Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0000563

Physical Location & Seq

Genomic Coordinates Reverse (-)

27532140 .. 27533706

Gene Structure 1567 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008235

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGCCGATGCAAGCACTACCGCCAAAGAAGGATTGGTTTTTGTACTTGATGATGGACGATCGAATATGCCGTCTAAATATTGATGAATTCTGCAAAAGGAACTCTCCAAAGCCTCAGTTTGAAGTTGTGCATGATTATTATCCTAATGGCGACTATCTTCTTGATACAAATATTCCGCAGGGAATGGAACCTATAGTTCTGGGTTCCAAAGTGTATATGTTGGGGGGTGAATCCACAAGAACGTGTAACCCTGAAATGAAGGATTCTGATTCTGATGATGATTCCGGTGGCAGTGGAAGATATAATTCTGAAAATGATTACCGTAATGACCGTGAAAGGGTACGTCGGCATGATGATTCTCGTGTGCCCAATTTGGATGTTTTTGCCTTTGATCCGAATGCTTCTGAGGGTCCAATCATCAGTCTCTGCATCGACCGTACCATTCCAAGGAAGCTAATTGATCCGAGGTTGATTGATCCAAAGACCTTGTCTGGTCCTGAAGTTTTCACCCTGGAGGGCAAAATCTATGTTATGGCCAGAACATCATACTTGGGCGATGAGGAAGATATGGATCATCACCCACCCCTTTTCAAGGTTTTTGATCCCCTTCGCCAAATTTGGAAAGAGCTTCCCCGTCCTCCCCACTGTGACGATAAATCATCAAGTTTATATCGTTACTCTGCTTGGGGTCACAAGATTGTAACTTGCAATTCTTGGTCGGGTTTATACCTTTTTGACGTTCACCAAGAGAAGTGGGAAAAATGGCCAGAGCAAAAGGAGGACGAAGGGAACAACCACCTTGAGTTTAAGGCTGTTGTAGAATTTGAGGGCTTTTTAATTGGTGTAAAATACCATGAGCTCACTGCTTATCAGTTGGATCCGAATGGTATCCCCAAGCTGTTTCGAGACCTAGATGAGCTCCAGGGGATATTCTGTCCTTTTTTACTCGTCGGTAACTGTGTTATGGGTAAGTGTGATGGTGGCCGAATTTGGTTGGTGTATACCGGCAAGGATCTTATGGAATATTGGCATACACGTGTAGCTGTGTTTCAAGTTTCCATCTCCGACGATCGTGATGGCAATCCGGTCCTCTCCACTGAACTCGAGGCTGCGGAGTTTTATTTATTCCAAAACAGTTATGCTAGATTGTCCTCAGCTTTTTCCATGTGCCATCATTCAAACAACCGTGATATTGAGGAGAAAAACTTATGTGGGACAATTCTAGCACATGATCATCCTCGAGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006479 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016278 GO:0016279 GO:0016740 GO:0016741 GO:0018022 GO:0018023 GO:0018026 GO:0018193 GO:0018205 GO:0019538 GO:0032259 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rubis-subs-bind SET

Protein Analysis

416

Amino Acids

47.98

Weight (kDa)

5.09

Isoelectric Point (pI)

