pycom15g04240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000563
Physical Location & Seq
Forward (+)
2556059 .. 2558151
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04240.1

Sequence Viewer

Length: 1203 bp
ATGTCGAACAAGGATTGCTATTTTTACGTGCAGTTTCTGAAGCGAGTTTGCAAACTTAACATCAGTCAACTGTTACTATTCAAAAAGGGATCTACTTCTAAGAGGCCAGAATTTGAAGTTGTGCTTGCCAACCAAGATGAAAAATTTCCCCTCTGGATGGGAATACTTATCAAGGGTTCCAATATGCATTTTGTTGGGGGTGACAAGTATTTCAGGTCTGAGAATTTTTCTTACTCTGAGTTGTGCCTGTGGACTCTGCCCAACTTAGAGGTCTACACCCTTGACCCTACATCCCAAACCCTCTATAAGGACAGTAACTTTCCCAACCTTTCTGGTCCCAAGTGCACACCTATCGTGGTCACCCTTGGGGATGAAAACAAAGCATATGTCCTTTCAACAAATGCAAACCACTTTTCTGATATAGCTGAACCACCTTTCGAGGTTTTTGATTTTGTTTCAAAAACCTGGGAAGCTCTCCCTCCCCCCCCTGACCACCGTGGCCATATCATTTCCCACTATGCTATTGGGCGCAAGCTGTATGTTAATGCCGAGCTATTTTCATTCGTTTTTTATGCAGGCGAAGCCATATGGCACCGTGACATCCCATTTAAGTTTCCCCATCTTGCATGCTCTCTAGAGTATAATGGCTTTCTAATTGCCCAGCCCTTCAACTTAAGAGGTCTTGTTGCCTATAACTTGGACTCATTTGGGATCAAAAGTCCTTATCAGTGTCTAGATCAGCTACAAGAAGTATTCGAGGATCGCTTTTACGACTCCGATTGCTCTCTCACCCGTCTTGACGATAATGGCCGCATGTGCCTCCTTTATCAAGGCATGCCTTTCAGCTATTCCCTTAGGTTGTGTGCACGTGTAGCGGTGTTTCAAGTATTTGTCTCAAACTCTACCGACAATCCAGTTGTCACTGCCGTTCTAGAGGCTGTCGAAGAGTATGATATCCAAGACTTTACACTTGGTGATTACGGTGCTTATTCTACATTCATCATGTATGATGGTCTGAACAAGGACGATACTGAGGCTGATGTCACAAACTCAACTCTTTCTGGCTTGGACAGTCGCAGAGGCGTGGGCAAGCATGCCGCGGAGTTTGTCACTGATGCTGATATGCATAAAAAACCAAAAACTACACTTTATTTGGTAATGCAGTGTCACGGGACACGGTTTCTGGGCATGGCATGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

401

Amino Acids

45.63

Weight (kDa)

