Rh7CG082600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000408
Physical Location & Seq
Forward (+)
6005868 .. 6007784
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG082600.1

Sequence Viewer

Length: 1917 bp
ATGTCTATGAGTCTGAACTTTCTGGGAGGAGCACCTTTTCAACTCCTATACGATGGTGTTAGGGTAGCCATAGGTAGAACTACCAAGTTCAAAGCCCTCCTCTTAAATCTCGAATCGACGCTAGACTGTTTGCAACCACGAGTCATCCAGCAGATAAGAGACCATAATGTGGAGCTGAATCTCCCAAACGATATAATTCAAGACCTGCAAGTGAAAATGGATGATGGCGTGGCACTCATTGCCAATTTATCTAGGCTTAGCGTGTGGAACTGTCGCGTATGGGGGGACTGTTGCAACTGCATGAAGCCTAGCTATGCAGACCAACTTGCTGCATTGGATAGGTCTCTTAGACGACTGTTGGAGATACTAAAGCTTGAGCAGATGAGGAATGTGGTGGAGCTCTTGCTTTTGGCAAGGAGAAGTAATGACCGACAAGATGACTTGGAGAGAAGACAGTTGGAAATTCTGAAAGCGCTGCAAGAAACCGGGGATATGTTAAGAGCGCAGCAGGAAGTAATGGAGAGAATTGAAAGGAATGGTGCTGCATCTTCAGACAGAGGAGGGTCAGCTTCTGCTCCTGCTCTAGGAACAGTATTTAGGGTGTTGTTTGATGTTGTTATCAGAGTCAAGGTCAAGAATATGATGTATGAGCGCCTCCTCAAAGATTTTGAATCCACTCTTGAATGCTTAAGGCCACTTATAGAAGAGATGGTGGAGTCTAACAAGTTGCTGCATCTTCCAAATGAGGAACAAGAATATTTCATAATGCAAATGGAAAAGGGTGTAGAGTTAATTCACCAGTGCTCTGAGGCTTGCAAGAGAGGAATAAGGTATAAAAAGTACGAATACACCAAGAAACTTCTGGGATTGGACGAATGTCTTCAAAGGCTTTTCATTGAACTCAAAGAGCAGGTAGTAAGGAATGTGAAGGAGGCCTTGGTGCTTATAAGTAACTTGGAAAAAGAGATTGCAGAAATTGAAGGGGTTATTGTGGTACAAAGTGATGAAATTGAGTGTTCGATTCCAGCACCTCAACCTCGATCACCTTTAGTTGGAGAGGATTTACAAAATGTGCAGATAAATCGAGAGGTGATCGAGGAGACATTCGATGCAGTAAGTAACTTAGAAGAGGAGATCAAGGAAATTAGAGAAGTTAATGTGGTACAAAATGATGAAGTACAAAGTAAAGGTCCTCAACCTTCATTGCCTATAGCTGGATCCAGTGTACAAAGTATGCAGGGAACAAGGGAAGTGATCAAGGAAAGATGGGACTCGGAAAAGTTGCCGGATGTCAAGCAAATTAAAGGGAAAGGCTTGTTCGAATATCCGGTTGCTGGTGAATATGACCTTCCACCATTTCCAGTGGGACTTGCACTGGAACGAGAAGATCACGCTGATGCATTCAGTAATGCATCATTGGAAGAACCGGTTGATGAATTGGATATGCATGTCAGCTACTTGAAACGGAAGCTTTTACATGGTGGTCACTTAGCCATTGTGCTGACGGGACCTGAAGGATGCGGGAAAACTAGATTGGCAGAAAAGTTTTGTCAAGATGAGGAAGTCATAGATGAATTCAAGAACAATATTTTCTTCGTCCCTGTTTCGGAAAGCCCCAAATTGCAGCTTATTGTTCAAAAACTATATCCAGAGGAGGGCTACAGGAAAATTATGTTGCCAGATGAAAATGGAGTTCATGCAATTCAGTGGCTGCAAGCATTTGTTAAGCATGTAGGACATCATCGTTCTCTGTTGGTCCTAGATGATGTCTGGCCTGGATCTGAATCCCTTCTTGATGAGTTTGATGAATTCAAAAGGTCAAATTTCAAGATTCTGGTGACATCAAGATTCGAATTTCCAAGATTTGGTTCCTCCTATTATGTAATGACGGATGATGTACTTAGGGTAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

638

Amino Acids

72.95

Weight (kDa)

5.07

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 137 5e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 193 - 323 2.7e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 483 - 616 1.2e-10 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 947
