MD02G1022000.v1.1

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000408
Physical Location & Seq
Reverse (-)
1539062 .. 1541475
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1022000.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGCACTGGAGTTTATTGGTGGAACACTGTTTACGCTGCTCTACGATGGCGTCAAGCAAGCGATGAGCAAGAGTGGGACGTTTAAAACCCTTCTCGGAGACCTCAAATCCACGCTAGACTCTTTAGAACCAAGAGACATCCAACAGATTGGAGAGCATAACGTCGAATTGGGTCTCCCAAACGAGGAAATTGAAAGTCTTCAAATGCAAATGAAGGATGGTGTGAAGCTTGTTGGAAAGTTGTCGAACTTGCGCATGTGGAACTACTGCTGCTTGGATGATTACGCTCAACAAATTGTTGAGTTGGATAGGGCGCTTAAAAGATTGTTAATGAAATTGAAGATGCAGGAAGCAAGGGATGTGAAGGAGGTCTTGGTTTTGTCGAGGCAAAACCGGGACAAACTTGATGAAGTTAATAGAAGATTGTTGGATATACGGAAGTTACTGCAGCAGAGAGCGGGGGATGTCGTGGAAGCTCAAGGGAATACCGACACAGTGCCCGAGCAAGCTCAGGGGAATGGCTCGGAACAAGTCACTACGTTTGAGGGAGGCGCCGCTCTACAAGCAACATTTGCAGTGTTGTGGGATGTTGTTATGGAAGTGAAAGACAAGACTACAGTGTTCAAACCTCTCATAGAAAATCTCATGTCCACGCTAGACTCCTTAAGACCGTTAATGGAAGAGATACTAGAATATAGCAAACTACTGGATCGCCAAGACGAGTGTCAAAATTTCAGAATACAAATGGAGAAAGGTGTAGAGCTCGTCCACAAGTGCTCCAAAGTTGGTCTGTGGACTGGTTGCAAAAGTTATAAATACAGAAACAAAATTATTGGATTGGATGAATCTCTTCAGAAATTGTTAGGTATACTGAAAGTGCAGGAAGCAAGAGACGTGAGGGAGACGTTTATTTCAATAAGGAATATAGAGGCAGTGATCAAGAGAATTGAAGAAAGTGGTGTGCTGCAAAATCAAACTCAAGCTGACGGTTGGTGTGCATTGCTGGAACCTTCATTGCCTACAATTGGATCGGATGTGCTAAACATGCAGGGAACGAGTGATGCGAGGGAGACATTGGTTTTGTCCAGCACTACAAAAGCAGGTGTCGAACAAATTGAAGGGATTGGTACAGAACAGACTCCAAGTGAAATTACAGCGGATTCAGCTTCTTCTTTTGGATTGAGCTCTTTTCTTGCCAGTCCGCCTCGTGTGAGTCCTAGATTTTCAATGTCTTTAAAGCTCAGTAGATATGGATCAGTACATCTCACTTTAAAGCAGATTAAAAAAACAACTCGCAATTTCTCTCAATCGCAACAAGTAGGTGAAGGAGGTTTTGGAACTGTATACAAGGCTCGGTTAGATGATGGCCAAGTGGTTTCTATTAAACGTGCAAAGAAGGAACACTTTGAGAATTTGCAAACGGAATTCTGCAGTGAAGCTGAACTGCTTGCCAAAATTGATCATTGGAATATCGTAAAGCTACTAGGTTATGTTGATGAAGGAAATGAGCGTCTTATCATTACGGAGTATGTGCCGAACGGTACTCTTAGAGAACATCTGGATTGTCTGCATGGGAAAGTCCTAGAGTTCAAACCAGCGACTTGA

Protein Analysis

535

Amino Acids

59.95

Weight (kDa)

5.58

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 137 7.8e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 183 - 312 2.6e-26 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 435 - 521 9.3e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 436 - 521 1.1e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 813
AarI CACCTGC 1 cut(s) 1091
Acc16I TGCGCA 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 551
AccBSI CCGCTC 2 cut(s) 458, 557
AccI GTMKAC 2 cut(s) 868, 1344
AciI CCGC 4 cut(s) 458, 555, 1157, 1202
AclWI GGATC 3 cut(s) 717, 1036, 1261
AcoI YGGCCR 1 cut(s) 1366
AcsI RAATTY 3 cut(s) 730, 1411, 1424
AcuI CTGAAG 1 cut(s) 836
AcyI GRCGYC 2 cut(s) 51, 552
