Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 840 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 561 |
| AatII | GACGTC | 1 cut(s) | 605 |
| Acc36I | ACCTGC | 3 cut(s) | 568, 860, 874 |
| AccB1I | GGYRCC | 1 cut(s) | 184 |
| AccI | GTMKAC | 1 cut(s) | 565 |
| AciI | CCGC | 3 cut(s) | 55, 210, 1055 |
| AclWI | GGATC | 4 cut(s) | 45, 411, 1052, 1065 |
| AcsI | RAATTY | 1 cut(s) | 680 |
| AcyI | GRCGYC | 1 cut(s) | 602 |
| AfaI | GTAC | 3 cut(s) | 509, 688, 975 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 415, 556, 812 |
| AflII | CTTAAG | 1 cut(s) | 929 |
| AgsI | TTSAA | 4 cut(s) | 68, 218, 281, 1026 |
| AjiI | CACGTC | 1 cut(s) | 22 |
| AjnI | CCWGG | 1 cut(s) | 1017 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 17, 49, 620, 652 |
| AluBI | AGCT | 5 cut(s) | 64, 460, 498, 821, 1001 |
| AluI | AGCT | 5 cut(s) | 64, 460, 498, 821, 1001 |
| Alw21I | GWGCWC | 2 cut(s) | 476, 823 |
| Alw26I | GTCTC | 4 cut(s) | 305, 547, 593, 1058 |
| AlwI | GGATC | 4 cut(s) | 45, 411, 1052, 1065 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 248 |
| Ama87I | CYCGRG | 1 cut(s) | 1061 |
| AoxI | GGCC | 1 cut(s) | 141 |
| ApeKI | GCWGC | 3 cut(s) | 109, 155, 158 |
| ApoI | RAATTY | 1 cut(s) | 680 |
| AspS9I | GGNCC | 1 cut(s) | 1182 |
| AsuHPI | GGTGA | 2 cut(s) | 676, 869 |
| AvaI | CYCGRG | 1 cut(s) | 1061 |
| AvaII | GGWCC | 1 cut(s) | 1182 |
| BamHI | GGATCC | 1 cut(s) | 1057 |
| BanI | GGYRCC | 1 cut(s) | 184 |
| BanII | GRGCYC | 1 cut(s) | 823 |
| BbsI | GAAGAC | 1 cut(s) | 1209 |
| Bbv12I | GWGCWC | 2 cut(s) | 476, 823 |
| BbvCI | CCTCAGC | 1 cut(s) | 1002 |
| BbvI | GCAGC | 3 cut(s) | 121, 142, 145 |
| BccI | CCATC | 5 cut(s) | 175, 227, 373, 655, 885 |
| BciT130I | CCWGG | 1 cut(s) | 1019 |
| BciVI | GTATCC | 1 cut(s) | 809 |
| BclI | TGATCA | 2 cut(s) | 666, 760 |
| BcoDI | GTCTC | 4 cut(s) | 305, 547, 593, 1058 |
| BfaI | CTAG | 2 cut(s) | 254, 735 |
| BfmI | CTRYAG | 4 cut(s) | 107, 159, 857, 867 |
| BfrI | CTTAAG | 1 cut(s) | 929 |
| BfuAI | ACCTGC | 3 cut(s) | 568, 860, 874 |
| BfuI | GTATCC | 1 cut(s) | 809 |
| BglII | AGATCT | 1 cut(s) | 334 |
| BisI | GCNGC | 5 cut(s) | 55, 110, 156, 159, 211 |
| BlsI | GCNGC | 5 cut(s) | 56, 111, 157, 160, 212 |
| BmcAI | AGTACT | 1 cut(s) | 688 |
| Bme1390I | CCNGG | 1 cut(s) | 1019 |
| Bme18I | GGWCC | 1 cut(s) | 1182 |
| BmeT110I | CYCGRG | 1 cut(s) | 1061 |
| BmgBI | CACGTC | 1 cut(s) | 22 |
| BmgT120I | GGNCC | 1 cut(s) | 1182 |
| BmiI | GGNNCC | 2 cut(s) | 186, 1059 |
| BmrFI | CCNGG | 1 cut(s) | 1019 |
| BmsI | GCATC | 3 cut(s) | 233, 261, 728 |
