Rroxscaffold_3G00267940
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000408
Physical Location & Seq
Reverse (-)
61102112 .. 61113817
11706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267940.1

Sequence Viewer

Length: 969 bp
ATGCCGGCCAAGCTGATCGGAGGACCGCTGCATAATCAGCTCTTTGATGGTGTTAAGACAGCAACAGGCAGGAGTACAAAGTTCACCTCCCTCCTCGAAGATCTCAAATTCACGCTAGTCTCTCTGCAACCACGAATCAATCGACAGAGAGGAGATCAGCACGATGAGGAAATTCATCATCTCCTTCCAAATGAGGAAATACAAGATCTTGAGCGACAGATGGAGGAGGGAGTGAAGCTGGTTGCATGCCAAGCTATCAAGTGTTGGAATATGGAACTACCACTGCATGAAGAGTCTCTCAGAAAAATGTTATACATACTCAAGCTGCAGGAAGAAAGAGATATAATGGAGCTCTTGCTTGTTGCTAGGACAATACGCGACAGACAGAATGTTTTGGAAAGAAGACTGTGTGGTTTACTCAAGGTGCAGCAGGAGAAAACCGGTGGCAATGGTCACGTCGACAGCGGAGGAGAAGGGGCTGGTCGGGATCTGGAGCATGTATTTTGCCGTCTACTTGAATCCCTTGTTTTGGAAGTTTATGTACAATTGAATGGGAAAACGAGGTTTAGTCCCGTCTTGGCAGATCTGAAATCAACGGTTAGGTCCTTCCAACCACTCTTGGAAGAAATATCAGATTATAACAAGTTATTGCATCTACCAAAGGAGGAACTAGACAATTTGAGGAAGCAACTGGAGAAAGGTATCGAGCTGGTCGACAAGTGCTCCAAGGTTCCTCAAGGGGCGAGCTACAAAAGGTATGAATACGCCGACGAACTACTTGAGTTGGATGACTATCTTCAGAGATTGTTGTGTATGATGAGGGAGCAGGTAGCAAAGGATTTGAAGGAGACCTTGGTTTCTTTAGATAATATTGATCCTCTTAAGCATGCCTTGGAGAGAATTACAATGGAAAGAACAATTACAATGATGACATTAACAATCGATTGGTCCGTAATCCATGCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

37.19

Weight (kDa)

