RLG00000021269

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000563
Physical Location & Seq
Reverse (-)
73558915 .. 73559549
635 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021269

Sequence Viewer

Length: 480 bp
ATGGGCGTTTTGATCAGTTGGGCTGGGCCAAAGCAAATACCCATTGCCAAAGCAAATACATGGGATCGACTATGCCGTCTTGATATCAGTAAGCAACTACGTACGTCCGTCTCTCCAAAGCAGCCCAACAACAAGAACTGGTTTCTGTACTTGAATATGCGCAATCGACTATGCCGCCTGAATATTAGGGAATTCTGCGAAAAGGGTTCTTCCAACGAGCCAAACAGGCCAGCTGGGAGTCCATCCTTCCGTCTCTGCGAGGGTCCGATTTTTAGATTGGTTCCACCCAAGATTGATTTTGGTTCTGACCTAATAGTGTTCAGCATTGAGGAGAAAATCTATGTACTTGAACTACCCCTTTTTAGGGTTTTTGATCCGGTGAACCAATCCTGCAAAGACCTTCCTCATCCCCTGAGTCTGAGGCCAGTTCTTTTGCGGCTACAAGCTCTTCATCAGCATTACCAAATTTGTTACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.43

Weight (kDa)

9.44

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 75
Acc16I TGCGCA 1 cut(s) 161
AciI CCGC 2 cut(s) 175, 436
AclWI GGATC 2 cut(s) 72, 368
AcsI RAATTY 2 cut(s) 191, 465
AfaI GTAC 3 cut(s) 103, 149, 345
AfiI CCNNNNNNNGG 2 cut(s) 363, 364
AgsI TTSAA 2 cut(s) 154, 350
AluBI AGCT 2 cut(s) 233, 446
AluI AGCT 2 cut(s) 233, 446
Alw26I GTCTC 2 cut(s) 115, 257
AlwI GGATC 2 cut(s) 72, 368
AoxI GGCC 3 cut(s) 26, 227, 422
ApeKI GCWGC 1 cut(s) 121
ApoI RAATTY 2 cut(s) 191, 465
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 2 cut(s) 26, 263
AsuHPI GGTGA 1 cut(s) 391
AvaII GGWCC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 133
BccI CCATC 1 cut(s) 250
BceAI ACGGC 1 cut(s) 60
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 115, 257
BfaI CTAG 1 cut(s) 478
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 3 cut(s) 122, 175, 437
BlsI GCNGC 3 cut(s) 123, 176, 438
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 26, 263
BmiI GGNNCC 2 cut(s) 264, 282
BsaAI YACGTR 1 cut(s) 101
BsaWI WCCGGW 1 cut(s) 376
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 364
Bse1I ACTGG 2 cut(s) 143, 425
Bse3DI GCAATG 1 cut(s) 42
BseGI GGATG 2 cut(s) 242, 406
BseLI CCNNNNNNNGG 2 cut(s) 363, 364
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 404, 410
BseNI ACTGG 2 cut(s) 143, 425
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 1 cut(s) 133
BseYI CCCAGC 2 cut(s) 23, 233
BshFI GGCC 3 cut(s) 28, 229, 424
BsiSI CCGG 1 cut(s) 377
BsiWI CGTACG 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 363, 364
BsmAI GTCTC 2 cut(s) 115, 257
BsmBI CGTCTC 2 cut(s) 115, 257
BsnI GGCC 3 cut(s) 28, 229, 424
Bsp143I GATC 3 cut(s) 12, 64, 373
BspACI CCGC 2 cut(s) 175, 436
BspANI GGCC 3 cut(s) 28, 229, 424
BspCNI CTCAG 2 cut(s) 405, 411
BspLI GGNNCC 2 cut(s) 264, 282
BspPI GGATC 2 cut(s) 72, 368
BspQI GCTCTTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 2 cut(s) 143, 425
BssMI GATC 3 cut(s) 12, 64, 373
Bst6I CTCTTC 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 413, 419
BstF5I GGATG 2 cut(s) 242, 406
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 3 cut(s) 15, 67, 376
BstMAI GTCTC 2 cut(s) 115, 257
BstMBI GATC 3 cut(s) 12, 64, 373
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstSNI TACGTA 1 cut(s) 101
BstV1I GCAGC 1 cut(s) 133
BsuRI GGCC 3 cut(s) 28, 229, 424
BtsCI GGATG 2 cut(s) 242, 406
Cac8I GCNNGC 1 cut(s) 231
CfoI GCGC 1 cut(s) 162
Cfr13I GGNCC 2 cut(s) 26, 263
Csp6I GTAC 3 cut(s) 102, 148, 344
CviAII CATG 1 cut(s) 60
