Rmu_sc0003419.1_g000031

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003419.1
OG0000408
Physical Location & Seq
Reverse (-)
103334 .. 107789
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003419.1_g000031.1.cds

Sequence Viewer

Length: 2874 bp
atggcagtggagttacttggaggagcagtgtttacactgctgtatgatggggttaagcaagcaattggaaagacagggactttcaaataccttctcaaagacatcaaatccacactggagtctttgaacaagctacacgccacggccatcagacaaatcggagagtataacctggaattgggtctcccaaatgatgatatagaagagctcaaaaagcaaatggaggaaggggcaaagctggtgggaaagttgtctgattttcgtgtgtggaactactgctgcttaaattgttataccgatcaacttgttgatttggataggtctctcaagaggctgttggagaatcttaagttgcaagaaactagagatgtgaaagagatcttggatttgctgaggaaataccgcgttgagcttgatgagttgaagggaacgttgcagatattactgcagcagaaagcaggagatgtgaaggagaccacctcgaattcaactgcaggaagcagtacagagaccatgcctcagcaaaatgtagggaacggagaggaacaaggaggggcaaatcttcgtgcagtgtatatgatgctgtttaacgttgttacagaagtgaaggacaagactttgatgttcagacccctactcggagatctcaagtccatgctagattccttggaaccactgatcaaagagatagatcaatacaatagcgtattggttctcccaaagccggaggtagaaaagttcaggacacaaatggacaagggtgtggagctcgttcgcaagtgctccaaggttcgtccgtggaccagctacaaaaagtacaattacacaatccgacttattggattgaatgaatctgttcgattactgttaagtagactgagagagcaggtagctggggaggtgagtgagacattggcttcaataagtaacctaaaggaggtaatcaagcaatatgaagggcctagtgtcatacaaaatcaacatgagattataggttcctgtaaagtacctgaaccttcatcgcctacattgatgcctgagattgagggttctgatgaagtgcatccattaccagtgcctcctggagcaaaactaccttcaccaaaggaggaagttggggaagtgagcgagacattggcttcaataagtaacctaaaggaggtagtcaagcaatatgaagggcctagtggcatacaaaatcaacatgagattataggttcctgcaaagtacccgaacattcatcgcctacagggatgcctgagattgaggattctgttgaagtgcatccattaccagtgcctcctggagcaaaactaccttcaccaaaggttctaattacatctgaaccggagtcccagccaatgaaaggtcaatggcaaaagggaaatcttattggtcgtggcacgtttgggaatgtttatgttgcttccaatggagaaactgcagctttatgtgcaatgaaggaagttgatttcattcctgatgacccaaaatctgccgagtgtatgaagcagctacagcaggaaatcaaagtcctcagccggctcaagcatcctaatattgtgcaatattatgggaccgagatagttggagatagattttttatatatcaggagtatgtccatcctgtatctattaacagatacatccaagaatattgtggagccatgacagaagctgttgttcgcagtttaactcaccagatagtgtctgggttggcttacttgcacagcacagaaacgatacacggggacatcaaaggggataatttgctcgttgagtcatctggtgttgtcaagatagctgactttggaattgtaaaacatcttcgaggaagactcctaaagggaggtccatactggatggctccagagatcatcaaaaatgggataccgaacgataaatgctctgatcatgatttggctcgtggtggtgacatatggagtttgggttgcactgttattgaattgttcactggaaaacctccctggagtgagttcgaacaggttcaagccatgtttctaattgttcgtaaagatcctccaattccagaaacattgtcacctgagggtaaggatttcctaacatgctgcttgcagagaaatcctgcagagaggccaactgctgcaatgcttttagaacatccattcttaaagaactcccagccgcaggatctcccaactatgatcggtgccattaatctggtgaatttgatggataaacacaaaatgacggaactccaatcgaacttgaaagaagacttgatcgttgagaagatcaggttctccactgatccatcaatccatcactgcgggatgtctctcgaaagaggcaaggaattctcaggaggagaaggatcgtcattggggtactcgagtccgagtcccacagatcatcatgaccagcagcagcagccagtgacgctcagcaggcatgagatgcagaagaggagggactggaacactttcgggcagtacttgaagaaccagaggcccccagtgcctccctcccagtgcaattacagccatgttctcgagttcctcaggtacctcgaccagttcgggaagaccaaagtccatctccagggttgtgtgttttacggccagccggagccacctgcgccttgcacctgtcctctccggcaggcctggggaagcctggaagctctcatagggaggctgagggctgcttatgaggagaacggtggggcccccgagagcaatccttttgctagtggtgcaatcagggggtacttgagggaggtcagggagtatcaggccaaggctagagggattccttacaataataagaagaagaagcctggaaatagtgatcgtgattcttcaacaagttctatcatgcacttcacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

957

Amino Acids

106.96

Weight (kDa)

6.15

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2626
Acc36I ACCTGC 2 cut(s) 877, 2626
Acc65I GGTACC 1 cut(s) 2547
AccB1I GGYRCC 2 cut(s) 2192, 2547
AccI GTMKAC 1 cut(s) 874
AccII CGCG 1 cut(s) 405
AciI CCGC 3 cut(s) 403, 2168, 2313
AclI AACGTT 2 cut(s) 431, 591
AclWI GGATC 4 cut(s) 2033, 2181, 2288, 2365
AcoI YGGCCR 2 cut(s) 144, 2602
AcsI RAATTY 3 cut(s) 484, 2209, 2339
AfaI GTAC 8 cut(s) 505, 818, 1008, 1230, 2372, 2477, 2549, 2753
AfiI CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2170, 2584
AflII CTTAAG 1 cut(s) 347
AjnI CCWGG 8 cut(s) 171, 1081, 1303, 1988, 2583, 2648, 2658, 2821
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
Alw21I GWGCWC 3 cut(s) 210, 771, 785
Alw26I GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2325
AlwI GGATC 4 cut(s) 2033, 2181, 2288, 2365
AlwNI CAGNNNCTG 1 cut(s) 1679
Ama87I CYCGRG 3 cut(s) 2374, 2534, 2714
AoxI GGCC 9 cut(s) 144, 959, 1181, 2117, 2492, 2602, 2646, 2709, 2778
ApaI GGGCCC 1 cut(s) 2713
ApoI RAATTY 3 cut(s) 484, 2209, 2339
AseI ATTAAT 1 cut(s) 2199
Asp700I GAANNNNTTC 3 cut(s) 855, 2007, 2465
Asp718I GGTACC 1 cut(s) 2547
AspLEI GCGC 1 cut(s) 2623
AspS9I GGNCC 8 cut(s) 801, 959, 1181, 1578, 1853, 2493, 2709, 2710
AsuHPI GGTGA 7 cut(s) 913, 1092, 1314, 1691, 1946, 2055, 2218
AsuII TTCGAA 1 cut(s) 2001
AvaI CYCGRG 3 cut(s) 2374, 2534, 2714
AvaII GGWCC 3 cut(s) 801, 1578, 1853
AxyI CCTNAGG 2 cut(s) 2067, 2543
BaeGI GKGCMC 1 cut(s) 2713
BaeI ACNNNNGTAYC 2 cut(s) 2362, 2395
