RLG00000004828

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000408
Physical Location & Seq
Reverse (-)
62853006 .. 62856774
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004828

Sequence Viewer

Length: 2736 bp
ATGACGTCTCTGACTCTAACCTTTCTGGGGGGAGCCCCTTTTCAATTGCTGTATGAAGGCGTTAAGATAGCTATTCGCAAAAGTAACAAGTTTAGAACTCACCTTGAAAATCTGGAAATGACGCTAGGCTGTTTGCAACCATGGATCATTCAACAGATAAGAGCAAATAATGTGCAACTGAATCTCCCAAATAATTTGATACAAGATCTTGAAATAAAAATGGAGGGGGGCAAAGAGCTGGTTTACAAGTTATCAAGGCTTGGCGTTTGGAACATCTGTGTCTGGGGAAATTGCTACAATTGTATAAAGCCTAATTACGCAGACCAACTTGCTGAATTGGACAGATCTCTTAGATTACTATTAGAAATACTAAAGCTGGAGGAAATGAGGAATGTTCAGGAGAACTTACTATTGGCGAGGAACCACAGTGACAAACAAGATGATTTGGACAAAAAAATGTCCGAGTTATTGAAAGTGCATCAGGAAGTTCTGAAAGCGCAGAAAGAAATGTTTGATATGATGAGAGTGCAGCATGAGGCTGTACATGAGGCAATGCAGAGAATTAGAGCTGATGCTGTGCAAAATGTGCGAGGAGAAACTGCCCCTGCTCCAGCTCCAGCTCTAAGAATAGTATTTTGGGTGCTGTATGATGTTGTTATACAAGTGAAGGTCAAGAATACAATGTGTAAACGCCTCCTTCAAGAATTCAAATCCACGCTCGACTTCTTAAAACCACTGATAGAAGAAATAGCAGCAAGTAACAAACTATTGCATCTTCCAGAGCAGGAACAAGAGAAGTTTATTATTCAAATGAGGGAGGGTGTAGAGCTCATTGACAAGTGCTCCAAGGTTAGCAAGTGGCATAGTTACAAAAAGTATGCATGCACCAACAAACTTCTTAAATTAGATGAATCTCTCCGGGGGCTATTCCATAAAATGAGTATGCAGATTGCAATCCATTTGAAGGAAGGTTTGATTTCAATAAGTAGTATGGAGGCAATGATCAAGAAAATTGAAGAGAGTGGTGTGATACAAAATCAAATTCATGTTGTACTCACTAAACCACAACCTCCACTGCATGAAGCTGGATTGGATGTTCTGGATACGCATGGAAGTAAGGATGTGGAGGGAACAAGAGATGATTTAAATGAGAAAGAGGTTGGCGTGAAGCAAATTGGAAGAGATGGTACAAGACAGAATGAAAAAGAGATTGAAGGTTTGAGTGAAGTACCTGAAACTTCATTGCCTAAAACTACATTGGATGTGCAAGGAACAAAGGATATAAATGAGTCACTAGATTCAGAAAGTGTACATATAGCGCAGACCAAGCAAAGTCAAGGGACAAGGATGGTTGAGGCTCCAGTTGCTGAGTTATATAAATTTCCATTCCTTATGGTTGAATCGGATGTGTATTTGAGGGAATTAAAGAAGAAGATTTTTGAGGATAAGGTGGCGATGCTTGTGATTACAGGTCCAGCAGGATGCGGGAAAACCACACTGGCAAGAATGTTTTGTGAAGATCAGGAGGTCAAAATGAAATTCAAGAATATTTTCTTTGTCCATGTTTCAGAAAATCCCAGCTTGGATTTTATTGTCCAACAACTATATGAACAAAAGGATTCAGTGGTACTTGAGCTGCAAGAAGATGCAGTTAACTGGCTGCAACAGTTTCTAAAGGAAACAGGAGGAGATCCTTCATTGTTGGTCTTGGATGATGTTTGGTCCGGATCAGAATCTCTTCTTGATAAGTTTGATGAATTAAGAACTTCAATGCACAAGATTTTGGTGACATCAAGATCTGAATTTCCACGATTTGATTGTTATCTAGAAAACCTAAAATCACTGGATGATGAAGAGGAAATGGCTCACCTTAAGGGAAAGGTTGTGGCGGCCGTGAGAGCTACAGGGGACTACCCAGAAAGAGTGGGCAAACCTATATGTGAGTATTATTTAAAGACTGGGACCTGTAAATTTGGTCCGTCCTGCAAGTTCCATCATCCGAAACGGGGAATTAGATACTTGAGCTCTTTGTATGGACTGACACAGCTATCTTCTCCCACCCATGCCCTTGCAAGACCTTATACCTATGTCGTACCATCATCTGTTGGCCCTTCTAGCAGCAGCCCGAATGAACAAGCCTTCCCAGAGAGGCCTGGAGTAGCTGAATGCCAGTATTACCTGAGAACTGGAGACTGTAAATTTGGACCCTCATGTCGGTACCACCATCCTCGAGATCGGATTGCACCAAGGACAAATTGTCTCCTCAGTCCAGTTGGTCTTCTTGGGAATTCAGGCTCTAATGCTTGGCCTTCATTCAGTCAAATGTCATTGTCAATGGAAGATTTTCAGACGTCTTTGGATTTGAAATTGAATGCTCAGCATTGCCTTCTCCAGACACCAATGTCACCTTGTGTATCCATAAGTCGAAGAATAAGCAGCAGTGGCAAGTCCAGTCAGTCAGATGTAGAGGATAAGAGTGAGCTTGATGAGATTCAAAGCCAAATTTCAGGTGTCACTGTTCTGGAGTCGCTACATGATCCACTTGAAAGTAAAGATCAGTGTCCAATGAATGTGAACAACACATTGGAATATGCAGCTTCAGGGACAACTCCGAATTCAGTTAACGCTGATGGTCAGTCTGTTGCTTGTAACAGCAGTAAGTGTATAAACTACTTCGTTCAGTTAGTCAATGTCAATAATTCTGGTCATTCAGAGCTAGCTCCACATGTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

912

Amino Acids

102.78

Weight (kDa)

5.98

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 11 - 137 9.3e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 206 - 336 2.5e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 472 - 601 1.1e-14 NB-ARC domain
AAA_22 PF13401 486 - 573 7.8e-07 AAA domain
zf-CCCH PF00642 644 - 668 9.2e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 719 - 744 4.1e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2211, 2401
AatII GACGTC 2 cut(s) 8, 2354
Acc65I GGTACC 1 cut(s) 2217
AccB1I GGYRCC 1 cut(s) 2217
AccIII TCCGGA 1 cut(s) 1724
AciI CCGC 2 cut(s) 1485, 1889
AclWI GGATC 4 cut(s) 152, 1685, 1735, 2531
AcoI YGGCCR 1 cut(s) 1890
AcuI CTGAAG 1 cut(s) 2583
AcyI GRCGYC 2 cut(s) 5, 2351
AdeI CACNNNGTG 1 cut(s) 2411
AfaI GTAC 8 cut(s) 543, 1053, 1189, 1230, 1311, 1629, 2094, 2219
AfiI CCNNNNNNNGG 4 cut(s) 27, 964, 2006, 2214
AflII CTTAAG 1 cut(s) 1871
AflIII ACRYGT 1 cut(s) 2725
AhdI GACNNNNNGTC 1 cut(s) 2256
AjnI CCWGG 1 cut(s) 2152
AjuI GAANNNNNNNTTGG 6 cut(s) 395, 427, 619, 651, 2567, 2599
AloI GAACNNNNNNTCC 4 cut(s) 1080, 1112, 2124, 2156
Alw21I GWGCWC 3 cut(s) 831, 845, 2027
Alw26I GTCTC 3 cut(s) 12, 2184, 2264
AlwI GGATC 4 cut(s) 152, 1685, 1735, 2531
AlwNI CAGNNNCTG 1 cut(s) 1367
Ama87I CYCGRG 1 cut(s) 2229
Aor13HI TCCGGA 1 cut(s) 1724
AoxI GGCC 4 cut(s) 1890, 2107, 2150, 2306
ApeKI GCWGC 8 cut(s) 529, 752, 1636, 1660, 2118, 2121, 2436, 2594
ArsI GACNNNNNNTTYG 2 cut(s) 2183, 2215
Asp700I GAANNNNTTC 3 cut(s) 1550, 1737, 2343
Asp718I GGTACC 1 cut(s) 2217
AspLEI GCGC 2 cut(s) 499, 1321
