RchiOBHm_Chr7g0186621

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000408
Physical Location & Seq
Forward (+)
6465235 .. 6469469
4235 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16657

Sequence Viewer

Length: 2694 bp
ATGAGGAATGTTCAGGAGAACTTACTATTGGGGAGGAACCACAGTGACAAACAAGATGATTTGGACAAAAAAATGTCTGAGTTATTGAAAGTGCATCAGGAAGTTCTGAAAGCGCAGAAAGAAATGTTTGATATGATGAGAGTGCAGCATGAGGCTGTACATGAGGCAATGCAGAGAATTAGAGCTGATGCTGTGCAAAATGTGCAAGGAGAAACTGCCCCTGCTCCAGCTCCAGCTCCAGCTCTAAGAATAGTATTTTGGGTGCTGTATGATGTTGTTATACAAGTGAAGGTCAAGAATATAATGTGTAAACGCCTCCTTCAAGAATTCAAATCCACGCTCGACTTCTTAAAACCACTGATAGAAGAAATGGCAGAAAGTAACAAACTATTGCATCTTCCAGAGCAGGAACAAGAGAAGTTTATTATTCAAATGAGGGAGGGTGTCGAGCTCATTGGCAAGTGCTCCAAGGTTAGCAAGTGGCATAGTTACAAAAAGTATGCATGCACCAACAAACTTCTTAAATTAGATGAATCTCTCCGGGGGCTATTCCATAAAATGAGTATGCAGATTGCAATCCATGTGAAGGAAGGTTTGATTTCGATAAGTAGTATGGAGGCAATGATCAAGAAAATTGAAGAGAGTGGTGTGATACAAAATCAAAGTCAAGTTGTACTCACTAAACCACAACCTCCACTGCATGAAGCTGGATTGGATGTTCTGGATACGCATGGAAGTAAGGATGTGGAGGGAACAGGAGATGATTTAAATGAGAAAGAGGTTGGCGTGAAGCAAATTGGAAGAGATGGTACGAGACAGAATGAAAAAGAGATTGAAGGTTTGAGTGAAGTACCTGAAAATTCATCCCCTAAAACTACATTGGATGTGCAAGGAACAAAGGATATAAATGAGTCACTAGATTCAGAAAGTGTACATATAGCGCAGACCAAGCAAAGTCAAGGGACAAGGATGGTTGAAGCTCCAGTTGCTGAGTCATATAAATTTCCATTCCTTATGGTTGAATCGGATGTGTATTTGAGGGAATTAAAGAAGAAGATTTTTGAGGATCAGGTGGCGATGCTTGTGATTACAGGTCCAGCAGGATGCGGGAAAACCACACTGGCAAGAATGTTTTGTGAAGATCAGGAGGTCAAAATGAAATTCAAGAATATTTTCTTTGTCCATGTTTCAGAAAATCCCAGATTGGATTTTATTGTCCAACAACTATATGAACAAAAGGATTCAGTGGTACTTGAGCTGCAAGAAGATGCAGTTAACTGGCTGCAACAGTTTCTAAAGGAAACAGGAGGAGACCCTTCGTTGTTGGTCTTGGATGATGTTTGGTCCGGATCAGAATCTCTTCTTGATAAGTTTGATGGATTAAGAACTTCAATGCACAAGATTTTGGTGACATCAAGATCTGAATTTCCACGATTTGATTGTTATCTAGAAAACCTAAAATCACTGGATGATGAAGAGGAAATGGCTCATCTTAAGGGAAAGGTCGTGGCGGCCGTGAGAGCTACAGGGGACTACCCAGAAAGAGTGGGCAAACCTATATGTGAGTATTATTTAAAGACTGGGACCTGTAAATTTGGTCCGTCCTGCAAGTTCCATCATCCGAAACAGGGAATTGGATACTTGAGCTCTTTGTATGGACTGACACAGCTATCTTCTCCCACCCATGCCCTTGAAAGACCTTATACCTCTGTCGTACCATCATCAGTTGGCCCTTCTAGCAGCAGCCCAAATGAACAAGCCTTCCCAGAGAGGCCTAGAGAACCTGAATGCCAGCATTACCTGAGAACTGGATACTATAAATTTGTACCCTCATGTCGGTACCACCATCCTCGAGATCAGATTGCACCAAGGACAAATTGTCTCCTCAGTCCAGTTGGTCTTCTTGGCAATTCAGGCTCTAATGCTTGGCCTTCATTCAGTCAAGTGTCATTGTCAATGGAAGATTTTCAGACGTCTTTGGATTTGAAATTGAATGCTCAGCATAGCCTTCTCCAGACACCAATGTCACCTTGTGTATCCATAAGTCGAAGAATAAGCAGCAATGACAAGTCCAGTCAGTCAGATGTAGAGGATAAGAGTGAGCTTGATGAGATTCAAAGCCAAATTTCAGGTGTCACTGTTCCGGAGTCTCTACATGATCCACTTGAAAGTAAAGATCAATGTCCAATGAATGTGAACAACACTTTGGAATATGCAGCTTCAGGGACAACTCCGAATTCAGTTAACGTTGATGGTCAGTCTGTTACTTGTAACAGCAGTGAGATTGGAAGCATATCACAGAAGCAGGGAGTTAAAGAAAAAGTTGCATTTAGAACAAAATCAGAGATCGAGATATTGGATGATGGATTCAAATGGAGGAAGTATGGGAAAAAATTTGGGAAGAACAGCACAAATCCAAGGAATTACTATAAGTGTGCATATGAAGGCTGCCCAGTGAAAAAGAGAGTTGAAAGAGATGGACAAGATCCAAGTTATGTAATTACGTGTTATAACGGGGTTCATAACCATACAAGCCTTGGCAAAAGAACCCAGCGAGTACCCGCCAGCCACCCTCAACCTCTCCCTTTTCCCCTCCCTATAGGGAAGGAAGAGAGCTTTACGTTCGATCATCTTGCAGACGATAATCAGGAAGAGAGCGTTCTGTTCGATCATCTTGCAGACCATATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

897

Amino Acids

101.06

Weight (kDa)

5.88

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 80 - 210 5.5e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 346 - 475 2.3e-14 NB-ARC domain
AAA_22 PF13401 359 - 447 3.4e-06 AAA domain
zf-CCCH PF00642 518 - 542 9e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
WRKY PF03106 787 - 843 1.2e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2511
AasI GACNNNNNNGTC 1 cut(s) 2023
AatII GACGTC 1 cut(s) 1976
Acc65I GGTACC 1 cut(s) 1839
AccB1I GGYRCC 1 cut(s) 1839
AccIII TCCGGA 2 cut(s) 1346, 2143
AciI CCGC 3 cut(s) 1107, 1511, 2562
AclI AACGTT 1 cut(s) 2247
AclWI GGATC 4 cut(s) 1074, 1357, 2153, 2480
AcoI YGGCCR 1 cut(s) 1512
AcuI CTGAAG 1 cut(s) 2205
AcyI GRCGYC 1 cut(s) 1973
AdeI CACNNNGTG 1 cut(s) 2033
AfiI CCNNNNNNNGG 3 cut(s) 586, 1628, 1836
AflII CTTAAG 1 cut(s) 1493
AflIII ACRYGT 1 cut(s) 2504
AhdI GACNNNNNGTC 1 cut(s) 1878
AjuI GAANNNNNNNTTGG 6 cut(s) 11, 43, 241, 273, 2189, 2221
AloI GAACNNNNNNTCC 4 cut(s) 702, 734, 1746, 1778
Alw21I GWGCWC 3 cut(s) 453, 467, 1649
Alw26I GTCTC 4 cut(s) 808, 1305, 1886, 2154
AlwI GGATC 4 cut(s) 1074, 1357, 2153, 2480
AlwNI CAGNNNCTG 1 cut(s) 989
Ama87I CYCGRG 1 cut(s) 1851
Aor13HI TCCGGA 2 cut(s) 1346, 2143
AoxI GGCC 4 cut(s) 1512, 1729, 1772, 1928
ApeKI GCWGC 8 cut(s) 145, 1258, 1282, 1740, 1743, 2058, 2216, 2448
ArsI GACNNNNNNTTYG 2 cut(s) 649, 681
