MD06G1115800.v1.1

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000408
Physical Location & Seq
Forward (+)
25477458 .. 25481138
3681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1115800.v1.1.491

Sequence Viewer

Length: 3243 bp
ATGAAAATTGAAGAGAGTGGTATCATACAATACGATCCATTAGTTGAACTTCCATCGGGGTACGGGGGAGAGTCTTCAGGAAGTGGCGCAGAGACAACACCGCATGAACAAAATCGAGAGAATGGTGGACAACAAATGGCGCCGTCAGGTGAAGGAGTAGGGGCTGCTGCTCTAGGAGCGGCGTTAGCAAGGGATGTGAGGGAGAGCTTGGTTTCGGTAAGCAATATAGAGGCGGTGATCAATCAAATTGAAGGGAGTGGTGTGGTAAAATTACAAAATGAGCAAAATGAAGCTAAAGGTTCATGTGAAGTGCCCGCAGCCATGGCTAAGAGTGAAGTACGATTGAATGCACTAAGTGTGGAGGGAACAAGGGACTTGACTGAGACATTGGATCCGGAAGCGAATATAATGGTAGGGTGGCAAAATGTTGAAGACAGTTGGGTGGCACAAAGTAAAACTGAACATCCATCTCGTACACTTGGACTGGATGCGATGAACATGCAGGTGGGAGCAAGGAGTATGGAGGAGACCTCTGTAAGTCATGTAGAGGAGGGGCTAAAGCGAATTCAGTGCGAAGAAGTATCCGAACCTGATGCGCCATTTAGGGAATTGAAGGAGGATGTTCTTAAGGATGAGGAAGATGAGGAACTTGATGTGCAAGTGGAAGATCATCATGACAGGAAGAAGAAAGGAGCTGAGTCTGTGACAGGTGCAAGTGGAGTCAGGATGGCGATTTGTAAAGCTGATGGGTGTGCAGTCGATCTGAGTGATGCAAAGGTGTATAATAGAAGGCATACGGTGTGCGAACTTCATTCAAAGGCTCCACTTGTGATTGTGTCGGGTCTAAAGAGAAGGTTTTGCCATAAATGCAGCAGATTTCATGATCTATCAGAATTTGATGACACCAAACAAAGTTGTCGTAGGCATTTGCCCTACATTGAACGTGATGGTACATGCACTCAAGTAAAGGACGTAAGTGGTGATATAGATACGGTGATTAGTCAAATTGAATGGAGTGGTGTGGTACAATCACAAAATGATCAAAGTGAAATTAAAGATTCATGTGCAGTGGTCGAAACCACATCACCAGCAGTTGGATTGAATATATCGAGTGTGAAGGGAACAAGGGATGTAAAGGAGACATTGCAGACCTCAGCAAGTAATACAGAGGCAGGACTGGAGCAAATTCAGGGGAAAGAAGTGCCTGATTTTGAACCTCCGTACGCTGAATATAAGGAAACTGAATACATACCTTCATCGGTTACAGTTGGCTTGGATGTGCTTTTGAGAGGATTGAAGAGGTTTCTTCTTTATGATGAGGTGTCAACGGTTGTGCTAACGGGTCCTCAAGGAAGTGGGAAAACCACATTGGCAAAATACTTCTGTGCAGATGAGGAAGTTAAAAACATATTCAAGAACAACATCTTCTTTGTTCTTGTTTCAAAAAATCCAAGCTTGAGCCTTATGGTACAAACACTACATCAACAGATTGAACCCAAGGGTTTCCAGATACCTGCGTTGCAAGACGAATCAATTGCACTTCACTGGTTGCACCAATTTTTCATGGAAACAGCACTGCACCCATCAGTGTTGGTCTTGGATGATGTTTGGCCTGGATCAGAATCCCTTCTTGACAAGTTTGATGAATTCAAAACACCAAACTACAAGATTATAGTGACATCAAGGTCTGAATTTCCAACATTTGGTTGTCCATATTATCTGAAATCACTGAATGCTGAAGATGAGATGACTCTTCCTCATCAATCCCACACACACATCGAATCACTGGATGATGAAGACCTGACAAGTGTTTCTCCGCCACCCTCCGTCACATTTGATGATAAAGGCCTGATAGATCATTTTCCTGATTCCTCTGCGTCCAACTTGGGAGACAGACGCTCTCATGTTTCAACACCTATGGGGCCAATGGATCAATTTCAGGATTCCTCGGCATCAACCTTGGGAGATAAGAGCTCGTGCGTTCCAAAAGATGCAGGGACAATGTTTCCGCTTGACAACCTCGTCAAGTCTACGCAGACATATATGCAGAAGCTGGTATTCAAGGTCAAGAAAGAAAAGAAGAAAGCCATGAAAGCTGTTTCTGGTGTTGAAGATCTTCTCGGGCGAAAAAAAAAAGAATCGGCCCTTGATGTTTATATCGACAGTCGTAACCAATCAGGAACTGCCAAAGGCTGGAAGCTAATAGGTGCCCGGGAAGCATTAAGCATCAACCTCGCGACATTTGAGAAGCCCCTTCAAAAGCTCACTTTTGCAGATCTTCTTGAGGCCACCAATGGTTTCCACGATAACAGCCTTATTGGCAAAGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAAGATGGCAGTGTCGTAGCCATAAAGAAACTAATACATATCAGCGGACAGGGTGATCGCGAATTCACTGCAGAAATGGAAACAATAGGGAAGATCAAGCACCGGAACCTTGTCCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTGGTTTATGAGTACATGAAATATGGAAGCTTAGATGATGTTTTACATGAACCGAAGAAAGCTGGCATCAAGTTGAACTGGGCTGCAAGGAGGAAGATTGCCATTGGGTCTGCGAGGGGACTGGCCTTTCTTCACCACAATTGCATCCCACACATCATTCACAGGGATATGAAATCGAGCAATGTGCTGGTGGATGAAAATTTAGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTGCAATGGACACCCATTTGAGTGTGAGCACTCTAGCAGGCACCCCTGGTTATGTCCCTCCTGAATACTACCAGAGCTTTAGATGTTCCACGAAAGGTGATGTTTATAGTTATGGAGTAGTATTGCTTGAACTGCTAACGGGAAGACGTCCTGCAGATTCAGCAGATTTTGGCGACAATAATCTCGTGGGTTGGGTAAAACAACACGCTAAATTGAAAATAAGTGATGTTTTTGATCCGGAGCTCATGAAAGAGGACGCAAGCCTTGAGATTGAGCTTTTACAGCACTTGAAGGTAGCTTGTGCTTGTTTGGACGACAGGCCATGGCGGCGTCCGACAATGATCCAAGTGATGGCAATGTTCAAGGAAACCCAAGCCGGGTCTGGGATGGATTCCCAATCAACGATAGCCACGGACGACGACGGAGTTTTTGGCGGAGTTGAAATGGTAGAGATGAGCATGAAAGAAGTCCCGGAAAGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Pkinase zf-RVT

Protein Analysis

1081

Amino Acids

119.07

Weight (kDa)

5.18

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 246 - 310 6.4e-24 SBP domain
NB-ARC PF00931 432 - 564 2.8e-11 NB-ARC domain
Pkinase PF00069 767 - 1037 1.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 771 - 1036 1.1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 493
AasI GACNNNNNNGTC 2 cut(s) 1684, 2021
AatII GACGTC 1 cut(s) 2905
Acc36I ACCTGC 2 cut(s) 493, 1522
AccB1I GGYRCC 3 cut(s) 139, 2207, 2795
AccB7I CCANNNNNTGG 4 cut(s) 1094, 1703, 2193, 3106
AccBSI CCGCTC 1 cut(s) 179
AccI GTMKAC 2 cut(s) 2030, 2343
AccII CGCG 2 cut(s) 2237, 2418
AccIII TCCGGA 2 cut(s) 394, 2992
AciI CCGC 9 cut(s) 101, 179, 233, 315, 1817, 2009, 2403, 3082, 3189
AclWI GGATC 7 cut(s) 29, 386, 399, 1624, 1938, 2984, 3091
AcsI RAATTY 7 cut(s) 564, 893, 1185, 1646, 1691, 2420, 2710
AcuI CTGAAG 2 cut(s) 60, 1758
AcyI GRCGYC 3 cut(s) 140, 2902, 3085
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 8 cut(s) 62, 339, 475, 952, 1026, 1223, 1470, 2525
AfiI CCNNNNNNNGG 7 cut(s) 1094, 1703, 2193, 2480, 3106, 3132, 3138
