MD06G1116100.v1.1

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000408
Physical Location & Seq
Forward (+)
25495425 .. 25498691
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1116100.v1.1.491

Sequence Viewer

Length: 2841 bp
ATGGCTAAGAGTGCAGTTGGATTGAATGCACTACGTGTGGAGGGAACAAGGGACATGACTGAGACATTGGATCCGGAAGCGAATATAATGGTAGGGTGGCAGAAAGTTGAAGAGAGTTGGGTGGCACAAAGTAAAACTGAACATCCATCACGTACAGTTGGACTGGATGCAATGAACATGCAGGTGGGAGCAAGGAGTATGGAGGAGACCTCTGTAAGTGATATAGAGGTGGGGCTAAAGCGAATTCAGGGTAAAGAAGTATCTGAACTTGATGCGCCATTTAGGGAATTGAAGGAGGATGTTCTTAAGGCTGAGGAAGATGAGGAACTTGATGAGCGGGTGGAAGATCATCATGACAGGAAGAAGAAGGGAGCTGAGTCTGTGATAGGTGCAAGTGGAGACGGGATGGCGATTTGTAAAGCTGATGGGTGTGCAGTCGATCTGAGTGATGCAAAGGTGTATAATAGAAAGCATAGGGTGTGCAAACTTCATTCCAAGGCTCCACTTGTGATTGTGTCTGGTCTAAAGAGAAGGTTTCGCCATAAATGCAGCAGATTTCATGATCTGTCAGAATTTGATGACACCAAACAAAGTTGTCGTAGGCATTTGCCCTACATTGAACGCGATGGTACAAGCACTCAGTTAAAGGATATAAGTGGTGATATAGATACGGTGATCAGTCAAATTGAATGGAGTGGTGTGGTACAATCACAAAATGATCAAAGTTCATATGAAGTGGTCGAAGCCACACCACCAGCAGTTGGATTGAATGTACAGAGTGTGAAGGGAACAAGGGATGCAAAGGAGACATTGCAGACCTCAGCAACAGCAAGTAATACAGAGGCAGGACTGGAGCAAATTCAGTGGAAAGACGTGCTTAATTTTGACCCTCCGTACGCTGAATATAAGGAAACTGAATACATACCTTCATTGGTTACAGTTGACTTGGATGTGATTTTGAGAGGATTGAAGAGGTTTCTTCTTTATGATGAGGTGTCAGCGGTTGTGCTAACGGGTCATCAAGGAAGTGGGAAAACCACATTGGCAAAATACTTCTGTGCAGATGAGGAAGTTAAAAACATATTCAAGAACAACATCTTCTTTGTTCTTGTTTCAAAAAATCCTAGCTGGAGCCTTATGGTACCTGCGTTGCAAGACGAATCAATTGCACTTCACTGGTTGCACCAATTTTTCATGGAAACAGCACTTCACCCATCAGTGTTGGTCTTGGATGATGTTTGGCCTGGATCGGAATCCCTTCTTGACAAGTTTGATGAATTCAAAAGACCAAACTACAAGATTATGGTGACATCAAGGTCTGAATTTCCAGCATTTGGTTGTCCATATTATCTGAAATCACTGAATGCTGAAGATGAGATGACTCTTTCTCATCAATCCCACACACACTTCGAATCACTGGATGATGAAGACCTGACAAGTGTTTCTCCGCCACCCTCCGTCACATTTGATGATAAAAGCTTGATAGATCATTTTCCTGATTCCTCTGCGTCCGACTTGGGAGACAGAAGCTCTCATGTTTCAACAGCTATCAGGCCAATGGATCAATTTCAGGATTCCTCCGCATCAACCTTGGGAGATAAGAGCTCGTGCGTTCCAAAAGATGCAGGGACAATGACATATATGCAGAAGCTGGTATTCAAGGTCAAGAAAGAAAAGAAGAAAGCCATGAAAGCTGTTTCTGGTGTTGAAGATCTTCTCGGGCGAAAAAAAAAGGAATCGGCCCTTGACGTTTATATCGACAGTGGTAACCAATCAGGAACTGCCAAAGGCTGGAAGCTAACAGGTGCCCGGGAAGCATTAAGCATCAACCTCGCGACATTTGAGAAGCCCCTTCGAAAGCTCACTTTTGCAGATCTTCTTGAGGCCACCAATGGTTTCCACAATAACAGCCTTATTGGCAAAGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAAGATGGCAGTGTCGTAGCCATAAAGAAACTAATACATATCAGCGGACATGGTGATCGCGAATTCACTGCAGAAATGGAAACAATAGGGAAGATCAAGCACCGGAACCTTGTCCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAATGGCTTTTTGTTTATGAGTACATGAAATATGGAAGCTTAGATGATGTTTTACATGAACAGAAGAAAGCTGGCGTCAAGTTGAACTGGGCTGCAAGGAGGAAGATTGCCATTGGGTCCGCGAGGGGACTGGCCTTTCTTCACCACAATTGCATCCCACACATCATTCACAGGGATATGAAATCGAGCAACGTGCTGGTGGATGAAAATTTGGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTGCAATGGACACCCATTTGAGTGTGAGCACTCTAGCAGGCACCCCTGGTTATGTCCCTCCTGAATACTACCAGAGCTTTAGATGTTCCACCAAAGGTGATGTTTATAGTTATGGAGTAGTATTGCTTGAGCTGCTAACGGGAAGACGTCCTACAGATTCAGCAGATTTTGGCGACAATAATCTCGTGGGTTGGGTAAAACAACACGCTAAATTGAAAATAAGTGATGTTTTTGATCCGGAGCTCATGAAAGAGGACGCAAGCCTTGAGATTGAGCTTTTACAGCACTTGAAGGTAGCTTGTGCTTGTTTGGACGACAGGCCATGGCGGCGTCCGACAATGATCCAAGTGATGGAAATGTTCAAGGAAATCCAAGCCGGGTCTGGGATGGCCTCCCAATCAACGATAGCCACGGACGACGACGGAGTTTTTGGCGGAGTTGAAATGGTAGAGATGTGCATAAAAGAAGTCCCGGAAAGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Pkinase zf-RVT

Protein Analysis

947

Amino Acids

105.39

Weight (kDa)

5.57

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 139 - 203 1.8e-21 SBP domain
NB-ARC PF00931 323 - 441 2.6e-07 NB-ARC domain
Pkinase PF00069 633 - 903 1.2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 636 - 903 3.6e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
AasI GACNNNNNNGTC 1 cut(s) 1315
AatII GACGTC 1 cut(s) 2503
Acc36I ACCTGC 2 cut(s) 172, 1153
Acc65I GGTACC 1 cut(s) 1141
AccB1I GGYRCC 3 cut(s) 1141, 1805, 2393
AccB7I CCANNNNNTGG 4 cut(s) 761, 1334, 1791, 2704
AccBSI CCGCTC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 1941
AccII CGCG 4 cut(s) 624, 1835, 2016, 2222
AccIII TCCGGA 2 cut(s) 73, 2590
AciI CCGC 8 cut(s) 337, 1001, 1448, 1581, 2001, 2220, 2680, 2787
AclWI GGATC 6 cut(s) 65, 78, 1255, 1569, 2582, 2689
AcsI RAATTY 7 cut(s) 243, 572, 858, 1277, 1322, 2018, 2308
AcuI CTGAAG 1 cut(s) 1389
AcyI GRCGYC 3 cut(s) 2175, 2500, 2683
AdeI CACNNNGTG 1 cut(s) 35
AfaI GTAC 7 cut(s) 154, 631, 705, 774, 896, 1143, 2123
AfiI CCNNNNNNNGG 6 cut(s) 761, 1334, 1791, 2078, 2704, 2736
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 874
AjnI CCWGG 2 cut(s) 1243, 2398
AjuI GAANNNNNNNTTGG 2 cut(s) 1025, 1057
Alw21I GWGCWC 3 cut(s) 1607, 2384, 2598
Alw26I GTCTC 6 cut(s) 56, 200, 393, 800, 1515, 2329
AlwI GGATC 6 cut(s) 65, 78, 1255, 1569, 2582, 2689
