RchiOBHm_Chr7g0186691

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000408
Physical Location & Seq
Forward (+)
6528565 .. 6530767
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16664

Sequence Viewer

Length: 2034 bp
ATGTCTATGAGTCTGAACTTTCTGGGAGGAGCACCTTTTCAACTCCTATACGATGGTGTTAGGGTAGCCATAGGTAGAACTACCAAGTTCAAAGCCCTCCTCTTAAATCTCGAATCGACGCTAGACTGTTTGCAACCACGAGTCATCCAGCAGATAAGAGACCATAATGTGGAGCTGAATCTCCCAAACGATATAATTCAAGACCTGCAAGTGAAAATGGATGATGGTGTGGCACTCATTGCCAATTTATCTAGGCTTAGCGTGTGGAACTGTCGCGTATGGGGGGACTGTTGCAACTGCATGAAGCCTAGCTATGCAGACCAACTTGCTGCATTGGATAGGTCTCTTAGACGACTGTTGGAGATACTAAAGCTTGAGCAGATGAGGAACGTGGTGGAGCTCTTGCTTTTGGCAAGGAGAAGTAATGACCGACAAGATGACTTGGAGAGAAGACAGTTGGAAATTCTGAAAGCGCTGCAAGAAACCGGGGATATGTTAAGAGCGCAGCAGGAAGTAATGGAGAGAATTGAAAGGAATGGTGCTGCATCTTCAGACAGAGGAGGGTCAGCTCCTGCTCCTGCTCCTGCTCTAGGAACAGTATTTAGGGTGTTGTTTGATGTTGTTATCAGAGTAAAGGTCAAGAATATGATGTATGAGCGAATCCTCAAAGATTTTGAATCCACTCTTGAATGCTTGAAGCCACTTATAGAAGAGATGGTGGAGTCTAACAAGTTGCTGCATCTTTCAAATGAGGAACAAGAATATTTCATAATGCAAATGGAAAAGGGTGTAGAGTTAATTCACCAGTGCTCTGAGGCTTGCAAGAGAGGAATAAGGTATAAAAAGTACGAATACACCAAGAAACTTCTGGGATTGGATGCATGTCTTCAAAGGCTTTTCATTGAACTCAAAGAGCAGGTAGTAAGGAATGTGAAGGAGGCCTTGGTGCTTATAAGTAACTTGGAAAAAGAGATCACAGAAATTGAAGGGGTTGTTGTGGTACAAAGTGATGAAATTGAGTGTTCGATTCCAGCACCTCAACCCCGATCACCTTTAGTTGGAGAGGATTTACAAAATGTGCAGATAAATCAAGAGGTGATCGAGGCTCTATGTCCCCCCCTTGTATTATGTGGTTTCATTAATCAAGGCTTGAGGGCAACTGCAACCGCACCCGCACCGGCCTTCGTTTTTATAGAAAATCAAGAGGTGATCGAGGAGACATTCGATGCAGTAAGCAACTTAGAAGAGGAGATCAAGGAAATTAGAGAAGTTCATGTGGTACAAAATAATGAAGTACAAAGTAAAGGTCCTCAACCTTCATTGCCTACAGCTGGATCCAGTGTACAAAGTATGCAGGGAACAAGGGATGTGATCAAGGAAAGATGGGACTCGGAAAAGTTGCCGGATGTCAAGCAAATTAAAGGGAAAGGCTTGTTCGAATATCCGGTTACTGGTGAATATGAACTTCCACCATTTCCAGTGGGACTTGCACAGGAAAGAGAAGATCACGCTGATGCATTCAGTAATGCATCATTGGAAGAACCGGTTGATGAATTGGATATGCATGTCAGCTACTTGAAACGGAAGCTTTTACATGGTGGTCACTTAGCCATTGTGCTGACGGGACCTGAAGGATGCGGGAAAACTAGATTGGCAGAAAAGTTTTGTCAAGATGAGGAAGTCATAGATGAATTCAAGAACAATATTTTCTTCGTCCCTGTTTCGGAAAGCCCCAAATTGCAGCTTATTGTTCAAAAACTATATCCAGAGGAGGGTTACAGGAAAATTATGTTGCCAGATGAAAATGGAGTTCATGCAATTCAGTGGCTGCAAGCATTTGTTAAGCATGTAGGACATCATCGTTCTCTGTTGGTCCTAGATGATGTCTGGCCTGGATCTGAATCCCTTCTTGATGAGTTTGATGAATTCAAAAGGTCAAATTTCAAGATTCTGGTGACATCAAGATTCGAATTTCCTAGATTTGGTTCCTCCTATTATGCAATGACGGATGATATACTTAGGGTAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

677

Amino Acids

76.96

Weight (kDa)

5.08

Isoelectric Point (pI)

59.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 137 5.5e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 195 - 325 5.6e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 522 - 655 1.3e-10 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