BanI GGYRCC 2 cut(s) 2192, 2547
BanII GRGCYC 3 cut(s) 210, 771, 2713
BarI GAAGNNNNNNTAC 2 cut(s) 1812, 1844
BauI CACGAG 1 cut(s) 1926
BbsI GAAGAC 3 cut(s) 1843, 2265, 2573
Bbv12I GWGCWC 3 cut(s) 210, 771, 785
BbvCI CCTCAGC 4 cut(s) 392, 519, 1538, 2681
BccI CCATC 8 cut(s) 41, 155, 1632, 1858, 2209, 2305, 2313, 2586
BceAI ACGGC 2 cut(s) 159, 2617
BciT130I CCWGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
BciVI GTATCC 1 cut(s) 1884
BclI TGATCA 2 cut(s) 678, 1912
BcoDI GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2325
BfaI CTAG 6 cut(s) 363, 659, 963, 1185, 2733, 2787
BfmI CTRYAG 6 cut(s) 446, 492, 1248, 1443, 1517, 2109
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 2 cut(s) 877, 2626
BfuI GTATCC 1 cut(s) 1884
BglII AGATCT 2 cut(s) 378, 643
BlpI GCTNAGC 1 cut(s) 2426
BmcAI AGTACT 1 cut(s) 2477
Bme1390I CCNGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
Bme18I GGWCC 3 cut(s) 801, 1578, 1853
BmeT110I CYCGRG 3 cut(s) 2374, 2534, 2714
BmgT120I GGNCC 8 cut(s) 801, 959, 1181, 1578, 1853, 2493, 2709, 2710
BmrFI CCNGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
BmrI ACTGGG 2 cut(s) 2492, 2506
BmsI GCATC 7 cut(s) 570, 1023, 1072, 1245, 1294, 1561, 2430
BmuI ACTGGG 2 cut(s) 2492, 2506
BoxI GACNNNNGTC 1 cut(s) 2573
BpiI GAAGAC 3 cut(s) 1843, 2265, 2573
BplI GAGNNNNNCTC 4 cut(s) 464, 496, 1824, 1856
BpmI CTGGAG 6 cut(s) 137, 1104, 1326, 1854, 2011, 2567
Bpu10I CCTNAGC 4 cut(s) 392, 519, 1538, 2681
Bpu1102I GCTNAGC 1 cut(s) 2426
Bpu14I TTCGAA 1 cut(s) 2001
BpuEI CTTGAG 4 cut(s) 311, 632, 1532, 2776
BsaI GGTCTC 4 cut(s) 188, 327, 467, 503
BsaJI CCNNGG 8 cut(s) 141, 666, 786, 797, 1988, 2584, 2649, 2781
BsaWI WCCGGW 1 cut(s) 1348
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 1936, 1966
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2170, 2584
Bse118I RCCGGY 1 cut(s) 1542
Bse21I CCTNAGG 2 cut(s) 2067, 2543
Bse3DI GCAATG 2 cut(s) 1464, 2136
BseBI CCWGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
BseDI CCNNGG 8 cut(s) 141, 666, 786, 797, 1988, 2584, 2649, 2781
BseGI GGATG 9 cut(s) 1063, 1260, 1285, 1552, 1624, 1647, 1869, 2143, 2322
BseLI CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2170, 2584
BseMI GCAATG 2 cut(s) 1464, 2136
BseRI GAGGAG 4 cut(s) 36, 2364, 2464, 2711
BseSI GKGCMC 1 cut(s) 2713
BseYI CCCAGC 3 cut(s) 893, 1356, 2163