AspS9I GGNCC 6 cut(s) 1472, 1722, 1962, 1976, 2108, 2204
AsuC2I CCSGG 1 cut(s) 920
AsuHPI GGTGA 4 cut(s) 92, 1798, 1859, 2397
AsuNHI GCTAGC 1 cut(s) 2716
AvaI CYCGRG 1 cut(s) 2229
AvaII GGWCC 5 cut(s) 1472, 1722, 1962, 1976, 2204
BanI GGYRCC 1 cut(s) 2217
BanII GRGCYC 3 cut(s) 37, 831, 2027
BarI GAAGNNNNNNTAC 2 cut(s) 1171, 1203
BbsI GAAGAC 1 cut(s) 2270
Bbv12I GWGCWC 3 cut(s) 831, 845, 2027
BbvI GCAGC 8 cut(s) 541, 764, 1623, 1647, 2130, 2133, 2448, 2606
BccI CCATC 6 cut(s) 1178, 1342, 2001, 2104, 2232, 2624
BceAI ACGGC 1 cut(s) 1877
BciT130I CCWGG 1 cut(s) 2154
BciVI GTATCC 2 cut(s) 1096, 2425
BclI TGATCA 1 cut(s) 1002
BcnI CCSGG 1 cut(s) 920
BcoDI GTCTC 3 cut(s) 12, 2184, 2264
BfaI CTAG 5 cut(s) 125, 1295, 1826, 2115, 2717
BfmI CTRYAG 1 cut(s) 1902
BfrI CTTAAG 1 cut(s) 1871
BfuI GTATCC 2 cut(s) 1096, 2425
BglII AGATCT 3 cut(s) 205, 344, 1796
BisI GCNGC 9 cut(s) 530, 753, 1637, 1661, 1890, 2119, 2122, 2437, 2595
BlpI GCTNAGC 1 cut(s) 2376
BlsI GCNGC 9 cut(s) 531, 754, 1638, 1662, 1891, 2120, 2123, 2438, 2596
Bme1390I CCNGG 2 cut(s) 920, 2154
Bme18I GGWCC 5 cut(s) 1472, 1722, 1962, 1976, 2204
BmeRI GACNNNNNGTC 1 cut(s) 2256
BmeT110I CYCGRG 1 cut(s) 2229
BmgT120I GGNCC 6 cut(s) 1472, 1722, 1962, 1976, 2108, 2204
BmiI GGNNCC 6 cut(s) 34, 422, 1359, 1963, 2206, 2219
BmrFI CCNGG 2 cut(s) 920, 2154
BmrI ACTGGG 1 cut(s) 1968
BmsI GCATC 6 cut(s) 487, 562, 781, 1446, 1472, 1636
BmtI GCTAGC 1 cut(s) 2720
BmuI ACTGGG 1 cut(s) 1968
BpiI GAAGAC 1 cut(s) 2270
BpmI CTGGAG 8 cut(s) 398, 594, 600, 1344, 2175, 2208, 2375, 2543
Bpu1102I GCTNAGC 1 cut(s) 2376
BpuEI CTTGAG 2 cut(s) 1652, 2041
BpuMI CCSGG 1 cut(s) 920
BsaBI GATNNNNATC 3 cut(s) 953, 1732, 1821
BsaHI GRCGYC 2 cut(s) 5, 2351
BsaJI CCNNGG 4 cut(s) 140, 846, 919, 2246
BsaWI WCCGGW 1 cut(s) 1724
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 827, 857
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 964, 2006, 2214
Bse1I ACTGG 9 cut(s) 1361, 1503, 1661, 1848, 1963, 2170, 2191, 2270, 2451
Bse3DI GCAATG 4 cut(s) 558, 1005, 1241, 2380
Bse8I GATNNNNATC 3 cut(s) 953, 1732, 1821
BseAI TCCGGA 1 cut(s) 1724
BseBI CCWGG 1 cut(s) 2154
BseDI CCNNGG 4 cut(s) 140, 846, 919, 2246
BseJI GATNNNNATC 3 cut(s) 953, 1732, 1821
BseLI CCNNNNNNNGG 4 cut(s) 27, 964, 2006, 2214
BseMI GCAATG 4 cut(s) 558, 1005, 1241, 2380
BseMII CTCAG 4 cut(s) 1359, 2171, 2278, 2390
