Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0000408

Physical Location & Seq

Genomic Coordinates Reverse (-)

6377321 .. 6380886

Gene Structure 3566 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g11250.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGTCGACTGTTGCTCTCAACTTTGTTGGAGGTATACCTTTCCAACTACTGTATGATGGCATTAAGATAGCCACTCGCAGGACTAGCAAGTTTAGAAGCAACCTCATAAGCCTCCAGTTCACACTTGACTGTTTACAATCACGGATTATTGAACAGATAGGAGATAATAACGTTGAACTGAATCTCCCCAACAACATAATAGAAGGTATTCAAACAAAAATGAATGAGGGTGTAGTACTAGTGGCCATGTTATCGGAGCTTAGAGCGTGGAACATCCGCATCTGGGGCAATTGCTACAACTGCATAGGGCCTAATTGCGCGGATCAACTTGATGAATTGGATAGGTCTCTTAGAAGCCTGTTAGATCAATTAGAGTTAGAGCAAAGGAGGAATGTGACCCGGCTCATGATTTTGGCAATAAACGCCAGAGATAAACAAGATGAGTTGGAAAAAAGACAGTTGGATATTCTGAAAGGGCAGCAACAAATACTTGGTATGTTGATGGAACAGCATCACGCGATAGGGAAAATTGTATCAAGTAGTGTGCAGGTTGCATCGTCAACCGGAGGAGGAGGAGGGACTGCTCCTGCTCTAGGACTAGTATTCCGGCTGCTTTTTGATGCTGTTACGCAAGTGAGGGTTAAGAATGAGATGGTTAAACGTCTCCTTAGAAGATTCAAATCGACACTAAATTACTTACAGCCACTGATAGAAGAGGTAGCCAAATCTAAGAGAGTATTGCATCTTTCGGAGCAAGAAGTAGAAATGTTTATTTCAGAAGTAGAGAATGGAGTAGAGCTCATTCACAAATGCTCTGAAGTTAGCAAGCGGGTCAACTACAAAAAGTACAAATACACTAATAAAATTCTAGATTTGGATAAATCTCTTCAAAGGCTCTTCTTTGAATTGAAAGGGCAGGTGGAAAGAAATCTGAAAGAGGCCATGGAGTCAGCAAATACAATAGAGGCTGTGATCAAGAAAATTGAAGGGGGTGCTGACCAACAAAAGAGTGAACTTAAAAGTGAAAGTCAGAGTTCAGTACCTCAACCTGGATCATTTGCAATTGGATTGGACATACTCAATGAGCAGGGACATAGGGATATGAACAAGGACACACAGGTGGACTCAACAAGGAATGTAGAGGTGGTGAGGCCTATTGAAAAGAGTGATGCAGTAGAACATCAAATGGAAACTAAAAGTTCCTGTGAAGTGCCCCCTTCACCTGCAGGTGGATTGGATGTACTGAACTTGCAGGGTTCTTTGGATATGGATTCAACTACCAGTAATATCGAGGCGGGGGTAATGGATATGGAAGGCATAGAGGTTGATGATTTAAAAACGAATGTACATGTGAAGGGGACATTAGAATTTGCAATTAATGTAGAGAAGATGGGACTCATGGGAGTCAAGAAAATTGAAGGAGGTGGTGGAAGTCAAGATATTAAAATTGCAGTAGCTGAAACTGAACCTTTATCACCTGCAGTTCAGTTGGATGTACAAGATGAAGTGCACGGAACAAGGGACGCGAAGGTGACATTGGAAATTCGGAAGAATGTTAAAGATGCAATCACAACAGCTGCTGAACCTTCATCCCCATCTGCTGAAGTTGGAGTGAAATTGGATTCTTCTGCATTCCCATCTTCATCGCCTCTGTGTACACAAGCAGAGCTCAAACTACCTGATAAAGAAAAGGTGATTGAAGAGGTTGTTCAAGTTCTAAGGCCATCTTCAAGCTTTTTGAAGATAGGGTCTTCACTGGGAATCCAAGCACTAGAGCCATCAAGCTTATCTCTGGTCTTTACCAAGAGCCTGTTTCTCCCAATCTTCACTGAAACCAAGATATCAGACGAAGACAACAACCAAATACAAATACTTGTTGTGGGCAAGAACATGATCCGTGGAAGCCACCAAATGGCTCCGATCTCTCTTCCACACCCGGTCAAACTAGAAATCGTTGTTCTTAACGGTGACTTTGGAGATCGAGAGAACTGGACGAGACAGGAATTCGACAGCAAAGTTGTGAAGGAGAGAAACGGCAAGAGGCCGCTGCTCACCGGAGACCTGAGTGTTACGTTGAGAGATGGGTTTTCGACGATCGGCGATATTAAATTTACGGACAACTCGAGTTGGATTCGGGGCAGGAAATTTAGGATTGGTGCAAGGGTGGCTCCGGGGTATCAGGGTCCGAGGATTCGGGAAGCCATGACTGATGCTTTCGTTGTCAAAGATGGTCCGGGCCTTGTGCGTGCAGCGTACAATAAGCATTATCCACCAATGTTGGAAGACGAAGTTTGGCGGTTGGAGAAGATTGGGAAATATGGAGCTTTTCACAAGAGGCTAGCTGCGGAAAACATTCATACAGTTGAAGATTTCTTGAAGATGTCTGTCGTGGACCCAGTGAAGCTTAAAAAGATATTAGGGTTTGGAATGTCGGAAAGAATGTGGGAAGCAACTCTAAAGCATGCGAGAACATGCGAGATGGGGAACAAAATGTACATTTTTCGTCATCTTCCTTTCGTGCTATTCTTAAATCCAATATGTCAAGTGGTAAAGGTTTTCATAAATGGCCAAGATTACCCTACTCGGGATCTAAGCAACAACAATATGGTACGTGAGGAAAGCGCATGCATACTGGAGTTCCTTGGAGGCGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002237 GO:0002239 GO:0002682 GO:0002831 GO:0002833 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009889 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010817 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0032350 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043900 GO:0043902 GO:0044424 GO:0044464 GO:0045088 GO:0046885 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0071216 GO:0071219 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1900424 GO:1900426 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Calmodulin_bind

Protein Analysis

885

Amino Acids

98.53

Weight (kDa)

6.2

Isoelectric Point (pI)

49.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	11 - 137	2.6e-08	Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8	PF05659	196 - 318	4e-23	Arabidopsis broad-spectrum mildew resistance protein RPW8
Calmodulin_bind	PF07887	597 - 743	6.1e-53	Calmodulin binding protein-like N-terminal domain
Calmod_bind_M	PF20451	759 - 825	2.6e-31	Calmodulin binding protein central domain
Calmod_bind_C	PF20452	830 - 869	2e-08	Calmodulin binding protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000408)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica	MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica	Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis	pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis	RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata	RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora	Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii	Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa	Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha	Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana	Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	4 cut(s)	907, 1217, 1231, 1486
