Rw7G007150
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000408
Physical Location & Seq
Forward (+)
6181876 .. 6185716
3841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G007150.1

Sequence Viewer

Length: 2211 bp
ATGTTTGATATGATGAGAGTGCAGCATGAGGCTGTACATGAGGCAATGCAGAGAATTAGAGCTGATGCTGTGCAAAATGTGCAAGGAGAAACTGCCCCTGCTCCAGCTCCAGCTCTAAGAATAGTATTTTGGGTGCTGTATGACGTTGTTATACAAGTGAAGGTCAAGAATATAATGTGTAAACGCCTCCTTCAAGAATTCAAATCCACGCTCGACTTCTTAAAACCACTGATAGAAGAAATGGCAGAAAGTAACAAACTATTGCATCTTCCAGAGCAGGAACAAGAGAAGTTTATTATTCAAATGAGGGAGGGTGTCGAGCTCATTGACAAGTGCTCCAAGGTTAGCAAGTGGCATAGTTACAAAAAGTATGCATGCACCAACAAACTTCTTAAATTAGATGAATCTCTCCGGGGGCTATTCCATAAAATGAGTATGCAGATTGCAATCCATGTGAAGGAAGGTTTGATTTCGATAAGTAGTATGGAGGCAATGATCAAGAAAATTGAAGAGAGTGGTGTGATACAAAATCAAAGTCAAGTTGTACTCACTAAACCACAACCTCCACTGCATGAAGCTGGATTGGATGTTCTGGATACGCATGGAAGTAAGGATGAGGAGGGAACAGGAGATGATTTAAATGAGAAAGAGGTTGGCGTGAAGCAAATTGGAAGAGATGGTACGAGACAGAATGAAAAAGAGATTGAAGGTTTGAGTGAAGTACCTGAAAATTCATCCCCTAAAACTACATTGGATGTGCAAGGAACAAAGGATATAAATGAGTCACTAGATTCAGAAAGTGTACATATAGCGCAGACCAAGCAAAGTCAAGGGACAAGGATGGTTGAAGCTCCAGTTGCTGAGTCATATAAATTTCCATTCCTTATGGTTGAATCGGATGTGTATTTGAGGGAATTAAAGAAGAAGATTTTTGAGGATCAGGTGGCGATGCTTGTGATTACAGGTCCAGCAGGATGCGGGAAAACAACACTGGCAAGAATGTTTTGTGAAGATCAGGAGGTCGTGGCGGCCGTGAGAGCTACAGGGGACTACCCAGAAAGAGTGGGCAAACCTATATGTGAGTATTATTTAAAGACTGGGACCTGTAAATTTGGTCCGTCCTGCAAGTTCCATCATCCGAAACAGGGAATTGGATACTTGAGCTCTTTGTATGGACTGACACAGCTATCTTCTCCCACCCATGCCCTTGAAAGACCTTATACCTCTGTCGTACCATCATCAGTTGGCCCTTCTAGCAGCAGCCCAAATGAACAAGCCTTCCCAGAGAGGCCTAGAGAACCTGAATGCCAGCATTACCTGAGAACTGGATACTATAAATTTGTACCCTCATGTCGGTACCACCATCCTCGAGATCGGATTGCACCAAGGACAAATTGTCTCCTCAGTCCAGTTGGTCTTCTTGGCAATTCAGGCTCTAATGCTTGGCCTTCATTCAGTCAAGTGTCATTGTCAATGGAAGATTTTCAGACGTCTTTGGATTTGAAATTGAATGCTCAGCATAGCCTTCTCCAGACACCAATGTCACCTTGTGTATCCATAAGTCGAAGAATAAGCAGCAATGACAAGTCCAGTCAGTCAGATGTAGAGGATAAGAGTGAGCTTGATGAGATTCAAAGCCAAATTTCAGGTGTCACTGTTCCGGAGTCGCTACATGATCCACTTGAAAGTAAAGATCAATGTCCAATGAATGTGAACAACACTTTGGAATATGCAGCTTCAGGGACAACTCCGAATTCAGTTAACGTTGATGGTCAGTCTGTTGCTTGTAACAGCAGTGAGATTGGAAGCATATCACAGAAGCAGGGAGTTAAAGAAAGAGTTGCATTTCGAACAAAATCAGAGATGGAGATATTGGATGATGGATTCAAATGGAGGAAGTATGGGAAAAAAAGTAGGAAGAACAGCACATATCCAAGGAATTACTATAAGTGTGCATATGAAGGCTGCCCAGTGAAAAAGAGAGTTGAAAGAGATAGACAAGATCCTAGTTATCTAATTACATGTTATGATGGGGTTCATGAACATACAAGCCTTGGCAAAAGTACCCAGCGAGTACTCGCCAGCCACCCTCAACCTCTCCCTTTTGCCCTCCCTATAGGGAAGGAAGAAAGCTTTCTGTTCGATCATCTTGCAGACGATAATCAGGAAGAGAGCGTTCTGTTCGATCATCTTGCAGACCGTATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

736

Amino Acids

82.6