BseNI ACTGG 9 cut(s) 1361, 1503, 1661, 1848, 1963, 2170, 2191, 2270, 2451
BseRI GAGGAG 3 cut(s) 606, 1701, 2252
BseX3I CGGCCG 1 cut(s) 1890
BseXI GCAGC 8 cut(s) 541, 764, 1623, 1647, 2130, 2133, 2448, 2606
BseYI CCCAGC 1 cut(s) 1577
BsgI GTGCAG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 1893
BshFI GGCC 4 cut(s) 1892, 2109, 2152, 2308
BshNI GGYRCC 1 cut(s) 2217
BsiEI CGRYCG 1 cut(s) 1893
BsiHKAI GWGCWC 3 cut(s) 831, 845, 2027
BsiHKCI CYCGRG 1 cut(s) 2229
BsiSI CCGG 2 cut(s) 919, 1725
BslFI GGGAC 4 cut(s) 1354, 1922, 1975, 2617
BslI CCNNNNNNNGG 4 cut(s) 27, 964, 2006, 2214
BsmAI GTCTC 3 cut(s) 12, 2184, 2264
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 4 cut(s) 1354, 1922, 1975, 2617
BsmI GAATGC 2 cut(s) 2171, 2377
BsnI GGCC 4 cut(s) 1892, 2109, 2152, 2308
BsoBI CYCGRG 1 cut(s) 2229
Bsp1286I GDGCHC 4 cut(s) 37, 831, 845, 2027
Bsp13I TCCGGA 1 cut(s) 1724
Bsp1407I TGTACA 2 cut(s) 541, 1309
Bsp1720I GCTNAGC 1 cut(s) 2376
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 2 cut(s) 1485, 1889
BspANI GGCC 4 cut(s) 1892, 2109, 2152, 2308
BspCNI CTCAG 4 cut(s) 1360, 2172, 2277, 2389
BspEI TCCGGA 1 cut(s) 1724
BspLI GGNNCC 6 cut(s) 34, 422, 1359, 1963, 2206, 2219
BspOI GCTAGC 1 cut(s) 2720
BspPI GGATC 4 cut(s) 152, 1685, 1735, 2531
BspT107I GGYRCC 1 cut(s) 2217
BspTI CTTAAG 1 cut(s) 1871
BsrDI GCAATG 4 cut(s) 558, 1005, 1241, 2380
BsrGI TGTACA 2 cut(s) 541, 1309
BsrI ACTGG 9 cut(s) 1361, 1503, 1661, 1848, 1963, 2170, 2191, 2270, 2451
BssECI CCNNGG 4 cut(s) 140, 846, 919, 2246
BssNI GRCGYC 2 cut(s) 5, 2351
BssT1I CCWWGG 3 cut(s) 140, 846, 2246
Bst2UI CCWGG 1 cut(s) 2154
Bst4CI ACNGT 4 cut(s) 428, 1668, 2195, 2518
Bst6I CTCTTC 4 cut(s) 1011, 1174, 1743, 1848
BstACI GRCGYC 2 cut(s) 5, 2351
BstAFI CTTAAG 1 cut(s) 1871
BstAPI GCANNNNNTGC 1 cut(s) 586
BstAUI TGTACA 2 cut(s) 541, 1309
BstC8I GCNNGC 2 cut(s) 883, 2718
BstDEI CTNAG 6 cut(s) 350, 623, 1368, 2180, 2264, 2376
BstDSI CCRYGG 1 cut(s) 140
BstHHI GCGC 2 cut(s) 499, 1321
BstMAI GTCTC 3 cut(s) 12, 2184, 2264
BstMCI CGRYCG 1 cut(s) 1893
BstMWI GCNNNNNNNGC 6 cut(s) 586, 1327, 1364, 1898, 2115, 2442
BstNI CCWGG 1 cut(s) 2154
BstNSI RCATGY 2 cut(s) 885, 2729
BstSCI CCNGG 2 cut(s) 918, 2152
BstSFI CTRYAG 1 cut(s) 1902
BstV1I GCAGC 8 cut(s) 541, 764, 1623, 1647, 2130, 2133, 2448, 2606
