Rh7BG081200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000408
Physical Location & Seq
Reverse (-)
5864029 .. 5865942
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG081200.1

Sequence Viewer

Length: 1914 bp
ATGTCTATGAGTCTGAACTTTCTGGGAGGAGCACCTTTTCAACTCCTATACGATGGTGTTAGGGCAGCCATAGGTAGAACTACCAAGTTCAAAGCCCTCCTCTTAAATCTCGAATCGACGCTAGACTGTTTGCAACCACGAGTCATCCAGCAGATACGAGACCATAATGTGGTGCTGAATCTCCCAAACGATATAATTCAAGACCTGCAAGTAAAAATGGATGATGGTGTGGCACTCATTGCCAATTTATCTAGGCTTAGCGTGTGGAACTGTCGCATATGGGGGGACTGTTGCAACTGCATGAAGCCTAGCTATGCAGACCAACTTGCTGCATTGGATAGGTCTCTTAGACGACTGTTGGAGATACTAAAGGTCGAGCAGATGAGGAACGTGGTGGAGCTCTTGCTTTTGGCAAGGAGAAGTAATGACCGACAAGATGAATTGGAGAGAAGACAGTTGGAAATTCTGAAAGCGCTGCAAGAAACCGGGGATATGTTAAGAGCGCAGCAGGAAGTAATGGAGAGAATTGAAAGGAATGGTGCTGCATCTTCAGACAGAGGAGGGTCAGCTCCTGCTCCTGCTCTAGGAACAGTATTTAGGGTGTTGTTTGATGTTGTTATCAGAGTAAAGGTCAAGAATATGATGTATGAGCGCCTCCTCAAAGATTTTGAATCCACTCTTGAATGCTTGAAGCCACTTATAGAAGAGATGGTGGAGTCTAACAAGTTGCTGCATCTTCCAAATGAGGAACAAGAATATTTCATAATGCAAATGGAAAAGGGTGTAGAGTTAATTCACCAGTGCTCTGAGGCTTGCAAGAGAGGAATAAGGTATAAAAAGTACGAATACACCAAGAAACTTCTGGGATTGGATGAATGTCTTCAAAGGCTTTTCATTGAACTCAAAGAGCAGGTAGTAAGGAATGTGAAGGAGGCCTTGGTGCTTATAAGTAACTTGGAAAAAGAGATCGCAGAAATTGAAGGGGTTGTTGTGGTACAAAGTGATGAAATTGAGTGTTCGATTCCAGCACCTCAACCTCGATCACCTTTAGTTGGAGAGGATTTACAAAATGTGCAGATAAATCGAGAGGTGATCGAGGAGACATTCGATGCAGTAAGTAACTTAGAAGAGGAGATCAAGGAAATTAGAGAAGTTAATGTGGTACAAAATGATGAAGTACAAAGTAAAGGTCCTCAACCTTCATTGCCTACAGCTGGATCCAGTGTACAAAGTATGCAGGGAACAAGGGATGTGATCAAGGAAAGATGGGACTCGGAAAAGTTGCCGGACGTCAAGCAAATTAAAGGGAAAGGCTTGTTCGAATATCCGGTTGCTGGTGAATATGACCTTCCACCATTTCCAGTGGGACTTGCACTGGAACGAGAAGATCACGCTGATGCATACAGTAATGCATCATTGGAAGAACCAGTTGATGAATTGGATATGCATGTCAGCTACTTGAAAGGGAAGCTTTTATATGGTGGTCACTTAGCCATTGTGCTGACGGGACCTGAAGGATGCGGGAAAACTAAATTGGCAGAAAAGTTTTGTCAAGATGAGGAAGTCATAGATGAATTCAAGAACAATATTTTCTTCGTCCCTGTTTCGGAAAGCCCCAATTTGCAGCTTATTGTTCAAAAACTATATCCAGAGGGCTACAGGCAAATTATGTTGCCAGATGAAAGTGGAGTTCATGCAATTCAGTGGCTGCAAGCATTTGTTAAGCATGTAGGACATCGTCGTTCTCTGTTGGTCCTAGATGATGTCTGGCCTGGATCTGAATCCCTTCTTGATGAGTTTGATGAATTCAAAAGGTCAAATTTCAAGATTCTGGTGACATCAAGATTCGAATTTCCAAGATTTGGTTCCTCCTATTATGTAATGATAGATGATGTACTTAGGGTAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

637

Amino Acids

72.63

Weight (kDa)

5.01

Isoelectric Point (pI)

58.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 137 2.6e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 193 - 323 2.5e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 483 - 615 4.9e-10 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 947