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 9 cut(s) 146, 961, 1183, 2119, 2494, 2604, 2648, 2711, 2780
BshNI GGYRCC 2 cut(s) 2192, 2547
BsiHKAI GWGCWC 3 cut(s) 210, 771, 785
BsiHKCI CYCGRG 3 cut(s) 2374, 2534, 2714
BsiSI CCGG 5 cut(s) 725, 1349, 1543, 2609, 2641
BslFI GGGAC 6 cut(s) 91, 1339, 1591, 1766, 2370, 2468
BslI CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2170, 2584
BsmAI GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2325
BsmFI GGGAC 6 cut(s) 91, 1339, 1591, 1766, 2370, 2468
BsnI GGCC 9 cut(s) 146, 961, 1183, 2119, 2494, 2604, 2648, 2711, 2780
Bso31I GGTCTC 4 cut(s) 188, 327, 467, 503
BsoBI CYCGRG 3 cut(s) 2374, 2534, 2714
Bsp119I TTCGAA 1 cut(s) 2001
Bsp120I GGGCCC 1 cut(s) 2709
Bsp1286I GDGCHC 4 cut(s) 210, 771, 785, 2713
Bsp1720I GCTNAGC 1 cut(s) 2426
BspACI CCGC 3 cut(s) 403, 2168, 2313
BspANI GGCC 9 cut(s) 146, 961, 1183, 2119, 2494, 2604, 2648, 2711, 2780
BspFNI CGCG 1 cut(s) 405
BspHI TCATGA 2 cut(s) 1915, 2398
BspMAI CTGCAG 4 cut(s) 450, 496, 1447, 2113
BspMI ACCTGC 2 cut(s) 877, 2626
BspPI GGATC 4 cut(s) 2033, 2181, 2288, 2365
BspQI GCTCTTC 1 cut(s) 198
BspT104I TTCGAA 1 cut(s) 2001
BspT107I GGYRCC 2 cut(s) 2192, 2547
BspTI CTTAAG 1 cut(s) 347
BspTNI GGTCTC 4 cut(s) 188, 327, 467, 503
BsrDI GCAATG 2 cut(s) 1464, 2136
BsrFI RCCGGY 1 cut(s) 1542
BssAI RCCGGY 1 cut(s) 1542
BssECI CCNNGG 8 cut(s) 141, 666, 786, 797, 1988, 2584, 2649, 2781
BssSI CACGAG 1 cut(s) 1926
BssT1I CCWWGG 3 cut(s) 666, 786, 2781
Bst2BI CACGAG 1 cut(s) 1926
Bst2UI CCWGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
Bst4CI ACNGT 3 cut(s) 867, 1960, 2705
Bst6I CTCTTC 2 cut(s) 198, 2441
BstAFI CTTAAG 1 cut(s) 347
BstAPI GCANNNNNTGC 1 cut(s) 2440
BstBI TTCGAA 1 cut(s) 2001
BstC8I GCNNGC 6 cut(s) 60, 1544, 2096, 2432, 2606, 2646
BstDSI CCRYGG 2 cut(s) 141, 797
BstENI CCTNNNNNAGG 2 cut(s) 935, 1157
BstF5I GGATG 9 cut(s) 1063, 1260, 1285, 1552, 1624, 1647, 1869, 2143, 2322
BstFNI CGCG 1 cut(s) 405
BstHHI GCGC 1 cut(s) 2623
BstMAI GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2325
BstNI CCWGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
BstNSI RCATGY 1 cut(s) 2091
BstPAI GACNNNNGTC 1 cut(s) 2573
BstSCI CCNGG 8 cut(s) 171, 1081, 1303, 1988, 2583, 2648, 2658, 2821
BstSFI CTRYAG 6 cut(s) 446, 492, 1248, 1443, 1517, 2109
BstSLI GKGCMC 1 cut(s) 2713
BstUI CGCG 1 cut(s) 405
BstV2I GAAGAC 3 cut(s) 1843, 2265, 2573
