RLG00000008232

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000563
Physical Location & Seq
Reverse (-)
27483261 .. 27484710
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008232

Sequence Viewer

Length: 1032 bp
ATGTCGATGAAGAAGGATATGTTCTTGTACTTGAATGTGAACGACCGACTTTTGGGTCTAAATCTTAGAGAGTTCAGCGAAAGGTACTCTTCCAAGCCTGAGTTTGAAGTTGTTCAGGTTTTGAATGATCCCAACCTCCGCCGTCCAGGTACAAATTCCGATATCGCAAAGATAGATTGTATGACTCTGTGTAATTCCAACTTGTATATGATGAGGGTAACTGAAAATCACCGTGGGTCTTGTAATTTTGAGTTTGATGTTTATTCCTTTGATCCCACTGGGGCAGATGACCGTAAATCCATCCGCCTATGTGACAAAACTGCCTCACCTAGATTGATGTGTGGTAATCGTCCTATAGTGTTCACCCTCGATGAGAAAATCTATGTTCTGGGAGTACCATCATATTCATACAGCAGACGTGGTGATGATGTGGAGGAAGAGCCACAACTTTTTTGGGTTTTTGATCCTTTTCACGAATCCTGGAAGCCACTCCCTCATCCTCTCTTCTACAATCATGATACGTACTTTGTGTATTATGATGTTTCTTATGAATTTGGTGAAGGCTTTTTAATTGCTGTGCCGGAGAGGAAGAGCAATGAACAGATGAGGCACGAGCTTGTTGCTTATAAGTTGGATGTGAACGGGATCCCTCAGCCGCATCAGGTGCTACACGAGCTTACGGGGATGTTTTCATGGCGTCCTTTGTTAATTAACCCTCAAGTTCTTTTGGGTAAATGTGATGATAACCTTATGTGGTTGGTGTGTTTTGGCAAGGATAATGTCCAGAAATTTTCTTGTATAGGTGTCGCAGTGTTTCGAGTTTCTATGTCATATGACAATGCTGGCAAAGTAGTGTTTTTGGTAGATATTGAGGCTGCGGATTTTATCTATTCAAATAGTTACGAGGTTCAAGGCGCCAAGGAGACCACTCTAGTTTGCTCAGCTTCTGCCATGTGCCATGATAATTTGAAGACCAAGGATTGGTTTTTGTATTTGAATATGGGGCCATGGGGGATCGACTATGCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

39.69

Weight (kDa)