AatII GACGTC 1 cut(s) 1293
Acc36I ACCTGC 2 cut(s) 213, 901
AccB7I CCANNNNNTGG 2 cut(s) 169, 1862
AciI CCGC 1 cut(s) 1521
AclWI GGATC 3 cut(s) 1212, 1225, 1783
AcsI RAATTY 5 cut(s) 462, 1574, 1805, 1819, 1850
AcuI CTGAAG 2 cut(s) 534, 1533
AcyI GRCGYC 1 cut(s) 1290
AfaI GTAC 6 cut(s) 842, 996, 1164, 1179, 1227, 1896
AfeI AGCGCT 1 cut(s) 474
AfiI CCNNNNNNNGG 7 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1862
AjnI CCWGG 1 cut(s) 1771
AjuI GAANNNNNNNTTGG 6 cut(s) 920, 952, 1517, 1549, 1849, 1881
AluBI AGCT 7 cut(s) 312, 400, 569, 1214, 1455, 1471, 1627
AluI AGCT 7 cut(s) 312, 400, 569, 1214, 1455, 1471, 1627
Alw21I GWGCWC 3 cut(s) 34, 402, 806
Alw26I GTCTC 3 cut(s) 153, 348, 1094
AlwI GGATC 3 cut(s) 1212, 1225, 1783
AlwNI CAGNNNCTG 2 cut(s) 572, 1708
Aor51HI AGCGCT 1 cut(s) 474
AoxI GGCC 2 cut(s) 933, 1769
ApeKI GCWGC 8 cut(s) 65, 329, 475, 505, 542, 730, 1624, 1708
ApoI RAATTY 5 cut(s) 462, 1574, 1805, 1819, 1850
ArsI GACNNNNNNTTYG 2 cut(s) 1174, 1206
Asp700I GAANNNNTTC 2 cut(s) 879, 1785
AspLEI GCGC 3 cut(s) 475, 505, 654
AspS9I GGNCC 3 cut(s) 1190, 1508, 1753
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 5 cut(s) 788, 1035, 1102, 1349, 1846
AsuII TTCGAA 2 cut(s) 1320, 1848
AvaII GGWCC 3 cut(s) 1190, 1508, 1753
BamHI GGATCC 1 cut(s) 1217
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 457, 872
Bbv12I GWGCWC 3 cut(s) 34, 402, 806
BbvI GCAGC 8 cut(s) 77, 316, 462, 517, 529, 717, 1636, 1695
BccI CCATC 4 cut(s) 47, 218, 703, 1260
BcgI CGANNNNNNTGC 2 cut(s) 1064, 1098
BciT130I CCWGG 1 cut(s) 1773
BclI TGATCA 1 cut(s) 1254
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 3 cut(s) 153, 348, 1094
BfaI CTAG 5 cut(s) 122, 252, 309, 584, 1757
BfmI CTRYAG 2 cut(s) 1209, 1657
BfoI RGCGCY 2 cut(s) 476, 655
BfuAI ACCTGC 2 cut(s) 213, 901
BisI GCNGC 8 cut(s) 66, 330, 476, 506, 543, 731, 1625, 1709
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 8 cut(s) 67, 331, 477, 507, 544, 732, 1626, 1710
Bme1390I CCNGG 2 cut(s) 487, 1773
Bme18I GGWCC 3 cut(s) 1190, 1508, 1753
BmgT120I GGNCC 3 cut(s) 1190, 1508, 1753
BmiI GGNNCC 3 cut(s) 1219, 1509, 1867
BmrFI CCNGG 2 cut(s) 487, 1773
BmsI GCATC 6 cut(s) 554, 742, 1099, 1387, 1421, 1508
BpiI GAAGAC 2 cut(s) 457, 872
Bpu1102I GCTNAGC 1 cut(s) 257
Bpu14I TTCGAA 2 cut(s) 1320, 1848
BpuMI CCSGG 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 1780