| BpiI | GAAGAC | 1 cut(s) | 1209 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 444, 476 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1002 |
| BsaBI | GATNNNNATC | 1 cut(s) | 665 |
| BsaHI | GRCGYC | 1 cut(s) | 602 |
| BsaJI | CCNNGG | 1 cut(s) | 410 |
| BsaWI | WCCGGW | 1 cut(s) | 549 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 114, 144 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 415, 556, 812 |
| Bse1I | ACTGG | 2 cut(s) | 694, 1135 |
| Bse8I | GATNNNNATC | 1 cut(s) | 665 |
| BseBI | CCWGG | 1 cut(s) | 1019 |
| BseDI | CCNNGG | 1 cut(s) | 410 |
| BseGI | GGATG | 4 cut(s) | 276, 544, 595, 1012 |
| BseJI | GATNNNNATC | 1 cut(s) | 665 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 415, 556, 812 |
| BseMII | CTCAG | 3 cut(s) | 560, 916, 993 |
| BseNI | ACTGG | 2 cut(s) | 694, 1135 |
| BseRI | GAGGAG | 3 cut(s) | 180, 389, 831 |
| BseXI | GCAGC | 3 cut(s) | 121, 142, 145 |
| BshFI | GGCC | 1 cut(s) | 143 |
| BshNI | GGYRCC | 1 cut(s) | 184 |
| BsiHKAI | GWGCWC | 2 cut(s) | 476, 823 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1061 |
| BsiSI | CCGG | 1 cut(s) | 550 |
| BslFI | GGGAC | 4 cut(s) | 32, 253, 259, 947 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 415, 556, 812 |
| BsmAI | GTCTC | 4 cut(s) | 305, 547, 593, 1058 |
| BsmBI | CGTCTC | 2 cut(s) | 305, 593 |
| BsmFI | GGGAC | 4 cut(s) | 32, 253, 259, 947 |
| BsmI | GAATGC | 1 cut(s) | 213 |
| BsnI | GGCC | 1 cut(s) | 143 |
| BsoBI | CYCGRG | 1 cut(s) | 1061 |
| Bsp1286I | GDGCHC | 2 cut(s) | 476, 823 |
| Bsp143I | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| BspACI | CCGC | 3 cut(s) | 55, 210, 1055 |
| BspANI | GGCC | 1 cut(s) | 143 |
| BspCNI | CTCAG | 3 cut(s) | 561, 915, 994 |
| BspLI | GGNNCC | 2 cut(s) | 186, 1059 |
| BspMAI | CTGCAG | 4 cut(s) | 111, 163, 861, 871 |
| BspMI | ACCTGC | 3 cut(s) | 568, 860, 874 |
| BspPI | GGATC | 4 cut(s) | 45, 411, 1052, 1065 |
| BspT107I | GGYRCC | 1 cut(s) | 184 |
| BspTI | CTTAAG | 1 cut(s) | 929 |
| BsrI | ACTGG | 2 cut(s) | 694, 1135 |
| BssECI | CCNNGG | 1 cut(s) | 410 |
| BssMI | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| BssNI | GRCGYC | 1 cut(s) | 602 |
| BssT1I | CCWWGG | 1 cut(s) | 410 |
| Bst2UI | CCWGG | 1 cut(s) | 1019 |
| Bst4CI | ACNGT | 1 cut(s) | 366 |
| Bst6I | CTCTTC | 1 cut(s) | 132 |
| BstACI | GRCGYC | 1 cut(s) | 602 |
| BstAFI | CTTAAG | 1 cut(s) | 929 |
| BstC8I | GCNNGC | 1 cut(s) | 496 |
| BstDEI | CTNAG | 4 cut(s) | 477, 569, 902, 1002 |
| BstF5I | GGATG | 4 cut(s) | 276, 544, 595, 1012 |
