Rorug06G0475600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000408
Physical Location & Seq
Reverse (-)
59795257 .. 59800634
5378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0475600.1

Sequence Viewer

Length: 1950 bp
ATGGCTCAGATTCAAGTTCAGCATCAGGCTCCGGTGACCGGTCCGAACGGCGTCGCTGTCAACGCCGGTGGTCAGCAACAGATGGTGTCCACGTCGCTTTACGTCGGGGATCTGGAGCAGAATGTGACCGACTCGCAGCTCTACGATCTGTTCAATCAGGTCGGCCAGGTCGTCTCCGTTAGGGTTTGCAGGGACTTGTCCACTCGCAGATCTCTTGGTTATGGCTATGTTAATTATAGCAACCCTCAGGATGCTGCGAGGGCACTAGAAGTACTGAACTTCACTCCACTGAATGGCAAATCTATCAGAATTTGCTATTCTCTTCGGGACCCTACTATTAGAAAGAGTGGTACTGGGAATATTTTTATCAAGAATCTGGACAAGGGAATTGACCACAAGGCATTGCATGATACATTCTCGTCTTTTGGCAACATACTTTCTTGCAAGATAGCTACGGATGCTTCTGGGCAGTCCAAGGGCTATGGTTTTGTTCAATTTGACAAGGAAGAAGCTGCTGAGAGTGCCATAGAGAAGTTAAATGGCATGCTGTTAAATGATAAACAAGTGTACGTAGGTCCCTTTCTCCGTAGGCAGGAGAGGGATGGTGCTTTGCAGAAGACAAAATTTAGCAATGTCTACGTGAAAAATATATCAGAATCTACTACAGATGATGACTTGAAGAAAATTTTTGGTGAACATGGACCCATTACCAGTGCTGTAGTGATGAGGGATGGTGATGGCAAATCAAGGGGTTTTGGTTTTGTCAACTTTGATAACCCAGATGATGCTGCTAAAGCCGTTGAGCTTCTGAATGGAAAGCAATTTGATGATAAGGAATGGTATGTTGGCAAAGCCCAGAAGAAGACTGAAAGAGAAGTTGAACTGAAGGAGAAATTTGAGCACAGCATGAAGGAAGCTAGTGATAAATTTCAAGGTGTGAATTTGTATATCAAGAACTTGGATGATAGCATTGATGATGAGAAACTTAAGGAAATATTCTCCGAGTATGGTACGGTCACATCTTGCAAGGTTATGCGTGACCCTGAAGGAGTTAGTCGTGGTTCTGGTTTTGTTGCCTTCTCGACTTCTGAAGAAGCTTCTCGAGCTCTTACGGAGATGAATGGCAAAATGGTTGTTAGCAAGCCTCTTTATGTTGCACTTGCACAACGGAAGGAAGAGAGAAGGGCAAGGTTGCAGGCTCAGTTTTCACAAATGAGGCCTGTTGCTATGGCTCCTTCAGTGGCTCCTCGTTTACCAATGTACCCTCCTGGTGCTCCAGGTCTAGGGCAACAATTTATGTATGGGCAAGGGCCTCCAACCATAATTCCACAGCAAGCTGGATTTGGTTACCAACAGCAACTTGTGCCAGGATTGAGGCCTGGAGGTGCTCCTATGCCAAACTTCTTTGTTCCAATGGTCCAACAGGGTCAGCAAGGCCAACGCCCAGGAGGTCGACGTGGGGCAGGTCCTGTGCAACAAAACCAGCAGCAAGTGCCACTGATGCCACAGCACATGATTCCAAGGGGACGTATGTATCGCTTTCCACCTGGTCGCAATATGCCGGAAGCTCCATTTTCTGGTGGTATGCTCTCAGTTCCATATGACATGGGTGGCATGCCTATGCGTGATCCTGCAGGGGGACGTGCTATGCCTGTTCCTGCTTTGGCTTCATCCCTTGCAAATGCTACACCAGAAGAACAGAGGACGATGCTTGGTGAAGCTTTATATCCCCAAGTTGATGCTTTGGAGCCCGACTCAGCAGCCAAGGTTACAGGCATGCTTCTGGAGATGGACCAGACTGAGGTTTTGCATTTGCTCGAGTCACCTGAGGCTCTGAAGGCAAAGGTTGCAGAGGCAATAGAGGTCCTGAGGAATGTTACCCAACATCAGGTTAACAACAATGCTGATCAGTTAGCATCAATATCTCTGACTGACAAGCTTGTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

649

Amino Acids

71.21

Weight (kDa)

6.75

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 33 - 103 1.5e-19 RNA recognition motif