5.32

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 95 - 183 9.2e-06 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 591
AccI GTMKAC 1 cut(s) 273
AccII CGCG 1 cut(s) 1100
AciI CCGC 4 cut(s) 811, 875, 1098, 1100
AclWI GGATC 3 cut(s) 97, 719, 768
AcoI YGGCCR 2 cut(s) 499, 808
AcsI RAATTY 3 cut(s) 110, 143, 223
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 869
AdeI CACNNNGTG 1 cut(s) 974
AfiI CCNNNNNNNGG 2 cut(s) 157, 307
AflII CTTAAG 1 cut(s) 673
AflIII ACRYGT 1 cut(s) 868
AgsI TTSAA 6 cut(s) 82, 116, 396, 459, 670, 884
AjnI CCWGG 1 cut(s) 464
AluBI AGCT 6 cut(s) 425, 473, 535, 553, 742, 846
AluI AGCT 6 cut(s) 425, 473, 535, 553, 742, 846
Alw21I GWGCWC 2 cut(s) 347, 868
Alw26I GTCTC 1 cut(s) 898
Alw44I GTGCAC 2 cut(s) 343, 864
AlwI GGATC 3 cut(s) 97, 719, 768
AlwNI CAGNNNCTG 1 cut(s) 37
AoxI GGCC 3 cut(s) 104, 499, 808
ApaLI GTGCAC 2 cut(s) 343, 864
ApoI RAATTY 3 cut(s) 110, 143, 223
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
Asp700I GAANNNNTTC 1 cut(s) 144
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 4 cut(s) 212, 352, 781, 986
AvaII GGWCC 1 cut(s) 335
AxyI CCTNAGG 1 cut(s) 854
BaeGI GKGCMC 2 cut(s) 347, 868
BalI TGGCCA 1 cut(s) 501
BanI GGYRCC 1 cut(s) 591
BbrPI CACGTG 1 cut(s) 869
Bbv12I GWGCWC 2 cut(s) 347, 868
BccI CCATC 3 cut(s) 151, 627, 1004
BceAI ACGGC 1 cut(s) 911
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 1 cut(s) 466
BcoDI GTCTC 1 cut(s) 898
BfaI CTAG 3 cut(s) 635, 734, 932
BfrI CTTAAG 1 cut(s) 673
BisI GCNGC 2 cut(s) 811, 1098
BlsI GCNGC 2 cut(s) 812, 1099
Bme1390I CCNGG 1 cut(s) 466
Bme18I GGWCC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 3 cut(s) 178, 337, 593
BmrFI CCNGG 1 cut(s) 466
BmsI GCATC 1 cut(s) 1105
BsaAI YACGTR 2 cut(s) 28, 869
BsaJI CCNNGG 4 cut(s) 364, 465, 496, 1098
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 307
Bse1I ACTGG 1 cut(s) 914
Bse21I CCTNAGG 1 cut(s) 854
BseBI CCWGG 1 cut(s) 466
BseDI CCNNGG 4 cut(s) 364, 465, 496, 1098
BseGI GGATG 4 cut(s) 162, 290, 376, 600
BseLI CCNNNNNNNGG 2 cut(s) 157, 307
BseMII CTCAG 3 cut(s) 210, 228, 1023
BseNI ACTGG 1 cut(s) 914
BseSI GKGCMC 2 cut(s) 347, 868
BseYI CCCAGC 1 cut(s) 660
BsgI GTGCAG 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 1100
BshFI GGCC 3 cut(s) 106, 501, 810
BshNI GGYRCC 1 cut(s) 591
BsiHKAI GWGCWC 2 cut(s) 347, 868
BslFI GGGAC 2 cut(s) 321, 1186
BslI CCNNNNNNNGG 2 cut(s) 157, 307
BsmAI GTCTC 1 cut(s) 898
BsmFI GGGAC 2 cut(s) 321, 1186
BsnI GGCC 3 cut(s) 106, 501, 810
Bsp1286I GDGCHC 2 cut(s) 347, 868
Bsp143I GATC 4 cut(s) 89, 711, 736, 760
BspACI CCGC 4 cut(s) 811, 875, 1098, 1100
BspANI GGCC 3 cut(s) 106, 501, 810
BspCNI CTCAG 3 cut(s) 211, 229, 1024
BspFNI CGCG 1 cut(s) 1100
BspLI GGNNCC 3 cut(s) 178, 337, 593
BspPI GGATC 3 cut(s) 97, 719, 768
BspT107I GGYRCC 1 cut(s) 591
BspTI CTTAAG 1 cut(s) 673
BsrI ACTGG 1 cut(s) 914
BssECI CCNNGG 4 cut(s) 364, 465, 496, 1098
BssMI GATC 4 cut(s) 89, 711, 736, 760
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 466
Bst4CI ACNGT 7 cut(s) 72, 314, 497, 596, 983, 1073, 1179
Bst6I CTCTTC 1 cut(s) 939
BstAFI CTTAAG 1 cut(s) 673
BstBAI YACGTR 2 cut(s) 28, 869
BstC8I GCNNGC 7 cut(s) 126, 533, 577, 628, 836, 1091, 1095
