MD06G1115400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000408
Physical Location & Seq
Forward (+)
25446096 .. 25457570
11475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1115400.v1.1.491

Sequence Viewer

Length: 3228 bp
ATGGCGCTGTCCTTAGTCGGAGGGGCGGCCATAGGAGCGCTGTTCGGAGCGTTGTATGATGTCGTTAAGATATCGATAGGCAAGACTGCTATGCAGTATAAACCCCTCCTCAGAGAACTTAAATTCACGTTAGACTCCCTAAGGCCGCGGATCATCCAACAGATAGGAGAACATAACGTGGAATTGGGTATTTCGAACGAAGATATAGTGAGCCTGCAAAGACAAATGGAGGAGGGTACAGAGCTCGTTAGGATGTTATCGAACCGTGGCACGTGGAGCTGCTACATCTTGTGCAGCTGCTGCAACTGCAATAAGCCGAGTTATGCGGAACAACTTGTTGAACTGGATAGGTCTCTTAGAATATTGTTGGAGATACTGAAGCTGCAGGAAGCGCGGGATGTGAAGGAGGTCTTGGTTTTGGCTAGGAAGATCAATGACAAACAAGATGAATTGGAAAGGAGACTGGCCGATTTGCTCAAAGTGCAGCAAGAATCGGGGGACGGGGGAGAGTCTTCGGGAAGTGGTGCAGAGACAACACCAAATGAACAAAATGGAGAGAATGGTGGACAACAAGTGGCACCGTCAGGTGGAGGAGTAGGGGCTGCTGCTCTAGGAGCGGCATTTGGGGTGTTGTTTGACACTGTTATGCAAGTGAAGGACAAGACTACCATGCTTAAACGCCCCCTCGGAGATCTCAAATCAACGCTAGATTCCGTAAAGCCGTTGATAGAAGAGGTGGCAGAATGTAGCAAGGTATTGAATCTCCCAAATGAGGAATTAGAAGATGTTAGAATACAAATGGAGAAGGGTGCAGAGCTTGTGAGCAATTGCTCAAAGATTCGTCACTGGACTACCTATAAAATGTATGAGTATACCAACAAACTTCTTGGATTGGACGAATCGCTTCAAAGATTGTTAACTGTGCTAAGATTGCAGTTAGCAAGGGATGTGAGGGAGACCCTGGTTTCCGTAAGCAATATAGAGGCGGTGATCAATCAAATTGAAGGGAGTGGTGTGGTTAAATTACAAAATGAGCAAAATGAAGCTAAAGGTTCATGTGAAGCGCCCGCAGCCATGGGTAAGAGTGCAGTTGGATTGAATGCACTAAGTGTGGAGGGAACAAGGGACGTGACTGAGACATTGGATCCGGAAGCGAATATAAAGGTAGGGTGGCAGAAAGTTGAAGAGAGTTGGGTGGCACGAAGTAAAACTGAACATCCATCACGTACAGTTGGACTGGATGCAATGAACATGCAGGTGGGAGCAAGGAGTATGGAGGAGACCTCTGTAAGTGATGTAGAGCCGGGGCTCAAGCGAATTCAGCGCAAAGAAGTATCCAAACTTGATGCGCCATTTAGGGTATTGGGGAAGGATGTTCTTAAGGATGAGGAAAATGAGGAACTTGATGAGCAAGTGGAAGATCATCATGACAGGAAGAAGAAAGGAGATGAGTCTGTGACCGGTGCAAGTGGAGATGGGATGGCGATTTGTAAAGCTGATGGGTGTGCAGCCGATCTGAGTGATGCAAAGGCGTATAATAGAAGGCATAGGGTGTGTGAACTTCATTCGAAGGCTCCACTTGTGATTGTGTCTGGTCTAAAGAGAAGGTTTTGCCATAATTGCAGCAGATTTCATGATCTATCAGAATTCGATGACACCAAACAAAGTTGTCGTAGGCATTTGCCCTACATTGAACGCGATGGTACAGGCACTCAGTTAAAGGACGTAAGTGGTGATATAGATACGGTGATCAGTCAAACTGAAAGGAGTGGTGCGGTACAATCACAAAACGACCAAAGTGAAATTAAAGGTTCATATGAAGTGGTGGAAGCCACACCACCAGCAGTTGGATTGAATGTACCGAGTGTGCAGGGAACAAGGGATTCAAGGGAAACGTTGCAGACCTCAGTGTGCAGGGAACAAGGGATTCATACAGAGCTAGGACTCAAGCAAATTCAGGGGAAAGATGTGTTTGATTCTGAACCTCCATACGCTGAATACAAGGAAACTGAATACGTACCTCCATCAGTTACAATTGACATGAATGTGCTTTTGAGAGGATTGAAGAGGTTTCTTCTTAATGAAGAGGTGTCAGCGGTTGTGCTAACGGGTCCTGGGGGAAGCGGGAAAACCACATTAGCAAAATTCTTCTGTGAAGATGAGGAAGTCAAAAATATATTCAAGAACAACGTCTTCTTTGTTCATGTTTCAGAAAATCCTAACTTGAGCCTTATGGTACAAACACTATATCAACAGATGGGTTTCCAGATACCTGCGTTGCAAAACGAATCAATTGCACTTCACTGGTTGCACCAATTTCTCATGGAAACAGCACTGCACCCATCAGTGTTGGTCTTGGATGATGTTTGGCCTGGATCAGAATCCCTTCTTGACAAGTTTGATGAATTCAAAACACCAAATTACAAGATTATGGTGACATCAAGGTTTGAATTTCCGGCATTTGGTTTTCCATATTATCTGAAACCACTGAATGCTGAAGATGAGATGACTCTTTCTCATCATTCCCGCGCACACAACATAGAATTACTGGATGATGAAGACCTGATATGTGTTTCTCCACCACCCATCACATTTGATGATGAAGACCCGATAACTCATTTTCCTGATTCCTCCGCATCCTACTTGGGAGACGGAAGCTCTCATGTTTCAACAGCTATAAGGCCAATGGATCAATTTCATGATTCCTCCGCATCAACCTTGGGAGATAAAAGCTCGTATGTTCCAAAAGATGCAGGGACAATGTTTGAGCCCGACAACCCCGTCAAGTCTAAGCAGACATATATGCAGCAGAAGTTGGAACTGAAGGTGAGTGCCAGTGACAAAGCAATGAAGAAAGTCATGAAAGCTGTCCACGCCATAAAGGGGGTTCATTCGACCAGTTGGGACGCGGAGAACCAGAGGCTAACAGTGATGGGGCAGTTGGATCCGGCGCTAATAGCTACTAGGTTGAGAAAGATTGCTTCGAAAACAGAGGTAATATCAGTTGGGCCAGCGAAGGAAGAGATCAAAGAAGACAAGAAAGATAAATTGACCGAGAGAAAAGGCGATAAGAAAGCTAGAAATCTCACTATGAAAGAGAAAATGGAAGACAAGAACCAAGAAAAGAAAGAGCGAAGTCTTACCGTGAAAGAGAAAATGGAGTTCGATAAAAGAGATGAGGAAGAGAGACTTCTAGATGAGAAAGAGAGAGTCATTACACCCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

1076

Amino Acids

119.75

Weight (kDa)

5.31

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 4 - 147 6.4e-16 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 199 - 328 1.2e-27 Arabidopsis broad-spectrum mildew resistance protein RPW8
SBP PF03110 497 - 561 1.4e-24 SBP domain
NB-ARC PF00931 687 - 815 4.8e-12 NB-ARC domain
AAA_22 PF13401 699 - 789 4.3e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1246
AasI GACNNNNNNGTC 1 cut(s) 2780
Acc36I ACCTGC 2 cut(s) 1246, 2281
AccB1I GGYRCC 1 cut(s) 577
AccB7I CCANNNNNTGG 1 cut(s) 1847
AccBSI CCGCTC 1 cut(s) 617
AccI GTMKAC 1 cut(s) 872
AccII CGCG 5 cut(s) 148, 394, 1698, 2529, 2909
AccIII TCCGGA 1 cut(s) 1147
AclI AACGTT 1 cut(s) 1895
AclWI GGATC 7 cut(s) 158, 1139, 1152, 2383, 2697, 2939, 2952
AcoI YGGCCR 2 cut(s) 27, 465
AcsI RAATTY 7 cut(s) 122, 1317, 1646, 1953, 2144, 2405, 2450
AcuI CTGAAG 3 cut(s) 398, 2517, 2843
AcvI CACGTG 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 7 cut(s) 238, 1228, 1705, 1779, 1860, 2019, 2238
AfeI AGCGCT 1 cut(s) 39
AfiI CCNNNNNNNGG 5 cut(s) 587, 772, 1847, 2462, 2883
AflII CTTAAG 1 cut(s) 1379
AgeI ACCGGT 1 cut(s) 1460
AjiI CACGTC 1 cut(s) 1129
AjnI CCWGG 4 cut(s) 960, 2113, 2371, 3221
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AleI CACNNNNGTG 1 cut(s) 3223
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 8 cut(s) 357, 454, 524, 950, 1130, 1274, 2643, 3181
AlwI GGATC 7 cut(s) 158, 1139, 1152, 2383, 2697, 2939, 2952
AlwNI CAGNNNCTG 1 cut(s) 300
Aor13HI TCCGGA 1 cut(s) 1147
Aor51HI AGCGCT 1 cut(s) 39
AoxI GGCC 6 cut(s) 27, 143, 465, 2369, 2681, 3008
ApoI RAATTY 7 cut(s) 122, 1317, 1646, 1953, 2144, 2405, 2450
AsiGI ACCGGT 1 cut(s) 1460
Asp700I GAANNNNTTC 2 cut(s) 903, 2385
AspLEI GCGC 8 cut(s) 7, 40, 394, 1066, 1326, 1351, 2531, 2953
AspS9I GGNCC 2 cut(s) 2111, 3008
AsuC2I CCSGG 1 cut(s) 1305
AsuHPI GGTGA 5 cut(s) 1000, 1745, 1759, 2446, 2839
AsuII TTCGAA 3 cut(s) 194, 1568, 2984
AvaII GGWCC 1 cut(s) 2111
AxyI CCTNAGG 1 cut(s) 140
BaeI ACNNNNGTAYC 2 cut(s) 2261, 2294
BamHI GGATCC 2 cut(s) 1144, 2944
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 3 cut(s) 246, 1311, 2772
BbrPI CACGTG 1 cut(s) 273
BbsI GAAGAC 6 cut(s) 504, 2185, 2565, 2610, 3039, 3114
Bbv12I GWGCWC 1 cut(s) 246
BceAI ACGGC 1 cut(s) 706
BcgI CGANNNNNNTGC 2 cut(s) 2276, 2310
BciT130I CCWGG 4 cut(s) 962, 2115, 2373, 3223
BciVI GTATCC 1 cut(s) 1345
BclI TGATCA 2 cut(s) 990, 1749
BcnI CCSGG 1 cut(s) 1305
BcoDI GTCTC 8 cut(s) 357, 454, 524, 950, 1130, 1274, 2643, 3181
BfaI CTAG 7 cut(s) 423, 611, 707, 1940, 2964, 3078, 3194
BfmI CTRYAG 1 cut(s) 383
BfoI RGCGCY 4 cut(s) 8, 41, 1067, 2954
BfrI CTTAAG 1 cut(s) 1379
BfuAI ACCTGC 2 cut(s) 1246, 2281
BfuI GTATCC 1 cut(s) 1345
BglII AGATCT 1 cut(s) 691
Bme1390I CCNGG 5 cut(s) 962, 1305, 2115, 2373, 3223
Bme18I GGWCC 1 cut(s) 2111
BmgBI CACGTC 1 cut(s) 1129
BmgT120I GGNCC 2 cut(s) 2111, 3008
BmiI GGNNCC 5 cut(s) 579, 1146, 1575, 2112, 2946
BmrFI CCNGG 5 cut(s) 962, 1305, 2115, 2373, 3223
BmsI GCATC 6 cut(s) 1231, 1336, 1513, 2645, 2720, 2740
BpiI GAAGAC 6 cut(s) 504, 2185, 2565, 2610, 3039, 3114
BplI GAGNNNNNCTC 2 cut(s) 1268, 1300
Bpu14I TTCGAA 3 cut(s) 194, 1568, 2984