RRM_1 PF00076 121 - 189 2.2e-21 RNA recognition motif
RRM_1 PF00076 212 - 280 9.6e-24 RNA recognition motif
RRM_1 PF00076 315 - 383 1.2e-19 RNA recognition motif
MLLE PF00658 559 - 623 4.3e-29 MLLE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1454
AccB7I CCANNNNNTGG 2 cut(s) 293, 1577
AccI GTMKAC 2 cut(s) 636, 1453
AclWI GGATC 2 cut(s) 117, 1622
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 6 cut(s) 309, 623, 684, 893, 926, 940
AcuI CTGAAG 5 cut(s) 905, 1065, 1110, 1221, 1856
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 5 cut(s) 273, 352, 569, 1012, 1262
AfiI CCNNNNNNNGG 8 cut(s) 38, 293, 592, 1283, 1577, 1637, 1801, 1888
AflII CTTAAG 1 cut(s) 986
AgeI ACCGGT 1 cut(s) 38
AgsI TTSAA 6 cut(s) 14, 154, 494, 679, 881, 932
AjiI CACGTC 3 cut(s) 93, 1457, 1643
AjnI CCWGG 7 cut(s) 165, 1267, 1276, 1366, 1378, 1444, 1546
AjuI GAANNNNNNNTTGG 2 cut(s) 828, 860
Alw21I GWGCWC 4 cut(s) 903, 1108, 1276, 1390
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 2 cut(s) 117, 1622
AlwNI CAGNNNCTG 1 cut(s) 1469
Ama87I CYCGRG 2 cut(s) 1101, 1817
AoxI GGCC 5 cut(s) 163, 1217, 1310, 1376, 1435
ApeKI GCWGC 6 cut(s) 136, 254, 512, 788, 1486, 1760
ApoI RAATTY 6 cut(s) 309, 623, 684, 893, 926, 940
ArsI GACNNNNNNTTYG 2 cut(s) 1789, 1821
AsiGI ACCGGT 1 cut(s) 38
AspS9I GGNCC 9 cut(s) 41, 328, 575, 701, 1310, 1417, 1466, 1792, 1864
AsuHPI GGTGA 5 cut(s) 46, 704, 746, 1727, 1815
AvaI CYCGRG 2 cut(s) 1101, 1817
AvaII GGWCC 8 cut(s) 41, 328, 575, 701, 1417, 1466, 1792, 1864
AxyI CCTNAGG 3 cut(s) 246, 1827, 1868
BaeGI GKGCMC 1 cut(s) 265
BaeI ACNNNNGTAYC 4 cut(s) 1244, 1277, 1517, 1550
BanII GRGCYC 2 cut(s) 1108, 1752
BbsI GAAGAC 2 cut(s) 623, 869
Bbv12I GWGCWC 4 cut(s) 903, 1108, 1276, 1390
BbvI GCAGC 6 cut(s) 148, 241, 499, 775, 1498, 1772
BccI CCATC 5 cut(s) 76, 596, 725, 731, 1783
BceAI ACGGC 2 cut(s) 64, 782
BciT130I CCWGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
BclI TGATCA 1 cut(s) 1906
BcoDI GTCTC 1 cut(s) 178
BfaI CTAG 3 cut(s) 266, 918, 1283
BfmI CTRYAG 3 cut(s) 663, 717, 1632
BfrI CTTAAG 1 cut(s) 986
BfuAI ACCTGC 1 cut(s) 1454
BglII AGATCT 1 cut(s) 209
BisI GCNGC 6 cut(s) 137, 255, 513, 789, 1487, 1761
BlsI GCNGC 6 cut(s) 138, 256, 514, 790, 1488, 1762
BmcAI AGTACT 1 cut(s) 273
Bme1390I CCNGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
