protein serine/threonine kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 141 |
| AccBSI | CCGCTC | 1 cut(s) | 515 |
| AccII | CGCG | 1 cut(s) | 735 |
| AccIII | TCCGGA | 1 cut(s) | 494 |
| AciI | CCGC | 5 cut(s) | 21, 51, 513, 956, 981 |
| AclWI | GGATC | 1 cut(s) | 816 |
| AcsI | RAATTY | 4 cut(s) | 11, 247, 484, 999 |
| AcuI | CTGAAG | 1 cut(s) | 921 |
| AcyI | GRCGYC | 2 cut(s) | 138, 609 |
| AfaI | GTAC | 3 cut(s) | 129, 505, 1015 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 519 |
| AflIII | ACRYGT | 1 cut(s) | 946 |
| AgsI | TTSAA | 8 cut(s) | 152, 340, 584, 701, 878, 974, 992, 1004 |
| AjnI | CCWGG | 1 cut(s) | 675 |
| AluBI | AGCT | 6 cut(s) | 229, 458, 575, 787, 825, 853 |
| AluI | AGCT | 6 cut(s) | 229, 458, 575, 787, 825, 853 |
| Alw21I | GWGCWC | 2 cut(s) | 789, 803 |
| Alw26I | GTCTC | 3 cut(s) | 93, 129, 179 |
| AlwI | GGATC | 1 cut(s) | 816 |
| Aor13HI | TCCGGA | 1 cut(s) | 494 |
| AoxI | GGCC | 1 cut(s) | 819 |
| ApeKI | GCWGC | 5 cut(s) | 37, 75, 328, 448, 825 |
| ApoI | RAATTY | 4 cut(s) | 11, 247, 484, 999 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 873 |
| AspLEI | GCGC | 1 cut(s) | 737 |
| AspS9I | GGNCC | 1 cut(s) | 819 |
| AsuHPI | GGTGA | 1 cut(s) | 970 |
| AsuII | TTCGAA | 1 cut(s) | 253 |
| BanII | GRGCYC | 2 cut(s) | 316, 789 |
| BbsI | GAAGAC | 1 cut(s) | 427 |
| Bbv12I | GWGCWC | 2 cut(s) | 789, 803 |
| BbvI | GCAGC | 5 cut(s) | 24, 87, 315, 460, 812 |
| BccI | CCATC | 1 cut(s) | 205 |
| BceAI | ACGGC | 2 cut(s) | 64, 596 |
| BciT130I | CCWGG | 1 cut(s) | 677 |
| BclI | TGATCA | 1 cut(s) | 960 |
| BcoDI | GTCTC | 3 cut(s) | 93, 129, 179 |
| BfaI | CTAG | 2 cut(s) | 8, 125 |
| BfmI | CTRYAG | 1 cut(s) | 446 |
| BisI | GCNGC | 5 cut(s) | 38, 76, 329, 449, 826 |
| BlsI | GCNGC | 5 cut(s) | 39, 77, 330, 450, 827 |
| Bme1390I | CCNGG | 1 cut(s) | 677 |
| BmgT120I | GGNCC | 1 cut(s) | 819 |
| BmrFI | CCNGG | 1 cut(s) | 677 |
| BmsI | GCATC | 1 cut(s) | 333 |
| BpiI | GAAGAC | 1 cut(s) | 427 |
| Bpu14I | TTCGAA | 1 cut(s) | 253 |
| BsaHI | GRCGYC | 2 cut(s) | 138, 609 |
| BsaI | GGTCTC | 2 cut(s) | 93, 179 |
| BsaJI | CCNNGG | 3 cut(s) | 518, 675, 804 |
| BsaWI | WCCGGW | 1 cut(s) | 494 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 519 |
| Bse1I | ACTGG | 1 cut(s) | 120 |
| Bse3DI | GCAATG | 1 cut(s) | 1067 |