AlwNI CAGNNNCTG 2 cut(s) 1651, 1781
Ama87I CYCGRG 2 cut(s) 1718, 1809
Aor13HI TCCGGA 2 cut(s) 73, 2590
AoxI GGCC 8 cut(s) 1241, 1553, 1740, 1884, 1947, 2232, 2672, 2742
ApeKI GCWGC 3 cut(s) 549, 2192, 2485
ApoI RAATTY 7 cut(s) 243, 572, 858, 1277, 1322, 2018, 2308
ArsI GACNNNNNNTTYG 2 cut(s) 2765, 2797
Asp700I GAANNNNTTC 2 cut(s) 1257, 1713
Asp718I GGTACC 1 cut(s) 1141
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 3 cut(s) 1741, 1948, 2217
AsuC2I CCSGG 4 cut(s) 1810, 1811, 2731, 2825
AsuHPI GGTGA 8 cut(s) 671, 685, 1202, 1318, 1946, 2021, 2234, 2462
AsuII TTCGAA 2 cut(s) 1410, 1855
AvaI CYCGRG 2 cut(s) 1718, 1809
AvaII GGWCC 1 cut(s) 2217
BaeGI GKGCMC 1 cut(s) 1810
BaeI ACNNNNGTAYC 2 cut(s) 1133, 1166
BamHI GGATCC 1 cut(s) 70
BanI GGYRCC 3 cut(s) 1141, 1805, 2393
BanII GRGCYC 2 cut(s) 1607, 2598
BarI GAAGNNNNNNTAC 2 cut(s) 2488, 2520
BauI CACGAG 2 cut(s) 1606, 2537
BbsI GAAGAC 2 cut(s) 1434, 2503
Bbv12I GWGCWC 3 cut(s) 1607, 2384, 2598
BbvCI CCTCAGC 2 cut(s) 312, 820
BbvI GCAGC 3 cut(s) 561, 2179, 2472
BccI CCATC 8 cut(s) 154, 400, 419, 620, 1222, 1955, 2698, 2734
BcgI CGANNNNNNTGC 8 cut(s) 1148, 1182, 1813, 1847, 2006, 2040, 2275, 2309
BciT130I CCWGG 2 cut(s) 1245, 2400
BciVI GTATCC 1 cut(s) 2075
BclI TGATCA 2 cut(s) 675, 718
BcnI CCSGG 4 cut(s) 1810, 1811, 2731, 2825
BcoDI GTCTC 6 cut(s) 56, 200, 393, 800, 1515, 2329
BfaI CTAG 2 cut(s) 1125, 2387
BfmI CTRYAG 2 cut(s) 2025, 2505
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 2 cut(s) 172, 1153
BfuI GTATCC 1 cut(s) 2075
BglI GCCNNNNNGGC 2 cut(s) 1917, 2680
BglII AGATCT 2 cut(s) 1711, 1873
BisI GCNGC 4 cut(s) 550, 2193, 2486, 2681
BlsI GCNGC 4 cut(s) 551, 2194, 2487, 2682
Bme1390I CCNGG 6 cut(s) 1245, 1810, 1811, 2400, 2731, 2825
Bme18I GGWCC 1 cut(s) 2217
BmeT110I CYCGRG 2 cut(s) 1718, 1809
BmgBI CACGTC 1 cut(s) 874
BmgT120I GGNCC 3 cut(s) 1741, 1948, 2217
BmiI GGNNCC 8 cut(s) 72, 501, 1133, 1143, 1807, 2063, 2218, 2395
BmrFI CCNGG 6 cut(s) 1245, 1810, 1811, 2400, 2731, 2825
BmrI ACTGGG 1 cut(s) 2197
BmsI GCATC 8 cut(s) 157, 262, 439, 787, 1592, 1612, 1833, 2262
BmuI ACTGGG 1 cut(s) 2197
BpiI GAAGAC 2 cut(s) 1434, 2503
BplI GAGNNNNNCTC 2 cut(s) 194, 226
BpmI CTGGAG 2 cut(s) 872, 1150
Bpu10I CCTNAGC 2 cut(s) 312, 820
Bpu14I TTCGAA 2 cut(s) 1410, 1855
BpuEI CTTGAG 3 cut(s) 1901, 2501, 2639
