Rh7DG083400
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000408
Physical Location & Seq
Forward (+)
6636088 .. 6639992
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG083400.1

Sequence Viewer

Length: 2271 bp
ATGTCTGAGTTATTGAAAGTGCATCAGGAAGTTCTGAAAGCGCAGAAAGAAATGTTTGATATGATGAGAGTGCAGCATGAGGCTGTACATGAGGCAATGCAGAGAATTAGAGCTGATGCTGTGCAAAATGTGCAAGGAGAAACTGCCCCTGCTCCAGCTCCAGCTCCAGCTCTAAGAATAGTATTTTGGGTGCTGTATGATGTTGTTATACAAGTGAAGGTCAAGAATATAATGTGTAAACGCCTCCTTCAAGAATTCAAATCCACGCTCGACTTCTTAAAACCACTGATAGAAGAAATGGCAGAAAGTAACAAACTATTGCATCTTCCAGAGCAGGAACAAGAGAAGTTTATTATTCAAATGAGGGAGGGTGTCGAGCTCATTGGCAAGTGCTCCAAGGTTAGCAAGTGGCATAGTTACAAAAAGTATGCATGCACCAACAAACTTCTTAAATTAGATGAATCTCTCCGGGGGCTATTCCATAAAATGAGTATGCAGATTGCAATCCATGTGAAGGAAGGTTTGATTTCGATAAGTAGTATGGAGGCAATGATCAAGAAAATTGAAGAGAGTGGTGTGATACAAAATCAAAGTCAAGTTGTACTCACTAAACCACAACCTCCACTGCATGAAGCTGGATTGGATGTTCTGGATACGCATGGAAGTAAGGATGTGGAGGGAACAGGAGATGATTTAAATGAGAAAGAGGTTGGCGTGAAGCAAATTGGAAGAGATGGTACGAGACAGAATGAAAAAGAGATTGAAGGTTTGAGTGAAGTACCTGAAAATTCATCCCCTAAAACTACATTGGATGTGCAAGGAACAAAGGATATAAATGAGTCACTAGATTCAGAAAGTGTACATATAGCGCAGACCAAGCAAAGTCAAGGGACAAGGATGGTTGAAGCTCCAGTTGCTGAGTCATATAAATTTCCATTCCTTATGGTTGAATCGGATGTGTATTTGAGGGAATTAAAGAAGAAGATTTTTGAGGATCAGGTGGCGATGCTTGTGATTACAGGTCCAGCAGGATGCGGGAAAACCACACTGGCAAGAATGTTTTGTGAAGATCAGGAGGTCGTGGCGGCCGTGAGAGCTACAGGGGACTACCCAGAAAGAGTGGGCAAACCTATATGTGAGTATTATTTAAAGACTGGGACCTGTAAATTTGGTCCGTCCTGCAAGTTCCATCATCCGAAACAGGGAATTGGATACTTGAGCTCTTTGTATGGACTGACACAGCTATCTTCTCCCACCCATGCCCTTGAAAGACCTTATACCTCTGTCGTACCATCATCAGTTGGCCCTTCTAGCAGCAGCCCAAATGAACAAGCCTTCCCAGAGAGGCCTAGAGAACCTGAATGCCAGCATTACCTGAGAACTGGATACTATAAATTTGTACCCTCATGTCGGTACCACCATCCTCGAGATCAGATTGCACCAAGGACAAATTGTCTCCTCAGTCCAGTTGGTCTTCTTGGCAATTCAGGCTCTAATGCTTGGCCTTCATTCAGTCAAGTGTCATTGTCAATGGAAGATTTTCAGACGTCTTTGGATTTGAAATTGAATGCTCAGCATAGCCTTCTCCAGACACCAATGTCACCTTGTGTATCCATAAGTCGAAGAATAAGCAGCAATGACAAGTCCAGTCAGTCAGATGTAGAGGATAAGAGTGAGCTTGATGAGATTCAAAGCCAAATTTCAGGTGTCACTGTTCCGGAGTCTCTACATGATCCACTTGAAAGTAAAGATCAATGTCCAATGAATGTGAACAACACTTTGGAATATGCAGCTTCAGGGACAACTCCGAATTCAGTTAACGTTGATGGTCAGTCTGTTACTTGTAACAGCATAGGTGAGATTGGAAGCATATCACAGAAGCAGGGAGTTAAAGAAAAAGTTGCATTTAGAACAAAATCAGAGATCGAGATATTGGATGATGGATTCAAATGGAGGAAGTATGGGAAAAAATTTGGGAAGAACAGCACAAATCCAAGGAATTACTATAAGTGTGCATATGAAGGCTGCCCAGTGAAAAAGAGAGTTGAAAGAGATGGACAAGATCCAAGTTATGTAATTACGTGTTATAACGGGGTTCATAACCATACAAGCCTTGGCAAAAGAACCCAGCGAGTACCCGCCAGCCACCCTCAACCTCTCCCTTTTCCCCTCCCTATAGGGAAGGAAGAGAGCTTTACGTTCGATCATCTTGCAGACGATAATCAGGAAGAGAGCGTTCTGTTCGATCATCTTGCAGACCATATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

756

Amino Acids

84.55

Weight (kDa)

6.15

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 56 - 186 4.7e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
zf-CCCH PF00642 376 - 400 7.4e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
WRKY PF03106 646 - 702 9.5e-22 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2088
AasI GACNNNNNNGTC 1 cut(s) 1597
AatII GACGTC 1 cut(s) 1550
Acc65I GGTACC 1 cut(s) 1413
AccB1I GGYRCC 1 cut(s) 1413
AccIII TCCGGA 1 cut(s) 1717
AciI CCGC 3 cut(s) 1035, 1085, 2139
AclI AACGTT 1 cut(s) 1821
AclWI GGATC 3 cut(s) 1002, 1727, 2057
AcoI YGGCCR 1 cut(s) 1086
AcsI RAATTY 8 cut(s) 254, 787, 929, 1166, 1394, 1698, 1810, 1970
AcuI CTGAAG 1 cut(s) 1779
AcyI GRCGYC 1 cut(s) 1547
AdeI CACNNNGTG 1 cut(s) 1607
AfaI GTAC 9 cut(s) 87, 603, 739, 780, 861, 1290, 1401, 1415, 2136
AfiI CCNNNNNNNGG 3 cut(s) 514, 1202, 1410
AflIII ACRYGT 1 cut(s) 2081
AhdI GACNNNNNGTC 1 cut(s) 1452
AjuI GAANNNNNNNTTGG 4 cut(s) 169, 201, 1763, 1795
AloI GAACNNNNNNTCC 4 cut(s) 630, 662, 1320, 1352
Alw21I GWGCWC 3 cut(s) 381, 395, 1223
Alw26I GTCTC 3 cut(s) 736, 1460, 1728
AlwI GGATC 3 cut(s) 1002, 1727, 2057
AlwNI CAGNNNCTG 1 cut(s) 917
Ama87I CYCGRG 1 cut(s) 1425
Aor13HI TCCGGA 1 cut(s) 1717
AoxI GGCC 4 cut(s) 1086, 1303, 1346, 1502
ApeKI GCWGC 6 cut(s) 73, 1314, 1317, 1632, 1790, 2025
ApoI RAATTY 8 cut(s) 254, 787, 929, 1166, 1394, 1698, 1810, 1970
ArsI GACNNNNNNTTYG 2 cut(s) 577, 609
Asp700I GAANNNNTTC 1 cut(s) 1539
Asp718I GGTACC 1 cut(s) 1413
AspLEI GCGC 2 cut(s) 43, 871
AspS9I GGNCC 4 cut(s) 1022, 1158, 1172, 1304
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 1593, 1868
AvaI CYCGRG 1 cut(s) 1425
AvaII GGWCC 3 cut(s) 1022, 1158, 1172
BanI GGYRCC 1 cut(s) 1413