AflII CTTAAG 1 cut(s) 626
AjnI CCWGG 2 cut(s) 1612, 2800
AjuI GAANNNNNNNTTGG 2 cut(s) 1352, 1384
Alw21I GWGCWC 3 cut(s) 1976, 2786, 3000
Alw26I GTCTC 6 cut(s) 86, 377, 521, 1133, 1884, 2731
AlwI GGATC 7 cut(s) 29, 386, 399, 1624, 1938, 2984, 3091
AlwNI CAGNNNCTG 2 cut(s) 2053, 2183
Ama87I CYCGRG 2 cut(s) 2120, 2211
Aor13HI TCCGGA 2 cut(s) 394, 2992
AoxI GGCC 8 cut(s) 1610, 1846, 1922, 2142, 2286, 2349, 2634, 3074
ApeKI GCWGC 5 cut(s) 164, 167, 317, 870, 2594
ApoI RAATTY 7 cut(s) 564, 893, 1185, 1646, 1691, 2420, 2710
ArsI GACNNNNNNTTYG 2 cut(s) 3167, 3199
Asp700I GAANNNNTTC 2 cut(s) 1626, 2115
AspLEI GCGC 3 cut(s) 89, 142, 598
AspS9I GGNCC 4 cut(s) 1343, 1922, 2143, 2350
AsuC2I CCSGG 4 cut(s) 2212, 2213, 3133, 3227
AsuHPI GGTGA 9 cut(s) 161, 247, 992, 1006, 1077, 2348, 2423, 2636, 2864
AvaI CYCGRG 2 cut(s) 2120, 2211
AvaII GGWCC 1 cut(s) 1343
BaeGI GKGCMC 2 cut(s) 315, 2212
BaeI ACNNNNGTAYC 2 cut(s) 1502, 1535
BamHI GGATCC 1 cut(s) 391
BanI GGYRCC 3 cut(s) 139, 2207, 2795
BanII GRGCYC 2 cut(s) 1976, 3000
BauI CACGAG 2 cut(s) 1975, 2939
BbsI GAAGAC 4 cut(s) 66, 438, 1803, 2905
Bbv12I GWGCWC 3 cut(s) 1976, 2786, 3000
BbvCI CCTCAGC 1 cut(s) 1153
BbvI GCAGC 5 cut(s) 151, 154, 329, 882, 2581
BccI CCATC 9 cut(s) 61, 475, 721, 740, 941, 1591, 2357, 3100, 3136
BceAI ACGGC 2 cut(s) 127, 2522
BciT130I CCWGG 2 cut(s) 1614, 2802
BciVI GTATCC 2 cut(s) 592, 2477
BclI TGATCA 2 cut(s) 237, 1039
BcnI CCSGG 4 cut(s) 2212, 2213, 3133, 3227
BcoDI GTCTC 6 cut(s) 86, 377, 521, 1133, 1884, 2731
BfaI CTAG 2 cut(s) 173, 2789
BfmI CTRYAG 2 cut(s) 2427, 2907
BfoI RGCGCY 1 cut(s) 143
BfrI CTTAAG 1 cut(s) 626
BfuAI ACCTGC 2 cut(s) 493, 1522
BfuI GTATCC 2 cut(s) 592, 2477
BglI GCCNNNNNGGC 2 cut(s) 2319, 3082
BglII AGATCT 2 cut(s) 2113, 2275
BisI GCNGC 7 cut(s) 165, 168, 180, 318, 871, 2595, 3083
BlsI GCNGC 7 cut(s) 166, 169, 181, 319, 872, 2596, 3084
Bme1390I CCNGG 6 cut(s) 1614, 2212, 2213, 2802, 3133, 3227
Bme18I GGWCC 1 cut(s) 1343
BmeT110I CYCGRG 2 cut(s) 2120, 2211
BmgT120I GGNCC 4 cut(s) 1343, 1922, 2143, 2350
BmiI GGNNCC 8 cut(s) 141, 393, 822, 1344, 1923, 2209, 2465, 2797
BmrFI CCNGG 6 cut(s) 1614, 2212, 2213, 2802, 3133, 3227
BmrI ACTGGG 1 cut(s) 2599
BmsI GCATC 8 cut(s) 478, 583, 760, 1961, 1981, 2235, 2586, 2664
BmuI ACTGGG 1 cut(s) 2599
BpiI GAAGAC 4 cut(s) 66, 438, 1803, 2905