BstV2I GAAGAC 1 cut(s) 2270
BstX2I RGATCY 4 cut(s) 205, 344, 1690, 1796
BstYI RGATCY 4 cut(s) 205, 344, 1690, 1796
BstZI CGGCCG 1 cut(s) 1890
BsuI GTATCC 2 cut(s) 1096, 2425
BsuRI GGCC 4 cut(s) 1892, 2109, 2152, 2308
BtgI CCRYGG 1 cut(s) 140
BtgZI GCGATG 1 cut(s) 1469
BtsI GCAGTG 2 cut(s) 1073, 2446
BtsIMutI CAGTG 9 cut(s) 433, 734, 1073, 1496, 1629, 1841, 2446, 2514, 2564
Cac8I GCNNGC 2 cut(s) 883, 2718
CaiI CAGNNNCTG 1 cut(s) 1367
CfoI GCGC 2 cut(s) 499, 1321
Cfr13I GGNCC 6 cut(s) 1472, 1722, 1962, 1976, 2108, 2204
CseI GACGC 1 cut(s) 130
Csp6I GTAC 8 cut(s) 542, 1052, 1188, 1229, 1310, 1628, 2093, 2218
CviQI GTAC 8 cut(s) 542, 1052, 1188, 1229, 1310, 1628, 2093, 2218
DdeI CTNAG 6 cut(s) 350, 623, 1368, 2180, 2264, 2376
DraI TTTAAA 2 cut(s) 1146, 1953
DraIII CACNNNGTG 1 cut(s) 2411
DrdI GACNNNNNNGTC 2 cut(s) 2211, 2401
DriI GACNNNNNGTC 1 cut(s) 2256
DseDI GACNNNNNNGTC 2 cut(s) 2211, 2401
EaeI YGGCCR 1 cut(s) 1890
EagI CGGCCG 1 cut(s) 1890
Eam1104I CTCTTC 4 cut(s) 1011, 1174, 1743, 1848
Eam1105I GACNNNNNGTC 1 cut(s) 2256
EarI CTCTTC 4 cut(s) 1011, 1174, 1743, 1848
Ecl136II GAGCTC 2 cut(s) 829, 2025
EclXI CGGCCG 1 cut(s) 1890
Eco130I CCWWGG 3 cut(s) 140, 846, 2246
Eco147I AGGCCT 1 cut(s) 2152
Eco24I GRGCYC 3 cut(s) 37, 831, 2027
Eco47I GGWCC 5 cut(s) 1472, 1722, 1962, 1976, 2204
Eco52I CGGCCG 1 cut(s) 1890
Eco53kI GAGCTC 2 cut(s) 829, 2025
Eco57I CTGAAG 1 cut(s) 2583
Eco88I CYCGRG 1 cut(s) 2229
EcoICRI GAGCTC 2 cut(s) 829, 2025
EcoO109I RGGNCCY 1 cut(s) 1962
EcoRI GAATTC 3 cut(s) 704, 2287, 2614
EcoRII CCWGG 1 cut(s) 2152
EcoT14I CCWWGG 3 cut(s) 140, 846, 2246
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 3 cut(s) 37, 831, 2027
ErhI CCWWGG 3 cut(s) 140, 846, 2246
Esp3I CGTCTC 1 cut(s) 12
FaqI GGGAC 4 cut(s) 1354, 1922, 1975, 2617
FauI CCCGC 1 cut(s) 1478
FbaI TGATCA 1 cut(s) 1002
Fnu4HI GCNGC 9 cut(s) 530, 753, 1637, 1661, 1890, 2119, 2122, 2437, 2595
FriOI GRGCYC 3 cut(s) 37, 831, 2027
Fsp4HI GCNGC 9 cut(s) 530, 753, 1637, 1661, 1890, 2119, 2122, 2437, 2595
FspBI CTAG 5 cut(s) 125, 1295, 1826, 2115, 2717
GlaI GCGC 2 cut(s) 498, 1320
GluI GCNGC 9 cut(s) 530, 753, 1637, 1661, 1890, 2119, 2122, 2437, 2595
GsaI CCCAGC 1 cut(s) 1581
GsuI CTGGAG 8 cut(s) 398, 594, 600, 1344, 2175, 2208, 2375, 2543
HaeIII GGCC 4 cut(s) 1892, 2109, 2152, 2308
HapII CCGG 2 cut(s) 919, 1725