BanII GRGCYC 2 cut(s) 381, 1223
BarI GAAGNNNNNNTAC 2 cut(s) 721, 753
BbsI GAAGAC 1 cut(s) 1466
Bbv12I GWGCWC 3 cut(s) 381, 395, 1223
BbvI GCAGC 6 cut(s) 85, 1326, 1329, 1644, 1802, 2012
BccI CCATC 8 cut(s) 728, 892, 1197, 1300, 1428, 1820, 1934, 2048
BceAI ACGGC 1 cut(s) 1073
BcgI CGANNNNNNTGC 4 cut(s) 2192, 2226, 2234, 2268
BciVI GTATCC 4 cut(s) 646, 1205, 1379, 1621
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 3 cut(s) 736, 1460, 1728
BfaI CTAG 3 cut(s) 845, 1311, 1350
BfmI CTRYAG 2 cut(s) 1098, 2175
BfuI GTATCC 4 cut(s) 646, 1205, 1379, 1621
BisI GCNGC 7 cut(s) 74, 1086, 1315, 1318, 1633, 1791, 2026
BlpI GCTNAGC 1 cut(s) 1572
BlsI GCNGC 7 cut(s) 75, 1087, 1316, 1319, 1634, 1792, 2027
Bme1390I CCNGG 1 cut(s) 470
Bme18I GGWCC 3 cut(s) 1022, 1158, 1172
BmeRI GACNNNNNGTC 1 cut(s) 1452
BmeT110I CYCGRG 1 cut(s) 1425
BmgT120I GGNCC 4 cut(s) 1022, 1158, 1172, 1304
BmiI GGNNCC 2 cut(s) 1159, 1415
BmrFI CCNGG 1 cut(s) 470
BmrI ACTGGG 2 cut(s) 1164, 2024
BmsI GCATC 5 cut(s) 31, 106, 331, 996, 1022
BmuI ACTGGG 2 cut(s) 1164, 2024
BpiI GAAGAC 1 cut(s) 1466
BpmI CTGGAG 5 cut(s) 138, 144, 150, 894, 1571
Bpu1102I GCTNAGC 1 cut(s) 1572
BpuEI CTTGAG 1 cut(s) 1237
BpuMI CCSGG 1 cut(s) 470
BsaAI YACGTR 1 cut(s) 2082
BsaBI GATNNNNATC 1 cut(s) 503
BsaHI GRCGYC 1 cut(s) 1547
BsaJI CCNNGG 5 cut(s) 396, 469, 1442, 1994, 2113
BsaWI WCCGGW 1 cut(s) 1717
Bsc4I CCNNNNNNNGG 3 cut(s) 514, 1202, 1410
Bse1I ACTGG 7 cut(s) 911, 1053, 1159, 1387, 1466, 1647, 2030
Bse3DI GCAATG 3 cut(s) 102, 555, 1642
Bse8I GATNNNNATC 1 cut(s) 503
BseAI TCCGGA 1 cut(s) 1717
BseDI CCNNGG 5 cut(s) 396, 469, 1442, 1994, 2113
BseJI GATNNNNATC 1 cut(s) 503
BseLI CCNNNNNNNGG 3 cut(s) 514, 1202, 1410
BseMI GCAATG 3 cut(s) 102, 555, 1642
BseMII CTCAG 4 cut(s) 909, 1367, 1474, 1586
BseNI ACTGG 7 cut(s) 911, 1053, 1159, 1387, 1466, 1647, 2030
BseRI GAGGAG 1 cut(s) 1448
BseX3I CGGCCG 1 cut(s) 1086
BseXI GCAGC 6 cut(s) 85, 1326, 1329, 1644, 1802, 2012
BseYI CCCAGC 1 cut(s) 2127
BsgI GTGCAG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 1089
BshFI GGCC 4 cut(s) 1088, 1305, 1348, 1504
BshNI GGYRCC 1 cut(s) 1413