Acc36I ACCTGC 2 cut(s) 213, 907
AccB7I CCANNNNNTGG 1 cut(s) 169
AccII CGCG 1 cut(s) 276
AciI CCGC 3 cut(s) 1167, 1173, 1638
AclWI GGATC 3 cut(s) 1329, 1342, 1903
AcsI RAATTY 5 cut(s) 462, 1691, 1925, 1939, 1970
AcuI CTGAAG 2 cut(s) 534, 1650
AfaI GTAC 5 cut(s) 848, 1002, 1281, 1296, 1344
AfeI AGCGCT 1 cut(s) 474
AfiI CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1331, 1451, 1723, 1772, 1982
AgeI ACCGGT 1 cut(s) 1543
AjnI CCWGG 1 cut(s) 1891
AjuI GAANNNNNNNTTGG 4 cut(s) 926, 958, 1634, 1666
AluBI AGCT 9 cut(s) 175, 312, 373, 400, 569, 1331, 1572, 1588, 1744
AluI AGCT 9 cut(s) 175, 312, 373, 400, 569, 1331, 1572, 1588, 1744
Alw21I GWGCWC 3 cut(s) 34, 402, 812
Alw26I GTCTC 3 cut(s) 153, 348, 1211
AlwI GGATC 3 cut(s) 1329, 1342, 1903
AlwNI CAGNNNCTG 2 cut(s) 572, 1828
Aor51HI AGCGCT 1 cut(s) 474
AoxI GGCC 3 cut(s) 939, 1179, 1889
ApeKI GCWGC 7 cut(s) 329, 475, 505, 542, 736, 1741, 1828
ApoI RAATTY 5 cut(s) 462, 1691, 1925, 1939, 1970
ArsI GACNNNNNNTTYG 2 cut(s) 1291, 1323
AseI ATTAAT 1 cut(s) 1140
AsiGI ACCGGT 1 cut(s) 1543
Asp700I GAANNNNTTC 1 cut(s) 1905
AspLEI GCGC 2 cut(s) 475, 505
AspS9I GGNCC 3 cut(s) 1307, 1625, 1873
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 6 cut(s) 794, 1041, 1108, 1219, 1466, 1966
AsuII TTCGAA 2 cut(s) 1437, 1968
AvaII GGWCC 3 cut(s) 1307, 1625, 1873
BamHI GGATCC 1 cut(s) 1334
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 457, 878
Bbv12I GWGCWC 3 cut(s) 34, 402, 812
BbvI GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1753, 1815
BccI CCATC 4 cut(s) 47, 218, 709, 1377
BciT130I CCWGG 1 cut(s) 1893
BclI TGATCA 1 cut(s) 1371
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 3 cut(s) 153, 348, 1211
BfaI CTAG 7 cut(s) 122, 252, 309, 590, 1647, 1877, 1977
BfmI CTRYAG 1 cut(s) 1326
BfoI RGCGCY 1 cut(s) 476
BfuAI ACCTGC 2 cut(s) 213, 907
BisI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1742, 1829
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 7 cut(s) 331, 477, 507, 544, 738, 1743, 1830
Bme1390I CCNGG 2 cut(s) 487, 1893
Bme18I GGWCC 3 cut(s) 1307, 1625, 1873
BmgT120I GGNCC 3 cut(s) 1307, 1625, 1873
BmiI GGNNCC 3 cut(s) 1336, 1626, 1987
BmrFI CCNGG 2 cut(s) 487, 1893
BmsI GCATC 7 cut(s) 554, 748, 868, 1216, 1504, 1538, 1625
BpiI GAAGAC 2 cut(s) 457, 878
Bpu1102I GCTNAGC 1 cut(s) 257
Bpu14I TTCGAA 2 cut(s) 1437, 1968
BpuEI CTTGAG 2 cut(s) 395, 1171
BpuMI CCSGG 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 1900
BsaI GGTCTC 2 cut(s) 153, 348