Acc36I ACCTGC 2 cut(s) 213, 901
AccB7I CCANNNNNTGG 2 cut(s) 169, 1865
AccII CGCG 1 cut(s) 276
AciI CCGC 1 cut(s) 1521
AclWI GGATC 3 cut(s) 1212, 1225, 1786
AcsI RAATTY 5 cut(s) 462, 1574, 1808, 1822, 1853
AcuI CTGAAG 2 cut(s) 534, 1533
AfaI GTAC 6 cut(s) 842, 996, 1164, 1179, 1227, 1899
AfeI AGCGCT 1 cut(s) 474
AfiI CCNNNNNNNGG 8 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1655, 1865
AflII CTTAAG 1 cut(s) 688
AgeI ACCGGT 1 cut(s) 1426
AjnI CCWGG 1 cut(s) 1774
AjuI GAANNNNNNNTTGG 6 cut(s) 920, 952, 1517, 1549, 1852, 1884
AluBI AGCT 9 cut(s) 175, 312, 373, 400, 569, 1214, 1455, 1471, 1627
AluI AGCT 9 cut(s) 175, 312, 373, 400, 569, 1214, 1455, 1471, 1627
Alw21I GWGCWC 3 cut(s) 34, 402, 806
Alw26I GTCTC 3 cut(s) 153, 348, 1094
AlwI GGATC 3 cut(s) 1212, 1225, 1786
AlwNI CAGNNNCTG 2 cut(s) 572, 1711
Aor51HI AGCGCT 1 cut(s) 474
AoxI GGCC 3 cut(s) 692, 933, 1772
ApeKI GCWGC 7 cut(s) 329, 475, 505, 542, 730, 1624, 1711
ApoI RAATTY 5 cut(s) 462, 1574, 1808, 1822, 1853
ArsI GACNNNNNNTTYG 2 cut(s) 1174, 1206
AsiGI ACCGGT 1 cut(s) 1426
Asp700I GAANNNNTTC 2 cut(s) 879, 1788
AspLEI GCGC 3 cut(s) 475, 505, 654
AspS9I GGNCC 3 cut(s) 1190, 1508, 1756
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 5 cut(s) 788, 1035, 1102, 1349, 1849
AsuII TTCGAA 2 cut(s) 1320, 1851
AvaII GGWCC 3 cut(s) 1190, 1508, 1756
BamHI GGATCC 1 cut(s) 1217
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 457, 872
Bbv12I GWGCWC 3 cut(s) 34, 402, 806
BbvI GCAGC 7 cut(s) 316, 462, 517, 529, 717, 1636, 1698
BccI CCATC 4 cut(s) 47, 218, 703, 1260
BcgI CGANNNNNNTGC 2 cut(s) 1064, 1098
BciT130I CCWGG 1 cut(s) 1776
BclI TGATCA 1 cut(s) 1254
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 3 cut(s) 153, 348, 1094
BfaI CTAG 6 cut(s) 122, 252, 309, 584, 1530, 1760
BfmI CTRYAG 2 cut(s) 1209, 1660
BfoI RGCGCY 2 cut(s) 476, 655
BfrI CTTAAG 1 cut(s) 688
BfuAI ACCTGC 2 cut(s) 213, 901
BisI GCNGC 7 cut(s) 330, 476, 506, 543, 731, 1625, 1712
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 7 cut(s) 331, 477, 507, 544, 732, 1626, 1713
Bme1390I CCNGG 2 cut(s) 487, 1776
Bme18I GGWCC 3 cut(s) 1190, 1508, 1756
BmgT120I GGNCC 3 cut(s) 1190, 1508, 1756
BmiI GGNNCC 3 cut(s) 1219, 1509, 1870
BmrFI CCNGG 2 cut(s) 487, 1776
BmsI GCATC 6 cut(s) 554, 742, 1099, 1387, 1421, 1508
BoxI GACNNNNGTC 1 cut(s) 876
BpiI GAAGAC 2 cut(s) 457, 872
Bpu1102I GCTNAGC 1 cut(s) 257
Bpu14I TTCGAA 2 cut(s) 1320, 1851
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 1783
