Rmu_sc0003825.1_g000016

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003825.1
OG0000408
Physical Location & Seq
Reverse (-)
51816 .. 57449
5634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003825.1_g000016.1.cds

Sequence Viewer

Length: 3015 bp
atggcagtggagtttctcggaggagcagtatttacagtgctgtatgatggggttaagcaagcaatcggaaagacagggactttcaaataccttctcaaagacatcaaatccacactggagtctttgaacaagctacacgccacggccatcagacaaatcggagagtataacctggaattgggtctcccaaatgatgatatagaagagctcaaagagcaaatggaggaaggggcaaagctggtgggaaaattgtctgattttcgtgtgtggaactactgctatttaagttgttataccgatcaacttgttgatttggataggtctctcaagaggctgttggagaatctaaagttgcaagaaactagagatgtgaaagagatcttggatttgctgaggaaataccgcgttgagcttgatgagttgaagggaacgttgcagatattactgcagcagaaagcaggagatgtgaaggagaccacctcgaattcaactgcaggaagcagtacagagaccatgcctcagcaaaatgtagggaacggagaggaacaagtaggggcagatcttggtgcagtatacaggatgctgtttgatgttgttacagaagtgaaggacaagactttgatgttcagacccctactcggagatctcaagtccatgctagattccttaaaaccactgatcgaagagattaaacaatacaataatgtattggttctcccaaagccggaggtagaaaagttcaggacacaaatggacaagggtgtggagctcgttcgcaagtgctccaaggttcatccatgggccagctacaaaaagtacaattacacaatccaacttattggattgaatgaatctgttcgattactgttaagtagactgagagagcaggtagctggggaagtgagtgagacattggctgcaataagtaatctaaaggaggtagtcaagcaatatgaagggcctagtgtcatacaaaatcaacatgagattatacgttcctgcaaagtacctgaaccttcatcgcctacattgatgcctgagattgaggattctgttgaagtgcatccattaccagtgcctcctggagcaaaactaccttcaccaaaggaggtagttgaggaagtgagtgagacattggcttcaataagtaacctaaaggaggtagtcaagcaatatgaagggcctagtgtcatacaaaatcaacatgagattatacgttcctgcaaagtacccgaaccttcatcgcctacagtgatgcctgagattgaggattctgttgaagtgcatccattaccagtgcctcctggagcaaaactaccttcaccaaaggttctaattacatctgaaccggagtcccagccaatgaaaggtcaatggcaaaagggaaagcttattggtcttggcacgtttgggaatgtttatgttgattccaatggagaaactggagctttacgtgcaatgaaggaagttgatttcattcctgatgacccaaaatctgccgagtttatgaagcagctacagcagccagcccctattcaagtagcagacaatcagatgattttagcagatctctacgtggttaaagtcattggtaaaggaaatggtgggattgtgcaattggttcagcacaaatggactggccagttttttgcattgaaggtaatccaaatgaatattcaggtgtctattggcaagcagattgcacaagaattgaaaattaatcaatcaaaacaatgtccctatattgttgcatgttaccagtccttctatgataatggttacgtttcaagtatcctagagtacatggatggtggatctttagcagatcttctgagaaaagtcagatcaattccagagccatatatttctgctatgtgtaagcagttgctgcgcggtttgttgtatcttcatcatgaagaacacataattcacagggacttcaaaccctcgaacttgttaataaatcatagaggagaaattaagataaatgactttggtgtcagtgcaaaaaaggcaaacacctctgaacgggcaaatacctttgttggcacatacaactatatgtcgccagagagaatacaaggtaacagctatagctacaaaagtgacatttggagcttgggcttagtactactggagtgtgcaacaggtaaattcccatatacaccaccagatcaaagtgaaggatgggaaaacttttttgagcttatgagtgctgttgttgacttgccaccaccttctgcaccttccgatcagttttctccagagttctgctcatttatttctgcatgtgtacggaaagatcctgaaaagagattgtcagcacatgatctcctagtgcatccttttatcagcatgtacgacgacttgaatatcgacctttcgtcttacttcactgaagcaggcgatatcgaagcaggcgatatcgacctttcgtcttacttcgctgaagcaggcgatatcaacctttcgtcttacttcactgaagcaggcgatatttctccctctcggcccagccgcctcctacgtcttcgctgcccctgcagccttctcggaccaccacacaccactgctgcccctgcaacacagcaggacgctcgtccctgtgcagatcagcttgcttgcacgccccgaaacgtcttgatcgggacctcagatcaaaatatttccttcatcaaagttgttcgtctctgtctcttctatctaacctccaaatttcagccttatcggagttgtcttgagacctatacaccaatcgaagtgaaaactgttcagaggcaaatctgctccgaatttcaacaaaaaatccttgaaaaccgccgctgccaccttcaagcatcactgcagcagctcctagcccacgctagcctcatctacgtgcctgcccctgcaacgcctacgcgcccctgcgcacgcatcagtcggccagcccctcgacttaccttggccagacttgtatcggccacacagcagggattgaaagatagtttgcaatcccagacccaggatcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009628 GO:0009631 GO:0009651 GO:0009814 GO:0009893 GO:0009914 GO:0009966 GO:0009967 GO:0009987 GO:0010051 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1004

Amino Acids

112.24

Weight (kDa)

5.76

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1997
Acc16I TGCGCA 1 cut(s) 2911
Acc36I ACCTGC 1 cut(s) 877
AccI GTMKAC 2 cut(s) 573, 874
AccII CGCG 3 cut(s) 405, 1893, 2902
AciI CCGC 5 cut(s) 403, 1893, 2518, 2818, 2821
AclI AACGTT 1 cut(s) 431
AclWI GGATC 2 cut(s) 1822, 2297
AcoI YGGCCR 5 cut(s) 144, 1639, 2924, 2946, 2961
AcsI RAATTY 4 cut(s) 484, 2153, 2714, 2792
AcuI CTGAAG 3 cut(s) 2418, 2469, 2505
AfaI GTAC 8 cut(s) 505, 818, 1008, 1230, 1802, 2130, 2295, 2360
AfiI CCNNNNNNNGG 7 cut(s) 178, 638, 724, 1159, 1367, 2028, 3004
AhdI GACNNNNNGTC 2 cut(s) 2383, 2434
AjnI CCWGG 4 cut(s) 171, 1081, 1303, 3003
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 1659, 1691
Alw21I GWGCWC 3 cut(s) 210, 771, 785
Alw26I GTCTC 9 cut(s) 188, 327, 467, 503, 902, 1124, 2693, 2699, 2735
AlwI GGATC 2 cut(s) 1822, 2297
AlwNI CAGNNNCTG 1 cut(s) 1888
AoxI GGCC 9 cut(s) 144, 801, 959, 1181, 1639, 2510, 2924, 2946, 2961
ApoI RAATTY 4 cut(s) 484, 2153, 2714, 2792
AseI ATTAAT 1 cut(s) 1719
Asp700I GAANNNNTTC 1 cut(s) 855
AspLEI GCGC 3 cut(s) 1893, 2904, 2912
