MD15G1041200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000563
Physical Location & Seq
Forward (+)
2892629 .. 2895332
2704 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1041200.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGCGGATGGGGCTACTTATCAGGGATTCCAAATTGCATTTTGTTGGGGGTGAGAAGATTGTCAGGCCTGGGATTGTTCCTCACTTGGAGTTAGACCCGAGGTCTTTCAGACGTAGCCCTGAATTTTGCCACCCCAACTCCCATGTCTTCACCTTTGACCCTACATTCCACACCCTCTATAAGGACAGTAACTTTCCTGATCCTTCTGCTCCCAAGTCCTGCCCTATCGTGGTCACCCTTGGGGATAAAAACAAAGTTTATGTCCTTTCAACAGATTCACTCTACCTCTTTACTGGTCATAGTTTAACTGAACCAATTTTCGAGGCTTTTGATTTTGAGTTAAAAACCTGGAAACCTCTCCCTCCCCCCCTTTACTCCCGTCGCGCCCCTATCATGTCCCGCTATGCTATTGGCCGCAAGCTGTATCTTAATACCAGCCATTTTTCATGCATTTTTGATGCAGAAAAAGAAGAATGGGACTATGCCATCCCATTGAAGTTTCCCTATCTTATGTGTTCTGTAGAGTACAATGGCTTTCTAATTGCCATGCCCTTATTCGGAGAAGCAGAAGTTGTTGCCTATAACTTGGACTCATTTGGGCTCACCAATTCTGAAAAGCGTCTAGATCAGCTACAGGAAATATTCAAGGATCCCTTTTACGACCCCAGAGGGTGCTATCTCACCAGTCTTGACGATCATGGCCGCATGTGCCTCCTTTATTCAGGTTTCTCCTCTTCCAACTCTTCCAAAGAGTTTTGGGGACGTGTAGCGGTGTTTCAAGTATCCGTCTCAAACTCTACCGACGGTCCAGTTGTCACTGCAGTTCTAGAGGCTGTCGAAGACTATAACATGCAGGACTTTGTAGTTCGTGATGCCAGTATTTACTCAACATTCATCATGTATGATGATCTGAGCATGGATGATACCGAGGCTGATGTAAGTTTCACGAACTCAACTCTTTCTGGCTTGGTTGCTGTTATGCATGAAGAACCGGAAACTACAGTTTCTGAAACAGGCCAGTTTGTCATGGCATGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

347

Amino Acids

39.35

Weight (kDa)