Bme18I GGWCC 8 cut(s) 41, 328, 575, 701, 1417, 1466, 1792, 1864
BmeT110I CYCGRG 2 cut(s) 1101, 1817
BmgBI CACGTC 3 cut(s) 93, 1457, 1643
BmgT120I GGNCC 9 cut(s) 41, 328, 575, 701, 1310, 1417, 1466, 1792, 1864
BmiI GGNNCC 8 cut(s) 30, 329, 330, 577, 703, 1233, 1245, 1749
BmrFI CCNGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 8 cut(s) 31, 241, 448, 775, 1491, 1698, 1729, 1925
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 2 cut(s) 623, 869
BplI GAGNNNNNCTC 2 cut(s) 1739, 1771
BpmI CTGGAG 4 cut(s) 134, 1260, 1401, 1805
BsaAI YACGTR 2 cut(s) 571, 640
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 4 cut(s) 474, 1444, 1520, 1764
BsaWI WCCGGW 2 cut(s) 31, 38
Bsc4I CCNNNNNNNGG 8 cut(s) 38, 293, 592, 1283, 1577, 1637, 1801, 1888
Bse118I RCCGGY 2 cut(s) 38, 65
Bse1I ACTGG 2 cut(s) 358, 711
Bse21I CCTNAGG 3 cut(s) 246, 1827, 1868
Bse3DI GCAATG 2 cut(s) 401, 637
BseBI CCWGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
BseDI CCNNGG 4 cut(s) 474, 1444, 1520, 1764
BseGI GGATG 6 cut(s) 256, 463, 607, 736, 967, 1670
BseLI CCNNNNNNNGG 8 cut(s) 38, 293, 592, 1283, 1577, 1637, 1801, 1888
BseMI GCAATG 2 cut(s) 401, 637
BseMII CTCAG 9 cut(s) 20, 260, 507, 1214, 1605, 1770, 1791, 1818, 1859
BseNI ACTGG 2 cut(s) 358, 711
BseRI GAGGAG 1 cut(s) 1236
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 6 cut(s) 148, 241, 499, 775, 1498, 1772
BshFI GGCC 5 cut(s) 165, 1219, 1312, 1378, 1437
BshTI ACCGGT 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 903, 1108, 1276, 1390
BsiHKCI CYCGRG 2 cut(s) 1101, 1817
BsiSI CCGG 4 cut(s) 32, 39, 66, 1562
BslFI GGGAC 5 cut(s) 206, 341, 561, 1539, 1653
BslI CCNNNNNNNGG 8 cut(s) 38, 293, 592, 1283, 1577, 1637, 1801, 1888
BsmAI GTCTC 1 cut(s) 178
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 5 cut(s) 206, 341, 561, 1539, 1653
BsnI GGCC 5 cut(s) 165, 1219, 1312, 1378, 1437
BsoBI CYCGRG 2 cut(s) 1101, 1817
Bsp1286I GDGCHC 6 cut(s) 265, 903, 1108, 1276, 1390, 1752
Bsp143I GATC 5 cut(s) 109, 145, 209, 1627, 1906
BspANI GGCC 5 cut(s) 165, 1219, 1312, 1378, 1437
BspCNI CTCAG 9 cut(s) 19, 259, 508, 1213, 1604, 1769, 1792, 1819, 1860
BspLI GGNNCC 8 cut(s) 30, 329, 330, 577, 703, 1233, 1245, 1749
BspMAI CTGCAG 1 cut(s) 1636
BspMI ACCTGC 1 cut(s) 1454
BspPI GGATC 2 cut(s) 117, 1622
BspTI CTTAAG 1 cut(s) 986
BsrDI GCAATG 2 cut(s) 401, 637
BsrFI RCCGGY 2 cut(s) 38, 65
BsrI ACTGG 2 cut(s) 358, 711