5.59

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 12 - 111 1.4e-07 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 175 - 289 3.8e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
Acc36I ACCTGC 1 cut(s) 817
AccI GTMKAC 3 cut(s) 459, 511, 714
AccII CGCG 1 cut(s) 378
AciI CCGC 2 cut(s) 26, 465
AclWI GGATC 2 cut(s) 495, 869
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 2 cut(s) 107, 171
AcuI CTGAAG 1 cut(s) 782
AfaI GTAC 2 cut(s) 76, 543
AfiI CCNNNNNNNGG 1 cut(s) 529
AflII CTTAAG 1 cut(s) 881
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 3 cut(s) 518, 550, 844
AjiI CACGTC 1 cut(s) 457
AjuI GAANNNNNNNTTGG 2 cut(s) 836, 868
AluBI AGCT 8 cut(s) 13, 40, 238, 254, 325, 352, 709, 747
AluI AGCT 8 cut(s) 13, 40, 238, 254, 325, 352, 709, 747
Alw21I GWGCWC 2 cut(s) 354, 725
Alw26I GTCTC 3 cut(s) 124, 300, 842
AlwI GGATC 2 cut(s) 495, 869
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 3 cut(s) 28, 325, 427
ApoI RAATTY 2 cut(s) 107, 171
AsiGI ACCGGT 1 cut(s) 440
AspS9I GGNCC 3 cut(s) 23, 603, 948
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 3 cut(s) 23, 603, 948
BanII GRGCYC 1 cut(s) 354
BarI GAAGNNNNNNTAC 2 cut(s) 525, 557
BbsI GAAGAC 1 cut(s) 409
Bbv12I GWGCWC 2 cut(s) 354, 725
BbvI GCAGC 3 cut(s) 15, 312, 439
BccI CCATC 2 cut(s) 41, 214
BceAI ACGGC 1 cut(s) 492
BcoDI GTCTC 3 cut(s) 124, 300, 842
BfaI CTAG 3 cut(s) 116, 366, 671
BfmI CTRYAG 1 cut(s) 326
BfrI CTTAAG 1 cut(s) 881
BfuAI ACCTGC 1 cut(s) 817
BglII AGATCT 3 cut(s) 100, 205, 583
BisI GCNGC 3 cut(s) 29, 326, 428
BlsI GCNGC 3 cut(s) 30, 327, 429
Bme18I GGWCC 3 cut(s) 23, 603, 948
BmgBI CACGTC 1 cut(s) 457
BmgT120I GGNCC 3 cut(s) 23, 603, 948
BmiI GGNNCC 1 cut(s) 732
BmsI GCATC 1 cut(s) 661
BpiI GAAGAC 1 cut(s) 409
BpmI CTGGAG 2 cut(s) 512, 713
BpuEI CTTGAG 5 cut(s) 230, 305, 404, 720, 800
Bsa29I ATCGAT 1 cut(s) 942
BsaBI GATNNNNATC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 842
BsaJI CCNNGG 3 cut(s) 726, 852, 891
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse118I RCCGGY 2 cut(s) 4, 440
Bse1I ACTGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 454
Bse8I GATNNNNATC 1 cut(s) 792
BseCI ATCGAT 1 cut(s) 942
BseDI CCNNGG 3 cut(s) 726, 852, 891
BseGI GGATG 1 cut(s) 793
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMI GCAATG 1 cut(s) 454
BseMII CTCAG 1 cut(s) 313
BseNI ACTGG 1 cut(s) 696
BseRI GAGGAG 4 cut(s) 83, 165, 239, 483
BseXI GCAGC 3 cut(s) 15, 312, 439
BsgI GTGCAG 1 cut(s) 446
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 8
BshTI ACCGGT 1 cut(s) 440
BshVI ATCGAT 1 cut(s) 942
BsiHKAI GWGCWC 2 cut(s) 354, 725
BsiSI CCGG 2 cut(s) 5, 441
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 3 cut(s) 124, 300, 842
BsmFI GGGAC 1 cut(s) 555
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 842
Bsp1286I GDGCHC 2 cut(s) 354, 725
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
BspACI CCGC 2 cut(s) 26, 465
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 312
BspDI ATCGAT 1 cut(s) 942
BspFNI CGCG 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 732
BspMAI CTGCAG 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 817
BspPI GGATC 2 cut(s) 495, 869
BspTI CTTAAG 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 842
BsrDI GCAATG 1 cut(s) 454
BsrFI RCCGGY 2 cut(s) 4, 440
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 1 cut(s) 696
BssAI RCCGGY 2 cut(s) 4, 440
BssECI CCNNGG 3 cut(s) 726, 852, 891
BssMI GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
BssT1I CCWWGG 3 cut(s) 726, 852, 891
Bst4CI ACNGT 2 cut(s) 408, 598
Bst6I CTCTTC 1 cut(s) 285
BstAFI CTTAAG 1 cut(s) 881
BstAUI TGTACA 1 cut(s) 541
BstC8I GCNNGC 4 cut(s) 6, 247, 745, 888
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 793
BstFNI CGCG 1 cut(s) 378
BstKTI GATC 7 cut(s) 18, 103, 157, 208, 490, 586, 877
BstMAI GTCTC 3 cut(s) 124, 300, 842
BstMBI GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
BstMWI GCNNNNNNNGC 3 cut(s) 10, 37, 251
BstNSI RCATGY 3 cut(s) 249, 500, 890
BstSFI CTRYAG 1 cut(s) 326
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 3 cut(s) 15, 312, 439
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 4 cut(s) 100, 205, 487, 583
BstYI RGATCY 4 cut(s) 100, 205, 487, 583
Bsu15I ATCGAT 1 cut(s) 942
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 942
BtrI CACGTC 1 cut(s) 457
BtsCI GGATG 1 cut(s) 793
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 281
BveI ACCTGC 1 cut(s) 817
Cac8I GCNNGC 4 cut(s) 6, 247, 745, 888
Cfr10I RCCGGY 2 cut(s) 4, 440
Cfr13I GGNCC 3 cut(s) 23, 603, 948
ClaI ATCGAT 1 cut(s) 942
Csp6I GTAC 2 cut(s) 75, 542
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 5 cut(s) 246, 287, 497, 887, 959
CviQI GTAC 2 cut(s) 75, 542
DdeI CTNAG 1 cut(s) 299
DpnI GATC 7 cut(s) 17, 102, 156, 207, 489, 585, 876