AspS9I GGNCC 6 cut(s) 801, 959, 1181, 2511, 2555, 2649
AsuHPI GGTGA 2 cut(s) 1092, 1314
AsuNHI GCTAGC 1 cut(s) 2864
AvaII GGWCC 2 cut(s) 2555, 2649
BalI TGGCCA 2 cut(s) 1641, 2948
BanII GRGCYC 2 cut(s) 210, 771
BbsI GAAGAC 1 cut(s) 2522
Bbv12I GWGCWC 3 cut(s) 210, 771, 785
BbvCI CCTCAGC 2 cut(s) 392, 519
BccI CCATC 4 cut(s) 41, 155, 1802, 2181
BceAI ACGGC 1 cut(s) 159
BcgI CGANNNNNNTGC 4 cut(s) 2533, 2567, 2579, 2613
BciT130I CCWGG 4 cut(s) 173, 1083, 1305, 3005
BciVI GTATCC 1 cut(s) 1802
BcoDI GTCTC 9 cut(s) 188, 327, 467, 503, 902, 1124, 2693, 2699, 2735
BfaI CTAG 8 cut(s) 363, 659, 963, 1185, 1796, 2336, 2855, 2865
BfmI CTRYAG 7 cut(s) 446, 492, 1248, 1517, 2092, 2542, 2843
BfuAI ACCTGC 1 cut(s) 877
BfuI GTATCC 1 cut(s) 1802
BglII AGATCT 5 cut(s) 378, 559, 643, 1567, 1825
BmcAI AGTACT 1 cut(s) 2130
Bme1390I CCNGG 4 cut(s) 173, 1083, 1305, 3005
Bme18I GGWCC 2 cut(s) 2555, 2649
BmeRI GACNNNNNGTC 2 cut(s) 2383, 2434
BmgT120I GGNCC 6 cut(s) 801, 959, 1181, 2511, 2555, 2649
BmiI GGNNCC 1 cut(s) 2650
BmrFI CCNGG 4 cut(s) 173, 1083, 1305, 3005
BmsI GCATC 8 cut(s) 570, 1023, 1072, 1245, 1294, 2350, 2846, 2925
BmtI GCTAGC 1 cut(s) 2868
BoxI GACNNNNGTC 1 cut(s) 2598
BpiI GAAGAC 1 cut(s) 2522
BplI GAGNNNNNCTC 4 cut(s) 464, 496, 2258, 2290
BpmI CTGGAG 6 cut(s) 137, 1104, 1326, 1464, 2156, 2247
Bpu10I CCTNAGC 2 cut(s) 392, 519
BpuEI CTTGAG 3 cut(s) 311, 632, 2759
BsaAI YACGTR 3 cut(s) 1454, 1576, 2878
BsaI GGTCTC 5 cut(s) 188, 327, 467, 503, 2735
BsaJI CCNNGG 5 cut(s) 141, 786, 797, 2943, 3003
BsaWI WCCGGW 1 cut(s) 1348
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 2316, 2346
Bsc4I CCNNNNNNNGG 7 cut(s) 178, 638, 724, 1159, 1367, 2028, 3004
Bse1I ACTGG 8 cut(s) 120, 1073, 1295, 1447, 1642, 1642, 1759, 2139
Bse3DI GCAATG 1 cut(s) 1464
BseBI CCWGG 4 cut(s) 173, 1083, 1305, 3005
BseDI CCNNGG 5 cut(s) 141, 786, 797, 2943, 3003
BseGI GGATG 7 cut(s) 585, 793, 1063, 1285, 1813, 2192, 2341
BseLI CCNNNNNNNGG 7 cut(s) 178, 638, 724, 1159, 1367, 2028, 3004
BseMI GCAATG 1 cut(s) 1464
BseMII CTCAG 7 cut(s) 383, 533, 869, 1029, 1251, 1823, 2667
BseNI ACTGG 8 cut(s) 120, 1073, 1295, 1447, 1642, 1642, 1759, 2139
BseRI GAGGAG 2 cut(s) 36, 1986
BseYI CCCAGC 3 cut(s) 893, 1356, 2513
BsgI GTGCAG 3 cut(s) 588, 2226, 2628
Bsh1236I CGCG 3 cut(s) 405, 1893, 2902
