Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000408

Physical Location & Seq

Genomic Coordinates Forward (+)

24627955 .. 24633536

Gene Structure 5582 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1278700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGGCACTAGAGTTTATTGGCGGAACACTGTTTACGCTGCTCTACGACGGCGTTAAGCAAGCGATGAGCAAGAGCAGCACCTTTAAATCCCTTCTGGGAGACCTCAAATCTACACTAGACTCCCTAGTACCGAGAGACATCCAACAAATTGAAGAACATAATGTGGAATTGGGTCTCCCCAACGACGAAATCGAAAGTCTTAAATTGAAGATGGAGGAAGGCGTAAAGCTGGTTGTAAAGTTATCGAATTTTCGAATGTGGAACTATTGCTGTCTGTATGATTATACTGACCAAATTGTTGAGTTGGATAGGGCTCTCAAAAGATTGCTGCAAAAATTGAAGATGCAGGAAACAAGGGATGTGAAAGAGGTTTTATTGTTGTCGAGGGAAAACCGAGATAAATTGGATGAAGTGAACAGAAGACTGTTGGATATACAGAAATTACTGCAGCAGAGAGCTGGGGAAGGAGGGAGTGGCTTGCAGAATTTGGTTTCCGGAAATAGTACAGAAACCGCTCCGCCGGAGCAAACTCAAGGGAATAGTGGGGAACAAGTGACATCTTTTGATGGAGGAGCTTCTCTAGAAGCAGTGTTTTTGGCTTTGTTTGACGCTGTTATAGGAGCAAAGGACAAGACTGTGATGTTTAAACCACTATTTGAAAATCTTATATCCACCCTAGAATCTTTAAAACCATTGATTGAAGAGGTAACAAAACATAAGAAGATATTAGATCGCACAGAGGAGCGACTCGAAGATTTTAGAATACAAATGGAGAATGGTGTAGAGCTCATAGACAAGTGCGCCAAGGATCACTTGTGGGTCAGCTGCAAACAGTATAAATACACTGACAGGCTTCTCAGATTGGACGAATCGCTTCAAATACTGTTAAATATACTGAAAGTGCAAGTAGCAAGAGATGTGAAGCAGACCTCTGCTTCAGTGCAACGTATAGAGGCCGTGATCAAGCAAATTGAAGAAAGTGCTGTGCTTCAAGATCAAATTCAAACTAGAGCTCGGAATGCATTACCTGAACCTTTATCACATACAGTTGGATTAGATATGCTGAATGTGCGGGGAACAAGGGATGTGAAGGAGGCATTGCTTTCTGTCAGGAATAAAATAGTGGTTGCGGAGAAAATCGAAGGAAGTGGTGTGGTACAGAACCTAAGTGAAATTGAAGGATTGCGTTCAAGTAGCAAACTGTCGACTATCTCGGTGCCTTCAACTCTTCGGACAGAGGGTGAGATCGTGCAGTCCTCCAATGTGAAGAAATTCGGATTTAATGAACTGAAAACAGCCACCAGGAACTTCCATTCTGATTGTATTTTGGGTGAAGGACATTCAGGTTCTGTCTTTAAGGGGTGGGTTGATGAGAATTCACTAACAGCTGCCAAGCCTGGTACTGGCATGGTTGTTGCTGTGCAAAGGCTTAACCAAGAAGATCTGCTGGGTCACAAAGAATGGTTGGCGGAAATCAACTACCTTGGGCAGCTGCATCATGAAAATCTTGTGAGGTTGATTGGTTACTGCTTAGAGGATGACCACCGACTTTTGGTGTATGAATTTATGCCTCGCGGAAGCTTGGATAGACATCTATTTAACAAGTCTTCTTTCTTTCAACCACTTCCGTGGAACCTTCGTATGAGGATTGCCCTTGGTGCTGCTAAGGGTCTAGCATTTCTTAGCAGTGATCTGCAAGTGATTTATCGTGACTTCAAAACTTCTGATATCCTGCTGGATTCGATGAACAATGCTAAACTTTCAAATTTTGGTCTGGCCTGGGGTTGGCCAGCCAGTGATAAAACCCATGTCTCATCAAGGGTCTTGGGGACATATGGGTATGCAGCTCCTGAGTATATAGCCACAGGTCAATTCACTGCCAAAATTAATGTGTATAGTTTTGGAGTTGTTCTGCTCGAAATGGTGTCTGGCAGACCAGTTGTGGACAAGAATCGGCCATCCGGAGAACACAATTTAGTTGAATGGGCCAAGCCTTACCTTGCCAGCAAACGCAAAGTTTCCAAAATTTTTGATGCTCGTATTGAAGGACAGTACTCTTTGAGTGACGCTCTCAAAGTAGTTAATCTAGCAATTCACTGCTTAGCAGCAGACCCCAAGTCTAGGCCAAACATGAACGACGTGGTAATAACATTGGAGCAGCTTCAGAAAAGTGAACCTTCCCAAGTGCCGGCTGATCGTTCGAAGGGTCCAACCGGTCTGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009909 