BsaHI GRCGYC 1 cut(s) 1290
BsaI GGTCTC 2 cut(s) 153, 348
BsaJI CCNNGG 2 cut(s) 486, 936
BsaWI WCCGGW 1 cut(s) 1327
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1862
Bse1I ACTGG 5 cut(s) 799, 1221, 1361, 1380, 1427
Bse3DI GCAATG 2 cut(s) 237, 1202
Bse8I GATNNNNATC 1 cut(s) 1780
BseBI CCWGG 1 cut(s) 1773
BseDI CCNNGG 2 cut(s) 486, 936
BseGI GGATG 5 cut(s) 144, 226, 877, 1255, 1523
BseJI GATNNNNATC 1 cut(s) 1780
BseLI CCNNNNNNNGG 7 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1862
BseMI GCAATG 2 cut(s) 237, 1202
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 5 cut(s) 799, 1221, 1361, 1380, 1427
BseRI GAGGAG 6 cut(s) 42, 89, 573, 647, 1112, 1145
BseXI GCAGC 8 cut(s) 77, 316, 462, 517, 529, 717, 1636, 1695
BsgI GTGCAG 1 cut(s) 1094
BshFI GGCC 2 cut(s) 935, 1771
BsiHKAI GWGCWC 3 cut(s) 34, 402, 806
BsiSI CCGG 3 cut(s) 486, 1286, 1328
BslFI GGGAC 5 cut(s) 299, 1283, 1380, 1521, 1583
BslI CCNNNNNNNGG 7 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1862
BsmAI GTCTC 3 cut(s) 153, 348, 1094
BsmFI GGGAC 5 cut(s) 299, 1283, 1380, 1521, 1583
BsmI GAATGC 1 cut(s) 689
BsnI GGCC 2 cut(s) 935, 1771
Bso31I GGTCTC 2 cut(s) 153, 348
Bsp119I TTCGAA 2 cut(s) 1320, 1848
Bsp1286I GDGCHC 3 cut(s) 34, 402, 806
Bsp1407I TGTACA 1 cut(s) 1225
Bsp143I GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 1 cut(s) 1521
BspANI GGCC 2 cut(s) 935, 1771
BspCNI CTCAG 1 cut(s) 799
BspLI GGNNCC 3 cut(s) 1219, 1509, 1867
BspMI ACCTGC 2 cut(s) 213, 901
BspPI GGATC 3 cut(s) 1212, 1225, 1783
BspT104I TTCGAA 2 cut(s) 1320, 1848
BspTNI GGTCTC 2 cut(s) 153, 348
BsrDI GCAATG 2 cut(s) 237, 1202
BsrGI TGTACA 1 cut(s) 1225
BsrI ACTGG 5 cut(s) 799, 1221, 1361, 1380, 1427
BssECI CCNNGG 2 cut(s) 486, 936
BssMI GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
BssNI GRCGYC 1 cut(s) 1290
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 936
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 1773
Bst4CI ACNGT 7 cut(s) 128, 272, 290, 357, 456, 592, 1406
Bst6I CTCTTC 2 cut(s) 699, 1122
BstACI GRCGYC 1 cut(s) 1290
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 1225
BstBI TTCGAA 2 cut(s) 1320, 1848
BstC8I GCNNGC 2 cut(s) 814, 1713
BstDEI CTNAG 6 cut(s) 257, 347, 807, 1123, 1489, 1898
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 5 cut(s) 144, 226, 877, 1255, 1523
BstH2I RGCGCY 2 cut(s) 476, 655
BstHHI GCGC 3 cut(s) 475, 505, 654
BstKTI GATC 8 cut(s) 969, 1043, 1095, 1137, 1220, 1257, 1390, 1778