BpuMI CCSGG 4 cut(s) 1810, 1811, 2731, 2825
BsaAI YACGTR 2 cut(s) 35, 152
BsaBI GATNNNNATC 1 cut(s) 1252
BsaHI GRCGYC 3 cut(s) 2175, 2500, 2683
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 7 cut(s) 495, 1590, 1809, 2077, 2398, 2675, 2763
BsaWI WCCGGW 3 cut(s) 73, 2058, 2590
Bsc4I CCNNNNNNNGG 6 cut(s) 761, 1334, 1791, 2078, 2704, 2736
Bse1I ACTGG 6 cut(s) 168, 855, 1181, 1422, 2192, 2235
Bse3DI GCAATG 3 cut(s) 177, 809, 2364
Bse8I GATNNNNATC 1 cut(s) 1252
BseAI TCCGGA 2 cut(s) 73, 2590
BseBI CCWGG 2 cut(s) 1245, 2400
BseDI CCNNGG 7 cut(s) 495, 1590, 1809, 2077, 2398, 2675, 2763
BseJI GATNNNNATC 1 cut(s) 1252
BseLI CCNNNNNNNGG 6 cut(s) 761, 1334, 1791, 2078, 2704, 2736
BseMI GCAATG 3 cut(s) 177, 809, 2364
BseMII CTCAG 6 cut(s) 51, 303, 366, 434, 653, 834
BseNI ACTGG 6 cut(s) 168, 855, 1181, 1422, 2192, 2235
BseRI GAGGAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 1810
BseXI GCAGC 3 cut(s) 561, 2179, 2472
BsgI GTGCAG 3 cut(s) 33, 453, 1080
Bsh1236I CGCG 4 cut(s) 624, 1835, 2016, 2222
BshFI GGCC 8 cut(s) 1243, 1555, 1742, 1886, 1949, 2234, 2674, 2744
BshNI GGYRCC 3 cut(s) 1141, 1805, 2393
BsiHKAI GWGCWC 3 cut(s) 1607, 2384, 2598
BsiHKCI CYCGRG 2 cut(s) 1718, 1809
BsiSI CCGG 6 cut(s) 74, 1810, 2059, 2591, 2730, 2825
BsiWI CGTACG 1 cut(s) 894
BslFI GGGAC 6 cut(s) 65, 1642, 2054, 2241, 2393, 2807
BslI CCNNNNNNNGG 6 cut(s) 761, 1334, 1791, 2078, 2704, 2736
BsmAI GTCTC 6 cut(s) 56, 200, 393, 800, 1515, 2329
BsmBI CGTCTC 1 cut(s) 393
BsmFI GGGAC 6 cut(s) 65, 1642, 2054, 2241, 2393, 2807
BsmI GAATGC 2 cut(s) 31, 1369
BsnI GGCC 8 cut(s) 1243, 1555, 1742, 1886, 1949, 2234, 2674, 2744
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 2 cut(s) 1718, 1809
Bsp119I TTCGAA 2 cut(s) 1410, 1855
Bsp1286I GDGCHC 4 cut(s) 1607, 1810, 2384, 2598
Bsp13I TCCGGA 2 cut(s) 73, 2590
Bsp1407I TGTACA 1 cut(s) 772
Bsp19I CCATGG 1 cut(s) 2675
Bsp68I TCGCGA 2 cut(s) 1835, 2016
BspACI CCGC 8 cut(s) 337, 1001, 1448, 1581, 2001, 2220, 2680, 2787
BspANI GGCC 8 cut(s) 1243, 1555, 1742, 1886, 1949, 2234, 2674, 2744
BspCNI CTCAG 6 cut(s) 52, 304, 367, 435, 652, 833
BspEI TCCGGA 2 cut(s) 73, 2590
BspFNI CGCG 4 cut(s) 624, 1835, 2016, 2222
BspHI TCATGA 3 cut(s) 352, 559, 2598
BspLI GGNNCC 8 cut(s) 72, 501, 1133, 1143, 1807, 2063, 2218, 2395
BspMAI CTGCAG 1 cut(s) 2029