| BseAI | TCCGGA | 1 cut(s) | 494 |
| BseBI | CCWGG | 1 cut(s) | 677 |
| BseDI | CCNNGG | 3 cut(s) | 518, 675, 804 |
| BseGI | GGATG | 4 cut(s) | 364, 412, 871, 1060 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 519 |
| BseMI | GCAATG | 1 cut(s) | 1067 |
| BseMII | CTCAG | 1 cut(s) | 871 |
| BseNI | ACTGG | 1 cut(s) | 120 |
| BseRI | GAGGAG | 2 cut(s) | 585, 755 |
| BseXI | GCAGC | 5 cut(s) | 24, 87, 315, 460, 812 |
| BseYI | CCCAGC | 1 cut(s) | 458 |
| BsgI | GTGCAG | 1 cut(s) | 1061 |
| Bsh1236I | CGCG | 1 cut(s) | 735 |
| BshFI | GGCC | 1 cut(s) | 821 |
| BsiHKAI | GWGCWC | 2 cut(s) | 789, 803 |
| BsiSI | CCGG | 1 cut(s) | 495 |
| BslFI | GGGAC | 1 cut(s) | 641 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 519 |
| BsmAI | GTCTC | 3 cut(s) | 93, 129, 179 |
| BsmBI | CGTCTC | 1 cut(s) | 129 |
| BsmFI | GGGAC | 1 cut(s) | 641 |
| BsnI | GGCC | 1 cut(s) | 821 |
| Bso31I | GGTCTC | 2 cut(s) | 93, 179 |
| Bsp119I | TTCGAA | 1 cut(s) | 253 |
| Bsp1286I | GDGCHC | 3 cut(s) | 316, 789, 803 |
| Bsp13I | TCCGGA | 1 cut(s) | 494 |
| Bsp143I | GATC | 3 cut(s) | 730, 808, 960 |
| BspACI | CCGC | 5 cut(s) | 21, 51, 513, 956, 981 |
| BspANI | GGCC | 1 cut(s) | 821 |
| BspCNI | CTCAG | 1 cut(s) | 870 |
| BspEI | TCCGGA | 1 cut(s) | 494 |
| BspFNI | CGCG | 1 cut(s) | 735 |
| BspMAI | CTGCAG | 1 cut(s) | 450 |
| BspPI | GGATC | 1 cut(s) | 816 |
| BspQI | GCTCTTC | 1 cut(s) | 147 |
| BspT104I | TTCGAA | 1 cut(s) | 253 |
| BspTNI | GGTCTC | 2 cut(s) | 93, 179 |
| BsrBI | CCGCTC | 1 cut(s) | 515 |
| BsrDI | GCAATG | 1 cut(s) | 1067 |
| BsrI | ACTGG | 1 cut(s) | 120 |
| BssECI | CCNNGG | 3 cut(s) | 518, 675, 804 |
| BssMI | GATC | 3 cut(s) | 730, 808, 960 |
| BssNI | GRCGYC | 2 cut(s) | 138, 609 |
| BssT1I | CCWWGG | 1 cut(s) | 804 |
| Bst2UI | CCWGG | 1 cut(s) | 677 |
| Bst4CI | ACNGT | 5 cut(s) | 426, 637, 654, 885, 1018 |
| Bst6I | CTCTTC | 2 cut(s) | 147, 696 |
| BstACI | GRCGYC | 2 cut(s) | 138, 609 |
| BstBI | TTCGAA | 1 cut(s) | 253 |
| BstC8I | GCNNGC | 2 cut(s) | 60, 823 |
| BstDEI | CTNAG | 2 cut(s) | 857, 1101 |
| BstDSI | CCRYGG | 1 cut(s) | 518 |
| BstF5I | GGATG | 4 cut(s) | 364, 412, 871, 1060 |
| BstFNI | CGCG | 1 cut(s) | 735 |
| BstHHI | GCGC | 1 cut(s) | 737 |
| BstKTI | GATC | 3 cut(s) | 733, 811, 963 |
| BstMAI | GTCTC | 3 cut(s) | 93, 129, 179 |
