Rh7DG083700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000408
Physical Location & Seq
Forward (+)
6697551 .. 6701566
4016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG083700.1

Sequence Viewer

Length: 1923 bp
ATGTCTATGAGTCTGAACTTTCTGGGAGGAGCACCTTTTCAACTCCTATACGATGGTGTTAGGGTAGCCATAGGTAGAACTACCAAGTTCAAAGCCCTCCTCTTAAATCTCGAATCGACGCTAGACTGTTTGCAACCACGAGTCATCCAGCAGATAAGAGACCATAATGTGGAGCTGAATCTCCCAAACGATATAATTCAAGACCTGCAAGTGAAAATGGATGATGGTGTGGCACTCATTGCCAATTTATCTAGGCTTAGCGTGTGGAACTGTCGCGTATGGGGGGACTGTTGCAACTGCATGAAGCCTAGCTATGCAGACCAACTTGCTGCATTGGATAGGTCTCTTAGACGACTGTTGGAGATACTAAAGCTTGAGCAGATGAGGAACGTGGTGGAGCTCTTGCTTTTGGCAAGGAGAAGTAATGACCGACAAGATGACTTGGAGAGAAGACAGTTGGAAATTCTGAAAGCGCTGCAAGAAACCGGGGATATGTTAAGAGCGCAGCAGGAAGTAATGGAGAGAATTGAAAGGAATGGTGCTGCATCTTCAGACAGAGGAGGGTCAGCTCCTGCTCCTGCTCCTGCTCTAGGAACAGTATTTAGGGTGTTGTTTGATGTTGTTATCAGAGTAAAGGTCAAGAATATGATGTATGAGCGAATCCTCAAAGATTTTGAATCCACTCTTGAATGCTTGAAGCCACTTATAGAAGAGATGGTGGAGTCTAACAAGTTGCTGCATCTTTCAAATGAGGAACAAGAATATTTCATAATGCAAATGGAAAAGGGTGTAGAGTTAATTCACCAGTGCTCTGAGGCTTGCAAGAGAGGAATAAGGTATAAAAAGTACGAATACACCAAGAAACTTCTGGGATTGGATGCATGTCTTCAAAGGCTTTTCATTGAACTCAAAGAGCAGGTAGTAAGGAATGTGAAGGAGGCCTTGGTGCTTATAAGTAACTTGGAAAAAGAGATCACAGAAATTGAAGGGGTTGTTGTGGTACAAAGTGATGAAATTGAGTGTTCGATTCCAGCACCTCAACCCCGATCACCTTTAGTTGGAGAGGATTTACAAAATGTGCAGATAAATCAAGAGGTGATCGAGGAGACATTCGATGCAGTAAGCAACTTAGAAGAGGAGATCAAGGAAATTAGAGAAGTTCATGTGGTACAAAATAATGAAGTACAAAGTAAAGGTCCTCAACCTTCATTGCCTACAGCTGGATCCAGTGTACAAAGTATGCAGGGAACAAGGGATGTGATCAAGGAAAGATGGGACTCGGAAAAGTTGCCGGATGTCAAGCAAATTAAAGGGAAAGGCTTGTTCGAATATCCGGTTACTGGTGAATATGAACTTCCACCATTTCCAGTGGGACTTGCACAGGAAAGAGAAGATCACGCTGATGCATTCAGTAATGCATCATTGGAAGAACCGGTTGATGAATTGGATATGCATGTCAGCTACTTGAAACGGAAGCTTTTACATGGTGGTCACTTAGCCATTGTGCTGACGGGACCTGAAGGATGCGGGAAAACTAGATTGGCAGAAAAGTTTTGTCAAGATGAGGAAGTCATAGATGAATTCAAGAACAATATTTTCTTCGTCCCTGTTTCGGAAAGCCCCAAATTGCAGCTTATTGTTCAAAAACTATATCCAGAGGAGGGTTACAGGAAAATTATGTTGCCAGATGAAAATGGAGTTCATGCAATTCAGTGGCTGCAAGCATTTGTTAAGCATGTAGGACATCATCGTTCTCTGTTGGTCCTAGATGATGTCTGGCCTGGATCTGAATCCCTTCTTGATGAGTTTGATGAATTCAAAAGGTCAAATTTCAAGATTCTGGTGACATCAAGATTCGAATTTCCTAGATTTGGTTCCTCCTATTATGCAATGACGGATGATATACTTAGGGTAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

640

Amino Acids

73.06

Weight (kDa)

5.12

Isoelectric Point (pI)

59.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 137 5e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 195 - 325 5.1e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 485 - 618 1.2e-10 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