5.17

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
AasI GACNNNNNNGTC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 914
AccB7I CCANNNNNTGG 1 cut(s) 981
AciI CCGC 4 cut(s) 139, 304, 656, 878
AclWI GGATC 6 cut(s) 122, 266, 458, 640, 653, 1022
AcsI RAATTY 3 cut(s) 154, 551, 788
AcyI GRCGYC 2 cut(s) 697, 915
AfaI GTAC 5 cut(s) 29, 86, 151, 396, 524
AfiI CCNNNNNNNGG 2 cut(s) 52, 981
AgsI TTSAA 7 cut(s) 34, 107, 124, 894, 911, 970, 997
AjiI CACGTC 1 cut(s) 419
AjnI CCWGG 2 cut(s) 145, 479
AluBI AGCT 3 cut(s) 616, 676, 944
AluI AGCT 3 cut(s) 616, 676, 944
Alw26I GTCTC 1 cut(s) 917
AlwI GGATC 6 cut(s) 122, 266, 458, 640, 653, 1022
AlwNI CAGNNNCTG 1 cut(s) 947
AoxI GGCC 1 cut(s) 1004
ApeKI GCWGC 1 cut(s) 875
ApoI RAATTY 3 cut(s) 154, 551, 788
Asp700I GAANNNNTTC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 917
AspS9I GGNCC 1 cut(s) 1004
AsuHPI GGTGA 5 cut(s) 221, 318, 355, 434, 569
BamHI GGATCC 1 cut(s) 645
BanI GGYRCC 1 cut(s) 914
BauI CACGAG 2 cut(s) 611, 671
BbsI GAAGAC 1 cut(s) 977
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 862
BccI CCATC 2 cut(s) 308, 406
BceAI ACGGC 1 cut(s) 126
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 2 cut(s) 147, 481
BcoDI GTCTC 1 cut(s) 917
BfaI CTAG 2 cut(s) 330, 932
BfmI CTRYAG 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 918
BisI GCNGC 2 cut(s) 656, 876
BlpI GCTNAGC 1 cut(s) 940
BlsI GCNGC 2 cut(s) 657, 877
Bme1390I CCNGG 2 cut(s) 147, 481
BmgBI CACGTC 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 1004
BmiI GGNNCC 3 cut(s) 647, 916, 1005
BmrFI CCNGG 2 cut(s) 147, 481
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 977
Bpu10I CCTNAGC 1 cut(s) 651
Bpu1102I GCTNAGC 1 cut(s) 940
BpuEI CTTGAG 1 cut(s) 702
BsaAI YACGTR 1 cut(s) 522
BsaHI GRCGYC 2 cut(s) 697, 915
BsaI GGTCTC 1 cut(s) 917
BsaJI CCNNGG 4 cut(s) 232, 918, 975, 1007
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 981
Bse1I ACTGG 1 cut(s) 283
Bse3DI GCAATG 1 cut(s) 601
BseBI CCWGG 2 cut(s) 147, 481
BseDI CCNNGG 4 cut(s) 232, 918, 975, 1007
BseGI GGATG 4 cut(s) 300, 496, 640, 690
BseLI CCNNNNNNNGG 2 cut(s) 52, 981
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 3 cut(s) 90, 665, 954
BseNI ACTGG 1 cut(s) 283
BseXI GCAGC 1 cut(s) 862
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 1006
BshNI GGYRCC 1 cut(s) 914
BsiEI CGRYCG 1 cut(s) 46
BsiSI CCGG 1 cut(s) 581
BslI CCNNNNNNNGG 2 cut(s) 52, 981
BsmAI GTCTC 1 cut(s) 917
BsnI GGCC 1 cut(s) 1006
Bso31I GGTCTC 1 cut(s) 917
Bsp143I GATC 5 cut(s) 127, 271, 463, 645, 1014
Bsp1720I GCTNAGC 1 cut(s) 940
Bsp19I CCATGG 1 cut(s) 1007
BspACI CCGC 4 cut(s) 139, 304, 656, 878
BspANI GGCC 1 cut(s) 1006
BspCNI CTCAG 3 cut(s) 91, 664, 953
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 3 cut(s) 647, 916, 1005
BspPI GGATC 6 cut(s) 122, 266, 458, 640, 653, 1022
BspQI GCTCTTC 2 cut(s) 432, 584
BspT107I GGYRCC 1 cut(s) 914
BspTNI GGTCTC 1 cut(s) 917
BsrDI GCAATG 1 cut(s) 601
BsrI ACTGG 1 cut(s) 283
BssECI CCNNGG 4 cut(s) 232, 918, 975, 1007
BssMI GATC 5 cut(s) 127, 271, 463, 645, 1014
BssNI GRCGYC 2 cut(s) 697, 915
BssSI CACGAG 2 cut(s) 611, 671
BssT1I CCWWGG 3 cut(s) 918, 975, 1007
