MD02G1021700.v1.1

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000408
Physical Location & Seq
Reverse (-)
1518408 .. 1529008
10601 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1021700.v1.1.491

Sequence Viewer

Length: 2265 bp
ATGGCACTGGAGTTTATTGGTGGAACACTGTTTACGCAGCTCTACGACGGCGTCAAGCAAGCGATGAGCAAGAGTGGGCGGTTTAAAACGCTTCTCGGAGACCTCAAATCCACGCTAGACTCTTTAGAACCAAGAGACATCCAACAGATTGGAGAGCATAACGTCGAATTGGGTCTCCCAAACGAGGAAATTGAAAGTCTTCAAATGCAAATGGAGGATGGTGTGAAGCTTGTTGGCAAGTTATCCAACTTTCGCATTTGGAACTACTGCTGCTTGGATGATTACGCTCAACAAATTGTTGAGTTGGATAGGGCTCTTAAAAGATTGTTAATAAAATTGAAGATGCAGGAAGCAAGGGATGTGAAGGAGGTCTGGGTTTTGTCGAGGCAAAACCGGGAGAAACTTGATGAAGTTAATAGAAGATTGTTGGATATACGAAAGTTACTGCAGCAGAGAGCGGGGGATGTCGTGGTAGAATTGGTTTCCATAAGTATTACCGAGACAGTGCCCGATCAAGCCCAGGGGAATGTCTCGGAACAAGGGGCTGCTCTACAAGCAACATTTGCAGTGTTGTGCGATGTTGTTATGGAAGTGAAGGACAAGAACACAGCGTTCAAACCTCTCATAGAAAATCTCATGTCCACGCTAGACTCCTTAAGACCGTTAATGGAAGATATACTAGAATATAGAAAACTACTGGATCGCCAAGACGAGTGTAAAAACTTCAGAATACAAATGGAGAAAGGTGTAGAGCTCGTCCACAAGTGCTCCAAAGTTGGTATGTGGACTGGTTGCAAAAGTTATAAATACAGCAACAAAATTATTGGATTGGATGAATCTCTTCAGATATTGTTAGGTATACTGAGAGTGCAGGTAGCAAGAGACGTGAGGGAGACGTTTATTTCGATAAGGAATATAGAGGCAGTGATCAAGAGAATTGAAGAAAGTGGTGTGCTGCAAAATCAAACTCAAGCTGACGGTTGGTGTGCATTGCCGGAACCTTCATTGCCTACAATTGGATCGGATGTGCTAAACATGCAGGGAACGAGTGATGCGAGGGAGACATTGGTTTTGTCTGGCACAACAAAAGCAGGTGTCGAAGAAATTGAAGGGATTGGCCCGGAACAGACTCCAAGTGAAATTACAGTGGATTCACCTTCTTCTGAAGTGAGCTCTTATCCTCTCTCTACTACTGTAACGGATTCAGCTTCTTCTTTTGGATTGAGCTCTTATCCTTTCTCTACTACTGTATCAGATTCAGCTTCTTCTTTTGGATTGAGCTCTTTTCCTGCCAGTCCGCTTCAAGTGCCACCGATTCCTATAATTTCAATGTCTTCAAAGCTCAGTAGATTTGGATCAGTACATCTCAGTTTAAAGCAGATTGCAAAAGCAACTCGCAATTTCTCTCGATCGCAACAAGTAGGTGAAGGAAATTTTGGAACTGTATACAAGGCTCGGTTAGATGATGGCCAAGTTGTTTCTATTAAACGTGCAAAGAAGGAACACTTTGAGAATTTGCAAACGGAATTCAGCAGTGAAGTTGAACTGCTGGCCAAAATTGATCATCGAAATCTCGTAAAGCTACTAGGTTATGTTGATGAAGGAAATGAGCGTCTTATCATTACGGAGTATGTGCCGAATGGTACTCTTCGAGAACATCTGGATCGTCAGCATAGGAAAATCCTAAAGTTCAACCAGCGACTTGAAATTGCTATCGATGTTGCTCATGCCCTTACCTATCTTCACACATATGCTGAAAAACAAATCATCCATCGAAACGTGAAGTCCTCCAACATTCTTCTCACAGAAAGCATGAAAGCCAAAGTGGCAGATTTTGGAATTGCAAGGCTCGGCCCGGTGGAAACAGATGAAACACACATTTCAACCGAAGTGAAAGGAACTGTCGGTTATATTGACCCTGAGTATATGAAAACCTATCAACTCACCCCGAAAAGTGATGTTTACTCTTTCGGGATCTTACTAATAGAAATTTTCACTGGCCGTCGTCCAGTGGAGTCGAAGAGACCTGCTGAAGAGCGGGTGACACTCAGATGGGCCTTCAAGAAGTTGAATGAAGGTAGGGTGGCAGAATTGGTTGATCCTTTAATGGAGGAAACTATAGATGCAGAGCTTCTGATGAAAATCTTCGATTTGGGGATCCAATGTGCAGCCCCCATCCGAGATGCTAGGCCGGACATGAAAGCGGTGGGAGAGCAGTTGTGGACAATAAGGGCAGGCTACCTTAGAAGTGTCAAGAGAGGGTAG

