RLG00000004827
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000408
Physical Location & Seq
Reverse (-)
62849205 .. 62850161
957 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004827

Sequence Viewer

Length: 957 bp
ATGCCGGCCAAGCTGATCGGAGGACCGCTGTATAATCAGCTCTTTGATGGTGTTAAGACAGCAACAGGCAGAAGTACAAAGTTCACCTCCCTCCTCGAAGATCTCAAATTCACGCTAGTCTCTCTGCAACCACGAATCAATCGACAGAGAGGAGATCAGCACGATGCGGAAATTCATCATCTCCTTCCAAATGAGGAAATACAAGATCTTGAGCGACAGATGGAGGAGGGAGTGAAGCTGGTTGCCAAGCTATCAAGTGTTGGAATATGGAACTACCACTGCATGAAGAGTAACTACTCCGACCAACTAGCTCAATTGGATAGGTCTCTCAGAAAAATGTTATACATACTCAAGCTGCAGGAAGAAAGAGATATAAAGGAGCTCTTGCTTGTTGCTAGGACAATACGAGACAGACAGAATGTTTTGCAAAGAAGACTATGTGGTTCACTCAAAGTGCAGCAGGAGCAAACCGATGGCAATAGTCACGTCGACAACGAAGGAGGAGGGGCTGGTCGGGATCTGGAGCATGTATTTTGCCGTCTACTTGAATCCCTTGTTTTGGAAGTTTATGTACAATTGAATGGGAAAACGAGGTTCAGTCCCGTCTTGGCGGATCTGAAATCGACGGTTAGGTCCTTCCAACCACTCATGGAAGAAATATCAGATTATAACAAGGTATTGCATCTACCAAAGGATGAACTAGACAATTTGAGGAAGCAACTGGAGAAAGGTATCGAGCTGGTCGGCAAGTGCTCCAAGGTTCCTCAAGGGGCAAGCTACAAAAAGTATGAATACGCCGACGAACTACTTGAGTTGGATGACTATCTTCAGGGCTTGTTGTGTTTGATGAGGGAGCAGGTGGCAAAGGATTTGAAGGAGACCTTGGTTTCTGTAAGCAACATGGAGATGATGTTAAAGCGAATTGAAGAGGGTAGTAGTGGTCTGGCACAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

319

Amino Acids

36.37

Weight (kDa)