BpuEI CTTGAG 3 cut(s) 1295, 1931, 2245
BpuMI CCSGG 1 cut(s) 1305
Bsa29I ATCGAT 1 cut(s) 74
BsaAI YACGTR 3 cut(s) 273, 1226, 2017
BsaBI GATNNNNATC 1 cut(s) 2380
BsaI GGTCTC 3 cut(s) 357, 950, 1274
BsaJI CCNNGG 9 cut(s) 146, 265, 685, 960, 1074, 1304, 2114, 2718, 3221
BsaWI WCCGGW 2 cut(s) 1147, 1460
Bsc4I CCNNNNNNNGG 5 cut(s) 587, 772, 1847, 2462, 2883
Bse118I RCCGGY 1 cut(s) 1460
Bse1I ACTGG 8 cut(s) 348, 468, 851, 1242, 2309, 2553, 2835, 2898
Bse21I CCTNAGG 1 cut(s) 140
Bse3DI GCAATG 2 cut(s) 1251, 2853
Bse8I GATNNNNATC 1 cut(s) 2380
BseAI TCCGGA 1 cut(s) 1147
BseBI CCWGG 4 cut(s) 962, 2115, 2373, 3223
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 9 cut(s) 146, 265, 685, 960, 1074, 1304, 2114, 2718, 3221
BseJI GATNNNNATC 1 cut(s) 2380
BseLI CCNNNNNNNGG 5 cut(s) 587, 772, 1847, 2462, 2883
BseMI GCAATG 2 cut(s) 1251, 2853
BseMII CTCAG 5 cut(s) 124, 1125, 1508, 1727, 1920
BseNI ACTGG 8 cut(s) 348, 468, 851, 1242, 2309, 2553, 2835, 2898
BseRI GAGGAG 4 cut(s) 98, 245, 606, 1292
BsgI GTGCAG 9 cut(s) 313, 503, 546, 831, 1107, 1527, 1889, 1933, 2321
Bsh1236I CGCG 5 cut(s) 148, 394, 1698, 2529, 2909
BshFI GGCC 6 cut(s) 29, 145, 467, 2371, 2683, 3010
BshNI GGYRCC 1 cut(s) 577
BshTI ACCGGT 1 cut(s) 1460
BshVI ATCGAT 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 5 cut(s) 1148, 1304, 1461, 2456, 2948
BslFI GGGAC 4 cut(s) 512, 1139, 2770, 2918
BslI CCNNNNNNNGG 5 cut(s) 587, 772, 1847, 2462, 2883
BsmAI GTCTC 8 cut(s) 357, 454, 524, 950, 1130, 1274, 2643, 3181
BsmBI CGTCTC 1 cut(s) 2643
BsmFI GGGAC 4 cut(s) 512, 1139, 2770, 2918
BsmI GAATGC 2 cut(s) 1105, 2497
BsnI GGCC 6 cut(s) 29, 145, 467, 2371, 2683, 3010
Bso31I GGTCTC 3 cut(s) 357, 950, 1274
Bsp119I TTCGAA 3 cut(s) 194, 1568, 2984
Bsp1286I GDGCHC 3 cut(s) 246, 1311, 2772
Bsp13I TCCGGA 1 cut(s) 1147
Bsp19I CCATGG 1 cut(s) 1074
BspANI GGCC 6 cut(s) 29, 145, 467, 2371, 2683, 3010
BspCNI CTCAG 5 cut(s) 123, 1126, 1509, 1726, 1919
BspDI ATCGAT 1 cut(s) 74
BspEI TCCGGA 1 cut(s) 1147
BspFNI CGCG 5 cut(s) 148, 394, 1698, 2529, 2909
BspHI TCATGA 4 cut(s) 1426, 1633, 2698, 2859
BspLI GGNNCC 5 cut(s) 579, 1146, 1575, 2112, 2946
BspMAI CTGCAG 1 cut(s) 387
BspMI ACCTGC 2 cut(s) 1246, 2281
BspPI GGATC 7 cut(s) 158, 1139, 1152, 2383, 2697, 2939, 2952