BsaI GGTCTC 2 cut(s) 153, 348
BsaJI CCNNGG 2 cut(s) 486, 936
BsaWI WCCGGW 2 cut(s) 1327, 1426
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1655, 1865
Bse118I RCCGGY 1 cut(s) 1426
Bse1I ACTGG 4 cut(s) 799, 1221, 1361, 1380
Bse3DI GCAATG 2 cut(s) 237, 1202
Bse8I GATNNNNATC 1 cut(s) 1783
BseBI CCWGG 1 cut(s) 1776
BseDI CCNNGG 2 cut(s) 486, 936
BseGI GGATG 5 cut(s) 144, 226, 1294, 1523, 1897
BseJI GATNNNNATC 1 cut(s) 1783
BseLI CCNNNNNNNGG 8 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1655, 1865
BseMI GCAATG 2 cut(s) 237, 1202
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 4 cut(s) 799, 1221, 1361, 1380
BseRI GAGGAG 7 cut(s) 42, 89, 573, 647, 1112, 1145, 1667
BseXI GCAGC 7 cut(s) 316, 462, 517, 529, 717, 1636, 1698
BsgI GTGCAG 1 cut(s) 1094
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 3 cut(s) 694, 935, 1774
BshTI ACCGGT 1 cut(s) 1426
BsiHKAI GWGCWC 3 cut(s) 34, 402, 806
BsiSI CCGG 4 cut(s) 486, 1286, 1328, 1427
BslFI GGGAC 5 cut(s) 299, 1283, 1380, 1521, 1583
BslI CCNNNNNNNGG 8 cut(s) 169, 584, 1052, 1214, 1334, 1606, 1655, 1865
BsmAI GTCTC 3 cut(s) 153, 348, 1094
BsmFI GGGAC 5 cut(s) 299, 1283, 1380, 1521, 1583
BsmI GAATGC 2 cut(s) 689, 1400
BsnI GGCC 3 cut(s) 694, 935, 1774
Bso31I GGTCTC 2 cut(s) 153, 348
Bsp119I TTCGAA 2 cut(s) 1320, 1851
Bsp1286I GDGCHC 3 cut(s) 34, 402, 806
Bsp1407I TGTACA 1 cut(s) 1225
Bsp143I GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 1 cut(s) 1521
BspANI GGCC 3 cut(s) 694, 935, 1774
BspCNI CTCAG 1 cut(s) 799
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 3 cut(s) 1219, 1509, 1870
BspMI ACCTGC 2 cut(s) 213, 901
BspPI GGATC 3 cut(s) 1212, 1225, 1786
BspT104I TTCGAA 2 cut(s) 1320, 1851
BspTI CTTAAG 1 cut(s) 688
BspTNI GGTCTC 2 cut(s) 153, 348
BsrDI GCAATG 2 cut(s) 237, 1202
BsrFI RCCGGY 1 cut(s) 1426
BsrGI TGTACA 1 cut(s) 1225
BsrI ACTGG 4 cut(s) 799, 1221, 1361, 1380
BssAI RCCGGY 1 cut(s) 1426
BssECI CCNNGG 2 cut(s) 486, 936
BssMI GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 936
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 1776
Bst4CI ACNGT 6 cut(s) 128, 272, 290, 357, 456, 592
Bst6I CTCTTC 2 cut(s) 699, 1122
BstAFI CTTAAG 1 cut(s) 688
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 1225
BstBI TTCGAA 2 cut(s) 1320, 1851
BstC8I GCNNGC 2 cut(s) 814, 1716
BstDEI CTNAG 6 cut(s) 257, 347, 807, 1123, 1489, 1901
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 5 cut(s) 144, 226, 1294, 1523, 1897
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 2 cut(s) 476, 655