GO:0009910 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0060089 GO:0060860 GO:0060862 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

742

Amino Acids

83.02

Weight (kDa)

6.45

Isoelectric Point (pI)

38.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	9 - 137	1.2e-20	Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8	PF05659	190 - 318	1.7e-26	Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase	PF00069	439 - 715	8.7e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	441 - 716	1.9e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000408)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica	MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica	Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis	pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis	RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata	RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora	Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii	Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa	Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha	Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana	Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1216
AccBSI	CCGCTC	1 cut(s)	515
AccI	GTMKAC	1 cut(s)	1205
AccII	CGCG	1 cut(s)	1575
AccIII	TCCGGA	2 cut(s)	494, 1961
AciI	CCGC	7 cut(s)	21, 513, 518, 1072, 1130, 1469, 1575
AclWI	GGATC	1 cut(s)	816
AcoI	YGGCCR	2 cut(s)	1787, 1955
AcsI	RAATTY	8 cut(s)	247, 484, 999, 1271, 1375, 1562, 1765, 2025
AcuI	CTGAAG	2 cut(s)	921, 2147
AfaI	GTAC	5 cut(s)	129, 505, 1158, 1402, 2054
AfiI	CCNNNNNNNGG	5 cut(s)	1403, 1552, 1785, 2121, 2188
AgeI	ACCGGT	1 cut(s)	2213
AhdI	GACNNNNNGTC	1 cut(s)	2116
AjiI	CACGTC	1 cut(s)	2140
AjnI	CCWGG	3 cut(s)	1301, 1396, 1778
AleI	CACNNNNGTG	1 cut(s)	1627
Alw21I	GWGCWC	2 cut(s)	789, 1015
Alw26I	GTCTC	4 cut(s)	93, 129, 179, 1816
AlwI	GGATC	1 cut(s)	816
AlwNI	CAGNNNCTG	2 cut(s)	1349, 1850
Aor13HI	TCCGGA	2 cut(s)	494, 1961
AoxI	GGCC	6 cut(s)	954, 1776, 1787, 1955, 1986, 2123
ApoI	RAATTY	8 cut(s)	247, 484, 999, 1271, 1375, 1562, 1765, 2025
