MD15G1401000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000563
Physical Location & Seq
Reverse (-)
50189670 .. 50192458
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1401000.v1.1.491

Sequence Viewer

Length: 894 bp
ATGTTGTATCCTATGTGCAAGTCTAACCTTCCCCGCATGTGTGGTCCGAAGCTCCAACCCTTCGTGGCCACCATTGACGACAAGATTTATGTCTTGTCTACAGAGTACAGAGGAAAGGACGTCCAAGAAACCTCACCCGCCCTGTTTGAGGTCTTCCATCCTTCCCTTCTTCTTTGGAAAGTTCTACCGAATCCTCCATTCATCGTTGATGTTAAGATTGAGGGTGTATTCATGGATTGCTGCTACACATGGGATAAGAATCTCATTGTTCGAGACCGGATGACTGGCCAACGCTATCAGTTTGACACTAGTTCAGAAAAATGGCACGAAGATTGTGGCATCATGGGCGGAATGTATGCCCGGGGAGGTTTAGCACAGTATAAGGATTTTCTCCTTTTTCCCCAACTAGGGTGTGTCTGTAGTTATCGATCGGGTTGCGGTGGCCTTTCCTTCGACTCTCAGGAGGTGGATGGGCTCCGGAAGATATTCCGTCCCCATATAAACAACTGCACTTCTTTCATCACCCAGTTTGGCGAGGAAGACCTTATGTGCATCGTGTGCTCCGGCTTTGACAAGCTGGGTTTCTGCTGTTGCCGTGTCGCAGTCTTCAAGGTGTCGATCGAGGGCTTTCCTGAGAATCCATCCATTCGTGCTGATGTTGTAGCCGAAGCAACATACTATTTCAGTGATGAAGTCATTCCCGACGAGAAAATCTTCCAAGTGTTTGCCATGCGTGATTATGCAAACAAGCTTGATGACTTGGTCGATGCAAGTTGTTTGAAGGAGGATCTTCCTGGGTTGGATTGGGCGTTGGCTGCTGTGAAGGGGCGGGGAAGAAAGCGCGAGGAGGAGGCGGTGCTGGAAACTAGGAGCCAAAGGAGGAGAGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

33.87

Weight (kDa)

