pycom15g03830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000563
Physical Location & Seq
Forward (+)
2338330 .. 2339589
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03830.1

Sequence Viewer

Length: 636 bp
ATGTCCCGCTGTGCTATTGGCCGCAAGCTGTATCTTAATACCAGCCATTTTTCATGCATTTTTGATGCAGAAAAAGAAGAATGGGACTATGCCATCCCATTGAAGTTTCCCTATCTTATGTGCTCTGTAGAGTACAATGGCTTTCTAATTGCTATGCCCTTATTCGGCGAAGCAGAAGTTGTTGCCTATAACTTGGACTCATTTGGGCTCACCAATTCTGAAAAGCGTCTAGATCAGCTACAGGAAATATTCAAGGATCCCTTTTACGACCCCATAGAGTGCTATCTCACCAGTCTTGACGATCATGGCCGCATGTGCCTCCTTTATTCAGGTTTCTCCTATTCCAGCTCTTCCAAAGAGTTTTGGGGACGTGTAGCGGTGTTTCAAGTATCTGTCTCAAACTCTACGGACAATCCAGTTGTCACTGCAGTTCTAGAGGCTGTCGAAGACTATAACATGCAGGACTTTGTAGTTTGTGATACCAGTATTTACTCAACATTCATCATGTATGATGATCTGAGCATGGATGATACCGAGGCTGATGCAAGTTTAACGAACTCAACTCTTTCTGGCTTGGATGCTGTTATGTATGAAGAACCGGAAATTACAGTTTCTGAAACAGGCCAGTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.99

Weight (kDa)