BspMI ACCTGC 2 cut(s) 172, 1153
BspPI GGATC 6 cut(s) 65, 78, 1255, 1569, 2582, 2689
BspT104I TTCGAA 2 cut(s) 1410, 1855
BspT107I GGYRCC 3 cut(s) 1141, 1805, 2393
BspTI CTTAAG 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 337
BsrDI GCAATG 3 cut(s) 177, 809, 2364
BsrGI TGTACA 1 cut(s) 772
BsrI ACTGG 6 cut(s) 168, 855, 1181, 1422, 2192, 2235
BssECI CCNNGG 7 cut(s) 495, 1590, 1809, 2077, 2398, 2675, 2763
BssNAI GTATAC 1 cut(s) 1942
BssNI GRCGYC 3 cut(s) 2175, 2500, 2683
BssSI CACGAG 2 cut(s) 1606, 2537
BssT1I CCWWGG 4 cut(s) 495, 1590, 2077, 2675
Bst1107I GTATAC 1 cut(s) 1942
Bst2BI CACGAG 2 cut(s) 1606, 2537
Bst2UI CCWGG 2 cut(s) 1245, 2400
Bst4CI ACNGT 4 cut(s) 157, 673, 940, 1763
Bst6I CTCTTC 2 cut(s) 105, 965
BstACI GRCGYC 3 cut(s) 2175, 2500, 2683
BstAFI CTTAAG 1 cut(s) 305
BstAUI TGTACA 1 cut(s) 772
BstBAI YACGTR 2 cut(s) 35, 152
BstBI TTCGAA 2 cut(s) 1410, 1855
BstC8I GCNNGC 4 cut(s) 2173, 2392, 2614, 2834
BstDEI CTNAG 8 cut(s) 6, 60, 312, 375, 443, 639, 820, 2140
BstDSI CCRYGG 2 cut(s) 2675, 2763
BstEII GGTNACC 1 cut(s) 1766
BstFNI CGCG 4 cut(s) 624, 1835, 2016, 2222
BstHHI GCGC 1 cut(s) 277
BstMAI GTCTC 6 cut(s) 56, 200, 393, 800, 1515, 2329
BstMWI GCNNNNNNNGC 8 cut(s) 11, 546, 1691, 1814, 1917, 2485, 2635, 2680
BstNI CCWGG 2 cut(s) 1245, 2400
BstNSI RCATGY 1 cut(s) 181
BstPI GGTNACC 1 cut(s) 1766
BstSCI CCNGG 6 cut(s) 1243, 1808, 1809, 2398, 2729, 2823
BstSFI CTRYAG 2 cut(s) 2025, 2505
BstSLI GKGCMC 1 cut(s) 1810
BstUI CGCG 4 cut(s) 624, 1835, 2016, 2222
BstV1I GCAGC 3 cut(s) 561, 2179, 2472
BstV2I GAAGAC 2 cut(s) 1434, 2503
BstX2I RGATCY 3 cut(s) 70, 1711, 1873
BstYI RGATCY 3 cut(s) 70, 1711, 1873
BstZ17I GTATAC 1 cut(s) 1942
BsuI GTATCC 1 cut(s) 2075
BsuRI GGCC 8 cut(s) 1243, 1555, 1742, 1886, 1949, 2234, 2674, 2744
BtgI CCRYGG 2 cut(s) 2675, 2763
BtgZI GCGATG 1 cut(s) 639
BtrI CACGTC 1 cut(s) 874
BtsI GCAGTG 2 cut(s) 1972, 2022
BtsIMutI CAGTG 8 cut(s) 869, 1174, 1224, 1358, 1415, 1768, 1972, 2022
BtuMI TCGCGA 2 cut(s) 1835, 2016
BveI ACCTGC 2 cut(s) 172, 1153
Cac8I GCNNGC 4 cut(s) 2173, 2392, 2614, 2834
CaiI CAGNNNCTG 2 cut(s) 1651, 1781
CciI TCATGA 3 cut(s) 352, 559, 2598
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 3 cut(s) 1741, 1948, 2217
Cfr9I CCCGGG 1 cut(s) 1809
CseI GACGC 4 cut(s) 1497, 2164, 2618, 2672
