Rroxscaffold_2G00089000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000563
Physical Location & Seq
Forward (+)
10889432 .. 10892250
2819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089000.1

Sequence Viewer

Length: 447 bp
ATGGACGACAGAAAATTAAGAGAGATTTTGCTCTCGGTTTTGATTTCTACTATTCGCGATACCATGGCCGGTGAACCAATCCTGCAAAGACCTTCCTCATCCCCTGAGTCTGTGGCCAGTTCTTTTGCGGCTACAAGCTCTTCATCAGCATTACCAAATTTGTTACTGCTAGTTTTGGTAGGCCGGGTGGTTCATACATTTTTGAATTTTGACACTAGTGAAGAGGAAGGGGAGGATGCGAAGAATATACACGATGGTGTTATAAAGAGGTTCAAGACTTTCACAGAATTTGAGGGATTATTAATTAGCGTGACGGCTTATGGTTATAACGTAACTAAGGTAGTTCTATATAAGTTGGATTCGAATGGAATCCCCAACCGCGAGTCGTATCAGGTGCTTCATGATATCACTGCAGAGATTTATCTTCCTGACCTAATAATATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.39

Weight (kDa)

4.87

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 263, 327
AccII CGCG 2 cut(s) 57, 381
AciI CCGC 2 cut(s) 128, 379
AcoI YGGCCR 2 cut(s) 66, 114
AcsI RAATTY 3 cut(s) 157, 205, 287
AgsI TTSAA 2 cut(s) 205, 274
AhlI ACTAGT 1 cut(s) 215
AleI CACNNNNGTG 1 cut(s) 255
AluBI AGCT 1 cut(s) 138
AluI AGCT 1 cut(s) 138
AoxI GGCC 3 cut(s) 66, 114, 181
ApoI RAATTY 3 cut(s) 157, 205, 287
AseI ATTAAT 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 83
AsuII TTCGAA 1 cut(s) 362
BalI TGGCCA 1 cut(s) 116
BccI CCATC 1 cut(s) 248
BceAI ACGGC 1 cut(s) 330
BcnI CCSGG 1 cut(s) 185
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 2 cut(s) 170, 216
BfmI CTRYAG 1 cut(s) 411
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 226
Bpu14I TTCGAA 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 63
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 63
BseGI GGATG 2 cut(s) 98, 241
BseMII CTCAG 1 cut(s) 96
BseNI ACTGG 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 57, 381
BshFI GGCC 3 cut(s) 68, 116, 183
BsiSI CCGG 2 cut(s) 69, 184
BsnI GGCC 3 cut(s) 68, 116, 183
Bsp119I TTCGAA 1 cut(s) 362
Bsp19I CCATGG 1 cut(s) 63
Bsp68I TCGCGA 1 cut(s) 57
BspACI CCGC 2 cut(s) 128, 379
BspANI GGCC 3 cut(s) 68, 116, 183
BspCNI CTCAG 1 cut(s) 97
BspFNI CGCG 2 cut(s) 57, 381
BspHI TCATGA 1 cut(s) 400
BspMAI CTGCAG 1 cut(s) 415
BspQI GCTCTTC 1 cut(s) 145
BspT104I TTCGAA 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 1 cut(s) 117
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 63
Bst6I CTCTTC 2 cut(s) 145, 216
BstBI TTCGAA 1 cut(s) 362
BstDEI CTNAG 2 cut(s) 105, 336
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 98, 241
BstFNI CGCG 2 cut(s) 57, 381
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 411
BstUI CGCG 2 cut(s) 57, 381
BsuRI GGCC 3 cut(s) 68, 116, 183
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 2 cut(s) 98, 241
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 408
BtuMI TCGCGA 1 cut(s) 57
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 68
CviAII CATG 2 cut(s) 64, 401
CviJI RGCY 6 cut(s) 68, 116, 131, 138, 183, 317
CviKI_1 RGCY 6 cut(s) 68, 116, 131, 138, 183, 317
DdeI CTNAG 2 cut(s) 105, 336
EaeI YGGCCR 2 cut(s) 66, 114
Eam1104I CTCTTC 2 cut(s) 145, 216
EarI CTCTTC 2 cut(s) 145, 216
Eco130I CCWWGG 1 cut(s) 63