Acc36I	ACCTGC	5 cut(s)	538, 907, 1217, 1231, 1486
AccB7I	CCANNNNNTGG	1 cut(s)	1912
AccI	GTMKAC	2 cut(s)	5, 34
AccII	CGCG	3 cut(s)	320, 518, 1526
AciI	CCGC	8 cut(s)	277, 320, 829, 1295, 2045, 2296, 2345, 2649
AclI	AACGTT	1 cut(s)	171
AclWI	GGATC	4 cut(s)	330, 1060, 1888, 2595
AcoI	YGGCCR	2 cut(s)	243, 2566
AcsI	RAATTY	6 cut(s)	864, 1367, 1542, 2003, 2108, 2144
AcuI	CTGAAG	2 cut(s)	837, 1623
AfiI	CCNNNNNNNGG	7 cut(s)	78, 283, 593, 1049, 1229, 1912, 2584
AflIII	ACRYGT	1 cut(s)	1348
AhlI	ACTAGT	2 cut(s)	238, 598
AjnI	CCWGG	1 cut(s)	1048
AjuI	GAANNNNNNNTTGG	2 cut(s)	1520, 1552
AleI	CACNNNNGTG	1 cut(s)	1118
AloI	GAACNNNNNNTCC	2 cut(s)	168, 200
Alw21I	GWGCWC	3 cut(s)	801, 1512, 1671
Alw26I	GTCTC	4 cut(s)	351, 668, 1990, 2052
Alw44I	GTGCAC	1 cut(s)	1508
AlwI	GGATC	4 cut(s)	330, 1060, 1888, 2595
AlwNI	CAGNNNCTG	3 cut(s)	706, 1457, 1580
Ama87I	CYCGRG	2 cut(s)	2122, 2583
AoxI	GGCC	8 cut(s)	243, 308, 939, 1151, 1721, 2042, 2236, 2566
ApaLI	GTGCAC	1 cut(s)	1508
ApeKI	GCWGC	6 cut(s)	478, 610, 1577, 2047, 2249, 2342
ApoI	RAATTY	6 cut(s)	864, 1367, 1542, 2003, 2108, 2144
AseI	ATTAAT	1 cut(s)	1377
Asp700I	GAANNNNTTC	2 cut(s)	207, 2352
AspLEI	GCGC	2 cut(s)	320, 2624
AspS9I	GGNCC	5 cut(s)	308, 2183, 2231, 2236, 2392
AsuC2I	CCSGG	4 cut(s)	400, 1937, 2172, 2235
AsuHPI	GGTGA	7 cut(s)	1159, 1212, 1467, 1543, 1705, 1979, 2044
AsuNHI	GCTAGC	1 cut(s)	2338
AvaI	CYCGRG	2 cut(s)	2122, 2583
AvaII	GGWCC	3 cut(s)	2183, 2231, 2392
BaeGI	GKGCMC	2 cut(s)	1215, 1512
BalI	TGGCCA	2 cut(s)	245, 2568
BanII	GRGCYC	2 cut(s)	801, 1671
BbsI	GAAGAC	3 cut(s)	1743, 1857, 2289
Bbv12I	GWGCWC	3 cut(s)	801, 1512, 1671
BbvI	GCAGC	6 cut(s)	490, 597, 1564, 2034, 2261, 2329
BceAI	ACGGC	1 cut(s)	2050
BciT130I	CCWGG	1 cut(s)	1050
BclI	TGATCA	1 cut(s)	972
BcnI	CCSGG	4 cut(s)	400, 1937, 2172, 2235
BcoDI	GTCTC	4 cut(s)	351, 668, 1990, 2052
BcuI	ACTAGT	2 cut(s)	238, 598
BfaI	CTAG	8 cut(s)	84, 239, 593, 599, 869, 1772, 1946, 2339
BfmI	CTRYAG	2 cut(s)	1224, 1479
BfuAI	ACCTGC	5 cut(s)	538, 907, 1217, 1231, 1486
BisI	GCNGC	8 cut(s)	479, 611, 1578, 2045, 2048, 2250, 2343, 2650
BlsI	GCNGC	8 cut(s)	480, 612, 1579, 2046, 2049, 2251, 2344, 2651
BmcAI	AGTACT	1 cut(s)	237
Bme1390I	CCNGG	5 cut(s)	400, 1050, 1937, 2172, 2235
Bme18I	GGWCC	3 cut(s)	2183, 2231, 2392
BmeT110I	CYCGRG	2 cut(s)	2122, 2583
BmgT120I	GGNCC	5 cut(s)	308, 2183, 2231, 2236, 2392
BmiI	GGNNCC	4 cut(s)	1917, 2169, 2184, 2394