BshFI GGCC 9 cut(s) 146, 803, 961, 1183, 1641, 2512, 2926, 2948, 2963
BsiHKAI GWGCWC 3 cut(s) 210, 771, 785
BsiSI CCGG 2 cut(s) 725, 1349
BslFI GGGAC 6 cut(s) 91, 1339, 1722, 1949, 2586, 2662
BslI CCNNNNNNNGG 7 cut(s) 178, 638, 724, 1159, 1367, 2028, 3004
BsmAI GTCTC 9 cut(s) 188, 327, 467, 503, 902, 1124, 2693, 2699, 2735
BsmBI CGTCTC 1 cut(s) 2693
BsmFI GGGAC 6 cut(s) 91, 1339, 1722, 1949, 2586, 2662
BsnI GGCC 9 cut(s) 146, 803, 961, 1183, 1641, 2512, 2926, 2948, 2963
Bso31I GGTCTC 5 cut(s) 188, 327, 467, 503, 2735
Bsp1286I GDGCHC 3 cut(s) 210, 771, 785
Bsp19I CCATGG 1 cut(s) 797
BspACI CCGC 5 cut(s) 403, 1893, 2518, 2818, 2821
BspANI GGCC 9 cut(s) 146, 803, 961, 1183, 1641, 2512, 2926, 2948, 2963
BspCNI CTCAG 7 cut(s) 384, 532, 870, 1030, 1252, 1824, 2666
BspFNI CGCG 3 cut(s) 405, 1893, 2902
BspHI TCATGA 1 cut(s) 1912
BspLI GGNNCC 1 cut(s) 2650
BspMAI CTGCAG 4 cut(s) 450, 496, 2546, 2847
BspMI ACCTGC 1 cut(s) 877
BspOI GCTAGC 1 cut(s) 2868
BspPI GGATC 2 cut(s) 1822, 2297
BspQI GCTCTTC 1 cut(s) 198
BspTNI GGTCTC 5 cut(s) 188, 327, 467, 503, 2735
BsrDI GCAATG 1 cut(s) 1464
BsrI ACTGG 8 cut(s) 120, 1073, 1295, 1447, 1642, 1642, 1759, 2139
BssECI CCNNGG 5 cut(s) 141, 786, 797, 2943, 3003
BssNAI GTATAC 1 cut(s) 574
BssT1I CCWWGG 3 cut(s) 786, 797, 2943
Bst1107I GTATAC 1 cut(s) 574
Bst2UI CCWGG 4 cut(s) 173, 1083, 1305, 3005
Bst4CI ACNGT 4 cut(s) 37, 867, 1252, 2770
Bst6I CTCTTC 3 cut(s) 198, 678, 2702
BstAPI GCANNNNNTGC 1 cut(s) 1888
BstBAI YACGTR 3 cut(s) 1454, 1576, 2878
BstDEI CTNAG 8 cut(s) 392, 519, 878, 1038, 1260, 1832, 2125, 2653
BstDSI CCRYGG 2 cut(s) 141, 797
BstENI CCTNNNNNAGG 1 cut(s) 1157
BstF5I GGATG 7 cut(s) 585, 793, 1063, 1285, 1813, 2192, 2341
BstFNI CGCG 3 cut(s) 405, 1893, 2902
BstHHI GCGC 3 cut(s) 1893, 2904, 2912
BstMAI GTCTC 9 cut(s) 188, 327, 467, 503, 902, 1124, 2693, 2699, 2735
BstMWI GCNNNNNNNGC 9 cut(s) 214, 1454, 1519, 1522, 1888, 2012, 2541, 2544, 2579
BstNI CCWGG 4 cut(s) 173, 1083, 1305, 3005
BstNSI RCATGY 3 cut(s) 1755, 2292, 2359
BstPAI GACNNNNGTC 1 cut(s) 2598
BstSCI CCNGG 4 cut(s) 171, 1081, 1303, 3003
BstSFI CTRYAG 7 cut(s) 446, 492, 1248, 1517, 2092, 2542, 2843
BstUI CGCG 3 cut(s) 405, 1893, 2902
BstV2I GAAGAC 1 cut(s) 2522
BstX2I RGATCY 7 cut(s) 378, 559, 643, 1567, 1814, 1825, 2302
BstXI CCANNNNNNTGG 1 cut(s) 839
