Rh2DG179300

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000408
Physical Location & Seq
Forward (+)
15802602 .. 15807292
4691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG179300.1

Sequence Viewer

Length: 2622 bp
ATGGCAGTGGAGTTACTTGGAGGAGCAGTGTTTACACTGCTGTATGATGGGGTTAAGCAAGCAATCGGAAAGACAGGGACTTTCAAATACCTTCTCAAAGACATCAAATCCACACTGGAGTCTTTGAACAAGCTACACGCCACGGCCATCAGACAAATCGGAGAGTATAACCTGGAATTGGGTCTCCCAAATGATGATATAGAAGAGCTCAAAGAGCAAATGGAGGAAGGGGCAAAGCTGGTGGGAAAGTTGTCTGATTTTCGTGTGTGGAACTACTGCTGCTTAAGTTGTTATACCGATCAACTTGTTGATTTGGATAGGTCTCTCAAGAGGCTGTTGGAGAATCTTAAGTTGCAAGAAACTAGAGATGTGAAAGAGATCTTGGATTTGCTGAGGAAATACCGCGTTGAGCTTGATGAGTTGAAGGGAACGTTGCAGATATTACTGCAGCAGAGAGCAGGAGATGTGAAGGAGACCACCTCGAATTCAACTGCAGGAAGCAGTACAGAGACCATGCCTCAGCAAAATGTAGGGAACGGAGAGGAACAAGAAGTGAAGGACAAGACTTTGATGTTCAGACCCCTACTCGGAGATCTCAAGTCCATGCTAGATTCCTTAGAACCACTGATCAAAGAGATAGATCAATACAATAGCGTATTGGTTCTCCCAAAGCCGGAGGTAGAAAAGTTCAGGACACAAATGGACAAGGGTGTGGAGCTCGTTTGCAAGTGCTCCAAGGTAGCTGGGGAGGTGAGTGAGACATTGGCTTCAATAAGTAACCTAAAGGAGGTAATCAAGCAATATGAAGGGCCTAGTGTCATACAAAATCAACATGAGATTATAGGTTCCTGCAAAGTACCTGAACCTTCATCGCCTACATTGATGCCTGAGATTGAGGGTTCTGATGAAGTGCATCCATTACCAGTGCCTCCTGGAGCAAAACTACCTTCACCAAAGGAGGAAGTTGGGGAAGTGAGCGAGACATTGGCTTCAATAAGTAACCTAAAGGAGGTAGTCAAGCAATATGAAGGGCCTAGTGTCATACAAAATCAACATGAGATTATAGGTTCCTGCAAAGTACCCGAACCTTCATCGCCTACAGTGATGCCTGAGATTGAGGATTCTGTTGAAGTGCATCCATTACCAGTGCCTCCTGGAGCAAAACTACCTTCACCAAAGGTTCTAATTACATCTGAACCGGAGTCCCAGCCAATGAAAGGTCAATGGCAAAAGGGAAATCTTATTGGTCGTGGCACGTTTGGGAATGAAATCAAAGTCCTCAGCCGGCTCAAGCATCCTAATATTGTGCAATATTATGGGACCGAGATAGTTGGAGATAGATTTTTTATATATCAGGAGTATGTCCATCCTGTATCTATTAACAGATACATCCAAGAATATTGTGGAGCCATGACAGAAGCTGTTGTTCGCAGTTTAACTCACCAGATAGTGTCTGGGTTGGCTTACTTGCACAGCAAAGAAACGATACACGGGGACATCAAAGGGGATAATTTGCTCGTTGAGTCATCTGGTGTTGTCAAGATAGCTGACTTTGGAATTGTAAAACATCTTCAAGGAAGACTCCTAAAGGGAGGTCCATACTGGATGGCTCCAGAGATCATCAAAAATGGGATACCGAACGATAAATGCTCTGATCATGATTTGGCTCGTGGTGGTGACATATGGAGTTTGGGTTGCACTGTTATTGAATTGTTCACTGGAAAACCTCCCTGGAGTGAGTTCGAACAGCTTCAAGCCATGTTTCTAATTGTTCGTAAAGATCCTCCAATTCCAGAAACATTGTCACCTGAGGGTAAGGATTTCCTAACATGCTGCTTGCAGAGAAACCCTGCAGAGAGGCCAACTGCTGCAATGCTTTTAGAACATCCATTCTTAAAGAACTCCCAGCCGCAGGATGTCCCAACTATGACCGGTGCCATTAATCTGGTGAATTTGATGGATAAACACAAAATGACGGAACTCCAATCGTACTTGAAAGAAGACTTGATCGTTGAGATTGAGAAGATCAGGTTCTCCACTGATCCATCAATCCATCACCGCGGGATGTCTCTCGAAAGAGGCAAGGAATTCTCAGGAGGACAAGGATCGTCATTGGGGTACTCGAGTCCGAGTCCCACCGATCATCATGACCAGCAGCAGCAGCCAGTGACGCTCAGCAGGCATGAGATGCAGAAGAGGAGGGACTGGAACACTTTCGGGCAGTACTTGAAGAACCAGAGGCCCCCAGTGCCTCCCTCCCAGTGCAATTACAGCCATGTTCTCGAGTTCCTCAGGTACCTCGACCAGTTCGGGAAGACCAAAGTCCATCTCCAGGGTTGTGTGTTTTACGGCCAGCCGGAGCCACCTGCGCCTTGCACCTGTCCTCTCCGGCAGGCCTGGGGAAGCCTGGATGCTCTCATAGGGAGGCTGAGGGCTGCTTATGAGGAGAACGGTGGGGCCCCTGAGAGCAATCCTTTTGCTAGTGGTGCAATCAGGGGGTACTTGAGGGAGGTCAGGGAGTGTCAGGCCAAGGCTAGAGGGATTCCTTACAATAATAAGAAGAAGAAGCCTGGAAATAGTGATCGTGATTCTTCAACAAGTTCTATCATGCACTTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

873

Amino Acids

97.39

Weight (kDa)

5.85

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 140 9.4e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 184 - 247 9.2e-10 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 419 - 632 6.7e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 419 - 628 2e-35 Protein tyrosine and serine/threonine kinase
ALOG_dom PF04852 717 - 840 2.5e-62 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2374
Acc36I ACCTGC 1 cut(s) 2374
Acc65I GGTACC 1 cut(s) 2295
AccB1I GGYRCC 2 cut(s) 1934, 2295
AccII CGCG 2 cut(s) 405, 2061
AciI CCGC 4 cut(s) 403, 1910, 2059, 2061
AclI AACGTT 1 cut(s) 431
AclWI GGATC 3 cut(s) 1775, 2036, 2113
AcoI YGGCCR 2 cut(s) 144, 2350
AcsI RAATTY 3 cut(s) 484, 1951, 2087
AfaI GTAC 8 cut(s) 505, 858, 1080, 1991, 2120, 2225, 2297, 2501
AfiI CCNNNNNNNGG 8 cut(s) 178, 587, 673, 787, 1009, 1217, 1912, 2332
AflII CTTAAG 2 cut(s) 283, 347
AgeI ACCGGT 1 cut(s) 1931
AjnI CCWGG 8 cut(s) 171, 931, 1153, 1730, 2331, 2396, 2406, 2569
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 9 cut(s) 133, 208, 238, 412, 718, 743, 1421, 1547, 1750
AluI AGCT 9 cut(s) 133, 208, 238, 412, 718, 743, 1421, 1547, 1750
Alw21I GWGCWC 3 cut(s) 210, 720, 734
Alw26I GTCTC 7 cut(s) 188, 327, 467, 503, 752, 974, 2073
AlwI GGATC 3 cut(s) 1775, 2036, 2113
AlwNI CAGNNNCTG 1 cut(s) 1421
Ama87I CYCGRG 2 cut(s) 2122, 2282
AoxI GGCC 9 cut(s) 144, 809, 1031, 1859, 2240, 2350, 2394, 2457, 2526