| BstKTI | GATC | 7 cut(s) | 40, 337, 406, 669, 725, 763, 1060 |
| BstMAI | GTCTC | 4 cut(s) | 305, 547, 593, 1058 |
| BstMBI | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 257 |
| BstNI | CCWGG | 1 cut(s) | 1019 |
| BstNSI | RCATGY | 1 cut(s) | 1055 |
| BstSCI | CCNGG | 1 cut(s) | 1017 |
| BstSFI | CTRYAG | 4 cut(s) | 107, 159, 857, 867 |
| BstV1I | GCAGC | 3 cut(s) | 121, 142, 145 |
| BstV2I | GAAGAC | 1 cut(s) | 1209 |
| BstX2I | RGATCY | 4 cut(s) | 37, 334, 403, 1057 |
| BstYI | RGATCY | 4 cut(s) | 37, 334, 403, 1057 |
| BsuI | GTATCC | 1 cut(s) | 809 |
| BsuRI | GGCC | 1 cut(s) | 143 |
| BtrI | CACGTC | 1 cut(s) | 22 |
| BtsCI | GGATG | 4 cut(s) | 276, 544, 595, 1012 |
| BtsI | GCAGTG | 1 cut(s) | 441 |
| BtsIMutI | CAGTG | 1 cut(s) | 441 |
| BveI | ACCTGC | 3 cut(s) | 568, 860, 874 |
| Cac8I | GCNNGC | 1 cut(s) | 496 |
| CaiI | CAGNNNCTG | 1 cut(s) | 248 |
| Cfr13I | GGNCC | 1 cut(s) | 1182 |
| Csp6I | GTAC | 3 cut(s) | 508, 687, 974 |
| CviAII | CATG | 2 cut(s) | 956, 1052 |
| CviQI | GTAC | 3 cut(s) | 508, 687, 974 |
| DdeI | CTNAG | 4 cut(s) | 477, 569, 902, 1002 |
| DpnI | GATC | 7 cut(s) | 39, 336, 405, 668, 724, 762, 1059 |
| DpnII | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 561 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 561 |
| Eam1104I | CTCTTC | 1 cut(s) | 132 |
| EarI | CTCTTC | 1 cut(s) | 132 |
| Ecl136II | GAGCTC | 1 cut(s) | 821 |
| Eco130I | CCWWGG | 1 cut(s) | 410 |
| Eco147I | AGGCCT | 1 cut(s) | 143 |
| Eco24I | GRGCYC | 1 cut(s) | 823 |
| Eco47I | GGWCC | 1 cut(s) | 1182 |
| Eco53kI | GAGCTC | 1 cut(s) | 821 |
| Eco88I | CYCGRG | 1 cut(s) | 1061 |
| EcoICRI | GAGCTC | 1 cut(s) | 821 |
| EcoRII | CCWGG | 1 cut(s) | 1017 |
| EcoT14I | CCWWGG | 1 cut(s) | 410 |
| EcoT38I | GRGCYC | 1 cut(s) | 823 |
| ErhI | CCWWGG | 1 cut(s) | 410 |
| Esp3I | CGTCTC | 2 cut(s) | 305, 593 |
| FaeI | CATG | 2 cut(s) | 959, 1055 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1201 |
| FaqI | GGGAC | 4 cut(s) | 32, 253, 259, 947 |
| FatI | CATG | 2 cut(s) | 955, 1051 |
| FauNDI | CATATG | 1 cut(s) | 967 |
| FbaI | TGATCA | 2 cut(s) | 666, 760 |
| FblI | GTMKAC | 1 cut(s) | 565 |
| Fnu4HI | GCNGC | 5 cut(s) | 55, 110, 156, 159, 211 |
| FokI | GGATG | 4 cut(s) | 283, 551, 602, 1019 |
| FriOI | GRGCYC | 1 cut(s) | 823 |
| Fsp4HI | GCNGC | 5 cut(s) | 55, 110, 156, 159, 211 |
| FspBI | CTAG | 2 cut(s) | 254, 735 |
| GluI | GCNGC | 5 cut(s) | 55, 110, 156, 159, 211 |
| HaeIII | GGCC | 1 cut(s) | 143 |
| HapII | CCGG | 1 cut(s) | 550 |
| Hin1I | GRCGYC | 1 cut(s) | 602 |
| Hin1II | CATG | 2 cut(s) | 959, 1055 |
