FvH4_4g30880

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000563
Physical Location & Seq
Reverse (-)
30285589 .. 30286574
986 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30880.t1

Sequence Viewer

Length: 879 bp
ATGTCTGAGTTTGGAGTTGTATATGATTCCTATCCAGAAGGTCGTCCTGGTAGAATGAGACATATAATTGTGGGATCAAAAGTGTATCTGGTTGGCGGTCGCAGACTCGATCAAAGAATCCATAATGCTGCAGATGGGAGATGTTTTCCAATTCCCAACTTAGATATGTATGCACTCAATTGCGATTATGAAACAGAAGAGGGTCCTTCTATTACCCTCTGCCAATGTGAAGAAGAACTTAATGATCATGATCAGCCACCACTTTTTAATGTTTTTGATACGCTCAAACAATCTTGGAAACAACTCCCTCATCCTCCCTGCTATGATATATCATCTAGCGACACTGAGCCTTATGTGTGTAACTACTTCTCTTGGGGCAACAAGCTCATTATTCGTACCGAGACAAAGAGGAAGAAGAAGAAGAAGAAGAAGCGCGGCACGTACGTTTTTGACACTCAACAAGAGAGATGGGAGGATGCCAAGAATATGAGTGCAATCCTTGATGAGGGGTTCAGGGTTGTGGTAGAGTTCCAAGGATTCTTGATTGGCATGCCTTATAACCGCCAAGAAATTGTTGCTTACAGTTTGGATTCGGATGGGATTCCCAAACTCTCTAAGGTGCTACACGAGCTGCAAGGGATATTTAGCTCATGTTCACTTGTCTTCGACTGTTTTCTTGGTAAATATGACGGTGACCGTATGTGGCTGGTGTATTCTGCGCAGGATAGTTTGAAAGTATGGCATACGCGTGTAGCGGTGTTTCGTGTTTTCATCTCTAATGACTTGGATGGAAATGAAGTCCTCTGTGCACATCTTGAGGCGGCAGAATTCTTTCAGTTCGATAGAATTATTAGCTCAGCTTTCACCTATGCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

33.72

Weight (kDa)