BstYI RGATCY 7 cut(s) 378, 559, 643, 1567, 1814, 1825, 2302
BstZ17I GTATAC 1 cut(s) 574
BsuI GTATCC 1 cut(s) 1802
BsuRI GGCC 9 cut(s) 146, 803, 961, 1183, 1641, 2512, 2926, 2948, 2963
BtgI CCRYGG 2 cut(s) 141, 797
BtgZI GCGATG 2 cut(s) 1005, 1227
BtsCI GGATG 7 cut(s) 585, 793, 1063, 1285, 1813, 2192, 2341
BtsI GCAGTG 3 cut(s) 12, 2568, 2840
BveI ACCTGC 1 cut(s) 877
CaiI CAGNNNCTG 1 cut(s) 1888
CciI TCATGA 1 cut(s) 1912
CfoI GCGC 3 cut(s) 1893, 2904, 2912
Cfr13I GGNCC 6 cut(s) 801, 959, 1181, 2511, 2555, 2649
CseI GACGC 1 cut(s) 2603
Csp6I GTAC 8 cut(s) 504, 817, 1007, 1229, 1801, 2129, 2294, 2359
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviQI GTAC 8 cut(s) 504, 817, 1007, 1229, 1801, 2129, 2294, 2359
DdeI CTNAG 8 cut(s) 392, 519, 878, 1038, 1260, 1832, 2125, 2653
DrdI GACNNNNNNGTC 1 cut(s) 1997
DriI GACNNNNNGTC 2 cut(s) 2383, 2434
DseDI GACNNNNNNGTC 1 cut(s) 1997
EaeI YGGCCR 5 cut(s) 144, 1639, 2924, 2946, 2961
Eam1104I CTCTTC 3 cut(s) 198, 678, 2702
Eam1105I GACNNNNNGTC 2 cut(s) 2383, 2434
EarI CTCTTC 3 cut(s) 198, 678, 2702
Ecl136II GAGCTC 2 cut(s) 208, 769
Eco130I CCWWGG 3 cut(s) 786, 797, 2943
Eco24I GRGCYC 2 cut(s) 210, 771
Eco31I GGTCTC 5 cut(s) 188, 327, 467, 503, 2735
Eco32I GATATC 3 cut(s) 2410, 2425, 2461
Eco47I GGWCC 2 cut(s) 2555, 2649
Eco53kI GAGCTC 2 cut(s) 208, 769
Eco57I CTGAAG 3 cut(s) 2418, 2469, 2505
EcoICRI GAGCTC 2 cut(s) 208, 769
EcoNI CCTNNNNNAGG 1 cut(s) 1157
EcoO109I RGGNCCY 3 cut(s) 959, 1181, 2649
EcoRI GAATTC 1 cut(s) 484
EcoRII CCWGG 4 cut(s) 171, 1081, 1303, 3003
EcoRV GATATC 3 cut(s) 2410, 2425, 2461
EcoT14I CCWWGG 3 cut(s) 786, 797, 2943
EcoT38I GRGCYC 2 cut(s) 210, 771
ErhI CCWWGG 3 cut(s) 786, 797, 2943
Esp3I CGTCTC 1 cut(s) 2693
FaqI GGGAC 6 cut(s) 91, 1339, 1722, 1949, 2586, 2662
FblI GTMKAC 2 cut(s) 573, 874
FokI GGATG 7 cut(s) 592, 780, 1050, 1272, 1820, 2199, 2328
FriOI GRGCYC 2 cut(s) 210, 771
FspBI CTAG 8 cut(s) 363, 659, 963, 1185, 1796, 2336, 2855, 2865
FspI TGCGCA 1 cut(s) 2911
GlaI GCGC 3 cut(s) 1892, 2903, 2911
GsaI CCCAGC 3 cut(s) 897, 1360, 2517
GsuI CTGGAG 6 cut(s) 137, 1104, 1326, 1464, 2156, 2247
HaeIII GGCC 9 cut(s) 146, 803, 961, 1183, 1641, 2512, 2926, 2948, 2963
HapII CCGG 2 cut(s) 725, 1349
HgaI GACGC 1 cut(s) 2603
HhaI GCGC 3 cut(s) 1893, 2904, 2912
Hin6I GCGC 3 cut(s) 1891, 2902, 2910