| HincII | GTYRAC | 1 cut(s) | 1049 |
| HindII | GTYRAC | 1 cut(s) | 1049 |
| HinfI | GANTC | 4 cut(s) | 190, 353, 1092, 1145 |
| HpaII | CCGG | 1 cut(s) | 550 |
| HphI | GGTGA | 2 cut(s) | 676, 869 |
| Hpy166II | GTNNAC | 2 cut(s) | 566, 1049 |
| Hpy188I | TCNGA | 6 cut(s) | 803, 905, 922, 1075, 1144, 1165 |
| Hpy188III | TCNNGA | 3 cut(s) | 1061, 1180, 1221 |
| Hpy8I | GTNNAC | 2 cut(s) | 566, 1049 |
| Hpy99I | CGWCG | 2 cut(s) | 607, 798 |
| HpyAV | CCTTC | 4 cut(s) | 19, 194, 292, 835 |
| HpyCH4III | ACNGT | 1 cut(s) | 366 |
| HpyCH4IV | ACGT | 4 cut(s) | 21, 314, 602, 796 |
| HpyCH4V | TGCA | 9 cut(s) | 7, 97, 109, 161, 434, 468, 698, 859, 869 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 257 |
| HpyF3I | CTNAG | 4 cut(s) | 477, 569, 902, 1002 |
| HpySE526I | ACGT | 4 cut(s) | 21, 314, 602, 796 |
| Hsp92I | GRCGYC | 1 cut(s) | 602 |
| Hsp92II | CATG | 2 cut(s) | 959, 1055 |
| Ksp22I | TGATCA | 2 cut(s) | 666, 760 |
| Kzo9I | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| LmnI | GCTCC | 5 cut(s) | 61, 257, 333, 818, 826 |
| Lsp1109I | GCAGC | 3 cut(s) | 121, 142, 145 |
| LweI | GCATC | 3 cut(s) | 233, 261, 728 |
| MaeI | CTAG | 2 cut(s) | 254, 735 |
| MaeII | ACGT | 4 cut(s) | 21, 314, 602, 796 |
| MalI | GATC | 7 cut(s) | 39, 336, 405, 668, 724, 762, 1059 |
| MboI | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| MboII | GAAGA | 4 cut(s) | 80, 149, 637, 1209 |
| MfeI | CAATTG | 1 cut(s) | 86 |
| MflI | RGATCY | 4 cut(s) | 37, 334, 403, 1057 |
| MhlI | GDGCHC | 2 cut(s) | 476, 823 |
| MluCI | AATT | 3 cut(s) | 86, 680, 1037 |
| MlyI | GAGTC | 1 cut(s) | 1086 |
| MmeI | TCCRAC | 2 cut(s) | 826, 1019 |
| MseI | TTAA | 5 cut(s) | 318, 359, 789, 930, 1041 |
| MslI | CAYNNNNRTG | 1 cut(s) | 218 |
| MspA1I | CMGCKG | 1 cut(s) | 1001 |
| MspCI | CTTAAG | 1 cut(s) | 929 |
| MspI | CCGG | 1 cut(s) | 550 |
| MspR9I | CCNGG | 1 cut(s) | 1019 |
| MunI | CAATTG | 1 cut(s) | 86 |
| Mva1269I | GAATGC | 1 cut(s) | 213 |
| MvaI | CCWGG | 1 cut(s) | 1019 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 257 |
| NdeI | CATATG | 1 cut(s) | 967 |
| NdeII | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| NlaIII | CATG | 2 cut(s) | 959, 1055 |
| NlaIV | GGNNCC | 2 cut(s) | 186, 1059 |
| NspI | RCATGY | 1 cut(s) | 1055 |
| PceI | AGGCCT | 1 cut(s) | 143 |
| PctI | GAATGC | 1 cut(s) | 213 |
| PfeI | GAWTC | 3 cut(s) | 190, 353, 1145 |
| PkrI | GCNGC | 5 cut(s) | 56, 111, 157, 160, 212 |
| PleI | GAGTC | 1 cut(s) | 1086 |
| PpsI | GAGTC | 1 cut(s) | 1086 |
| PsiI | TTATAA | 1 cut(s) | 840 |