32.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kelch_FKB95	PF25210	165 - 337	5.1e-08	FKB95, Kelch-repeats domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000563)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g40701 FvH4_3g40701 FvH4_3g42210 FvH4_4g30821 FvH4_4g30830 FvH4_4g30861 FvH4_4g30870 FvH4_4g30880 FvH4_4g30890 FvH4_6g30071 FvH4_6g44331 FvH4_6g44332 FvH4_6g44333
malus_domestica	MD15G1041200.v1.1 MD15G1041700.v1.1 MD15G1401000.v1.1
prunus_persica	Prupe.5G008800_v2.0.a1 Prupe.5G157900_v2.0.a1
pyrus_communis	pycom05g01010 pycom15g03820 pycom15g03830 pycom15g03850 pycom15g04240
rosa_chinensis	RchiOBHm_Chr5g0075231
rosa_laevigata	RLG00000008231 RLG00000008232 RLG00000008235 RLG00000008239 RLG00000008240 RLG00000008241 RLG00000021260 RLG00000021269 RLG00000036539 RLG00000036540 RLG00000037046
rosa_multiflora	Rmu_sc0000955.1_g000047 Rmu_sc0006420.1_g000034
rosa_roxburghii	Rroxscaffold_1G00000870 Rroxscaffold_1G00000880 Rroxscaffold_1G00006320 Rroxscaffold_2G00088990 Rroxscaffold_2G00089000 Rroxscaffold_2G00089010 Rroxscaffold_2G00089100 Rroxscaffold_5G00357190 Rroxscaffold_5G00357200
rosa_rugosa	Rorug04G0118300 Rorug04G0118400 Rorug05G0437400 Rorug05G0439900
rosa_samantha	Rh2AG552900 Rh2AG553000 Rh2BG566800 Rh2CG536900 Rh2DG575800 Rh2DG575900 Rh4AG177300 Rh4AG177400 Rh4AG178100 Rh4AG178300 Rh4AG178400 Rh4AG178500 Rh4BG177100 Rh4BG177200 Rh4BG177700 Rh4CG188300 Rh4CG188700 Rh4CG188800 Rh4CG189000 Rh4CG189100 Rh5AG494400 Rh5AG497000 Rh5AG537400 Rh5BG515800 Rh5BG515900 Rh5BG516000 Rh5CG539600 Rh5CG539700 Rh5CG539800 Rh5DG528600 Rh5DG528700
rosa_wichuraiana	Rw2G045740 Rw4G014830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1239
AccI	GTMKAC	1 cut(s)	1001
AciI	CCGC	3 cut(s)	20, 176, 1112
AclWI	GGATC	8 cut(s)	386, 455, 470, 579, 596, 872, 885, 1020
AcoI	YGGCCR	3 cut(s)	534, 764, 982
AcsI	RAATTY	4 cut(s)	86, 615, 821, 987
AcuI	CTGAAG	1 cut(s)	519
AcvI	CACGTG	1 cut(s)	1037
AfaI	GTAC	3 cut(s)	44, 342, 439
AfiI	CCNNNNNNNGG	3 cut(s)	337, 447, 592
AflIII	ACRYGT	3 cut(s)	242, 1034, 1036
AgsI	TTSAA	4 cut(s)	122, 592, 1052, 1179
AjnI	CCWGG	2 cut(s)	510, 921
AluBI	AGCT	7 cut(s)	454, 628, 859, 898, 919, 1043, 1157