BstDEI CTNAG 6 cut(s) 99, 219, 237, 265, 854, 1032
BstDSI CCRYGG 2 cut(s) 496, 1098
BstEII GGTNACC 1 cut(s) 358
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 4 cut(s) 162, 290, 376, 600
BstFNI CGCG 1 cut(s) 1100
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 4 cut(s) 92, 714, 739, 763
BstMAI GTCTC 1 cut(s) 898
BstMBI GATC 4 cut(s) 89, 711, 736, 760
BstMWI GCNNNNNNNGC 3 cut(s) 581, 816, 872
BstNI CCWGG 1 cut(s) 466
BstNSI RCATGY 4 cut(s) 630, 817, 838, 1097
BstPI GGTNACC 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 464
BstSLI GKGCMC 2 cut(s) 347, 868
BstUI CGCG 1 cut(s) 1100
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
Bsu36I CCTNAGG 1 cut(s) 854
BsuRI GGCC 3 cut(s) 106, 501, 810
BtgI CCRYGG 2 cut(s) 496, 1098
BtsCI GGATG 4 cut(s) 162, 290, 376, 600
BtsI GCAGTG 2 cut(s) 921, 1169
BtsIMutI CAGTG 4 cut(s) 734, 921, 1110, 1169
Cac8I GCNNGC 7 cut(s) 126, 533, 577, 628, 836, 1091, 1095
CaiI CAGNNNCTG 1 cut(s) 37
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 335
Cfr42I CCGCGG 1 cut(s) 1101
CviAII CATG 7 cut(s) 627, 814, 835, 1003, 1094, 1189, 1194
DdeI CTNAG 6 cut(s) 99, 219, 237, 265, 854, 1032
DpnI GATC 4 cut(s) 91, 713, 738, 762
DpnII GATC 4 cut(s) 89, 711, 736, 760
DraIII CACNNNGTG 1 cut(s) 974
EaeI YGGCCR 2 cut(s) 499, 808
Eam1104I CTCTTC 1 cut(s) 939
EarI CTCTTC 1 cut(s) 939
Eco130I CCWWGG 1 cut(s) 364
Eco32I GATATC 1 cut(s) 955
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 869
Eco81I CCTNAGG 1 cut(s) 854
Eco91I GGTNACC 1 cut(s) 358
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 464
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 1 cut(s) 364
EcoT22I ATGCAT 2 cut(s) 189, 1128
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 7 cut(s) 630, 817, 838, 1006, 1097, 1192, 1197
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 2 cut(s) 321, 1186
FatI CATG 7 cut(s) 626, 813, 834, 1002, 1093, 1188, 1193
FauNDI CATATG 2 cut(s) 385, 587
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 2 cut(s) 811, 1098
FokI GGATG 4 cut(s) 169, 277, 383, 587
Fsp4HI GCNGC 2 cut(s) 811, 1098
FspBI CTAG 3 cut(s) 635, 734, 932
GlaI GCGC 1 cut(s) 530
GluI GCNGC 2 cut(s) 811, 1098
GsaI CCCAGC 1 cut(s) 664
HaeIII GGCC 3 cut(s) 106, 501, 810
HhaI GCGC 1 cut(s) 531
Hin1II CATG 7 cut(s) 630, 817, 838, 1006, 1097, 1192, 1197
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 68
HindII GTYRAC 1 cut(s) 68
HinfI GANTC 3 cut(s) 253, 701, 773
HphI GGTGA 4 cut(s) 212, 352, 781, 986
Hpy166II GTNNAC 5 cut(s) 68, 252, 274, 345, 866
Hpy188I TCNGA 6 cut(s) 39, 220, 238, 418, 778, 1017
Hpy188III TCNNGA 5 cut(s) 154, 635, 734, 797, 932
Hpy8I GTNNAC 5 cut(s) 68, 252, 274, 345, 866
HpyAV CCTTC 1 cut(s) 676
HpyCH4III ACNGT 7 cut(s) 72, 314, 497, 596, 983, 1073, 1179
HpyCH4IV ACGT 2 cut(s) 27, 868
HpyF10VI GCNNNNNNNGC 3 cut(s) 581, 816, 872
HpyF3I CTNAG 6 cut(s) 99, 219, 237, 265, 854, 1032
HpySE526I ACGT 2 cut(s) 27, 868
Hsp92II CATG 7 cut(s) 630, 817, 838, 1006, 1097, 1192, 1197
HspAI GCGC 1 cut(s) 529
KspI CCGCGG 1 cut(s) 1101
Kzo9I GATC 4 cut(s) 89, 711, 736, 760
LweI GCATC 1 cut(s) 1105
MaeI CTAG 3 cut(s) 635, 734, 932
MaeII ACGT 2 cut(s) 27, 868