BsaJI CCNNGG 2 cut(s) 486, 942
BsaWI WCCGGW 2 cut(s) 1444, 1543
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1331, 1451, 1723, 1772, 1982
Bse118I RCCGGY 2 cut(s) 1177, 1543
Bse1I ACTGG 4 cut(s) 805, 1338, 1456, 1478
Bse3DI GCAATG 3 cut(s) 237, 1319, 2007
Bse8I GATNNNNATC 1 cut(s) 1900
BseBI CCWGG 1 cut(s) 1893
BseDI CCNNGG 2 cut(s) 486, 942
BseGI GGATG 7 cut(s) 144, 226, 883, 1372, 1411, 1640, 2014
BseJI GATNNNNATC 1 cut(s) 1900
BseLI CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1331, 1451, 1723, 1772, 1982
BseMI GCAATG 3 cut(s) 237, 1319, 2007
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 4 cut(s) 805, 1338, 1456, 1478
BseRI GAGGAG 6 cut(s) 42, 89, 573, 1229, 1262, 1784
BseXI GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1753, 1815
BsgI GTGCAG 1 cut(s) 1100
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 3 cut(s) 941, 1181, 1891
BshTI ACCGGT 1 cut(s) 1543
BsiHKAI GWGCWC 3 cut(s) 34, 402, 812
BsiSI CCGG 5 cut(s) 486, 1178, 1403, 1445, 1544
BslFI GGGAC 6 cut(s) 299, 1098, 1400, 1497, 1638, 1700
BslI CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1331, 1451, 1723, 1772, 1982
BsmAI GTCTC 3 cut(s) 153, 348, 1211
BsmFI GGGAC 6 cut(s) 299, 1098, 1400, 1497, 1638, 1700
BsmI GAATGC 2 cut(s) 695, 1517
BsnI GGCC 3 cut(s) 941, 1181, 1891
Bso31I GGTCTC 2 cut(s) 153, 348
Bsp119I TTCGAA 2 cut(s) 1437, 1968
Bsp1286I GDGCHC 3 cut(s) 34, 402, 812
Bsp1407I TGTACA 1 cut(s) 1342
Bsp143I GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 3 cut(s) 1167, 1173, 1638
BspANI GGCC 3 cut(s) 941, 1181, 1891
BspCNI CTCAG 1 cut(s) 805
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 3 cut(s) 1336, 1626, 1987
BspMI ACCTGC 2 cut(s) 213, 907
BspPI GGATC 3 cut(s) 1329, 1342, 1903
BspT104I TTCGAA 2 cut(s) 1437, 1968
BspTNI GGTCTC 2 cut(s) 153, 348
BsrDI GCAATG 3 cut(s) 237, 1319, 2007
BsrFI RCCGGY 2 cut(s) 1177, 1543
BsrGI TGTACA 1 cut(s) 1342
BsrI ACTGG 4 cut(s) 805, 1338, 1456, 1478
BssAI RCCGGY 2 cut(s) 1177, 1543
BssECI CCNNGG 2 cut(s) 486, 942
BssMI GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 942
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 1893
Bst4CI ACNGT 6 cut(s) 128, 272, 290, 357, 456, 598
Bst6I CTCTTC 2 cut(s) 705, 1239
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 1342
BstBI TTCGAA 2 cut(s) 1437, 1968
BstC8I GCNNGC 2 cut(s) 820, 1833
BstDEI CTNAG 6 cut(s) 257, 347, 813, 1240, 1606, 2018
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 7 cut(s) 144, 226, 883, 1372, 1411, 1640, 2014
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 2 cut(s) 475, 505