| BstMBI | GATC | 3 cut(s) | 730, 808, 960 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 34, 75, 1039 |
| BstNI | CCWGG | 1 cut(s) | 677 |
| BstNSI | RCATGY | 1 cut(s) | 950 |
| BstSCI | CCNGG | 1 cut(s) | 675 |
| BstSFI | CTRYAG | 1 cut(s) | 446 |
| BstUI | CGCG | 1 cut(s) | 735 |
| BstV1I | GCAGC | 5 cut(s) | 24, 87, 315, 460, 812 |
| BstV2I | GAAGAC | 1 cut(s) | 427 |
| BsuRI | GGCC | 1 cut(s) | 821 |
| BtgI | CCRYGG | 1 cut(s) | 518 |
| BtgZI | GCGATG | 1 cut(s) | 77 |
| BtsCI | GGATG | 4 cut(s) | 364, 412, 871, 1060 |
| BtsI | GCAGTG | 1 cut(s) | 594 |
| BtsIMutI | CAGTG | 5 cut(s) | 113, 594, 650, 843, 945 |
| Cac8I | GCNNGC | 2 cut(s) | 60, 823 |
| CfoI | GCGC | 1 cut(s) | 737 |
| Cfr13I | GGNCC | 1 cut(s) | 819 |
| CseI | GACGC | 1 cut(s) | 617 |
| Csp6I | GTAC | 3 cut(s) | 128, 504, 1014 |
| CviAII | CATG | 1 cut(s) | 947 |
| CviQI | GTAC | 3 cut(s) | 128, 504, 1014 |
| DdeI | CTNAG | 2 cut(s) | 857, 1101 |
| DpnI | GATC | 3 cut(s) | 732, 810, 962 |
| DpnII | GATC | 3 cut(s) | 730, 808, 960 |
| DraI | TTTAAA | 2 cut(s) | 85, 646 |
| Eam1104I | CTCTTC | 2 cut(s) | 147, 696 |
| EarI | CTCTTC | 2 cut(s) | 147, 696 |
| EciI | GGCGGA | 1 cut(s) | 36 |
| Ecl136II | GAGCTC | 1 cut(s) | 787 |
| Eco130I | CCWWGG | 1 cut(s) | 804 |
| Eco24I | GRGCYC | 2 cut(s) | 316, 789 |
| Eco31I | GGTCTC | 2 cut(s) | 93, 179 |
| Eco53kI | GAGCTC | 1 cut(s) | 787 |
| Eco57I | CTGAAG | 1 cut(s) | 921 |
| EcoICRI | GAGCTC | 1 cut(s) | 787 |
| EcoRII | CCWGG | 1 cut(s) | 675 |
| EcoT14I | CCWWGG | 1 cut(s) | 804 |
| EcoT38I | GRGCYC | 2 cut(s) | 316, 789 |
| ErhI | CCWWGG | 1 cut(s) | 804 |
| Esp3I | CGTCTC | 1 cut(s) | 129 |
| FaeI | CATG | 1 cut(s) | 950 |
| FaqI | GGGAC | 1 cut(s) | 641 |
| FatI | CATG | 1 cut(s) | 946 |
| FbaI | TGATCA | 1 cut(s) | 960 |
| Fnu4HI | GCNGC | 5 cut(s) | 38, 76, 329, 449, 826 |
| FokI | GGATG | 4 cut(s) | 371, 419, 878, 1067 |
| FriOI | GRGCYC | 2 cut(s) | 316, 789 |
| Fsp4HI | GCNGC | 5 cut(s) | 38, 76, 329, 449, 826 |
| FspBI | CTAG | 2 cut(s) | 8, 125 |
| GlaI | GCGC | 1 cut(s) | 736 |
| GluI | GCNGC | 5 cut(s) | 38, 76, 329, 449, 826 |
| GsaI | CCCAGC | 1 cut(s) | 462 |
| HaeIII | GGCC | 1 cut(s) | 821 |
| HapII | CCGG | 1 cut(s) | 495 |
| HgaI | GACGC | 1 cut(s) | 617 |
| HhaI | GCGC | 1 cut(s) | 737 |
| Hin1I | GRCGYC | 2 cut(s) | 138, 609 |
| Hin1II | CATG | 1 cut(s) | 950 |