AfaI GTAC 3 cut(s) 1129, 1260, 1543
AfiI CCNNNNNNNGG 2 cut(s) 184, 1025
AflII CTTAAG 1 cut(s) 664
AgsI TTSAA 9 cut(s) 194, 203, 340, 625, 915, 950, 1118, 1227, 1591
AjiI CACGTC 1 cut(s) 895
AjuI GAANNNNNNNTTGG 4 cut(s) 356, 388, 1317, 1349
Alw21I GWGCWC 3 cut(s) 765, 779, 1187
Alw26I GTCTC 6 cut(s) 93, 129, 179, 885, 896, 1064
AlwI GGATC 3 cut(s) 717, 1036, 1261
Ama87I CYCGRG 1 cut(s) 500
AoxI GGCC 1 cut(s) 1366
ApeKI GCWGC 4 cut(s) 37, 270, 448, 964
ApoI RAATTY 3 cut(s) 730, 1411, 1424
ArsI GACNNNNNNTTYG 2 cut(s) 1063, 1095
Asp700I GAANNNNTTC 2 cut(s) 198, 849
AspLEI GCGC 3 cut(s) 255, 316, 554
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 1334
AvaI CYCGRG 1 cut(s) 500
BaeGI GKGCMC 1 cut(s) 501
BalI TGGCCA 1 cut(s) 1368
BanI GGYRCC 1 cut(s) 551
BanII GRGCYC 2 cut(s) 765, 1187
BauI CACGAG 1 cut(s) 1206
BbsI GAAGAC 1 cut(s) 191
Bbv12I GWGCWC 3 cut(s) 765, 779, 1187
BbvI GCAGC 4 cut(s) 24, 257, 460, 951
BccI CCATC 3 cut(s) 41, 212, 1358
BclI TGATCA 2 cut(s) 936, 1459
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 6 cut(s) 93, 129, 179, 885, 896, 1064
BfaI CTAG 6 cut(s) 116, 656, 689, 1218, 1484, 1583
BfmI CTRYAG 3 cut(s) 446, 615, 1429
BfoI RGCGCY 2 cut(s) 317, 555
BfrI CTTAAG 1 cut(s) 664
BfuAI ACCTGC 1 cut(s) 1091
BisI GCNGC 5 cut(s) 38, 271, 449, 555, 965
BlsI GCNGC 5 cut(s) 39, 272, 450, 556, 966
Bme1390I CCNGG 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 500
BmgBI CACGTC 1 cut(s) 895
BmiI GGNNCC 2 cut(s) 553, 1008
BmrFI CCNGG 1 cut(s) 395
BmsI GCATC 2 cut(s) 333, 1051
BoxI GACNNNNGTC 1 cut(s) 723
BpiI GAAGAC 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 29
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 2 cut(s) 462, 963
BpuMI CCSGG 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 1252
BsaHI GRCGYC 2 cut(s) 51, 552
BsaI GGTCTC 2 cut(s) 93, 179
BsaXI ACNNNNNCTCC 2 cut(s) 761, 791
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 1025
Bse1I ACTGG 4 cut(s) 12, 711, 802, 1197
Bse3DI GCAATG 2 cut(s) 998, 1013
Bse8I GATNNNNATC 1 cut(s) 1252
BseGI GGATG 8 cut(s) 138, 223, 283, 364, 469, 592, 847, 1039
BseJI GATNNNNATC 1 cut(s) 1252
BseLI CCNNNNNNNGG 2 cut(s) 184, 1025
BseMI GCAATG 2 cut(s) 998, 1013
BseMII CTCAG 2 cut(s) 524, 1255
BseNI ACTGG 4 cut(s) 12, 711, 802, 1197
BseSI GKGCMC 1 cut(s) 501
BseXI GCAGC 4 cut(s) 24, 257, 460, 951
BsgI GTGCAG 1 cut(s) 899
BshFI GGCC 1 cut(s) 1368
BshNI GGYRCC 1 cut(s) 551
BsiHKAI GWGCWC 3 cut(s) 765, 779, 1187
BsiHKCI CYCGRG 1 cut(s) 500
BsiSI CCGG 1 cut(s) 394
BslFI GGGAC 2 cut(s) 91, 410
BslI CCNNNNNNNGG 2 cut(s) 184, 1025
BsmAI GTCTC 6 cut(s) 93, 129, 179, 885, 896, 1064