AseI	ATTAAT	1 cut(s)	1887
AsiGI	ACCGGT	1 cut(s)	2213
Asp700I	GAANNNNTTC	2 cut(s)	873, 1271
AspLEI	GCGC	1 cut(s)	803
AspS9I	GGNCC	2 cut(s)	1986, 2207
AsuHPI	GGTGA	2 cut(s)	1253, 1343
AsuII	TTCGAA	2 cut(s)	253, 2201
AvaII	GGWCC	1 cut(s)	2207
BalI	TGGCCA	1 cut(s)	1789
BanI	GGYRCC	1 cut(s)	1216
BanII	GRGCYC	3 cut(s)	316, 789, 1015
BbsI	GAAGAC	2 cut(s)	427, 1599
Bbv12I	GWGCWC	2 cut(s)	789, 1015
BccI	CCATC	3 cut(s)	205, 560, 1966
BceAI	ACGGC	2 cut(s)	64, 941
BciT130I	CCWGG	3 cut(s)	1303, 1398, 1780
BclI	TGATCA	1 cut(s)	960
BcoDI	GTCTC	4 cut(s)	93, 129, 179, 1816
BfaI	CTAG	9 cut(s)	8, 116, 125, 581, 677, 1008, 1673, 2087, 2121
BfmI	CTRYAG	1 cut(s)	446
BglII	AGATCT	1 cut(s)	1441
BlpI	GCTNAGC	1 cut(s)	2101
BmcAI	AGTACT	1 cut(s)	2054
Bme1390I	CCNGG	3 cut(s)	1303, 1398, 1780
Bme18I	GGWCC	1 cut(s)	2207
BmeRI	GACNNNNNGTC	1 cut(s)	2116
BmgBI	CACGTC	1 cut(s)	2140
BmgT120I	GGNCC	2 cut(s)	1986, 2207
BmiI	GGNNCC	3 cut(s)	1218, 1634, 2208
BmrFI	CCNGG	3 cut(s)	1303, 1398, 1780
BmsI	GCATC	3 cut(s)	333, 1504, 2023
BpiI	GAAGAC	2 cut(s)	427, 1599
BplI	GAGNNNNNCTC	2 cut(s)	2053, 2085
Bpu10I	CCTNAGC	1 cut(s)	1665
Bpu1102I	GCTNAGC	1 cut(s)	2101
Bpu14I	TTCGAA	2 cut(s)	253, 2201
BpuEI	CTTGAG	1 cut(s)	516
BsaBI	GATNNNNATC	1 cut(s)	1590
BsaI	GGTCTC	2 cut(s)	93, 179
BsaJI	CCNNGG	5 cut(s)	804, 1483, 1628, 1654, 1779
BsaWI	WCCGGW	3 cut(s)	494, 1961, 2213
Bsc4I	CCNNNNNNNGG	5 cut(s)	1403, 1552, 1785, 2121, 2188
Bse118I	RCCGGY	2 cut(s)	2188, 2213
Bse1I	ACTGG	3 cut(s)	1408, 1794, 1937
Bse3DI	GCAATG	1 cut(s)	1097
Bse8I	GATNNNNATC	1 cut(s)	1590
BseAI	TCCGGA	2 cut(s)	494, 1961
BseBI	CCWGG	3 cut(s)	1303, 1398, 1780
BseDI	CCNNGG	5 cut(s)	804, 1483, 1628, 1654, 1779
BseGI	GGATG	6 cut(s)	138, 364, 412, 1090, 1543, 1958
BseJI	GATNNNNATC	1 cut(s)	1590
BseLI	CCNNNNNNNGG	5 cut(s)	1403, 1552, 1785, 2121, 2188
BseMI	GCAATG	1 cut(s)	1097
BseMII	CTCAG	2 cut(s)	871, 1842
BseNI	ACTGG	3 cut(s)	1408, 1794, 1937
BseRI	GAGGAG	2 cut(s)	585, 755
BseYI	CCCAGC	2 cut(s)	458, 1447
BsgI	GTGCAG	1 cut(s)	1271
Bsh1236I	CGCG	1 cut(s)	1575
BshFI	GGCC	6 cut(s)	956, 1778, 1789, 1957, 1988, 2125
BshNI	GGYRCC	1 cut(s)	1216
BshTI	ACCGGT	1 cut(s)	2213
BsiHKAI	GWGCWC	2 cut(s)	789, 1015
BsiSI	CCGG	5 cut(s)	495, 521, 1962, 2189, 2214
BslFI	GGGAC	1 cut(s)	1843
BslI	CCNNNNNNNGG	5 cut(s)	1403, 1552, 1785, 2121, 2188