Protein Analysis

755

Amino Acids

84.65

Weight (kDa)

5.8

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 137 3.8e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 181 - 308 8.6e-25 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 469 - 678 2.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 471 - 740 5e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 804
AarI CACCTGC 1 cut(s) 1082
Acc36I ACCTGC 3 cut(s) 862, 1082, 2035
AccBSI CCGCTC 2 cut(s) 458, 2038
AccI GTMKAC 2 cut(s) 859, 1446
AciI CCGC 5 cut(s) 79, 458, 1298, 2038, 2204
AclWI GGATC 8 cut(s) 708, 1027, 1363, 1671, 1982, 2093, 2152, 2165
AcoI YGGCCR 3 cut(s) 1468, 1551, 1999
AcsI RAATTY 4 cut(s) 1432, 1513, 1526, 1989
AcuI CTGAAG 4 cut(s) 709, 827, 1185, 2052
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 1362, 1645
AfiI CCNNNNNNNGG 2 cut(s) 184, 1016
AflII CTTAAG 1 cut(s) 655
AjiI CACGTC 1 cut(s) 886
AjnI CCWGG 1 cut(s) 519
AjuI GAANNNNNNNTTGG 2 cut(s) 1419, 1451
Alw21I GWGCWC 5 cut(s) 756, 770, 1175, 1229, 1283
Alw26I GTCTC 9 cut(s) 93, 129, 179, 494, 535, 876, 887, 1055, 2017
AlwI GGATC 8 cut(s) 708, 1027, 1363, 1671, 1982, 2093, 2152, 2165
AoxI GGCC 7 cut(s) 1117, 1468, 1551, 1852, 1999, 2055, 2189
ApeKI GCWGC 6 cut(s) 37, 270, 448, 545, 955, 2168
ApoI RAATTY 4 cut(s) 1432, 1513, 1526, 1989
ArsI GACNNNNNNTTYG 4 cut(s) 886, 918, 1054, 1086
Asp700I GAANNNNTTC 3 cut(s) 198, 840, 2144
AspS9I GGNCC 3 cut(s) 1118, 1853, 2055
AsuC2I CCSGG 3 cut(s) 395, 1121, 1856
AsuHPI GGTGA 4 cut(s) 1146, 1436, 1936, 2053
BaeGI GKGCMC 1 cut(s) 510
BaeI ACNNNNGTAYC 2 cut(s) 1233, 1266
BalI TGGCCA 2 cut(s) 1470, 1553
BamHI GGATCC 1 cut(s) 2157
BanII GRGCYC 5 cut(s) 316, 756, 1175, 1229, 1283
BbsI GAAGAC 2 cut(s) 191, 1326
Bbv12I GWGCWC 5 cut(s) 756, 770, 1175, 1229, 1283
BbvI GCAGC 6 cut(s) 49, 257, 460, 532, 942, 2180
BccI CCATC 5 cut(s) 212, 1460, 1779, 2046, 2183
BceAI ACGGC 2 cut(s) 64, 1986
BciT130I CCWGG 1 cut(s) 521
BclI TGATCA 2 cut(s) 927, 1561
BcnI CCSGG 3 cut(s) 395, 1121, 1856
BcoDI GTCTC 9 cut(s) 93, 129, 179, 494, 535, 876, 887, 1055, 2017
BfaI CTAG 5 cut(s) 116, 647, 680, 1586, 2187
BfmI CTRYAG 2 cut(s) 446, 2118
BfrI CTTAAG 1 cut(s) 655
BfuAI ACCTGC 3 cut(s) 862, 1082, 2035
BglI GCCNNNNNGGC 1 cut(s) 1826
BisI GCNGC 6 cut(s) 38, 271, 449, 546, 956, 2169
BlsI GCNGC 6 cut(s) 39, 272, 450, 547, 957, 2170
Bme1390I CCNGG 4 cut(s) 395, 521, 1121, 1856
BmgBI CACGTC 1 cut(s) 886
BmgT120I GGNCC 3 cut(s) 1118, 1853, 2055