AluI	AGCT	7 cut(s)	454, 628, 859, 898, 919, 1043, 1157
Alw21I	GWGCWC	2 cut(s)	861, 921
Alw26I	GTCTC	2 cut(s)	428, 900
AlwI	GGATC	8 cut(s)	386, 455, 470, 579, 596, 872, 885, 1020
AlwNI	CAGNNNCTG	1 cut(s)	426
Ama87I	CYCGRG	2 cut(s)	1103, 1239
AoxI	GGCC	3 cut(s)	534, 764, 982
ApeKI	GCWGC	1 cut(s)	1109
ApoI	RAATTY	4 cut(s)	86, 615, 821, 987
ArsI	GACNNNNNNTTYG	4 cut(s)	55, 87, 473, 505
AspS9I	GGNCC	3 cut(s)	410, 494, 1087
AsuHPI	GGTGA	4 cut(s)	239, 499, 569, 734
AvaI	CYCGRG	2 cut(s)	1103, 1239
AvaII	GGWCC	3 cut(s)	410, 494, 1087
BaeGI	GKGCMC	1 cut(s)	369
BalI	TGGCCA	2 cut(s)	536, 766
BamHI	GGATCC	1 cut(s)	877
BanII	GRGCYC	2 cut(s)	861, 921
BauI	CACGAG	1 cut(s)	360
BbrPI	CACGTG	1 cut(s)	1037
Bbv12I	GWGCWC	2 cut(s)	861, 921
BbvCI	CCTCAGC	1 cut(s)	1153
BbvI	GCAGC	1 cut(s)	1096
BccI	CCATC	5 cut(s)	46, 971, 1067, 1070, 1179
BceAI	ACGGC	1 cut(s)	54
BciT130I	CCWGG	2 cut(s)	512, 923
BciVI	GTATCC	1 cut(s)	899
BclI	TGATCA	1 cut(s)	1231
BcoDI	GTCTC	2 cut(s)	428, 900
BfaI	CTAG	3 cut(s)	911, 1143, 1223
BfmI	CTRYAG	1 cut(s)	192
BfuI	GTATCC	1 cut(s)	899
BisI	GCNGC	1 cut(s)	1110
BlsI	GCNGC	1 cut(s)	1111
Bme1390I	CCNGG	2 cut(s)	512, 923
Bme18I	GGWCC	3 cut(s)	410, 494, 1087
BmeT110I	CYCGRG	2 cut(s)	1103, 1239
BmgT120I	GGNCC	3 cut(s)	410, 494, 1087
BmiI	GGNNCC	4 cut(s)	189, 205, 411, 879
BmrFI	CCNGG	2 cut(s)	512, 923
BmsI	GCATC	1 cut(s)	438
BpmI	CTGGAG	2 cut(s)	533, 905
Bpu10I	CCTNAGC	1 cut(s)	1153
BpuEI	CTTGAG	1 cut(s)	821
BsaAI	YACGTR	1 cut(s)	1037
BsaBI	GATNNNNATC	3 cut(s)	138, 570, 1080
BsaI	GGTCTC	1 cut(s)	900
BsaJI	CCNNGG	4 cut(s)	446, 464, 510, 922
BsaWI	WCCGGW	2 cut(s)	284, 1084
Bsc4I	CCNNNNNNNGG	3 cut(s)	337, 447, 592
Bse118I	RCCGGY	1 cut(s)	1004
Bse8I	GATNNNNATC	3 cut(s)	138, 570, 1080
BseBI	CCWGG	2 cut(s)	512, 923
BseDI	CCNNGG	4 cut(s)	446, 464, 510, 922
BseGI	GGATG	2 cut(s)	382, 1234
BseJI	GATNNNNATC	3 cut(s)	138, 570, 1080
BseLI	CCNNNNNNNGG	3 cut(s)	337, 447, 592
BseMII	CTCAG	3 cut(s)	128, 396, 1167
BseRI	GAGGAG	1 cut(s)	1211
BseSI	GKGCMC	1 cut(s)	369
BseXI	GCAGC	1 cut(s)	1096
Bsh1285I	CGRYCG	3 cut(s)	61, 436, 1072
BshFI	GGCC	3 cut(s)	536, 766, 984
BsiEI	CGRYCG	3 cut(s)	61, 436, 1072
BsiHKAI	GWGCWC	2 cut(s)	861, 921
BsiHKCI	CYCGRG	2 cut(s)	1103, 1239