BstKTI GATC 9 cut(s) 975, 1049, 1101, 1212, 1254, 1337, 1374, 1507, 1898
BstMAI GTCTC 3 cut(s) 153, 348, 1211
BstMBI GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 1893
BstNSI RCATGY 3 cut(s) 885, 1568, 1850
BstSCI CCNGG 2 cut(s) 485, 1891
BstSFI CTRYAG 1 cut(s) 1326
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1753, 1815
BstV2I GAAGAC 2 cut(s) 457, 878
BstX2I RGATCY 2 cut(s) 1334, 1895
BstYI RGATCY 2 cut(s) 1334, 1895
BsuRI GGCC 3 cut(s) 941, 1181, 1891
BtsCI GGATG 7 cut(s) 144, 226, 883, 1372, 1411, 1640, 2014
BtsIMutI CAGTG 4 cut(s) 812, 1345, 1485, 1829
BveI ACCTGC 2 cut(s) 213, 907
Cac8I GCNNGC 2 cut(s) 820, 1833
CaiI CAGNNNCTG 2 cut(s) 572, 1828
CfoI GCGC 2 cut(s) 475, 505
Cfr10I RCCGGY 2 cut(s) 1177, 1543
Cfr13I GGNCC 3 cut(s) 1307, 1625, 1873
CseI GACGC 1 cut(s) 127
Csp6I GTAC 5 cut(s) 847, 1001, 1280, 1295, 1343
CspAI ACCGGT 1 cut(s) 1543
CviAII CATG 7 cut(s) 301, 882, 1274, 1565, 1595, 1814, 1847
CviQI GTAC 5 cut(s) 847, 1001, 1280, 1295, 1343
DdeI CTNAG 6 cut(s) 257, 347, 813, 1240, 1606, 2018
DpnI GATC 9 cut(s) 974, 1048, 1100, 1211, 1253, 1336, 1373, 1506, 1897
DpnII GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
Eam1104I CTCTTC 2 cut(s) 705, 1239
EarI CTCTTC 2 cut(s) 705, 1239
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 942
Eco147I AGGCCT 1 cut(s) 941
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 2 cut(s) 153, 348
Eco47I GGWCC 3 cut(s) 1307, 1625, 1873
Eco47III AGCGCT 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 534, 1650
EcoICRI GAGCTC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoO109I RGGNCCY 2 cut(s) 1307, 1625
EcoRI GAATTC 2 cut(s) 1691, 1925
EcoRII CCWGG 1 cut(s) 1891
EcoT14I CCWWGG 1 cut(s) 942
EcoT22I ATGCAT 4 cut(s) 883, 1519, 1531, 1566
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 942
FaeI CATG 7 cut(s) 304, 885, 1277, 1568, 1598, 1817, 1850
FalI AAGNNNNNCTT 2 cut(s) 926, 958
FaqI GGGAC 6 cut(s) 299, 1098, 1400, 1497, 1638, 1700
FatI CATG 7 cut(s) 300, 881, 1273, 1564, 1594, 1813, 1846
FauI CCCGC 2 cut(s) 1180, 1631
FbaI TGATCA 1 cut(s) 1371
Fnu4HI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1742, 1829
FokI GGATG 7 cut(s) 131, 233, 890, 1379, 1418, 1647, 2021
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1742, 1829
FspBI CTAG 7 cut(s) 122, 252, 309, 590, 1647, 1877, 1977
GlaI GCGC 2 cut(s) 474, 504
GluI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1742, 1829
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 3 cut(s) 941, 1181, 1891
HapII CCGG 5 cut(s) 486, 1178, 1403, 1445, 1544