5.21

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 9 - 110 4.9e-09 Protein of unknown function (DUF1668)
Kelch_FKB95 PF25210 9 - 112 3.4e-06 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 98
AccII CGCG 1 cut(s) 843
AccIII TCCGGA 1 cut(s) 477
AciI CCGC 6 cut(s) 34, 138, 348, 438, 829, 854
AclWI GGATC 1 cut(s) 795
AcoI YGGCCR 2 cut(s) 66, 286
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 3 cut(s) 148, 407, 408
AgsI TTSAA 2 cut(s) 610, 781
AhlI ACTAGT 1 cut(s) 308
AjnI CCWGG 1 cut(s) 793
AluBI AGCT 3 cut(s) 52, 577, 751
AluI AGCT 3 cut(s) 52, 577, 751
Alw21I GWGCWC 1 cut(s) 563
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 1 cut(s) 795
Ama87I CYCGRG 1 cut(s) 360
Aor13HI TCCGGA 1 cut(s) 477
AoxI GGCC 3 cut(s) 66, 286, 442
ApeKI GCWGC 2 cut(s) 240, 815
Asp700I GAANNNNTTC 3 cut(s) 485, 696, 713
AspLEI GCGC 1 cut(s) 843
AspS9I GGNCC 1 cut(s) 44
AsuC2I CCSGG 2 cut(s) 361, 362
AsuHPI GGTGA 2 cut(s) 126, 514
AvaI CYCGRG 1 cut(s) 360
AvaII GGWCC 1 cut(s) 44
BalI TGGCCA 2 cut(s) 68, 288
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 3 cut(s) 145, 546, 598
Bbv12I GWGCWC 1 cut(s) 563
BbvI GCAGC 2 cut(s) 227, 802
BccI CCATC 3 cut(s) 165, 464, 649
BceAI ACGGC 1 cut(s) 579
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BciT130I CCWGG 1 cut(s) 795
BciVI GTATCC 1 cut(s) 18
BcnI CCSGG 2 cut(s) 361, 362
BcoDI GTCTC 1 cut(s) 267
BcuI ACTAGT 1 cut(s) 308
BfaI CTAG 3 cut(s) 309, 407, 867
BfmI CTRYAG 2 cut(s) 99, 418
BfuI GTATCC 1 cut(s) 18
BisI GCNGC 2 cut(s) 241, 816
BlsI GCNGC 2 cut(s) 242, 817
Bme1390I CCNGG 3 cut(s) 361, 362, 795
Bme18I GGWCC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 476, 872
BmrFI CCNGG 3 cut(s) 361, 362, 795
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 3 cut(s) 348, 561, 757
BmuI ACTGGG 1 cut(s) 520
BpiI GAAGAC 3 cut(s) 145, 546, 598
BpuMI CCSGG 2 cut(s) 361, 362
Bsa29I ATCGAT 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 360, 361, 794
BsaWI WCCGGW 2 cut(s) 276, 477
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 407, 408
Bse1I ACTGG 2 cut(s) 289, 526
Bse8I GATNNNNATC 1 cut(s) 258
BseAI TCCGGA 1 cut(s) 477
BseBI CCWGG 1 cut(s) 795
BseCI ATCGAT 1 cut(s) 427
BseDI CCNNGG 3 cut(s) 360, 361, 794
BseGI GGATG 4 cut(s) 157, 285, 475, 641
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 148, 407, 408
BseMII CTCAG 2 cut(s) 473, 624
BseNI ACTGG 2 cut(s) 289, 526
BseRI GAGGAG 2 cut(s) 860, 863
BseXI GCAGC 2 cut(s) 227, 802
BseYI CCCAGC 1 cut(s) 577
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 1 cut(s) 843
Bsh1285I CGRYCG 2 cut(s) 431, 621
BshFI GGCC 3 cut(s) 68, 288, 444
BshVI ATCGAT 1 cut(s) 427
BsiEI CGRYCG 2 cut(s) 431, 621
BsiHKAI GWGCWC 1 cut(s) 563
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 4 cut(s) 277, 361, 478, 564
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 3 cut(s) 148, 407, 408
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 477
BsnI GGCC 3 cut(s) 68, 288, 444
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 360
Bsp1286I GDGCHC 2 cut(s) 477, 563
Bsp13I TCCGGA 1 cut(s) 477
Bsp143I GATC 3 cut(s) 428, 618, 787
BspACI CCGC 6 cut(s) 34, 138, 348, 438, 829, 854
BspANI GGCC 3 cut(s) 68, 288, 444
BspCNI CTCAG 2 cut(s) 472, 625
BspDI ATCGAT 1 cut(s) 427
BspEI TCCGGA 1 cut(s) 477
BspFNI CGCG 1 cut(s) 843
BspLI GGNNCC 2 cut(s) 476, 872
BspPI GGATC 1 cut(s) 795
BspTNI GGTCTC 1 cut(s) 267
BsrI ACTGG 2 cut(s) 289, 526
BssECI CCNNGG 3 cut(s) 360, 361, 794
BssMI GATC 3 cut(s) 428, 618, 787
BssNI GRCGYC 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 459, 633
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 4 cut(s) 157, 285, 475, 641
BstFNI CGCG 1 cut(s) 843
BstHHI GCGC 1 cut(s) 843
BstKTI GATC 3 cut(s) 431, 621, 790
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 3 cut(s) 428, 618, 787
BstMCI CGRYCG 2 cut(s) 431, 621
BstMWI GCNNNNNNNGC 3 cut(s) 345, 558, 815
BstNI CCWGG 1 cut(s) 795
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 3 cut(s) 359, 360, 793
BstSFI CTRYAG 2 cut(s) 99, 418
BstUI CGCG 1 cut(s) 843
BstV1I GCAGC 2 cut(s) 227, 802
BstV2I GAAGAC 3 cut(s) 145, 546, 598
BstX2I RGATCY 1 cut(s) 787
BstYI RGATCY 1 cut(s) 787
Bsu15I ATCGAT 1 cut(s) 427
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 3 cut(s) 68, 288, 444
BsuTUI ATCGAT 1 cut(s) 427
BtsCI GGATG 4 cut(s) 157, 285, 475, 641
BtsIMutI CAGTG 1 cut(s) 691
CfoI GCGC 1 cut(s) 843
Cfr13I GGNCC 1 cut(s) 44
Cfr9I CCCGGG 1 cut(s) 360