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 1 cut(s) 1199
Bpu10I CCTNAGC 1 cut(s) 1153
BpuEI CTTGAG 5 cut(s) 945, 1332, 1477, 2303, 3041
BpuMI CCSGG 4 cut(s) 2212, 2213, 3133, 3227
BsaBI GATNNNNATC 1 cut(s) 1621
BsaHI GRCGYC 3 cut(s) 140, 2902, 3085
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 9 cut(s) 321, 1497, 1947, 1959, 2211, 2479, 2800, 3077, 3165
BsaWI WCCGGW 3 cut(s) 394, 2460, 2992
Bsc4I CCNNNNNNNGG 7 cut(s) 1094, 1703, 2193, 2480, 3106, 3132, 3138
Bse1I ACTGG 6 cut(s) 489, 1182, 1550, 1791, 2594, 2637
Bse3DI GCAATG 4 cut(s) 1142, 2698, 2766, 3117
Bse8I GATNNNNATC 1 cut(s) 1621
BseAI TCCGGA 2 cut(s) 394, 2992
BseBI CCWGG 2 cut(s) 1614, 2802
BseDI CCNNGG 9 cut(s) 321, 1497, 1947, 1959, 2211, 2479, 2800, 3077, 3165
BseJI GATNNNNATC 1 cut(s) 1621
BseLI CCNNNNNNNGG 7 cut(s) 1094, 1703, 2193, 2480, 3106, 3132, 3138
BseMI GCAATG 4 cut(s) 1142, 2698, 2766, 3117
BseMII CTCAG 4 cut(s) 372, 687, 755, 1167
BseNI ACTGG 6 cut(s) 489, 1182, 1550, 1791, 2594, 2637
BseRI GAGGAG 2 cut(s) 539, 563
BseSI GKGCMC 2 cut(s) 315, 2212
BseXI GCAGC 5 cut(s) 151, 154, 329, 882, 2581
BsgI GTGCAG 4 cut(s) 774, 1086, 1407, 1562
Bsh1236I CGCG 2 cut(s) 2237, 2418
BshFI GGCC 8 cut(s) 1612, 1848, 1924, 2144, 2288, 2351, 2636, 3076
BshNI GGYRCC 3 cut(s) 139, 2207, 2795
BsiHKAI GWGCWC 3 cut(s) 1976, 2786, 3000
BsiHKCI CYCGRG 2 cut(s) 2120, 2211
BsiSI CCGG 6 cut(s) 395, 2212, 2461, 2993, 3132, 3227
BsiWI CGTACG 1 cut(s) 1221
BslFI GGGAC 6 cut(s) 386, 2011, 2456, 2643, 2795, 3209
BslI CCNNNNNNNGG 7 cut(s) 1094, 1703, 2193, 2480, 3106, 3132, 3138
BsmAI GTCTC 6 cut(s) 86, 377, 521, 1133, 1884, 2731
BsmFI GGGAC 6 cut(s) 386, 2011, 2456, 2643, 2795, 3209
BsmI GAATGC 2 cut(s) 352, 1738
BsnI GGCC 8 cut(s) 1612, 1848, 1924, 2144, 2288, 2351, 2636, 3076
Bso31I GGTCTC 1 cut(s) 521
BsoBI CYCGRG 2 cut(s) 2120, 2211
Bsp1286I GDGCHC 5 cut(s) 315, 1976, 2212, 2786, 3000
Bsp13I TCCGGA 2 cut(s) 394, 2992
Bsp19I CCATGG 2 cut(s) 321, 3077
Bsp68I TCGCGA 2 cut(s) 2237, 2418
BspACI CCGC 9 cut(s) 101, 179, 233, 315, 1817, 2009, 2403, 3082, 3189
BspANI GGCC 8 cut(s) 1612, 1848, 1924, 2144, 2288, 2351, 2636, 3076
BspCNI CTCAG 4 cut(s) 373, 688, 756, 1166
BspEI TCCGGA 2 cut(s) 394, 2992
BspFNI CGCG 2 cut(s) 2237, 2418
BspHI TCATGA 3 cut(s) 673, 880, 3000
BspLI GGNNCC 8 cut(s) 141, 393, 822, 1344, 1923, 2209, 2465, 2797