ApaI GGGCCC 1 cut(s) 2461
ApeKI GCWGC 8 cut(s) 279, 448, 1833, 1868, 2155, 2158, 2161, 2435
ApoI RAATTY 3 cut(s) 484, 1951, 2087
AseI ATTAAT 1 cut(s) 1941
AsiGI ACCGGT 1 cut(s) 1931
Asp700I GAANNNNTTC 2 cut(s) 1749, 2213
Asp718I GGTACC 1 cut(s) 2295
AspLEI GCGC 1 cut(s) 2371
AspS9I GGNCC 7 cut(s) 809, 1031, 1320, 1595, 2241, 2457, 2458
AsuHPI GGTGA 8 cut(s) 763, 942, 1164, 1433, 1688, 1797, 1960, 2048
AsuII TTCGAA 1 cut(s) 1743
AvaI CYCGRG 2 cut(s) 2122, 2282
AvaII GGWCC 2 cut(s) 1320, 1595
AxyI CCTNAGG 2 cut(s) 1809, 2291
BaeGI GKGCMC 1 cut(s) 2461
BaeI ACNNNNGTAYC 2 cut(s) 2110, 2143
BanI GGYRCC 2 cut(s) 1934, 2295
BanII GRGCYC 3 cut(s) 210, 720, 2461
BarI GAAGNNNNNNTAC 2 cut(s) 1554, 1586
BauI CACGAG 1 cut(s) 1668
BbsI GAAGAC 3 cut(s) 1585, 2007, 2321
Bbv12I GWGCWC 3 cut(s) 210, 720, 734
BbvCI CCTCAGC 4 cut(s) 392, 519, 1280, 2429
BbvI GCAGC 8 cut(s) 266, 460, 1820, 1855, 2167, 2170, 2173, 2422
BccI CCATC 8 cut(s) 41, 155, 1374, 1600, 1951, 2053, 2061, 2334
BceAI ACGGC 2 cut(s) 159, 2365
BciT130I CCWGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
BciVI GTATCC 1 cut(s) 1626
BclI TGATCA 2 cut(s) 627, 1654
BcoDI GTCTC 7 cut(s) 188, 327, 467, 503, 752, 974, 2073
BfaI CTAG 6 cut(s) 363, 608, 813, 1035, 2481, 2535
BfmI CTRYAG 4 cut(s) 446, 492, 1098, 1851
BfrI CTTAAG 2 cut(s) 283, 347
BfuAI ACCTGC 1 cut(s) 2374
BfuI GTATCC 1 cut(s) 1626
BglII AGATCT 2 cut(s) 378, 592
BisI GCNGC 9 cut(s) 280, 449, 1834, 1869, 1910, 2156, 2159, 2162, 2436
BlpI GCTNAGC 1 cut(s) 2174
BlsI GCNGC 9 cut(s) 281, 450, 1835, 1870, 1911, 2157, 2160, 2163, 2437
BmcAI AGTACT 1 cut(s) 2225
Bme1390I CCNGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
Bme18I GGWCC 2 cut(s) 1320, 1595
BmeT110I CYCGRG 2 cut(s) 2122, 2282
BmgT120I GGNCC 7 cut(s) 809, 1031, 1320, 1595, 2241, 2457, 2458
BmrFI CCNGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
BmrI ACTGGG 2 cut(s) 2240, 2254
BmsI GCATC 7 cut(s) 873, 922, 1095, 1144, 1303, 2178, 2401
BmuI ACTGGG 2 cut(s) 2240, 2254
BoxI GACNNNNGTC 1 cut(s) 2321
BpiI GAAGAC 3 cut(s) 1585, 2007, 2321
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 6 cut(s) 137, 954, 1176, 1596, 1753, 2315