HgaI GACGC 1 cut(s) 127
HhaI GCGC 2 cut(s) 475, 505
Hin1II CATG 7 cut(s) 304, 885, 1277, 1568, 1598, 1817, 1850
Hin6I GCGC 2 cut(s) 473, 503
HinP1I GCGC 2 cut(s) 473, 503
HindIII AAGCTT 2 cut(s) 371, 1586
HpaII CCGG 5 cut(s) 486, 1178, 1403, 1445, 1544
HphI GGTGA 6 cut(s) 794, 1041, 1108, 1219, 1466, 1966
Hpy166II GTNNAC 1 cut(s) 1343
Hpy188I TCNGA 8 cut(s) 15, 468, 553, 629, 814, 1393, 1726, 1900
Hpy8I GTNNAC 1 cut(s) 1343
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 6 cut(s) 928, 980, 1192, 1326, 1625, 1916
HpyCH4III ACNGT 6 cut(s) 128, 272, 290, 357, 456, 598
HpyCH4IV ACGT 1 cut(s) 390
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 6 cut(s) 257, 347, 813, 1240, 1606, 2018
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 7 cut(s) 304, 885, 1277, 1568, 1598, 1817, 1850
HspAI GCGC 2 cut(s) 473, 503
Ksp22I TGATCA 1 cut(s) 1371
Kzo9I GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
LmnI GCTCC 6 cut(s) 29, 172, 397, 574, 580, 586
Lsp1109I GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1753, 1815
LweI GCATC 7 cut(s) 554, 748, 868, 1216, 1504, 1538, 1625
MaeI CTAG 7 cut(s) 122, 252, 309, 590, 1647, 1877, 1977
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 5 cut(s) 956, 1447, 1601, 1775, 1954
MalI GATC 9 cut(s) 974, 1048, 1100, 1211, 1253, 1336, 1373, 1506, 1897
MboI GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
MboII GAAGA 8 cut(s) 462, 540, 722, 878, 1256, 1514, 1550, 1702
MflI RGATCY 2 cut(s) 1334, 1895
MhlI GDGCHC 3 cut(s) 34, 402, 812
MlyI GAGTC 4 cut(s) 19, 150, 731, 1382
MmeI TCCRAC 3 cut(s) 339, 438, 1039
Mph1103I ATGCAT 4 cut(s) 883, 1519, 1531, 1566
MroXI GAANNNNTTC 1 cut(s) 1905
MseI TTAA 6 cut(s) 104, 497, 797, 1140, 1419, 1842
MslI CAYNNNNRTG 1 cut(s) 1512
MspA1I CMGCKG 1 cut(s) 1331
MspI CCGG 5 cut(s) 486, 1178, 1403, 1445, 1544
MspR9I CCNGG 2 cut(s) 487, 1893
Mva1269I GAATGC 2 cut(s) 695, 1517
MvaI CCWGG 1 cut(s) 1893
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 487
NdeII GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
NlaIII CATG 7 cut(s) 304, 885, 1277, 1568, 1598, 1817, 1850
NlaIV GGNNCC 3 cut(s) 1336, 1626, 1987
NmuCI GTSAC 2 cut(s) 1601, 1954
NsiI ATGCAT 4 cut(s) 883, 1519, 1531, 1566
NspI RCATGY 3 cut(s) 885, 1568, 1850
NspV TTCGAA 2 cut(s) 1437, 1968
PceI AGGCCT 1 cut(s) 941
PctI GAATGC 2 cut(s) 695, 1517
PdmI GAANNNNTTC 1 cut(s) 1905
PfeI GAWTC 8 cut(s) 113, 178, 660, 677, 1027, 1901, 1948, 1965
PflMI CCANNNNNTGG 1 cut(s) 169
PinAI ACCGGT 1 cut(s) 1543
PkrI GCNGC 7 cut(s) 331, 477, 507, 544, 738, 1743, 1830