BspT104I TTCGAA 3 cut(s) 194, 1568, 2984
BspT107I GGYRCC 1 cut(s) 577
BspTI CTTAAG 1 cut(s) 1379
BspTNI GGTCTC 3 cut(s) 357, 950, 1274
BsrBI CCGCTC 1 cut(s) 617
BsrDI GCAATG 2 cut(s) 1251, 2853
BsrFI RCCGGY 1 cut(s) 1460
BsrI ACTGG 8 cut(s) 348, 468, 851, 1242, 2309, 2553, 2835, 2898
BssAI RCCGGY 1 cut(s) 1460
BssECI CCNNGG 9 cut(s) 146, 265, 685, 960, 1074, 1304, 2114, 2718, 3221
BssNAI GTATAC 1 cut(s) 873
BssT1I CCWWGG 2 cut(s) 1074, 2718
Bst1107I GTATAC 1 cut(s) 873
Bst2UI CCWGG 4 cut(s) 962, 2115, 2373, 3223
Bst4CI ACNGT 8 cut(s) 266, 582, 643, 922, 1231, 1747, 2929, 3145
Bst6I CTCTTC 6 cut(s) 726, 1179, 2060, 2079, 3015, 3177
BstAFI CTTAAG 1 cut(s) 1379
BstAPI GCANNNNNTGC 1 cut(s) 300
BstBAI YACGTR 3 cut(s) 273, 1226, 2017
BstBI TTCGAA 3 cut(s) 194, 1568, 2984
BstC8I GCNNGC 3 cut(s) 215, 1068, 3012
BstDSI CCRYGG 3 cut(s) 146, 265, 1074
BstFNI CGCG 5 cut(s) 148, 394, 1698, 2529, 2909
BstH2I RGCGCY 4 cut(s) 8, 41, 1067, 2954
BstHHI GCGC 8 cut(s) 7, 40, 394, 1066, 1326, 1351, 2531, 2953
BstMAI GTCTC 8 cut(s) 357, 454, 524, 950, 1130, 1274, 2643, 3181
BstNI CCWGG 4 cut(s) 962, 2115, 2373, 3223
BstNSI RCATGY 1 cut(s) 1255
BstSCI CCNGG 5 cut(s) 960, 1303, 2113, 2371, 3221
BstSFI CTRYAG 1 cut(s) 383
BstSNI TACGTA 1 cut(s) 2017
BstUI CGCG 5 cut(s) 148, 394, 1698, 2529, 2909
BstV2I GAAGAC 6 cut(s) 504, 2185, 2565, 2610, 3039, 3114
BstX2I RGATCY 3 cut(s) 691, 1144, 2944
BstYI RGATCY 3 cut(s) 691, 1144, 2944
BstZ17I GTATAC 1 cut(s) 873
Bsu15I ATCGAT 1 cut(s) 74
Bsu36I CCTNAGG 1 cut(s) 140
BsuI GTATCC 1 cut(s) 1345
BsuRI GGCC 6 cut(s) 29, 145, 467, 2371, 2683, 3010
BsuTUI ATCGAT 1 cut(s) 74
BtgI CCRYGG 3 cut(s) 146, 265, 1074
BtgZI GCGATG 1 cut(s) 1713
BtrI CACGTC 1 cut(s) 1129
BtsI GCAGTG 1 cut(s) 2333
BtsIMutI CAGTG 9 cut(s) 639, 844, 1914, 2302, 2333, 2352, 2486, 2842, 2934
BveI ACCTGC 2 cut(s) 1246, 2281
Cac8I GCNNGC 3 cut(s) 215, 1068, 3012
CaiI CAGNNNCTG 1 cut(s) 300
CciI TCATGA 4 cut(s) 1426, 1633, 2698, 2859
CfoI GCGC 8 cut(s) 7, 40, 394, 1066, 1326, 1351, 2531, 2953
Cfr10I RCCGGY 1 cut(s) 1460
Cfr13I GGNCC 2 cut(s) 2111, 3008
Cfr42I CCGCGG 1 cut(s) 149
ClaI ATCGAT 1 cut(s) 74
CseI GACGC 1 cut(s) 2915
Csp6I GTAC 7 cut(s) 237, 1227, 1704, 1778, 1859, 2018, 2237