BsmAI	GTCTC	4 cut(s)	93, 129, 179, 1816
BsmFI	GGGAC	1 cut(s)	1843
BsmI	GAATGC	1 cut(s)	1024
BsnI	GGCC	6 cut(s)	956, 1778, 1789, 1957, 1988, 2125
Bso31I	GGTCTC	2 cut(s)	93, 179
Bsp119I	TTCGAA	2 cut(s)	253, 2201
Bsp1286I	GDGCHC	3 cut(s)	316, 789, 1015
Bsp13I	TCCGGA	2 cut(s)	494, 1961
Bsp143I	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
Bsp1720I	GCTNAGC	1 cut(s)	2101
BspACI	CCGC	7 cut(s)	21, 513, 518, 1072, 1130, 1469, 1575
BspANI	GGCC	6 cut(s)	956, 1778, 1789, 1957, 1988, 2125
BspCNI	CTCAG	2 cut(s)	870, 1843
BspEI	TCCGGA	2 cut(s)	494, 1961
BspFNI	CGCG	1 cut(s)	1575
BspHI	TCATGA	1 cut(s)	1498
BspLI	GGNNCC	3 cut(s)	1218, 1634, 2208
BspMAI	CTGCAG	1 cut(s)	450
BspPI	GGATC	1 cut(s)	816
BspT104I	TTCGAA	2 cut(s)	253, 2201
BspT107I	GGYRCC	1 cut(s)	1216
BspTNI	GGTCTC	2 cut(s)	93, 179
BsrBI	CCGCTC	1 cut(s)	515
BsrDI	GCAATG	1 cut(s)	1097
BsrFI	RCCGGY	2 cut(s)	2188, 2213
BsrI	ACTGG	3 cut(s)	1408, 1794, 1937
BssAI	RCCGGY	2 cut(s)	2188, 2213
BssECI	CCNNGG	5 cut(s)	804, 1483, 1628, 1654, 1779
BssMI	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
BssT1I	CCWWGG	3 cut(s)	804, 1483, 1654
Bst2UI	CCWGG	3 cut(s)	1303, 1398, 1780
Bst4CI	ACNGT	8 cut(s)	30, 426, 637, 834, 885, 1048, 1203, 2052
Bst6I	CTCTTC	2 cut(s)	696, 1233
BstBI	TTCGAA	2 cut(s)	253, 2201
BstC8I	GCNNGC	5 cut(s)	60, 479, 1791, 2005, 2190
BstDEI	CTNAG	7 cut(s)	857, 1166, 1531, 1665, 1682, 1851, 2101
BstDSI	CCRYGG	1 cut(s)	1628
BstF5I	GGATG	6 cut(s)	138, 364, 412, 1090, 1543, 1958
BstFNI	CGCG	1 cut(s)	1575
BstHHI	GCGC	1 cut(s)	803
BstKTI	GATC	8 cut(s)	733, 811, 963, 997, 1248, 1444, 1693, 2197
BstMAI	GTCTC	4 cut(s)	93, 129, 179, 1816
BstMBI	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
BstMWI	GCNNNNNNNGC	4 cut(s)	75, 1019, 1069, 1658
BstNI	CCWGG	3 cut(s)	1303, 1398, 1780
BstSCI	CCNGG	3 cut(s)	1301, 1396, 1778
BstSFI	CTRYAG	1 cut(s)	446
BstUI	CGCG	1 cut(s)	1575
BstV2I	GAAGAC	2 cut(s)	427, 1599
BstX2I	RGATCY	1 cut(s)	1441
BstXI	CCANNNNNNTGG	1 cut(s)	1629
BstYI	RGATCY	1 cut(s)	1441
BsuRI	GGCC	6 cut(s)	956, 1778, 1789, 1957, 1988, 2125
BtgI	CCRYGG	1 cut(s)	1628
BtgZI	GCGATG	1 cut(s)	77
BtrI	CACGTC	1 cut(s)	2140
BtsCI	GGATG	6 cut(s)	138, 364, 412, 1090, 1543, 1958
BtsI	GCAGTG	4 cut(s)	594, 1693, 1875, 2095
BtsIMutI	CAGTG	8 cut(s)	26, 594, 843, 945, 1693, 1801, 1875, 2095
Cac8I	GCNNGC	5 cut(s)	60, 479, 1791, 2005, 2190