PleI GAGTC 4 cut(s) 18, 149, 730, 1382
PpsI GAGTC 4 cut(s) 18, 149, 730, 1382
PpuMI RGGWCCY 2 cut(s) 1307, 1625
PshBI ATTAAT 1 cut(s) 1140
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 2 cut(s) 1307, 1625
Psp6I CCWGG 1 cut(s) 1891
PspGI CCWGG 1 cut(s) 1891
PspN4I GGNNCC 3 cut(s) 1336, 1626, 1987
PspPI GGNCC 3 cut(s) 1307, 1625, 1873
PspPPI RGGWCCY 2 cut(s) 1307, 1625
PstNI CAGNNNCTG 2 cut(s) 572, 1828
PsuI RGATCY 2 cut(s) 1334, 1895
PvuII CAGCTG 1 cut(s) 1331
RsaI GTAC 5 cut(s) 848, 1002, 1281, 1296, 1344
RsaNI GTAC 5 cut(s) 847, 1001, 1280, 1295, 1343
RseI CAYNNNNRTG 1 cut(s) 1512
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 6 cut(s) 104, 497, 797, 1140, 1419, 1842
SatI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1742, 1829
Sau3AI GATC 9 cut(s) 972, 1046, 1098, 1209, 1251, 1334, 1371, 1504, 1895
Sau96I GGNCC 3 cut(s) 1307, 1625, 1873
SchI GAGTC 4 cut(s) 19, 150, 731, 1382
ScrFI CCNGG 2 cut(s) 487, 1893
SduI GDGCHC 3 cut(s) 34, 402, 812
SfaNI GCATC 7 cut(s) 554, 748, 868, 1216, 1504, 1538, 1625
SfcI CTRYAG 1 cut(s) 1326
SfuI TTCGAA 2 cut(s) 1437, 1968
SinI GGWCC 3 cut(s) 1307, 1625, 1873
SmiMI CAYNNNNRTG 1 cut(s) 1512
SmlI CTYRAG 2 cut(s) 374, 1150
SmoI CTYRAG 2 cut(s) 374, 1150
SseBI AGGCCT 1 cut(s) 941
SsiI CCGC 3 cut(s) 1167, 1173, 1638
SspI AATATT 2 cut(s) 764, 1705
SspMI CTAG 7 cut(s) 122, 252, 309, 590, 1647, 1877, 1977
SstI GAGCTC 1 cut(s) 402
StuI AGGCCT 1 cut(s) 941
StyD4I CCNGG 2 cut(s) 485, 1891
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 6 cut(s) 128, 272, 290, 357, 456, 598
TaiI ACGT 1 cut(s) 393
TaqI TCGA 8 cut(s) 111, 116, 1025, 1101, 1212, 1224, 1437, 1968
TaqII GACCGA 1 cut(s) 444
TatI WGTACW 2 cut(s) 1294, 1342
TfiI GAWTC 8 cut(s) 113, 178, 660, 677, 1027, 1901, 1948, 1965
Tru1I TTAA 6 cut(s) 104, 497, 797, 1140, 1419, 1842
Tru9I TTAA 6 cut(s) 104, 497, 797, 1140, 1419, 1842
TscAI CASTG 4 cut(s) 812, 1345, 1485, 1829
TseFI GTSAC 2 cut(s) 1601, 1954
TseI GCWGC 7 cut(s) 329, 475, 505, 542, 736, 1741, 1828
Tsp45I GTSAC 2 cut(s) 1601, 1954
TspGWI ACGGA 2 cut(s) 1597, 2021
TspRI CASTG 4 cut(s) 812, 1345, 1485, 1829
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 3 cut(s) 1307, 1625, 1873
VspI ATTAAT 1 cut(s) 1140
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 5 cut(s) 462, 1691, 1925, 1939, 1970
XceI RCATGY 3 cut(s) 885, 1568, 1850
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XmnI GAANNNNTTC 1 cut(s) 1905
XspI CTAG 7 cut(s) 122, 252, 309, 590, 1647, 1877, 1977
Zsp2I ATGCAT 4 cut(s) 883, 1519, 1531, 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.