4.86

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 44 - 162 1.7e-07 FKB95, Kelch-repeats domain
DUF1668 PF07893 47 - 240 2.1e-10 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 384
AciI CCGC 5 cut(s) 4, 400, 415, 703, 770
AclWI GGATC 3 cut(s) 194, 644, 657
AcoI YGGCCR 2 cut(s) 412, 700
AcsI RAATTY 1 cut(s) 122
AfaI GTAC 1 cut(s) 527
AfiI CCNNNNNNNGG 3 cut(s) 181, 229, 557
AflIII ACRYGT 1 cut(s) 763
AgsI TTSAA 4 cut(s) 270, 496, 646, 779
AhdI GACNNNNNGTC 1 cut(s) 100
AjiI CACGTC 1 cut(s) 764
AjnI CCWGG 2 cut(s) 67, 347
AluBI AGCT 2 cut(s) 421, 631
AluI AGCT 2 cut(s) 421, 631
Alw26I GTCTC 1 cut(s) 793
AlwI GGATC 3 cut(s) 194, 644, 657
AlwNI CAGNNNCTG 1 cut(s) 1007
Ama87I CYCGRG 1 cut(s) 97
AoxI GGCC 4 cut(s) 65, 412, 700, 1015
ApoI RAATTY 1 cut(s) 122
ArsI GACNNNNNNTTYG 2 cut(s) 246, 278
AspLEI GCGC 1 cut(s) 386
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 5 cut(s) 62, 142, 226, 595, 673
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 1 cut(s) 806
BamHI GGATCC 1 cut(s) 649
BanII GRGCYC 1 cut(s) 603
BbsI GAAGAC 2 cut(s) 139, 846
BccI CCATC 1 cut(s) 494
BciT130I CCWGG 2 cut(s) 69, 349
BciVI GTATCC 1 cut(s) 793
BcoDI GTCTC 1 cut(s) 793
BfaI CTAG 2 cut(s) 623, 827
BfmI CTRYAG 4 cut(s) 519, 632, 819, 999
BfuI GTATCC 1 cut(s) 793
BisI GCNGC 2 cut(s) 415, 703
BlsI GCNGC 2 cut(s) 416, 704
Bme1390I CCNGG 2 cut(s) 69, 349
Bme18I GGWCC 1 cut(s) 806
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 97
BmgBI CACGTC 1 cut(s) 764
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 1 cut(s) 651
BmrFI CCNGG 2 cut(s) 69, 349
BmsI GCATC 2 cut(s) 448, 862
BpiI GAAGAC 2 cut(s) 139, 846
BsaJI CCNNGG 4 cut(s) 68, 98, 238, 927
BsaWI WCCGGW 1 cut(s) 991
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 229, 557
Bse1I ACTGG 5 cut(s) 298, 684, 809, 876, 1018
BseBI CCWGG 2 cut(s) 69, 349
BseDI CCNNGG 4 cut(s) 68, 98, 238, 927
BseGI GGATG 3 cut(s) 12, 486, 925
BseLI CCNNNNNNNGG 3 cut(s) 181, 229, 557
BseMII CTCAG 1 cut(s) 902
BseNI ACTGG 5 cut(s) 298, 684, 809, 876, 1018
BseRI GAGGAG 1 cut(s) 721
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 4 cut(s) 67, 414, 702, 1017
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 1 cut(s) 992
BslFI GGGAC 3 cut(s) 382, 491, 774
BslI CCNNNNNNNGG 3 cut(s) 181, 229, 557
BsmAI GTCTC 1 cut(s) 793
BsmBI CGTCTC 1 cut(s) 793
BsmFI GGGAC 3 cut(s) 382, 491, 774
BsnI GGCC 4 cut(s) 67, 414, 702, 1017
BsoBI CYCGRG 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 603
Bsp143I GATC 5 cut(s) 199, 625, 649, 694, 907
BspACI CCGC 5 cut(s) 4, 400, 415, 703, 770
BspANI GGCC 4 cut(s) 67, 414, 702, 1017
BspCNI CTCAG 1 cut(s) 903
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 651
BspMAI CTGCAG 1 cut(s) 823
BspPI GGATC 3 cut(s) 194, 644, 657
BsrI ACTGG 5 cut(s) 298, 684, 809, 876, 1018
BssECI CCNNGG 4 cut(s) 68, 98, 238, 927
BssMI GATC 5 cut(s) 199, 625, 649, 694, 907
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 2 cut(s) 69, 349
Bst4CI ACNGT 3 cut(s) 188, 806, 1003
Bst6I CTCTTC 2 cut(s) 739, 748
BstC8I GCNNGC 1 cut(s) 419
BstDEI CTNAG 1 cut(s) 911
BstEII GGTNACC 1 cut(s) 232
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 3 cut(s) 12, 486, 925
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 5 cut(s) 202, 628, 652, 697, 910
BstMAI GTCTC 1 cut(s) 793
BstMBI GATC 5 cut(s) 199, 625, 649, 694, 907
BstMWI GCNNNNNNNGC 2 cut(s) 10, 708
BstNI CCWGG 2 cut(s) 69, 349
BstNSI RCATGY 2 cut(s) 709, 853
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 67, 347
BstSFI CTRYAG 4 cut(s) 519, 632, 819, 999
BstUI CGCG 1 cut(s) 384
BstV2I GAAGAC 2 cut(s) 139, 846
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BsuI GTATCC 1 cut(s) 793
BsuRI GGCC 4 cut(s) 67, 414, 702, 1017
BtrI CACGTC 1 cut(s) 764
BtsCI GGATG 3 cut(s) 12, 486, 925
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 1 cut(s) 816
Cac8I GCNNGC 1 cut(s) 419
CaiI CAGNNNCTG 1 cut(s) 1007
CfoI GCGC 1 cut(s) 386
Cfr13I GGNCC 1 cut(s) 806
CseI GACGC 1 cut(s) 608
Csp6I GTAC 1 cut(s) 526
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 1 cut(s) 911
DpnI GATC 5 cut(s) 201, 627, 651, 696, 909
DpnII GATC 5 cut(s) 199, 625, 649, 694, 907