BsiSI	CCGG	3 cut(s)	285, 1005, 1085
BslFI	GGGAC	1 cut(s)	1228
BslI	CCNNNNNNNGG	3 cut(s)	337, 447, 592
BsmAI	GTCTC	2 cut(s)	428, 900
BsmFI	GGGAC	1 cut(s)	1228
BsmI	GAATGC	1 cut(s)	403
BsnI	GGCC	3 cut(s)	536, 766, 984
Bso31I	GGTCTC	1 cut(s)	900
BsoBI	CYCGRG	2 cut(s)	1103, 1239
Bsp1286I	GDGCHC	3 cut(s)	369, 861, 921
BspACI	CCGC	3 cut(s)	20, 176, 1112
BspANI	GGCC	3 cut(s)	536, 766, 984
BspCNI	CTCAG	3 cut(s)	127, 397, 1166
BspLI	GGNNCC	4 cut(s)	189, 205, 411, 879
BspPI	GGATC	8 cut(s)	386, 455, 470, 579, 596, 872, 885, 1020
BspTNI	GGTCTC	1 cut(s)	900
BsrFI	RCCGGY	1 cut(s)	1004
BssAI	RCCGGY	1 cut(s)	1004
BssECI	CCNNGG	4 cut(s)	446, 464, 510, 922
BssNAI	GTATAC	1 cut(s)	1002
BssSI	CACGAG	1 cut(s)	360
BssT1I	CCWWGG	1 cut(s)	446
Bst1107I	GTATAC	1 cut(s)	1002
Bst2BI	CACGAG	1 cut(s)	360
Bst2UI	CCWGG	2 cut(s)	512, 923
Bst4CI	ACNGT	7 cut(s)	323, 332, 437, 647, 959, 1136, 1187
BstBAI	YACGTR	1 cut(s)	1037
BstC8I	GCNNGC	1 cut(s)	12
BstDEI	CTNAG	3 cut(s)	114, 405, 1153
BstENI	CCTNNNNNAGG	1 cut(s)	590
BstF5I	GGATG	2 cut(s)	382, 1234
BstMAI	GTCTC	2 cut(s)	428, 900
BstMCI	CGRYCG	3 cut(s)	61, 436, 1072
BstNI	CCWGG	2 cut(s)	512, 923
BstSCI	CCNGG	2 cut(s)	510, 921
BstSFI	CTRYAG	1 cut(s)	192
BstSLI	GKGCMC	1 cut(s)	369
BstV1I	GCAGC	1 cut(s)	1096
BstX2I	RGATCY	2 cut(s)	877, 1012
BstYI	RGATCY	2 cut(s)	877, 1012
BstZ17I	GTATAC	1 cut(s)	1002
BsuI	GTATCC	1 cut(s)	899
BsuRI	GGCC	3 cut(s)	536, 766, 984
BtgZI	GCGATG	1 cut(s)	570
BtsCI	GGATG	2 cut(s)	382, 1234
BtsI	GCAGTG	2 cut(s)	298, 861
BtsIMutI	CAGTG	4 cut(s)	298, 643, 861, 1095
Cac8I	GCNNGC	1 cut(s)	12
CaiI	CAGNNNCTG	1 cut(s)	426
Cfr10I	RCCGGY	1 cut(s)	1004
Cfr13I	GGNCC	3 cut(s)	410, 494, 1087
Csp6I	GTAC	3 cut(s)	43, 341, 438
CspCI	CAANNNNNGTGG	2 cut(s)	734, 769
CviAII	CATG	5 cut(s)	131, 350, 854, 1165, 1229
CviQI	GTAC	3 cut(s)	43, 341, 438
DdeI	CTNAG	3 cut(s)	114, 405, 1153
EaeI	YGGCCR	3 cut(s)	534, 764, 982
Ecl136II	GAGCTC	2 cut(s)	859, 919
Eco130I	CCWWGG	1 cut(s)	446
Eco24I	GRGCYC	2 cut(s)	861, 921
Eco31I	GGTCTC	1 cut(s)	900
Eco47I	GGWCC	3 cut(s)	410, 494, 1087
Eco53kI	GAGCTC	2 cut(s)	859, 919
Eco57I	CTGAAG	1 cut(s)	519
Eco72I	CACGTG	1 cut(s)	1037
Eco88I	CYCGRG	2 cut(s)	1103, 1239