BsiEI CGRYCG 1 cut(s) 1089
BsiHKAI GWGCWC 3 cut(s) 381, 395, 1223
BsiHKCI CYCGRG 1 cut(s) 1425
BsiSI CCGG 2 cut(s) 469, 1718
BslFI GGGAC 4 cut(s) 904, 1118, 1171, 1813
BslI CCNNNNNNNGG 3 cut(s) 514, 1202, 1410
BsmAI GTCTC 3 cut(s) 736, 1460, 1728
BsmFI GGGAC 4 cut(s) 904, 1118, 1171, 1813
BsmI GAATGC 2 cut(s) 1367, 1573
BsnI GGCC 4 cut(s) 1088, 1305, 1348, 1504
BsoBI CYCGRG 1 cut(s) 1425
Bsp1286I GDGCHC 3 cut(s) 381, 395, 1223
Bsp13I TCCGGA 1 cut(s) 1717
Bsp1407I TGTACA 2 cut(s) 85, 859
Bsp1720I GCTNAGC 1 cut(s) 1572
BspACI CCGC 3 cut(s) 1035, 1085, 2139
BspANI GGCC 4 cut(s) 1088, 1305, 1348, 1504
BspCNI CTCAG 4 cut(s) 910, 1368, 1473, 1585
BspEI TCCGGA 1 cut(s) 1717
BspLI GGNNCC 2 cut(s) 1159, 1415
BspPI GGATC 3 cut(s) 1002, 1727, 2057
BspT107I GGYRCC 1 cut(s) 1413
BsrDI GCAATG 3 cut(s) 102, 555, 1642
BsrGI TGTACA 2 cut(s) 85, 859
BsrI ACTGG 7 cut(s) 911, 1053, 1159, 1387, 1466, 1647, 2030
BssECI CCNNGG 5 cut(s) 396, 469, 1442, 1994, 2113
BssNI GRCGYC 1 cut(s) 1547
BssT1I CCWWGG 4 cut(s) 396, 1442, 1994, 2113
Bst4CI ACNGT 1 cut(s) 1714
Bst6I CTCTTC 4 cut(s) 561, 724, 2181, 2223
BstACI GRCGYC 1 cut(s) 1547
BstAPI GCANNNNNTGC 1 cut(s) 130
BstAUI TGTACA 2 cut(s) 85, 859
BstBAI YACGTR 1 cut(s) 2082
BstC8I GCNNGC 3 cut(s) 433, 1367, 2143
BstDEI CTNAG 6 cut(s) 6, 173, 918, 1376, 1460, 1572
BstHHI GCGC 2 cut(s) 43, 871
BstMAI GTCTC 3 cut(s) 736, 1460, 1728
BstMCI CGRYCG 1 cut(s) 1089
BstMWI GCNNNNNNNGC 6 cut(s) 130, 877, 914, 1094, 1311, 1488
BstNSI RCATGY 1 cut(s) 435
BstSCI CCNGG 1 cut(s) 468
BstSFI CTRYAG 2 cut(s) 1098, 2175
BstV1I GCAGC 6 cut(s) 85, 1326, 1329, 1644, 1802, 2012
BstV2I GAAGAC 1 cut(s) 1466
BstX2I RGATCY 1 cut(s) 2062
BstYI RGATCY 1 cut(s) 2062
BstZI CGGCCG 1 cut(s) 1086
BsuI GTATCC 4 cut(s) 646, 1205, 1379, 1621
BsuRI GGCC 4 cut(s) 1088, 1305, 1348, 1504
BtgZI GCGATG 1 cut(s) 1019
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 5 cut(s) 284, 623, 1046, 1710, 2037
Cac8I GCNNGC 3 cut(s) 433, 1367, 2143
CaiI CAGNNNCTG 1 cut(s) 917
CfoI GCGC 2 cut(s) 43, 871
Cfr13I GGNCC 4 cut(s) 1022, 1158, 1172, 1304
Csp6I GTAC 9 cut(s) 86, 602, 738, 779, 860, 1289, 1400, 1414, 2135
