Rh7CG338000

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000408
Physical Location & Seq
Forward (+)
37757472 .. 37758197
726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG338000.1

Sequence Viewer

Length: 726 bp
ATGGCTCTGGAATTGATCGGAGGAGCAGTATTTGCAGTGCTGTATGATGGCGTTAAGCAAGCTATGGGTAGGACTGAGACGTTCAAAGAGCTCCTCAGAGATCTCAAGTTCACCTTGGAGTGTTTAAGACGGCAGGCCACGGCCATCCAACAGATAGGCCAGTATAACGTGGAATTAGGTCTCCCAAACGATGAGATAGAGGAACTTGAAAGACAAATGCACAGCGGAACACGACTCGTGGGGAAGTCGTTGAATTTTCGGAAGTGGAACTACTGCTGCATAAATTGTTACACAGACCAACTAGTCGACTTGGATAGGTCCCTAAGAAGACTGTTGGAAAACCTCATGTTGCAGGAGATAAGGGACGTGAAGGAGATATTGGATCTGTCGAGGAAAAGCCGCAAGGAGCTTGAAGAAGTGAAGGAGGCACAGTTGAGTATGCACAAGTTAATGCAGCAGATAGCAGGAGTAGTTGTAATGGAAGAGGCCTCAACTTCGGCTGCTGCAGGATTAGGTGCTATTGAGATGGTGCCGAATCAAAATGAAGGGAATGGCGGCATTCAAGGTGAAATCATTGGAAATTGCTTTGTCAAATTTTATATTGGCCTTCCGGCTATAGAAGTTATTACACTACTTCGCTTAGTACATTTGGTATTTGTGCTTAAAGTTCTATTCATATTCGATGTTATTAGCTTCCGAAAGCCTAATGTAATAGTGCCTTTCTAA

Protein Analysis

241

Amino Acids

27.32

Weight (kDa)