Acc36I ACCTGC 2 cut(s) 213, 907
AccB7I CCANNNNNTGG 1 cut(s) 169
AccII CGCG 1 cut(s) 276
AciI CCGC 1 cut(s) 1527
AclWI GGATC 3 cut(s) 1218, 1231, 1792
AcsI RAATTY 5 cut(s) 462, 1580, 1814, 1828, 1859
AcuI CTGAAG 2 cut(s) 534, 1539
AfaI GTAC 5 cut(s) 848, 1002, 1170, 1185, 1233
AfeI AGCGCT 1 cut(s) 474
AfiI CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1220, 1340, 1612, 1661, 1871
AgeI ACCGGT 1 cut(s) 1432
AjnI CCWGG 1 cut(s) 1780
AjuI GAANNNNNNNTTGG 4 cut(s) 926, 958, 1523, 1555
AluBI AGCT 9 cut(s) 175, 312, 373, 400, 569, 1220, 1461, 1477, 1633
AluI AGCT 9 cut(s) 175, 312, 373, 400, 569, 1220, 1461, 1477, 1633
Alw21I GWGCWC 3 cut(s) 34, 402, 812
Alw26I GTCTC 3 cut(s) 153, 348, 1100
AlwI GGATC 3 cut(s) 1218, 1231, 1792
AlwNI CAGNNNCTG 2 cut(s) 572, 1717
Aor51HI AGCGCT 1 cut(s) 474
AoxI GGCC 2 cut(s) 939, 1778
ApeKI GCWGC 7 cut(s) 329, 475, 505, 542, 736, 1630, 1717
ApoI RAATTY 5 cut(s) 462, 1580, 1814, 1828, 1859
ArsI GACNNNNNNTTYG 2 cut(s) 1180, 1212
AsiGI ACCGGT 1 cut(s) 1432
Asp700I GAANNNNTTC 1 cut(s) 1794
AspLEI GCGC 2 cut(s) 475, 505
AspS9I GGNCC 3 cut(s) 1196, 1514, 1762
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 5 cut(s) 794, 1041, 1108, 1355, 1855
AsuII TTCGAA 2 cut(s) 1326, 1857
AvaII GGWCC 3 cut(s) 1196, 1514, 1762
BamHI GGATCC 1 cut(s) 1223
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 457, 878
Bbv12I GWGCWC 3 cut(s) 34, 402, 812
BbvI GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1642, 1704
BccI CCATC 4 cut(s) 47, 218, 709, 1266
BciT130I CCWGG 1 cut(s) 1782
BclI TGATCA 1 cut(s) 1260
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 3 cut(s) 153, 348, 1100
BfaI CTAG 7 cut(s) 122, 252, 309, 590, 1536, 1766, 1866
BfmI CTRYAG 1 cut(s) 1215
BfoI RGCGCY 1 cut(s) 476
BfuAI ACCTGC 2 cut(s) 213, 907
BisI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1631, 1718
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 7 cut(s) 331, 477, 507, 544, 738, 1632, 1719
Bme1390I CCNGG 2 cut(s) 487, 1782
Bme18I GGWCC 3 cut(s) 1196, 1514, 1762
BmgT120I GGNCC 3 cut(s) 1196, 1514, 1762
BmiI GGNNCC 3 cut(s) 1225, 1515, 1876
BmrFI CCNGG 2 cut(s) 487, 1782
BmsI GCATC 7 cut(s) 554, 748, 868, 1105, 1393, 1427, 1514
BpiI GAAGAC 2 cut(s) 457, 878
Bpu1102I GCTNAGC 1 cut(s) 257
Bpu14I TTCGAA 2 cut(s) 1326, 1857
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 1789
BsaI GGTCTC 2 cut(s) 153, 348
BsaJI CCNNGG 2 cut(s) 486, 942