CaiI	CAGNNNCTG	2 cut(s)	1349, 1850
CciI	TCATGA	1 cut(s)	1498
CfoI	GCGC	1 cut(s)	803
Cfr10I	RCCGGY	2 cut(s)	2188, 2213
Cfr13I	GGNCC	2 cut(s)	1986, 2207
CseI	GACGC	2 cut(s)	617, 2075
Csp6I	GTAC	5 cut(s)	128, 504, 1157, 1401, 2053
CspAI	ACCGGT	1 cut(s)	2213
CviAII	CATG	5 cut(s)	1408, 1499, 1808, 2131, 2223
CviQI	GTAC	5 cut(s)	128, 504, 1157, 1401, 2053
DdeI	CTNAG	7 cut(s)	857, 1166, 1531, 1665, 1682, 1851, 2101
DpnI	GATC	8 cut(s)	732, 810, 962, 996, 1247, 1443, 1692, 2196
DpnII	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
DraI	TTTAAA	3 cut(s)	85, 646, 687
DriI	GACNNNNNGTC	1 cut(s)	2116
EaeI	YGGCCR	2 cut(s)	1787, 1955
Eam1104I	CTCTTC	2 cut(s)	696, 1233
Eam1105I	GACNNNNNGTC	1 cut(s)	2116
EarI	CTCTTC	2 cut(s)	696, 1233
EciI	GGCGGA	3 cut(s)	36, 507, 1484
Ecl136II	GAGCTC	2 cut(s)	787, 1013
Eco130I	CCWWGG	3 cut(s)	804, 1483, 1654
Eco24I	GRGCYC	3 cut(s)	316, 789, 1015
Eco31I	GGTCTC	2 cut(s)	93, 179
Eco32I	GATATC	1 cut(s)	1729
Eco47I	GGWCC	1 cut(s)	2207
Eco53kI	GAGCTC	2 cut(s)	787, 1013
Eco57I	CTGAAG	2 cut(s)	921, 2147
EcoICRI	GAGCTC	2 cut(s)	787, 1013
EcoRI	GAATTC	1 cut(s)	1375
EcoRII	CCWGG	3 cut(s)	1301, 1396, 1778
EcoRV	GATATC	1 cut(s)	1729
EcoT14I	CCWWGG	3 cut(s)	804, 1483, 1654
EcoT22I	ATGCAT	1 cut(s)	1024
EcoT38I	GRGCYC	3 cut(s)	316, 789, 1015
ErhI	CCWWGG	3 cut(s)	804, 1483, 1654
FaeI	CATG	5 cut(s)	1411, 1502, 1811, 2134, 2226
FalI	AAGNNNNNCTT	4 cut(s)	797, 829, 2161, 2193
FaqI	GGGAC	1 cut(s)	1843
FatI	CATG	5 cut(s)	1407, 1498, 1807, 2130, 2222
FauI	CCCGC	1 cut(s)	1065
FauNDI	CATATG	1 cut(s)	1834
FbaI	TGATCA	1 cut(s)	960
FblI	GTMKAC	1 cut(s)	1205
FokI	GGATG	6 cut(s)	125, 371, 419, 1097, 1550, 1945
FriOI	GRGCYC	3 cut(s)	316, 789, 1015
FspBI	CTAG	9 cut(s)	8, 116, 125, 581, 677, 1008, 1673, 2087, 2121
GlaI	GCGC	1 cut(s)	802
GsaI	CCCAGC	2 cut(s)	462, 1451
HaeIII	GGCC	6 cut(s)	956, 1778, 1789, 1957, 1988, 2125
HapII	CCGG	5 cut(s)	495, 521, 1962, 2189, 2214
HgaI	GACGC	2 cut(s)	617, 2075
HhaI	GCGC	1 cut(s)	803
Hin1II	CATG	5 cut(s)	1411, 1502, 1811, 2134, 2226
Hin6I	GCGC	1 cut(s)	801
HinP1I	GCGC	1 cut(s)	801
HincII	GTYRAC	1 cut(s)	1206
HindII	GTYRAC	1 cut(s)	1206
HindIII	AAGCTT	1 cut(s)	1579
HinfI	GANTC	6 cut(s)	119, 680, 747, 869, 1739, 1951
HpaII	CCGG	5 cut(s)	495, 521, 1962, 2189, 2214
HphI	GGTGA	2 cut(s)	1253, 1343
