Rh2BG180800

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000408
Physical Location & Seq
Forward (+)
16087162 .. 16091852
4691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG180800.1

Sequence Viewer

Length: 2820 bp
ATGGCAGTGGAGTTACTTGGAGGAGCAGTGTTTACACTGCTGTATGATGGGGTTAAGCAAGCAATCGGAAAGACAGGGACTTTCAAATACCTTCTCAAAGACATCAAATCCACACTGGAGTCTTTGAACAAGCTACACGCCACGGCCATCAGACAAATCGGAGAGTATAACCTGGAATTGGGTCTCCCAAATGATGATATAGAAGAGCTCAAAGAGCAAATGGAGGAAGGGGCAAAGCTGGTGGGAAAGTTGTCTGATTTTCGTGTGTGGAACTACTGCTGCTTAAGTTGTTATACCGATCAACTTGTTGATTTGGATAGGTCTCTCAAGAGGCTGTTGGAGAATCTTAAGTTGCAAGAAACTAGAGATGTGAAAGAGATCTTGGATTTGCTGAGGAAATACCGCGTTGAGCTTGATGAGTTGAAGGGAACGTTGCAGATATTACTGCAGCAGAGAGCAGGAGATGTGAAGGAGACCACCTCGAATTCAACTGCAGGAAGCAGTACAGAGACCATGCCTCAGCAAAATGTAGGGAACGGAGAGGAACAAGGAGGGGCAAATCTTCGTGCAGTGTATACGATGCTGTTTAACGTTGTTACAGAAGTGAAGGACAAGACTTTGATGTTCAGACCCCTACTCGGAGATCTCAAGTCCATGCTAGATTCCTTAGAACCACTGATCAAAGAGATAGATCAATACAATAGCGTATTGGTTCTCCCAAAGCCGGAGGTAGAAAAGTTCAGGACACAAATGGACAAGGGTGTGGAGCTCGTTTGCAAGTGCTCCAAGGTTCGTCCGTGGACCAGCTACAAAAAGTACAATTACACAATCCGACTTGTTGGATTGAATGAATCTGTTCGATTACTGTTAAGTAGACTGAGAGAGCAGGTAGCTGGGGAGGTGAGTGAGACATTGGCTTCAATAAGTAACCTAAAGGAGGTAATCAAGCAATATGAAGGGCCTAGTGTCATACAAAATCAACATGAGATTATAGGTTCCTGCAAAGTACCTGAACCTTCATCGCCTACATTGATGCCTGAGATTGAGGGTTCTGATGAAGTGCATCCATTACCAGTGCCTCCTGGAGCAAAACTACCTTCACCAAAGGAGGAAGTTGGGGAAGTGAGCGAGACATTGGCTTCAATAAGTAACCTAAAGGAGGTAGTCAAGCAATATGAAGGGCCTAGTGTCATACAAAATCAACATGAGATTATAGGTTCCTGCAAAGTACCCGAACCTTCATCGCCTACAGTGATGCCTGAGATTGAGGATTCTGTTGAAGTGCATCCATTACCAGTGCCTCCTGGAGCAAAACTACCTTCACCAAAGGTTCTAATTACATCTGAACCGGAGTCCCAGCCAATGAAAGGTCAATGGCAAAAGGGAAATCTTATTGGTCGTGGCACGTTTGGGAATGTTTATGTTGCTTCCAATGGGTATATACAGTTTCTTGTACAGGCGATTGAAATCAAAGTCCTCAGCCGGCTCAAGCATCCTAATATTGTGCAATATTATGGGACCGAGATAGTTGGAGATAGATTTTTTATATATCAGGAGTATGTCCATCCTGTATCTATTAACAGATACATCCAAGAATATTGTGGAGCCATGACAGAAGCTGTTGTTCGCAGTTTAACTCACCAGATAGTGTCTGGGTTGGCTTACTTGCACAGCAAAGAAACGATACACGGGGACATCAAAGGGGATAATTTGCTCGTTGAGTCATCTGGTGTTGTCAAGATAGCTGACTTTGGAATTGTAAAACATCTTCAAGGAAGACTCCTAAAGGGAGGTCCATACTGGATGGCTCCAGAGATCATCAAAAATGGGATACCGAACGATAAATGCTCTGATCATGATTTGGCTCGTGGTGGTGACATATGGAGTTTGGGTTGCACTGTTATTGAATTGTTCACTGGAAAACCTCCCTGGAGTGAGTTCGAACAGCTTCAAGCCATGTTTCTAATTGTTCGTAAAGATCCTCCAATTCCAGAAACATTGTCACCTGAGGGTAAGGATTTCCTAACATGCTGCTTGCAGAGAAACCCTGCAGAGAGGCCAACTGCTGCAATGCTTTTAGAACATCCATTCTTAAAGAACTCCCAGCCGCAGGATGTCCCAACTATGACCGGTGCCATTAATCTGGTGAATTTGATGGATAAACACAAAATGACGGAACTCCAATCGTACTTGAAAGAAGACTTGATCGTTGAGATTGAGAAGATCAGGTTCTCCACTGATCCATCAATCCATCACCGCGGGATGTCTCTCGAAAGAGGCAAGGAATTCTCAGGAGGACAAGGATCGTCATTGGGGTACTCGAGTCCGAGTCCCACCGATCATCATGACCAGCAGCAGCAGCCAGTGACGCTCAGCAGGCATGAGATGCAGAAGAGGAGGGACTGGAACACTTTCGGGCAGTACTTGAAGAACCAGAGGCCCCCAGTGCCTCCCTCCCAGTGCAATTACAGCCATGTTCTCGAGTTCCTCAGGTACCTCGACCAGTTCGGGAAGACCAAAGTCCATCTCCAGGGTTGTGTGTTTTACGGCCAGCCGGAGCCACCTGCGCCTTGCACCTGTCCTCTCCGGCAGGCCTGGGGAAGCCTGGATGCTCTCATAGGGAGGCTGAGGGCTGCTTATGAGGAGAACGGTGGGGCCCCTGAGAGCAATCCTTTTGCTAGTGGTGCAATCAGGGGGTACTTGAGGGAGGTCAGGGAGTGTCAGGCCAAGGCTAGAGGGATTCCTTACAATAATAAGAAGAAGAAGCCTGGAAATAGTGATCGTGATTCTTCAACAAGTTCTATCATGCACTTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