BmrFI	CCNGG	5 cut(s)	400, 1050, 1937, 2172, 2235
BmrI	ACTGGG	2 cut(s)	1766, 2390
BmsI	GCATC	8 cut(s)	288, 520, 563, 610, 751, 1159, 1552, 2200
BmtI	GCTAGC	1 cut(s)	2342
BmuI	ACTGGG	2 cut(s)	1766, 2390
BpiI	GAAGAC	3 cut(s)	1743, 1857, 2289
BplI	GAGNNNNNCTC	2 cut(s)	783, 815
BpmI	CTGGAG	2 cut(s)	98, 2654
BpuMI	CCSGG	4 cut(s)	400, 1937, 2172, 2235
BsaAI	YACGTR	1 cut(s)	2612
BsaBI	GATNNNNATC	1 cut(s)	679
BsaI	GGTCTC	2 cut(s)	351, 2052
BsaJI	CCNNGG	5 cut(s)	942, 1897, 2171, 2186, 2641
BsaWI	WCCGGW	2 cut(s)	563, 2054
BsaXI	ACNNNNNCTCC	2 cut(s)	168, 198
Bsc4I	CCNNNNNNNGG	7 cut(s)	78, 283, 593, 1049, 1229, 1912, 2584
Bse1I	ACTGG	6 cut(s)	115, 1281, 1761, 1994, 2396, 2637
Bse8I	GATNNNNATC	1 cut(s)	679
BseBI	CCWGG	1 cut(s)	1050
BseDI	CCNNGG	5 cut(s)	942, 1897, 2171, 2186, 2641
BseGI	GGATG	4 cut(s)	273, 1243, 1498, 1589
BseJI	GATNNNNATC	1 cut(s)	679
BseLI	CCNNNNNNNGG	7 cut(s)	78, 283, 593, 1049, 1229, 1912, 2584
BseMII	CTCAG	1 cut(s)	2054
BseNI	ACTGG	6 cut(s)	115, 1281, 1761, 1994, 2396, 2637
BseRI	GAGGAG	3 cut(s)	582, 585, 588
BseSI	GKGCMC	2 cut(s)	1215, 1512
BseXI	GCAGC	6 cut(s)	490, 597, 1564, 2034, 2261, 2329
BsgI	GTGCAG	2 cut(s)	566, 2268
Bsh1236I	CGCG	3 cut(s)	320, 518, 1526
Bsh1285I	CGRYCG	1 cut(s)	2097
BshFI	GGCC	8 cut(s)	245, 310, 941, 1153, 1723, 2044, 2238, 2568
BsiEI	CGRYCG	1 cut(s)	2097
BsiHKAI	GWGCWC	3 cut(s)	801, 1512, 1671
BsiHKCI	CYCGRG	2 cut(s)	2122, 2583
BsiSI	CCGG	7 cut(s)	400, 564, 607, 1937, 2055, 2171, 2234
BslFI	GGGAC	5 cut(s)	592, 1104, 1372, 1407, 1535
BslI	CCNNNNNNNGG	7 cut(s)	78, 283, 593, 1049, 1229, 1912, 2584
BsmAI	GTCTC	4 cut(s)	351, 668, 1990, 2052
BsmBI	CGTCTC	1 cut(s)	668
BsmFI	GGGAC	5 cut(s)	592, 1104, 1372, 1407, 1535
BsmI	GAATGC	1 cut(s)	1631
BsnI	GGCC	8 cut(s)	245, 310, 941, 1153, 1723, 2044, 2238, 2568
Bso31I	GGTCTC	2 cut(s)	351, 2052
BsoBI	CYCGRG	2 cut(s)	2122, 2583
Bsp1286I	GDGCHC	4 cut(s)	801, 1215, 1512, 1671
Bsp1407I	TGTACA	4 cut(s)	1345, 1495, 1655, 2493
Bsp143I	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
Bsp19I	CCATGG	1 cut(s)	942
BspACI	CCGC	8 cut(s)	277, 320, 829, 1295, 2045, 2296, 2345, 2649
BspANI	GGCC	8 cut(s)	245, 310, 941, 1153, 1723, 2044, 2238, 2568
BspCNI	CTCAG	1 cut(s)	2055
BspFNI	CGCG	3 cut(s)	320, 518, 1526
BspHI	TCATGA	1 cut(s)	405
BspLI	GGNNCC	4 cut(s)	1917, 2169, 2184, 2394
BspMAI	CTGCAG	2 cut(s)	1228, 1483
BspMI	ACCTGC	5 cut(s)	538, 907, 1217, 1231, 1486
BspOI	GCTAGC	1 cut(s)	2342