BssAI RCCGGY 2 cut(s) 38, 65
BssECI CCNNGG 4 cut(s) 474, 1444, 1520, 1764
BssMI GATC 5 cut(s) 109, 145, 209, 1627, 1906
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 3 cut(s) 474, 1520, 1764
Bst2UI CCWGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
Bst4CI ACNGT 1 cut(s) 1015
Bst6I CTCTTC 2 cut(s) 327, 1170
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 986
BstAPI GCANNNNNTGC 3 cut(s) 1363, 1492, 1847
BstBAI YACGTR 2 cut(s) 571, 640
BstC8I GCNNGC 6 cut(s) 545, 1142, 1197, 1335, 1616, 1778
BstDEI CTNAG 9 cut(s) 6, 246, 516, 1200, 1591, 1756, 1800, 1827, 1868
BstEII GGTNACC 2 cut(s) 34, 1346
BstF5I GGATG 6 cut(s) 256, 463, 607, 736, 967, 1670
BstKTI GATC 5 cut(s) 112, 148, 212, 1630, 1909
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 5 cut(s) 109, 145, 209, 1627, 1906
BstNI CCWGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
BstNSI RCATGY 3 cut(s) 547, 1618, 1780
BstPI GGTNACC 2 cut(s) 34, 1346
BstSCI CCNGG 7 cut(s) 165, 1267, 1276, 1366, 1378, 1444, 1546
BstSFI CTRYAG 3 cut(s) 663, 717, 1632
BstSLI GKGCMC 1 cut(s) 265
BstSNI TACGTA 1 cut(s) 571
BstV1I GCAGC 6 cut(s) 148, 241, 499, 775, 1498, 1772
BstV2I GAAGAC 2 cut(s) 623, 869
BstX2I RGATCY 2 cut(s) 109, 209
BstYI RGATCY 2 cut(s) 109, 209
Bsu36I CCTNAGG 3 cut(s) 246, 1827, 1868
BsuRI GGCC 5 cut(s) 165, 1219, 1312, 1378, 1437
BtrI CACGTC 3 cut(s) 93, 1457, 1643
BtsCI GGATG 6 cut(s) 256, 463, 607, 736, 967, 1670
BtsIMutI CAGTG 4 cut(s) 287, 718, 1245, 1496
BveI ACCTGC 1 cut(s) 1454
Cac8I GCNNGC 6 cut(s) 545, 1142, 1197, 1335, 1616, 1778
CaiI CAGNNNCTG 1 cut(s) 1469
Cfr10I RCCGGY 2 cut(s) 38, 65
Cfr13I GGNCC 9 cut(s) 41, 328, 575, 701, 1310, 1417, 1466, 1792, 1864
CpoI CGGWCCG 1 cut(s) 41
CseI GACGC 1 cut(s) 40
CsiI ACCWGGT 1 cut(s) 1546
Csp6I GTAC 5 cut(s) 272, 351, 568, 1011, 1261
CspAI ACCGGT 1 cut(s) 38
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CspI CGGWCCG 1 cut(s) 41
CviAII CATG 8 cut(s) 407, 544, 698, 907, 1513, 1606, 1615, 1777
CviQI GTAC 5 cut(s) 272, 351, 568, 1011, 1261
DdeI CTNAG 9 cut(s) 6, 246, 516, 1200, 1591, 1756, 1800, 1827, 1868
DpnI GATC 5 cut(s) 111, 147, 211, 1629, 1908
DpnII GATC 5 cut(s) 109, 145, 209, 1627, 1906
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 327, 1170
EarI CTCTTC 2 cut(s) 327, 1170
Ecl136II GAGCTC 1 cut(s) 1106
Eco105I TACGTA 1 cut(s) 571