5.57

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 55 - 161 8.2e-08 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
Acc16I TGCGCA 1 cut(s) 720
AccII CGCG 2 cut(s) 435, 748
AciI CCGC 5 cut(s) 96, 435, 562, 755, 821
AclWI GGATC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 827
AfaI GTAC 2 cut(s) 397, 443
AfiI CCNNNNNNNGG 1 cut(s) 505
AflIII ACRYGT 1 cut(s) 746
AgsI TTSAA 1 cut(s) 733
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 5 cut(s) 385, 631, 648, 855, 860
AluI AGCT 5 cut(s) 385, 631, 648, 855, 860
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 2 cut(s) 52, 395
Alw44I GTGCAC 1 cut(s) 807
AlwI GGATC 1 cut(s) 82
ApaLI GTGCAC 1 cut(s) 807
ApeKI GCWGC 2 cut(s) 128, 631
ApoI RAATTY 1 cut(s) 827
Asp700I GAANNNNTTC 1 cut(s) 831
AspLEI GCGC 2 cut(s) 435, 721
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 704, 856
AvaII GGWCC 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 811
BauI CACGAG 1 cut(s) 626
BbsI GAAGAC 1 cut(s) 655
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 2 cut(s) 115, 618
BccI CCATC 4 cut(s) 128, 462, 590, 782
BciT130I CCWGG 1 cut(s) 48
BclI TGATCA 2 cut(s) 244, 250
BcoDI GTCTC 2 cut(s) 52, 395
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 129
BisI GCNGC 4 cut(s) 129, 436, 632, 822
BlpI GCTNAGC 1 cut(s) 856
BlsI GCNGC 4 cut(s) 130, 437, 633, 823
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 655
Bpu1102I GCTNAGC 1 cut(s) 856
BpuEI CTTGAG 1 cut(s) 836
BsaAI YACGTR 1 cut(s) 441
BsaBI GATNNNNATC 2 cut(s) 30, 249
BsaJI CCNNGG 1 cut(s) 532
BsaXI ACNNNNNCTCC 1 cut(s) 856
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse8I GATNNNNATC 2 cut(s) 30, 249
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 4 cut(s) 310, 481, 601, 793
BseJI GATNNNNATC 2 cut(s) 30, 249
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMII CTCAG 2 cut(s) 336, 870
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 2 cut(s) 115, 618
Bsh1236I CGCG 2 cut(s) 435, 748
Bsh1285I CGRYCG 1 cut(s) 100
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 811
BsiWI CGTACG 1 cut(s) 441
BslI CCNNNNNNNGG 1 cut(s) 505
BsmAI GTCTC 2 cut(s) 52, 395
Bsp1286I GDGCHC 1 cut(s) 811
Bsp143I GATC 4 cut(s) 74, 109, 244, 250
Bsp1720I GCTNAGC 1 cut(s) 856
BspACI CCGC 5 cut(s) 96, 435, 562, 755, 821
BspCNI CTCAG 2 cut(s) 337, 869
BspFNI CGCG 2 cut(s) 435, 748
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 4 cut(s) 74, 109, 244, 250
BssSI CACGAG 1 cut(s) 626
BssT1I CCWWGG 1 cut(s) 532
Bst2BI CACGAG 1 cut(s) 626
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 4 cut(s) 584, 671, 692, 698
Bst6I CTCTTC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 551
BstDEI CTNAG 5 cut(s) 6, 160, 345, 615, 856
BstEII GGTNACC 1 cut(s) 692
BstENI CCTNNNNNAGG 1 cut(s) 503
BstF5I GGATG 4 cut(s) 310, 481, 601, 793
BstFNI CGCG 2 cut(s) 435, 748
BstHHI GCGC 2 cut(s) 435, 721
BstKTI GATC 4 cut(s) 77, 112, 247, 253
BstMAI GTCTC 2 cut(s) 52, 395
BstMBI GATC 4 cut(s) 74, 109, 244, 250
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 1 cut(s) 628
BstNI CCWGG 1 cut(s) 48
BstNSI RCATGY 1 cut(s) 553
BstPI GGTNACC 1 cut(s) 692
BstSCI CCNGG 1 cut(s) 46
BstSFI CTRYAG 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 2 cut(s) 435, 748
BstV1I GCAGC 2 cut(s) 115, 618
BstV2I GAAGAC 1 cut(s) 655
BtsCI GGATG 4 cut(s) 310, 481, 601, 793
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 551
CciI TCATGA 1 cut(s) 247
CfoI GCGC 2 cut(s) 435, 721
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 396, 442
CviAII CATG 4 cut(s) 248, 550, 651, 876
CviJI RGCY 8 cut(s) 256, 349, 385, 631, 648, 706, 855, 860
CviKI_1 RGCY 8 cut(s) 256, 349, 385, 631, 648, 706, 855, 860
CviQI GTAC 2 cut(s) 396, 442
DdeI CTNAG 5 cut(s) 6, 160, 345, 615, 856
DpnI GATC 4 cut(s) 76, 111, 246, 252
DpnII GATC 4 cut(s) 74, 109, 244, 250
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 692
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 203