6.05

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 12 - 131 6.1e-16 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 185 - 306 4.8e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AarI CACCTGC 1 cut(s) 847
AasI GACNNNNNNGTC 1 cut(s) 631
Acc36I ACCTGC 1 cut(s) 847
AccI GTMKAC 2 cut(s) 489, 541
AciI CCGC 3 cut(s) 26, 167, 611
AclWI GGATC 2 cut(s) 525, 621
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 2 cut(s) 107, 171
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 2 cut(s) 76, 573
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 4 cut(s) 548, 580, 874, 926
AjiI CACGTC 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
AluBI AGCT 9 cut(s) 13, 40, 238, 250, 311, 355, 382, 739, 777
AluI AGCT 9 cut(s) 13, 40, 238, 250, 311, 355, 382, 739, 777
Alw21I GWGCWC 2 cut(s) 384, 755
Alw26I GTCTC 4 cut(s) 124, 330, 402, 872
AlwI GGATC 2 cut(s) 525, 621
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 355, 457
ApoI RAATTY 2 cut(s) 107, 171
AspS9I GGNCC 2 cut(s) 23, 633
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 2 cut(s) 23, 633
BanII GRGCYC 1 cut(s) 384
BarI GAAGNNNNNNTAC 4 cut(s) 278, 310, 555, 587
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 2 cut(s) 384, 755
BbvI GCAGC 2 cut(s) 342, 469
BccI CCATC 3 cut(s) 41, 214, 467
BceAI ACGGC 1 cut(s) 522
BcoDI GTCTC 4 cut(s) 124, 330, 402, 872
BfaI CTAG 4 cut(s) 116, 308, 396, 701
BfmI CTRYAG 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 847
BglII AGATCT 2 cut(s) 100, 205
BisI GCNGC 2 cut(s) 356, 458
BlsI GCNGC 2 cut(s) 357, 459
Bme18I GGWCC 2 cut(s) 23, 633
BmgBI CACGTC 1 cut(s) 487
BmgT120I GGNCC 2 cut(s) 23, 633
BmiI GGNNCC 1 cut(s) 762
BmsI GCATC 2 cut(s) 154, 691
BpiI GAAGAC 1 cut(s) 439
BpmI CTGGAG 2 cut(s) 542, 743
BpuEI CTTGAG 4 cut(s) 230, 335, 750, 830
BsaBI GATNNNNATC 1 cut(s) 822
BsaI GGTCTC 2 cut(s) 330, 872
BsaJI CCNNGG 2 cut(s) 756, 882
BsaXI ACNNNNNCTCC 2 cut(s) 896, 926
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 726
Bse8I GATNNNNATC 1 cut(s) 822
BseDI CCNNGG 2 cut(s) 756, 882
BseGI GGATG 2 cut(s) 700, 823
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMII CTCAG 1 cut(s) 343
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 4 cut(s) 83, 165, 239, 516
BseXI GCAGC 2 cut(s) 342, 469
BsgI GTGCAG 1 cut(s) 476
BshFI GGCC 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 384, 755
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 4 cut(s) 124, 330, 402, 872
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 2 cut(s) 330, 872
Bsp1286I GDGCHC 2 cut(s) 384, 755
Bsp1407I TGTACA 1 cut(s) 571
Bsp143I GATC 6 cut(s) 15, 100, 154, 205, 517, 613
BspACI CCGC 3 cut(s) 26, 167, 611
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 762
BspMAI CTGCAG 1 cut(s) 360
BspMI ACCTGC 1 cut(s) 847
BspPI GGATC 2 cut(s) 525, 621
BspTNI GGTCTC 2 cut(s) 330, 872
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 571
BsrI ACTGG 1 cut(s) 726
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 756, 882
BssMI GATC 6 cut(s) 15, 100, 154, 205, 517, 613
BssT1I CCWWGG 2 cut(s) 756, 882
Bst4CI ACNGT 1 cut(s) 628
Bst6I CTCTTC 2 cut(s) 281, 921
BstAUI TGTACA 1 cut(s) 571
BstC8I GCNNGC 2 cut(s) 6, 775
BstDEI CTNAG 1 cut(s) 329
BstF5I GGATG 2 cut(s) 700, 823
BstKTI GATC 6 cut(s) 18, 103, 157, 208, 520, 616
BstMAI GTCTC 4 cut(s) 124, 330, 402, 872
BstMBI GATC 6 cut(s) 15, 100, 154, 205, 517, 613
BstMWI GCNNNNNNNGC 2 cut(s) 10, 463
BstNSI RCATGY 1 cut(s) 530
BstSFI CTRYAG 1 cut(s) 356
BstV1I GCAGC 2 cut(s) 342, 469
BstV2I GAAGAC 1 cut(s) 439
BstX2I RGATCY 4 cut(s) 100, 205, 517, 613
BstYI RGATCY 4 cut(s) 100, 205, 517, 613
BsuRI GGCC 1 cut(s) 8
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 2 cut(s) 700, 823
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 277
BveI ACCTGC 1 cut(s) 847
Cac8I GCNNGC 2 cut(s) 6, 775
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 2 cut(s) 23, 633
Csp6I GTAC 2 cut(s) 75, 572
CviAII CATG 4 cut(s) 283, 527, 649, 901
CviQI GTAC 2 cut(s) 75, 572
DdeI CTNAG 1 cut(s) 329
DpnI GATC 6 cut(s) 17, 102, 156, 207, 519, 615
DpnII GATC 6 cut(s) 15, 100, 154, 205, 517, 613
DrdI GACNNNNNNGTC 1 cut(s) 631
DseDI GACNNNNNNGTC 1 cut(s) 631
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 281, 921
EarI CTCTTC 2 cut(s) 281, 921
EciI GGCGGA 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 2 cut(s) 756, 882