HgaI GACGC 1 cut(s) 130
HhaI GCGC 2 cut(s) 499, 1321
Hin1I GRCGYC 2 cut(s) 5, 2351
Hin6I GCGC 2 cut(s) 497, 1319
HinP1I GCGC 2 cut(s) 497, 1319
HincII GTYRAC 2 cut(s) 1654, 2623
HindII GTYRAC 2 cut(s) 1654, 2623
HpaI GTTAAC 2 cut(s) 1654, 2623
HpaII CCGG 2 cut(s) 919, 1725
HphI GGTGA 4 cut(s) 92, 1798, 1859, 2397
Hpy166II GTNNAC 6 cut(s) 244, 689, 1310, 1654, 2575, 2623
Hpy8I GTNNAC 6 cut(s) 244, 689, 1310, 1654, 2575, 2623
HpyCH4III ACNGT 4 cut(s) 428, 1668, 2195, 2518
HpyCH4IV ACGT 2 cut(s) 5, 2351
HpyF10VI GCNNNNNNNGC 6 cut(s) 586, 1327, 1364, 1898, 2115, 2442
HpyF3I CTNAG 6 cut(s) 350, 623, 1368, 2180, 2264, 2376
HpySE526I ACGT 2 cut(s) 5, 2351
Hsp92I GRCGYC 2 cut(s) 5, 2351
HspAI GCGC 2 cut(s) 497, 1319
Kpn2I TCCGGA 1 cut(s) 1724
KpnI GGTACC 1 cut(s) 2221
Ksp22I TGATCA 1 cut(s) 1002
KspAI GTTAAC 2 cut(s) 1654, 2623
LmnI GCTCC 6 cut(s) 32, 613, 619, 848, 1363, 2725
Lsp1109I GCAGC 8 cut(s) 541, 764, 1623, 1647, 2130, 2133, 2448, 2606
LweI GCATC 6 cut(s) 487, 562, 781, 1446, 1472, 1636
MaeI CTAG 5 cut(s) 125, 1295, 1826, 2115, 2717
MaeII ACGT 2 cut(s) 5, 2351
MaeIII GTNAC 9 cut(s) 83, 428, 758, 866, 1290, 1786, 2403, 2512, 2648
MfeI CAATTG 2 cut(s) 44, 298
MflI RGATCY 4 cut(s) 205, 344, 1690, 1796
MhlI GDGCHC 4 cut(s) 37, 831, 845, 2027
MlyI GAGTC 3 cut(s) 7, 1298, 2534
MmeI TCCRAC 1 cut(s) 1621
Mph1103I ATGCAT 1 cut(s) 883
MroI TCCGGA 1 cut(s) 1724
MroXI GAANNNNTTC 3 cut(s) 1550, 1737, 2343
MspCI CTTAAG 1 cut(s) 1871
MspI CCGG 2 cut(s) 919, 1725
MspR9I CCNGG 2 cut(s) 920, 2154
MunI CAATTG 2 cut(s) 44, 298
Mva1269I GAATGC 2 cut(s) 2171, 2377
MvaI CCWGG 1 cut(s) 2154
MwoI GCNNNNNNNGC 6 cut(s) 586, 1327, 1364, 1898, 2115, 2442
NciI CCSGG 1 cut(s) 920
NcoI CCATGG 1 cut(s) 140
NheI GCTAGC 1 cut(s) 2716
NlaIV GGNNCC 6 cut(s) 34, 422, 1359, 1963, 2206, 2219
NmuCI GTSAC 5 cut(s) 428, 1290, 1786, 2403, 2512
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 2 cut(s) 885, 2729
PaeI GCATGC 1 cut(s) 885
PaeR7I CTCGAG 1 cut(s) 2229
PceI AGGCCT 1 cut(s) 2152
PciI ACATGT 1 cut(s) 2725
PctI GAATGC 2 cut(s) 2171, 2377
PdmI GAANNNNTTC 3 cut(s) 1550, 1737, 2343
PfeI GAWTC 7 cut(s) 181, 911, 1298, 1400, 1619, 1733, 2491
PkrI GCNGC 9 cut(s) 531, 754, 1638, 1662, 1891, 2120, 2123, 2438, 2596
PleI GAGTC 3 cut(s) 7, 1297, 2533
PpsI GAGTC 3 cut(s) 7, 1297, 2533