ClaI ATCGAT 1 cut(s) 427
Csp6I GTAC 1 cut(s) 106
CviAII CATG 5 cut(s) 37, 232, 249, 343, 730
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 2 cut(s) 459, 633
DpnI GATC 3 cut(s) 430, 620, 789
DpnII GATC 3 cut(s) 428, 618, 787
EaeI YGGCCR 2 cut(s) 66, 286
EciI GGCGGA 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 477
Eco31I GGTCTC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 360
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 793
EcoT38I GRGCYC 1 cut(s) 477
FaeI CATG 5 cut(s) 40, 235, 252, 346, 733
FaqI GGGAC 1 cut(s) 477
FatI CATG 5 cut(s) 36, 231, 248, 342, 729
FauI CCCGC 3 cut(s) 41, 145, 822
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 2 cut(s) 241, 816
FokI GGATG 4 cut(s) 144, 292, 482, 628
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 2 cut(s) 241, 816
FspBI CTAG 3 cut(s) 309, 407, 867
GlaI GCGC 1 cut(s) 842
GluI GCNGC 2 cut(s) 241, 816
GsaI CCCAGC 1 cut(s) 581
HaeIII GGCC 3 cut(s) 68, 288, 444
HapII CCGG 4 cut(s) 277, 361, 478, 564
HhaI GCGC 1 cut(s) 843
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 5 cut(s) 40, 235, 252, 346, 733
Hin6I GCGC 1 cut(s) 841
HinP1I GCGC 1 cut(s) 841
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 4 cut(s) 190, 259, 455, 637
HpaII CCGG 4 cut(s) 277, 361, 478, 564
HphI GGTGA 2 cut(s) 126, 514
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 2 cut(s) 48, 316
Hpy188III TCNNGA 5 cut(s) 272, 461, 478, 632, 701
Hpy8I GTNNAC 1 cut(s) 99
Hpy99I CGWCG 1 cut(s) 707
HpyAV CCTTC 7 cut(s) 38, 70, 171, 176, 460, 775, 817
HpyCH4III ACNGT 1 cut(s) 378
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 5 cut(s) 18, 510, 552, 743, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 345, 558, 815
HpyF3I CTNAG 2 cut(s) 459, 633
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 5 cut(s) 40, 235, 252, 346, 733
HspAI GCGC 1 cut(s) 841
Kpn2I TCCGGA 1 cut(s) 477
Kzo9I GATC 3 cut(s) 428, 618, 787
LmnI GCTCC 4 cut(s) 57, 480, 566, 870
Lsp1109I GCAGC 2 cut(s) 227, 802
LweI GCATC 3 cut(s) 348, 561, 757
MaeI CTAG 3 cut(s) 309, 407, 867
MaeII ACGT 1 cut(s) 120
MalI GATC 3 cut(s) 430, 620, 789
MboI GATC 3 cut(s) 428, 618, 787
MboII GAAGA 9 cut(s) 145, 161, 341, 493, 551, 598, 706, 782, 846
MflI RGATCY 1 cut(s) 787
MhlI GDGCHC 2 cut(s) 477, 563
MlsI TGGCCA 2 cut(s) 68, 288
MluNI TGGCCA 2 cut(s) 68, 288
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 79, 780
Mox20I TGGCCA 2 cut(s) 68, 288
MroI TCCGGA 1 cut(s) 477
MroXI GAANNNNTTC 3 cut(s) 485, 696, 713
MscI TGGCCA 2 cut(s) 68, 288
MseI TTAA 1 cut(s) 213
Msp20I TGGCCA 2 cut(s) 68, 288
MspI CCGG 4 cut(s) 277, 361, 478, 564
MspR9I CCNGG 3 cut(s) 361, 362, 795
MvaI CCWGG 1 cut(s) 795
MvnI CGCG 1 cut(s) 843
MwoI GCNNNNNNNGC 3 cut(s) 345, 558, 815
NciI CCSGG 2 cut(s) 361, 362
NdeII GATC 3 cut(s) 428, 618, 787
NlaIII CATG 5 cut(s) 40, 235, 252, 346, 733
NlaIV GGNNCC 2 cut(s) 476, 872
NspI RCATGY 1 cut(s) 40
PdmI GAANNNNTTC 3 cut(s) 485, 696, 713
PfeI GAWTC 3 cut(s) 190, 259, 637
PflFI GACNNNGTC 1 cut(s) 761
PkrI GCNGC 2 cut(s) 242, 817
Ple19I CGATCG 2 cut(s) 431, 621
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 793
PspFI CCCAGC 1 cut(s) 577
PspGI CCWGG 1 cut(s) 793
PspN4I GGNNCC 2 cut(s) 476, 872
PspPI GGNCC 1 cut(s) 44
PsuI RGATCY 1 cut(s) 787
PsyI GACNNNGTC 1 cut(s) 761
PvuI CGATCG 2 cut(s) 431, 621
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 241, 816
Sau3AI GATC 3 cut(s) 428, 618, 787
Sau96I GGNCC 1 cut(s) 44
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 361, 362, 795
SduI GDGCHC 2 cut(s) 477, 563
SfaNI GCATC 3 cut(s) 348, 561, 757
SfcI CTRYAG 2 cut(s) 99, 418
SinI GGWCC 1 cut(s) 44
SmaI CCCGGG 1 cut(s) 362
SpeI ACTAGT 1 cut(s) 308
SsiI CCGC 6 cut(s) 34, 138, 348, 438, 829, 854
SspMI CTAG 3 cut(s) 309, 407, 867
StyD4I CCNGG 3 cut(s) 359, 360, 793
TaaI ACNGT 1 cut(s) 378
TaiI ACGT 1 cut(s) 123
TaqI TCGA 6 cut(s) 271, 427, 453, 617, 621, 765
TatI WGTACW 1 cut(s) 105
TfiI GAWTC 3 cut(s) 190, 259, 637
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 691
TseI GCWGC 2 cut(s) 240, 815
TspDTI ATGAA 4 cut(s) 190, 220, 508, 705
TspGWI ACGGA 1 cut(s) 479
TspMI CCCGGG 1 cut(s) 360
TspRI CASTG 1 cut(s) 691
Tth111I GACNNNGTC 1 cut(s) 761
VpaK11BI GGWCC 1 cut(s) 44
XagI CCTNNNNNAGG 1 cut(s) 146
XceI RCATGY 1 cut(s) 40
XmaI CCCGGG 1 cut(s) 360
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 3 cut(s) 485, 696, 713
XspI CTAG 3 cut(s) 309, 407, 867
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.