BmiI GGNNCC 2 cut(s) 999, 2159
BmrFI CCNGG 4 cut(s) 395, 521, 1121, 1856
BmsI GCATC 4 cut(s) 333, 1042, 2113, 2173
BpiI GAAGAC 2 cut(s) 191, 1326
BpmI CTGGAG 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 954
BpuMI CCSGG 3 cut(s) 395, 1121, 1856
Bsa29I ATCGAT 1 cut(s) 1716
BsaBI GATNNNNATC 2 cut(s) 1354, 2141
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 3 cut(s) 93, 179, 2017
BsaJI CCNNGG 2 cut(s) 519, 520
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 1016
Bse1I ACTGG 6 cut(s) 12, 702, 793, 1293, 2002, 2009
Bse3DI GCAATG 2 cut(s) 989, 1004
Bse8I GATNNNNATC 2 cut(s) 1354, 2141
BseBI CCWGG 1 cut(s) 521
BseCI ATCGAT 1 cut(s) 1716
BseDI CCNNGG 2 cut(s) 519, 520
BseGI GGATG 9 cut(s) 138, 223, 283, 364, 469, 838, 1030, 1767, 2175
BseJI GATNNNNATC 2 cut(s) 1354, 2141
BseLI CCNNNNNNNGG 2 cut(s) 184, 1016
BseMI GCAATG 2 cut(s) 989, 1004
BseMII CTCAG 5 cut(s) 854, 1357, 1381, 1911, 2062
BseNI ACTGG 6 cut(s) 12, 702, 793, 1293, 2002, 2009
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 6 cut(s) 49, 257, 460, 532, 942, 2180
BsgI GTGCAG 2 cut(s) 890, 2187
Bsh1285I CGRYCG 1 cut(s) 1412
BshFI GGCC 7 cut(s) 1119, 1470, 1553, 1854, 2001, 2057, 2191
BshVI ATCGAT 1 cut(s) 1716
BsiEI CGRYCG 1 cut(s) 1412
BsiHKAI GWGCWC 5 cut(s) 756, 770, 1175, 1229, 1283
BsiSI CCGG 5 cut(s) 394, 995, 1121, 1856, 2192
BslI CCNNNNNNNGG 2 cut(s) 184, 1016
BsmAI GTCTC 9 cut(s) 93, 129, 179, 494, 535, 876, 887, 1055, 2017
BsmBI CGTCTC 2 cut(s) 876, 887
BsnI GGCC 7 cut(s) 1119, 1470, 1553, 1854, 2001, 2057, 2191
Bso31I GGTCTC 3 cut(s) 93, 179, 2017
Bsp1286I GDGCHC 7 cut(s) 316, 510, 756, 770, 1175, 1229, 1283
BspACI CCGC 5 cut(s) 79, 458, 1298, 2038, 2204
BspANI GGCC 7 cut(s) 1119, 1470, 1553, 1854, 2001, 2057, 2191
BspCNI CTCAG 5 cut(s) 855, 1356, 1380, 1912, 2061
BspDI ATCGAT 1 cut(s) 1716
BspLI GGNNCC 2 cut(s) 999, 2159
BspMAI CTGCAG 1 cut(s) 450
BspMI ACCTGC 3 cut(s) 862, 1082, 2035
BspPI GGATC 8 cut(s) 708, 1027, 1363, 1671, 1982, 2093, 2152, 2165
BspQI GCTCTTC 1 cut(s) 2028
BspTI CTTAAG 1 cut(s) 655
BspTNI GGTCTC 3 cut(s) 93, 179, 2017
BsrBI CCGCTC 2 cut(s) 458, 2038
BsrDI GCAATG 2 cut(s) 989, 1004
BsrI ACTGG 6 cut(s) 12, 702, 793, 1293, 2002, 2009
BssECI CCNNGG 2 cut(s) 519, 520
BssNAI GTATAC 2 cut(s) 860, 1447
BssNI GRCGYC 1 cut(s) 51
Bst1107I GTATAC 2 cut(s) 860, 1447
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 9 cut(s) 30, 505, 663, 980, 1147, 1195, 1249, 1444, 1903
Bst6I CTCTTC 4 cut(s) 846, 1653, 2015, 2028