BspMAI CTGCAG 2 cut(s) 2431, 2911
BspMI ACCTGC 2 cut(s) 493, 1522
BspPI GGATC 7 cut(s) 29, 386, 399, 1624, 1938, 2984, 3091
BspT107I GGYRCC 3 cut(s) 139, 2207, 2795
BspTI CTTAAG 1 cut(s) 626
BspTNI GGTCTC 1 cut(s) 521
BsrBI CCGCTC 1 cut(s) 179
BsrDI GCAATG 4 cut(s) 1142, 2698, 2766, 3117
BsrI ACTGG 6 cut(s) 489, 1182, 1550, 1791, 2594, 2637
BssECI CCNNGG 9 cut(s) 321, 1497, 1947, 1959, 2211, 2479, 2800, 3077, 3165
BssNAI GTATAC 1 cut(s) 2344
BssNI GRCGYC 3 cut(s) 140, 2902, 3085
BssSI CACGAG 2 cut(s) 1975, 2939
BssT1I CCWWGG 5 cut(s) 321, 1497, 1959, 2479, 3077
Bst1107I GTATAC 1 cut(s) 2344
Bst2BI CACGAG 2 cut(s) 1975, 2939
Bst2UI CCWGG 2 cut(s) 1614, 2802
Bst4CI ACNGT 6 cut(s) 437, 799, 994, 1267, 1330, 2165
Bst6I CTCTTC 3 cut(s) 6, 1292, 1758
BstACI GRCGYC 3 cut(s) 140, 2902, 3085
BstAFI CTTAAG 1 cut(s) 626
BstC8I GCNNGC 5 cut(s) 315, 2575, 2794, 3016, 3236
BstDEI CTNAG 7 cut(s) 327, 353, 381, 696, 764, 1153, 2542
BstDSI CCRYGG 3 cut(s) 321, 3077, 3165
BstFNI CGCG 2 cut(s) 2237, 2418
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 3 cut(s) 89, 142, 598
BstMAI GTCTC 6 cut(s) 86, 377, 521, 1133, 1884, 2731
BstNI CCWGG 2 cut(s) 1614, 2802
BstNSI RCATGY 2 cut(s) 502, 957
BstSCI CCNGG 6 cut(s) 1612, 2210, 2211, 2800, 3131, 3225
BstSFI CTRYAG 2 cut(s) 2427, 2907
BstSLI GKGCMC 2 cut(s) 315, 2212
BstUI CGCG 2 cut(s) 2237, 2418
BstV1I GCAGC 5 cut(s) 151, 154, 329, 882, 2581
BstV2I GAAGAC 4 cut(s) 66, 438, 1803, 2905
BstX2I RGATCY 3 cut(s) 391, 2113, 2275
BstYI RGATCY 3 cut(s) 391, 2113, 2275
BstZ17I GTATAC 1 cut(s) 2344
BsuI GTATCC 2 cut(s) 592, 2477
BsuRI GGCC 8 cut(s) 1612, 1848, 1924, 2144, 2288, 2351, 2636, 3076
BtgI CCRYGG 3 cut(s) 321, 3077, 3165
BtgZI GCGATG 1 cut(s) 506
BtsI GCAGTG 4 cut(s) 1074, 1574, 2374, 2424
BtsIMutI CAGTG 9 cut(s) 575, 1074, 1543, 1574, 1593, 1727, 1784, 2374, 2424
BtuMI TCGCGA 2 cut(s) 2237, 2418
BveI ACCTGC 2 cut(s) 493, 1522
Cac8I GCNNGC 5 cut(s) 315, 2575, 2794, 3016, 3236
CaiI CAGNNNCTG 2 cut(s) 2053, 2183
CciI TCATGA 3 cut(s) 673, 880, 3000
CfoI GCGC 3 cut(s) 89, 142, 598
Cfr13I GGNCC 4 cut(s) 1343, 1922, 2143, 2350
Cfr9I CCCGGG 1 cut(s) 2211
CseI GACGC 4 cut(s) 1866, 1905, 3020, 3074
Csp6I GTAC 8 cut(s) 61, 338, 474, 951, 1025, 1222, 1469, 2524
CviQI GTAC 8 cut(s) 61, 338, 474, 951, 1025, 1222, 1469, 2524
DdeI CTNAG 7 cut(s) 327, 353, 381, 696, 764, 1153, 2542