BspPI	GGATC	4 cut(s)	330, 1060, 1888, 2595
BspQI	GCTCTTC	1 cut(s)	902
BspTNI	GGTCTC	2 cut(s)	351, 2052
BsrGI	TGTACA	4 cut(s)	1345, 1495, 1655, 2493
BsrI	ACTGG	6 cut(s)	115, 1281, 1761, 1994, 2396, 2637
BssECI	CCNNGG	5 cut(s)	942, 1897, 2171, 2186, 2641
BssMI	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
BssNAI	GTATAC	1 cut(s)	35
BssT1I	CCWWGG	2 cut(s)	942, 2641
Bst1107I	GTATAC	1 cut(s)	35
Bst2UI	CCWGG	1 cut(s)	1050
Bst4CI	ACNGT	6 cut(s)	10, 51, 131, 459, 1967, 2362
Bst6I	CTCTTC	5 cut(s)	708, 891, 902, 1695, 1932
BstAUI	TGTACA	4 cut(s)	1345, 1495, 1655, 2493
BstBAI	YACGTR	1 cut(s)	2612
BstC8I	GCNNGC	5 cut(s)	827, 2247, 2340, 2463, 2626
BstDEI	CTNAG	7 cut(s)	260, 350, 668, 729, 1718, 2063, 2591
BstDSI	CCRYGG	2 cut(s)	942, 1897
BstENI	CCTNNNNNAGG	1 cut(s)	591
BstF5I	GGATG	4 cut(s)	273, 1243, 1498, 1589
BstFNI	CGCG	3 cut(s)	320, 518, 1526
BstHHI	GCGC	2 cut(s)	320, 2624
BstKTI	GATC	9 cut(s)	325, 367, 975, 1055, 1896, 1923, 1981, 2097, 2590
BstMAI	GTCTC	4 cut(s)	351, 668, 1990, 2052
BstMBI	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
BstMCI	CGRYCG	1 cut(s)	2097
BstMWI	GCNNNNNNNGC	5 cut(s)	84, 285, 300, 422, 2165
BstNI	CCWGG	1 cut(s)	1050
BstNSI	RCATGY	4 cut(s)	1352, 2465, 2475, 2628
BstSCI	CCNGG	5 cut(s)	398, 1048, 1935, 2170, 2233
BstSFI	CTRYAG	2 cut(s)	1224, 1479
BstSLI	GKGCMC	2 cut(s)	1215, 1512
BstUI	CGCG	3 cut(s)	320, 518, 1526
BstV1I	GCAGC	6 cut(s)	490, 597, 1564, 2034, 2261, 2329
BstV2I	GAAGAC	3 cut(s)	1743, 1857, 2289
BstX2I	RGATCY	1 cut(s)	2587
BstYI	RGATCY	1 cut(s)	2587
BstZ17I	GTATAC	1 cut(s)	35
BsuRI	GGCC	8 cut(s)	245, 310, 941, 1153, 1723, 2044, 2238, 2568
BtgI	CCRYGG	2 cut(s)	942, 1897
BtgZI	GCGATG	1 cut(s)	1629
BtsCI	GGATG	4 cut(s)	273, 1243, 1498, 1589
BtsIMutI	CAGTG	4 cut(s)	704, 1754, 1827, 2403
BveI	ACCTGC	5 cut(s)	538, 907, 1217, 1231, 1486
Cac8I	GCNNGC	5 cut(s)	827, 2247, 2340, 2463, 2626
CaiI	CAGNNNCTG	3 cut(s)	706, 1457, 1580
CciI	TCATGA	1 cut(s)	405
CfoI	GCGC	2 cut(s)	320, 2624
Cfr13I	GGNCC	5 cut(s)	308, 2183, 2231, 2236, 2392
CseI	GACGC	1 cut(s)	1532
CspCI	CAANNNNNGTGG	2 cut(s)	2259, 2294
DdeI	CTNAG	7 cut(s)	260, 350, 668, 729, 1718, 2063, 2591
DpnI	GATC	9 cut(s)	324, 366, 974, 1054, 1895, 1922, 1980, 2096, 2589
DpnII	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
DraI	TTTAAA	1 cut(s)	1335
EaeI	YGGCCR	2 cut(s)	243, 2566
Eam1104I	CTCTTC	5 cut(s)	708, 891, 902, 1695, 1932
EarI	CTCTTC	5 cut(s)	708, 891, 902, 1695, 1932
