Rroxscaffold_3G00267990
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000408
Physical Location & Seq
Reverse (-)
61173160 .. 61174092
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267990.1

Sequence Viewer

Length: 786 bp
ATGCCGGCCAAGCTGATCGGAGGACCGCTGTACAATCAGCTCTTTGATGGCGTTAAGACAGCAAGAGGCAGAAATGGAGGAGGAGTGAAGCTGGTTGCCAAGCTATCAAGTGTTGGAATATGGAACTACCACTGCATGAAGAGTAACTACTCCGACCAACTAGCTCAATTGGATAGGTCTCTCAGAAAAATGTTATACATACTCAAGCTGCAGGAAGAAAGAGATATAATGGAGCTCTTGCTTGTTGCTAGGACAATACGGGACAGACAGAATGTTTTGGAAAGAAGACTATGTGGTTTACTCAAAGTGCAGCAGGAGAAAACCGATGGCAATGGTCACTTCGGCAGCGGAGGAGGAGGAGGAGGGGCTGGTCGGGATCTGGAGCATGTATTTTGCCGTCTACTTGAATCCCTTGTTTTGGAAGTTTATGTACAATTGAATGGGAAAACGAGGTTCAGTCCGGTCATGGCAGATCTGAAATCAACGGTTAGGTCCTTCCAACCACTCATGGAAGAAATATCAGATTATAACAAGTTATTGCATCTACCAAAGGAGGAACTAGACAATTTGAGGAAGCAACTGGAGAAAGGTATCGAGCTGGTCGACAAGTGCTCCAAGGTTCCTCAAGGGGCGAGCTACAAAAAGTATGAATACGCCGACGAACTACTGGAGTTGGATGACTATCTTCAGAGATTGTTGTGTATGATGAGGGGAGCAGGTAGCAAAGGATTTGAAGGAGACCTTGGTTTCTGTAAGCAACATGGAGATGATGTTAACGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

29.36

Weight (kDa)