939

Amino Acids

105.0

Weight (kDa)

6.18

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 140 1.1e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 184 - 291 4.4e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 489 - 698 3.7e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 489 - 694 1.1e-34 Protein tyrosine and serine/threonine kinase
ALOG_dom PF04852 783 - 906 2.8e-62 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2572
Acc36I ACCTGC 2 cut(s) 877, 2572
Acc65I GGTACC 1 cut(s) 2493
AccB1I GGYRCC 2 cut(s) 2132, 2493
AccI GTMKAC 2 cut(s) 575, 874
AccII CGCG 2 cut(s) 405, 2259
AciI CCGC 4 cut(s) 403, 2108, 2257, 2259
AclI AACGTT 2 cut(s) 431, 591
AclWI GGATC 3 cut(s) 1973, 2234, 2311
AcoI YGGCCR 2 cut(s) 144, 2548
AcsI RAATTY 3 cut(s) 484, 2149, 2285
AfiI CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2110, 2530
AflII CTTAAG 2 cut(s) 283, 347
AgeI ACCGGT 1 cut(s) 2129
AjnI CCWGG 8 cut(s) 171, 1081, 1303, 1928, 2529, 2594, 2604, 2767
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
Alw21I GWGCWC 3 cut(s) 210, 771, 785
Alw26I GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2271
AlwI GGATC 3 cut(s) 1973, 2234, 2311
AlwNI CAGNNNCTG 1 cut(s) 1619
Ama87I CYCGRG 2 cut(s) 2320, 2480
AoxI GGCC 9 cut(s) 144, 959, 1181, 2057, 2438, 2548, 2592, 2655, 2724
ApaI GGGCCC 1 cut(s) 2659
ApeKI GCWGC 8 cut(s) 279, 448, 2031, 2066, 2353, 2356, 2359, 2633
ApoI RAATTY 3 cut(s) 484, 2149, 2285
AseI ATTAAT 1 cut(s) 2139
AsiGI ACCGGT 1 cut(s) 2129
Asp700I GAANNNNTTC 3 cut(s) 855, 1947, 2411
Asp718I GGTACC 1 cut(s) 2493
AspLEI GCGC 1 cut(s) 2569
AspS9I GGNCC 8 cut(s) 801, 959, 1181, 1518, 1793, 2439, 2655, 2656
AsuHPI GGTGA 8 cut(s) 913, 1092, 1314, 1631, 1886, 1995, 2158, 2246
AsuII TTCGAA 1 cut(s) 1941
AvaI CYCGRG 2 cut(s) 2320, 2480
AvaII GGWCC 3 cut(s) 801, 1518, 1793
AxyI CCTNAGG 2 cut(s) 2007, 2489
BaeGI GKGCMC 1 cut(s) 2659
BaeI ACNNNNGTAYC 2 cut(s) 2308, 2341
BanI GGYRCC 2 cut(s) 2132, 2493
BanII GRGCYC 3 cut(s) 210, 771, 2659
BarI GAAGNNNNNNTAC 2 cut(s) 1752, 1784
BauI CACGAG 1 cut(s) 1866
BbsI GAAGAC 3 cut(s) 1783, 2205, 2519
Bbv12I GWGCWC 3 cut(s) 210, 771, 785
BbvCI CCTCAGC 4 cut(s) 392, 519, 1478, 2627
BbvI GCAGC 8 cut(s) 266, 460, 2018, 2053, 2365, 2368, 2371, 2620
BccI CCATC 8 cut(s) 41, 155, 1572, 1798, 2149, 2251, 2259, 2532
BceAI ACGGC 2 cut(s) 159, 2563
BciT130I CCWGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