Csp6I GTAC 7 cut(s) 153, 630, 704, 773, 895, 1142, 2122
CspCI CAANNNNNGTGG 2 cut(s) 845, 880
CviQI GTAC 7 cut(s) 153, 630, 704, 773, 895, 1142, 2122
DdeI CTNAG 8 cut(s) 6, 60, 312, 375, 443, 639, 820, 2140
DraIII CACNNNGTG 1 cut(s) 35
DrdI GACNNNNNNGTC 1 cut(s) 1315
DseDI GACNNNNNNGTC 1 cut(s) 1315
Eam1104I CTCTTC 2 cut(s) 105, 965
EarI CTCTTC 2 cut(s) 105, 965
EciI GGCGGA 2 cut(s) 1437, 2802
Ecl136II GAGCTC 2 cut(s) 1605, 2596
Eco130I CCWWGG 4 cut(s) 495, 1590, 2077, 2675
Eco24I GRGCYC 2 cut(s) 1607, 2598
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 2217
Eco53kI GAGCTC 2 cut(s) 1605, 2596
Eco57I CTGAAG 1 cut(s) 1389
Eco88I CYCGRG 2 cut(s) 1718, 1809
Eco91I GGTNACC 1 cut(s) 1766
EcoICRI GAGCTC 2 cut(s) 1605, 2596
EcoO65I GGTNACC 1 cut(s) 1766
EcoRI GAATTC 3 cut(s) 243, 1277, 2018
EcoRII CCWGG 2 cut(s) 1243, 2398
EcoT14I CCWWGG 4 cut(s) 495, 1590, 2077, 2675
EcoT38I GRGCYC 2 cut(s) 1607, 2598
ErhI CCWWGG 4 cut(s) 495, 1590, 2077, 2675
Esp3I CGTCTC 1 cut(s) 393
FalI AAGNNNNNCTT 4 cut(s) 861, 893, 2091, 2123
FaqI GGGAC 6 cut(s) 65, 1642, 2054, 2241, 2393, 2807
FauI CCCGC 1 cut(s) 330
FauNDI CATATG 1 cut(s) 730
FbaI TGATCA 2 cut(s) 675, 718
FblI GTMKAC 1 cut(s) 1941
Fnu4HI GCNGC 4 cut(s) 550, 2193, 2486, 2681
FriOI GRGCYC 2 cut(s) 1607, 2598
Fsp4HI GCNGC 4 cut(s) 550, 2193, 2486, 2681
FspBI CTAG 2 cut(s) 1125, 2387
GlaI GCGC 1 cut(s) 276
GluI GCNGC 4 cut(s) 550, 2193, 2486, 2681
GsuI CTGGAG 2 cut(s) 872, 1150
HaeIII GGCC 8 cut(s) 1243, 1555, 1742, 1886, 1949, 2234, 2674, 2744
HapII CCGG 6 cut(s) 74, 1810, 2059, 2591, 2730, 2825
HgaI GACGC 4 cut(s) 1497, 2164, 2618, 2672
HhaI GCGC 1 cut(s) 277
Hin1I GRCGYC 3 cut(s) 2175, 2500, 2683
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HindIII AAGCTT 2 cut(s) 1477, 2137
HpaII CCGG 6 cut(s) 74, 1810, 2059, 2591, 2730, 2825
HphI GGTGA 8 cut(s) 671, 685, 1202, 1318, 1946, 2021, 2234, 2462
Hpy166II GTNNAC 2 cut(s) 943, 1942
Hpy188I TCNGA 9 cut(s) 265, 444, 571, 1252, 1321, 1353, 1513, 2329, 2688
Hpy8I GTNNAC 2 cut(s) 943, 1942
Hpy99I CGWCG 2 cut(s) 2774, 2777
HpyAV CCTTC 8 cut(s) 286, 361, 525, 778, 936, 1268, 1862, 2638
HpyCH4III ACNGT 4 cut(s) 157, 673, 940, 1763
HpyCH4IV ACGT 6 cut(s) 34, 151, 873, 1749, 2292, 2500
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 546, 1691, 1814, 1917, 2485, 2635, 2680