BstHHI GCGC 3 cut(s) 475, 505, 654
BstKTI GATC 7 cut(s) 1043, 1095, 1137, 1220, 1257, 1390, 1781
BstMAI GTCTC 3 cut(s) 153, 348, 1094
BstMBI GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 1776
BstNSI RCATGY 2 cut(s) 1451, 1733
BstPAI GACNNNNGTC 1 cut(s) 876
BstSCI CCNGG 2 cut(s) 485, 1774
BstSFI CTRYAG 2 cut(s) 1209, 1660
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 7 cut(s) 316, 462, 517, 529, 717, 1636, 1698
BstV2I GAAGAC 2 cut(s) 457, 872
BstX2I RGATCY 2 cut(s) 1217, 1778
BstYI RGATCY 2 cut(s) 1217, 1778
BsuRI GGCC 3 cut(s) 694, 935, 1774
BtsCI GGATG 5 cut(s) 144, 226, 1294, 1523, 1897
BtsIMutI CAGTG 5 cut(s) 806, 1228, 1368, 1373, 1712
BveI ACCTGC 2 cut(s) 213, 901
Cac8I GCNNGC 2 cut(s) 814, 1716
CaiI CAGNNNCTG 2 cut(s) 572, 1711
CfoI GCGC 3 cut(s) 475, 505, 654
Cfr10I RCCGGY 1 cut(s) 1426
Cfr13I GGNCC 3 cut(s) 1190, 1508, 1756
CseI GACGC 1 cut(s) 127
Csp6I GTAC 6 cut(s) 841, 995, 1163, 1178, 1226, 1898
CspAI ACCGGT 1 cut(s) 1426
CviAII CATG 5 cut(s) 301, 1448, 1478, 1697, 1730
CviQI GTAC 6 cut(s) 841, 995, 1163, 1178, 1226, 1898
DdeI CTNAG 6 cut(s) 257, 347, 807, 1123, 1489, 1901
DpnI GATC 7 cut(s) 1042, 1094, 1136, 1219, 1256, 1389, 1780
DpnII GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
Eam1104I CTCTTC 2 cut(s) 699, 1122
EarI CTCTTC 2 cut(s) 699, 1122
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 936
Eco147I AGGCCT 1 cut(s) 935
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 2 cut(s) 153, 348
Eco47I GGWCC 3 cut(s) 1190, 1508, 1756
Eco47III AGCGCT 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 534, 1533
EcoICRI GAGCTC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 2 cut(s) 1190, 1508
EcoRI GAATTC 2 cut(s) 1574, 1808
EcoRII CCWGG 1 cut(s) 1774
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 3 cut(s) 1402, 1414, 1449
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 5 cut(s) 304, 1451, 1481, 1700, 1733
FalI AAGNNNNNCTT 2 cut(s) 920, 952
FaqI GGGAC 5 cut(s) 299, 1283, 1380, 1521, 1583
FatI CATG 5 cut(s) 300, 1447, 1477, 1696, 1729
FauI CCCGC 1 cut(s) 1514
FbaI TGATCA 1 cut(s) 1254
Fnu4HI GCNGC 7 cut(s) 330, 476, 506, 543, 731, 1625, 1712
FokI GGATG 5 cut(s) 131, 233, 1301, 1530, 1904
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 7 cut(s) 330, 476, 506, 543, 731, 1625, 1712
FspBI CTAG 6 cut(s) 122, 252, 309, 584, 1530, 1760
GlaI GCGC 3 cut(s) 474, 504, 653
GluI GCNGC 7 cut(s) 330, 476, 506, 543, 731, 1625, 1712
HaeII RGCGCY 2 cut(s) 476, 655