Ecl136II	GAGCTC	2 cut(s)	799, 1669
Eco130I	CCWWGG	2 cut(s)	942, 2641
Eco147I	AGGCCT	1 cut(s)	1153
Eco24I	GRGCYC	2 cut(s)	801, 1671
Eco31I	GGTCTC	2 cut(s)	351, 2052
Eco32I	GATATC	1 cut(s)	1842
Eco47I	GGWCC	3 cut(s)	2183, 2231, 2392
Eco53kI	GAGCTC	2 cut(s)	799, 1669
Eco57I	CTGAAG	2 cut(s)	837, 1623
Eco88I	CYCGRG	2 cut(s)	2122, 2583
EcoICRI	GAGCTC	2 cut(s)	799, 1669
EcoNI	CCTNNNNNAGG	1 cut(s)	591
EcoO109I	RGGNCCY	1 cut(s)	308
EcoRI	GAATTC	1 cut(s)	2003
EcoRII	CCWGG	1 cut(s)	1048
EcoRV	GATATC	1 cut(s)	1842
EcoT14I	CCWWGG	2 cut(s)	942, 2641
EcoT22I	ATGCAT	1 cut(s)	2630
EcoT38I	GRGCYC	2 cut(s)	801, 1671
ErhI	CCWWGG	2 cut(s)	942, 2641
Esp3I	CGTCTC	1 cut(s)	668
FalI	AAGNNNNNCTT	2 cut(s)	1711, 1743
FaqI	GGGAC	5 cut(s)	592, 1104, 1372, 1407, 1535
FauI	CCCGC	2 cut(s)	822, 1288
FbaI	TGATCA	1 cut(s)	972
FblI	GTMKAC	2 cut(s)	5, 34
Fnu4HI	GCNGC	8 cut(s)	479, 611, 1578, 2045, 2048, 2250, 2343, 2650
FokI	GGATG	4 cut(s)	260, 1250, 1505, 1576
FriOI	GRGCYC	2 cut(s)	801, 1671
Fsp4HI	GCNGC	8 cut(s)	479, 611, 1578, 2045, 2048, 2250, 2343, 2650
FspBI	CTAG	8 cut(s)	84, 239, 593, 599, 869, 1772, 1946, 2339
GlaI	GCGC	2 cut(s)	319, 2623
GluI	GCNGC	8 cut(s)	479, 611, 1578, 2045, 2048, 2250, 2343, 2650
GsuI	CTGGAG	2 cut(s)	98, 2654
HaeIII	GGCC	8 cut(s)	245, 310, 941, 1153, 1723, 2044, 2238, 2568
HapII	CCGG	7 cut(s)	400, 564, 607, 1937, 2055, 2171, 2234
HgaI	GACGC	1 cut(s)	1532
HhaI	GCGC	2 cut(s)	320, 2624
Hin6I	GCGC	2 cut(s)	318, 2622
HinP1I	GCGC	2 cut(s)	318, 2622
HincII	GTYRAC	3 cut(s)	6, 561, 835
HindII	GTYRAC	3 cut(s)	6, 561, 835
HindIII	AAGCTT	3 cut(s)	1732, 1783, 2402
HpaII	CCGG	7 cut(s)	400, 564, 607, 1937, 2055, 2171, 2234
HphI	GGTGA	7 cut(s)	1159, 1212, 1467, 1543, 1705, 1979, 2044
Hpy188III	TCNNGA	9 cut(s)	406, 869, 976, 1408, 1436, 1982, 2195, 2374, 2585
Hpy99I	CGWCG	1 cut(s)	2095
HpyAV	CCTTC	9 cut(s)	197, 980, 1227, 1307, 1348, 1412, 1522, 1596, 2017
HpyCH4III	ACNGT	6 cut(s)	10, 51, 131, 459, 1967, 2362
HpyCH4IV	ACGT	4 cut(s)	171, 661, 2072, 2611
HpyF10VI	GCNNNNNNNGC	5 cut(s)	84, 285, 300, 422, 2165
HpyF3I	CTNAG	7 cut(s)	260, 350, 668, 729, 1718, 2063, 2591
HpySE526I	ACGT	4 cut(s)	171, 661, 2072, 2611
HspAI	GCGC	2 cut(s)	318, 2622
Ksp22I	TGATCA	1 cut(s)	972
Kzo9I	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
LguI	GCTCTTC	1 cut(s)	902
LmnI	GCTCC	6 cut(s)	256, 589, 751, 1921, 2173, 2321
Lsp1109I	GCAGC	6 cut(s)	490, 597, 1564, 2034, 2261, 2329