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 655
BstAPI GCANNNNNTGC 1 cut(s) 563
BstC8I GCNNGC 3 cut(s) 60, 1551, 2236
BstDEI CTNAG 6 cut(s) 863, 1343, 1367, 1920, 2048, 2243
BstF5I GGATG 9 cut(s) 138, 223, 283, 364, 469, 838, 1030, 1767, 2175
BstMAI GTCTC 9 cut(s) 93, 129, 179, 494, 535, 876, 887, 1055, 2017
BstMCI CGRYCG 1 cut(s) 1412
BstMWI GCNNNNNNNGC 5 cut(s) 554, 563, 1036, 1306, 1826
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 1 cut(s) 1039
BstSCI CCNGG 4 cut(s) 393, 519, 1119, 1854
BstSFI CTRYAG 2 cut(s) 446, 2118
BstSLI GKGCMC 1 cut(s) 510
BstV1I GCAGC 6 cut(s) 49, 257, 460, 532, 942, 2180
BstV2I GAAGAC 2 cut(s) 191, 1326
BstX2I RGATCY 2 cut(s) 1974, 2157
BstXI CCANNNNNNTGG 1 cut(s) 149
BstYI RGATCY 2 cut(s) 1974, 2157
BstZ17I GTATAC 2 cut(s) 860, 1447
Bsu15I ATCGAT 1 cut(s) 1716
BsuRI GGCC 7 cut(s) 1119, 1470, 1553, 1854, 2001, 2057, 2191
BsuTUI ATCGAT 1 cut(s) 1716
BtgZI GCGATG 2 cut(s) 77, 591
BtrI CACGTC 1 cut(s) 886
BtsCI GGATG 9 cut(s) 138, 223, 283, 364, 469, 838, 1030, 1767, 2175
BtsI GCAGTG 3 cut(s) 573, 930, 1540
BtsIMutI CAGTG 9 cut(s) 5, 26, 510, 573, 930, 1152, 1540, 1995, 2016
BveI ACCTGC 3 cut(s) 862, 1082, 2035
Cac8I GCNNGC 3 cut(s) 60, 1551, 2236
Cfr13I GGNCC 3 cut(s) 1118, 1853, 2055
ClaI ATCGAT 1 cut(s) 1716
CseI GACGC 2 cut(s) 40, 1601
Csp6I GTAC 2 cut(s) 1361, 1644
CviAII CATG 5 cut(s) 637, 1036, 1727, 1813, 2197
CviQI GTAC 2 cut(s) 1361, 1644
DdeI CTNAG 6 cut(s) 863, 1343, 1367, 1920, 2048, 2243
DraI TTTAAA 2 cut(s) 85, 1374
EaeI YGGCCR 3 cut(s) 1468, 1551, 1999
Eam1104I CTCTTC 4 cut(s) 846, 1653, 2015, 2028
EarI CTCTTC 4 cut(s) 846, 1653, 2015, 2028
Ecl136II GAGCTC 4 cut(s) 754, 1173, 1227, 1281
Eco24I GRGCYC 5 cut(s) 316, 756, 1175, 1229, 1283
Eco31I GGTCTC 3 cut(s) 93, 179, 2017
Eco53kI GAGCTC 4 cut(s) 754, 1173, 1227, 1281
Eco57I CTGAAG 4 cut(s) 709, 827, 1185, 2052
EcoICRI GAGCTC 4 cut(s) 754, 1173, 1227, 1281
EcoRI GAATTC 1 cut(s) 1526
EcoRII CCWGG 1 cut(s) 519
EcoT38I GRGCYC 5 cut(s) 316, 756, 1175, 1229, 1283
Esp3I CGTCTC 2 cut(s) 876, 887
FaeI CATG 5 cut(s) 640, 1039, 1730, 1816, 2200
FalI AAGNNNNNCTT 2 cut(s) 1490, 1522
FatI CATG 5 cut(s) 636, 1035, 1726, 1812, 2196
FauI CCCGC 2 cut(s) 451, 2031
FauNDI CATATG 1 cut(s) 1750
FbaI TGATCA 2 cut(s) 927, 1561
FblI GTMKAC 2 cut(s) 859, 1446
Fnu4HI GCNGC 6 cut(s) 38, 271, 449, 546, 956, 2169
FokI GGATG 9 cut(s) 125, 230, 290, 371, 476, 845, 1037, 1754, 2162
FriOI GRGCYC 5 cut(s) 316, 756, 1175, 1229, 1283
Fsp4HI GCNGC 6 cut(s) 38, 271, 449, 546, 956, 2169