Asp700I GAANNNNTTC 3 cut(s) 1172, 1359, 1965
Asp718I GGTACC 1 cut(s) 1839
AspLEI GCGC 2 cut(s) 115, 943
AspS9I GGNCC 5 cut(s) 1094, 1344, 1584, 1598, 1730
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 2 cut(s) 1420, 2019
AvaI CYCGRG 1 cut(s) 1851
AvaII GGWCC 4 cut(s) 1094, 1344, 1584, 1598
BanI GGYRCC 1 cut(s) 1839
BanII GRGCYC 2 cut(s) 453, 1649
BarI GAAGNNNNNNTAC 2 cut(s) 793, 825
BbsI GAAGAC 1 cut(s) 1892
Bbv12I GWGCWC 3 cut(s) 453, 467, 1649
BbvI GCAGC 8 cut(s) 157, 1245, 1269, 1752, 1755, 2070, 2228, 2435
BccI CCATC 9 cut(s) 800, 964, 1370, 1623, 1726, 1854, 2246, 2357, 2471
BceAI ACGGC 1 cut(s) 1499
BcgI CGANNNNNNTGC 4 cut(s) 2615, 2649, 2657, 2691
BciVI GTATCC 4 cut(s) 718, 1631, 1805, 2047
BclI TGATCA 1 cut(s) 624
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 4 cut(s) 808, 1305, 1886, 2154
BfaI CTAG 4 cut(s) 917, 1448, 1737, 1776
BfmI CTRYAG 2 cut(s) 1524, 2598
BfrI CTTAAG 1 cut(s) 1493
BfuI GTATCC 4 cut(s) 718, 1631, 1805, 2047
BglII AGATCT 1 cut(s) 1418
BisI GCNGC 9 cut(s) 146, 1259, 1283, 1512, 1741, 1744, 2059, 2217, 2449
BlpI GCTNAGC 1 cut(s) 1998
BlsI GCNGC 9 cut(s) 147, 1260, 1284, 1513, 1742, 1745, 2060, 2218, 2450
Bme1390I CCNGG 1 cut(s) 542
Bme18I GGWCC 4 cut(s) 1094, 1344, 1584, 1598
BmeRI GACNNNNNGTC 1 cut(s) 1878
BmeT110I CYCGRG 1 cut(s) 1851
BmgT120I GGNCC 5 cut(s) 1094, 1344, 1584, 1598, 1730
BmiI GGNNCC 3 cut(s) 38, 1585, 1841
BmrFI CCNGG 1 cut(s) 542
BmrI ACTGGG 2 cut(s) 1590, 2447
BmsI GCATC 6 cut(s) 103, 178, 403, 1068, 1094, 1258
BmuI ACTGGG 2 cut(s) 1590, 2447
BpiI GAAGAC 1 cut(s) 1892
BpmI CTGGAG 5 cut(s) 210, 216, 222, 966, 1997
Bpu1102I GCTNAGC 1 cut(s) 1998
BpuEI CTTGAG 2 cut(s) 1274, 1663
BpuMI CCSGG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 2505
BsaBI GATNNNNATC 3 cut(s) 575, 1354, 1443
BsaHI GRCGYC 1 cut(s) 1973
BsaI GGTCTC 1 cut(s) 1305
BsaJI CCNNGG 5 cut(s) 468, 541, 1868, 2417, 2536
BsaWI WCCGGW 2 cut(s) 1346, 2143
Bsc4I CCNNNNNNNGG 3 cut(s) 586, 1628, 1836
Bse1I ACTGG 9 cut(s) 983, 1125, 1283, 1470, 1585, 1813, 1892, 2073, 2453
Bse3DI GCAATG 3 cut(s) 174, 627, 2068
Bse8I GATNNNNATC 3 cut(s) 575, 1354, 1443
BseAI TCCGGA 2 cut(s) 1346, 2143
BseDI CCNNGG 5 cut(s) 468, 541, 1868, 2417, 2536