PpuMI RGGWCCY 1 cut(s) 1962
PscI ACATGT 1 cut(s) 2725
Psp124BI GAGCTC 2 cut(s) 831, 2027
Psp5II RGGWCCY 1 cut(s) 1962
Psp6I CCWGG 1 cut(s) 2152
PspFI CCCAGC 1 cut(s) 1577
PspGI CCWGG 1 cut(s) 2152
PspN4I GGNNCC 6 cut(s) 34, 422, 1359, 1963, 2206, 2219
PspPI GGNCC 6 cut(s) 1472, 1722, 1962, 1976, 2108, 2204
PspPPI RGGWCCY 1 cut(s) 1962
PstNI CAGNNNCTG 1 cut(s) 1367
PsuI RGATCY 4 cut(s) 205, 344, 1690, 1796
RsaI GTAC 8 cut(s) 543, 1053, 1189, 1230, 1311, 1629, 2094, 2219
RsaNI GTAC 8 cut(s) 542, 1052, 1188, 1229, 1310, 1628, 2093, 2218
SacI GAGCTC 2 cut(s) 831, 2027
SatI GCNGC 9 cut(s) 530, 753, 1637, 1661, 1890, 2119, 2122, 2437, 2595
Sau96I GGNCC 6 cut(s) 1472, 1722, 1962, 1976, 2108, 2204
SchI GAGTC 3 cut(s) 7, 1298, 2534
ScrFI CCNGG 2 cut(s) 920, 2154
SduI GDGCHC 4 cut(s) 37, 831, 845, 2027
SfaNI GCATC 6 cut(s) 487, 562, 781, 1446, 1472, 1636
SfcI CTRYAG 1 cut(s) 1902
Sfr274I CTCGAG 1 cut(s) 2229
SinI GGWCC 5 cut(s) 1472, 1722, 1962, 1976, 2204
SlaI CTCGAG 1 cut(s) 2229
SmiI ATTTAAAT 1 cut(s) 1146
SmlI CTYRAG 4 cut(s) 1631, 1871, 2020, 2229
SmoI CTYRAG 4 cut(s) 1631, 1871, 2020, 2229
SphI GCATGC 1 cut(s) 885
SseBI AGGCCT 1 cut(s) 2152
SsiI CCGC 2 cut(s) 1485, 1889
SspI AATATT 1 cut(s) 1549
SspMI CTAG 5 cut(s) 125, 1295, 1826, 2115, 2717
SstI GAGCTC 2 cut(s) 831, 2027
StuI AGGCCT 1 cut(s) 2152
StyD4I CCNGG 2 cut(s) 918, 2152
StyI CCWWGG 3 cut(s) 140, 846, 2246
SwaI ATTTAAAT 1 cut(s) 1146
TaaI ACNGT 4 cut(s) 428, 1668, 2195, 2518
TaiI ACGT 2 cut(s) 8, 2354
TaqI TCGA 3 cut(s) 720, 2230, 2425
TatI WGTACW 3 cut(s) 541, 1051, 1309
TauI GCSGC 1 cut(s) 1892
TfiI GAWTC 7 cut(s) 181, 911, 1298, 1400, 1619, 1733, 2491
TscAI CASTG 9 cut(s) 433, 741, 1080, 1503, 1629, 1848, 2446, 2521, 2564
TseFI GTSAC 5 cut(s) 428, 1290, 1786, 2403, 2512
TseI GCWGC 8 cut(s) 529, 752, 1636, 1660, 2118, 2121, 2436, 2594
Tsp45I GTSAC 5 cut(s) 428, 1290, 1786, 2403, 2512
TspGWI ACGGA 1 cut(s) 1968
TspRI CASTG 9 cut(s) 433, 741, 1080, 1503, 1629, 1848, 2446, 2521, 2564
Vha464I CTTAAG 1 cut(s) 1871
VpaK11BI GGWCC 5 cut(s) 1472, 1722, 1962, 1976, 2204
XbaI TCTAGA 1 cut(s) 1825
XceI RCATGY 2 cut(s) 885, 2729
XhoI CTCGAG 1 cut(s) 2229
XmnI GAANNNNTTC 3 cut(s) 1550, 1737, 2343
XspI CTAG 5 cut(s) 125, 1295, 1826, 2115, 2717
ZraI GACGTC 2 cut(s) 6, 2352
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.