAC 1 cut(s) 1083
SapI GCTCTTC 1 cut(s) 1406
Sau96I GGNCC 4 cut(s) 127, 280, 335, 2133
ScaI AGTACT 3 cut(s) 171, 1228, 2230
SchI GAGTC 8 cut(s) 507, 525, 806, 812, 824, 2282, 2537, 2918
ScrFI CCNGG 7 cut(s) 24, 83, 1233, 2132, 2252, 2300, 2795
SfaNI GCATC 5 cut(s) 212, 882, 1219, 2707, 2851
SfcI CTRYAG 2 cut(s) 690, 2314
SfuI TTCGAA 1 cut(s) 2636
SinI GGWCC 3 cut(s) 127, 280, 335
SmiMI CAYNNNNRTG 3 cut(s) 58, 263, 2281
SmlI CTYRAG 2 cut(s) 1423, 2780
SmoI CTYRAG 2 cut(s) 1423, 2780
SspI AATATT 3 cut(s) 408, 681, 1724
SspMI CTAG 4 cut(s) 284, 885, 1730, 2649
StyD4I CCNGG 7 cut(s) 22, 81, 1231, 2130, 2250, 2298, 2793
StyI CCWWGG 2 cut(s) 2136, 2457
TaaI ACNGT 3 cut(s) 487, 1327, 2686
TaiI ACGT 6 cut(s) 157, 291, 313, 1158, 1469, 2152
TaqII GACCGA 1 cut(s) 60
TatI WGTACW 5 cut(s) 169, 934, 1226, 2228, 2408
TauI GCSGC 2 cut(s) 226, 2216
TfiI GAWTC 5 cut(s) 109, 797, 1656, 2330, 2482
TscAI CASTG 7 cut(s) 144, 492, 573, 675, 1003, 1128, 1756
TseFI GTSAC 5 cut(s) 135, 1151, 1195, 1259, 2474
TseI GCWGC 8 cut(s) 1163, 1565, 1796, 2039, 2210, 2358, 2864, 2867
Tsp45I GTSAC 5 cut(s) 135, 1151, 1195, 1259, 2474
TspDTI ATGAA 7 cut(s) 6, 247, 995, 1880, 1927, 2377, 2595
TspGWI ACGGA 2 cut(s) 1369, 2735
TspRI CASTG 7 cut(s) 144, 492, 573, 675, 1003, 1128, 1756
Tth111I GACNNNGTC 3 cut(s) 326, 339, 478
Van91I CCANNNNNTGG 2 cut(s) 1687, 2713
Vha464I CTTAAG 1 cut(s) 1423
VpaK11BI GGWCC 3 cut(s) 127, 280, 335
XbaI TCTAGA 1 cut(s) 1729
XceI RCATGY 1 cut(s) 1206
XcmI CCANNNNNNNNNTGG 1 cut(s) 2510
XmiI GTMKAC 1 cut(s) 1084
XmnI GAANNNNTTC 3 cut(s) 1273, 2598, 2619
XspI CTAG 4 cut(s) 284, 885, 1730, 2649
ZrmI AGTACT 3 cut(s) 171, 1228, 2230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.