HpyF3I CTNAG 8 cut(s) 6, 60, 312, 375, 443, 639, 820, 2140
HpySE526I ACGT 6 cut(s) 34, 151, 873, 1749, 2292, 2500
Hsp92I GRCGYC 3 cut(s) 2175, 2500, 2683
HspAI GCGC 1 cut(s) 275
Kpn2I TCCGGA 2 cut(s) 73, 2590
KpnI GGTACC 1 cut(s) 1145
Ksp22I TGATCA 2 cut(s) 675, 718
LmnI GCTCC 6 cut(s) 188, 371, 505, 853, 1131, 2593
Lsp1109I GCAGC 3 cut(s) 561, 2179, 2472
LweI GCATC 8 cut(s) 157, 262, 439, 787, 1592, 1612, 1833, 2262
MaeI CTAG 2 cut(s) 1125, 2387
MaeII ACGT 6 cut(s) 34, 151, 873, 1749, 2292, 2500
MaeIII GTNAC 4 cut(s) 934, 1306, 1459, 1766
MbiI CCGCTC 1 cut(s) 337
MfeI CAATTG 3 cut(s) 1164, 1952, 2248
MflI RGATCY 3 cut(s) 70, 1711, 1873
MhlI GDGCHC 4 cut(s) 1607, 1810, 2384, 2598
MlyI GAGTC 3 cut(s) 386, 1375, 2331
MmeI TCCRAC 4 cut(s) 139, 742, 1536, 2711
MroI TCCGGA 2 cut(s) 73, 2590
MroXI GAANNNNTTC 2 cut(s) 1257, 1713
MseI TTAA 5 cut(s) 306, 644, 879, 1074, 1820
MslI CAYNNNNRTG 2 cut(s) 182, 2373
MspA1I CMGCKG 2 cut(s) 1001, 2001
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 6 cut(s) 74, 1810, 2059, 2591, 2730, 2825
MspR9I CCNGG 6 cut(s) 1245, 1810, 1811, 2400, 2731, 2825
MunI CAATTG 3 cut(s) 1164, 1952, 2248
Mva1269I GAATGC 2 cut(s) 31, 1369
MvaI CCWGG 2 cut(s) 1245, 2400
MvnI CGCG 4 cut(s) 624, 1835, 2016, 2222
MwoI GCNNNNNNNGC 8 cut(s) 11, 546, 1691, 1814, 1917, 2485, 2635, 2680
NciI CCSGG 4 cut(s) 1810, 1811, 2731, 2825
NcoI CCATGG 1 cut(s) 2675
NdeI CATATG 1 cut(s) 730
NlaIV GGNNCC 8 cut(s) 72, 501, 1133, 1143, 1807, 2063, 2218, 2395
NmuCI GTSAC 2 cut(s) 1306, 1459
NruI TCGCGA 2 cut(s) 1835, 2016
NspI RCATGY 1 cut(s) 181
NspV TTCGAA 2 cut(s) 1410, 1855
PagI TCATGA 3 cut(s) 352, 559, 2598
PaqCI CACCTGC 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 1755
PctI GAATGC 2 cut(s) 31, 1369
PdmI GAANNNNTTC 2 cut(s) 1257, 1713
PfeI GAWTC 7 cut(s) 1160, 1253, 1412, 1499, 1574, 1736, 2510
Pfl23II CGTACG 1 cut(s) 894
PflMI CCANNNNNTGG 4 cut(s) 761, 1334, 1791, 2704
PfoI TCCNGGA 1 cut(s) 2823
PkrI GCNGC 4 cut(s) 551, 2194, 2487, 2682
PleI GAGTC 3 cut(s) 385, 1375, 2330
PpsI GAGTC 3 cut(s) 385, 1375, 2330
Ppu21I YACGTR 2 cut(s) 35, 152
Psp124BI GAGCTC 2 cut(s) 1607, 2598
Psp6I CCWGG 2 cut(s) 1243, 2398
PspEI GGTNACC 1 cut(s) 1766
PspGI CCWGG 2 cut(s) 1243, 2398
PspLI CGTACG 1 cut(s) 894