Eco32I GATATC 1 cut(s) 406
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 2 cut(s) 67, 404
FaiI YATR 9 cut(s) 65, 195, 248, 263, 321, 327, 349, 351, 402
FatI CATG 2 cut(s) 63, 400
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 2 cut(s) 85, 248
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 2 cut(s) 170, 216
GluI GCNGC 1 cut(s) 129
HaeIII GGCC 3 cut(s) 68, 116, 183
HapII CCGG 2 cut(s) 69, 184
Hin1II CATG 2 cut(s) 67, 404
HinfI GANTC 4 cut(s) 107, 359, 369, 383
HpaII CCGG 2 cut(s) 69, 184
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 1 cut(s) 74
Hpy188III TCNNGA 4 cut(s) 56, 274, 401, 428
Hpy8I GTNNAC 1 cut(s) 74
HpyAV CCTTC 2 cut(s) 102, 221
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 2 cut(s) 85, 413
HpyF3I CTNAG 2 cut(s) 105, 336
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 67, 404
LguI GCTCTTC 1 cut(s) 145
LpnPI CCDG 7 cut(s) 82, 95, 117, 130, 197, 377, 441
LweI GCATC 1 cut(s) 226
MaeI CTAG 2 cut(s) 170, 216
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 3 cut(s) 162, 310, 331
MboII GAAGA 4 cut(s) 132, 233, 253, 416
MlsI TGGCCA 1 cut(s) 116
MluCI AATT 5 cut(s) 14, 157, 205, 287, 303
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 2 cut(s) 116, 392
MmeI TCCRAC 1 cut(s) 336
MnlI CCTC 5 cut(s) 106, 217, 226, 261, 286
Mox20I TGGCCA 1 cut(s) 116
MscI TGGCCA 1 cut(s) 116
MseI TTAA 3 cut(s) 17, 302, 445
MslI CAYNNNNRTG 1 cut(s) 255
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 2 cut(s) 69, 184
MspR9I CCNGG 1 cut(s) 185
MvnI CGCG 2 cut(s) 57, 381
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 63
NlaIII CATG 2 cut(s) 67, 404
NmuCI GTSAC 1 cut(s) 310
NruI TCGCGA 1 cut(s) 57
NspV TTCGAA 1 cut(s) 362
OliI CACNNNNGTG 1 cut(s) 255
PagI TCATGA 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 145
PfeI GAWTC 2 cut(s) 359, 369
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 2 cut(s) 115, 391
PpsI GAGTC 2 cut(s) 115, 391
PshBI ATTAAT 1 cut(s) 302
PsiI TTATAA 2 cut(s) 263, 327
PstI CTGCAG 1 cut(s) 415
RruI TCGCGA 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 3 cut(s) 17, 302, 445
SatI GCNGC 1 cut(s) 129
SchI GAGTC 2 cut(s) 116, 392
ScrFI CCNGG 1 cut(s) 185
SetI ASST 7 cut(s) 94, 140, 272, 333, 342, 396, 435
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 1 cut(s) 411
SfuI TTCGAA 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 255
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 5 cut(s) 14, 157, 205, 287, 303
SsiI CCGC 2 cut(s) 128, 379
SspMI CTAG 2 cut(s) 170, 216
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 63
TaiI ACGT 1 cut(s) 333
TaqI TCGA 1 cut(s) 362
TasI AATT 5 cut(s) 14, 157, 205, 287, 303
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 2 cut(s) 359, 369
Tru1I TTAA 3 cut(s) 17, 302, 445
Tru9I TTAA 3 cut(s) 17, 302, 445
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 3 cut(s) 132, 182, 389
TspRI CASTG 1 cut(s) 415
VspI ATTAAT 1 cut(s) 302
XapI RAATTY 3 cut(s) 157, 205, 287
XspI CTAG 2 cut(s) 170, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.