BsaWI WCCGGW 2 cut(s) 1333, 1432
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1220, 1340, 1612, 1661, 1871
Bse118I RCCGGY 1 cut(s) 1432
Bse1I ACTGG 4 cut(s) 805, 1227, 1345, 1367
Bse3DI GCAATG 3 cut(s) 237, 1208, 1896
Bse8I GATNNNNATC 1 cut(s) 1789
BseBI CCWGG 1 cut(s) 1782
BseDI CCNNGG 2 cut(s) 486, 942
BseGI GGATG 7 cut(s) 144, 226, 883, 1261, 1300, 1529, 1903
BseJI GATNNNNATC 1 cut(s) 1789
BseLI CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1220, 1340, 1612, 1661, 1871
BseMI GCAATG 3 cut(s) 237, 1208, 1896
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 4 cut(s) 805, 1227, 1345, 1367
BseRI GAGGAG 6 cut(s) 42, 89, 573, 1118, 1151, 1673
BseXI GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1642, 1704
BsgI GTGCAG 1 cut(s) 1100
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 2 cut(s) 941, 1780
BshTI ACCGGT 1 cut(s) 1432
BsiHKAI GWGCWC 3 cut(s) 34, 402, 812
BsiSI CCGG 4 cut(s) 486, 1292, 1334, 1433
BslFI GGGAC 5 cut(s) 299, 1289, 1386, 1527, 1589
BslI CCNNNNNNNGG 8 cut(s) 169, 590, 1058, 1220, 1340, 1612, 1661, 1871
BsmAI GTCTC 3 cut(s) 153, 348, 1100
BsmFI GGGAC 5 cut(s) 299, 1289, 1386, 1527, 1589
BsmI GAATGC 2 cut(s) 695, 1406
BsnI GGCC 2 cut(s) 941, 1780
Bso31I GGTCTC 2 cut(s) 153, 348
Bsp119I TTCGAA 2 cut(s) 1326, 1857
Bsp1286I GDGCHC 3 cut(s) 34, 402, 812
Bsp1407I TGTACA 1 cut(s) 1231
Bsp143I GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 1 cut(s) 1527
BspANI GGCC 2 cut(s) 941, 1780
BspCNI CTCAG 1 cut(s) 805
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 3 cut(s) 1225, 1515, 1876
BspMI ACCTGC 2 cut(s) 213, 907
BspPI GGATC 3 cut(s) 1218, 1231, 1792
BspT104I TTCGAA 2 cut(s) 1326, 1857
BspTNI GGTCTC 2 cut(s) 153, 348
BsrDI GCAATG 3 cut(s) 237, 1208, 1896
BsrFI RCCGGY 1 cut(s) 1432
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 4 cut(s) 805, 1227, 1345, 1367
BssAI RCCGGY 1 cut(s) 1432
BssECI CCNNGG 2 cut(s) 486, 942
BssMI GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 942
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 1782
Bst4CI ACNGT 6 cut(s) 128, 272, 290, 357, 456, 598
Bst6I CTCTTC 2 cut(s) 705, 1128
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 1231
BstBI TTCGAA 2 cut(s) 1326, 1857
BstC8I GCNNGC 2 cut(s) 820, 1722
BstDEI CTNAG 6 cut(s) 257, 347, 813, 1129, 1495, 1907
BstENI CCTNNNNNAGG 1 cut(s) 588
BstF5I GGATG 7 cut(s) 144, 226, 883, 1261, 1300, 1529, 1903
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 2 cut(s) 475, 505