Bpu10I CCTNAGC 4 cut(s) 392, 519, 1280, 2429
Bpu1102I GCTNAGC 1 cut(s) 2174
Bpu14I TTCGAA 1 cut(s) 1743
BpuEI CTTGAG 4 cut(s) 311, 581, 1274, 2524
BsaI GGTCTC 4 cut(s) 188, 327, 467, 503
BsaJI CCNNGG 7 cut(s) 141, 735, 1730, 2059, 2332, 2397, 2529
BsaWI WCCGGW 2 cut(s) 1198, 1931
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 1678, 1708
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 587, 673, 787, 1009, 1217, 1912, 2332
Bse118I RCCGGY 2 cut(s) 1284, 1931
Bse21I CCTNAGG 2 cut(s) 1809, 2291
Bse3DI GCAATG 1 cut(s) 1878
BseBI CCWGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
BseDI CCNNGG 7 cut(s) 141, 735, 1730, 2059, 2332, 2397, 2529
BseLI CCNNNNNNNGG 8 cut(s) 178, 587, 673, 787, 1009, 1217, 1912, 2332
BseMI GCAATG 1 cut(s) 1878
BseRI GAGGAG 3 cut(s) 36, 2212, 2459
BseSI GKGCMC 1 cut(s) 2461
BseXI GCAGC 8 cut(s) 266, 460, 1820, 1855, 2167, 2170, 2173, 2422
BseYI CCCAGC 3 cut(s) 743, 1206, 1905
Bsh1236I CGCG 2 cut(s) 405, 2061
BshFI GGCC 9 cut(s) 146, 811, 1033, 1861, 2242, 2352, 2396, 2459, 2528
BshNI GGYRCC 2 cut(s) 1934, 2295
BshTI ACCGGT 1 cut(s) 1931
BsiHKAI GWGCWC 3 cut(s) 210, 720, 734
BsiHKCI CYCGRG 2 cut(s) 2122, 2282
BsiSI CCGG 6 cut(s) 674, 1199, 1285, 1932, 2357, 2389
BslFI GGGAC 7 cut(s) 91, 1189, 1333, 1508, 1904, 2118, 2216
BslI CCNNNNNNNGG 8 cut(s) 178, 587, 673, 787, 1009, 1217, 1912, 2332
BsmAI GTCTC 7 cut(s) 188, 327, 467, 503, 752, 974, 2073
BsmFI GGGAC 7 cut(s) 91, 1189, 1333, 1508, 1904, 2118, 2216
BsnI GGCC 9 cut(s) 146, 811, 1033, 1861, 2242, 2352, 2396, 2459, 2528
Bso31I GGTCTC 4 cut(s) 188, 327, 467, 503
BsoBI CYCGRG 2 cut(s) 2122, 2282
Bsp119I TTCGAA 1 cut(s) 1743
Bsp120I GGGCCC 1 cut(s) 2457
Bsp1286I GDGCHC 4 cut(s) 210, 720, 734, 2461
Bsp1720I GCTNAGC 1 cut(s) 2174
BspACI CCGC 4 cut(s) 403, 1910, 2059, 2061
BspANI GGCC 9 cut(s) 146, 811, 1033, 1861, 2242, 2352, 2396, 2459, 2528
BspFNI CGCG 2 cut(s) 405, 2061
BspHI TCATGA 2 cut(s) 1657, 2146
BspMAI CTGCAG 3 cut(s) 450, 496, 1855
BspMI ACCTGC 1 cut(s) 2374
BspPI GGATC 3 cut(s) 1775, 2036, 2113
BspQI GCTCTTC 1 cut(s) 198
BspT104I TTCGAA 1 cut(s) 1743
BspT107I GGYRCC 2 cut(s) 1934, 2295
BspTI CTTAAG 2 cut(s) 283, 347
BspTNI GGTCTC 4 cut(s) 188, 327, 467, 503