FspBI CTAG 5 cut(s) 116, 647, 680, 1586, 2187
GluI GCNGC 6 cut(s) 38, 271, 449, 546, 956, 2169
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 7 cut(s) 1119, 1470, 1553, 1854, 2001, 2057, 2191
HapII CCGG 5 cut(s) 394, 995, 1121, 1856, 2192
HgaI GACGC 2 cut(s) 40, 1601
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 5 cut(s) 640, 1039, 1730, 1816, 2200
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 9 cut(s) 119, 650, 836, 1129, 1151, 1202, 1256, 1315, 2015
HpaII CCGG 5 cut(s) 394, 995, 1121, 1856, 2192
HphI GGTGA 4 cut(s) 1146, 1436, 1936, 2053
Hpy166II GTNNAC 8 cut(s) 33, 642, 760, 786, 860, 1447, 1963, 2223
Hpy188III TCNNGA 7 cut(s) 931, 1407, 1652, 1661, 1972, 2062, 2254
Hpy8I GTNNAC 8 cut(s) 33, 642, 760, 786, 860, 1447, 1963, 2223
Hpy99I CGWCG 3 cut(s) 50, 167, 2007
HpyCH4III ACNGT 9 cut(s) 30, 505, 663, 980, 1147, 1195, 1249, 1444, 1903
HpyCH4IV ACGT 5 cut(s) 162, 885, 896, 1489, 1779
HpyF10VI GCNNNNNNNGC 5 cut(s) 554, 563, 1036, 1306, 1826
HpyF3I CTNAG 6 cut(s) 863, 1343, 1367, 1920, 2048, 2243
HpySE526I ACGT 5 cut(s) 162, 885, 896, 1489, 1779
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 5 cut(s) 640, 1039, 1730, 1816, 2200
Ksp22I TGATCA 2 cut(s) 927, 1561
LguI GCTCTTC 1 cut(s) 2028
LmnI GCTCC 1 cut(s) 773
Lsp1109I GCAGC 6 cut(s) 49, 257, 460, 532, 942, 2180
LweI GCATC 4 cut(s) 333, 1042, 2113, 2173
MaeI CTAG 5 cut(s) 116, 647, 680, 1586, 2187
MaeII ACGT 5 cut(s) 162, 885, 896, 1489, 1779
MaeIII GTNAC 3 cut(s) 441, 1195, 2041
MbiI CCGCTC 2 cut(s) 458, 2038
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 2 cut(s) 1974, 2157
MhlI GDGCHC 7 cut(s) 316, 510, 756, 770, 1175, 1229, 1283
MlsI TGGCCA 2 cut(s) 1470, 1553
MluNI TGGCCA 2 cut(s) 1470, 1553
MlyI GAGTC 4 cut(s) 113, 644, 1123, 2024
MmeI TCCRAC 5 cut(s) 166, 270, 285, 408, 1815
Mox20I TGGCCA 2 cut(s) 1470, 1553
MroXI GAANNNNTTC 3 cut(s) 198, 840, 2144
MscI TGGCCA 2 cut(s) 1470, 1553
MseI TTAA 9 cut(s) 84, 318, 329, 414, 656, 665, 1373, 1485, 2105
MslI CAYNNNNRTG 2 cut(s) 1749, 2050
Msp20I TGGCCA 2 cut(s) 1470, 1553
MspCI CTTAAG 1 cut(s) 655
MspI CCGG 5 cut(s) 394, 995, 1121, 1856, 2192
MspR9I CCNGG 4 cut(s) 395, 521, 1121, 1856
MunI CAATTG 1 cut(s) 1014
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 5 cut(s) 554, 563, 1036, 1306, 1826
NciI CCSGG 3 cut(s) 395, 1121, 1856
NdeI CATATG 1 cut(s) 1750
NlaIII CATG 5 cut(s) 640, 1039, 1730, 1816, 2200
NlaIV GGNNCC 2 cut(s) 999, 2159
NmeAIII GCCGAG 1 cut(s) 1830
NmuCI GTSAC 1 cut(s) 2041