CviJI RGCY 9 cut(s) 23, 28, 124, 220, 229, 233, 424, 439, 446
CviKI_1 RGCY 9 cut(s) 23, 28, 124, 220, 229, 233, 424, 439, 446
CviQI GTAC 3 cut(s) 102, 148, 344
DdeI CTNAG 2 cut(s) 413, 419
DpnI GATC 3 cut(s) 14, 66, 375
DpnII GATC 3 cut(s) 12, 64, 373
DrdI GACNNNNNNGTC 1 cut(s) 75
DseDI GACNNNNNNGTC 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Eco105I TACGTA 1 cut(s) 101
Eco32I GATATC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 191
EcoRV GATATC 1 cut(s) 85
Esp3I CGTCTC 2 cut(s) 115, 257
FaeI CATG 1 cut(s) 63
FaiI YATR 5 cut(s) 61, 73, 158, 172, 342
FatI CATG 1 cut(s) 59
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 122, 175, 437
FokI GGATG 2 cut(s) 229, 393
Fsp4HI GCNGC 3 cut(s) 122, 175, 437
FspBI CTAG 1 cut(s) 478
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 1 cut(s) 161
GluI GCNGC 3 cut(s) 122, 175, 437
GsaI CCCAGC 2 cut(s) 27, 237
HaeIII GGCC 3 cut(s) 28, 229, 424
HapII CCGG 1 cut(s) 377
HhaI GCGC 1 cut(s) 162
Hin1II CATG 1 cut(s) 63
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 2 cut(s) 238, 415
HpaII CCGG 1 cut(s) 377
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 3 cut(s) 267, 307, 420
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 382
HpyAV CCTTC 2 cut(s) 256, 410
HpyCH4IV ACGT 2 cut(s) 100, 104
HpyCH4V TGCA 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 2 cut(s) 413, 419
HpySE526I ACGT 2 cut(s) 100, 104
Hsp92II CATG 1 cut(s) 63
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 3 cut(s) 12, 64, 373
LguI GCTCTTC 1 cut(s) 453
Lsp1109I GCAGC 1 cut(s) 133
MaeI CTAG 1 cut(s) 478
MaeII ACGT 2 cut(s) 100, 104
MaeIII GTNAC 1 cut(s) 470
MalI GATC 3 cut(s) 14, 66, 375
MboI GATC 3 cut(s) 12, 64, 373
MboII GAAGA 2 cut(s) 201, 440
MluCI AATT 2 cut(s) 191, 465
MlyI GAGTC 2 cut(s) 247, 424
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 4 cut(s) 253, 322, 414, 414
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeII GATC 3 cut(s) 12, 64, 373
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 2 cut(s) 264, 282
NsbI TGCGCA 1 cut(s) 161
PciSI GCTCTTC 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 73
Pfl23II CGTACG 1 cut(s) 101
PkrI GCNGC 3 cut(s) 123, 176, 438
PleI GAGTC 2 cut(s) 246, 423
PpsI GAGTC 2 cut(s) 246, 423
Ppu21I YACGTR 1 cut(s) 101
PspFI CCCAGC 2 cut(s) 23, 233
PspLI CGTACG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 264, 282
PspPI GGNCC 2 cut(s) 26, 263
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 3 cut(s) 103, 149, 345
RsaNI GTAC 3 cut(s) 102, 148, 344
SapI GCTCTTC 1 cut(s) 453
SatI GCNGC 3 cut(s) 122, 175, 437
Sau3AI GATC 3 cut(s) 12, 64, 373
Sau96I GGNCC 2 cut(s) 26, 263
SchI GAGTC 2 cut(s) 247, 424
SetI ASST 6 cut(s) 103, 107, 235, 312, 402, 448
SinI GGWCC 1 cut(s) 263
SnaBI TACGTA 1 cut(s) 101
Sse9I AATT 2 cut(s) 191, 465
SsiI CCGC 2 cut(s) 175, 436
SspI AATATT 1 cut(s) 184
SspMI CTAG 1 cut(s) 478
TaiI ACGT 2 cut(s) 103, 107
TaqI TCGA 2 cut(s) 67, 166
TasI AATT 2 cut(s) 191, 465
TatI WGTACW 2 cut(s) 147, 343
TauI GCSGC 2 cut(s) 177, 439
TseI GCWGC 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 440
TspGWI ACGGA 2 cut(s) 97, 239
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 2 cut(s) 191, 465
XspI CTAG 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.