Weight (kDa)

6.04

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 37 - 167 3.5e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
zf-CCCH PF00642 357 - 381 7.2e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
WRKY PF03106 626 - 682 2.5e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1540
AatII GACGTC 1 cut(s) 1493
Acc65I GGTACC 1 cut(s) 1356
AccB1I GGYRCC 1 cut(s) 1356
AccIII TCCGGA 1 cut(s) 1660
AciI CCGC 2 cut(s) 978, 1028
AclI AACGTT 1 cut(s) 1764
AclWI GGATC 3 cut(s) 945, 1670, 1997
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 7 cut(s) 197, 730, 872, 1109, 1337, 1641, 1753
AcuI CTGAAG 1 cut(s) 1722
AcyI GRCGYC 1 cut(s) 1490
AdeI CACNNNGTG 1 cut(s) 1550
AfiI CCNNNNNNNGG 3 cut(s) 457, 1145, 1353
AflIII ACRYGT 1 cut(s) 2021
AhdI GACNNNNNGTC 1 cut(s) 1395
AjuI GAANNNNNNNTTGG 4 cut(s) 112, 144, 1706, 1738
AloI GAACNNNNNNTCC 4 cut(s) 573, 605, 1263, 1295
Alw21I GWGCWC 3 cut(s) 324, 338, 1166
Alw26I GTCTC 2 cut(s) 679, 1403
AlwI GGATC 3 cut(s) 945, 1670, 1997
AlwNI CAGNNNCTG 1 cut(s) 860
Ama87I CYCGRG 1 cut(s) 1368
Aor13HI TCCGGA 1 cut(s) 1660
AoxI GGCC 4 cut(s) 1029, 1246, 1289, 1445
ApeKI GCWGC 6 cut(s) 22, 1257, 1260, 1575, 1733, 1965
ApoI RAATTY 7 cut(s) 197, 730, 872, 1109, 1337, 1641, 1753
ArsI GACNNNNNNTTYG 2 cut(s) 520, 552
Asp700I GAANNNNTTC 2 cut(s) 1482, 2133
Asp718I GGTACC 1 cut(s) 1356
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 4 cut(s) 965, 1101, 1115, 1247
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 1536
AsuII TTCGAA 1 cut(s) 1849
AvaI CYCGRG 1 cut(s) 1368
AvaII GGWCC 3 cut(s) 965, 1101, 1115
BanI GGYRCC 1 cut(s) 1356
BanII GRGCYC 2 cut(s) 324, 1166
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BbsI GAAGAC 1 cut(s) 1409
Bbv12I GWGCWC 3 cut(s) 324, 338, 1166
BbvI GCAGC 6 cut(s) 34, 1269, 1272, 1587, 1745, 1952
BccI CCATC 9 cut(s) 671, 835, 1140, 1243, 1371, 1763, 1858, 1874, 2024
BceAI ACGGC 1 cut(s) 1016
BcgI CGANNNNNNTGC 4 cut(s) 2132, 2166, 2174, 2208
BciVI GTATCC 4 cut(s) 589, 1148, 1322, 1564
BclI TGATCA 1 cut(s) 495
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 679, 1403
BfaI CTAG 4 cut(s) 788, 1254, 1293, 2007
BfmI CTRYAG 2 cut(s) 1041, 2115
BfuI GTATCC 4 cut(s) 589, 1148, 1322, 1564
BisI GCNGC 7 cut(s) 23, 1029, 1258, 1261, 1576, 1734, 1966
BlpI GCTNAGC 1 cut(s) 1515
BlsI GCNGC 7 cut(s) 24, 1030, 1259, 1262, 1577, 1735, 1967
BmcAI AGTACT 1 cut(s) 2076
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 3 cut(s) 965, 1101, 1115
BmeRI GACNNNNNGTC 1 cut(s) 1395
BmeT110I CYCGRG 1 cut(s) 1368