HaeIII GGCC 3 cut(s) 694, 935, 1774
HapII CCGG 4 cut(s) 486, 1286, 1328, 1427
HgaI GACGC 1 cut(s) 127
HhaI GCGC 3 cut(s) 475, 505, 654
Hin1II CATG 5 cut(s) 304, 1451, 1481, 1700, 1733
Hin6I GCGC 3 cut(s) 473, 503, 652
HinP1I GCGC 3 cut(s) 473, 503, 652
HindIII AAGCTT 2 cut(s) 371, 1469
HpaII CCGG 4 cut(s) 486, 1286, 1328, 1427
HphI GGTGA 5 cut(s) 788, 1035, 1102, 1349, 1849
Hpy166II GTNNAC 1 cut(s) 1226
Hpy188I TCNGA 8 cut(s) 15, 468, 553, 623, 808, 1276, 1609, 1783
Hpy8I GTNNAC 1 cut(s) 1226
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 6 cut(s) 922, 974, 1209, 1358, 1508, 1799
HpyCH4III ACNGT 6 cut(s) 128, 272, 290, 357, 456, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 6 cut(s) 257, 347, 807, 1123, 1489, 1901
Hsp92II CATG 5 cut(s) 304, 1451, 1481, 1700, 1733
HspAI GCGC 3 cut(s) 473, 503, 652
Ksp22I TGATCA 1 cut(s) 1254
Kzo9I GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
LmnI GCTCC 4 cut(s) 29, 172, 397, 580
Lsp1109I GCAGC 7 cut(s) 316, 462, 517, 529, 717, 1636, 1698
LweI GCATC 6 cut(s) 554, 742, 1099, 1387, 1421, 1508
MaeI CTAG 6 cut(s) 122, 252, 309, 584, 1530, 1760
MaeIII GTNAC 4 cut(s) 950, 1118, 1484, 1837
MalI GATC 7 cut(s) 1042, 1094, 1136, 1219, 1256, 1389, 1780
MboI GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
MboII GAAGA 9 cut(s) 462, 540, 716, 728, 872, 1139, 1397, 1433, 1585
MflI RGATCY 2 cut(s) 1217, 1778
MhlI GDGCHC 3 cut(s) 34, 402, 806
MlyI GAGTC 5 cut(s) 19, 150, 633, 725, 1265
MmeI TCCRAC 3 cut(s) 339, 438, 1033
Mph1103I ATGCAT 3 cut(s) 1402, 1414, 1449
MroXI GAANNNNTTC 2 cut(s) 879, 1788
MseI TTAA 7 cut(s) 104, 497, 689, 791, 1155, 1302, 1725
MslI CAYNNNNRTG 1 cut(s) 1395
MspCI CTTAAG 1 cut(s) 688
MspI CCGG 4 cut(s) 486, 1286, 1328, 1427
MspR9I CCNGG 2 cut(s) 487, 1776
Mva1269I GAATGC 2 cut(s) 689, 1400
MvaI CCWGG 1 cut(s) 1776
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 487
NdeII GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
NlaIII CATG 5 cut(s) 304, 1451, 1481, 1700, 1733
NlaIV GGNNCC 3 cut(s) 1219, 1509, 1870
NmuCI GTSAC 2 cut(s) 1484, 1837
NsiI ATGCAT 3 cut(s) 1402, 1414, 1449
NspI RCATGY 2 cut(s) 1451, 1733
NspV TTCGAA 2 cut(s) 1320, 1851
PceI AGGCCT 1 cut(s) 935
PctI GAATGC 2 cut(s) 689, 1400
PdmI GAANNNNTTC 2 cut(s) 879, 1788
PfeI GAWTC 7 cut(s) 113, 178, 671, 1021, 1784, 1831, 1848
PflMI CCANNNNNTGG 2 cut(s) 169, 1865
PinAI ACCGGT 1 cut(s) 1426
PkrI GCNGC 7 cut(s) 331, 477, 507, 544, 732, 1626, 1713
PleI GAGTC 5 cut(s) 18, 149, 632, 724, 1265