DpnII GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 3 cut(s) 726, 852, 891
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 842
Eco47I GGWCC 3 cut(s) 23, 603, 948
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 782
EcoICRI GAGCTC 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 603
EcoT14I CCWWGG 3 cut(s) 726, 852, 891
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 3 cut(s) 726, 852, 891
FaeI CATG 5 cut(s) 249, 290, 500, 890, 962
FalI AAGNNNNNCTT 4 cut(s) 836, 868, 875, 907
FaqI GGGAC 1 cut(s) 555
FatI CATG 5 cut(s) 245, 286, 496, 886, 958
FblI GTMKAC 3 cut(s) 459, 511, 714
Fnu4HI GCNGC 3 cut(s) 29, 326, 428
FokI GGATG 1 cut(s) 800
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 3 cut(s) 29, 326, 428
FspBI CTAG 3 cut(s) 116, 366, 671
GluI GCNGC 3 cut(s) 29, 326, 428
GsuI CTGGAG 2 cut(s) 512, 713
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 5, 441
Hin1II CATG 5 cut(s) 249, 290, 500, 890, 962
HincII GTYRAC 2 cut(s) 460, 715
HindII GTYRAC 2 cut(s) 460, 715
HinfI GANTC 3 cut(s) 135, 293, 518
HpaII CCGG 2 cut(s) 5, 441
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 5 cut(s) 84, 416, 460, 512, 715
Hpy188I TCNGA 5 cut(s) 20, 302, 588, 634, 801
Hpy188III TCNNGA 3 cut(s) 209, 485, 491
Hpy8I GTNNAC 5 cut(s) 84, 416, 460, 512, 715
Hpy99I CGWCG 2 cut(s) 461, 773
HpyAV CCTTC 4 cut(s) 194, 467, 616, 838
HpyCH4III ACNGT 2 cut(s) 408, 598
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 7 cut(s) 31, 127, 245, 286, 328, 427, 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 37, 251
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 5 cut(s) 249, 290, 500, 890, 962
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
LmnI GCTCC 4 cut(s) 349, 493, 728, 823
Lsp1109I GCAGC 3 cut(s) 15, 312, 439
LweI GCATC 1 cut(s) 661
MaeI CTAG 3 cut(s) 116, 366, 671
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 452
MalI GATC 7 cut(s) 17, 102, 156, 207, 489, 585, 876
MboI GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
MboII GAAGA 6 cut(s) 110, 302, 344, 414, 635, 788
MfeI CAATTG 1 cut(s) 545
MflI RGATCY 4 cut(s) 100, 205, 487, 583
MhlI GDGCHC 2 cut(s) 354, 725
MluCI AATT 6 cut(s) 107, 171, 545, 676, 900, 918
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 3 cut(s) 245, 634, 765
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 3 cut(s) 54, 882, 935
MspA1I CMGCKG 2 cut(s) 28, 465
MspCI CTTAAG 1 cut(s) 881
MspI CCGG 2 cut(s) 5, 441
MunI CAATTG 1 cut(s) 545
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 3 cut(s) 10, 37, 251
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 249, 290, 500, 890, 962
NlaIV GGNNCC 1 cut(s) 732
NmuCI GTSAC 1 cut(s) 452
NspI RCATGY 3 cut(s) 249, 500, 890
PaeI GCATGC 2 cut(s) 249, 890
PcsI WCGNNNNNNNCGW 3 cut(s) 139, 711, 948
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 2 cut(s) 135, 518
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 3 cut(s) 30, 327, 429
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PpuMI RGGWCCY 1 cut(s) 603
PsiI TTATAA 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 354
Psp5II RGGWCCY 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 732
PspPI GGNCC 3 cut(s) 23, 603, 948
PspPPI RGGWCCY 1 cut(s) 603
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 4 cut(s) 100, 205, 487, 583
RsaI GTAC 2 cut(s) 76, 543
RsaNI GTAC 2 cut(s) 75, 542
SacI GAGCTC 1 cut(s) 354
SalI GTCGAC 2 cut(s) 458, 713
SaqAI TTAA 3 cut(s) 54, 882, 935
SatI GCNGC 3 cut(s) 29, 326, 428
Sau3AI GATC 7 cut(s) 15, 100, 154, 205, 487, 583, 874
Sau96I GGNCC 3 cut(s) 23, 603, 948
SchI GAGTC 1 cut(s) 302
SduI GDGCHC 2 cut(s) 354, 725
SfaNI GCATC 1 cut(s) 661
SfcI CTRYAG 1 cut(s) 326
SinI GGWCC 3 cut(s) 23, 603, 948
SmlI CTYRAG 6 cut(s) 209, 320, 419, 735, 779, 881
SmoI CTYRAG 6 cut(s) 209, 320, 419, 735, 779, 881
SphI GCATGC 2 cut(s) 249, 890
Sse9I AATT 6 cut(s) 107, 171, 545, 676, 900, 918
SsiI CCGC 2 cut(s) 26, 465
SspI AATATT 1 cut(s) 871
SspMI CTAG 3 cut(s) 116, 366, 671
SstI GAGCTC 1 cut(s) 354
StyI CCWWGG 3 cut(s) 726, 852, 891
TaaI ACNGT 2 cut(s) 408, 598
TaiI ACGT 1 cut(s) 459
TaqI TCGA 6 cut(s) 96, 142, 459, 705, 714, 942
TasI AATT 6 cut(s) 107, 171, 545, 676, 900, 918
TatI WGTACW 2 cut(s) 74, 541
TfiI GAWTC 2 cut(s) 135, 518
Tru1I TTAA 3 cut(s) 54, 882, 935
Tru9I TTAA 3 cut(s) 54, 882, 935
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 3 cut(s) 28, 325, 427
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 3 cut(s) 164, 303, 774
TspGWI ACGGA 1 cut(s) 940
TspRI CASTG 1 cut(s) 288
Vha464I CTTAAG 1 cut(s) 881
VpaK11BI GGWCC 3 cut(s) 23, 603, 948
XapI RAATTY 2 cut(s) 107, 171
XceI RCATGY 3 cut(s) 249, 500, 890
XmiI GTMKAC 3 cut(s) 459, 511, 714
XspI CTAG 3 cut(s) 116, 366, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.