DinI GGCGCC 1 cut(s) 141
DraIII CACNNNGTG 1 cut(s) 356
DrdI GACNNNNNNGTC 2 cut(s) 1684, 2021
DseDI GACNNNNNNGTC 2 cut(s) 1684, 2021
Eam1104I CTCTTC 3 cut(s) 6, 1292, 1758
EarI CTCTTC 3 cut(s) 6, 1292, 1758
EciI GGCGGA 2 cut(s) 1806, 3204
Ecl136II GAGCTC 2 cut(s) 1974, 2998
Eco130I CCWWGG 5 cut(s) 321, 1497, 1959, 2479, 3077
Eco147I AGGCCT 1 cut(s) 1848
Eco24I GRGCYC 2 cut(s) 1976, 3000
Eco31I GGTCTC 1 cut(s) 521
Eco47I GGWCC 1 cut(s) 1343
Eco53kI GAGCTC 2 cut(s) 1974, 2998
Eco57I CTGAAG 2 cut(s) 60, 1758
Eco88I CYCGRG 2 cut(s) 2120, 2211
EcoICRI GAGCTC 2 cut(s) 1974, 2998
EcoO109I RGGNCCY 1 cut(s) 1343
EcoRI GAATTC 3 cut(s) 564, 1646, 2420
EcoRII CCWGG 2 cut(s) 1612, 2800
EcoT14I CCWWGG 5 cut(s) 321, 1497, 1959, 2479, 3077
EcoT38I GRGCYC 2 cut(s) 1976, 3000
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
ErhI CCWWGG 5 cut(s) 321, 1497, 1959, 2479, 3077
FalI AAGNNNNNCTT 2 cut(s) 2493, 2525
FaqI GGGAC 6 cut(s) 386, 2011, 2456, 2643, 2795, 3209
FauI CCCGC 1 cut(s) 322
FbaI TGATCA 2 cut(s) 237, 1039
FblI GTMKAC 2 cut(s) 2030, 2343
Fnu4HI GCNGC 7 cut(s) 165, 168, 180, 318, 871, 2595, 3083
FriOI GRGCYC 2 cut(s) 1976, 3000
Fsp4HI GCNGC 7 cut(s) 165, 168, 180, 318, 871, 2595, 3083
FspBI CTAG 2 cut(s) 173, 2789
GlaI GCGC 3 cut(s) 88, 141, 597
GluI GCNGC 7 cut(s) 165, 168, 180, 318, 871, 2595, 3083
GsuI CTGGAG 1 cut(s) 1199
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 8 cut(s) 1612, 1848, 1924, 2144, 2288, 2351, 2636, 3076
HapII CCGG 6 cut(s) 395, 2212, 2461, 2993, 3132, 3227
HgaI GACGC 4 cut(s) 1866, 1905, 3020, 3074
HhaI GCGC 3 cut(s) 89, 142, 598
Hin1I GRCGYC 3 cut(s) 140, 2902, 3085
Hin6I GCGC 3 cut(s) 87, 140, 596
HinP1I GCGC 3 cut(s) 87, 140, 596
HincII GTYRAC 1 cut(s) 1326
HindII GTYRAC 1 cut(s) 1326
HindIII AAGCTT 2 cut(s) 1453, 2539
HpaII CCGG 6 cut(s) 395, 2212, 2461, 2993, 3132, 3227
HphI GGTGA 9 cut(s) 161, 247, 992, 1006, 1077, 2348, 2423, 2636, 2864
Hpy166II GTNNAC 5 cut(s) 128, 476, 1326, 2031, 2344
Hpy188I TCNGA 8 cut(s) 586, 765, 892, 1621, 1690, 1722, 2731, 3090
Hpy8I GTNNAC 5 cut(s) 128, 476, 1326, 2031, 2344
Hpy99I CGWCG 2 cut(s) 3176, 3179
HpyCH4III ACNGT 6 cut(s) 437, 799, 994, 1267, 1330, 2165
HpyCH4IV ACGT 3 cut(s) 943, 972, 2902
HpyF3I CTNAG 7 cut(s) 327, 353, 381, 696, 764, 1153, 2542
HpySE526I ACGT 3 cut(s) 943, 972, 2902