NspI RCATGY 1 cut(s) 1039
PaqCI CACCTGC 1 cut(s) 1082
PasI CCCWGGG 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 2028
PcsI WCGNNNNNNNCGW 2 cut(s) 902, 1052
PdmI GAANNNNTTC 3 cut(s) 198, 840, 2144
PfeI GAWTC 5 cut(s) 836, 1151, 1202, 1256, 1315
PflFI GACNNNGTC 1 cut(s) 50
PkrI GCNGC 6 cut(s) 39, 272, 450, 547, 957, 2170
Ple19I CGATCG 1 cut(s) 1412
PleI GAGTC 4 cut(s) 113, 644, 1123, 2023
PpsI GAGTC 4 cut(s) 113, 644, 1123, 2023
PsiI TTATAA 1 cut(s) 804
Psp124BI GAGCTC 4 cut(s) 756, 1175, 1229, 1283
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 2 cut(s) 999, 2159
PspPI GGNCC 3 cut(s) 1118, 1853, 2055
PsrI GAACNNNNNNTAC 2 cut(s) 16, 48
PstI CTGCAG 1 cut(s) 450
PsuI RGATCY 2 cut(s) 1974, 2157
PsyI GACNNNGTC 1 cut(s) 50
PvuI CGATCG 1 cut(s) 1412
RsaI GTAC 2 cut(s) 1362, 1645
RsaNI GTAC 2 cut(s) 1361, 1644
RseI CAYNNNNRTG 2 cut(s) 1749, 2050
SacI GAGCTC 4 cut(s) 756, 1175, 1229, 1283
SapI GCTCTTC 1 cut(s) 2028
SaqAI TTAA 9 cut(s) 84, 318, 329, 414, 656, 665, 1373, 1485, 2105
SatI GCNGC 6 cut(s) 38, 271, 449, 546, 956, 2169
Sau96I GGNCC 3 cut(s) 1118, 1853, 2055
SchI GAGTC 4 cut(s) 113, 644, 1123, 2024
ScrFI CCNGG 4 cut(s) 395, 521, 1121, 1856
SduI GDGCHC 7 cut(s) 316, 510, 756, 770, 1175, 1229, 1283
SfaNI GCATC 4 cut(s) 333, 1042, 2113, 2173
SfcI CTRYAG 2 cut(s) 446, 2118
SmiMI CAYNNNNRTG 2 cut(s) 1749, 2050
SmlI CTYRAG 2 cut(s) 655, 969
SmoI CTYRAG 2 cut(s) 655, 969
SsiI CCGC 5 cut(s) 79, 458, 1298, 2038, 2204
SspMI CTAG 5 cut(s) 116, 647, 680, 1586, 2187
SstI GAGCTC 4 cut(s) 756, 1175, 1229, 1283
StyD4I CCNGG 4 cut(s) 393, 519, 1119, 1854
TaaI ACNGT 9 cut(s) 30, 505, 663, 980, 1147, 1195, 1249, 1444, 1903
TaiI ACGT 5 cut(s) 165, 888, 899, 1492, 1782
TatI WGTACW 1 cut(s) 1360
TfiI GAWTC 5 cut(s) 836, 1151, 1202, 1256, 1315
Tru1I TTAA 9 cut(s) 84, 318, 329, 414, 656, 665, 1373, 1485, 2105
Tru9I TTAA 9 cut(s) 84, 318, 329, 414, 656, 665, 1373, 1485, 2105
TscAI CASTG 9 cut(s) 12, 33, 510, 573, 930, 1152, 1540, 2002, 2016
TseFI GTSAC 1 cut(s) 2041
TseI GCWGC 6 cut(s) 37, 270, 448, 545, 955, 2168
Tsp45I GTSAC 1 cut(s) 2041
TspGWI ACGGA 3 cut(s) 1214, 1538, 1640
TspRI CASTG 9 cut(s) 12, 33, 510, 573, 930, 1152, 1540, 2002, 2016
Tth111I GACNNNGTC 1 cut(s) 50
Vha464I CTTAAG 1 cut(s) 655
XapI RAATTY 4 cut(s) 1432, 1513, 1526, 1989
XceI RCATGY 1 cut(s) 1039
XmiI GTMKAC 2 cut(s) 859, 1446
XmnI GAANNNNTTC 3 cut(s) 198, 840, 2144
XspI CTAG 5 cut(s) 116, 647, 680, 1586, 2187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.