BsmBI CGTCTC 2 cut(s) 885, 896
BsmFI GGGAC 2 cut(s) 91, 410
BsnI GGCC 1 cut(s) 1368
Bso31I GGTCTC 2 cut(s) 93, 179
BsoBI CYCGRG 1 cut(s) 500
Bsp1286I GDGCHC 4 cut(s) 501, 765, 779, 1187
Bsp143I GATC 5 cut(s) 709, 936, 1028, 1253, 1459
BspACI CCGC 4 cut(s) 458, 555, 1157, 1202
BspANI GGCC 1 cut(s) 1368
BspCNI CTCAG 2 cut(s) 523, 1254
BspLI GGNNCC 2 cut(s) 553, 1008
BspMAI CTGCAG 2 cut(s) 450, 1433
BspMI ACCTGC 1 cut(s) 1091
BspPI GGATC 3 cut(s) 717, 1036, 1261
BspT107I GGYRCC 1 cut(s) 551
BspTI CTTAAG 1 cut(s) 664
BspTNI GGTCTC 2 cut(s) 93, 179
BsrBI CCGCTC 2 cut(s) 458, 557
BsrDI GCAATG 2 cut(s) 998, 1013
BsrI ACTGG 4 cut(s) 12, 711, 802, 1197
BssMI GATC 5 cut(s) 709, 936, 1028, 1253, 1459
BssNAI GTATAC 2 cut(s) 869, 1345
BssNI GRCGYC 2 cut(s) 51, 552
BssSI CACGAG 1 cut(s) 1206
Bst1107I GTATAC 2 cut(s) 869, 1345
Bst2BI CACGAG 1 cut(s) 1206
Bst4CI ACNGT 7 cut(s) 30, 496, 619, 672, 989, 1342, 1541
Bst6I CTCTTC 2 cut(s) 675, 855
BstACI GRCGYC 2 cut(s) 51, 552
BstAFI CTTAAG 1 cut(s) 664
BstAPI GCANNNNNTGC 1 cut(s) 572
BstC8I GCNNGC 3 cut(s) 60, 507, 1449
BstDEI CTNAG 3 cut(s) 510, 1241, 1547
BstF5I GGATG 8 cut(s) 138, 223, 283, 364, 469, 592, 847, 1039
BstH2I RGCGCY 2 cut(s) 317, 555
BstHHI GCGC 3 cut(s) 255, 316, 554
BstKTI GATC 5 cut(s) 712, 939, 1031, 1256, 1462
BstMAI GTCTC 6 cut(s) 93, 129, 179, 885, 896, 1064
BstMBI GATC 5 cut(s) 709, 936, 1028, 1253, 1459
BstMWI GCNNNNNNNGC 4 cut(s) 563, 572, 1045, 1163
BstNSI RCATGY 2 cut(s) 259, 1048
BstPAI GACNNNNGTC 1 cut(s) 723
BstSCI CCNGG 1 cut(s) 393
BstSFI CTRYAG 3 cut(s) 446, 615, 1429
BstSLI GKGCMC 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 24, 257, 460, 951
BstV2I GAAGAC 1 cut(s) 191
BstXI CCANNNNNNTGG 1 cut(s) 149
BstZ17I GTATAC 2 cut(s) 869, 1345
BsuRI GGCC 1 cut(s) 1368
BtgZI GCGATG 1 cut(s) 77
BtrI CACGTC 1 cut(s) 895
BtsCI GGATG 8 cut(s) 138, 223, 283, 364, 469, 592, 847, 1039
BtsI GCAGTG 3 cut(s) 582, 939, 1438
BtsIMutI CAGTG 7 cut(s) 5, 26, 501, 582, 624, 939, 1438
BveI ACCTGC 1 cut(s) 1091
Cac8I GCNNGC 3 cut(s) 60, 507, 1449
CfoI GCGC 3 cut(s) 255, 316, 554
CseI GACGC 2 cut(s) 40, 1499
Csp6I GTAC 3 cut(s) 1128, 1259, 1542
CviAII CATG 4 cut(s) 256, 646, 1045, 1571
CviQI GTAC 3 cut(s) 1128, 1259, 1542
DdeI CTNAG 3 cut(s) 510, 1241, 1547
DinI GGCGCC 1 cut(s) 553
DpnI GATC 5 cut(s) 711, 938, 1030, 1255, 1461
DpnII GATC 5 cut(s) 709, 936, 1028, 1253, 1459
DraI TTTAAA 3 cut(s) 85, 1236, 1272
EaeI YGGCCR 1 cut(s) 1366
Eam1104I CTCTTC 2 cut(s) 675, 855
EarI CTCTTC 2 cut(s) 675, 855
EciI GGCGGA 1 cut(s) 1191
Ecl136II GAGCTC 2 cut(s) 763, 1185
Eco24I GRGCYC 2 cut(s) 765, 1187