BseJI GATNNNNATC 3 cut(s) 575, 1354, 1443
BseLI CCNNNNNNNGG 3 cut(s) 586, 1628, 1836
BseMI GCAATG 3 cut(s) 174, 627, 2068
BseMII CTCAG 5 cut(s) 69, 981, 1793, 1900, 2012
BseNI ACTGG 9 cut(s) 983, 1125, 1283, 1470, 1585, 1813, 1892, 2073, 2453
BseRI GAGGAG 2 cut(s) 1323, 1874
BseX3I CGGCCG 1 cut(s) 1512
BseXI GCAGC 8 cut(s) 157, 1245, 1269, 1752, 1755, 2070, 2228, 2435
BseYI CCCAGC 1 cut(s) 2550
BsgI GTGCAG 1 cut(s) 164
Bsh1285I CGRYCG 1 cut(s) 1515
BshFI GGCC 4 cut(s) 1514, 1731, 1774, 1930
BshNI GGYRCC 1 cut(s) 1839
BsiEI CGRYCG 1 cut(s) 1515
BsiHKAI GWGCWC 3 cut(s) 453, 467, 1649
BsiHKCI CYCGRG 1 cut(s) 1851
BsiSI CCGG 3 cut(s) 541, 1347, 2144
BslFI GGGAC 4 cut(s) 976, 1544, 1597, 2239
BslI CCNNNNNNNGG 3 cut(s) 586, 1628, 1836
BsmAI GTCTC 4 cut(s) 808, 1305, 1886, 2154
BsmFI GGGAC 4 cut(s) 976, 1544, 1597, 2239
BsmI GAATGC 2 cut(s) 1793, 1999
BsnI GGCC 4 cut(s) 1514, 1731, 1774, 1930
Bso31I GGTCTC 1 cut(s) 1305
BsoBI CYCGRG 1 cut(s) 1851
Bsp1286I GDGCHC 3 cut(s) 453, 467, 1649
Bsp13I TCCGGA 2 cut(s) 1346, 2143
Bsp1407I TGTACA 2 cut(s) 157, 931
Bsp1720I GCTNAGC 1 cut(s) 1998
BspACI CCGC 3 cut(s) 1107, 1511, 2562
BspANI GGCC 4 cut(s) 1514, 1731, 1774, 1930
BspCNI CTCAG 5 cut(s) 70, 982, 1794, 1899, 2011
BspEI TCCGGA 2 cut(s) 1346, 2143
BspLI GGNNCC 3 cut(s) 38, 1585, 1841
BspPI GGATC 4 cut(s) 1074, 1357, 2153, 2480
BspT107I GGYRCC 1 cut(s) 1839
BspTI CTTAAG 1 cut(s) 1493
BspTNI GGTCTC 1 cut(s) 1305
BsrDI GCAATG 3 cut(s) 174, 627, 2068
BsrGI TGTACA 2 cut(s) 157, 931
BsrI ACTGG 9 cut(s) 983, 1125, 1283, 1470, 1585, 1813, 1892, 2073, 2453
BssECI CCNNGG 5 cut(s) 468, 541, 1868, 2417, 2536
BssNI GRCGYC 1 cut(s) 1973
BssT1I CCWWGG 4 cut(s) 468, 1868, 2417, 2536
Bst4CI ACNGT 3 cut(s) 44, 1290, 2140
Bst6I CTCTTC 6 cut(s) 633, 796, 1365, 1470, 2604, 2646
BstACI GRCGYC 1 cut(s) 1973
BstAFI CTTAAG 1 cut(s) 1493
BstAPI GCANNNNNTGC 1 cut(s) 202
BstAUI TGTACA 2 cut(s) 157, 931
BstBAI YACGTR 1 cut(s) 2505
BstC8I GCNNGC 3 cut(s) 505, 1793, 2566
BstDEI CTNAG 6 cut(s) 78, 245, 990, 1802, 1886, 1998
BstHHI GCGC 2 cut(s) 115, 943
BstMAI GTCTC 4 cut(s) 808, 1305, 1886, 2154
BstMCI CGRYCG 1 cut(s) 1515
BstMWI GCNNNNNNNGC 6 cut(s) 202, 949, 986, 1520, 1737, 1914
