Rh5AG494400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000563
Physical Location & Seq
Reverse (-)
84121220 .. 84122062
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG494400.1

Sequence Viewer

Length: 843 bp
ATGGTTGGGGGCGAAAGAGTGGATCGAAACCTGCATTGTGATGCCAATGGCAGATGTTTTCCTATGCCGAACTTGGATATTTATTCCTTTAATCCCACTCTCCCAAACCGTCCATTGATCACCATGTGCAACGGTGTCATTCCCATGTTGATTGATCCAAAAGCTAAGACCATTCCTATCGTGACCACTATTGAGGAGAAAATTTATGTTCTCACCAAAAGACCATATTCAGAAGAACTTGATGATGATCAGCGCCCCAAATTTAAGGTTTTTGATCCCCTCCACCAATCTTGGAAACATCTTCCTGATCCCCCTTGTTATAATAAATCATTACATGACACAGAACTTGTTGTGCATAACCACTTTGCTTGGGGGCACAAGCTTATTATTAGCACCAAGTTGGGTTCGTACATTTTTGATACTCATCAAGAGAAATGGGAGGATACCCCCCACGAATTCCCGAATGAGGGGTTTAAGGCTGTTGTAGAAGTTCGGGGATTTTTAATTGGAATTCCTCATAATGGCAAAGAGCTTGTTGCTTATCAGTTGGATTCAAATGGTATCTTCAAGCCATATAAGGTGTTACATGAACTGCAGGGAATATTCCGTACTTCTTCACTTGTTTTTGACTATTTCCTAGGTAAATGTGATGATAAACGTATATGGTTGGTGTATTCTGGCATGGATGGTCCAAATTATTGGAATACATGGGTAGTAGTGTTGAGAGTTTTCACCTCAATCGACAATGTTGGCAATTCAGTCCTCTCAGTAGACATCGAGGCTGCAGGGTGTTTGCATAGTTTACCGATCAGTTCAGCTTTCTCATTGTGTCATCTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

32.0

Weight (kDa)

6.1

Isoelectric Point (pI)