HinP1I GCGC 3 cut(s) 1891, 2902, 2910
HincII GTYRAC 1 cut(s) 2224
HindII GTYRAC 1 cut(s) 2224
HindIII AAGCTT 1 cut(s) 1388
HinfI GANTC 8 cut(s) 119, 343, 662, 851, 1049, 1271, 1352, 1427
HpaII CCGG 2 cut(s) 725, 1349
HphI GGTGA 2 cut(s) 1092, 1314
Hpy166II GTNNAC 4 cut(s) 574, 875, 2224, 2294
Hpy8I GTNNAC 4 cut(s) 574, 875, 2224, 2294
Hpy99I CGWCG 1 cut(s) 2366
HpyCH4III ACNGT 4 cut(s) 37, 867, 1252, 2770
HpyF10VI GCNNNNNNNGC 9 cut(s) 214, 1454, 1519, 1522, 1888, 2012, 2541, 2544, 2579
HpyF3I CTNAG 8 cut(s) 392, 519, 878, 1038, 1260, 1832, 2125, 2653
HspAI GCGC 3 cut(s) 1891, 2902, 2910
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 9 cut(s) 23, 766, 788, 1085, 1307, 1445, 2115, 2792, 2856
LweI GCATC 8 cut(s) 570, 1023, 1072, 1245, 1294, 2350, 2846, 2925
MaeI CTAG 8 cut(s) 363, 659, 963, 1185, 1796, 2336, 2855, 2865
MaeIII GTNAC 6 cut(s) 595, 1148, 1754, 1778, 2084, 2105
MboII GAAGA 7 cut(s) 215, 695, 1820, 1898, 1928, 2522, 2689
MfeI CAATTG 1 cut(s) 1616
MflI RGATCY 7 cut(s) 378, 559, 643, 1567, 1814, 1825, 2302
MhlI GDGCHC 3 cut(s) 210, 771, 785
MlsI TGGCCA 2 cut(s) 1641, 2948
MluNI TGGCCA 2 cut(s) 1641, 2948
MlyI GAGTC 2 cut(s) 128, 1361
MmeI TCCRAC 2 cut(s) 318, 856
Mox20I TGGCCA 2 cut(s) 1641, 2948
MroXI GAANNNNTTC 1 cut(s) 855
MscI TGGCCA 2 cut(s) 1641, 2948
MseI TTAA 9 cut(s) 54, 284, 668, 690, 869, 1581, 1719, 1958, 1980
MslI CAYNNNNRTG 1 cut(s) 2876
Msp20I TGGCCA 2 cut(s) 1641, 2948
MspA1I CMGCKG 1 cut(s) 2823
MspI CCGG 2 cut(s) 725, 1349
MspR9I CCNGG 4 cut(s) 173, 1083, 1305, 3005
MunI CAATTG 1 cut(s) 1616
MvaI CCWGG 4 cut(s) 173, 1083, 1305, 3005
MvnI CGCG 3 cut(s) 405, 1893, 2902
MwoI GCNNNNNNNGC 9 cut(s) 214, 1454, 1519, 1522, 1888, 2012, 2541, 2544, 2579
NcoI CCATGG 1 cut(s) 797
NheI GCTAGC 1 cut(s) 2864
NlaIV GGNNCC 1 cut(s) 2650
NmeAIII GCCGAG 2 cut(s) 1525, 2488
NmuCI GTSAC 1 cut(s) 2105
NsbI TGCGCA 1 cut(s) 2911
NspI RCATGY 3 cut(s) 1755, 2292, 2359
PagI TCATGA 1 cut(s) 1912
PciSI GCTCTTC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 2418
PdmI GAANNNNTTC 1 cut(s) 855
PfeI GAWTC 6 cut(s) 343, 662, 851, 1049, 1271, 1427
PfoI TCCNGGA 2 cut(s) 1081, 1303
PleI GAGTC 2 cut(s) 127, 1360
PpsI GAGTC 2 cut(s) 127, 1360
Ppu21I YACGTR 3 cut(s) 1454, 1576, 2878
PpuMI RGGWCCY 1 cut(s) 2649
PshAI GACNNNNGTC 1 cut(s) 2598
PshBI ATTAAT 1 cut(s) 1719