BstKTI GATC 8 cut(s) 975, 1049, 1101, 1143, 1226, 1263, 1396, 1787
BstMAI GTCTC 3 cut(s) 153, 348, 1100
BstMBI GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 1782
BstNSI RCATGY 3 cut(s) 885, 1457, 1739
BstSCI CCNGG 2 cut(s) 485, 1780
BstSFI CTRYAG 1 cut(s) 1215
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1642, 1704
BstV2I GAAGAC 2 cut(s) 457, 878
BstX2I RGATCY 2 cut(s) 1223, 1784
BstYI RGATCY 2 cut(s) 1223, 1784
BsuRI GGCC 2 cut(s) 941, 1780
BtsCI GGATG 7 cut(s) 144, 226, 883, 1261, 1300, 1529, 1903
BtsIMutI CAGTG 4 cut(s) 812, 1234, 1374, 1718
BveI ACCTGC 2 cut(s) 213, 907
Cac8I GCNNGC 2 cut(s) 820, 1722
CaiI CAGNNNCTG 2 cut(s) 572, 1717
CfoI GCGC 2 cut(s) 475, 505
Cfr10I RCCGGY 1 cut(s) 1432
Cfr13I GGNCC 3 cut(s) 1196, 1514, 1762
CseI GACGC 1 cut(s) 127
Csp6I GTAC 5 cut(s) 847, 1001, 1169, 1184, 1232
CspAI ACCGGT 1 cut(s) 1432
CviAII CATG 7 cut(s) 301, 882, 1163, 1454, 1484, 1703, 1736
CviQI GTAC 5 cut(s) 847, 1001, 1169, 1184, 1232
DdeI CTNAG 6 cut(s) 257, 347, 813, 1129, 1495, 1907
DpnI GATC 8 cut(s) 974, 1048, 1100, 1142, 1225, 1262, 1395, 1786
DpnII GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
Eam1104I CTCTTC 2 cut(s) 705, 1128
EarI CTCTTC 2 cut(s) 705, 1128
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 942
Eco147I AGGCCT 1 cut(s) 941
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 2 cut(s) 153, 348
Eco47I GGWCC 3 cut(s) 1196, 1514, 1762
Eco47III AGCGCT 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 534, 1539
EcoICRI GAGCTC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 588
EcoO109I RGGNCCY 2 cut(s) 1196, 1514
EcoRI GAATTC 2 cut(s) 1580, 1814
EcoRII CCWGG 1 cut(s) 1780
EcoT14I CCWWGG 1 cut(s) 942
EcoT22I ATGCAT 4 cut(s) 883, 1408, 1420, 1455
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 942
FaeI CATG 7 cut(s) 304, 885, 1166, 1457, 1487, 1706, 1739
FalI AAGNNNNNCTT 2 cut(s) 926, 958
FaqI GGGAC 5 cut(s) 299, 1289, 1386, 1527, 1589
FatI CATG 7 cut(s) 300, 881, 1162, 1453, 1483, 1702, 1735
FauI CCCGC 1 cut(s) 1520
FbaI TGATCA 1 cut(s) 1260
Fnu4HI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1631, 1718
FokI GGATG 7 cut(s) 131, 233, 890, 1268, 1307, 1536, 1910
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1631, 1718
FspBI CTAG 7 cut(s) 122, 252, 309, 590, 1536, 1766, 1866
GlaI GCGC 2 cut(s) 474, 504
GluI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1631, 1718
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 2 cut(s) 941, 1780
HapII CCGG 4 cut(s) 486, 1292, 1334, 1433