CviAII CATG 9 cut(s) 77, 89, 432, 509, 629, 659, 1259, 1407, 1730
CviQI GTAC 9 cut(s) 86, 602, 738, 779, 860, 1289, 1400, 1414, 2135
DdeI CTNAG 6 cut(s) 6, 173, 918, 1376, 1460, 1572
DraI TTTAAA 2 cut(s) 696, 1149
DraIII CACNNNGTG 1 cut(s) 1607
DrdI GACNNNNNNGTC 1 cut(s) 1597
DriI GACNNNNNGTC 1 cut(s) 1452
DseDI GACNNNNNNGTC 1 cut(s) 1597
EaeI YGGCCR 1 cut(s) 1086
EagI CGGCCG 1 cut(s) 1086
Eam1104I CTCTTC 4 cut(s) 561, 724, 2181, 2223
Eam1105I GACNNNNNGTC 1 cut(s) 1452
EarI CTCTTC 4 cut(s) 561, 724, 2181, 2223
Ecl136II GAGCTC 2 cut(s) 379, 1221
EclXI CGGCCG 1 cut(s) 1086
Eco130I CCWWGG 4 cut(s) 396, 1442, 1994, 2113
Eco147I AGGCCT 1 cut(s) 1348
Eco24I GRGCYC 2 cut(s) 381, 1223
Eco47I GGWCC 3 cut(s) 1022, 1158, 1172
Eco52I CGGCCG 1 cut(s) 1086
Eco53kI GAGCTC 2 cut(s) 379, 1221
Eco57I CTGAAG 1 cut(s) 1779
Eco88I CYCGRG 1 cut(s) 1425
EcoICRI GAGCTC 2 cut(s) 379, 1221
EcoO109I RGGNCCY 1 cut(s) 1158
EcoRI GAATTC 2 cut(s) 254, 1810
EcoT14I CCWWGG 4 cut(s) 396, 1442, 1994, 2113
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 2 cut(s) 381, 1223
ErhI CCWWGG 4 cut(s) 396, 1442, 1994, 2113
FaeI CATG 9 cut(s) 80, 92, 435, 512, 632, 662, 1262, 1410, 1733
FaqI GGGAC 4 cut(s) 904, 1118, 1171, 1813
FatI CATG 9 cut(s) 76, 88, 431, 508, 628, 658, 1258, 1406, 1729
FauI CCCGC 2 cut(s) 1028, 2146
FauNDI CATATG 1 cut(s) 2017
FbaI TGATCA 1 cut(s) 552
Fnu4HI GCNGC 7 cut(s) 74, 1086, 1315, 1318, 1633, 1791, 2026
FriOI GRGCYC 2 cut(s) 381, 1223
Fsp4HI GCNGC 7 cut(s) 74, 1086, 1315, 1318, 1633, 1791, 2026
FspBI CTAG 3 cut(s) 845, 1311, 1350
GlaI GCGC 2 cut(s) 42, 870
GluI GCNGC 7 cut(s) 74, 1086, 1315, 1318, 1633, 1791, 2026
GsaI CCCAGC 1 cut(s) 2131
GsuI CTGGAG 5 cut(s) 138, 144, 150, 894, 1571
HaeIII GGCC 4 cut(s) 1088, 1305, 1348, 1504
HapII CCGG 2 cut(s) 469, 1718
HhaI GCGC 2 cut(s) 43, 871
Hin1I GRCGYC 1 cut(s) 1547
Hin1II CATG 9 cut(s) 80, 92, 435, 512, 632, 662, 1262, 1410, 1733
Hin6I GCGC 2 cut(s) 41, 869
HinP1I GCGC 2 cut(s) 41, 869
HincII GTYRAC 1 cut(s) 1819
HindII GTYRAC 1 cut(s) 1819
HinfI GANTC 8 cut(s) 461, 839, 848, 920, 950, 1687, 1721, 1944
HpaI GTTAAC 1 cut(s) 1819
HpaII CCGG 2 cut(s) 469, 1718