BmgT120I GGNCC 4 cut(s) 965, 1101, 1115, 1247
BmiI GGNNCC 2 cut(s) 1102, 1358
BmrFI CCNGG 1 cut(s) 413
BmrI ACTGGG 2 cut(s) 1107, 1964
BmsI GCATC 4 cut(s) 55, 274, 939, 965
BmuI ACTGGG 2 cut(s) 1107, 1964
BpiI GAAGAC 1 cut(s) 1409
BpmI CTGGAG 4 cut(s) 87, 93, 837, 1514
Bpu1102I GCTNAGC 1 cut(s) 1515
Bpu14I TTCGAA 1 cut(s) 1849
BpuEI CTTGAG 1 cut(s) 1180
BpuMI CCSGG 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 446
BsaHI GRCGYC 1 cut(s) 1490
BsaJI CCNNGG 5 cut(s) 339, 412, 1385, 1934, 2053
BsaWI WCCGGW 1 cut(s) 1660
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 3 cut(s) 457, 1145, 1353
Bse1I ACTGG 7 cut(s) 854, 996, 1102, 1330, 1409, 1590, 1970
Bse3DI GCAATG 3 cut(s) 51, 498, 1585
Bse8I GATNNNNATC 1 cut(s) 446
BseAI TCCGGA 1 cut(s) 1660
BseDI CCNNGG 5 cut(s) 339, 412, 1385, 1934, 2053
BseJI GATNNNNATC 1 cut(s) 446
BseLI CCNNNNNNNGG 3 cut(s) 457, 1145, 1353
BseMI GCAATG 3 cut(s) 51, 498, 1585
BseMII CTCAG 4 cut(s) 852, 1310, 1417, 1529
BseNI ACTGG 7 cut(s) 854, 996, 1102, 1330, 1409, 1590, 1970
BseRI GAGGAG 2 cut(s) 632, 1391
BseX3I CGGCCG 1 cut(s) 1029
BseXI GCAGC 6 cut(s) 34, 1269, 1272, 1587, 1745, 1952
BseYI CCCAGC 1 cut(s) 2067
BsgI GTGCAG 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 1032
BshFI GGCC 4 cut(s) 1031, 1248, 1291, 1447
BshNI GGYRCC 1 cut(s) 1356
BsiEI CGRYCG 1 cut(s) 1032
BsiHKAI GWGCWC 3 cut(s) 324, 338, 1166
BsiHKCI CYCGRG 1 cut(s) 1368
BsiSI CCGG 2 cut(s) 412, 1661
BslFI GGGAC 4 cut(s) 847, 1061, 1114, 1756
BslI CCNNNNNNNGG 3 cut(s) 457, 1145, 1353
BsmAI GTCTC 2 cut(s) 679, 1403
BsmFI GGGAC 4 cut(s) 847, 1061, 1114, 1756
BsmI GAATGC 2 cut(s) 1310, 1516
BsnI GGCC 4 cut(s) 1031, 1248, 1291, 1447
BsoBI CYCGRG 1 cut(s) 1368
Bsp119I TTCGAA 1 cut(s) 1849
Bsp1286I GDGCHC 3 cut(s) 324, 338, 1166
Bsp13I TCCGGA 1 cut(s) 1660
Bsp1407I TGTACA 2 cut(s) 34, 802
Bsp143I GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
Bsp1720I GCTNAGC 1 cut(s) 1515
BspACI CCGC 2 cut(s) 978, 1028
BspANI GGCC 4 cut(s) 1031, 1248, 1291, 1447
BspCNI CTCAG 4 cut(s) 853, 1311, 1416, 1528
BspEI TCCGGA 1 cut(s) 1660
BspHI TCATGA 1 cut(s) 2038
BspLI GGNNCC 2 cut(s) 1102, 1358
BspPI GGATC 3 cut(s) 945, 1670, 1997
BspT104I TTCGAA 1 cut(s) 1849
BspT107I GGYRCC 1 cut(s) 1356
BsrDI GCAATG 3 cut(s) 51, 498, 1585
BsrGI TGTACA 2 cut(s) 34, 802
BsrI ACTGG 7 cut(s) 854, 996, 1102, 1330, 1409, 1590, 1970
BssECI CCNNGG 5 cut(s) 339, 412, 1385, 1934, 2053
BssMI GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
BssNI GRCGYC 1 cut(s) 1490