| Hin6I | GCGC | 1 cut(s) | 735 |
| HinP1I | GCGC | 1 cut(s) | 735 |
| HindIII | AAGCTT | 1 cut(s) | 851 |
| HinfI | GANTC | 4 cut(s) | 119, 680, 869, 1064 |
| HpaII | CCGG | 1 cut(s) | 495 |
| HphI | GGTGA | 1 cut(s) | 970 |
| Hpy166II | GTNNAC | 2 cut(s) | 33, 415 |
| Hpy188I | TCNGA | 1 cut(s) | 860 |
| Hpy188III | TCNNGA | 3 cut(s) | 495, 581, 1081 |
| Hpy8I | GTNNAC | 2 cut(s) | 33, 415 |
| Hpy99I | CGWCG | 3 cut(s) | 50, 188, 569 |
| HpyAV | CCTTC | 5 cut(s) | 101, 212, 458, 971, 1054 |
| HpyCH4III | ACNGT | 5 cut(s) | 426, 637, 654, 885, 1018 |
| HpyCH4IV | ACGT | 1 cut(s) | 138 |
| HpyCH4V | TGCA | 7 cut(s) | 208, 331, 346, 448, 828, 943, 1042 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 34, 75, 1039 |
| HpyF3I | CTNAG | 2 cut(s) | 857, 1101 |
| HpySE526I | ACGT | 1 cut(s) | 138 |
| Hsp92I | GRCGYC | 2 cut(s) | 138, 609 |
| Hsp92II | CATG | 1 cut(s) | 950 |
| HspAI | GCGC | 1 cut(s) | 735 |
| Kpn2I | TCCGGA | 1 cut(s) | 494 |
| Ksp22I | TGATCA | 1 cut(s) | 960 |
| Kzo9I | GATC | 3 cut(s) | 730, 808, 960 |
| LguI | GCTCTTC | 1 cut(s) | 147 |
| LmnI | GCTCC | 5 cut(s) | 520, 523, 572, 742, 806 |
| Lsp1109I | GCAGC | 5 cut(s) | 24, 87, 315, 460, 812 |
| LweI | GCATC | 1 cut(s) | 333 |
| MaeI | CTAG | 2 cut(s) | 8, 125 |
| MaeII | ACGT | 1 cut(s) | 138 |
| MaeIII | GTNAC | 3 cut(s) | 553, 901, 1078 |
| MalI | GATC | 3 cut(s) | 732, 810, 962 |
| MbiI | CCGCTC | 1 cut(s) | 515 |
| MboI | GATC | 3 cut(s) | 730, 808, 960 |
| MboII | GAAGA | 5 cut(s) | 164, 352, 432, 713, 986 |
| MhlI | GDGCHC | 3 cut(s) | 316, 789, 803 |
| MlyI | GAGTC | 2 cut(s) | 113, 1073 |
| MmeI | TCCRAC | 3 cut(s) | 166, 285, 1000 |
| MnlI | CCTC | 9 cut(s) | 113, 208, 361, 378, 461, 563, 733, 946, 1004 |
| MroI | TCCGGA | 1 cut(s) | 494 |
| MroXI | GAANNNNTTC | 1 cut(s) | 873 |
| MseI | TTAA | 4 cut(s) | 84, 201, 645, 887 |
| MspA1I | CMGCKG | 1 cut(s) | 825 |
| MspI | CCGG | 1 cut(s) | 495 |
| MspR9I | CCNGG | 1 cut(s) | 677 |
| MssI | GTTTAAAC | 1 cut(s) | 646 |
| MvaI | CCWGG | 1 cut(s) | 677 |
| MvnI | CGCG | 1 cut(s) | 735 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 34, 75, 1039 |
| NdeII | GATC | 3 cut(s) | 730, 808, 960 |
| NlaIII | CATG | 1 cut(s) | 950 |
| NmuCI | GTSAC | 3 cut(s) | 553, 901, 1078 |
| NspI | RCATGY | 1 cut(s) | 950 |
| NspV | TTCGAA | 1 cut(s) | 253 |