HgaI GACGC 1 cut(s) 127
HhaI GCGC 2 cut(s) 475, 505
Hin1II CATG 7 cut(s) 304, 885, 1166, 1457, 1487, 1706, 1739
Hin6I GCGC 2 cut(s) 473, 503
HinP1I GCGC 2 cut(s) 473, 503
HindIII AAGCTT 2 cut(s) 371, 1475
HpaII CCGG 4 cut(s) 486, 1292, 1334, 1433
HphI GGTGA 5 cut(s) 794, 1041, 1108, 1355, 1855
Hpy166II GTNNAC 1 cut(s) 1232
Hpy188I TCNGA 8 cut(s) 15, 468, 553, 629, 814, 1282, 1615, 1789
Hpy8I GTNNAC 1 cut(s) 1232
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 5 cut(s) 928, 980, 1215, 1514, 1805
HpyCH4III ACNGT 6 cut(s) 128, 272, 290, 357, 456, 598
HpyCH4IV ACGT 1 cut(s) 390
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 6 cut(s) 257, 347, 813, 1129, 1495, 1907
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 7 cut(s) 304, 885, 1166, 1457, 1487, 1706, 1739
HspAI GCGC 2 cut(s) 473, 503
Ksp22I TGATCA 1 cut(s) 1260
Kzo9I GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
LmnI GCTCC 6 cut(s) 29, 172, 397, 574, 580, 586
Lsp1109I GCAGC 7 cut(s) 316, 462, 517, 529, 723, 1642, 1704
LweI GCATC 7 cut(s) 554, 748, 868, 1105, 1393, 1427, 1514
MaeI CTAG 7 cut(s) 122, 252, 309, 590, 1536, 1766, 1866
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 5 cut(s) 956, 1336, 1490, 1664, 1843
MalI GATC 8 cut(s) 974, 1048, 1100, 1142, 1225, 1262, 1395, 1786
MboI GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
MboII GAAGA 8 cut(s) 462, 540, 722, 878, 1145, 1403, 1439, 1591
MflI RGATCY 2 cut(s) 1223, 1784
MhlI GDGCHC 3 cut(s) 34, 402, 812
MlyI GAGTC 4 cut(s) 19, 150, 731, 1271
MmeI TCCRAC 3 cut(s) 339, 438, 1039
Mph1103I ATGCAT 4 cut(s) 883, 1408, 1420, 1455
MroXI GAANNNNTTC 1 cut(s) 1794
MseI TTAA 5 cut(s) 104, 497, 797, 1308, 1731
MslI CAYNNNNRTG 1 cut(s) 1401
MspA1I CMGCKG 1 cut(s) 1220
MspI CCGG 4 cut(s) 486, 1292, 1334, 1433
MspR9I CCNGG 2 cut(s) 487, 1782
Mva1269I GAATGC 2 cut(s) 695, 1406
MvaI CCWGG 1 cut(s) 1782
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 487
NdeII GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
NlaIII CATG 7 cut(s) 304, 885, 1166, 1457, 1487, 1706, 1739
NlaIV GGNNCC 3 cut(s) 1225, 1515, 1876
NmuCI GTSAC 2 cut(s) 1490, 1843
NsiI ATGCAT 4 cut(s) 883, 1408, 1420, 1455
NspI RCATGY 3 cut(s) 885, 1457, 1739
NspV TTCGAA 2 cut(s) 1326, 1857
PceI AGGCCT 1 cut(s) 941
PctI GAATGC 2 cut(s) 695, 1406
PdmI GAANNNNTTC 1 cut(s) 1794
PfeI GAWTC 8 cut(s) 113, 178, 660, 677, 1027, 1790, 1837, 1854
PflMI CCANNNNNTGG 1 cut(s) 169
PinAI ACCGGT 1 cut(s) 1432
PkrI GCNGC 7 cut(s) 331, 477, 507, 544, 738, 1632, 1719