8.12

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 138 - 232 2.8e-16 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 707
AccI GTMKAC 2 cut(s) 400, 603
AccII CGCG 1 cut(s) 779
AciI CCGC 3 cut(s) 26, 348, 779
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 6
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 2 cut(s) 32, 432
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 3 cut(s) 407, 439, 734
AjuI GAANNNNNNNTTGG 2 cut(s) 726, 758
AluBI AGCT 9 cut(s) 13, 40, 91, 103, 164, 208, 235, 598, 636
AluI AGCT 9 cut(s) 13, 40, 91, 103, 164, 208, 235, 598, 636
Alw21I GWGCWC 2 cut(s) 237, 614
Alw26I GTCTC 2 cut(s) 183, 732
AlwI GGATC 1 cut(s) 384
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 3 cut(s) 208, 310, 345
AspS9I GGNCC 2 cut(s) 23, 492
AvaII GGWCC 2 cut(s) 23, 492
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 4 cut(s) 131, 163, 414, 446
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 237, 614
BbvI GCAGC 3 cut(s) 195, 322, 357
BccI CCATC 2 cut(s) 41, 320
BceAI ACGGC 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 183, 732
BfaI CTAG 3 cut(s) 161, 249, 560
BfmI CTRYAG 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 707
BglII AGATCT 1 cut(s) 472
BisI GCNGC 3 cut(s) 209, 311, 346
BlsI GCNGC 3 cut(s) 210, 312, 347
Bme18I GGWCC 2 cut(s) 23, 492
BmgT120I GGNCC 2 cut(s) 23, 492
BmiI GGNNCC 1 cut(s) 621
BmsI GCATC 1 cut(s) 550
BpiI GAAGAC 1 cut(s) 292
BpmI CTGGAG 3 cut(s) 401, 602, 689
BpuEI CTTGAG 2 cut(s) 188, 609
BsaBI GATNNNNATC 1 cut(s) 681
BsaI GGTCTC 2 cut(s) 183, 732
BsaJI CCNNGG 2 cut(s) 615, 742
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 6 cut(s) 69, 99, 596, 626, 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 585, 672
Bse3DI GCAATG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 681
BseDI CCNNGG 2 cut(s) 615, 742
BseGI GGATG 1 cut(s) 682
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 196
BseNI ACTGG 2 cut(s) 585, 672
BseRI GAGGAG 6 cut(s) 93, 96, 366, 369, 372, 375
BseXI GCAGC 3 cut(s) 195, 322, 357
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 779
BshFI GGCC 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 237, 614
BsiSI CCGG 2 cut(s) 5, 461
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 418
BsmAI GTCTC 2 cut(s) 183, 732
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 2 cut(s) 183, 732
Bsp1286I GDGCHC 2 cut(s) 237, 614
Bsp1407I TGTACA 2 cut(s) 30, 430
Bsp143I GATC 3 cut(s) 15, 376, 472
BspACI CCGC 3 cut(s) 26, 348, 779
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 195
BspFNI CGCG 1 cut(s) 779
BspLI GGNNCC 1 cut(s) 621
BspMAI CTGCAG 1 cut(s) 213
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 1 cut(s) 384
BspTNI GGTCTC 2 cut(s) 183, 732
BsrDI GCAATG 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 2 cut(s) 30, 430
BsrI ACTGG 2 cut(s) 585, 672
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 615, 742
BssMI GATC 3 cut(s) 15, 376, 472
BssT1I CCWWGG 2 cut(s) 615, 742
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 1 cut(s) 134
BstAUI TGTACA 2 cut(s) 30, 430
BstC8I GCNNGC 2 cut(s) 6, 634
BstDEI CTNAG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 682
BstFNI CGCG 1 cut(s) 779
BstKTI GATC 3 cut(s) 18, 379, 475
BstMAI GTCTC 2 cut(s) 183, 732
BstMBI GATC 3 cut(s) 15, 376, 472
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 389
BstSFI CTRYAG 1 cut(s) 209
BstUI CGCG 1 cut(s) 779
BstV1I GCAGC 3 cut(s) 195, 322, 357
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 2 cut(s) 376, 472
BstYI RGATCY 2 cut(s) 376, 472
BsuRI GGCC 1 cut(s) 8
BtsCI GGATG 1 cut(s) 682
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 1 cut(s) 130
BveI ACCTGC 1 cut(s) 707
Cac8I GCNNGC 2 cut(s) 6, 634
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 2 cut(s) 23, 492
Csp6I GTAC 2 cut(s) 31, 431
CviAII CATG 5 cut(s) 136, 386, 466, 508, 761
CviQI GTAC 2 cut(s) 31, 431
DdeI CTNAG 1 cut(s) 182
DpnI GATC 3 cut(s) 17, 378, 474
DpnII GATC 3 cut(s) 15, 376, 472