BciVI GTATCC 1 cut(s) 1824
BclI TGATCA 2 cut(s) 678, 1852
BcoDI GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2271
BfaI CTAG 6 cut(s) 363, 659, 963, 1185, 2679, 2733
BfmI CTRYAG 4 cut(s) 446, 492, 1248, 2049
BfrI CTTAAG 2 cut(s) 283, 347
BfuAI ACCTGC 2 cut(s) 877, 2572
BfuI GTATCC 1 cut(s) 1824
BglII AGATCT 2 cut(s) 378, 643
BisI GCNGC 9 cut(s) 280, 449, 2032, 2067, 2108, 2354, 2357, 2360, 2634
BlpI GCTNAGC 1 cut(s) 2372
BlsI GCNGC 9 cut(s) 281, 450, 2033, 2068, 2109, 2355, 2358, 2361, 2635
BmcAI AGTACT 1 cut(s) 2423
Bme1390I CCNGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
Bme18I GGWCC 3 cut(s) 801, 1518, 1793
BmeT110I CYCGRG 2 cut(s) 2320, 2480
BmgT120I GGNCC 8 cut(s) 801, 959, 1181, 1518, 1793, 2439, 2655, 2656
BmrFI CCNGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
BmrI ACTGGG 2 cut(s) 2438, 2452
BmsI GCATC 8 cut(s) 570, 1023, 1072, 1245, 1294, 1501, 2376, 2599
BmuI ACTGGG 2 cut(s) 2438, 2452
BoxI GACNNNNGTC 1 cut(s) 2519
BpiI GAAGAC 3 cut(s) 1783, 2205, 2519
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 6 cut(s) 137, 1104, 1326, 1794, 1951, 2513
Bpu10I CCTNAGC 4 cut(s) 392, 519, 1478, 2627
Bpu1102I GCTNAGC 1 cut(s) 2372
Bpu14I TTCGAA 1 cut(s) 1941
BpuEI CTTGAG 4 cut(s) 311, 632, 1472, 2722
BsaBI GATNNNNATC 1 cut(s) 1466
BsaI GGTCTC 4 cut(s) 188, 327, 467, 503
BsaJI CCNNGG 8 cut(s) 141, 786, 797, 1928, 2257, 2530, 2595, 2727
BsaWI WCCGGW 2 cut(s) 1348, 2129
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 1876, 1906
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2110, 2530
Bse118I RCCGGY 2 cut(s) 1482, 2129
Bse21I CCTNAGG 2 cut(s) 2007, 2489
Bse3DI GCAATG 1 cut(s) 2076
Bse8I GATNNNNATC 1 cut(s) 1466
BseBI CCWGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
BseDI CCNNGG 8 cut(s) 141, 786, 797, 1928, 2257, 2530, 2595, 2727
BseJI GATNNNNATC 1 cut(s) 1466
BseLI CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2110, 2530
BseMI GCAATG 1 cut(s) 2076
BseRI GAGGAG 3 cut(s) 36, 2410, 2657
BseSI GKGCMC 1 cut(s) 2659
BseXI GCAGC 8 cut(s) 266, 460, 2018, 2053, 2365, 2368, 2371, 2620
BseYI CCCAGC 3 cut(s) 893, 1356, 2103
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 2 cut(s) 405, 2259
BshFI GGCC 9 cut(s) 146, 961, 1183, 2059, 2440, 2550, 2594, 2657, 2726
BshNI GGYRCC 2 cut(s) 2132, 2493
BshTI ACCGGT 1 cut(s) 2129
BsiHKAI GWGCWC 3 cut(s) 210, 771, 785