BsrDI GCAATG 1 cut(s) 1878
BsrFI RCCGGY 2 cut(s) 1284, 1931
BssAI RCCGGY 2 cut(s) 1284, 1931
BssECI CCNNGG 7 cut(s) 141, 735, 1730, 2059, 2332, 2397, 2529
BssSI CACGAG 1 cut(s) 1668
BssT1I CCWWGG 2 cut(s) 735, 2529
Bst2BI CACGAG 1 cut(s) 1668
Bst2UI CCWGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
Bst4CI ACNGT 3 cut(s) 1102, 1702, 2453
Bst6I CTCTTC 2 cut(s) 198, 2189
BstAFI CTTAAG 2 cut(s) 283, 347
BstAPI GCANNNNNTGC 1 cut(s) 2188
BstBI TTCGAA 1 cut(s) 1743
BstC8I GCNNGC 6 cut(s) 60, 1286, 1838, 2180, 2354, 2394
BstDSI CCRYGG 2 cut(s) 141, 2059
BstENI CCTNNNNNAGG 2 cut(s) 785, 1007
BstFNI CGCG 2 cut(s) 405, 2061
BstHHI GCGC 1 cut(s) 2371
BstMAI GTCTC 7 cut(s) 188, 327, 467, 503, 752, 974, 2073
BstMWI GCNNNNNNNGC 9 cut(s) 214, 2161, 2170, 2179, 2188, 2248, 2271, 2368, 2486
BstNI CCWGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
BstNSI RCATGY 1 cut(s) 1833
BstPAI GACNNNNGTC 1 cut(s) 2321
BstSCI CCNGG 8 cut(s) 171, 931, 1153, 1730, 2331, 2396, 2406, 2569
BstSFI CTRYAG 4 cut(s) 446, 492, 1098, 1851
BstSLI GKGCMC 1 cut(s) 2461
BstUI CGCG 2 cut(s) 405, 2061
BstV1I GCAGC 8 cut(s) 266, 460, 1820, 1855, 2167, 2170, 2173, 2422
BstV2I GAAGAC 3 cut(s) 1585, 2007, 2321
BstX2I RGATCY 3 cut(s) 378, 592, 1780
BstXI CCANNNNNNTGG 1 cut(s) 1945
BstYI RGATCY 3 cut(s) 378, 592, 1780
Bsu36I CCTNAGG 2 cut(s) 1809, 2291
BsuI GTATCC 1 cut(s) 1626
BsuRI GGCC 9 cut(s) 146, 811, 1033, 1861, 2242, 2352, 2396, 2459, 2528
BtgI CCRYGG 2 cut(s) 141, 2059
BtgZI GCGATG 2 cut(s) 855, 1077
BtsI GCAGTG 3 cut(s) 12, 33, 35
BveI ACCTGC 1 cut(s) 2374
Cac8I GCNNGC 6 cut(s) 60, 1286, 1838, 2180, 2354, 2394
CaiI CAGNNNCTG 1 cut(s) 1421
CciI TCATGA 2 cut(s) 1657, 2146
CfoI GCGC 1 cut(s) 2371
Cfr10I RCCGGY 2 cut(s) 1284, 1931
Cfr13I GGNCC 7 cut(s) 809, 1031, 1320, 1595, 2241, 2457, 2458
Cfr42I CCGCGG 1 cut(s) 2062
CseI GACGC 1 cut(s) 2179
Csp6I GTAC 8 cut(s) 504, 857, 1079, 1990, 2119, 2224, 2296, 2500
CspAI ACCGGT 1 cut(s) 1931
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviQI GTAC 8 cut(s) 504, 857, 1079, 1990, 2119, 2224, 2296, 2500
EaeI YGGCCR 2 cut(s) 144, 2350
Eam1104I CTCTTC 2 cut(s) 198, 2189
EarI CTCTTC 2 cut(s) 198, 2189