Bst2BI CACGAG 2 cut(s) 611, 671
Bst2UI CCWGG 2 cut(s) 147, 481
Bst4CI ACNGT 2 cut(s) 233, 293
Bst6I CTCTTC 4 cut(s) 94, 432, 509, 584
BstACI GRCGYC 2 cut(s) 697, 915
BstAPI GCANNNNNTGC 1 cut(s) 664
BstBAI YACGTR 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 844
BstDEI CTNAG 4 cut(s) 65, 99, 651, 940
BstDSI CCRYGG 2 cut(s) 232, 1007
BstF5I GGATG 4 cut(s) 300, 496, 640, 690
BstH2I RGCGCY 1 cut(s) 918
BstHHI GCGC 1 cut(s) 917
BstKTI GATC 5 cut(s) 130, 274, 466, 648, 1017
BstMAI GTCTC 1 cut(s) 917
BstMBI GATC 5 cut(s) 127, 271, 463, 645, 1014
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 2 cut(s) 664, 673
BstNI CCWGG 2 cut(s) 147, 481
BstSCI CCNGG 2 cut(s) 145, 479
BstSFI CTRYAG 1 cut(s) 354
BstSNI TACGTA 1 cut(s) 522
BstV1I GCAGC 1 cut(s) 862
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 1 cut(s) 645
BstYI RGATCY 1 cut(s) 645
BsuRI GGCC 1 cut(s) 1006
BtgI CCRYGG 2 cut(s) 232, 1007
BtrI CACGTC 1 cut(s) 419
BtsCI GGATG 4 cut(s) 300, 496, 640, 690
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 2 cut(s) 276, 816
Cac8I GCNNGC 1 cut(s) 844
CaiI CAGNNNCTG 1 cut(s) 947
CciI TCATGA 1 cut(s) 514
CfoI GCGC 1 cut(s) 917
Cfr13I GGNCC 1 cut(s) 1004
CseI GACGC 1 cut(s) 686
Csp6I GTAC 5 cut(s) 28, 85, 150, 395, 523
CviAII CATG 5 cut(s) 515, 693, 952, 959, 1008
CviQI GTAC 5 cut(s) 28, 85, 150, 395, 523
DdeI CTNAG 4 cut(s) 65, 99, 651, 940
DinI GGCGCC 1 cut(s) 916
DpnI GATC 5 cut(s) 129, 273, 465, 647, 1016
DpnII GATC 5 cut(s) 127, 271, 463, 645, 1014
DrdI GACNNNNNNGTC 1 cut(s) 54
DseDI GACNNNNNNGTC 1 cut(s) 54
Eam1104I CTCTTC 4 cut(s) 94, 432, 509, 584
EarI CTCTTC 4 cut(s) 94, 432, 509, 584
EciI GGCGGA 2 cut(s) 128, 293
Eco105I TACGTA 1 cut(s) 522
Eco130I CCWWGG 3 cut(s) 918, 975, 1007
Eco31I GGTCTC 1 cut(s) 917
Eco32I GATATC 1 cut(s) 163
EcoRII CCWGG 2 cut(s) 145, 479
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 3 cut(s) 918, 975, 1007
EgeI GGCGCC 1 cut(s) 916
EheI GGCGCC 1 cut(s) 916
ErhI CCWWGG 3 cut(s) 918, 975, 1007
FaeI CATG 5 cut(s) 518, 696, 955, 962, 1011
FalI AAGNNNNNCTT 2 cut(s) 73, 105
FatI CATG 5 cut(s) 514, 692, 951, 958, 1007
FauNDI CATATG 1 cut(s) 832
Fnu4HI GCNGC 2 cut(s) 656, 876
FokI GGATG 4 cut(s) 287, 483, 647, 697
Fsp4HI GCNGC 2 cut(s) 656, 876
FspBI CTAG 2 cut(s) 330, 932
GlaI GCGC 1 cut(s) 916
GluI GCNGC 2 cut(s) 656, 876
HaeII RGCGCY 1 cut(s) 918
HaeIII GGCC 1 cut(s) 1006
HapII CCGG 1 cut(s) 581
HgaI GACGC 1 cut(s) 686
HhaI GCGC 1 cut(s) 917
Hin1I GRCGYC 2 cut(s) 697, 915
Hin1II CATG 5 cut(s) 518, 696, 955, 962, 1011
Hin6I GCGC 1 cut(s) 915
HinP1I GCGC 1 cut(s) 915
HinfI GANTC 2 cut(s) 184, 476
HpaII CCGG 1 cut(s) 581
HphI GGTGA 5 cut(s) 221, 318, 355, 434, 569
Hpy166II GTNNAC 3 cut(s) 40, 363, 640
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 3 cut(s) 473, 515, 784
Hpy8I GTNNAC 3 cut(s) 40, 363, 640
HpyAV CCTTC 2 cut(s) 7, 554
HpyCH4III ACNGT 2 cut(s) 233, 293
HpyCH4IV ACGT 2 cut(s) 418, 521
HpyCH4V TGCA 1 cut(s) 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 664, 673
HpyF3I CTNAG 4 cut(s) 65, 99, 651, 940
HpySE526I ACGT 2 cut(s) 418, 521
Hsp92I GRCGYC 2 cut(s) 697, 915