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 615, 742
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 2 cut(s) 183, 732
Eco47I GGWCC 2 cut(s) 23, 492
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 671
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 492
EcoT14I CCWWGG 2 cut(s) 615, 742
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 2 cut(s) 615, 742
FaeI CATG 5 cut(s) 139, 389, 469, 511, 764
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 275
FatI CATG 5 cut(s) 135, 385, 465, 507, 760
FblI GTMKAC 2 cut(s) 400, 603
Fnu4HI GCNGC 3 cut(s) 209, 311, 346
FokI GGATG 1 cut(s) 689
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 3 cut(s) 209, 311, 346
FspBI CTAG 3 cut(s) 161, 249, 560
GluI GCNGC 3 cut(s) 209, 311, 346
GsuI CTGGAG 3 cut(s) 401, 602, 689
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 5, 461
Hin1II CATG 5 cut(s) 139, 389, 469, 511, 764
HincII GTYRAC 2 cut(s) 604, 775
HindII GTYRAC 2 cut(s) 604, 775
HinfI GANTC 1 cut(s) 407
HpaI GTTAAC 1 cut(s) 775
HpaII CCGG 2 cut(s) 5, 461
Hpy166II GTNNAC 4 cut(s) 299, 401, 604, 775
Hpy188I TCNGA 6 cut(s) 20, 154, 185, 477, 523, 690
Hpy188III TCNNGA 2 cut(s) 374, 380
Hpy8I GTNNAC 4 cut(s) 299, 401, 604, 775
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 2 cut(s) 505, 728
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4V TGCA 4 cut(s) 135, 211, 310, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 182
Hsp92II CATG 5 cut(s) 139, 389, 469, 511, 764
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
KspAI GTTAAC 1 cut(s) 775
Kzo9I GATC 3 cut(s) 15, 376, 472
LmnI GCTCC 4 cut(s) 232, 382, 617, 713
Lsp1109I GCAGC 3 cut(s) 195, 322, 357
LweI GCATC 1 cut(s) 550
MaeI CTAG 3 cut(s) 161, 249, 560
MaeIII GTNAC 2 cut(s) 143, 335
MalI GATC 3 cut(s) 17, 378, 474
MboI GATC 3 cut(s) 15, 376, 472
MboII GAAGA 5 cut(s) 151, 227, 297, 524, 677
MfeI CAATTG 2 cut(s) 167, 434
MflI RGATCY 2 cut(s) 376, 472
MhlI GDGCHC 2 cut(s) 237, 614
MluCI AATT 3 cut(s) 167, 434, 565
MmeI TCCRAC 4 cut(s) 94, 177, 523, 654
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 2 cut(s) 54, 774
MslI CAYNNNNRTG 1 cut(s) 765
MspA1I CMGCKG 2 cut(s) 28, 348
MspI CCGG 2 cut(s) 5, 461
MunI CAATTG 2 cut(s) 167, 434
MvnI CGCG 1 cut(s) 779
MwoI GCNNNNNNNGC 1 cut(s) 10
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 3 cut(s) 15, 376, 472
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 139, 389, 469, 511, 764
NlaIV GGNNCC 1 cut(s) 621
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 600
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 3 cut(s) 210, 312, 347
PpuMI RGGWCCY 1 cut(s) 492
PsiI TTATAA 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 621
PspPI GGNCC 2 cut(s) 23, 492
PspPPI RGGWCCY 1 cut(s) 492
PstI CTGCAG 1 cut(s) 213
PsuI RGATCY 2 cut(s) 376, 472
RsaI GTAC 2 cut(s) 32, 432
RsaNI GTAC 2 cut(s) 31, 431
RseI CAYNNNNRTG 1 cut(s) 765
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 602
SaqAI TTAA 2 cut(s) 54, 774
SatI GCNGC 3 cut(s) 209, 311, 346
Sau3AI GATC 3 cut(s) 15, 376, 472
Sau96I GGNCC 2 cut(s) 23, 492
SduI GDGCHC 2 cut(s) 237, 614
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 1 cut(s) 209
SinI GGWCC 2 cut(s) 23, 492
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 203, 624
SmoI CTYRAG 2 cut(s) 203, 624
Sse9I AATT 3 cut(s) 167, 434, 565
SsiI CCGC 3 cut(s) 26, 348, 779
SspMI CTAG 3 cut(s) 161, 249, 560
SstI GAGCTC 1 cut(s) 237
StyI CCWWGG 2 cut(s) 615, 742
TaaI ACNGT 1 cut(s) 487
TaqI TCGA 2 cut(s) 594, 603
TasI AATT 3 cut(s) 167, 434, 565
TatI WGTACW 2 cut(s) 30, 430
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 54, 774
Tru9I TTAA 2 cut(s) 54, 774
TscAI CASTG 1 cut(s) 137
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 3 cut(s) 208, 310, 345
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 2 cut(s) 152, 663
TspRI CASTG 1 cut(s) 137
VpaK11BI GGWCC 2 cut(s) 23, 492
XceI RCATGY 1 cut(s) 389
XmiI GTMKAC 2 cut(s) 400, 603
XspI CTAG 3 cut(s) 161, 249, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.