CspAI ACCGGT 1 cut(s) 1460
CviQI GTAC 7 cut(s) 237, 1227, 1704, 1778, 1859, 2018, 2237
DraIII CACNNNGTG 1 cut(s) 1109
DrdI GACNNNNNNGTC 1 cut(s) 2780
DseDI GACNNNNNNGTC 1 cut(s) 2780
EaeI YGGCCR 2 cut(s) 27, 465
Eam1104I CTCTTC 6 cut(s) 726, 1179, 2060, 2079, 3015, 3177
EarI CTCTTC 6 cut(s) 726, 1179, 2060, 2079, 3015, 3177
Ecl136II GAGCTC 1 cut(s) 244
Eco105I TACGTA 1 cut(s) 2017
Eco130I CCWWGG 2 cut(s) 1074, 2718
Eco24I GRGCYC 3 cut(s) 246, 1311, 2772
Eco31I GGTCTC 3 cut(s) 357, 950, 1274
Eco32I GATATC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 2111
Eco47III AGCGCT 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 3 cut(s) 398, 2517, 2843
Eco72I CACGTG 1 cut(s) 273
Eco81I CCTNAGG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 2111
EcoRI GAATTC 3 cut(s) 1317, 1646, 2405
EcoRII CCWGG 4 cut(s) 960, 2113, 2371, 3221
EcoRV GATATC 1 cut(s) 72
EcoT14I CCWWGG 2 cut(s) 1074, 2718
EcoT38I GRGCYC 3 cut(s) 246, 1311, 2772
ErhI CCWWGG 2 cut(s) 1074, 2718
Esp3I CGTCTC 1 cut(s) 2643
FalI AAGNNNNNCTT 4 cut(s) 395, 427, 3174, 3206
FaqI GGGAC 4 cut(s) 512, 1139, 2770, 2918
FauI CCCGC 4 cut(s) 387, 1075, 2117, 2534
FauNDI CATATG 1 cut(s) 1816
FbaI TGATCA 2 cut(s) 990, 1749
FblI GTMKAC 1 cut(s) 872
FriOI GRGCYC 3 cut(s) 246, 1311, 2772
FspBI CTAG 7 cut(s) 423, 611, 707, 1940, 2964, 3078, 3194
GlaI GCGC 8 cut(s) 6, 39, 393, 1065, 1325, 1350, 2530, 2952
HaeII RGCGCY 4 cut(s) 8, 41, 1067, 2954
HaeIII GGCC 6 cut(s) 29, 145, 467, 2371, 2683, 3010
HapII CCGG 5 cut(s) 1148, 1304, 1461, 2456, 2948
HgaI GACGC 1 cut(s) 2915
HhaI GCGC 8 cut(s) 7, 40, 394, 1066, 1326, 1351, 2531, 2953
Hin6I GCGC 8 cut(s) 5, 38, 392, 1064, 1324, 1349, 2529, 2951
HinP1I GCGC 8 cut(s) 5, 38, 392, 1064, 1324, 1349, 2529, 2951
HincII GTYRAC 1 cut(s) 918
HindII GTYRAC 1 cut(s) 918
HpaI GTTAAC 1 cut(s) 918
HpaII CCGG 5 cut(s) 1148, 1304, 1461, 2456, 2948
HphI GGTGA 5 cut(s) 1000, 1745, 1759, 2446, 2839
Hpy166II GTNNAC 5 cut(s) 566, 873, 918, 1559, 2872
Hpy8I GTNNAC 5 cut(s) 566, 873, 918, 1559, 2872
HpyCH4III ACNGT 8 cut(s) 266, 582, 643, 922, 1231, 1747, 2929, 3145
HpyCH4IV ACGT 9 cut(s) 128, 177, 272, 1128, 1225, 1725, 1895, 2016, 2190
HpySE526I ACGT 9 cut(s) 128, 177, 272, 1128, 1225, 1725, 1895, 2016, 2190