LweI	GCATC	8 cut(s)	288, 520, 563, 610, 751, 1159, 1552, 2200
MaeI	CTAG	8 cut(s)	84, 239, 593, 599, 869, 1772, 1946, 2339
MaeII	ACGT	4 cut(s)	171, 661, 2072, 2611
MaeIII	GTNAC	5 cut(s)	394, 625, 1531, 1967, 2068
MalI	GATC	9 cut(s)	324, 366, 974, 1054, 1895, 1922, 1980, 2096, 2589
MboI	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
MfeI	CAATTG	2 cut(s)	289, 1062
MflI	RGATCY	1 cut(s)	2587
MhlI	GDGCHC	4 cut(s)	801, 1215, 1512, 1671
MlsI	TGGCCA	2 cut(s)	245, 2568
MluNI	TGGCCA	2 cut(s)	245, 2568
MlyI	GAGTC	4 cut(s)	956, 1118, 1389, 1413
Mox20I	TGGCCA	2 cut(s)	245, 2568
Mph1103I	ATGCAT	1 cut(s)	2630
MroXI	GAANNNNTTC	2 cut(s)	207, 2352
MscI	TGGCCA	2 cut(s)	245, 2568
MslI	CAYNNNNRTG	1 cut(s)	1118
Msp20I	TGGCCA	2 cut(s)	245, 2568
MspA1I	CMGCKG	2 cut(s)	1577, 2047
MspI	CCGG	7 cut(s)	400, 564, 607, 1937, 2055, 2171, 2234
MspR9I	CCNGG	5 cut(s)	400, 1050, 1937, 2172, 2235
MunI	CAATTG	2 cut(s)	289, 1062
Mva1269I	GAATGC	1 cut(s)	1631
MvaI	CCWGG	1 cut(s)	1050
MvnI	CGCG	3 cut(s)	320, 518, 1526
MwoI	GCNNNNNNNGC	5 cut(s)	84, 285, 300, 422, 2165
NciI	CCSGG	4 cut(s)	400, 1937, 2172, 2235
NcoI	CCATGG	1 cut(s)	942
NdeII	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
NheI	GCTAGC	1 cut(s)	2338
NlaIV	GGNNCC	4 cut(s)	1917, 2169, 2184, 2394
NmuCI	GTSAC	3 cut(s)	394, 1531, 1967
NsiI	ATGCAT	1 cut(s)	2630
NspI	RCATGY	4 cut(s)	1352, 2465, 2475, 2628
OliI	CACNNNNGTG	1 cut(s)	1118
PaeI	GCATGC	2 cut(s)	2465, 2628
PaeR7I	CTCGAG	1 cut(s)	2122
PagI	TCATGA	1 cut(s)	405
PaqCI	CACCTGC	4 cut(s)	907, 1217, 1231, 1486
PceI	AGGCCT	1 cut(s)	1153
PciI	ACATGT	1 cut(s)	1348
PciSI	GCTCTTC	1 cut(s)	902
PcsI	WCGNNNNNNNCGW	1 cut(s)	2120
PctI	GAATGC	1 cut(s)	1631
PdmI	GAANNNNTTC	2 cut(s)	207, 2352
PfeI	GAWTC	7 cut(s)	181, 675, 1271, 1622, 1761, 2131, 2191
PflMI	CCANNNNNTGG	1 cut(s)	1912
PkrI	GCNGC	8 cut(s)	480, 612, 1579, 2046, 2049, 2251, 2344, 2651
Ple19I	CGATCG	1 cut(s)	2097
PleI	GAGTC	4 cut(s)	955, 1118, 1389, 1412
PpsI	GAGTC	4 cut(s)	955, 1118, 1389, 1412
Ppu21I	YACGTR	1 cut(s)	2612
PscI	ACATGT	1 cut(s)	1348
PshBI	ATTAAT	1 cut(s)	1377
Psp124BI	GAGCTC	2 cut(s)	801, 1671
Psp1406I	AACGTT	1 cut(s)	171
Psp6I	CCWGG	1 cut(s)	1048
PspGI	CCWGG	1 cut(s)	1048
PspN4I	GGNNCC	4 cut(s)	1917, 2169, 2184, 2394
PspPI	GGNCC	5 cut(s)	308, 2183, 2231, 2236, 2392
PspXI	VCTCGAGB	1 cut(s)	2122
PsrI	GAACNNNNNNTAC	2 cut(s)	2477, 2509
PstI	CTGCAG	2 cut(s)	1228, 1483
PstNI	CAGNNNCTG	3 cut(s)	706, 1457, 1580