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 2 cut(s) 412, 700
Eam1104I CTCTTC 2 cut(s) 739, 748
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 2 cut(s) 739, 748
Eco130I CCWWGG 1 cut(s) 238
Eco147I AGGCCT 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 603
Eco47I GGWCC 1 cut(s) 806
Eco88I CYCGRG 1 cut(s) 97
Eco91I GGTNACC 1 cut(s) 232
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 67, 347
EcoT14I CCWWGG 1 cut(s) 238
EcoT22I ATGCAT 2 cut(s) 452, 984
EcoT38I GRGCYC 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 238
Esp3I CGTCTC 1 cut(s) 793
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 3 cut(s) 382, 491, 774
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 2 cut(s) 415, 703
FokI GGATG 3 cut(s) 19, 473, 932
FriOI GRGCYC 1 cut(s) 603
Fsp4HI GCNGC 2 cut(s) 415, 703
FspBI CTAG 2 cut(s) 623, 827
GlaI GCGC 1 cut(s) 385
GluI GCNGC 2 cut(s) 415, 703
HaeIII GGCC 4 cut(s) 67, 414, 702, 1017
HapII CCGG 1 cut(s) 992
HgaI GACGC 1 cut(s) 608
HhaI GCGC 1 cut(s) 386
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HinfI GANTC 3 cut(s) 26, 275, 590
HpaII CCGG 1 cut(s) 992
HphI GGTGA 5 cut(s) 62, 142, 226, 595, 673
Hpy188I TCNGA 5 cut(s) 110, 560, 613, 912, 1009
Hpy188III TCNNGA 6 cut(s) 197, 623, 689, 827, 869, 946
Hpy99I CGWCG 2 cut(s) 384, 806
HpyAV CCTTC 1 cut(s) 213
HpyCH4III ACNGT 3 cut(s) 188, 806, 1003
HpyCH4IV ACGT 2 cut(s) 112, 763
HpyCH4V TGCA 6 cut(s) 37, 450, 461, 821, 853, 982
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 708
HpyF3I CTNAG 1 cut(s) 911
HpySE526I ACGT 2 cut(s) 112, 763
HspAI GCGC 1 cut(s) 384
Kzo9I GATC 5 cut(s) 199, 625, 649, 694, 907
LmnI GCTCC 1 cut(s) 214
LweI GCATC 2 cut(s) 448, 862
MaeI CTAG 2 cut(s) 623, 827
MaeII ACGT 2 cut(s) 112, 763
MaeIII GTNAC 3 cut(s) 188, 232, 814
MalI GATC 5 cut(s) 201, 627, 651, 696, 909
MboI GATC 5 cut(s) 199, 625, 649, 694, 907
MboII GAAGA 7 cut(s) 67, 139, 482, 726, 735, 851, 998
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 603
MluCI AATT 5 cut(s) 32, 122, 315, 540, 607
MlyI GAGTC 1 cut(s) 584
MmeI TCCRAC 1 cut(s) 762
Mph1103I ATGCAT 2 cut(s) 452, 984
MseI TTAA 3 cut(s) 305, 341, 429
MspI CCGG 1 cut(s) 992
MspR9I CCNGG 2 cut(s) 69, 349
MvaI CCWGG 2 cut(s) 69, 349
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 10, 708
NdeII GATC 5 cut(s) 199, 625, 649, 694, 907
NlaIV GGNNCC 1 cut(s) 651
NmuCI GTSAC 2 cut(s) 232, 814
NsiI ATGCAT 2 cut(s) 452, 984
NspI RCATGY 2 cut(s) 709, 853
PceI AGGCCT 1 cut(s) 67
PfeI GAWTC 2 cut(s) 26, 275
PkrI GCNGC 2 cut(s) 416, 704
PleI GAGTC 1 cut(s) 584
PpsI GAGTC 1 cut(s) 584
Psp6I CCWGG 2 cut(s) 67, 347
PspEI GGTNACC 1 cut(s) 232
PspGI CCWGG 2 cut(s) 67, 347
PspN4I GGNNCC 1 cut(s) 651
PspPI GGNCC 1 cut(s) 806
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 1007
PsuI RGATCY 1 cut(s) 649
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SaqAI TTAA 3 cut(s) 305, 341, 429
SatI GCNGC 2 cut(s) 415, 703
Sau3AI GATC 5 cut(s) 199, 625, 649, 694, 907
Sau96I GGNCC 1 cut(s) 806
SchI GAGTC 1 cut(s) 584
ScrFI CCNGG 2 cut(s) 69, 349
SduI GDGCHC 1 cut(s) 603
SfaNI GCATC 2 cut(s) 448, 862
SfcI CTRYAG 4 cut(s) 519, 632, 819, 999
SinI GGWCC 1 cut(s) 806
Sse9I AATT 5 cut(s) 32, 122, 315, 540, 607
SseBI AGGCCT 1 cut(s) 67
SsiI CCGC 5 cut(s) 4, 400, 415, 703, 770
SspI AATATT 1 cut(s) 642
SspMI CTAG 2 cut(s) 623, 827
StuI AGGCCT 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 67, 347
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 3 cut(s) 188, 806, 1003
TaiI ACGT 2 cut(s) 115, 766
TaqI TCGA 2 cut(s) 321, 837
TasI AATT 5 cut(s) 32, 122, 315, 540, 607
TatI WGTACW 1 cut(s) 525
TauI GCSGC 2 cut(s) 417, 705
TfiI GAWTC 2 cut(s) 26, 275
Tru1I TTAA 3 cut(s) 305, 341, 429
Tru9I TTAA 3 cut(s) 305, 341, 429
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 2 cut(s) 232, 814
Tsp45I GTSAC 2 cut(s) 232, 814
TspDTI ATGAA 3 cut(s) 435, 883, 999
TspGWI ACGGA 1 cut(s) 775
TspRI CASTG 1 cut(s) 823
VpaK11BI GGWCC 1 cut(s) 806
XagI CCTNNNNNAGG 1 cut(s) 179
XapI RAATTY 1 cut(s) 122
XbaI TCTAGA 2 cut(s) 622, 826
XceI RCATGY 2 cut(s) 709, 853
XspI CTAG 2 cut(s) 623, 827
Zsp2I ATGCAT 2 cut(s) 452, 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.