Eco31I GGTCTC 2 cut(s) 93, 179
Eco53kI GAGCTC 2 cut(s) 763, 1185
Eco57I CTGAAG 1 cut(s) 836
Eco88I CYCGRG 1 cut(s) 500
EcoICRI GAGCTC 2 cut(s) 763, 1185
EcoRI GAATTC 1 cut(s) 1424
EcoT38I GRGCYC 2 cut(s) 765, 1187
EgeI GGCGCC 1 cut(s) 553
EheI GGCGCC 1 cut(s) 553
Esp3I CGTCTC 2 cut(s) 885, 896
FaeI CATG 4 cut(s) 259, 649, 1048, 1574
FalI AAGNNNNNCTT 4 cut(s) 356, 388, 1388, 1420
FaqI GGGAC 2 cut(s) 91, 410
FatI CATG 4 cut(s) 255, 645, 1044, 1570
FauI CCCGC 1 cut(s) 451
FbaI TGATCA 2 cut(s) 936, 1459
FblI GTMKAC 2 cut(s) 868, 1344
Fnu4HI GCNGC 5 cut(s) 38, 271, 449, 555, 965
FokI GGATG 8 cut(s) 125, 230, 290, 371, 476, 599, 854, 1046
FriOI GRGCYC 2 cut(s) 765, 1187
Fsp4HI GCNGC 5 cut(s) 38, 271, 449, 555, 965
FspBI CTAG 6 cut(s) 116, 656, 689, 1218, 1484, 1583
FspI TGCGCA 1 cut(s) 254
GlaI GCGC 3 cut(s) 254, 315, 553
GluI GCNGC 5 cut(s) 38, 271, 449, 555, 965
GsuI CTGGAG 1 cut(s) 29
HaeII RGCGCY 2 cut(s) 317, 555
HaeIII GGCC 1 cut(s) 1368
HapII CCGG 1 cut(s) 394
HgaI GACGC 2 cut(s) 40, 1499
HhaI GCGC 3 cut(s) 255, 316, 554
Hin1I GRCGYC 2 cut(s) 51, 552
Hin1II CATG 4 cut(s) 259, 649, 1048, 1574
Hin6I GCGC 3 cut(s) 253, 314, 552
HinP1I GCGC 3 cut(s) 253, 314, 552
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 6 cut(s) 119, 659, 845, 1138, 1160, 1213
HpaII CCGG 1 cut(s) 394
HphI GGTGA 1 cut(s) 1334
Hpy166II GTNNAC 6 cut(s) 33, 651, 769, 795, 869, 1345
Hpy188I TCNGA 5 cut(s) 98, 526, 737, 855, 1033
Hpy188III TCNNGA 2 cut(s) 940, 1559
Hpy8I GTNNAC 6 cut(s) 33, 651, 769, 795, 869, 1345
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 8 cut(s) 101, 208, 358, 1020, 1112, 1319, 1390, 1493
HpyCH4III ACNGT 7 cut(s) 30, 496, 619, 672, 989, 1342, 1541
HpyCH4IV ACGT 6 cut(s) 80, 162, 539, 894, 905, 1387
HpyF10VI GCNNNNNNNGC 4 cut(s) 563, 572, 1045, 1163
HpyF3I CTNAG 3 cut(s) 510, 1241, 1547
HpySE526I ACGT 6 cut(s) 80, 162, 539, 894, 905, 1387
Hsp92I GRCGYC 2 cut(s) 51, 552
Hsp92II CATG 4 cut(s) 259, 649, 1048, 1574
HspAI GCGC 3 cut(s) 253, 314, 552
KasI GGCGCC 1 cut(s) 551
Ksp22I TGATCA 2 cut(s) 936, 1459
Kzo9I GATC 5 cut(s) 709, 936, 1028, 1253, 1459
LmnI GCTCC 1 cut(s) 782
Lsp1109I GCAGC 4 cut(s) 24, 257, 460, 951
LweI GCATC 2 cut(s) 333, 1051
MaeI CTAG 6 cut(s) 116, 656, 689, 1218, 1484, 1583
MaeII ACGT 6 cut(s) 80, 162, 539, 894, 905, 1387
MaeIII GTNAC 2 cut(s) 441, 532
MalI GATC 5 cut(s) 711, 938, 1030, 1255, 1461
MbiI CCGCTC 2 cut(s) 458, 557
MboI GATC 5 cut(s) 709, 936, 1028, 1253, 1459
MboII GAAGA 7 cut(s) 191, 352, 432, 692, 842, 962, 1161
MfeI CAATTG 1 cut(s) 1023
MhlI GDGCHC 4 cut(s) 501, 765, 779, 1187
MlsI TGGCCA 1 cut(s) 1368
MluNI TGGCCA 1 cut(s) 1368