HspAI GCGC 8 cut(s) 5, 38, 392, 1064, 1324, 1349, 2529, 2951
Kpn2I TCCGGA 1 cut(s) 1147
Ksp22I TGATCA 2 cut(s) 990, 1749
KspAI GTTAAC 1 cut(s) 918
KspI CCGCGG 1 cut(s) 149
LmnI GCTCC 6 cut(s) 35, 47, 276, 614, 1262, 1579
LweI GCATC 6 cut(s) 1231, 1336, 1513, 2645, 2720, 2740
MaeI CTAG 7 cut(s) 423, 611, 707, 1940, 2964, 3078, 3194
MaeII ACGT 9 cut(s) 128, 177, 272, 1128, 1225, 1725, 1895, 2016, 2190
MaeIII GTNAC 6 cut(s) 842, 1129, 1456, 2029, 2434, 2837
MbiI CCGCTC 1 cut(s) 617
MfeI CAATTG 3 cut(s) 826, 2034, 2292
MflI RGATCY 3 cut(s) 691, 1144, 2944
MhlI GDGCHC 3 cut(s) 246, 1311, 2772
MlyI GAGTC 6 cut(s) 128, 518, 1460, 1938, 2503, 3219
MmeI TCCRAC 7 cut(s) 181, 348, 1072, 1213, 1828, 2796, 2922
MroI TCCGGA 1 cut(s) 1147
MroXI GAANNNNTTC 2 cut(s) 903, 2385
MseI TTAA 9 cut(s) 66, 120, 675, 917, 1020, 1380, 1718, 1806, 2079
MslI CAYNNNNRTG 4 cut(s) 644, 1256, 2045, 3223
MspA1I CMGCKG 3 cut(s) 148, 297, 2096
MspCI CTTAAG 1 cut(s) 1379
MspI CCGG 5 cut(s) 1148, 1304, 1461, 2456, 2948
MspR9I CCNGG 5 cut(s) 962, 1305, 2115, 2373, 3223
MunI CAATTG 3 cut(s) 826, 2034, 2292
Mva1269I GAATGC 2 cut(s) 1105, 2497
MvaI CCWGG 4 cut(s) 962, 2115, 2373, 3223
MvnI CGCG 5 cut(s) 148, 394, 1698, 2529, 2909
NciI CCSGG 1 cut(s) 1305
NcoI CCATGG 1 cut(s) 1074
NdeI CATATG 1 cut(s) 1816
NlaIV GGNNCC 5 cut(s) 579, 1146, 1575, 2112, 2946
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 5 cut(s) 842, 1129, 1456, 2434, 2837
NspI RCATGY 1 cut(s) 1255
NspV TTCGAA 3 cut(s) 194, 1568, 2984
OliI CACNNNNGTG 1 cut(s) 3223
PagI TCATGA 4 cut(s) 1426, 1633, 2698, 2859
PaqCI CACCTGC 1 cut(s) 1246
PctI GAATGC 2 cut(s) 1105, 2497
PdmI GAANNNNTTC 2 cut(s) 903, 2385
PflMI CCANNNNNTGG 1 cut(s) 1847
PinAI ACCGGT 1 cut(s) 1460
PleI GAGTC 6 cut(s) 128, 517, 1459, 1938, 2503, 3218
PmaCI CACGTG 1 cut(s) 273
PmlI CACGTG 1 cut(s) 273
PpsI GAGTC 6 cut(s) 128, 517, 1459, 1938, 2503, 3218
Ppu21I YACGTR 3 cut(s) 273, 1226, 2017
PpuMI RGGWCCY 1 cut(s) 2111
Psp124BI GAGCTC 1 cut(s) 246
Psp1406I AACGTT 1 cut(s) 1895
Psp5II RGGWCCY 1 cut(s) 2111
Psp6I CCWGG 4 cut(s) 960, 2113, 2371, 3221
PspCI CACGTG 1 cut(s) 273
PspGI CCWGG 4 cut(s) 960, 2113, 2371, 3221
PspN4I GGNNCC 5 cut(s) 579, 1146, 1575, 2112, 2946
PspPI GGNCC 2 cut(s) 2111, 3008