Hpy166II	GTNNAC	5 cut(s)	33, 415, 1206, 1945, 2174
Hpy188I	TCNGA	8 cut(s)	860, 1017, 1233, 1277, 1318, 1726, 2166, 2220
Hpy188III	TCNNGA	8 cut(s)	495, 581, 992, 1111, 1499, 1709, 1850, 1962
Hpy8I	GTNNAC	5 cut(s)	33, 415, 1206, 1945, 2174
Hpy99I	CGWCG	3 cut(s)	50, 188, 2141
HpyCH4III	ACNGT	8 cut(s)	30, 426, 637, 834, 885, 1048, 1203, 2052
HpyCH4IV	ACGT	2 cut(s)	946, 2139
HpyF10VI	GCNNNNNNNGC	4 cut(s)	75, 1019, 1069, 1658
HpyF3I	CTNAG	7 cut(s)	857, 1166, 1531, 1665, 1682, 1851, 2101
HpySE526I	ACGT	2 cut(s)	946, 2139
Hsp92II	CATG	5 cut(s)	1411, 1502, 1811, 2134, 2226
HspAI	GCGC	1 cut(s)	801
Kpn2I	TCCGGA	2 cut(s)	494, 1961
KroI	GCCGGC	1 cut(s)	2188
KroNI	GCCGGC	1 cut(s)	2190
Ksp22I	TGATCA	1 cut(s)	960
Kzo9I	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
LmnI	GCTCC	7 cut(s)	520, 523, 572, 620, 742, 1852, 2155
LweI	GCATC	3 cut(s)	333, 1504, 2023
MaeI	CTAG	9 cut(s)	8, 116, 125, 581, 677, 1008, 1673, 2087, 2121
MaeII	ACGT	2 cut(s)	946, 2139
MaeIII	GTNAC	6 cut(s)	553, 706, 1451, 1523, 1709, 2063
MalI	GATC	8 cut(s)	732, 810, 962, 996, 1247, 1443, 1692, 2196
MbiI	CCGCTC	1 cut(s)	515
MboI	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
MflI	RGATCY	1 cut(s)	1441
MhlI	GDGCHC	3 cut(s)	316, 789, 1015
MlsI	TGGCCA	1 cut(s)	1789
MluNI	TGGCCA	1 cut(s)	1789
MlyI	GAGTC	2 cut(s)	113, 741
MmeI	TCCRAC	4 cut(s)	166, 285, 408, 1030
Mox20I	TGGCCA	1 cut(s)	1789
Mph1103I	ATGCAT	1 cut(s)	1024
MroI	TCCGGA	2 cut(s)	494, 1961
MroNI	GCCGGC	1 cut(s)	2188
MroXI	GAANNNNTTC	2 cut(s)	873, 1271
MscI	TGGCCA	1 cut(s)	1789
MslI	CAYNNNNRTG	1 cut(s)	1627
Msp20I	TGGCCA	1 cut(s)	1789
MspA1I	CMGCKG	3 cut(s)	825, 1388, 1492
MspI	CCGG	5 cut(s)	495, 521, 1962, 2189, 2214
MspR9I	CCNGG	3 cut(s)	1303, 1398, 1780
MssI	GTTTAAAC	1 cut(s)	646
Mva1269I	GAATGC	1 cut(s)	1024
MvaI	CCWGG	3 cut(s)	1303, 1398, 1780
MvnI	CGCG	1 cut(s)	1575
MwoI	GCNNNNNNNGC	4 cut(s)	75, 1019, 1069, 1658
NaeI	GCCGGC	1 cut(s)	2190
NdeI	CATATG	1 cut(s)	1834
NdeII	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
NgoMIV	GCCGGC	1 cut(s)	2188
NlaIII	CATG	5 cut(s)	1411, 1502, 1811, 2134, 2226
NlaIV	GGNNCC	3 cut(s)	1218, 1634, 2208
NmuCI	GTSAC	4 cut(s)	553, 1451, 1709, 2063
NsiI	ATGCAT	1 cut(s)	1024
NspV	TTCGAA	2 cut(s)	253, 2201
OliI	CACNNNNGTG	1 cut(s)	1627
PagI	TCATGA	1 cut(s)	1498
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PctI	GAATGC	1 cut(s)	1024