EcoICRI	GAGCTC	2 cut(s)	859, 919
EcoNI	CCTNNNNNAGG	1 cut(s)	590
EcoRI	GAATTC	1 cut(s)	86
EcoRII	CCWGG	2 cut(s)	510, 921
EcoT14I	CCWWGG	1 cut(s)	446
EcoT38I	GRGCYC	2 cut(s)	861, 921
ErhI	CCWWGG	1 cut(s)	446
FaeI	CATG	5 cut(s)	134, 353, 857, 1168, 1232
FaqI	GGGAC	1 cut(s)	1228
FatI	CATG	5 cut(s)	130, 349, 853, 1164, 1228
FbaI	TGATCA	1 cut(s)	1231
FblI	GTMKAC	1 cut(s)	1001
Fnu4HI	GCNGC	1 cut(s)	1110
FokI	GGATG	2 cut(s)	389, 1221
FriOI	GRGCYC	2 cut(s)	861, 921
Fsp4HI	GCNGC	1 cut(s)	1110
FspBI	CTAG	3 cut(s)	911, 1143, 1223
GluI	GCNGC	1 cut(s)	1110
GsuI	CTGGAG	2 cut(s)	533, 905
HaeIII	GGCC	3 cut(s)	536, 766, 984
HapII	CCGG	3 cut(s)	285, 1005, 1085
Hin1II	CATG	5 cut(s)	134, 353, 857, 1168, 1232
HinfI	GANTC	5 cut(s)	230, 263, 269, 281, 356
HpaII	CCGG	3 cut(s)	285, 1005, 1085
HphI	GGTGA	4 cut(s)	239, 499, 569, 734
Hpy166II	GTNNAC	2 cut(s)	742, 1002
Hpy188I	TCNGA	8 cut(s)	268, 274, 310, 396, 406, 465, 882, 1066
Hpy188III	TCNNGA	4 cut(s)	161, 497, 905, 1073
Hpy8I	GTNNAC	2 cut(s)	742, 1002
Hpy99I	CGWCG	3 cut(s)	348, 953, 1070
HpyAV	CCTTC	4 cut(s)	22, 253, 617, 779
HpyCH4III	ACNGT	7 cut(s)	323, 332, 437, 647, 959, 1136, 1187
HpyCH4IV	ACGT	4 cut(s)	242, 343, 738, 1036
HpyCH4V	TGCA	5 cut(s)	10, 93, 130, 429, 708
HpyF3I	CTNAG	3 cut(s)	114, 405, 1153
HpySE526I	ACGT	4 cut(s)	242, 343, 738, 1036
Hsp92II	CATG	5 cut(s)	134, 353, 857, 1168, 1232
Ksp22I	TGATCA	1 cut(s)	1231
LmnI	GCTCC	1 cut(s)	924
Lsp1109I	GCAGC	1 cut(s)	1096
LweI	GCATC	1 cut(s)	438
MaeI	CTAG	3 cut(s)	911, 1143, 1223
MaeII	ACGT	4 cut(s)	242, 343, 738, 1036
MaeIII	GTNAC	6 cut(s)	245, 647, 674, 689, 700, 953
MboII	GAAGA	3 cut(s)	149, 309, 575
MflI	RGATCY	2 cut(s)	877, 1012
MhlI	GDGCHC	3 cut(s)	369, 861, 921
MlsI	TGGCCA	2 cut(s)	536, 766
MluNI	TGGCCA	2 cut(s)	536, 766
MmeI	TCCRAC	2 cut(s)	855, 1089
Mox20I	TGGCCA	2 cut(s)	536, 766
MscI	TGGCCA	2 cut(s)	536, 766
MseI	TTAA	2 cut(s)	807, 836
MslI	CAYNNNNRTG	1 cut(s)	1035
Msp20I	TGGCCA	2 cut(s)	536, 766
MspI	CCGG	3 cut(s)	285, 1005, 1085
MspR9I	CCNGG	2 cut(s)	512, 923
Mva1269I	GAATGC	1 cut(s)	403
MvaI	CCWGG	2 cut(s)	512, 923
NlaIII	CATG	5 cut(s)	134, 353, 857, 1168, 1232
NlaIV	GGNNCC	4 cut(s)	189, 205, 411, 879
NmuCI	GTSAC	2 cut(s)	647, 689