BssT1I CCWWGG 4 cut(s) 339, 1385, 1934, 2053
Bst4CI ACNGT 2 cut(s) 1657, 2201
Bst6I CTCTTC 3 cut(s) 504, 667, 2163
BstACI GRCGYC 1 cut(s) 1490
BstAPI GCANNNNNTGC 1 cut(s) 79
BstAUI TGTACA 2 cut(s) 34, 802
BstBI TTCGAA 1 cut(s) 1849
BstC8I GCNNGC 3 cut(s) 376, 1310, 2083
BstDEI CTNAG 5 cut(s) 116, 861, 1319, 1403, 1515
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 9 cut(s) 498, 940, 1015, 1375, 1678, 1696, 2005, 2146, 2188
BstMAI GTCTC 2 cut(s) 679, 1403
BstMBI GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
BstMCI CGRYCG 1 cut(s) 1032
BstMWI GCNNNNNNNGC 6 cut(s) 79, 820, 857, 1037, 1254, 1431
BstNSI RCATGY 2 cut(s) 378, 2025
BstSCI CCNGG 1 cut(s) 411
BstSFI CTRYAG 2 cut(s) 1041, 2115
BstV1I GCAGC 6 cut(s) 34, 1269, 1272, 1587, 1745, 1952
BstV2I GAAGAC 1 cut(s) 1409
BstX2I RGATCY 1 cut(s) 2002
BstYI RGATCY 1 cut(s) 2002
BstZI CGGCCG 1 cut(s) 1029
BsuI GTATCC 4 cut(s) 589, 1148, 1322, 1564
BsuRI GGCC 4 cut(s) 1031, 1248, 1291, 1447
BtgZI GCGATG 1 cut(s) 962
BtsI GCAGTG 2 cut(s) 566, 1801
BtsIMutI CAGTG 6 cut(s) 227, 566, 989, 1653, 1801, 1977
Cac8I GCNNGC 3 cut(s) 376, 1310, 2083
CaiI CAGNNNCTG 1 cut(s) 860
CciI TCATGA 1 cut(s) 2038
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 4 cut(s) 965, 1101, 1115, 1247
DdeI CTNAG 5 cut(s) 116, 861, 1319, 1403, 1515
DpnI GATC 9 cut(s) 497, 939, 1014, 1374, 1677, 1695, 2004, 2145, 2187
DpnII GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
DraI TTTAAA 2 cut(s) 639, 1092
DraIII CACNNNGTG 1 cut(s) 1550
DrdI GACNNNNNNGTC 1 cut(s) 1540
DriI GACNNNNNGTC 1 cut(s) 1395
DseDI GACNNNNNNGTC 1 cut(s) 1540
EaeI YGGCCR 1 cut(s) 1029
EagI CGGCCG 1 cut(s) 1029
Eam1104I CTCTTC 3 cut(s) 504, 667, 2163
Eam1105I GACNNNNNGTC 1 cut(s) 1395
EarI CTCTTC 3 cut(s) 504, 667, 2163
Ecl136II GAGCTC 2 cut(s) 322, 1164
EclXI CGGCCG 1 cut(s) 1029
Eco130I CCWWGG 4 cut(s) 339, 1385, 1934, 2053
Eco147I AGGCCT 1 cut(s) 1291
Eco24I GRGCYC 2 cut(s) 324, 1166
Eco47I GGWCC 3 cut(s) 965, 1101, 1115
Eco52I CGGCCG 1 cut(s) 1029
Eco53kI GAGCTC 2 cut(s) 322, 1164
Eco57I CTGAAG 1 cut(s) 1722
Eco88I CYCGRG 1 cut(s) 1368
EcoICRI GAGCTC 2 cut(s) 322, 1164
EcoO109I RGGNCCY 1 cut(s) 1101
EcoRI GAATTC 2 cut(s) 197, 1753
EcoT14I CCWWGG 4 cut(s) 339, 1385, 1934, 2053
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 2 cut(s) 324, 1166
ErhI CCWWGG 4 cut(s) 339, 1385, 1934, 2053
FaqI GGGAC 4 cut(s) 847, 1061, 1114, 1756
FauI CCCGC 1 cut(s) 971
FauNDI CATATG 1 cut(s) 1957
FbaI TGATCA 1 cut(s) 495
Fnu4HI GCNGC 7 cut(s) 23, 1029, 1258, 1261, 1576, 1734, 1966
FriOI GRGCYC 2 cut(s) 324, 1166