Ecl136II GAGCTC 2 cut(s) 208, 718
Eco130I CCWWGG 2 cut(s) 735, 2529
Eco147I AGGCCT 1 cut(s) 2396
Eco24I GRGCYC 3 cut(s) 210, 720, 2461
Eco31I GGTCTC 4 cut(s) 188, 327, 467, 503
Eco47I GGWCC 2 cut(s) 1320, 1595
Eco53kI GAGCTC 2 cut(s) 208, 718
Eco81I CCTNAGG 2 cut(s) 1809, 2291
Eco88I CYCGRG 2 cut(s) 2122, 2282
EcoICRI GAGCTC 2 cut(s) 208, 718
EcoNI CCTNNNNNAGG 2 cut(s) 785, 1007
EcoO109I RGGNCCY 5 cut(s) 809, 1031, 2241, 2457, 2458
EcoRI GAATTC 2 cut(s) 484, 2087
EcoRII CCWGG 8 cut(s) 171, 931, 1153, 1730, 2331, 2396, 2406, 2569
EcoT14I CCWWGG 2 cut(s) 735, 2529
EcoT38I GRGCYC 3 cut(s) 210, 720, 2461
ErhI CCWWGG 2 cut(s) 735, 2529
FaqI GGGAC 7 cut(s) 91, 1189, 1333, 1508, 1904, 2118, 2216
FauI CCCGC 1 cut(s) 2054
FauNDI CATATG 1 cut(s) 1682
FbaI TGATCA 2 cut(s) 627, 1654
Fnu4HI GCNGC 9 cut(s) 280, 449, 1834, 1869, 1910, 2156, 2159, 2162, 2436
FriOI GRGCYC 3 cut(s) 210, 720, 2461
Fsp4HI GCNGC 9 cut(s) 280, 449, 1834, 1869, 1910, 2156, 2159, 2162, 2436
FspBI CTAG 6 cut(s) 363, 608, 813, 1035, 2481, 2535
GlaI GCGC 1 cut(s) 2370
GluI GCNGC 9 cut(s) 280, 449, 1834, 1869, 1910, 2156, 2159, 2162, 2436
GsaI CCCAGC 3 cut(s) 747, 1210, 1909
GsuI CTGGAG 6 cut(s) 137, 954, 1176, 1596, 1753, 2315
HaeIII GGCC 9 cut(s) 146, 811, 1033, 1861, 2242, 2352, 2396, 2459, 2528
HapII CCGG 6 cut(s) 674, 1199, 1285, 1932, 2357, 2389
HgaI GACGC 1 cut(s) 2179
HhaI GCGC 1 cut(s) 2371
Hin6I GCGC 1 cut(s) 2369
HinP1I GCGC 1 cut(s) 2369
HpaII CCGG 6 cut(s) 674, 1199, 1285, 1932, 2357, 2389
HphI GGTGA 8 cut(s) 763, 942, 1164, 1433, 1688, 1797, 1960, 2048
Hpy166II GTNNAC 2 cut(s) 33, 1716
Hpy8I GTNNAC 2 cut(s) 33, 1716
HpyCH4III ACNGT 3 cut(s) 1102, 1702, 2453
HpyCH4IV ACGT 2 cut(s) 431, 1256
HpyF10VI GCNNNNNNNGC 9 cut(s) 214, 2161, 2170, 2179, 2188, 2248, 2271, 2368, 2486
HpySE526I ACGT 2 cut(s) 431, 1256
HspAI GCGC 1 cut(s) 2369
KpnI GGTACC 1 cut(s) 2299
KroI GCCGGC 1 cut(s) 1284
KroNI GCCGGC 1 cut(s) 1286
Ksp22I TGATCA 2 cut(s) 627, 1654
KspI CCGCGG 1 cut(s) 2062
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 8 cut(s) 23, 715, 737, 935, 1157, 1406, 1615, 2359
Lsp1109I GCAGC 8 cut(s) 266, 460, 1820, 1855, 2167, 2170, 2173, 2422