Eco130I CCWWGG 3 cut(s) 474, 1520, 1764
Eco147I AGGCCT 2 cut(s) 1219, 1378
Eco24I GRGCYC 2 cut(s) 1108, 1752
Eco47I GGWCC 8 cut(s) 41, 328, 575, 701, 1417, 1466, 1792, 1864
Eco53kI GAGCTC 1 cut(s) 1106
Eco57I CTGAAG 5 cut(s) 905, 1065, 1110, 1221, 1856
Eco81I CCTNAGG 3 cut(s) 246, 1827, 1868
Eco88I CYCGRG 2 cut(s) 1101, 1817
Eco91I GGTNACC 2 cut(s) 34, 1346
EcoICRI GAGCTC 1 cut(s) 1106
EcoO109I RGGNCCY 5 cut(s) 328, 575, 1310, 1466, 1864
EcoO65I GGTNACC 2 cut(s) 34, 1346
EcoRII CCWGG 7 cut(s) 165, 1267, 1276, 1366, 1378, 1444, 1546
EcoT14I CCWWGG 3 cut(s) 474, 1520, 1764
EcoT38I GRGCYC 2 cut(s) 1108, 1752
ErhI CCWWGG 3 cut(s) 474, 1520, 1764
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 8 cut(s) 410, 547, 701, 910, 1516, 1609, 1618, 1780
FaqI GGGAC 5 cut(s) 206, 341, 561, 1539, 1653
FatI CATG 8 cut(s) 406, 543, 697, 906, 1512, 1605, 1614, 1776
FauNDI CATATG 1 cut(s) 1600
FbaI TGATCA 1 cut(s) 1906
FblI GTMKAC 2 cut(s) 636, 1453
Fnu4HI GCNGC 6 cut(s) 137, 255, 513, 789, 1487, 1761
FokI GGATG 6 cut(s) 263, 470, 614, 743, 974, 1657
FriOI GRGCYC 2 cut(s) 1108, 1752
Fsp4HI GCNGC 6 cut(s) 137, 255, 513, 789, 1487, 1761
FspBI CTAG 3 cut(s) 266, 918, 1283
GluI GCNGC 6 cut(s) 137, 255, 513, 789, 1487, 1761
GsuI CTGGAG 4 cut(s) 134, 1260, 1401, 1805
HaeIII GGCC 5 cut(s) 165, 1219, 1312, 1378, 1437
HapII CCGG 4 cut(s) 32, 39, 66, 1562
HgaI GACGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 8 cut(s) 410, 547, 701, 910, 1516, 1609, 1618, 1780
HincII GTYRAC 4 cut(s) 61, 766, 1454, 1894
HindII GTYRAC 4 cut(s) 61, 766, 1454, 1894
HindIII AAGCTT 3 cut(s) 1095, 1719, 1937
HinfI GANTC 7 cut(s) 10, 131, 373, 656, 1516, 1754, 1820
HpaI GTTAAC 1 cut(s) 1894
HpaII CCGG 4 cut(s) 32, 39, 66, 1562
HphI GGTGA 5 cut(s) 46, 704, 746, 1727, 1815
Hpy188I TCNGA 9 cut(s) 9, 45, 308, 655, 810, 1003, 1090, 1836, 1929
Hpy99I CGWCG 4 cut(s) 56, 97, 107, 1458
HpyAV CCTTC 8 cut(s) 880, 904, 1040, 1087, 1165, 1176, 1245, 1831
HpyCH4III ACNGT 1 cut(s) 1015
HpyCH4IV ACGT 7 cut(s) 92, 102, 570, 639, 1456, 1528, 1642
HpyF3I CTNAG 9 cut(s) 6, 246, 516, 1200, 1591, 1756, 1800, 1827, 1868
HpySE526I ACGT 7 cut(s) 92, 102, 570, 639, 1456, 1528, 1642
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 8 cut(s) 410, 547, 701, 910, 1516, 1609, 1618, 1780
KflI GGGWCCC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 1906
KspAI GTTAAC 1 cut(s) 1894
Kzo9I GATC 5 cut(s) 109, 145, 209, 1627, 1906
LmnI GCTCC 8 cut(s) 34, 115, 1237, 1249, 1279, 1393, 1573, 1747
Lsp1109I GCAGC 6 cut(s) 148, 241, 499, 775, 1498, 1772
LweI GCATC 8 cut(s) 31, 241, 448, 775, 1491, 1698, 1729, 1925
MabI ACCWGGT 1 cut(s) 1546
MaeI CTAG 3 cut(s) 266, 918, 1283
MaeII ACGT 7 cut(s) 92, 102, 570, 639, 1456, 1528, 1642
MaeIII GTNAC 8 cut(s) 34, 124, 1015, 1037, 1346, 1768, 1821, 1876
MalI GATC 5 cut(s) 111, 147, 211, 1629, 1908
MboI GATC 5 cut(s) 109, 145, 209, 1627, 1906
MboII GAAGA 9 cut(s) 314, 518, 628, 691, 871, 874, 1103, 1187, 1706
MflI RGATCY 2 cut(s) 109, 209
MhlI GDGCHC 6 cut(s) 265, 903, 1108, 1276, 1390, 1752
MlyI GAGTC 3 cut(s) 125, 1748, 1829
MmeI TCCRAC 2 cut(s) 1340, 1444
MseI TTAA 6 cut(s) 231, 536, 551, 987, 1893, 1948
MslI CAYNNNNRTG 1 cut(s) 1619
MspCI CTTAAG 1 cut(s) 986
MspI CCGG 4 cut(s) 32, 39, 66, 1562
MspR9I CCNGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
MvaI CCWGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
NdeI CATATG 1 cut(s) 1600
NdeII GATC 5 cut(s) 109, 145, 209, 1627, 1906
NlaIII CATG 8 cut(s) 410, 547, 701, 910, 1516, 1609, 1618, 1780
NlaIV GGNNCC 8 cut(s) 30, 329, 330, 577, 703, 1233, 1245, 1749
NmuCI GTSAC 5 cut(s) 34, 124, 1015, 1037, 1821
NspI RCATGY 3 cut(s) 547, 1618, 1780
PaeI GCATGC 3 cut(s) 547, 1618, 1780
PaeR7I CTCGAG 2 cut(s) 1101, 1817
PceI AGGCCT 2 cut(s) 1219, 1378
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PfeI GAWTC 4 cut(s) 10, 373, 656, 1516
PflMI CCANNNNNTGG 2 cut(s) 293, 1577
PinAI ACCGGT 1 cut(s) 38
PkrI GCNGC 6 cut(s) 138, 256, 514, 790, 1488, 1762
PleI GAGTC 3 cut(s) 125, 1748, 1828
PpsI GAGTC 3 cut(s) 125, 1748, 1828
Ppu21I YACGTR 2 cut(s) 571, 640
PpuMI RGGWCCY 4 cut(s) 328, 575, 1466, 1864
Psp124BI GAGCTC 1 cut(s) 1108
Psp5II RGGWCCY 4 cut(s) 328, 575, 1466, 1864
Psp6I CCWGG 7 cut(s) 165, 1267, 1276, 1366, 1378, 1444, 1546
PspEI GGTNACC 2 cut(s) 34, 1346
PspGI CCWGG 7 cut(s) 165, 1267, 1276, 1366, 1378, 1444, 1546
PspN4I GGNNCC 8 cut(s) 30, 329, 330, 577, 703, 1233, 1245, 1749
PspPI GGNCC 9 cut(s) 41, 328, 575, 701, 1310, 1417, 1466, 1792, 1864
PspPPI RGGWCCY 4 cut(s) 328, 575, 1466, 1864
PspXI VCTCGAGB 1 cut(s) 1817
PstI CTGCAG 1 cut(s) 1636
PstNI CAGNNNCTG 1 cut(s) 1469
PsuI RGATCY 2 cut(s) 109, 209
RsaI GTAC 5 cut(s) 273, 352, 569, 1012, 1262
RsaNI GTAC 5 cut(s) 272, 351, 568, 1011, 1261