EcoO65I GGTNACC 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 4 cut(s) 251, 553, 654, 879
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FatI CATG 4 cut(s) 247, 549, 650, 875
FbaI TGATCA 2 cut(s) 244, 250
Fnu4HI GCNGC 4 cut(s) 129, 436, 632, 822
FokI GGATG 4 cut(s) 297, 488, 608, 800
Fsp4HI GCNGC 4 cut(s) 129, 436, 632, 822
FspBI CTAG 1 cut(s) 336
FspI TGCGCA 1 cut(s) 720
GlaI GCGC 2 cut(s) 434, 720
GluI GCNGC 4 cut(s) 129, 436, 632, 822
HhaI GCGC 2 cut(s) 435, 721
Hin1II CATG 4 cut(s) 251, 553, 654, 879
Hin6I GCGC 2 cut(s) 433, 719
HinP1I GCGC 2 cut(s) 433, 719
HinfI GANTC 6 cut(s) 26, 105, 117, 537, 590, 601
HphI GGTGA 2 cut(s) 704, 856
Hpy166II GTNNAC 2 cut(s) 656, 809
Hpy188I TCNGA 2 cut(s) 7, 595
Hpy188III TCNNGA 4 cut(s) 35, 248, 541, 815
Hpy8I GTNNAC 2 cut(s) 656, 809
HpyAV CCTTC 2 cut(s) 32, 216
HpyCH4III ACNGT 4 cut(s) 584, 671, 692, 698
HpyCH4IV ACGT 2 cut(s) 440, 444
HpyCH4V TGCA 5 cut(s) 131, 173, 494, 634, 809
HpyF10VI GCNNNNNNNGC 1 cut(s) 628
HpyF3I CTNAG 5 cut(s) 6, 160, 345, 615, 856
HpySE526I ACGT 2 cut(s) 440, 444
Hsp92II CATG 4 cut(s) 251, 553, 654, 879
HspAI GCGC 2 cut(s) 433, 719
Ksp22I TGATCA 2 cut(s) 244, 250
Kzo9I GATC 4 cut(s) 74, 109, 244, 250
LmnI GCTCC 1 cut(s) 877
LpnPI CCDG 8 cut(s) 33, 48, 60, 74, 331, 499, 692, 707
Lsp1109I GCAGC 2 cut(s) 115, 618
LweI GCATC 1 cut(s) 466
MaeI CTAG 1 cut(s) 336
MaeII ACGT 2 cut(s) 440, 444
MaeIII GTNAC 2 cut(s) 359, 692
MalI GATC 4 cut(s) 76, 111, 246, 252
MboI GATC 4 cut(s) 74, 109, 244, 250
MfeI CAATTG 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 811
MluCI AATT 6 cut(s) 66, 150, 178, 570, 827, 846
MluI ACGCGT 1 cut(s) 746
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 9 cut(s) 193, 227, 318, 324, 402, 466, 499, 811, 812
MroXI GAANNNNTTC 1 cut(s) 831
MseI TTAA 2 cut(s) 240, 267
MslI CAYNNNNRTG 1 cut(s) 747
MspR9I CCNGG 1 cut(s) 48
MunI CAATTG 1 cut(s) 178
MvaI CCWGG 1 cut(s) 48
MvnI CGCG 2 cut(s) 435, 748
MwoI GCNNNNNNNGC 1 cut(s) 628
NdeII GATC 4 cut(s) 74, 109, 244, 250
NlaIII CATG 4 cut(s) 251, 553, 654, 879
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 692
NsbI TGCGCA 1 cut(s) 720
NspI RCATGY 1 cut(s) 553
PaeI GCATGC 1 cut(s) 553
PagI TCATGA 1 cut(s) 247
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 5 cut(s) 26, 117, 537, 590, 601
Pfl23II CGTACG 1 cut(s) 441
PkrI GCNGC 4 cut(s) 130, 437, 633, 823
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
Ppu21I YACGTR 1 cut(s) 441
PpuMI RGGWCCY 1 cut(s) 203
PsiI TTATAA 1 cut(s) 558
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 46
PspEI GGTNACC 1 cut(s) 692
PspGI CCWGG 1 cut(s) 46
PspLI CGTACG 1 cut(s) 441
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 203
PspPPI RGGWCCY 1 cut(s) 203
PstI CTGCAG 1 cut(s) 133
RsaI GTAC 2 cut(s) 397, 443
RsaNI GTAC 2 cut(s) 396, 442
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 2 cut(s) 240, 267
SatI GCNGC 4 cut(s) 129, 436, 632, 822
Sau3AI GATC 4 cut(s) 74, 109, 244, 250
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 1 cut(s) 811
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SphI GCATGC 1 cut(s) 553
Sse9I AATT 6 cut(s) 66, 150, 178, 570, 827, 846
SsiI CCGC 5 cut(s) 96, 435, 562, 755, 821
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 46
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 4 cut(s) 584, 671, 692, 698
TaiI ACGT 2 cut(s) 443, 447
TaqI TCGA 3 cut(s) 108, 666, 840
TasI AATT 6 cut(s) 66, 150, 178, 570, 827, 846
TauI GCSGC 2 cut(s) 438, 824
TfiI GAWTC 5 cut(s) 26, 117, 537, 590, 601
Tru1I TTAA 2 cut(s) 240, 267
Tru9I TTAA 2 cut(s) 240, 267
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 2 cut(s) 128, 631
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 3 cut(s) 204, 760, 810
TspRI CASTG 1 cut(s) 349
VneI GTGCAC 1 cut(s) 807
VpaK11BI GGWCC 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 1 cut(s) 827
XceI RCATGY 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.