Rh4AG177400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000563
Physical Location & Seq
Forward (+)
44568242 .. 44568670
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG177400.1

Sequence Viewer

Length: 429 bp
ATGTTTTCATGGCATCCTTTGTTAAGTAACCCTCAAGTTTTCTTGGGTAAATGTGATGATAACCTTATGTGGTTGGTGTATTGTGGCAAGGATAATGTCCAGGGATTTTCTTGTATAGGCCTCGCAGTGTTCCGAGTTTCTATGTCATATGACCATGCTGGCAAAGTAGTGTTTTTGGTAGATATTGAGGCTGCAAATTTTATCGATTCAAATAGTTATGAGGTTCAAGGCGCCAAGGAGACCACTCTAGTTTACTCAGATTCTGCCATGTGCCATGATAATTTGAAGACCAAGGATTGGTTTTTGTATTTGGATGTGGGGGATCGACTATGCAGTATAAATATTAGCGAATTCTGCAAAAGGAAGAGCTCTTTCATGTCTAAGTTCGGAGTTGTAAATAATGTTAGAAGCTTTTGTTTTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

16.12

Weight (kDa)

5.84

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 297
AclWI GGATC 1 cut(s) 330
AcsI RAATTY 2 cut(s) 196, 350
AcyI GRCGYC 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 297
AgsI TTSAA 3 cut(s) 210, 227, 286
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 2 cut(s) 369, 411
AluI AGCT 2 cut(s) 369, 411
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 1 cut(s) 330
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 2 cut(s) 196, 350
AspLEI GCGC 1 cut(s) 233
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 371
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 178
BciT130I CCWGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 1 cut(s) 248
BfoI RGCGCY 1 cut(s) 234
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 18
Bsa29I ATCGAT 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 3 cut(s) 100, 234, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 101
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 3 cut(s) 100, 234, 291
BseGI GGATG 2 cut(s) 13, 319
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 270
BseXI GCAGC 1 cut(s) 178
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 230
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 233
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 1 cut(s) 322
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 269
BspDI ATCGAT 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 232
BspPI GGATC 1 cut(s) 330
BspQI GCTCTTC 1 cut(s) 359
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 233
BssECI CCNNGG 3 cut(s) 100, 234, 291
BssMI GATC 1 cut(s) 322
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 234, 291
Bst2UI CCWGG 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 359
BstACI GRCGYC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 256, 381
BstF5I GGATG 2 cut(s) 13, 319
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 1 cut(s) 325
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 1 cut(s) 322
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 99
BstV1I GCAGC 1 cut(s) 178
BstV2I GAAGAC 1 cut(s) 293
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 1 cut(s) 120
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 2 cut(s) 13, 319
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 204
CviAII CATG 5 cut(s) 9, 155, 268, 275, 376
CviJI RGCY 4 cut(s) 120, 191, 369, 411
CviKI_1 RGCY 4 cut(s) 120, 191, 369, 411
DdeI CTNAG 2 cut(s) 256, 381
DinI GGCGCC 1 cut(s) 232
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Ecl136II GAGCTC 1 cut(s) 369
Eco130I CCWWGG 2 cut(s) 234, 291
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 369
EcoRI GAATTC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 99
EcoT14I CCWWGG 2 cut(s) 234, 291
EcoT38I GRGCYC 1 cut(s) 371
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 234, 291
FaeI CATG 5 cut(s) 12, 158, 271, 278, 379
FatI CATG 5 cut(s) 8, 154, 267, 274, 375
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 232
GluI GCNGC 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 1 cut(s) 120
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 5 cut(s) 12, 158, 271, 278, 379
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 2 cut(s) 206, 260
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 4 cut(s) 134, 259, 389, 428
Hpy8I GTNNAC 1 cut(s) 253
HpyCH4V TGCA 3 cut(s) 194, 333, 357
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 2 cut(s) 256, 381
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 5 cut(s) 12, 158, 271, 278, 379
HspAI GCGC 1 cut(s) 231
KasI GGCGCC 1 cut(s) 230
Kzo9I GATC 1 cut(s) 322
LguI GCTCTTC 1 cut(s) 359
LpnPI CCDG 3 cut(s) 86, 113, 144
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 1 cut(s) 22
MaeI CTAG 1 cut(s) 248
MaeIII GTNAC 1 cut(s) 26
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 2 cut(s) 298, 376
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 3 cut(s) 196, 280, 350
Mly113I GGCGCC 1 cut(s) 231
MnlI CCTC 4 cut(s) 42, 131, 181, 214
MseI TTAA 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 354
NarI GGCGCC 1 cut(s) 231
NdeI CATATG 1 cut(s) 148
NdeII GATC 1 cut(s) 322
NlaIII CATG 5 cut(s) 12, 158, 271, 278, 379
NlaIV GGNNCC 1 cut(s) 232
PceI AGGCCT 1 cut(s) 120
PciSI GCTCTTC 1 cut(s) 359
PfeI GAWTC 2 cut(s) 206, 260
PflMI CCANNNNNTGG 1 cut(s) 297
PkrI GCNGC 1 cut(s) 193
PluTI GGCGCC 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 371
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 1 cut(s) 371
SetI ASST 4 cut(s) 66, 225, 371, 413
SfaNI GCATC 1 cut(s) 22
SfoI GGCGCC 1 cut(s) 232
SmlI CTYRAG 1 cut(s) 33
SmoI CTYRAG 1 cut(s) 33
Sse9I AATT 3 cut(s) 196, 280, 350
SseBI AGGCCT 1 cut(s) 120
SspDI GGCGCC 1 cut(s) 230
SspI AATATT 1 cut(s) 343
SspMI CTAG 1 cut(s) 248
SstI GAGCTC 1 cut(s) 371
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 99
StyI CCWWGG 2 cut(s) 234, 291
TaqI TCGA 2 cut(s) 204, 325
TasI AATT 3 cut(s) 196, 280, 350
TfiI GAWTC 2 cut(s) 206, 260
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 1 cut(s) 132
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 364
TspRI CASTG 1 cut(s) 132
Van91I CCANNNNNTGG 1 cut(s) 297
XapI RAATTY 2 cut(s) 196, 350
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.