Mly113I GGCGCC 1 cut(s) 552
MlyI GAGTC 4 cut(s) 113, 653, 1132, 1222
MmeI TCCRAC 4 cut(s) 166, 214, 285, 408
Mox20I TGGCCA 1 cut(s) 1368
MroXI GAANNNNTTC 2 cut(s) 198, 849
MscI TGGCCA 1 cut(s) 1368
Msp20I TGGCCA 1 cut(s) 1368
MspA1I CMGCKG 1 cut(s) 1157
MspCI CTTAAG 1 cut(s) 664
MspI CCGG 1 cut(s) 394
MspR9I CCNGG 1 cut(s) 395
MunI CAATTG 1 cut(s) 1023
MwoI GCNNNNNNNGC 4 cut(s) 563, 572, 1045, 1163
NarI GGCGCC 1 cut(s) 552
NciI CCSGG 1 cut(s) 395
NdeII GATC 5 cut(s) 709, 936, 1028, 1253, 1459
NlaIII CATG 4 cut(s) 259, 649, 1048, 1574
NlaIV GGNNCC 2 cut(s) 553, 1008
NmuCI GTSAC 1 cut(s) 532
NsbI TGCGCA 1 cut(s) 254
NspI RCATGY 2 cut(s) 259, 1048
PaqCI CACCTGC 1 cut(s) 1091
PcsI WCGNNNNNNNCGW 1 cut(s) 1061
PdmI GAANNNNTTC 2 cut(s) 198, 849
PfeI GAWTC 2 cut(s) 845, 1160
PkrI GCNGC 5 cut(s) 39, 272, 450, 556, 966
PleI GAGTC 4 cut(s) 113, 653, 1132, 1221
PluTI GGCGCC 1 cut(s) 555
PpsI GAGTC 4 cut(s) 113, 653, 1132, 1221
PshAI GACNNNNGTC 1 cut(s) 723
PsiI TTATAA 1 cut(s) 813
Psp124BI GAGCTC 2 cut(s) 765, 1187
PspN4I GGNNCC 2 cut(s) 553, 1008
PsrI GAACNNNNNNTAC 2 cut(s) 16, 48
PstI CTGCAG 2 cut(s) 450, 1433
RsaI GTAC 3 cut(s) 1129, 1260, 1543
RsaNI GTAC 3 cut(s) 1128, 1259, 1542
SacI GAGCTC 2 cut(s) 765, 1187
SatI GCNGC 5 cut(s) 38, 271, 449, 555, 965
Sau3AI GATC 5 cut(s) 709, 936, 1028, 1253, 1459
SchI GAGTC 4 cut(s) 113, 653, 1132, 1222
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 4 cut(s) 501, 765, 779, 1187
SfaNI GCATC 2 cut(s) 333, 1051
SfcI CTRYAG 3 cut(s) 446, 615, 1429
SfoI GGCGCC 1 cut(s) 553
SmlI CTYRAG 3 cut(s) 477, 664, 978
SmoI CTYRAG 3 cut(s) 477, 664, 978
SsiI CCGC 4 cut(s) 458, 555, 1157, 1202
SspDI GGCGCC 1 cut(s) 551
SspMI CTAG 6 cut(s) 116, 656, 689, 1218, 1484, 1583
SstI GAGCTC 2 cut(s) 765, 1187
StyD4I CCNGG 1 cut(s) 393
TaaI ACNGT 7 cut(s) 30, 496, 619, 672, 989, 1342, 1541
TaiI ACGT 6 cut(s) 83, 165, 542, 897, 908, 1390
TaqI TCGA 4 cut(s) 165, 245, 383, 1107
TatI WGTACW 1 cut(s) 1258
TauI GCSGC 1 cut(s) 557
TfiI GAWTC 2 cut(s) 845, 1160
TscAI CASTG 7 cut(s) 12, 33, 501, 582, 624, 939, 1438
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 4 cut(s) 37, 270, 448, 964
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 6 cut(s) 227, 347, 423, 858, 1002, 1512
TspGWI ACGGA 3 cut(s) 451, 1436, 1538
TspRI CASTG 7 cut(s) 12, 33, 501, 582, 624, 939, 1438
Vha464I CTTAAG 1 cut(s) 664
XapI RAATTY 3 cut(s) 730, 1411, 1424
XceI RCATGY 2 cut(s) 259, 1048
XmiI GTMKAC 2 cut(s) 868, 1344
XmnI GAANNNNTTC 2 cut(s) 198, 849
XspI CTAG 6 cut(s) 116, 656, 689, 1218, 1484, 1583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.