BstX2I RGATCY 4 cut(s) 378, 643, 2038, 2173
BstXI CCANNNNNNTGG 1 cut(s) 2203
BstYI RGATCY 4 cut(s) 378, 643, 2038, 2173
Bsu36I CCTNAGG 2 cut(s) 2067, 2543
BsuI GTATCC 1 cut(s) 1884
BsuRI GGCC 9 cut(s) 146, 961, 1183, 2119, 2494, 2604, 2648, 2711, 2780
BtgI CCRYGG 2 cut(s) 141, 797
BtgZI GCGATG 2 cut(s) 1005, 1227
BtsCI GGATG 9 cut(s) 1063, 1260, 1285, 1552, 1624, 1647, 1869, 2143, 2322
BtsI GCAGTG 5 cut(s) 12, 33, 35, 576, 2308
BveI ACCTGC 2 cut(s) 877, 2626
Cac8I GCNNGC 6 cut(s) 60, 1544, 2096, 2432, 2606, 2646
CaiI CAGNNNCTG 1 cut(s) 1679
CciI TCATGA 2 cut(s) 1915, 2398
CfoI GCGC 1 cut(s) 2623
Cfr10I RCCGGY 1 cut(s) 1542
Cfr13I GGNCC 8 cut(s) 801, 959, 1181, 1578, 1853, 2493, 2709, 2710
CseI GACGC 1 cut(s) 2431
Csp6I GTAC 8 cut(s) 504, 817, 1007, 1229, 2371, 2476, 2548, 2752
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviQI GTAC 8 cut(s) 504, 817, 1007, 1229, 2371, 2476, 2548, 2752
EaeI YGGCCR 2 cut(s) 144, 2602
Eam1104I CTCTTC 2 cut(s) 198, 2441
EarI CTCTTC 2 cut(s) 198, 2441
Ecl136II GAGCTC 2 cut(s) 208, 769
Eco130I CCWWGG 3 cut(s) 666, 786, 2781
Eco147I AGGCCT 1 cut(s) 2648
Eco24I GRGCYC 3 cut(s) 210, 771, 2713
Eco31I GGTCTC 4 cut(s) 188, 327, 467, 503
Eco47I GGWCC 3 cut(s) 801, 1578, 1853
Eco53kI GAGCTC 2 cut(s) 208, 769
Eco81I CCTNAGG 2 cut(s) 2067, 2543
Eco88I CYCGRG 3 cut(s) 2374, 2534, 2714
EcoICRI GAGCTC 2 cut(s) 208, 769
EcoNI CCTNNNNNAGG 2 cut(s) 935, 1157
EcoO109I RGGNCCY 5 cut(s) 959, 1181, 2493, 2709, 2710
EcoRI GAATTC 2 cut(s) 484, 2339
EcoRII CCWGG 8 cut(s) 171, 1081, 1303, 1988, 2583, 2648, 2658, 2821
EcoT14I CCWWGG 3 cut(s) 666, 786, 2781
EcoT38I GRGCYC 3 cut(s) 210, 771, 2713
ErhI CCWWGG 3 cut(s) 666, 786, 2781
FaqI GGGAC 6 cut(s) 91, 1339, 1591, 1766, 2370, 2468
FauI CCCGC 1 cut(s) 2306
FauNDI CATATG 1 cut(s) 1940
FbaI TGATCA 2 cut(s) 678, 1912
FblI GTMKAC 1 cut(s) 874
FokI GGATG 9 cut(s) 1050, 1267, 1272, 1539, 1611, 1634, 1876, 2130, 2329
FriOI GRGCYC 3 cut(s) 210, 771, 2713
FspBI CTAG 6 cut(s) 363, 659, 963, 1185, 2733, 2787
GlaI GCGC 1 cut(s) 2622
GsaI CCCAGC 3 cut(s) 897, 1360, 2167
GsuI CTGGAG 6 cut(s) 137, 1104, 1326, 1854, 2011, 2567
HaeIII GGCC 9 cut(s) 146, 961, 1183, 2119, 2494, 2604, 2648, 2711, 2780
HapII CCGG 5 cut(s) 725, 1349, 1543, 2609, 2641
HgaI GACGC 1 cut(s) 2431
HhaI GCGC 1 cut(s) 2623
Hin6I GCGC 1 cut(s) 2621
HinP1I GCGC 1 cut(s) 2621