5.76

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 137 9.7e-16 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 529
AccI GTMKAC 1 cut(s) 306
AciI CCGC 3 cut(s) 225, 400, 555
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 2 cut(s) 253, 593
AfaI GTAC 1 cut(s) 645
AgsI TTSAA 5 cut(s) 85, 209, 253, 413, 563
AhlI ACTAGT 1 cut(s) 301
AjiI CACGTC 1 cut(s) 367
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 62, 91, 409, 693
AluI AGCT 4 cut(s) 62, 91, 409, 693
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 2 cut(s) 71, 185
AlwI GGATC 1 cut(s) 390
AoxI GGCC 5 cut(s) 135, 141, 157, 486, 604
ApeKI GCWGC 4 cut(s) 276, 454, 500, 503
ApoI RAATTY 2 cut(s) 253, 593
AspS9I GGNCC 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 103, 578
AvaII GGWCC 1 cut(s) 318
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 93
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 4 cut(s) 263, 466, 487, 490
BccI CCATC 3 cut(s) 41, 152, 520
BceAI ACGGC 2 cut(s) 146, 156
BcoDI GTCTC 2 cut(s) 71, 185
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 2 cut(s) 504, 615
BglII AGATCT 1 cut(s) 100
BisI GCNGC 6 cut(s) 277, 400, 455, 501, 504, 556
BlsI GCNGC 6 cut(s) 278, 401, 456, 502, 505, 557
Bme18I GGWCC 1 cut(s) 318
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 2 cut(s) 320, 531
BpiI GAAGAC 1 cut(s) 334
BpuEI CTTGAG 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 2 cut(s) 114, 138
BsaXI ACNNNNNCTCC 6 cut(s) 12, 42, 416, 446, 459, 489
Bse1I ACTGG 1 cut(s) 160
BseDI CCNNGG 2 cut(s) 114, 138
BseGI GGATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 66, 109
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 36, 83
BseXI GCAGC 4 cut(s) 263, 466, 487, 490
BshFI GGCC 5 cut(s) 137, 143, 159, 488, 606
BshNI GGYRCC 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 93
BsiSI CCGG 1 cut(s) 611
BslFI GGGAC 2 cut(s) 304, 377
BsmAI GTCTC 2 cut(s) 71, 185
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 2 cut(s) 304, 377
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 5 cut(s) 137, 143, 159, 488, 606
Bso31I GGTCTC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 3 cut(s) 15, 100, 382
BspACI CCGC 3 cut(s) 225, 400, 555
BspANI GGCC 5 cut(s) 137, 143, 159, 488, 606
BspCNI CTCAG 2 cut(s) 67, 108
BspLI GGNNCC 2 cut(s) 320, 531
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 529
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 114, 138
BssMI GATC 3 cut(s) 15, 100, 382
BssSI CACGAG 1 cut(s) 236
BssT1I CCWWGG 1 cut(s) 114
Bst2BI CACGAG 1 cut(s) 236
Bst4CI ACNGT 2 cut(s) 333, 432
Bst6I CTCTTC 1 cut(s) 477
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 60, 135
BstDEI CTNAG 4 cut(s) 75, 95, 323, 640
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 144
BstKTI GATC 3 cut(s) 18, 103, 385
BstMAI GTCTC 2 cut(s) 71, 185
BstMBI GATC 3 cut(s) 15, 100, 382
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstSFI CTRYAG 2 cut(s) 504, 615
BstV1I GCAGC 4 cut(s) 263, 466, 487, 490
BstV2I GAAGAC 1 cut(s) 334
BstX2I RGATCY 2 cut(s) 100, 382
BstYI RGATCY 2 cut(s) 100, 382
BsuRI GGCC 5 cut(s) 137, 143, 159, 488, 606
BtgI CCRYGG 1 cut(s) 138
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 144
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 2 cut(s) 60, 135
Cfr13I GGNCC 1 cut(s) 318
Csp6I GTAC 1 cut(s) 644
CviAII CATG 1 cut(s) 346
CviQI GTAC 1 cut(s) 644
DdeI CTNAG 4 cut(s) 75, 95, 323, 640
DpnI GATC 3 cut(s) 17, 102, 384
DpnII GATC 3 cut(s) 15, 100, 382
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 318
Eco53kI GAGCTC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 1 cut(s) 349
FaiI YATR 9 cut(s) 45, 65, 165, 281, 347, 440, 600, 617, 677
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 2 cut(s) 304, 377
FatI CATG 1 cut(s) 345
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 6 cut(s) 277, 400, 455, 501, 504, 556
FokI GGATG 1 cut(s) 131
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 6 cut(s) 277, 400, 455, 501, 504, 556
FspBI CTAG 1 cut(s) 302
GluI GCNGC 6 cut(s) 277, 400, 455, 501, 504, 556
HaeIII GGCC 5 cut(s) 137, 143, 159, 488, 606
HapII CCGG 1 cut(s) 611
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 2 cut(s) 234, 535
HpaII CCGG 1 cut(s) 611
HphI GGTGA 2 cut(s) 103, 578
Hpy166II GTNNAC 2 cut(s) 111, 307
Hpy188I TCNGA 4 cut(s) 20, 98, 261, 698
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 2 cut(s) 111, 307
HpyAV CCTTC 4 cut(s) 364, 415, 539, 617
HpyCH4III ACNGT 2 cut(s) 333, 432
HpyCH4IV ACGT 3 cut(s) 80, 168, 366
HpyCH4V TGCA 7 cut(s) 35, 220, 279, 352, 442, 454, 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 4 cut(s) 75, 95, 323, 640
HpySE526I ACGT 3 cut(s) 80, 168, 366
Hsp92II CATG 1 cut(s) 349
Kzo9I GATC 3 cut(s) 15, 100, 382
LmnI GCTCC 3 cut(s) 23, 96, 406
LpnPI CCDG 6 cut(s) 119, 173, 338, 450, 492, 624
Lsp1109I GCAGC 4 cut(s) 263, 466, 487, 490
MaeI CTAG 1 cut(s) 302
MaeII ACGT 3 cut(s) 80, 168, 366
MaeIII GTNAC 1 cut(s) 287
MalI GATC 3 cut(s) 17, 102, 384
MboI GATC 3 cut(s) 15, 100, 382
MboII GAAGA 3 cut(s) 339, 425, 494
MflI RGATCY 2 cut(s) 100, 382
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 6 cut(s) 11, 173, 253, 283, 580, 593
MlyI GAGTC 1 cut(s) 228
MmeI TCCRAC 2 cut(s) 172, 315
MnlI CCTC 8 cut(s) 14, 104, 193, 353, 384, 418, 478, 499
MseI TTAA 4 cut(s) 54, 125, 449, 663
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 1 cut(s) 611
Mva1269I GAATGC 1 cut(s) 558
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeII GATC 3 cut(s) 15, 100, 382
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 2 cut(s) 320, 531
PceI AGGCCT 1 cut(s) 488
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 1 cut(s) 535
PkrI GCNGC 6 cut(s) 278, 401, 456, 502, 505, 557
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 320, 531
PspPI GGNCC 1 cut(s) 318
PspPPI RGGWCCY 1 cut(s) 318
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 2 cut(s) 100, 382
RsaI GTAC 1 cut(s) 645
RsaNI GTAC 1 cut(s) 644
SacI GAGCTC 1 cut(s) 93
SalI GTCGAC 1 cut(s) 305
SaqAI TTAA 4 cut(s) 54, 125, 449, 663
SatI GCNGC 6 cut(s) 277, 400, 455, 501, 504, 556
Sau3AI GATC 3 cut(s) 15, 100, 382
Sau96I GGNCC 1 cut(s) 318
SchI GAGTC 1 cut(s) 228
SduI GDGCHC 1 cut(s) 93
SfcI CTRYAG 2 cut(s) 504, 615
SinI GGWCC 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 301
Sse9I AATT 6 cut(s) 11, 173, 253, 283, 580, 593
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 3 cut(s) 225, 400, 555
SspMI CTAG 1 cut(s) 302
SstI GAGCTC 1 cut(s) 93
StuI AGGCCT 1 cut(s) 488
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 2 cut(s) 333, 432
TaiI ACGT 3 cut(s) 83, 171, 369
TaqI TCGA 3 cut(s) 306, 389, 681
TasI AATT 6 cut(s) 11, 173, 253, 283, 580, 593
TatI WGTACW 1 cut(s) 643
TauI GCSGC 2 cut(s) 402, 558
TfiI GAWTC 1 cut(s) 535
Tru1I TTAA 4 cut(s) 54, 125, 449, 663
Tru9I TTAA 4 cut(s) 54, 125, 449, 663
TscAI CASTG 1 cut(s) 42
TseI GCWGC 4 cut(s) 276, 454, 500, 503
TspDTI ATGAA 2 cut(s) 558, 664
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 2 cut(s) 253, 593
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.