LweI GCATC 7 cut(s) 873, 922, 1095, 1144, 1303, 2178, 2401
MaeI CTAG 6 cut(s) 363, 608, 813, 1035, 2481, 2535
MaeII ACGT 2 cut(s) 431, 1256
MaeIII GTNAC 6 cut(s) 12, 776, 998, 1676, 1803, 2167
MflI RGATCY 3 cut(s) 378, 592, 1780
MhlI GDGCHC 4 cut(s) 210, 720, 734, 2461
MlyI GAGTC 6 cut(s) 128, 1211, 1532, 1575, 2134, 2140
MmeI TCCRAC 2 cut(s) 318, 1312
MroNI GCCGGC 1 cut(s) 1284
MroXI GAANNNNTTC 2 cut(s) 1749, 2213
MseI TTAA 7 cut(s) 54, 284, 348, 1380, 1436, 1895, 1941
MspA1I CMGCKG 1 cut(s) 2061
MspCI CTTAAG 2 cut(s) 283, 347
MspI CCGG 6 cut(s) 674, 1199, 1285, 1932, 2357, 2389
MspR9I CCNGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
MvaI CCWGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
MvnI CGCG 2 cut(s) 405, 2061
MwoI GCNNNNNNNGC 9 cut(s) 214, 2161, 2170, 2179, 2188, 2248, 2271, 2368, 2486
NaeI GCCGGC 1 cut(s) 1286
NdeI CATATG 1 cut(s) 1682
NgoMIV GCCGGC 1 cut(s) 1284
NmuCI GTSAC 3 cut(s) 1676, 1803, 2167
NspI RCATGY 1 cut(s) 1833
NspV TTCGAA 1 cut(s) 1743
PaeR7I CTCGAG 2 cut(s) 2122, 2282
PagI TCATGA 2 cut(s) 1657, 2146
PaqCI CACCTGC 1 cut(s) 2374
PceI AGGCCT 1 cut(s) 2396
PciSI GCTCTTC 1 cut(s) 198
PdiI GCCGGC 1 cut(s) 1286
PdmI GAANNNNTTC 2 cut(s) 1749, 2213
PfeI GAWTC 5 cut(s) 343, 611, 1121, 2542, 2588
PfoI TCCNGGA 2 cut(s) 931, 1153
PinAI ACCGGT 1 cut(s) 1931
PkrI GCNGC 9 cut(s) 281, 450, 1835, 1870, 1911, 2157, 2160, 2163, 2437
PleI GAGTC 6 cut(s) 127, 1210, 1531, 1575, 2133, 2139
PpsI GAGTC 6 cut(s) 127, 1210, 1531, 1575, 2133, 2139
PshAI GACNNNNGTC 1 cut(s) 2321
PshBI ATTAAT 1 cut(s) 1941
Psp124BI GAGCTC 2 cut(s) 210, 720
Psp1406I AACGTT 1 cut(s) 431
Psp6I CCWGG 8 cut(s) 171, 931, 1153, 1730, 2331, 2396, 2406, 2569
PspFI CCCAGC 3 cut(s) 743, 1206, 1905
PspGI CCWGG 8 cut(s) 171, 931, 1153, 1730, 2331, 2396, 2406, 2569
PspOMI GGGCCC 1 cut(s) 2457
PspPI GGNCC 7 cut(s) 809, 1031, 1320, 1595, 2241, 2457, 2458
PspXI VCTCGAGB 1 cut(s) 2122
PstI CTGCAG 3 cut(s) 450, 496, 1855
PstNI CAGNNNCTG 1 cut(s) 1421
PsuI RGATCY 3 cut(s) 378, 592, 1780
RsaI GTAC 8 cut(s) 505, 858, 1080, 1991, 2120, 2225, 2297, 2501
RsaNI GTAC 8 cut(s) 504, 857, 1079, 1990, 2119, 2224, 2296, 2500
SacI GAGCTC 2 cut(s) 210, 720
SacII CCGCGG 1 cut(s) 2062