BstMAI GTCTC 3 cut(s) 153, 348, 1094
BstMBI GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 1773
BstNSI RCATGY 2 cut(s) 1451, 1730
BstSCI CCNGG 2 cut(s) 485, 1771
BstSFI CTRYAG 2 cut(s) 1209, 1657
BstV1I GCAGC 8 cut(s) 77, 316, 462, 517, 529, 717, 1636, 1695
BstV2I GAAGAC 2 cut(s) 457, 872
BstX2I RGATCY 2 cut(s) 1217, 1775
BstYI RGATCY 2 cut(s) 1217, 1775
BsuRI GGCC 2 cut(s) 935, 1771
BtsCI GGATG 5 cut(s) 144, 226, 877, 1255, 1523
BtsIMutI CAGTG 5 cut(s) 806, 1228, 1368, 1373, 1709
BveI ACCTGC 2 cut(s) 213, 901
Cac8I GCNNGC 2 cut(s) 814, 1713
CaiI CAGNNNCTG 2 cut(s) 572, 1708
CfoI GCGC 3 cut(s) 475, 505, 654
Cfr13I GGNCC 3 cut(s) 1190, 1508, 1753
CseI GACGC 1 cut(s) 127
Csp6I GTAC 6 cut(s) 841, 995, 1163, 1178, 1226, 1895
CviAII CATG 4 cut(s) 301, 1448, 1694, 1727
CviQI GTAC 6 cut(s) 841, 995, 1163, 1178, 1226, 1895
DdeI CTNAG 6 cut(s) 257, 347, 807, 1123, 1489, 1898
DpnI GATC 8 cut(s) 968, 1042, 1094, 1136, 1219, 1256, 1389, 1777
DpnII GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
Eam1104I CTCTTC 2 cut(s) 699, 1122
EarI CTCTTC 2 cut(s) 699, 1122
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 936
Eco147I AGGCCT 1 cut(s) 935
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 2 cut(s) 153, 348
Eco47I GGWCC 3 cut(s) 1190, 1508, 1753
Eco47III AGCGCT 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 534, 1533
EcoICRI GAGCTC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 2 cut(s) 1190, 1508
EcoRI GAATTC 2 cut(s) 1574, 1805
EcoRII CCWGG 1 cut(s) 1771
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 3 cut(s) 1402, 1414, 1449
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 4 cut(s) 304, 1451, 1697, 1730
FalI AAGNNNNNCTT 4 cut(s) 920, 952, 1455, 1487
FaqI GGGAC 5 cut(s) 299, 1283, 1380, 1521, 1583
FatI CATG 4 cut(s) 300, 1447, 1693, 1726
FauI CCCGC 1 cut(s) 1514
FauNDI CATATG 1 cut(s) 278
FbaI TGATCA 1 cut(s) 1254
Fnu4HI GCNGC 8 cut(s) 66, 330, 476, 506, 543, 731, 1625, 1709
FokI GGATG 5 cut(s) 131, 233, 884, 1262, 1530
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 8 cut(s) 66, 330, 476, 506, 543, 731, 1625, 1709
FspBI CTAG 5 cut(s) 122, 252, 309, 584, 1757
GlaI GCGC 3 cut(s) 474, 504, 653
GluI GCNGC 8 cut(s) 66, 330, 476, 506, 543, 731, 1625, 1709
HaeII RGCGCY 2 cut(s) 476, 655
HaeIII GGCC 2 cut(s) 935, 1771
HapII CCGG 3 cut(s) 486, 1286, 1328
HgaI GACGC 1 cut(s) 127
HhaI GCGC 3 cut(s) 475, 505, 654
Hin1I GRCGYC 1 cut(s) 1290