PdiI	GCCGGC	1 cut(s)	2190
PdmI	GAANNNNTTC	2 cut(s)	873, 1271
PfeI	GAWTC	4 cut(s)	680, 869, 1739, 1951
PinAI	ACCGGT	1 cut(s)	2213
PleI	GAGTC	2 cut(s)	113, 741
PmeI	GTTTAAAC	1 cut(s)	646
PpsI	GAGTC	2 cut(s)	113, 741
PshBI	ATTAAT	1 cut(s)	1887
Psp124BI	GAGCTC	2 cut(s)	789, 1015
Psp6I	CCWGG	3 cut(s)	1301, 1396, 1778
PspFI	CCCAGC	2 cut(s)	458, 1447
PspGI	CCWGG	3 cut(s)	1301, 1396, 1778
PspN4I	GGNNCC	3 cut(s)	1218, 1634, 2208
PspPI	GGNCC	2 cut(s)	1986, 2207
PsrI	GAACNNNNNNTAC	2 cut(s)	16, 48
PstI	CTGCAG	1 cut(s)	450
PstNI	CAGNNNCTG	2 cut(s)	1349, 1850
PsuI	RGATCY	1 cut(s)	1441
PvuII	CAGCTG	3 cut(s)	825, 1388, 1492
RsaI	GTAC	5 cut(s)	129, 505, 1158, 1402, 2054
RsaNI	GTAC	5 cut(s)	128, 504, 1157, 1401, 2053
RseI	CAYNNNNRTG	1 cut(s)	1627
SacI	GAGCTC	2 cut(s)	789, 1015
SalI	GTCGAC	1 cut(s)	1204
Sau3AI	GATC	8 cut(s)	730, 808, 960, 994, 1245, 1441, 1690, 2194
Sau96I	GGNCC	2 cut(s)	1986, 2207
ScaI	AGTACT	1 cut(s)	2054
SchI	GAGTC	2 cut(s)	113, 741
ScrFI	CCNGG	3 cut(s)	1303, 1398, 1780
SduI	GDGCHC	3 cut(s)	316, 789, 1015
SfaNI	GCATC	3 cut(s)	333, 1504, 2023
SfcI	CTRYAG	1 cut(s)	446
SfuI	TTCGAA	2 cut(s)	253, 2201
SinI	GGWCC	1 cut(s)	2207
SmiMI	CAYNNNNRTG	1 cut(s)	1627
SmlI	CTYRAG	1 cut(s)	531
SmoI	CTYRAG	1 cut(s)	531
SsiI	CCGC	7 cut(s)	21, 513, 518, 1072, 1130, 1469, 1575
SspMI	CTAG	9 cut(s)	8, 116, 125, 581, 677, 1008, 1673, 2087, 2121
SstI	GAGCTC	2 cut(s)	789, 1015
StyD4I	CCNGG	3 cut(s)	1301, 1396, 1778
StyI	CCWWGG	3 cut(s)	804, 1483, 1654
TaaI	ACNGT	8 cut(s)	30, 426, 637, 834, 885, 1048, 1203, 2052
TaiI	ACGT	2 cut(s)	949, 2142
TatI	WGTACW	2 cut(s)	503, 2052
TfiI	GAWTC	4 cut(s)	680, 869, 1739, 1951
TscAI	CASTG	8 cut(s)	33, 594, 850, 945, 1693, 1801, 1882, 2102
TseFI	GTSAC	4 cut(s)	553, 1451, 1709, 2063
Tsp45I	GTSAC	4 cut(s)	553, 1451, 1709, 2063
TspDTI	ATGAA	6 cut(s)	423, 1299, 1515, 1575, 1760, 2147
TspGWI	ACGGA	1 cut(s)	1617
TspRI	CASTG	8 cut(s)	33, 594, 850, 945, 1693, 1801, 1882, 2102
VpaK11BI	GGWCC	1 cut(s)	2207
VspI	ATTAAT	1 cut(s)	1887
XapI	RAATTY	8 cut(s)	247, 484, 999, 1271, 1375, 1562, 1765, 2025
XbaI	TCTAGA	1 cut(s)	580
XmiI	GTMKAC	1 cut(s)	1205
XmnI	GAANNNNTTC	2 cut(s)	873, 1271
XspI	CTAG	9 cut(s)	8, 116, 125, 581, 677, 1008, 1673, 2087, 2121
ZrmI	AGTACT	1 cut(s)	2054
Zsp2I	ATGCAT	1 cut(s)	1024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.