HphI GGTGA 2 cut(s) 1593, 1868
Hpy166II GTNNAC 4 cut(s) 239, 860, 1771, 1819
Hpy8I GTNNAC 4 cut(s) 239, 860, 1771, 1819
HpyCH4III ACNGT 1 cut(s) 1714
HpyCH4IV ACGT 4 cut(s) 1547, 1821, 2081, 2198
HpyF10VI GCNNNNNNNGC 6 cut(s) 130, 877, 914, 1094, 1311, 1488
HpyF3I CTNAG 6 cut(s) 6, 173, 918, 1376, 1460, 1572
HpySE526I ACGT 4 cut(s) 1547, 1821, 2081, 2198
Hsp92I GRCGYC 1 cut(s) 1547
Hsp92II CATG 9 cut(s) 80, 92, 435, 512, 632, 662, 1262, 1410, 1733
HspAI GCGC 2 cut(s) 41, 869
Kpn2I TCCGGA 1 cut(s) 1717
KpnI GGTACC 1 cut(s) 1417
Ksp22I TGATCA 1 cut(s) 552
KspAI GTTAAC 1 cut(s) 1819
LmnI GCTCC 5 cut(s) 157, 163, 169, 398, 913
Lsp1109I GCAGC 6 cut(s) 85, 1326, 1329, 1644, 1802, 2012
LweI GCATC 5 cut(s) 31, 106, 331, 996, 1022
MaeI CTAG 3 cut(s) 845, 1311, 1350
MaeII ACGT 4 cut(s) 1547, 1821, 2081, 2198
MaeIII GTNAC 7 cut(s) 308, 416, 840, 1599, 1708, 1837, 1844
MflI RGATCY 1 cut(s) 2062
MhlI GDGCHC 3 cut(s) 381, 395, 1223
MlyI GAGTC 3 cut(s) 848, 929, 1730
Mph1103I ATGCAT 1 cut(s) 433
MroI TCCGGA 1 cut(s) 1717
MroXI GAANNNNTTC 1 cut(s) 1539
MseI TTAA 7 cut(s) 278, 450, 695, 974, 1148, 1818, 1890
MspI CCGG 2 cut(s) 469, 1718
MspR9I CCNGG 1 cut(s) 470
Mva1269I GAATGC 2 cut(s) 1367, 1573
MwoI GCNNNNNNNGC 6 cut(s) 130, 877, 914, 1094, 1311, 1488
NciI CCSGG 1 cut(s) 470
NdeI CATATG 1 cut(s) 2017
NlaIII CATG 9 cut(s) 80, 92, 435, 512, 632, 662, 1262, 1410, 1733
NlaIV GGNNCC 2 cut(s) 1159, 1415
NmuCI GTSAC 3 cut(s) 840, 1599, 1708
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 1 cut(s) 435
PaeI GCATGC 1 cut(s) 435
PaeR7I CTCGAG 1 cut(s) 1425
PceI AGGCCT 1 cut(s) 1348
PcsI WCGNNNNNNNCGW 1 cut(s) 1086
PctI GAATGC 2 cut(s) 1367, 1573
PdmI GAANNNNTTC 1 cut(s) 1539
PfeI GAWTC 5 cut(s) 461, 848, 950, 1687, 1944
PkrI GCNGC 7 cut(s) 75, 1087, 1316, 1319, 1634, 1792, 2027
PleI GAGTC 3 cut(s) 847, 928, 1729
PpsI GAGTC 3 cut(s) 847, 928, 1729
Ppu21I YACGTR 1 cut(s) 2082
PpuMI RGGWCCY 1 cut(s) 1158
PsiI TTATAA 1 cut(s) 2088
Psp124BI GAGCTC 2 cut(s) 381, 1223
Psp1406I AACGTT 1 cut(s) 1821
Psp5II RGGWCCY 1 cut(s) 1158
PspFI CCCAGC 1 cut(s) 2127
PspN4I GGNNCC 2 cut(s) 1159, 1415