PpsI GAGTC 5 cut(s) 18, 149, 632, 724, 1265
PpuMI RGGWCCY 2 cut(s) 1190, 1508
PshAI GACNNNNGTC 1 cut(s) 876
PsiI TTATAA 1 cut(s) 947
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 2 cut(s) 1190, 1508
Psp6I CCWGG 1 cut(s) 1774
PspGI CCWGG 1 cut(s) 1774
PspN4I GGNNCC 3 cut(s) 1219, 1509, 1870
PspPI GGNCC 3 cut(s) 1190, 1508, 1756
PspPPI RGGWCCY 2 cut(s) 1190, 1508
PstNI CAGNNNCTG 2 cut(s) 572, 1711
PsuI RGATCY 2 cut(s) 1217, 1778
RsaI GTAC 6 cut(s) 842, 996, 1164, 1179, 1227, 1899
RsaNI GTAC 6 cut(s) 841, 995, 1163, 1178, 1226, 1898
RseI CAYNNNNRTG 1 cut(s) 1395
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 7 cut(s) 104, 497, 689, 791, 1155, 1302, 1725
SatI GCNGC 7 cut(s) 330, 476, 506, 543, 731, 1625, 1712
Sau3AI GATC 7 cut(s) 1040, 1092, 1134, 1217, 1254, 1387, 1778
Sau96I GGNCC 3 cut(s) 1190, 1508, 1756
SchI GAGTC 5 cut(s) 19, 150, 633, 725, 1265
ScrFI CCNGG 2 cut(s) 487, 1776
SduI GDGCHC 3 cut(s) 34, 402, 806
SfaNI GCATC 6 cut(s) 554, 742, 1099, 1387, 1421, 1508
SfcI CTRYAG 2 cut(s) 1209, 1660
SfuI TTCGAA 2 cut(s) 1320, 1851
SinI GGWCC 3 cut(s) 1190, 1508, 1756
SmiMI CAYNNNNRTG 1 cut(s) 1395
SmlI CTYRAG 2 cut(s) 374, 688
SmoI CTYRAG 2 cut(s) 374, 688
SseBI AGGCCT 1 cut(s) 935
SsiI CCGC 1 cut(s) 1521
SspI AATATT 2 cut(s) 758, 1588
SspMI CTAG 6 cut(s) 122, 252, 309, 584, 1530, 1760
SstI GAGCTC 1 cut(s) 402
StuI AGGCCT 1 cut(s) 935
StyD4I CCNGG 2 cut(s) 485, 1774
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 6 cut(s) 128, 272, 290, 357, 456, 592
TaqI TCGA 9 cut(s) 111, 116, 1019, 1039, 1084, 1095, 1107, 1320, 1851
TaqII GACCGA 1 cut(s) 444
TatI WGTACW 3 cut(s) 1177, 1225, 1897
TfiI GAWTC 7 cut(s) 113, 178, 671, 1021, 1784, 1831, 1848
Tru1I TTAA 7 cut(s) 104, 497, 689, 791, 1155, 1302, 1725
Tru9I TTAA 7 cut(s) 104, 497, 689, 791, 1155, 1302, 1725
TscAI CASTG 5 cut(s) 806, 1228, 1368, 1380, 1712
TseFI GTSAC 2 cut(s) 1484, 1837
TseI GCWGC 7 cut(s) 329, 475, 505, 542, 730, 1624, 1711
Tsp45I GTSAC 2 cut(s) 1484, 1837
TspGWI ACGGA 2 cut(s) 1480, 1904
TspRI CASTG 5 cut(s) 806, 1228, 1368, 1380, 1712
Van91I CCANNNNNTGG 2 cut(s) 169, 1865
Vha464I CTTAAG 1 cut(s) 688
VpaK11BI GGWCC 3 cut(s) 1190, 1508, 1756
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 5 cut(s) 462, 1574, 1808, 1822, 1853
XceI RCATGY 2 cut(s) 1451, 1733
XcmI CCANNNNNNNNNTGG 1 cut(s) 859
XmnI GAANNNNTTC 2 cut(s) 879, 1788
XspI CTAG 6 cut(s) 122, 252, 309, 584, 1530, 1760
Zsp2I ATGCAT 3 cut(s) 1402, 1414, 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.