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 2 cut(s) 1524, 2598
BstV1I GCAGC 8 cut(s) 157, 1245, 1269, 1752, 1755, 2070, 2228, 2435
BstV2I GAAGAC 1 cut(s) 1892
BstX2I RGATCY 2 cut(s) 1418, 2485
BstYI RGATCY 2 cut(s) 1418, 2485
BstZI CGGCCG 1 cut(s) 1512
BsuI GTATCC 4 cut(s) 718, 1631, 1805, 2047
BsuRI GGCC 4 cut(s) 1514, 1731, 1774, 1930
BtgZI GCGATG 1 cut(s) 1091
BtsI GCAGTG 2 cut(s) 695, 2284
BtsIMutI CAGTG 9 cut(s) 49, 356, 695, 1118, 1251, 1463, 2136, 2284, 2460
Cac8I GCNNGC 3 cut(s) 505, 1793, 2566
CaiI CAGNNNCTG 1 cut(s) 989
CfoI GCGC 2 cut(s) 115, 943
Cfr13I GGNCC 5 cut(s) 1094, 1344, 1584, 1598, 1730
DdeI CTNAG 6 cut(s) 78, 245, 990, 1802, 1886, 1998
DraI TTTAAA 2 cut(s) 768, 1575
DraIII CACNNNGTG 1 cut(s) 2033
DrdI GACNNNNNNGTC 1 cut(s) 2023
DriI GACNNNNNGTC 1 cut(s) 1878
DseDI GACNNNNNNGTC 1 cut(s) 2023
EaeI YGGCCR 1 cut(s) 1512
EagI CGGCCG 1 cut(s) 1512
Eam1104I CTCTTC 6 cut(s) 633, 796, 1365, 1470, 2604, 2646
Eam1105I GACNNNNNGTC 1 cut(s) 1878
EarI CTCTTC 6 cut(s) 633, 796, 1365, 1470, 2604, 2646
Ecl136II GAGCTC 2 cut(s) 451, 1647
EclXI CGGCCG 1 cut(s) 1512
Eco130I CCWWGG 4 cut(s) 468, 1868, 2417, 2536
Eco147I AGGCCT 1 cut(s) 1774
Eco24I GRGCYC 2 cut(s) 453, 1649
Eco31I GGTCTC 1 cut(s) 1305
Eco47I GGWCC 4 cut(s) 1094, 1344, 1584, 1598
Eco52I CGGCCG 1 cut(s) 1512
Eco53kI GAGCTC 2 cut(s) 451, 1647
Eco57I CTGAAG 1 cut(s) 2205
Eco88I CYCGRG 1 cut(s) 1851
EcoICRI GAGCTC 2 cut(s) 451, 1647
EcoO109I RGGNCCY 1 cut(s) 1584
EcoRI GAATTC 2 cut(s) 326, 2236
EcoT14I CCWWGG 4 cut(s) 468, 1868, 2417, 2536
EcoT22I ATGCAT 1 cut(s) 505
EcoT38I GRGCYC 2 cut(s) 453, 1649
ErhI CCWWGG 4 cut(s) 468, 1868, 2417, 2536
FaqI GGGAC 4 cut(s) 976, 1544, 1597, 2239
FauI CCCGC 2 cut(s) 1100, 2569
FauNDI CATATG 1 cut(s) 2440
FbaI TGATCA 1 cut(s) 624
Fnu4HI GCNGC 9 cut(s) 146, 1259, 1283, 1512, 1741, 1744, 2059, 2217, 2449
FriOI GRGCYC 2 cut(s) 453, 1649
Fsp4HI GCNGC 9 cut(s) 146, 1259, 1283, 1512, 1741, 1744, 2059, 2217, 2449
FspBI CTAG 4 cut(s) 917, 1448, 1737, 1776
GlaI GCGC 2 cut(s) 114, 942
GluI GCNGC 9 cut(s) 146, 1259, 1283, 1512, 1741, 1744, 2059, 2217, 2449
GsaI CCCAGC 1 cut(s) 2554
GsuI CTGGAG 5 cut(s) 210, 216, 222, 966, 1997
HaeIII GGCC 4 cut(s) 1514, 1731, 1774, 1930