Hsp92II CATG 5 cut(s) 518, 696, 955, 962, 1011
HspAI GCGC 1 cut(s) 915
KasI GGCGCC 1 cut(s) 914
Kzo9I GATC 5 cut(s) 127, 271, 463, 645, 1014
LguI GCTCTTC 2 cut(s) 432, 584
Lsp1109I GCAGC 1 cut(s) 862
LweI GCATC 1 cut(s) 667
MaeI CTAG 2 cut(s) 330, 932
MaeII ACGT 2 cut(s) 418, 521
MaeIII GTNAC 3 cut(s) 217, 311, 899
MalI GATC 5 cut(s) 129, 273, 465, 647, 1016
MboI GATC 5 cut(s) 127, 271, 463, 645, 1014
MboII GAAGA 6 cut(s) 22, 81, 449, 496, 601, 982
MflI RGATCY 1 cut(s) 645
MluCI AATT 8 cut(s) 154, 193, 244, 551, 570, 708, 788, 964
Mly113I GGCGCC 1 cut(s) 915
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 2 cut(s) 222, 612
MroXI GAANNNNTTC 1 cut(s) 111
MseI TTAA 3 cut(s) 569, 707, 711
MspI CCGG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 147, 481
MvaI CCWGG 2 cut(s) 147, 481
MwoI GCNNNNNNNGC 2 cut(s) 664, 673
NarI GGCGCC 1 cut(s) 915
NcoI CCATGG 1 cut(s) 1007
NdeI CATATG 1 cut(s) 832
NdeII GATC 5 cut(s) 127, 271, 463, 645, 1014
NlaIII CATG 5 cut(s) 518, 696, 955, 962, 1011
NlaIV GGNNCC 3 cut(s) 647, 916, 1005
NmuCI GTSAC 1 cut(s) 311
PacI TTAATTAA 1 cut(s) 711
PagI TCATGA 1 cut(s) 514
PciSI GCTCTTC 2 cut(s) 432, 584
PdmI GAANNNNTTC 1 cut(s) 111
PfeI GAWTC 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 981
PfoI TCCNGGA 1 cut(s) 479
PkrI GCNGC 2 cut(s) 657, 877
PleI GAGTC 1 cut(s) 178
PluTI GGCGCC 1 cut(s) 918
PpsI GAGTC 1 cut(s) 178
Ppu21I YACGTR 1 cut(s) 522
PsiI TTATAA 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 145, 479
PspGI CCWGG 2 cut(s) 145, 479
PspN4I GGNNCC 3 cut(s) 647, 916, 1005
PspPI GGNCC 1 cut(s) 1004
PstNI CAGNNNCTG 1 cut(s) 947
PsuI RGATCY 1 cut(s) 645
RsaI GTAC 5 cut(s) 29, 86, 151, 396, 524
RsaNI GTAC 5 cut(s) 28, 85, 150, 395, 523
SapI GCTCTTC 2 cut(s) 432, 584
SaqAI TTAA 3 cut(s) 569, 707, 711
SatI GCNGC 2 cut(s) 656, 876
Sau3AI GATC 5 cut(s) 127, 271, 463, 645, 1014
Sau96I GGNCC 1 cut(s) 1004
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 2 cut(s) 147, 481
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 1 cut(s) 354
SfoI GGCGCC 1 cut(s) 916
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
SnaBI TACGTA 1 cut(s) 522
Sse9I AATT 8 cut(s) 154, 193, 244, 551, 570, 708, 788, 964
SsiI CCGC 4 cut(s) 139, 304, 656, 878
SspDI GGCGCC 1 cut(s) 914
SspMI CTAG 2 cut(s) 330, 932
StyD4I CCNGG 2 cut(s) 145, 479
StyI CCWWGG 3 cut(s) 918, 975, 1007
TaaI ACNGT 2 cut(s) 233, 293
TaiI ACGT 2 cut(s) 421, 524
TaqI TCGA 4 cut(s) 5, 369, 817, 1017
TaqII GACCGA 1 cut(s) 60
TasI AATT 8 cut(s) 154, 193, 244, 551, 570, 708, 788, 964
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 658
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 3 cut(s) 569, 707, 711
Tru9I TTAA 3 cut(s) 569, 707, 711
TscAI CASTG 2 cut(s) 283, 816
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 1 cut(s) 875
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 5 cut(s) 23, 396, 564, 612, 681
TspRI CASTG 2 cut(s) 283, 816
Van91I CCANNNNNTGG 1 cut(s) 981
XapI RAATTY 3 cut(s) 154, 551, 788
XcmI CCANNNNNNNNNTGG 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 111
XspI CTAG 2 cut(s) 330, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.