PaeR7I	CTCGAG	2 cut(s)	1103, 1239
PcsI	WCGNNNNNNNCGW	1 cut(s)	67
PctI	GAATGC	1 cut(s)	403
PfeI	GAWTC	5 cut(s)	230, 263, 269, 281, 356
PflFI	GACNNNGTC	1 cut(s)	487
PkrI	GCNGC	1 cut(s)	1111
Ple19I	CGATCG	2 cut(s)	61, 1072
PmaCI	CACGTG	1 cut(s)	1037
PmlI	CACGTG	1 cut(s)	1037
Ppu21I	YACGTR	1 cut(s)	1037
Psp124BI	GAGCTC	2 cut(s)	861, 921
Psp6I	CCWGG	2 cut(s)	510, 921
PspCI	CACGTG	1 cut(s)	1037
PspGI	CCWGG	2 cut(s)	510, 921
PspN4I	GGNNCC	4 cut(s)	189, 205, 411, 879
PspPI	GGNCC	3 cut(s)	410, 494, 1087
PspXI	VCTCGAGB	2 cut(s)	1103, 1239
PstNI	CAGNNNCTG	1 cut(s)	426
PsuI	RGATCY	2 cut(s)	877, 1012
PsyI	GACNNNGTC	1 cut(s)	487
PvuI	CGATCG	2 cut(s)	61, 1072
RsaI	GTAC	3 cut(s)	44, 342, 439
RsaNI	GTAC	3 cut(s)	43, 341, 438
RseI	CAYNNNNRTG	1 cut(s)	1035
SacI	GAGCTC	2 cut(s)	861, 921
SaqAI	TTAA	2 cut(s)	807, 836
SatI	GCNGC	1 cut(s)	1110
Sau96I	GGNCC	3 cut(s)	410, 494, 1087
ScrFI	CCNGG	2 cut(s)	512, 923
SduI	GDGCHC	3 cut(s)	369, 861, 921
SfaNI	GCATC	1 cut(s)	438
SfcI	CTRYAG	1 cut(s)	192
Sfr274I	CTCGAG	2 cut(s)	1103, 1239
SinI	GGWCC	3 cut(s)	410, 494, 1087
SlaI	CTCGAG	2 cut(s)	1103, 1239
SmiMI	CAYNNNNRTG	1 cut(s)	1035
SmlI	CTYRAG	3 cut(s)	800, 1103, 1239
SmoI	CTYRAG	3 cut(s)	800, 1103, 1239
SsiI	CCGC	3 cut(s)	20, 176, 1112
SspI	AATATT	3 cut(s)	79, 172, 1025
SspMI	CTAG	3 cut(s)	911, 1143, 1223
SstI	GAGCTC	2 cut(s)	861, 921
StyD4I	CCNGG	2 cut(s)	510, 921
StyI	CCWWGG	1 cut(s)	446
TaaI	ACNGT	7 cut(s)	323, 332, 437, 647, 959, 1136, 1187
TaiI	ACGT	4 cut(s)	245, 346, 741, 1039
TaqI	TCGA	5 cut(s)	61, 432, 904, 1104, 1240
TatI	WGTACW	1 cut(s)	42
TfiI	GAWTC	5 cut(s)	230, 263, 269, 281, 356
Tru1I	TTAA	2 cut(s)	807, 836
Tru9I	TTAA	2 cut(s)	807, 836
TscAI	CASTG	4 cut(s)	298, 650, 868, 1102
TseFI	GTSAC	2 cut(s)	647, 689
TseI	GCWGC	1 cut(s)	1109
Tsp45I	GTSAC	2 cut(s)	647, 689
TspDTI	ATGAA	2 cut(s)	99, 272
TspRI	CASTG	4 cut(s)	298, 650, 868, 1102
Tth111I	GACNNNGTC	1 cut(s)	487
VpaK11BI	GGWCC	3 cut(s)	410, 494, 1087
XagI	CCTNNNNNAGG	1 cut(s)	590
XapI	RAATTY	4 cut(s)	86, 615, 821, 987
XcmI	CCANNNNNNNNNTGG	1 cut(s)	30
XhoI	CTCGAG	2 cut(s)	1103, 1239
XmiI	GTMKAC	1 cut(s)	1001
XspI	CTAG	3 cut(s)	911, 1143, 1223

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.