HapII CCGG 3 cut(s) 541, 1347, 2144
HhaI GCGC 2 cut(s) 115, 943
Hin1I GRCGYC 1 cut(s) 1973
Hin6I GCGC 2 cut(s) 113, 941
HinP1I GCGC 2 cut(s) 113, 941
HincII GTYRAC 2 cut(s) 1276, 2245
HindII GTYRAC 2 cut(s) 1276, 2245
HpaI GTTAAC 2 cut(s) 1276, 2245
HpaII CCGG 3 cut(s) 541, 1347, 2144
HphI GGTGA 2 cut(s) 1420, 2019
Hpy166II GTNNAC 5 cut(s) 311, 932, 1276, 2197, 2245
Hpy8I GTNNAC 5 cut(s) 311, 932, 1276, 2197, 2245
HpyCH4III ACNGT 3 cut(s) 44, 1290, 2140
HpyCH4IV ACGT 4 cut(s) 1973, 2247, 2504, 2621
HpyF10VI GCNNNNNNNGC 6 cut(s) 202, 949, 986, 1520, 1737, 1914
HpyF3I CTNAG 6 cut(s) 78, 245, 990, 1802, 1886, 1998
HpySE526I ACGT 4 cut(s) 1973, 2247, 2504, 2621
Hsp92I GRCGYC 1 cut(s) 1973
HspAI GCGC 2 cut(s) 113, 941
Kpn2I TCCGGA 2 cut(s) 1346, 2143
KpnI GGTACC 1 cut(s) 1843
Ksp22I TGATCA 1 cut(s) 624
KspAI GTTAAC 2 cut(s) 1276, 2245
LmnI GCTCC 5 cut(s) 229, 235, 241, 470, 985
Lsp1109I GCAGC 8 cut(s) 157, 1245, 1269, 1752, 1755, 2070, 2228, 2435
LweI GCATC 6 cut(s) 103, 178, 403, 1068, 1094, 1258
MaeI CTAG 4 cut(s) 917, 1448, 1737, 1776
MaeII ACGT 4 cut(s) 1973, 2247, 2504, 2621
MaeIII GTNAC 9 cut(s) 44, 380, 488, 912, 1408, 2025, 2134, 2263, 2270
MflI RGATCY 2 cut(s) 1418, 2485
MhlI GDGCHC 3 cut(s) 453, 467, 1649
MlyI GAGTC 3 cut(s) 920, 1001, 2156
MmeI TCCRAC 1 cut(s) 1243
Mph1103I ATGCAT 1 cut(s) 505
MroI TCCGGA 2 cut(s) 1346, 2143
MroXI GAANNNNTTC 3 cut(s) 1172, 1359, 1965
MspCI CTTAAG 1 cut(s) 1493
MspI CCGG 3 cut(s) 541, 1347, 2144
MspR9I CCNGG 1 cut(s) 542
Mva1269I GAATGC 2 cut(s) 1793, 1999
MwoI GCNNNNNNNGC 6 cut(s) 202, 949, 986, 1520, 1737, 1914
NciI CCSGG 1 cut(s) 542
NdeI CATATG 1 cut(s) 2440
NlaIV GGNNCC 3 cut(s) 38, 1585, 1841
NmuCI GTSAC 5 cut(s) 44, 912, 1408, 2025, 2134
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 1 cut(s) 507
PaeI GCATGC 1 cut(s) 507
PaeR7I CTCGAG 1 cut(s) 1851
PceI AGGCCT 1 cut(s) 1774
PcsI WCGNNNNNNNCGW 1 cut(s) 1512
PctI GAATGC 2 cut(s) 1793, 1999
PdmI GAANNNNTTC 3 cut(s) 1172, 1359, 1965
PfeI GAWTC 7 cut(s) 533, 920, 1022, 1241, 1355, 2113, 2367
PkrI GCNGC 9 cut(s) 147, 1260, 1284, 1513, 1742, 1745, 2060, 2218, 2450
PleI GAGTC 3 cut(s) 919, 1000, 2155
PpsI GAGTC 3 cut(s) 919, 1000, 2155