Hin1II CATG 4 cut(s) 304, 1451, 1697, 1730
Hin6I GCGC 3 cut(s) 473, 503, 652
HinP1I GCGC 3 cut(s) 473, 503, 652
HindIII AAGCTT 1 cut(s) 1469
HpaII CCGG 3 cut(s) 486, 1286, 1328
HphI GGTGA 5 cut(s) 788, 1035, 1102, 1349, 1846
Hpy166II GTNNAC 1 cut(s) 1226
Hpy188I TCNGA 8 cut(s) 15, 468, 553, 623, 808, 1276, 1609, 1780
Hpy8I GTNNAC 1 cut(s) 1226
Hpy99I CGWCG 2 cut(s) 121, 1743
HpyAV CCTTC 6 cut(s) 922, 974, 1209, 1358, 1508, 1796
HpyCH4III ACNGT 7 cut(s) 128, 272, 290, 357, 456, 592, 1406
HpyCH4IV ACGT 2 cut(s) 390, 1290
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 6 cut(s) 257, 347, 807, 1123, 1489, 1898
HpySE526I ACGT 2 cut(s) 390, 1290
Hsp92I GRCGYC 1 cut(s) 1290
Hsp92II CATG 4 cut(s) 304, 1451, 1697, 1730
HspAI GCGC 3 cut(s) 473, 503, 652
Ksp22I TGATCA 1 cut(s) 1254
Kzo9I GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
LmnI GCTCC 4 cut(s) 29, 397, 574, 580
Lsp1109I GCAGC 8 cut(s) 77, 316, 462, 517, 529, 717, 1636, 1695
LweI GCATC 6 cut(s) 554, 742, 1099, 1387, 1421, 1508
MaeI CTAG 5 cut(s) 122, 252, 309, 584, 1757
MaeII ACGT 2 cut(s) 390, 1290
MaeIII GTNAC 4 cut(s) 950, 1118, 1484, 1834
MalI GATC 8 cut(s) 968, 1042, 1094, 1136, 1219, 1256, 1389, 1777
MboI GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
MboII GAAGA 9 cut(s) 462, 540, 716, 728, 872, 1139, 1397, 1433, 1585
MflI RGATCY 2 cut(s) 1217, 1775
MhlI GDGCHC 3 cut(s) 34, 402, 806
MlyI GAGTC 4 cut(s) 19, 150, 725, 1265
MmeI TCCRAC 3 cut(s) 339, 438, 1033
Mph1103I ATGCAT 3 cut(s) 1402, 1414, 1449
MroXI GAANNNNTTC 2 cut(s) 879, 1785
MseI TTAA 6 cut(s) 104, 497, 791, 1155, 1302, 1722
MslI CAYNNNNRTG 1 cut(s) 1395
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 3 cut(s) 486, 1286, 1328
MspR9I CCNGG 2 cut(s) 487, 1773
Mva1269I GAATGC 1 cut(s) 689
MvaI CCWGG 1 cut(s) 1773
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 487
NdeI CATATG 1 cut(s) 278
NdeII GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
NlaIII CATG 4 cut(s) 304, 1451, 1697, 1730
NlaIV GGNNCC 3 cut(s) 1219, 1509, 1867
NmuCI GTSAC 2 cut(s) 1484, 1834
NsiI ATGCAT 3 cut(s) 1402, 1414, 1449
NspI RCATGY 2 cut(s) 1451, 1730
NspV TTCGAA 2 cut(s) 1320, 1848
PceI AGGCCT 1 cut(s) 935
PctI GAATGC 1 cut(s) 689
PdmI GAANNNNTTC 2 cut(s) 879, 1785
PfeI GAWTC 7 cut(s) 113, 178, 671, 1021, 1781, 1828, 1845
PflFI GACNNNGTC 1 cut(s) 1737
PflMI CCANNNNNTGG 2 cut(s) 169, 1862
PkrI GCNGC 8 cut(s) 67, 331, 477, 507, 544, 732, 1626, 1710