PspPPI RGGWCCY 1 cut(s) 2111
PstI CTGCAG 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 300
PsuI RGATCY 3 cut(s) 691, 1144, 2944
PvuII CAGCTG 1 cut(s) 297
RsaI GTAC 7 cut(s) 238, 1228, 1705, 1779, 1860, 2019, 2238
RsaNI GTAC 7 cut(s) 237, 1227, 1704, 1778, 1859, 2018, 2237
RseI CAYNNNNRTG 4 cut(s) 644, 1256, 2045, 3223
SacI GAGCTC 1 cut(s) 246
SacII CCGCGG 1 cut(s) 149
SaqAI TTAA 9 cut(s) 66, 120, 675, 917, 1020, 1380, 1718, 1806, 2079
Sau96I GGNCC 2 cut(s) 2111, 3008
SchI GAGTC 6 cut(s) 128, 518, 1460, 1938, 2503, 3219
ScrFI CCNGG 5 cut(s) 962, 1305, 2115, 2373, 3223
SduI GDGCHC 3 cut(s) 246, 1311, 2772
SfaNI GCATC 6 cut(s) 1231, 1336, 1513, 2645, 2720, 2740
SfcI CTRYAG 1 cut(s) 383
Sfr303I CCGCGG 1 cut(s) 149
SfuI TTCGAA 3 cut(s) 194, 1568, 2984
SgrBI CCGCGG 1 cut(s) 149
SinI GGWCC 1 cut(s) 2111
SmiMI CAYNNNNRTG 4 cut(s) 644, 1256, 2045, 3223
SmlI CTYRAG 4 cut(s) 1310, 1379, 1946, 2224
SmoI CTYRAG 4 cut(s) 1310, 1379, 1946, 2224
SnaBI TACGTA 1 cut(s) 2017
SspI AATATT 1 cut(s) 363
SspMI CTAG 7 cut(s) 423, 611, 707, 1940, 2964, 3078, 3194
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 5 cut(s) 960, 1303, 2113, 2371, 3221
StyI CCWWGG 2 cut(s) 1074, 2718
TaaI ACNGT 8 cut(s) 266, 582, 643, 922, 1231, 1747, 2929, 3145
TaiI ACGT 9 cut(s) 131, 180, 275, 1131, 1228, 1728, 1898, 2019, 2193
TaqI TCGA 8 cut(s) 74, 194, 260, 1568, 1650, 2894, 2984, 3165
TaqII GACCGA 1 cut(s) 3068
TauI GCSGC 3 cut(s) 29, 148, 620
Tru1I TTAA 9 cut(s) 66, 120, 675, 917, 1020, 1380, 1718, 1806, 2079
Tru9I TTAA 9 cut(s) 66, 120, 675, 917, 1020, 1380, 1718, 1806, 2079
TscAI CASTG 9 cut(s) 646, 851, 1914, 2309, 2340, 2352, 2493, 2842, 2934
TseFI GTSAC 5 cut(s) 842, 1129, 1456, 2434, 2837
Tsp45I GTSAC 5 cut(s) 842, 1129, 1456, 2434, 2837
TspGWI ACGGA 3 cut(s) 703, 958, 2667
TspRI CASTG 9 cut(s) 646, 851, 1914, 2309, 2340, 2352, 2493, 2842, 2934
Van91I CCANNNNNTGG 1 cut(s) 1847
Vha464I CTTAAG 1 cut(s) 1379
VpaK11BI GGWCC 1 cut(s) 2111
XapI RAATTY 7 cut(s) 122, 1317, 1646, 1953, 2144, 2405, 2450
XbaI TCTAGA 1 cut(s) 3193
XceI RCATGY 1 cut(s) 1255
XmiI GTMKAC 1 cut(s) 872
XmnI GAANNNNTTC 2 cut(s) 903, 2385
XspI CTAG 7 cut(s) 423, 611, 707, 1940, 2964, 3078, 3194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.