Ppu21I YACGTR 1 cut(s) 2505
PpuMI RGGWCCY 1 cut(s) 1584
PsiI TTATAA 1 cut(s) 2511
Psp124BI GAGCTC 2 cut(s) 453, 1649
Psp1406I AACGTT 1 cut(s) 2247
Psp5II RGGWCCY 1 cut(s) 1584
PspFI CCCAGC 1 cut(s) 2550
PspN4I GGNNCC 3 cut(s) 38, 1585, 1841
PspPI GGNCC 5 cut(s) 1094, 1344, 1584, 1598, 1730
PspPPI RGGWCCY 1 cut(s) 1584
PstNI CAGNNNCTG 1 cut(s) 989
PsuI RGATCY 2 cut(s) 1418, 2485
SacI GAGCTC 2 cut(s) 453, 1649
SatI GCNGC 9 cut(s) 146, 1259, 1283, 1512, 1741, 1744, 2059, 2217, 2449
Sau96I GGNCC 5 cut(s) 1094, 1344, 1584, 1598, 1730
SchI GAGTC 3 cut(s) 920, 1001, 2156
ScrFI CCNGG 1 cut(s) 542
SduI GDGCHC 3 cut(s) 453, 467, 1649
SfaNI GCATC 6 cut(s) 103, 178, 403, 1068, 1094, 1258
SfcI CTRYAG 2 cut(s) 1524, 2598
Sfr274I CTCGAG 1 cut(s) 1851
SinI GGWCC 4 cut(s) 1094, 1344, 1584, 1598
SlaI CTCGAG 1 cut(s) 1851
SmiI ATTTAAAT 1 cut(s) 768
SmlI CTYRAG 4 cut(s) 1253, 1493, 1642, 1851
SmoI CTYRAG 4 cut(s) 1253, 1493, 1642, 1851
SphI GCATGC 1 cut(s) 507
SseBI AGGCCT 1 cut(s) 1774
SsiI CCGC 3 cut(s) 1107, 1511, 2562
SspI AATATT 1 cut(s) 1171
SspMI CTAG 4 cut(s) 917, 1448, 1737, 1776
SstI GAGCTC 2 cut(s) 453, 1649
StuI AGGCCT 1 cut(s) 1774
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 4 cut(s) 468, 1868, 2417, 2536
SwaI ATTTAAAT 1 cut(s) 768
TaaI ACNGT 3 cut(s) 44, 1290, 2140
TaiI ACGT 4 cut(s) 1976, 2250, 2507, 2624
TaqI TCGA 8 cut(s) 342, 447, 602, 1852, 2047, 2349, 2625, 2667
TatI WGTACW 3 cut(s) 157, 673, 931
TauI GCSGC 1 cut(s) 1514
TfiI GAWTC 7 cut(s) 533, 920, 1022, 1241, 1355, 2113, 2367
TscAI CASTG 9 cut(s) 49, 363, 702, 1125, 1251, 1470, 2143, 2284, 2460
TseFI GTSAC 5 cut(s) 44, 912, 1408, 2025, 2134
TseI GCWGC 8 cut(s) 145, 1258, 1282, 1740, 1743, 2058, 2216, 2448
Tsp45I GTSAC 5 cut(s) 44, 912, 1408, 2025, 2134
TspGWI ACGGA 1 cut(s) 1590
TspRI CASTG 9 cut(s) 49, 363, 702, 1125, 1251, 1470, 2143, 2284, 2460
Vha464I CTTAAG 1 cut(s) 1493
VpaK11BI GGWCC 4 cut(s) 1094, 1344, 1584, 1598
XbaI TCTAGA 1 cut(s) 1447
XceI RCATGY 1 cut(s) 507
XcmI CCANNNNNNNNNTGG 1 cut(s) 2534
XhoI CTCGAG 1 cut(s) 1851
XmnI GAANNNNTTC 3 cut(s) 1172, 1359, 1965
XspI CTAG 4 cut(s) 917, 1448, 1737, 1776
ZraI GACGTC 1 cut(s) 1974
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.