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 7 cut(s) 54, 284, 348, 1380, 1436, 1895, 1941
SatI GCNGC 9 cut(s) 280, 449, 1834, 1869, 1910, 2156, 2159, 2162, 2436
Sau96I GGNCC 7 cut(s) 809, 1031, 1320, 1595, 2241, 2457, 2458
ScaI AGTACT 1 cut(s) 2225
SchI GAGTC 6 cut(s) 128, 1211, 1532, 1575, 2134, 2140
ScrFI CCNGG 8 cut(s) 173, 933, 1155, 1732, 2333, 2398, 2408, 2571
SduI GDGCHC 4 cut(s) 210, 720, 734, 2461
SfaNI GCATC 7 cut(s) 873, 922, 1095, 1144, 1303, 2178, 2401
SfcI CTRYAG 4 cut(s) 446, 492, 1098, 1851
Sfr274I CTCGAG 2 cut(s) 2122, 2282
Sfr303I CCGCGG 1 cut(s) 2062
SfuI TTCGAA 1 cut(s) 1743
SgrBI CCGCGG 1 cut(s) 2062
SinI GGWCC 2 cut(s) 1320, 1595
SlaI CTCGAG 2 cut(s) 2122, 2282
SmlI CTYRAG 8 cut(s) 283, 326, 347, 596, 1289, 2122, 2282, 2503
SmoI CTYRAG 8 cut(s) 283, 326, 347, 596, 1289, 2122, 2282, 2503
SseBI AGGCCT 1 cut(s) 2396
SsiI CCGC 4 cut(s) 403, 1910, 2059, 2061
SspI AATATT 3 cut(s) 1303, 1313, 1400
SspMI CTAG 6 cut(s) 363, 608, 813, 1035, 2481, 2535
SstI GAGCTC 2 cut(s) 210, 720
StuI AGGCCT 1 cut(s) 2396
StyD4I CCNGG 8 cut(s) 171, 931, 1153, 1730, 2331, 2396, 2406, 2569
StyI CCWWGG 2 cut(s) 735, 2529
TaaI ACNGT 3 cut(s) 1102, 1702, 2453
TaiI ACGT 2 cut(s) 434, 1259
TaqI TCGA 6 cut(s) 482, 1743, 2073, 2123, 2283, 2301
TaqII GACCGA 1 cut(s) 1337
TatI WGTACW 2 cut(s) 503, 2223
TauI GCSGC 1 cut(s) 1912
TfiI GAWTC 5 cut(s) 343, 611, 1121, 2542, 2588
Tru1I TTAA 7 cut(s) 54, 284, 348, 1380, 1436, 1895, 1941
Tru9I TTAA 7 cut(s) 54, 284, 348, 1380, 1436, 1895, 1941
TseFI GTSAC 3 cut(s) 1676, 1803, 2167
TseI GCWGC 8 cut(s) 279, 448, 1833, 1868, 2155, 2158, 2161, 2435
Tsp45I GTSAC 3 cut(s) 1676, 1803, 2167
TspDTI ATGAA 7 cut(s) 819, 858, 921, 1041, 1080, 1229, 1281
TspGWI ACGGA 2 cut(s) 552, 1991
Vha464I CTTAAG 2 cut(s) 283, 347
VpaK11BI GGWCC 2 cut(s) 1320, 1595
VspI ATTAAT 1 cut(s) 1941
XagI CCTNNNNNAGG 2 cut(s) 785, 1007
XapI RAATTY 3 cut(s) 484, 1951, 2087
XceI RCATGY 1 cut(s) 1833
XcmI CCANNNNNNNNNTGG 2 cut(s) 1400, 1451
XhoI CTCGAG 2 cut(s) 2122, 2282
XmnI GAANNNNTTC 2 cut(s) 1749, 2213
XspI CTAG 6 cut(s) 363, 608, 813, 1035, 2481, 2535
ZrmI AGTACT 1 cut(s) 2225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.