| Psp124BI | GAGCTC | 1 cut(s) | 823 |
| Psp6I | CCWGG | 1 cut(s) | 1017 |
| PspGI | CCWGG | 1 cut(s) | 1017 |
| PspN4I | GGNNCC | 2 cut(s) | 186, 1059 |
| PspPI | GGNCC | 1 cut(s) | 1182 |
| PstI | CTGCAG | 4 cut(s) | 111, 163, 861, 871 |
| PstNI | CAGNNNCTG | 1 cut(s) | 248 |
| PsuI | RGATCY | 4 cut(s) | 37, 334, 403, 1057 |
| PvuII | CAGCTG | 1 cut(s) | 1001 |
| RsaI | GTAC | 3 cut(s) | 509, 688, 975 |
| RsaNI | GTAC | 3 cut(s) | 508, 687, 974 |
| RseI | CAYNNNNRTG | 1 cut(s) | 218 |
| SacI | GAGCTC | 1 cut(s) | 823 |
| SaqAI | TTAA | 5 cut(s) | 318, 359, 789, 930, 1041 |
| SatI | GCNGC | 5 cut(s) | 55, 110, 156, 159, 211 |
| Sau3AI | GATC | 7 cut(s) | 37, 334, 403, 666, 722, 760, 1057 |
| Sau96I | GGNCC | 1 cut(s) | 1182 |
| SbfI | CCTGCAGG | 1 cut(s) | 871 |
| ScaI | AGTACT | 1 cut(s) | 688 |
| SchI | GAGTC | 1 cut(s) | 1086 |
| ScrFI | CCNGG | 1 cut(s) | 1019 |
| SdaI | CCTGCAGG | 1 cut(s) | 871 |
| SduI | GDGCHC | 2 cut(s) | 476, 823 |
| SfaNI | GCATC | 3 cut(s) | 233, 261, 728 |
| SfcI | CTRYAG | 4 cut(s) | 107, 159, 857, 867 |
| SinI | GGWCC | 1 cut(s) | 1182 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 218 |
| SmlI | CTYRAG | 1 cut(s) | 929 |
| SmoI | CTYRAG | 1 cut(s) | 929 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 871 |
| Sse9I | AATT | 3 cut(s) | 86, 680, 1037 |
| SseBI | AGGCCT | 1 cut(s) | 143 |
| SsiI | CCGC | 3 cut(s) | 55, 210, 1055 |
| SspI | AATATT | 1 cut(s) | 264 |
| SspMI | CTAG | 2 cut(s) | 254, 735 |
| SstI | GAGCTC | 1 cut(s) | 823 |
| StuI | AGGCCT | 1 cut(s) | 143 |
| StyD4I | CCNGG | 1 cut(s) | 1017 |
| StyI | CCWWGG | 1 cut(s) | 410 |
| TaaI | ACNGT | 1 cut(s) | 366 |
| TaiI | ACGT | 4 cut(s) | 24, 317, 605, 799 |
| TaqI | TCGA | 4 cut(s) | 44, 372, 721, 1172 |
| TasI | AATT | 3 cut(s) | 86, 680, 1037 |
| TatI | WGTACW | 1 cut(s) | 686 |
| TauI | GCSGC | 2 cut(s) | 57, 213 |
| TfiI | GAWTC | 3 cut(s) | 190, 353, 1145 |
| Tru1I | TTAA | 5 cut(s) | 318, 359, 789, 930, 1041 |
| Tru9I | TTAA | 5 cut(s) | 318, 359, 789, 930, 1041 |
| TscAI | CASTG | 1 cut(s) | 441 |
| TseI | GCWGC | 3 cut(s) | 109, 155, 158 |
| TspDTI | ATGAA | 7 cut(s) | 213, 692, 759, 817, 962, 984, 1221 |
| TspGWI | ACGGA | 1 cut(s) | 991 |
| TspRI | CASTG | 1 cut(s) | 441 |
| Vha464I | CTTAAG | 1 cut(s) | 929 |
| VpaK11BI | GGWCC | 1 cut(s) | 1182 |
| XapI | RAATTY | 1 cut(s) | 680 |
| XceI | RCATGY | 1 cut(s) | 1055 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1127 |
| XmiI | GTMKAC | 1 cut(s) | 565 |
| XspI | CTAG | 2 cut(s) | 254, 735 |
| ZraI | GACGTC | 1 cut(s) | 603 |
| ZrmI | AGTACT | 1 cut(s) | 688 |