Fsp4HI GCNGC 7 cut(s) 23, 1029, 1258, 1261, 1576, 1734, 1966
FspBI CTAG 4 cut(s) 788, 1254, 1293, 2007
GlaI GCGC 1 cut(s) 813
GluI GCNGC 7 cut(s) 23, 1029, 1258, 1261, 1576, 1734, 1966
GsaI CCCAGC 1 cut(s) 2071
GsuI CTGGAG 4 cut(s) 87, 93, 837, 1514
HaeIII GGCC 4 cut(s) 1031, 1248, 1291, 1447
HapII CCGG 2 cut(s) 412, 1661
HhaI GCGC 1 cut(s) 814
Hin1I GRCGYC 1 cut(s) 1490
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 1762
HindII GTYRAC 1 cut(s) 1762
HindIII AAGCTT 1 cut(s) 2131
HinfI GANTC 8 cut(s) 404, 782, 791, 863, 893, 1630, 1664, 1884
HpaI GTTAAC 1 cut(s) 1762
HpaII CCGG 2 cut(s) 412, 1661
HphI GGTGA 1 cut(s) 1536
Hpy166II GTNNAC 4 cut(s) 182, 803, 1714, 1762
Hpy188I TCNGA 8 cut(s) 796, 898, 1140, 1377, 1488, 1600, 1752, 1861
Hpy8I GTNNAC 4 cut(s) 182, 803, 1714, 1762
HpyCH4III ACNGT 2 cut(s) 1657, 2201
HpyCH4IV ACGT 3 cut(s) 144, 1490, 1764
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 820, 857, 1037, 1254, 1431
HpyF3I CTNAG 5 cut(s) 116, 861, 1319, 1403, 1515
HpySE526I ACGT 3 cut(s) 144, 1490, 1764
Hsp92I GRCGYC 1 cut(s) 1490
HspAI GCGC 1 cut(s) 812
Kpn2I TCCGGA 1 cut(s) 1660
KpnI GGTACC 1 cut(s) 1360
Ksp22I TGATCA 1 cut(s) 495
KspAI GTTAAC 1 cut(s) 1762
Kzo9I GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
LmnI GCTCC 4 cut(s) 106, 112, 341, 856
Lsp1109I GCAGC 6 cut(s) 34, 1269, 1272, 1587, 1745, 1952
LweI GCATC 4 cut(s) 55, 274, 939, 965
MaeI CTAG 4 cut(s) 788, 1254, 1293, 2007
MaeII ACGT 3 cut(s) 144, 1490, 1764
MaeIII GTNAC 6 cut(s) 251, 359, 783, 1542, 1651, 1787
MalI GATC 9 cut(s) 497, 939, 1014, 1374, 1677, 1695, 2004, 2145, 2187
MboI GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
MflI RGATCY 1 cut(s) 2002
MhlI GDGCHC 3 cut(s) 324, 338, 1166
MlyI GAGTC 3 cut(s) 791, 872, 1673
Mph1103I ATGCAT 1 cut(s) 376
MroI TCCGGA 1 cut(s) 1660
MroXI GAANNNNTTC 2 cut(s) 1482, 2133
MseI TTAA 7 cut(s) 221, 393, 638, 917, 1091, 1761, 1830
MspI CCGG 2 cut(s) 412, 1661
MspR9I CCNGG 1 cut(s) 413
Mva1269I GAATGC 2 cut(s) 1310, 1516
MwoI GCNNNNNNNGC 6 cut(s) 79, 820, 857, 1037, 1254, 1431
NciI CCSGG 1 cut(s) 413
NdeI CATATG 1 cut(s) 1957
NdeII GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
NlaIV GGNNCC 2 cut(s) 1102, 1358
NmuCI GTSAC 3 cut(s) 783, 1542, 1651
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 2 cut(s) 378, 2025
NspV TTCGAA 1 cut(s) 1849
PaeI GCATGC 1 cut(s) 378
PaeR7I CTCGAG 1 cut(s) 1368
PagI TCATGA 1 cut(s) 2038
PceI AGGCCT 1 cut(s) 1291
PciI ACATGT 1 cut(s) 2021
PcsI WCGNNNNNNNCGW 1 cut(s) 1029