PleI GAGTC 4 cut(s) 18, 149, 724, 1265
PpsI GAGTC 4 cut(s) 18, 149, 724, 1265
PpuMI RGGWCCY 2 cut(s) 1190, 1508
PsiI TTATAA 1 cut(s) 947
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 2 cut(s) 1190, 1508
Psp6I CCWGG 1 cut(s) 1771
PspGI CCWGG 1 cut(s) 1771
PspN4I GGNNCC 3 cut(s) 1219, 1509, 1867
PspPI GGNCC 3 cut(s) 1190, 1508, 1753
PspPPI RGGWCCY 2 cut(s) 1190, 1508
PstNI CAGNNNCTG 2 cut(s) 572, 1708
PsuI RGATCY 2 cut(s) 1217, 1775
PsyI GACNNNGTC 1 cut(s) 1737
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 6 cut(s) 842, 996, 1164, 1179, 1227, 1896
RsaNI GTAC 6 cut(s) 841, 995, 1163, 1178, 1226, 1895
RseI CAYNNNNRTG 1 cut(s) 1395
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 6 cut(s) 104, 497, 791, 1155, 1302, 1722
SatI GCNGC 8 cut(s) 66, 330, 476, 506, 543, 731, 1625, 1709
Sau3AI GATC 8 cut(s) 966, 1040, 1092, 1134, 1217, 1254, 1387, 1775
Sau96I GGNCC 3 cut(s) 1190, 1508, 1753
SchI GAGTC 4 cut(s) 19, 150, 725, 1265
ScrFI CCNGG 2 cut(s) 487, 1773
SduI GDGCHC 3 cut(s) 34, 402, 806
SfaNI GCATC 6 cut(s) 554, 742, 1099, 1387, 1421, 1508
SfcI CTRYAG 2 cut(s) 1209, 1657
SfuI TTCGAA 2 cut(s) 1320, 1848
SinI GGWCC 3 cut(s) 1190, 1508, 1753
SmiMI CAYNNNNRTG 1 cut(s) 1395
SseBI AGGCCT 1 cut(s) 935
SsiI CCGC 1 cut(s) 1521
SspI AATATT 2 cut(s) 758, 1588
SspMI CTAG 5 cut(s) 122, 252, 309, 584, 1757
SstI GAGCTC 1 cut(s) 402
StuI AGGCCT 1 cut(s) 935
StyD4I CCNGG 2 cut(s) 485, 1771
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 7 cut(s) 128, 272, 290, 357, 456, 592, 1406
TaiI ACGT 2 cut(s) 393, 1293
TaqII GACCGA 1 cut(s) 444
TatI WGTACW 3 cut(s) 1177, 1225, 1894
TfiI GAWTC 7 cut(s) 113, 178, 671, 1021, 1781, 1828, 1845
Tru1I TTAA 6 cut(s) 104, 497, 791, 1155, 1302, 1722
Tru9I TTAA 6 cut(s) 104, 497, 791, 1155, 1302, 1722
TscAI CASTG 5 cut(s) 806, 1228, 1368, 1380, 1709
TseFI GTSAC 2 cut(s) 1484, 1834
TseI GCWGC 8 cut(s) 65, 329, 475, 505, 542, 730, 1624, 1708
Tsp45I GTSAC 2 cut(s) 1484, 1834
TspRI CASTG 5 cut(s) 806, 1228, 1368, 1380, 1709
Tth111I GACNNNGTC 1 cut(s) 1737
Van91I CCANNNNNTGG 2 cut(s) 169, 1862
VpaK11BI GGWCC 3 cut(s) 1190, 1508, 1753
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 5 cut(s) 462, 1574, 1805, 1819, 1850
XceI RCATGY 2 cut(s) 1451, 1730
XcmI CCANNNNNNNNNTGG 1 cut(s) 859
XmnI GAANNNNTTC 2 cut(s) 879, 1785
XspI CTAG 5 cut(s) 122, 252, 309, 584, 1757
ZraI GACGTC 1 cut(s) 1291
Zsp2I ATGCAT 3 cut(s) 1402, 1414, 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.