PspN4I GGNNCC 8 cut(s) 72, 501, 1133, 1143, 1807, 2063, 2218, 2395
PspPI GGNCC 3 cut(s) 1741, 1948, 2217
PstI CTGCAG 1 cut(s) 2029
PstNI CAGNNNCTG 2 cut(s) 1651, 1781
PsuI RGATCY 3 cut(s) 70, 1711, 1873
RruI TCGCGA 2 cut(s) 1835, 2016
RsaI GTAC 7 cut(s) 154, 631, 705, 774, 896, 1143, 2123
RsaNI GTAC 7 cut(s) 153, 630, 704, 773, 895, 1142, 2122
RseI CAYNNNNRTG 2 cut(s) 182, 2373
SacI GAGCTC 2 cut(s) 1607, 2598
SaqAI TTAA 5 cut(s) 306, 644, 879, 1074, 1820
SatI GCNGC 4 cut(s) 550, 2193, 2486, 2681
Sau96I GGNCC 3 cut(s) 1741, 1948, 2217
SchI GAGTC 3 cut(s) 386, 1375, 2331
ScrFI CCNGG 6 cut(s) 1245, 1810, 1811, 2400, 2731, 2825
SduI GDGCHC 4 cut(s) 1607, 1810, 2384, 2598
SfaNI GCATC 8 cut(s) 157, 262, 439, 787, 1592, 1612, 1833, 2262
SfcI CTRYAG 2 cut(s) 2025, 2505
SfuI TTCGAA 2 cut(s) 1410, 1855
SinI GGWCC 1 cut(s) 2217
SmaI CCCGGG 1 cut(s) 1811
SmiMI CAYNNNNRTG 2 cut(s) 182, 2373
SmlI CTYRAG 4 cut(s) 305, 1880, 2480, 2618
SmoI CTYRAG 4 cut(s) 305, 1880, 2480, 2618
SsiI CCGC 8 cut(s) 337, 1001, 1448, 1581, 2001, 2220, 2680, 2787
SspMI CTAG 2 cut(s) 1125, 2387
SstI GAGCTC 2 cut(s) 1607, 2598
StyD4I CCNGG 6 cut(s) 1243, 1808, 1809, 2398, 2729, 2823
StyI CCWWGG 4 cut(s) 495, 1590, 2077, 2675
TaaI ACNGT 4 cut(s) 157, 673, 940, 1763
TaiI ACGT 6 cut(s) 37, 154, 876, 1752, 2295, 2503
TaqI TCGA 6 cut(s) 438, 741, 1410, 1758, 1855, 2285
TatI WGTACW 2 cut(s) 772, 2121
TauI GCSGC 1 cut(s) 2683
TfiI GAWTC 7 cut(s) 1160, 1253, 1412, 1499, 1574, 1736, 2510
Tru1I TTAA 5 cut(s) 306, 644, 879, 1074, 1820
Tru9I TTAA 5 cut(s) 306, 644, 879, 1074, 1820
TscAI CASTG 8 cut(s) 869, 1181, 1224, 1365, 1422, 1768, 1972, 2029
TseFI GTSAC 2 cut(s) 1306, 1459
TseI GCWGC 3 cut(s) 549, 2192, 2485
Tsp45I GTSAC 2 cut(s) 1306, 1459
TspGWI ACGGA 4 cut(s) 882, 1447, 2780, 2790
TspMI CCCGGG 1 cut(s) 1809
TspRI CASTG 8 cut(s) 869, 1181, 1224, 1365, 1422, 1768, 1972, 2029
Van91I CCANNNNNTGG 4 cut(s) 761, 1334, 1791, 2704
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 2217
XapI RAATTY 7 cut(s) 243, 572, 858, 1277, 1322, 2018, 2308
XceI RCATGY 1 cut(s) 181
XcmI CCANNNNNNNNNTGG 2 cut(s) 1958, 2732
XmaI CCCGGG 1 cut(s) 1809
XmiI GTMKAC 1 cut(s) 1941
XmnI GAANNNNTTC 2 cut(s) 1257, 1713
XspI CTAG 2 cut(s) 1125, 2387
ZraI GACGTC 1 cut(s) 2501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.