FvH4_6g30071

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000563
Physical Location & Seq
Reverse (-)
23228488 .. 23229184
697 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30071.t1

Sequence Viewer

Length: 459 bp
ATGTGTTTAGGTGACATACCATACCACATAGGATTAAGGTCACAAATCTTCGGCCTTATTAATGCCAAGAATCATTTCCTTTCATTAAATTGCAAACAAGTTCAAACAAATTACAAAGTACGAAATGTGGAAAGTCAGAGAGAGGCAAACACAATTTTCGACCGCGCCTGTCCGGCTGTGCTCGCCGCCAGCGACGGTTTGGCTAGAGAGACTTGGTCTCTAATTTGGGCTCTCGAAGTTTCTGACGAGAGGAGAGGGGCAATGCCAGTCGATGGAGTGTCATGGATGGTCGGCGATGACAACTTGACAGACGGCGACAATGTCTTCTGCTGGTCTGGCAATGTGTGGTTAGGAAGGGTGATGGTGAGGCAATGGTTTTGGGCTGGGAGAGTTGGGCTTCATCCAACTGATCGATCACCTTTTCAAGTTTGGGCTTTTGGTGGGCAGAAGTTGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.11

Weight (kDa)

6.81

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 272
AccII CGCG 1 cut(s) 165
AciI CCGC 2 cut(s) 163, 186
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 2 cut(s) 104, 425
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 2 cut(s) 203, 222
AoxI GGCC 1 cut(s) 52
AseI ATTAAT 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 167
AsuHPI GGTGA 4 cut(s) 23, 370, 376, 408
BanII GRGCYC 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 316
Bbv12I GWGCWC 1 cut(s) 183
BccI CCATC 3 cut(s) 266, 280, 355
BceAI ACGGC 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 203, 222
BfaI CTAG 2 cut(s) 204, 457
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 316
Bsa29I ATCGAT 1 cut(s) 412
BsaI GGTCTC 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 3 cut(s) 267, 346, 377
BseCI ATCGAT 1 cut(s) 412
BseGI GGATG 2 cut(s) 291, 400
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMI GCAATG 3 cut(s) 267, 346, 377
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 1 cut(s) 265
BseYI CCCAGC 1 cut(s) 383
Bsh1236I CGCG 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 54
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 2 cut(s) 203, 222
BsnI GGCC 1 cut(s) 54
Bso31I GGTCTC 1 cut(s) 222
Bsp1286I GDGCHC 2 cut(s) 183, 232
Bsp143I GATC 2 cut(s) 409, 413
BspACI CCGC 2 cut(s) 163, 186
BspANI GGCC 1 cut(s) 54
BspDI ATCGAT 1 cut(s) 412
BspFNI CGCG 1 cut(s) 165
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 3 cut(s) 267, 346, 377
BsrI ACTGG 1 cut(s) 266
BssMI GATC 2 cut(s) 409, 413
Bst4CI ACNGT 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 183, 190
BstF5I GGATG 2 cut(s) 291, 400
BstFNI CGCG 1 cut(s) 165
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 412, 416
BstMAI GTCTC 2 cut(s) 203, 222
BstMBI GATC 2 cut(s) 409, 413
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 3 cut(s) 173, 182, 336
BstUI CGCG 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 412
BsuRI GGCC 1 cut(s) 54
BsuTUI ATCGAT 1 cut(s) 412
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 2 cut(s) 291, 400
Cac8I GCNNGC 2 cut(s) 183, 190
CfoI GCGC 1 cut(s) 167
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 1 cut(s) 119
CviAII CATG 1 cut(s) 282
CviJI RGCY 8 cut(s) 54, 176, 203, 230, 383, 397, 434, 456
CviKI_1 RGCY 8 cut(s) 54, 176, 203, 230, 383, 397, 434, 456
CviQI GTAC 1 cut(s) 119
DpnI GATC 2 cut(s) 411, 415
DpnII GATC 2 cut(s) 409, 413
Eco24I GRGCYC 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 232
FaeI CATG 1 cut(s) 285
FaiI YATR 4 cut(s) 17, 22, 29, 283
FatI CATG 1 cut(s) 281
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 2 cut(s) 298, 387
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 2 cut(s) 204, 457
GlaI GCGC 1 cut(s) 166
GluI GCNGC 1 cut(s) 186
GsaI CCCAGC 1 cut(s) 387
HaeIII GGCC 1 cut(s) 54
HapII CCGG 1 cut(s) 173
HhaI GCGC 1 cut(s) 167
Hin1II CATG 1 cut(s) 285
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HinfI GANTC 1 cut(s) 70
HpaII CCGG 1 cut(s) 173
HphI GGTGA 4 cut(s) 23, 370, 376, 408
Hpy188I TCNGA 2 cut(s) 138, 244
Hpy188III TCNNGA 1 cut(s) 233
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 348
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 1 cut(s) 93
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 182, 336
Hsp92II CATG 1 cut(s) 285
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 409, 413
LpnPI CCDG 7 cut(s) 181, 186, 202, 279, 316, 321, 369
MaeI CTAG 2 cut(s) 204, 457
MaeIII GTNAC 2 cut(s) 11, 39
MalI GATC 2 cut(s) 411, 415
MboI GATC 2 cut(s) 409, 413
MboII GAAGA 2 cut(s) 40, 316
MhlI GDGCHC 2 cut(s) 183, 232
MluCI AATT 4 cut(s) 88, 109, 153, 222
MmeI TCCRAC 1 cut(s) 428
MnlI CCTC 4 cut(s) 136, 243, 248, 360
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 3 cut(s) 35, 60, 86
MspI CCGG 1 cut(s) 173
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 3 cut(s) 173, 182, 336
NdeII GATC 2 cut(s) 409, 413
NlaIII CATG 1 cut(s) 285
NmuCI GTSAC 2 cut(s) 11, 39
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 70
PflFI GACNNNGTC 2 cut(s) 214, 320
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 1 cut(s) 187
PshBI ATTAAT 1 cut(s) 60
PspFI CCCAGC 1 cut(s) 383
PsyI GACNNNGTC 2 cut(s) 214, 320
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
SaqAI TTAA 3 cut(s) 35, 60, 86
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 409, 413
SduI GDGCHC 2 cut(s) 183, 232
SetI ASST 3 cut(s) 13, 41, 421
Sse9I AATT 4 cut(s) 88, 109, 153, 222
SsiI CCGC 2 cut(s) 163, 186
SspMI CTAG 2 cut(s) 204, 457
TaaI ACNGT 1 cut(s) 197
TaqI TCGA 4 cut(s) 159, 234, 270, 412
TasI AATT 4 cut(s) 88, 109, 153, 222
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 3 cut(s) 35, 60, 86
Tru9I TTAA 3 cut(s) 35, 60, 86
TseFI GTSAC 2 cut(s) 11, 39
Tsp45I GTSAC 2 cut(s) 11, 39
TspDTI ATGAA 2 cut(s) 72, 389
Tth111I GACNNNGTC 2 cut(s) 214, 320
Van91I CCANNNNNTGG 1 cut(s) 272
VspI ATTAAT 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 2 cut(s) 204, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.