HpaII CCGG 5 cut(s) 725, 1349, 1543, 2609, 2641
HphI GGTGA 7 cut(s) 913, 1092, 1314, 1691, 1946, 2055, 2218
Hpy166II GTNNAC 4 cut(s) 33, 801, 875, 1974
Hpy8I GTNNAC 4 cut(s) 33, 801, 875, 1974
HpyCH4III ACNGT 3 cut(s) 867, 1960, 2705
HpyCH4IV ACGT 3 cut(s) 431, 591, 1406
HpySE526I ACGT 3 cut(s) 431, 591, 1406
HspAI GCGC 1 cut(s) 2621
KpnI GGTACC 1 cut(s) 2551
KroI GCCGGC 1 cut(s) 1542
KroNI GCCGGC 1 cut(s) 1544
Ksp22I TGATCA 2 cut(s) 678, 1912
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 8 cut(s) 23, 766, 788, 1085, 1307, 1664, 1873, 2611
LweI GCATC 7 cut(s) 570, 1023, 1072, 1245, 1294, 1561, 2430
MaeI CTAG 6 cut(s) 363, 659, 963, 1185, 2733, 2787
MaeII ACGT 3 cut(s) 431, 591, 1406
MaeIII GTNAC 7 cut(s) 12, 595, 926, 1148, 1934, 2061, 2419
MfeI CAATTG 1 cut(s) 63
MflI RGATCY 4 cut(s) 378, 643, 2038, 2173
MhlI GDGCHC 4 cut(s) 210, 771, 785, 2713
MlyI GAGTC 6 cut(s) 128, 1361, 1790, 1833, 2386, 2392
MmeI TCCRAC 3 cut(s) 318, 856, 1570
MroNI GCCGGC 1 cut(s) 1542
MroXI GAANNNNTTC 3 cut(s) 855, 2007, 2465
MseI TTAA 9 cut(s) 54, 284, 348, 588, 869, 1638, 1694, 2153, 2199
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 5 cut(s) 725, 1349, 1543, 2609, 2641
MspR9I CCNGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
MunI CAATTG 1 cut(s) 63
MvaI CCWGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
MvnI CGCG 1 cut(s) 405
NaeI GCCGGC 1 cut(s) 1544
NdeI CATATG 1 cut(s) 1940
NgoMIV GCCGGC 1 cut(s) 1542
NmeAIII GCCGAG 1 cut(s) 1525
NmuCI GTSAC 3 cut(s) 1934, 2061, 2419
NspI RCATGY 1 cut(s) 2091
NspV TTCGAA 1 cut(s) 2001
PaeR7I CTCGAG 2 cut(s) 2374, 2534
PagI TCATGA 2 cut(s) 1915, 2398
PaqCI CACCTGC 1 cut(s) 2626
PceI AGGCCT 1 cut(s) 2648
PciSI GCTCTTC 1 cut(s) 198
PdiI GCCGGC 1 cut(s) 1544
PdmI GAANNNNTTC 3 cut(s) 855, 2007, 2465
PfeI GAWTC 6 cut(s) 343, 662, 851, 1271, 2794, 2840
PfoI TCCNGGA 2 cut(s) 1081, 1303
PleI GAGTC 6 cut(s) 127, 1360, 1789, 1833, 2385, 2391
PpsI GAGTC 6 cut(s) 127, 1360, 1789, 1833, 2385, 2391
PshAI GACNNNNGTC 1 cut(s) 2573
PshBI ATTAAT 1 cut(s) 2199
Psp124BI GAGCTC 2 cut(s) 210, 771
Psp1406I AACGTT 2 cut(s) 431, 591
Psp6I CCWGG 8 cut(s) 171, 1081, 1303, 1988, 2583, 2648, 2658, 2821
PspFI CCCAGC 3 cut(s) 893, 1356, 2163
PspGI CCWGG 8 cut(s) 171, 1081, 1303, 1988, 2583, 2648, 2658, 2821
PspOMI GGGCCC 1 cut(s) 2709
PspPI GGNCC 8 cut(s) 801, 959, 1181, 1578, 1853, 2493, 2709, 2710