MaeIII GTNAC 9 cut(s) 72, 200, 314, 358, 596, 919, 1042, 1108, 1166
MalI GATC 4 cut(s) 91, 713, 738, 762
MboI GATC 4 cut(s) 89, 711, 736, 760
MboII GAAGA 1 cut(s) 956
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 2 cut(s) 347, 868
MlsI TGGCCA 1 cut(s) 501
MluCI AATT 4 cut(s) 110, 143, 223, 654
MluNI TGGCCA 1 cut(s) 501
MlyI GAGTC 3 cut(s) 247, 695, 767
Mox20I TGGCCA 1 cut(s) 501
Mph1103I ATGCAT 2 cut(s) 189, 1128
MroXI GAANNNNTTC 1 cut(s) 144
MscI TGGCCA 1 cut(s) 501
MseI TTAA 4 cut(s) 57, 543, 609, 674
Msp20I TGGCCA 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 1100
MspCI CTTAAG 1 cut(s) 673
MspR9I CCNGG 1 cut(s) 466
MvaI CCWGG 1 cut(s) 466
MvnI CGCG 1 cut(s) 1100
MwoI GCNNNNNNNGC 3 cut(s) 581, 816, 872
NdeI CATATG 2 cut(s) 385, 587
NdeII GATC 4 cut(s) 89, 711, 736, 760
NlaIII CATG 7 cut(s) 630, 817, 838, 1006, 1097, 1192, 1197
NlaIV GGNNCC 3 cut(s) 178, 337, 593
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 7 cut(s) 200, 358, 596, 919, 1042, 1108, 1166
NsiI ATGCAT 2 cut(s) 189, 1128
NspI RCATGY 4 cut(s) 630, 817, 838, 1097
PaeI GCATGC 3 cut(s) 630, 838, 1097
PdmI GAANNNNTTC 1 cut(s) 144
PkrI GCNGC 2 cut(s) 812, 1099
PleI GAGTC 3 cut(s) 247, 695, 767
PmaCI CACGTG 1 cut(s) 869
PmlI CACGTG 1 cut(s) 869
PpsI GAGTC 3 cut(s) 247, 695, 767
Ppu21I YACGTR 2 cut(s) 28, 869
Psp6I CCWGG 1 cut(s) 464
PspCI CACGTG 1 cut(s) 869
PspEI GGTNACC 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 660
PspGI CCWGG 1 cut(s) 464
PspN4I GGNNCC 3 cut(s) 178, 337, 593
PspPI GGNCC 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 89
SacII CCGCGG 1 cut(s) 1101
SaqAI TTAA 4 cut(s) 57, 543, 609, 674
SatI GCNGC 2 cut(s) 811, 1098
Sau3AI GATC 4 cut(s) 89, 711, 736, 760
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 3 cut(s) 247, 695, 767
ScrFI CCNGG 1 cut(s) 466
SduI GDGCHC 2 cut(s) 347, 868
SfaNI GCATC 1 cut(s) 1105
Sfr303I CCGCGG 1 cut(s) 1101
SgrBI CCGCGG 1 cut(s) 1101
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 673
SmoI CTYRAG 1 cut(s) 673
SphI GCATGC 3 cut(s) 630, 838, 1097
Sse9I AATT 4 cut(s) 110, 143, 223, 654
SsiI CCGC 4 cut(s) 811, 875, 1098, 1100
SspMI CTAG 3 cut(s) 635, 734, 932
StyD4I CCNGG 1 cut(s) 464
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 7 cut(s) 72, 314, 497, 596, 983, 1073, 1179
TaiI ACGT 2 cut(s) 30, 871
TaqI TCGA 4 cut(s) 5, 438, 756, 942
TasI AATT 4 cut(s) 110, 143, 223, 654
TauI GCSGC 2 cut(s) 813, 1100
Tru1I TTAA 4 cut(s) 57, 543, 609, 674
Tru9I TTAA 4 cut(s) 57, 543, 609, 674
TscAI CASTG 4 cut(s) 734, 928, 1117, 1169
TseFI GTSAC 7 cut(s) 200, 358, 596, 919, 1042, 1108, 1166
Tsp45I GTSAC 7 cut(s) 200, 358, 596, 919, 1042, 1108, 1166
TspDTI ATGAA 4 cut(s) 153, 387, 549, 988
TspRI CASTG 4 cut(s) 734, 928, 1117, 1169
Vha464I CTTAAG 1 cut(s) 673
VneI GTGCAC 2 cut(s) 343, 864
VpaK11BI GGWCC 1 cut(s) 335
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 3 cut(s) 110, 143, 223
XbaI TCTAGA 3 cut(s) 634, 733, 931
XceI RCATGY 4 cut(s) 630, 817, 838, 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 273
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 3 cut(s) 635, 734, 932
Zsp2I ATGCAT 2 cut(s) 189, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.