Psp124BI GAGCTC 2 cut(s) 210, 771
Psp1406I AACGTT 1 cut(s) 431
Psp5II RGGWCCY 1 cut(s) 2649
Psp6I CCWGG 4 cut(s) 171, 1081, 1303, 3003
PspFI CCCAGC 3 cut(s) 893, 1356, 2513
PspGI CCWGG 4 cut(s) 171, 1081, 1303, 3003
PspN4I GGNNCC 1 cut(s) 2650
PspPI GGNCC 6 cut(s) 801, 959, 1181, 2511, 2555, 2649
PspPPI RGGWCCY 1 cut(s) 2649
PstI CTGCAG 4 cut(s) 450, 496, 2546, 2847
PstNI CAGNNNCTG 1 cut(s) 1888
PsuI RGATCY 7 cut(s) 378, 559, 643, 1567, 1814, 1825, 2302
RsaI GTAC 8 cut(s) 505, 818, 1008, 1230, 1802, 2130, 2295, 2360
RsaNI GTAC 8 cut(s) 504, 817, 1007, 1229, 1801, 2129, 2294, 2359
RseI CAYNNNNRTG 1 cut(s) 2876
SacI GAGCTC 2 cut(s) 210, 771
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 9 cut(s) 54, 284, 668, 690, 869, 1581, 1719, 1958, 1980
Sau96I GGNCC 6 cut(s) 801, 959, 1181, 2511, 2555, 2649
ScaI AGTACT 1 cut(s) 2130
SchI GAGTC 2 cut(s) 128, 1361
ScrFI CCNGG 4 cut(s) 173, 1083, 1305, 3005
SduI GDGCHC 3 cut(s) 210, 771, 785
SfaNI GCATC 8 cut(s) 570, 1023, 1072, 1245, 1294, 2350, 2846, 2925
SfcI CTRYAG 7 cut(s) 446, 492, 1248, 1517, 2092, 2542, 2843
SinI GGWCC 2 cut(s) 2555, 2649
SmiMI CAYNNNNRTG 1 cut(s) 2876
SmlI CTYRAG 3 cut(s) 326, 647, 2738
SmoI CTYRAG 3 cut(s) 326, 647, 2738
SsiI CCGC 5 cut(s) 403, 1893, 2518, 2818, 2821
SspI AATATT 2 cut(s) 1675, 2665
SspMI CTAG 8 cut(s) 363, 659, 963, 1185, 1796, 2336, 2855, 2865
SstI GAGCTC 2 cut(s) 210, 771
StyD4I CCNGG 4 cut(s) 171, 1081, 1303, 3003
StyI CCWWGG 3 cut(s) 786, 797, 2943
TaaI ACNGT 4 cut(s) 37, 867, 1252, 2770
TaqI TCGA 9 cut(s) 482, 681, 859, 1949, 2376, 2412, 2427, 2757, 2935
TatI WGTACW 4 cut(s) 503, 816, 1800, 2128
TauI GCSGC 2 cut(s) 2520, 2823
TfiI GAWTC 6 cut(s) 343, 662, 851, 1049, 1271, 1427
Tru1I TTAA 9 cut(s) 54, 284, 668, 690, 869, 1581, 1719, 1958, 1980
Tru9I TTAA 9 cut(s) 54, 284, 668, 690, 869, 1581, 1719, 1958, 1980
TseFI GTSAC 1 cut(s) 2105
Tsp45I GTSAC 1 cut(s) 2105
TspGWI ACGGA 2 cut(s) 552, 2311
VpaK11BI GGWCC 2 cut(s) 2555, 2649
VspI ATTAAT 1 cut(s) 1719
XagI CCTNNNNNAGG 1 cut(s) 1157
XapI RAATTY 4 cut(s) 484, 2153, 2714, 2792
XceI RCATGY 3 cut(s) 1755, 2292, 2359
XmiI GTMKAC 2 cut(s) 573, 874
XmnI GAANNNNTTC 1 cut(s) 855
XspI CTAG 8 cut(s) 363, 659, 963, 1185, 1796, 2336, 2855, 2865
ZrmI AGTACT 1 cut(s) 2130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.