PctI GAATGC 2 cut(s) 1310, 1516
PdmI GAANNNNTTC 2 cut(s) 1482, 2133
PfeI GAWTC 5 cut(s) 404, 791, 893, 1630, 1884
PkrI GCNGC 7 cut(s) 24, 1030, 1259, 1262, 1577, 1735, 1967
PleI GAGTC 3 cut(s) 790, 871, 1672
PpsI GAGTC 3 cut(s) 790, 871, 1672
PpuMI RGGWCCY 1 cut(s) 1101
PscI ACATGT 1 cut(s) 2021
Psp124BI GAGCTC 2 cut(s) 324, 1166
Psp1406I AACGTT 1 cut(s) 1764
Psp5II RGGWCCY 1 cut(s) 1101
PspFI CCCAGC 1 cut(s) 2067
PspN4I GGNNCC 2 cut(s) 1102, 1358
PspPI GGNCC 4 cut(s) 965, 1101, 1115, 1247
PspPPI RGGWCCY 1 cut(s) 1101
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 1 cut(s) 2002
SacI GAGCTC 2 cut(s) 324, 1166
SaqAI TTAA 7 cut(s) 221, 393, 638, 917, 1091, 1761, 1830
SatI GCNGC 7 cut(s) 23, 1029, 1258, 1261, 1576, 1734, 1966
Sau3AI GATC 9 cut(s) 495, 937, 1012, 1372, 1675, 1693, 2002, 2143, 2185
Sau96I GGNCC 4 cut(s) 965, 1101, 1115, 1247
ScaI AGTACT 1 cut(s) 2076
SchI GAGTC 3 cut(s) 791, 872, 1673
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 3 cut(s) 324, 338, 1166
SfaNI GCATC 4 cut(s) 55, 274, 939, 965
SfcI CTRYAG 2 cut(s) 1041, 2115
Sfr274I CTCGAG 1 cut(s) 1368
SfuI TTCGAA 1 cut(s) 1849
SinI GGWCC 3 cut(s) 965, 1101, 1115
SlaI CTCGAG 1 cut(s) 1368
SmiI ATTTAAAT 1 cut(s) 639
SmlI CTYRAG 2 cut(s) 1159, 1368
SmoI CTYRAG 2 cut(s) 1159, 1368
SphI GCATGC 1 cut(s) 378
SseBI AGGCCT 1 cut(s) 1291
SsiI CCGC 2 cut(s) 978, 1028
SspMI CTAG 4 cut(s) 788, 1254, 1293, 2007
SstI GAGCTC 2 cut(s) 324, 1166
StuI AGGCCT 1 cut(s) 1291
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 4 cut(s) 339, 1385, 1934, 2053
SwaI ATTTAAAT 1 cut(s) 639
TaaI ACNGT 2 cut(s) 1657, 2201
TaiI ACGT 3 cut(s) 147, 1493, 1767
TaqI TCGA 8 cut(s) 213, 318, 473, 1369, 1564, 1849, 2142, 2184
TatI WGTACW 4 cut(s) 34, 544, 802, 2074
TauI GCSGC 1 cut(s) 1031
TfiI GAWTC 5 cut(s) 404, 791, 893, 1630, 1884
Tru1I TTAA 7 cut(s) 221, 393, 638, 917, 1091, 1761, 1830
Tru9I TTAA 7 cut(s) 221, 393, 638, 917, 1091, 1761, 1830
TscAI CASTG 6 cut(s) 234, 573, 996, 1660, 1801, 1977
TseFI GTSAC 3 cut(s) 783, 1542, 1651
TseI GCWGC 6 cut(s) 22, 1257, 1260, 1575, 1733, 1965
Tsp45I GTSAC 3 cut(s) 783, 1542, 1651
TspGWI ACGGA 1 cut(s) 1107
TspRI CASTG 6 cut(s) 234, 573, 996, 1660, 1801, 1977
VpaK11BI GGWCC 3 cut(s) 965, 1101, 1115
XapI RAATTY 7 cut(s) 197, 730, 872, 1109, 1337, 1641, 1753
XceI RCATGY 2 cut(s) 378, 2025
XhoI CTCGAG 1 cut(s) 1368
XmnI GAANNNNTTC 2 cut(s) 1482, 2133
XspI CTAG 4 cut(s) 788, 1254, 1293, 2007
ZraI GACGTC 1 cut(s) 1491
ZrmI AGTACT 1 cut(s) 2076
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.