PleI GAGTC 4 cut(s) 18, 149, 730, 1271
PpsI GAGTC 4 cut(s) 18, 149, 730, 1271
PpuMI RGGWCCY 2 cut(s) 1196, 1514
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 2 cut(s) 1196, 1514
Psp6I CCWGG 1 cut(s) 1780
PspGI CCWGG 1 cut(s) 1780
PspN4I GGNNCC 3 cut(s) 1225, 1515, 1876
PspPI GGNCC 3 cut(s) 1196, 1514, 1762
PspPPI RGGWCCY 2 cut(s) 1196, 1514
PstNI CAGNNNCTG 2 cut(s) 572, 1717
PsuI RGATCY 2 cut(s) 1223, 1784
PvuII CAGCTG 1 cut(s) 1220
RsaI GTAC 5 cut(s) 848, 1002, 1170, 1185, 1233
RsaNI GTAC 5 cut(s) 847, 1001, 1169, 1184, 1232
RseI CAYNNNNRTG 1 cut(s) 1401
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 5 cut(s) 104, 497, 797, 1308, 1731
SatI GCNGC 7 cut(s) 330, 476, 506, 543, 737, 1631, 1718
Sau3AI GATC 8 cut(s) 972, 1046, 1098, 1140, 1223, 1260, 1393, 1784
Sau96I GGNCC 3 cut(s) 1196, 1514, 1762
SchI GAGTC 4 cut(s) 19, 150, 731, 1271
ScrFI CCNGG 2 cut(s) 487, 1782
SduI GDGCHC 3 cut(s) 34, 402, 812
SfaNI GCATC 7 cut(s) 554, 748, 868, 1105, 1393, 1427, 1514
SfcI CTRYAG 1 cut(s) 1215
SfuI TTCGAA 2 cut(s) 1326, 1857
SinI GGWCC 3 cut(s) 1196, 1514, 1762
SmiMI CAYNNNNRTG 1 cut(s) 1401
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SseBI AGGCCT 1 cut(s) 941
SsiI CCGC 1 cut(s) 1527
SspI AATATT 2 cut(s) 764, 1594
SspMI CTAG 7 cut(s) 122, 252, 309, 590, 1536, 1766, 1866
SstI GAGCTC 1 cut(s) 402
StuI AGGCCT 1 cut(s) 941
StyD4I CCNGG 2 cut(s) 485, 1780
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 6 cut(s) 128, 272, 290, 357, 456, 598
TaiI ACGT 1 cut(s) 393
TaqI TCGA 7 cut(s) 111, 116, 1025, 1101, 1113, 1326, 1857
TaqII GACCGA 1 cut(s) 444
TatI WGTACW 2 cut(s) 1183, 1231
TfiI GAWTC 8 cut(s) 113, 178, 660, 677, 1027, 1790, 1837, 1854
Tru1I TTAA 5 cut(s) 104, 497, 797, 1308, 1731
Tru9I TTAA 5 cut(s) 104, 497, 797, 1308, 1731
TscAI CASTG 4 cut(s) 812, 1234, 1374, 1718
TseFI GTSAC 2 cut(s) 1490, 1843
TseI GCWGC 7 cut(s) 329, 475, 505, 542, 736, 1630, 1717
Tsp45I GTSAC 2 cut(s) 1490, 1843
TspGWI ACGGA 2 cut(s) 1486, 1910
TspRI CASTG 4 cut(s) 812, 1234, 1374, 1718
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 3 cut(s) 1196, 1514, 1762
XagI CCTNNNNNAGG 1 cut(s) 588
XapI RAATTY 5 cut(s) 462, 1580, 1814, 1828, 1859
XceI RCATGY 3 cut(s) 885, 1457, 1739
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XmnI GAANNNNTTC 1 cut(s) 1794
XspI CTAG 7 cut(s) 122, 252, 309, 590, 1536, 1766, 1866
Zsp2I ATGCAT 4 cut(s) 883, 1408, 1420, 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.