Hsp92I GRCGYC 3 cut(s) 140, 2902, 3085
HspAI GCGC 3 cut(s) 87, 140, 596
KasI GGCGCC 1 cut(s) 139
Kpn2I TCCGGA 2 cut(s) 394, 2992
Ksp22I TGATCA 2 cut(s) 237, 1039
LmnI GCTCC 6 cut(s) 176, 509, 692, 826, 1180, 2995
Lsp1109I GCAGC 5 cut(s) 151, 154, 329, 882, 2581
LweI GCATC 8 cut(s) 478, 583, 760, 1961, 1981, 2235, 2586, 2664
MaeI CTAG 2 cut(s) 173, 2789
MaeII ACGT 3 cut(s) 943, 972, 2902
MaeIII GTNAC 5 cut(s) 703, 1261, 1675, 1828, 2168
MbiI CCGCTC 1 cut(s) 179
MfeI CAATTG 3 cut(s) 1533, 2354, 2650
MflI RGATCY 3 cut(s) 391, 2113, 2275
MhlI GDGCHC 5 cut(s) 315, 1976, 2212, 2786, 3000
Mly113I GGCGCC 1 cut(s) 140
MlyI GAGTC 5 cut(s) 80, 707, 729, 1744, 2733
MmeI TCCRAC 4 cut(s) 1075, 1721, 1905, 3113
MroI TCCGGA 2 cut(s) 394, 2992
MroXI GAANNNNTTC 2 cut(s) 1626, 2115
MseI TTAA 4 cut(s) 627, 1053, 1401, 2222
MslI CAYNNNNRTG 2 cut(s) 503, 2775
MspA1I CMGCKG 1 cut(s) 2403
MspCI CTTAAG 1 cut(s) 626
MspI CCGG 6 cut(s) 395, 2212, 2461, 2993, 3132, 3227
MspR9I CCNGG 6 cut(s) 1614, 2212, 2213, 2802, 3133, 3227
MunI CAATTG 3 cut(s) 1533, 2354, 2650
Mva1269I GAATGC 2 cut(s) 352, 1738
MvaI CCWGG 2 cut(s) 1614, 2802
MvnI CGCG 2 cut(s) 2237, 2418
NarI GGCGCC 1 cut(s) 140
NciI CCSGG 4 cut(s) 2212, 2213, 3133, 3227
NcoI CCATGG 2 cut(s) 321, 3077
NlaIV GGNNCC 8 cut(s) 141, 393, 822, 1344, 1923, 2209, 2465, 2797
NmeAIII GCCGAG 1 cut(s) 1928
NmuCI GTSAC 3 cut(s) 703, 1675, 1828
NruI TCGCGA 2 cut(s) 2237, 2418
NspI RCATGY 2 cut(s) 502, 957
PagI TCATGA 3 cut(s) 673, 880, 3000
PaqCI CACCTGC 1 cut(s) 493
PceI AGGCCT 1 cut(s) 1848
PctI GAATGC 2 cut(s) 352, 1738
PdmI GAANNNNTTC 2 cut(s) 1626, 2115
PfeI GAWTC 9 cut(s) 1058, 1529, 1622, 1781, 1868, 1943, 2138, 2912, 3146
Pfl23II CGTACG 1 cut(s) 1221
PflMI CCANNNNNTGG 4 cut(s) 1094, 1703, 2193, 3106
PfoI TCCNGGA 1 cut(s) 3225
PkrI GCNGC 7 cut(s) 166, 169, 181, 319, 872, 2596, 3084
PleI GAGTC 5 cut(s) 79, 706, 728, 1744, 2732
PluTI GGCGCC 1 cut(s) 143
PpsI GAGTC 5 cut(s) 79, 706, 728, 1744, 2732
PpuMI RGGWCCY 1 cut(s) 1343
Psp124BI GAGCTC 2 cut(s) 1976, 3000
Psp5II RGGWCCY 1 cut(s) 1343
Psp6I CCWGG 2 cut(s) 1612, 2800
PspGI CCWGG 2 cut(s) 1612, 2800
PspLI CGTACG 1 cut(s) 1221
PspN4I GGNNCC 8 cut(s) 141, 393, 822, 1344, 1923, 2209, 2465, 2797
PspPI GGNCC 4 cut(s) 1343, 1922, 2143, 2350
PspPPI RGGWCCY 1 cut(s) 1343
PstI CTGCAG 2 cut(s) 2431, 2911
PstNI CAGNNNCTG 2 cut(s) 2053, 2183