PsuI	RGATCY	1 cut(s)	2587
PvuI	CGATCG	1 cut(s)	2097
PvuII	CAGCTG	1 cut(s)	1577
RseI	CAYNNNNRTG	1 cut(s)	1118
SacI	GAGCTC	2 cut(s)	801, 1671
SalI	GTCGAC	1 cut(s)	4
SapI	GCTCTTC	1 cut(s)	902
SatI	GCNGC	8 cut(s)	479, 611, 1578, 2045, 2048, 2250, 2343, 2650
Sau3AI	GATC	9 cut(s)	322, 364, 972, 1052, 1893, 1920, 1978, 2094, 2587
Sau96I	GGNCC	5 cut(s)	308, 2183, 2231, 2236, 2392
SbfI	CCTGCAGG	1 cut(s)	1228
ScaI	AGTACT	1 cut(s)	237
SchI	GAGTC	4 cut(s)	956, 1118, 1389, 1413
ScrFI	CCNGG	5 cut(s)	400, 1050, 1937, 2172, 2235
SdaI	CCTGCAGG	1 cut(s)	1228
SduI	GDGCHC	4 cut(s)	801, 1215, 1512, 1671
SfaNI	GCATC	8 cut(s)	288, 520, 563, 610, 751, 1159, 1552, 2200
SfcI	CTRYAG	2 cut(s)	1224, 1479
Sfr274I	CTCGAG	1 cut(s)	2122
SinI	GGWCC	3 cut(s)	2183, 2231, 2392
SlaI	CTCGAG	1 cut(s)	2122
SmiMI	CAYNNNNRTG	1 cut(s)	1118
SmlI	CTYRAG	1 cut(s)	2122
SmoI	CTYRAG	1 cut(s)	2122
SpeI	ACTAGT	2 cut(s)	238, 598
SphI	GCATGC	2 cut(s)	2465, 2628
Sse8387I	CCTGCAGG	1 cut(s)	1228
SseBI	AGGCCT	1 cut(s)	1153
SsiI	CCGC	8 cut(s)	277, 320, 829, 1295, 2045, 2296, 2345, 2649
SspMI	CTAG	8 cut(s)	84, 239, 593, 599, 869, 1772, 1946, 2339
SstI	GAGCTC	2 cut(s)	801, 1671
StuI	AGGCCT	1 cut(s)	1153
StyD4I	CCNGG	5 cut(s)	398, 1048, 1935, 2170, 2233
StyI	CCWWGG	2 cut(s)	942, 2641
TaaI	ACNGT	6 cut(s)	10, 51, 131, 459, 1967, 2362
TaiI	ACGT	4 cut(s)	174, 664, 2075, 2614
TaqI	TCGA	7 cut(s)	5, 683, 1290, 1981, 2007, 2090, 2123
TatI	WGTACW	7 cut(s)	235, 846, 1240, 1345, 1495, 1655, 2493
TauI	GCSGC	2 cut(s)	2047, 2652
TfiI	GAWTC	7 cut(s)	181, 675, 1271, 1622, 1761, 2131, 2191
TscAI	CASTG	4 cut(s)	711, 1761, 1834, 2403
TseFI	GTSAC	3 cut(s)	394, 1531, 1967
TseI	GCWGC	6 cut(s)	478, 610, 1577, 2047, 2249, 2342
Tsp45I	GTSAC	3 cut(s)	394, 1531, 1967
TspDTI	ATGAA	8 cut(s)	236, 348, 1118, 1518, 1578, 1632, 2345, 2548
TspGWI	ACGGA	4 cut(s)	157, 1527, 1886, 2129
TspRI	CASTG	4 cut(s)	711, 1761, 1834, 2403
Van91I	CCANNNNNTGG	1 cut(s)	1912
VneI	GTGCAC	1 cut(s)	1508
VpaK11BI	GGWCC	3 cut(s)	2183, 2231, 2392
VspI	ATTAAT	1 cut(s)	1377
XagI	CCTNNNNNAGG	1 cut(s)	591
XapI	RAATTY	6 cut(s)	864, 1367, 1542, 2003, 2108, 2144
XbaI	TCTAGA	1 cut(s)	868
XceI	RCATGY	4 cut(s)	1352, 2465, 2475, 2628
XhoI	CTCGAG	1 cut(s)	2122
XmiI	GTMKAC	2 cut(s)	5, 34
XmnI	GAANNNNTTC	2 cut(s)	207, 2352
XspI	CTAG	8 cut(s)	84, 239, 593, 599, 869, 1772, 1946, 2339
ZrmI	AGTACT	1 cut(s)	237
Zsp2I	ATGCAT	1 cut(s)	2630

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.