4.05

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 7, 22, 310, 377
AclWI GGATC 2 cut(s) 251, 264
AcoI YGGCCR 2 cut(s) 19, 307
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 164
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 103, 253, 386
AjiI CACGTC 1 cut(s) 371
AluBI AGCT 3 cut(s) 28, 238, 348
AluI AGCT 3 cut(s) 28, 238, 348
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 2 cut(s) 251, 264
AlwNI CAGNNNCTG 1 cut(s) 614
AoxI GGCC 3 cut(s) 19, 307, 622
AsuHPI GGTGA 2 cut(s) 202, 280
BamHI GGATCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 125
BccI CCATC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 2 cut(s) 230, 434
BfmI CTRYAG 3 cut(s) 126, 239, 426
BisI GCNGC 2 cut(s) 22, 310
BlsI GCNGC 2 cut(s) 23, 311
BmgBI CACGTC 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 258
BmsI GCATC 3 cut(s) 55, 532, 568
BpiI GAAGAC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 534
BsaWI WCCGGW 1 cut(s) 598
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 4 cut(s) 291, 416, 483, 625
BseDI CCNNGG 1 cut(s) 534
BseGI GGATG 3 cut(s) 93, 532, 583
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 1 cut(s) 509
BseNI ACTGG 4 cut(s) 291, 416, 483, 625
BshFI GGCC 3 cut(s) 21, 309, 624
BsiHKAI GWGCWC 1 cut(s) 125
BsiSI CCGG 1 cut(s) 599
BslFI GGGAC 2 cut(s) 98, 381
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 400
BsmFI GGGAC 2 cut(s) 98, 381
BsnI GGCC 3 cut(s) 21, 309, 624
Bsp1286I GDGCHC 2 cut(s) 125, 210
Bsp143I GATC 4 cut(s) 232, 256, 301, 514
BspACI CCGC 4 cut(s) 7, 22, 310, 377
BspANI GGCC 3 cut(s) 21, 309, 624
BspCNI CTCAG 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 258
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 2 cut(s) 251, 264
BspQI GCTCTTC 1 cut(s) 355
BsrI ACTGG 4 cut(s) 291, 416, 483, 625
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 4 cut(s) 232, 256, 301, 514
Bst4CI ACNGT 1 cut(s) 610
Bst6I CTCTTC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 1 cut(s) 518
BstF5I GGATG 3 cut(s) 93, 532, 583
BstKTI GATC 4 cut(s) 235, 259, 304, 517
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 4 cut(s) 232, 256, 301, 514
BstMWI GCNNNNNNNGC 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 316, 460
BstSFI CTRYAG 3 cut(s) 126, 239, 426
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 3 cut(s) 21, 309, 624
BtrI CACGTC 1 cut(s) 371
BtsCI GGATG 3 cut(s) 93, 532, 583
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 1 cut(s) 26
CaiI CAGNNNCTG 1 cut(s) 614
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 133
CviAII CATG 6 cut(s) 54, 305, 313, 457, 505, 523
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 518
DpnI GATC 4 cut(s) 234, 258, 303, 516
DpnII GATC 4 cut(s) 232, 256, 301, 514
EaeI YGGCCR 2 cut(s) 19, 307
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
Eco24I GRGCYC 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 6 cut(s) 57, 308, 316, 460, 508, 526
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 2 cut(s) 98, 381
FatI CATG 6 cut(s) 53, 304, 312, 456, 504, 522
FauI CCCGC 1 cut(s) 14
Fnu4HI GCNGC 2 cut(s) 22, 310
FokI GGATG 3 cut(s) 80, 539, 590
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 2 cut(s) 22, 310
FspBI CTAG 2 cut(s) 230, 434
GluI GCNGC 2 cut(s) 22, 310
HaeIII GGCC 3 cut(s) 21, 309, 624
HapII CCGG 1 cut(s) 599
HgaI GACGC 1 cut(s) 215
Hin1II CATG 6 cut(s) 57, 308, 316, 460, 508, 526
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 599
HphI GGTGA 2 cut(s) 202, 280
Hpy188I TCNGA 3 cut(s) 220, 519, 616
Hpy188III TCNNGA 3 cut(s) 230, 296, 434
HpyCH4III ACNGT 1 cut(s) 610
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 5 cut(s) 57, 68, 428, 460, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 315
HpyF3I CTNAG 1 cut(s) 518
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 6 cut(s) 57, 308, 316, 460, 508, 526
Kzo9I GATC 4 cut(s) 232, 256, 301, 514
LguI GCTCTTC 1 cut(s) 355
LweI GCATC 3 cut(s) 55, 532, 568
MaeI CTAG 2 cut(s) 230, 434
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 1 cut(s) 421
MalI GATC 4 cut(s) 234, 258, 303, 516
MboI GATC 4 cut(s) 232, 256, 301, 514
MboII GAAGA 4 cut(s) 89, 342, 458, 605
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 125, 210
MluCI AATT 3 cut(s) 147, 214, 603
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 3 cut(s) 329, 430, 529
Mph1103I ATGCAT 1 cut(s) 59
MseI TTAA 2 cut(s) 36, 551
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 1 cut(s) 599
MwoI GCNNNNNNNGC 1 cut(s) 315
NdeII GATC 4 cut(s) 232, 256, 301, 514
NlaIII CATG 6 cut(s) 57, 308, 316, 460, 508, 526
NlaIV GGNNCC 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 59
NspI RCATGY 2 cut(s) 316, 460
PciSI GCTCTTC 1 cut(s) 355
PkrI GCNGC 2 cut(s) 23, 311
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PspN4I GGNNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 430
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 355
SaqAI TTAA 2 cut(s) 36, 551
SatI GCNGC 2 cut(s) 22, 310
Sau3AI GATC 4 cut(s) 232, 256, 301, 514
SchI GAGTC 1 cut(s) 191
SduI GDGCHC 2 cut(s) 125, 210
SetI ASST 5 cut(s) 30, 240, 334, 350, 373
SfaNI GCATC 3 cut(s) 55, 532, 568
SfcI CTRYAG 3 cut(s) 126, 239, 426
Sse9I AATT 3 cut(s) 147, 214, 603
SsiI CCGC 4 cut(s) 7, 22, 310, 377
SspI AATATT 1 cut(s) 249
SspMI CTAG 2 cut(s) 230, 434
TaaI ACNGT 1 cut(s) 610
TaiI ACGT 1 cut(s) 373
TaqI TCGA 1 cut(s) 444
TasI AATT 3 cut(s) 147, 214, 603
TatI WGTACW 1 cut(s) 132
TauI GCSGC 2 cut(s) 24, 312
Tru1I TTAA 2 cut(s) 36, 551
Tru9I TTAA 2 cut(s) 36, 551
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 3 cut(s) 42, 490, 606
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 1 cut(s) 430
XbaI TCTAGA 2 cut(s) 229, 433
XceI RCATGY 2 cut(s) 316, 460
XspI CTAG 2 cut(s) 230, 434
Zsp2I ATGCAT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.