27.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 53 - 194 9.5e-12 Protein of unknown function (DUF1668)
Kelch_FKB95 PF25210 57 - 215 8.5e-09 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 321
Acc36I ACCTGC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 771
AclWI GGATC 4 cut(s) 30, 149, 269, 302
AcsI RAATTY 4 cut(s) 201, 260, 455, 510
AdeI CACNNNGTG 1 cut(s) 126
AfaI GTAC 2 cut(s) 410, 610
AfiI CCNNNNNNNGG 3 cut(s) 466, 467, 521
AgsI TTSAA 2 cut(s) 555, 568
AluBI AGCT 4 cut(s) 164, 382, 532, 818
AluI AGCT 4 cut(s) 164, 382, 532, 818
AlwI GGATC 4 cut(s) 30, 149, 269, 302
ApeKI GCWGC 1 cut(s) 782
ApoI RAATTY 4 cut(s) 201, 260, 455, 510
AspA2I CCTAGG 1 cut(s) 637
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 689
AsuHPI GGTGA 3 cut(s) 112, 205, 724
AvaII GGWCC 1 cut(s) 689
AvrII CCTAGG 1 cut(s) 637
BaeGI GKGCMC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 769
BccI CCATC 1 cut(s) 680
BciVI GTATCC 1 cut(s) 436
BclI TGATCA 2 cut(s) 117, 247
BfaI CTAG 1 cut(s) 638
BfmI CTRYAG 2 cut(s) 593, 783
BfoI RGCGCY 1 cut(s) 256
BfuAI ACCTGC 1 cut(s) 39
BfuI GTATCC 1 cut(s) 436
BisI GCNGC 1 cut(s) 783
BlnI CCTAGG 1 cut(s) 637
BlsI GCNGC 1 cut(s) 784
Bme18I GGWCC 1 cut(s) 689
BmgT120I GGNCC 1 cut(s) 689
BmsI GCATC 1 cut(s) 31
BsaBI GATNNNNATC 2 cut(s) 246, 423
BsaJI CCNNGG 1 cut(s) 637
Bsc4I CCNNNNNNNGG 3 cut(s) 466, 467, 521
Bse8I GATNNNNATC 2 cut(s) 246, 423
BseDI CCNNGG 1 cut(s) 637
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 2 cut(s) 246, 423
BseLI CCNNNNNNNGG 3 cut(s) 466, 467, 521
BseMII CTCAG 1 cut(s) 780
BseRI GAGGAG 1 cut(s) 209
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 1 cut(s) 769
BslI CCNNNNNNNGG 3 cut(s) 466, 467, 521
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
BspCNI CTCAG 1 cut(s) 779
BspMAI CTGCAG 2 cut(s) 597, 787
BspMI ACCTGC 1 cut(s) 39
BspPI GGATC 4 cut(s) 30, 149, 269, 302
BssECI CCNNGG 1 cut(s) 637
BssMI GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
BssT1I CCWWGG 1 cut(s) 637
Bst4CI ACNGT 2 cut(s) 110, 134
BstDEI CTNAG 2 cut(s) 165, 766
BstF5I GGATG 1 cut(s) 691
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 7 cut(s) 25, 120, 157, 250, 277, 310, 810
BstMBI GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
BstSFI CTRYAG 2 cut(s) 593, 783
BstSLI GKGCMC 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 769
BstXI CCANNNNNNTGG 1 cut(s) 699
BsuI GTATCC 1 cut(s) 436
BtsCI GGATG 1 cut(s) 691
BveI ACCTGC 1 cut(s) 39
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 689
Csp6I GTAC 2 cut(s) 409, 609
CspCI CAANNNNNGTGG 4 cut(s) 272, 307, 350, 385
CviAII CATG 6 cut(s) 124, 145, 335, 587, 682, 708
CviJI RGCY 7 cut(s) 164, 382, 479, 532, 571, 782, 818
CviKI_1 RGCY 7 cut(s) 164, 382, 479, 532, 571, 782, 818
CviQI GTAC 2 cut(s) 409, 609
DdeI CTNAG 2 cut(s) 165, 766
DpnI GATC 7 cut(s) 24, 119, 156, 249, 276, 309, 809
DpnII GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
DraIII CACNNNGTG 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 689
EcoRI GAATTC 2 cut(s) 455, 510
EcoT14I CCWWGG 1 cut(s) 637
ErhI CCWWGG 1 cut(s) 637
FaeI CATG 6 cut(s) 127, 148, 338, 590, 685, 711
FatI CATG 6 cut(s) 123, 144, 334, 586, 681, 707
FbaI TGATCA 2 cut(s) 117, 247
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 1 cut(s) 783
FokI GGATG 1 cut(s) 698
Fsp4HI GCNGC 1 cut(s) 783
FspBI CTAG 1 cut(s) 638
GlaI GCGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 783
HaeII RGCGCY 1 cut(s) 256
HhaI GCGC 1 cut(s) 255
Hin1II CATG 6 cut(s) 127, 148, 338, 590, 685, 711
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 551
HphI GGTGA 3 cut(s) 112, 205, 724
Hpy166II GTNNAC 2 cut(s) 772, 803
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 4 cut(s) 181, 305, 428, 460
Hpy8I GTNNAC 2 cut(s) 772, 803
HpyCH4III ACNGT 2 cut(s) 110, 134
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 6 cut(s) 34, 129, 355, 595, 785, 796
HpyF3I CTNAG 2 cut(s) 165, 766
HpySE526I ACGT 1 cut(s) 658
Hsp92II CATG 6 cut(s) 127, 148, 338, 590, 685, 711
HspAI GCGC 1 cut(s) 253
Ksp22I TGATCA 2 cut(s) 117, 247
Kzo9I GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
LpnPI CCDG 5 cut(s) 44, 318, 581, 663, 771
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 1 cut(s) 31
MaeI CTAG 1 cut(s) 638
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 2 cut(s) 181, 582
MalI GATC 7 cut(s) 24, 119, 156, 249, 276, 309, 809
MboI GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
MboII GAAGA 4 cut(s) 245, 293, 556, 606
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 7 cut(s) 201, 260, 455, 504, 510, 694, 754
MmeI TCCRAC 1 cut(s) 528
MnlI CCTC 8 cut(s) 187, 290, 433, 460, 525, 745, 772, 773
MseI TTAA 4 cut(s) 90, 264, 474, 503
MslI CAYNNNNRTG 2 cut(s) 39, 143
NdeII GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
NlaIII CATG 6 cut(s) 127, 148, 338, 590, 685, 711
NmuCI GTSAC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 551
PkrI GCNGC 1 cut(s) 784
PsiI TTATAA 1 cut(s) 321
PspPI GGNCC 1 cut(s) 689
PstI CTGCAG 2 cut(s) 597, 787
RsaI GTAC 2 cut(s) 410, 610
RsaNI GTAC 2 cut(s) 409, 609
RseI CAYNNNNRTG 2 cut(s) 39, 143
SaqAI TTAA 4 cut(s) 90, 264, 474, 503
SatI GCNGC 1 cut(s) 783
Sau3AI GATC 7 cut(s) 22, 117, 154, 247, 274, 307, 807
Sau96I GGNCC 1 cut(s) 689
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 2 cut(s) 593, 783
SinI GGWCC 1 cut(s) 689
SmiMI CAYNNNNRTG 2 cut(s) 39, 143
Sse9I AATT 7 cut(s) 201, 260, 455, 504, 510, 694, 754
SspI AATATT 1 cut(s) 603
SspMI CTAG 1 cut(s) 638
StyI CCWWGG 1 cut(s) 637
TaaI ACNGT 2 cut(s) 110, 134
TaiI ACGT 1 cut(s) 661
TaqI TCGA 3 cut(s) 25, 741, 777
TasI AATT 7 cut(s) 201, 260, 455, 504, 510, 694, 754
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 4 cut(s) 90, 264, 474, 503
Tru9I TTAA 4 cut(s) 90, 264, 474, 503
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 782
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 603
TspGWI ACGGA 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 4 cut(s) 201, 260, 455, 510
XmaJI CCTAGG 1 cut(s) 637
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.