PspXI VCTCGAGB 1 cut(s) 2374
PstI CTGCAG 4 cut(s) 450, 496, 1447, 2113
PstNI CAGNNNCTG 1 cut(s) 1679
PsuI RGATCY 4 cut(s) 378, 643, 2038, 2173
RsaI GTAC 8 cut(s) 505, 818, 1008, 1230, 2372, 2477, 2549, 2753
RsaNI GTAC 8 cut(s) 504, 817, 1007, 1229, 2371, 2476, 2548, 2752
SacI GAGCTC 2 cut(s) 210, 771
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 9 cut(s) 54, 284, 348, 588, 869, 1638, 1694, 2153, 2199
Sau96I GGNCC 8 cut(s) 801, 959, 1181, 1578, 1853, 2493, 2709, 2710
ScaI AGTACT 1 cut(s) 2477
SchI GAGTC 6 cut(s) 128, 1361, 1790, 1833, 2386, 2392
ScrFI CCNGG 8 cut(s) 173, 1083, 1305, 1990, 2585, 2650, 2660, 2823
SduI GDGCHC 4 cut(s) 210, 771, 785, 2713
SfaNI GCATC 7 cut(s) 570, 1023, 1072, 1245, 1294, 1561, 2430
SfcI CTRYAG 6 cut(s) 446, 492, 1248, 1443, 1517, 2109
Sfr274I CTCGAG 2 cut(s) 2374, 2534
SfuI TTCGAA 1 cut(s) 2001
SinI GGWCC 3 cut(s) 801, 1578, 1853
SlaI CTCGAG 2 cut(s) 2374, 2534
SmlI CTYRAG 7 cut(s) 326, 347, 647, 1547, 2374, 2534, 2755
SmoI CTYRAG 7 cut(s) 326, 347, 647, 1547, 2374, 2534, 2755
SseBI AGGCCT 1 cut(s) 2648
SsiI CCGC 3 cut(s) 403, 2168, 2313
SspI AATATT 3 cut(s) 1561, 1571, 1658
SspMI CTAG 6 cut(s) 363, 659, 963, 1185, 2733, 2787
SstI GAGCTC 2 cut(s) 210, 771
StuI AGGCCT 1 cut(s) 2648
StyD4I CCNGG 8 cut(s) 171, 1081, 1303, 1988, 2583, 2648, 2658, 2821
StyI CCWWGG 3 cut(s) 666, 786, 2781
TaaI ACNGT 3 cut(s) 867, 1960, 2705
TaiI ACGT 3 cut(s) 434, 594, 1409
TaqI TCGA 9 cut(s) 482, 859, 1831, 2001, 2246, 2325, 2375, 2535, 2553
TaqII GACCGA 1 cut(s) 1595
TatI WGTACW 3 cut(s) 503, 816, 2475
TauI GCSGC 1 cut(s) 2170
TfiI GAWTC 6 cut(s) 343, 662, 851, 1271, 2794, 2840
Tru1I TTAA 9 cut(s) 54, 284, 348, 588, 869, 1638, 1694, 2153, 2199
Tru9I TTAA 9 cut(s) 54, 284, 348, 588, 869, 1638, 1694, 2153, 2199
TseFI GTSAC 3 cut(s) 1934, 2061, 2419
Tsp45I GTSAC 3 cut(s) 1934, 2061, 2419
TspGWI ACGGA 3 cut(s) 552, 786, 2249
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 3 cut(s) 801, 1578, 1853
VspI ATTAAT 1 cut(s) 2199
XagI CCTNNNNNAGG 2 cut(s) 935, 1157
XapI RAATTY 3 cut(s) 484, 2209, 2339
XceI RCATGY 1 cut(s) 2091
XcmI CCANNNNNNNNNTGG 2 cut(s) 1658, 1709
XhoI CTCGAG 2 cut(s) 2374, 2534
XmiI GTMKAC 1 cut(s) 874
XmnI GAANNNNTTC 3 cut(s) 855, 2007, 2465
XspI CTAG 6 cut(s) 363, 659, 963, 1185, 2733, 2787
ZrmI AGTACT 1 cut(s) 2477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.