| PciI | ACATGT | 1 cut(s) | 946 |
| PciSI | GCTCTTC | 1 cut(s) | 147 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 189 |
| PdmI | GAANNNNTTC | 1 cut(s) | 873 |
| PfeI | GAWTC | 2 cut(s) | 680, 869 |
| PkrI | GCNGC | 5 cut(s) | 39, 77, 330, 450, 827 |
| PleI | GAGTC | 2 cut(s) | 113, 1072 |
| PmeI | GTTTAAAC | 1 cut(s) | 646 |
| PpsI | GAGTC | 2 cut(s) | 113, 1072 |
| PscI | ACATGT | 1 cut(s) | 946 |
| Psp124BI | GAGCTC | 1 cut(s) | 789 |
| Psp6I | CCWGG | 1 cut(s) | 675 |
| PspFI | CCCAGC | 1 cut(s) | 458 |
| PspGI | CCWGG | 1 cut(s) | 675 |
| PspPI | GGNCC | 1 cut(s) | 819 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 16, 48 |
| PstI | CTGCAG | 1 cut(s) | 450 |
| PvuII | CAGCTG | 1 cut(s) | 825 |
| RsaI | GTAC | 3 cut(s) | 129, 505, 1015 |
| RsaNI | GTAC | 3 cut(s) | 128, 504, 1014 |
| SacI | GAGCTC | 1 cut(s) | 789 |
| SapI | GCTCTTC | 1 cut(s) | 147 |
| SaqAI | TTAA | 4 cut(s) | 84, 201, 645, 887 |
| SatI | GCNGC | 5 cut(s) | 38, 76, 329, 449, 826 |
| Sau3AI | GATC | 3 cut(s) | 730, 808, 960 |
| Sau96I | GGNCC | 1 cut(s) | 819 |
| SchI | GAGTC | 2 cut(s) | 113, 1073 |
| ScrFI | CCNGG | 1 cut(s) | 677 |
| SduI | GDGCHC | 3 cut(s) | 316, 789, 803 |
| SfaNI | GCATC | 1 cut(s) | 333 |
| SfcI | CTRYAG | 1 cut(s) | 446 |
| SfuI | TTCGAA | 1 cut(s) | 253 |
| SsiI | CCGC | 5 cut(s) | 21, 51, 513, 956, 981 |
| SspMI | CTAG | 2 cut(s) | 8, 125 |
| SstI | GAGCTC | 1 cut(s) | 789 |
| StyD4I | CCNGG | 1 cut(s) | 675 |
| StyI | CCWWGG | 1 cut(s) | 804 |
| TaaI | ACNGT | 5 cut(s) | 426, 637, 654, 885, 1018 |
| TaiI | ACGT | 1 cut(s) | 141 |
| TaqI | TCGA | 5 cut(s) | 192, 245, 253, 383, 564 |
| TatI | WGTACW | 1 cut(s) | 503 |
| TfiI | GAWTC | 2 cut(s) | 680, 869 |
| Tru1I | TTAA | 4 cut(s) | 84, 201, 645, 887 |
| Tru9I | TTAA | 4 cut(s) | 84, 201, 645, 887 |
| TscAI | CASTG | 5 cut(s) | 120, 594, 657, 850, 945 |
| TseFI | GTSAC | 3 cut(s) | 553, 901, 1078 |
| TseI | GCWGC | 5 cut(s) | 37, 75, 328, 448, 825 |
| Tsp45I | GTSAC | 3 cut(s) | 553, 901, 1078 |
| TspDTI | ATGAA | 2 cut(s) | 423, 882 |
| TspGWI | ACGGA | 1 cut(s) | 582 |
| TspRI | CASTG | 5 cut(s) | 120, 594, 657, 850, 945 |
| XapI | RAATTY | 4 cut(s) | 11, 247, 484, 999 |
| XceI | RCATGY | 1 cut(s) | 950 |
| XmnI | GAANNNNTTC | 1 cut(s) | 873 |
| XspI | CTAG | 2 cut(s) | 8, 125 |
| ZraI | GACGTC | 1 cut(s) | 139 |