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 2 cut(s) 330, 872
Eco47I GGWCC 2 cut(s) 23, 633
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 812
EcoICRI GAGCTC 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 633
EcoT14I CCWWGG 2 cut(s) 756, 882
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 2 cut(s) 756, 882
FaeI CATG 4 cut(s) 286, 530, 652, 904
FalI AAGNNNNNCTT 4 cut(s) 368, 400, 866, 898
FaqI GGGAC 1 cut(s) 585
FatI CATG 4 cut(s) 282, 526, 648, 900
FblI GTMKAC 2 cut(s) 489, 541
Fnu4HI GCNGC 2 cut(s) 356, 458
FokI GGATG 2 cut(s) 707, 830
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 2 cut(s) 356, 458
FspBI CTAG 4 cut(s) 116, 308, 396, 701
GluI GCNGC 2 cut(s) 356, 458
GsuI CTGGAG 2 cut(s) 542, 743
HaeIII GGCC 1 cut(s) 8
HapII CCGG 1 cut(s) 5
Hin1II CATG 4 cut(s) 286, 530, 652, 904
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 2 cut(s) 135, 548
HpaII CCGG 1 cut(s) 5
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 4 cut(s) 84, 446, 490, 542
Hpy188I TCNGA 5 cut(s) 20, 301, 332, 618, 664
Hpy188III TCNNGA 3 cut(s) 209, 515, 521
Hpy8I GTNNAC 4 cut(s) 84, 446, 490, 542
Hpy99I CGWCG 3 cut(s) 491, 628, 803
HpyAV CCTTC 4 cut(s) 194, 491, 646, 868
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 6 cut(s) 127, 282, 358, 427, 457, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 463
HpyF3I CTNAG 1 cut(s) 329
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 4 cut(s) 286, 530, 652, 904
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 6 cut(s) 15, 100, 154, 205, 517, 613
LmnI GCTCC 5 cut(s) 379, 463, 523, 758, 853
Lsp1109I GCAGC 2 cut(s) 342, 469
LweI GCATC 2 cut(s) 154, 691
MaeI CTAG 4 cut(s) 116, 308, 396, 701
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 290, 482
MalI GATC 6 cut(s) 17, 102, 156, 207, 519, 615
MboI GATC 6 cut(s) 15, 100, 154, 205, 517, 613
MboII GAAGA 7 cut(s) 110, 298, 374, 444, 665, 818, 938
MfeI CAATTG 2 cut(s) 314, 575
MflI RGATCY 4 cut(s) 100, 205, 517, 613
MhlI GDGCHC 2 cut(s) 384, 755
MluCI AATT 7 cut(s) 107, 171, 314, 575, 706, 921, 952
MmeI TCCRAC 4 cut(s) 241, 324, 664, 795
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 3 cut(s) 54, 914, 955
MslI CAYNNNNRTG 1 cut(s) 905
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 1 cut(s) 5
MunI CAATTG 2 cut(s) 314, 575
MwoI GCNNNNNNNGC 2 cut(s) 10, 463
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 6 cut(s) 15, 100, 154, 205, 517, 613
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 4 cut(s) 286, 530, 652, 904
NlaIV GGNNCC 1 cut(s) 762
NmuCI GTSAC 1 cut(s) 482
NspI RCATGY 1 cut(s) 530
PaqCI CACCTGC 1 cut(s) 847
PcsI WCGNNNNNNNCGW 2 cut(s) 139, 492
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 2 cut(s) 135, 548
PkrI GCNGC 2 cut(s) 357, 459
PpuMI RGGWCCY 1 cut(s) 633
PsiI TTATAA 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 633
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 2 cut(s) 23, 633
PspPPI RGGWCCY 1 cut(s) 633
PstI CTGCAG 1 cut(s) 360
PsuI RGATCY 4 cut(s) 100, 205, 517, 613
RsaI GTAC 2 cut(s) 76, 573
RsaNI GTAC 2 cut(s) 75, 572
RseI CAYNNNNRTG 1 cut(s) 905
SacI GAGCTC 1 cut(s) 384
SalI GTCGAC 1 cut(s) 488
SaqAI TTAA 3 cut(s) 54, 914, 955
SatI GCNGC 2 cut(s) 356, 458
Sau3AI GATC 6 cut(s) 15, 100, 154, 205, 517, 613
Sau96I GGNCC 2 cut(s) 23, 633
SduI GDGCHC 2 cut(s) 384, 755
SfaNI GCATC 2 cut(s) 154, 691
SfcI CTRYAG 1 cut(s) 356
SinI GGWCC 2 cut(s) 23, 633
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 4 cut(s) 209, 350, 765, 809
SmoI CTYRAG 4 cut(s) 209, 350, 765, 809
Sse9I AATT 7 cut(s) 107, 171, 314, 575, 706, 921, 952
SsiI CCGC 3 cut(s) 26, 167, 611
SspMI CTAG 4 cut(s) 116, 308, 396, 701
SstI GAGCTC 1 cut(s) 384
StyI CCWWGG 2 cut(s) 756, 882
TaaI ACNGT 1 cut(s) 628
TaiI ACGT 1 cut(s) 489
TaqI TCGA 5 cut(s) 96, 142, 489, 623, 735
TasI AATT 7 cut(s) 107, 171, 314, 575, 706, 921, 952
TatI WGTACW 2 cut(s) 74, 571
TfiI GAWTC 2 cut(s) 135, 548
Tru1I TTAA 3 cut(s) 54, 914, 955
Tru9I TTAA 3 cut(s) 54, 914, 955
TscAI CASTG 1 cut(s) 284
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 2 cut(s) 355, 457
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 4 cut(s) 164, 299, 711, 804
TspRI CASTG 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 23, 633
XapI RAATTY 2 cut(s) 107, 171
XceI RCATGY 1 cut(s) 530
XmiI GTMKAC 2 cut(s) 489, 541
XspI CTAG 4 cut(s) 116, 308, 396, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.