RseI CAYNNNNRTG 1 cut(s) 1619
Rsr2I CGGWCCG 1 cut(s) 41
RsrII CGGWCCG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 1108
SalI GTCGAC 1 cut(s) 1452
SaqAI TTAA 6 cut(s) 231, 536, 551, 987, 1893, 1948
SatI GCNGC 6 cut(s) 137, 255, 513, 789, 1487, 1761
Sau3AI GATC 5 cut(s) 109, 145, 209, 1627, 1906
Sau96I GGNCC 9 cut(s) 41, 328, 575, 701, 1310, 1417, 1466, 1792, 1864
SbfI CCTGCAGG 1 cut(s) 1636
ScaI AGTACT 1 cut(s) 273
SchI GAGTC 3 cut(s) 125, 1748, 1829
ScrFI CCNGG 7 cut(s) 167, 1269, 1278, 1368, 1380, 1446, 1548
SdaI CCTGCAGG 1 cut(s) 1636
SduI GDGCHC 6 cut(s) 265, 903, 1108, 1276, 1390, 1752
SexAI ACCWGGT 1 cut(s) 1546
SfaNI GCATC 8 cut(s) 31, 241, 448, 775, 1491, 1698, 1729, 1925
SfcI CTRYAG 3 cut(s) 663, 717, 1632
Sfr274I CTCGAG 2 cut(s) 1101, 1817
SgrAI CRCCGGYG 1 cut(s) 65
SinI GGWCC 8 cut(s) 41, 328, 575, 701, 1417, 1466, 1792, 1864
SlaI CTCGAG 2 cut(s) 1101, 1817
SmiMI CAYNNNNRTG 1 cut(s) 1619
SmlI CTYRAG 3 cut(s) 986, 1101, 1817
SmoI CTYRAG 3 cut(s) 986, 1101, 1817
SnaBI TACGTA 1 cut(s) 571
SphI GCATGC 3 cut(s) 547, 1618, 1780
Sse8387I CCTGCAGG 1 cut(s) 1636
SseBI AGGCCT 2 cut(s) 1219, 1378
SspI AATATT 2 cut(s) 361, 996
SspMI CTAG 3 cut(s) 266, 918, 1283
SstI GAGCTC 1 cut(s) 1108
StuI AGGCCT 2 cut(s) 1219, 1378
StyD4I CCNGG 7 cut(s) 165, 1267, 1276, 1366, 1378, 1444, 1546
StyI CCWWGG 3 cut(s) 474, 1520, 1764
TaaI ACNGT 1 cut(s) 1015
TaiI ACGT 7 cut(s) 95, 105, 573, 642, 1459, 1531, 1645
TaqI TCGA 4 cut(s) 1082, 1102, 1453, 1818
TaqII GACCGA 1 cut(s) 143
TatI WGTACW 1 cut(s) 271
TfiI GAWTC 4 cut(s) 10, 373, 656, 1516
Tru1I TTAA 6 cut(s) 231, 536, 551, 987, 1893, 1948
Tru9I TTAA 6 cut(s) 231, 536, 551, 987, 1893, 1948
TscAI CASTG 4 cut(s) 294, 718, 1245, 1503
TseFI GTSAC 5 cut(s) 34, 124, 1015, 1037, 1821
TseI GCWGC 6 cut(s) 136, 254, 512, 788, 1486, 1760
Tsp45I GTSAC 5 cut(s) 34, 124, 1015, 1037, 1821
TspDTI ATGAA 3 cut(s) 923, 1133, 1659
TspGWI ACGGA 5 cut(s) 166, 470, 575, 1127, 1183
TspRI CASTG 4 cut(s) 294, 718, 1245, 1503
Van91I CCANNNNNTGG 2 cut(s) 293, 1577
Vha464I CTTAAG 1 cut(s) 986
VpaK11BI GGWCC 8 cut(s) 41, 328, 575, 701, 1417, 1466, 1792, 1864
XapI RAATTY 6 cut(s) 309, 623, 684, 893, 926, 940
XceI RCATGY 3 cut(s) 547, 1618, 1780
XhoI CTCGAG 2 cut(s) 1101, 1817
XmiI GTMKAC 2 cut(s) 636, 1453
XspI CTAG 3 cut(s) 266, 918, 1283
ZrmI AGTACT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.