PspPI GGNCC 4 cut(s) 1022, 1158, 1172, 1304
PspPPI RGGWCCY 1 cut(s) 1158
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 1 cut(s) 2062
RsaI GTAC 9 cut(s) 87, 603, 739, 780, 861, 1290, 1401, 1415, 2136
RsaNI GTAC 9 cut(s) 86, 602, 738, 779, 860, 1289, 1400, 1414, 2135
SacI GAGCTC 2 cut(s) 381, 1223
SaqAI TTAA 7 cut(s) 278, 450, 695, 974, 1148, 1818, 1890
SatI GCNGC 7 cut(s) 74, 1086, 1315, 1318, 1633, 1791, 2026
Sau96I GGNCC 4 cut(s) 1022, 1158, 1172, 1304
SchI GAGTC 3 cut(s) 848, 929, 1730
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 3 cut(s) 381, 395, 1223
SfaNI GCATC 5 cut(s) 31, 106, 331, 996, 1022
SfcI CTRYAG 2 cut(s) 1098, 2175
Sfr274I CTCGAG 1 cut(s) 1425
SinI GGWCC 3 cut(s) 1022, 1158, 1172
SlaI CTCGAG 1 cut(s) 1425
SmiI ATTTAAAT 1 cut(s) 696
SmlI CTYRAG 2 cut(s) 1216, 1425
SmoI CTYRAG 2 cut(s) 1216, 1425
SphI GCATGC 1 cut(s) 435
SseBI AGGCCT 1 cut(s) 1348
SsiI CCGC 3 cut(s) 1035, 1085, 2139
SspMI CTAG 3 cut(s) 845, 1311, 1350
SstI GAGCTC 2 cut(s) 381, 1223
StuI AGGCCT 1 cut(s) 1348
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 4 cut(s) 396, 1442, 1994, 2113
SwaI ATTTAAAT 1 cut(s) 696
TaaI ACNGT 1 cut(s) 1714
TaiI ACGT 4 cut(s) 1550, 1824, 2084, 2201
TaqI TCGA 8 cut(s) 270, 375, 530, 1426, 1621, 1926, 2202, 2244
TatI WGTACW 3 cut(s) 85, 601, 859
TauI GCSGC 1 cut(s) 1088
TfiI GAWTC 5 cut(s) 461, 848, 950, 1687, 1944
Tru1I TTAA 7 cut(s) 278, 450, 695, 974, 1148, 1818, 1890
Tru9I TTAA 7 cut(s) 278, 450, 695, 974, 1148, 1818, 1890
TscAI CASTG 5 cut(s) 291, 630, 1053, 1717, 2037
TseFI GTSAC 3 cut(s) 840, 1599, 1708
TseI GCWGC 6 cut(s) 73, 1314, 1317, 1632, 1790, 2025
Tsp45I GTSAC 3 cut(s) 840, 1599, 1708
TspDTI ATGAA 9 cut(s) 474, 645, 765, 780, 1341, 1497, 1778, 2034, 2087
TspGWI ACGGA 1 cut(s) 1164
TspRI CASTG 5 cut(s) 291, 630, 1053, 1717, 2037
VpaK11BI GGWCC 3 cut(s) 1022, 1158, 1172
XapI RAATTY 8 cut(s) 254, 787, 929, 1166, 1394, 1698, 1810, 1970
XceI RCATGY 1 cut(s) 435
XcmI CCANNNNNNNNNTGG 1 cut(s) 2111
XhoI CTCGAG 1 cut(s) 1425
XmnI GAANNNNTTC 1 cut(s) 1539
XspI CTAG 3 cut(s) 845, 1311, 1350
ZraI GACGTC 1 cut(s) 1548
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.