PsuI RGATCY 3 cut(s) 391, 2113, 2275
RruI TCGCGA 2 cut(s) 2237, 2418
RsaI GTAC 8 cut(s) 62, 339, 475, 952, 1026, 1223, 1470, 2525
RsaNI GTAC 8 cut(s) 61, 338, 474, 951, 1025, 1222, 1469, 2524
RseI CAYNNNNRTG 2 cut(s) 503, 2775
SacI GAGCTC 2 cut(s) 1976, 3000
SaqAI TTAA 4 cut(s) 627, 1053, 1401, 2222
SatI GCNGC 7 cut(s) 165, 168, 180, 318, 871, 2595, 3083
Sau96I GGNCC 4 cut(s) 1343, 1922, 2143, 2350
SchI GAGTC 5 cut(s) 80, 707, 729, 1744, 2733
ScrFI CCNGG 6 cut(s) 1614, 2212, 2213, 2802, 3133, 3227
SduI GDGCHC 5 cut(s) 315, 1976, 2212, 2786, 3000
SfaNI GCATC 8 cut(s) 478, 583, 760, 1961, 1981, 2235, 2586, 2664
SfcI CTRYAG 2 cut(s) 2427, 2907
SfoI GGCGCC 1 cut(s) 141
SinI GGWCC 1 cut(s) 1343
SmaI CCCGGG 1 cut(s) 2213
SmiMI CAYNNNNRTG 2 cut(s) 503, 2775
SmlI CTYRAG 6 cut(s) 626, 960, 1347, 1456, 2282, 3020
SmoI CTYRAG 6 cut(s) 626, 960, 1347, 1456, 2282, 3020
SseBI AGGCCT 1 cut(s) 1848
SsiI CCGC 9 cut(s) 101, 179, 233, 315, 1817, 2009, 2403, 3082, 3189
SspDI GGCGCC 1 cut(s) 139
SspMI CTAG 2 cut(s) 173, 2789
SstI GAGCTC 2 cut(s) 1976, 3000
StuI AGGCCT 1 cut(s) 1848
StyD4I CCNGG 6 cut(s) 1612, 2210, 2211, 2800, 3131, 3225
StyI CCWWGG 5 cut(s) 321, 1497, 1959, 2479, 3077
TaaI ACNGT 6 cut(s) 437, 799, 994, 1267, 1330, 2165
TaiI ACGT 3 cut(s) 946, 975, 2905
TaqI TCGA 7 cut(s) 115, 759, 1074, 1109, 1779, 2160, 2687
TatI WGTACW 1 cut(s) 2523
TauI GCSGC 2 cut(s) 182, 3085
TfiI GAWTC 9 cut(s) 1058, 1529, 1622, 1781, 1868, 1943, 2138, 2912, 3146
Tru1I TTAA 4 cut(s) 627, 1053, 1401, 2222
Tru9I TTAA 4 cut(s) 627, 1053, 1401, 2222
TscAI CASTG 9 cut(s) 575, 1074, 1550, 1581, 1593, 1734, 1791, 2374, 2431
TseFI GTSAC 3 cut(s) 703, 1675, 1828
TseI GCWGC 5 cut(s) 164, 167, 317, 870, 2594
Tsp45I GTSAC 3 cut(s) 703, 1675, 1828
TspGWI ACGGA 4 cut(s) 1209, 1816, 3182, 3192
TspMI CCCGGG 1 cut(s) 2211
TspRI CASTG 9 cut(s) 575, 1074, 1550, 1581, 1593, 1734, 1791, 2374, 2431
Van91I CCANNNNNTGG 4 cut(s) 1094, 1703, 2193, 3106
Vha464I CTTAAG 1 cut(s) 626
VpaK11BI GGWCC 1 cut(s) 1343
XapI RAATTY 7 cut(s) 564, 893, 1185, 1646, 1691, 2420, 2710
XceI RCATGY 2 cut(s) 502, 957
XcmI CCANNNNNNNNNTGG 2 cut(s) 2360, 3134
XmaI CCCGGG 1 cut(s) 2211
XmiI GTMKAC 2 cut(s) 2030, 2343
XmnI GAANNNNTTC 2 cut(s) 1626, 2115
XspI CTAG 2 cut(s) 173, 2789
ZraI GACGTC 1 cut(s) 2903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.