BsiHKCI CYCGRG 2 cut(s) 2320, 2480
BsiSI CCGG 6 cut(s) 725, 1349, 1483, 2130, 2555, 2587
BslFI GGGAC 7 cut(s) 91, 1339, 1531, 1706, 2102, 2316, 2414
BslI CCNNNNNNNGG 8 cut(s) 178, 638, 724, 937, 1159, 1367, 2110, 2530
BsmAI GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2271
BsmFI GGGAC 7 cut(s) 91, 1339, 1531, 1706, 2102, 2316, 2414
BsnI GGCC 9 cut(s) 146, 961, 1183, 2059, 2440, 2550, 2594, 2657, 2726
Bso31I GGTCTC 4 cut(s) 188, 327, 467, 503
BsoBI CYCGRG 2 cut(s) 2320, 2480
Bsp119I TTCGAA 1 cut(s) 1941
Bsp120I GGGCCC 1 cut(s) 2655
Bsp1286I GDGCHC 4 cut(s) 210, 771, 785, 2659
Bsp1407I TGTACA 1 cut(s) 1453
Bsp1720I GCTNAGC 1 cut(s) 2372
BspACI CCGC 4 cut(s) 403, 2108, 2257, 2259
BspANI GGCC 9 cut(s) 146, 961, 1183, 2059, 2440, 2550, 2594, 2657, 2726
BspFNI CGCG 2 cut(s) 405, 2259
BspHI TCATGA 2 cut(s) 1855, 2344
BspMAI CTGCAG 3 cut(s) 450, 496, 2053
BspMI ACCTGC 2 cut(s) 877, 2572
BspPI GGATC 3 cut(s) 1973, 2234, 2311
BspQI GCTCTTC 1 cut(s) 198
BspT104I TTCGAA 1 cut(s) 1941
BspT107I GGYRCC 2 cut(s) 2132, 2493
BspTI CTTAAG 2 cut(s) 283, 347
BspTNI GGTCTC 4 cut(s) 188, 327, 467, 503
BsrDI GCAATG 1 cut(s) 2076
BsrFI RCCGGY 2 cut(s) 1482, 2129
BsrGI TGTACA 1 cut(s) 1453
BssAI RCCGGY 2 cut(s) 1482, 2129
BssECI CCNNGG 8 cut(s) 141, 786, 797, 1928, 2257, 2530, 2595, 2727
BssNAI GTATAC 1 cut(s) 576
BssSI CACGAG 1 cut(s) 1866
BssT1I CCWWGG 2 cut(s) 786, 2727
Bst1107I GTATAC 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 1866
Bst2UI CCWGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
Bst4CI ACNGT 5 cut(s) 867, 1252, 1446, 1900, 2651
Bst6I CTCTTC 2 cut(s) 198, 2387
BstAFI CTTAAG 2 cut(s) 283, 347
BstAPI GCANNNNNTGC 1 cut(s) 2386
BstAUI TGTACA 1 cut(s) 1453
BstBI TTCGAA 1 cut(s) 1941
BstC8I GCNNGC 6 cut(s) 60, 1484, 2036, 2378, 2552, 2592
BstDSI CCRYGG 3 cut(s) 141, 797, 2257
BstENI CCTNNNNNAGG 2 cut(s) 935, 1157
BstFNI CGCG 2 cut(s) 405, 2259
BstHHI GCGC 1 cut(s) 2569
BstMAI GTCTC 7 cut(s) 188, 327, 467, 503, 902, 1124, 2271
BstMWI GCNNNNNNNGC 9 cut(s) 214, 2359, 2368, 2377, 2386, 2446, 2469, 2566, 2684
BstNI CCWGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
BstNSI RCATGY 1 cut(s) 2031
BstPAI GACNNNNGTC 1 cut(s) 2519
BstSCI CCNGG 8 cut(s) 171, 1081, 1303, 1928, 2529, 2594, 2604, 2767
BstSFI CTRYAG 4 cut(s) 446, 492, 1248, 2049
BstSLI GKGCMC 1 cut(s) 2659
BstUI CGCG 2 cut(s) 405, 2259
BstV1I GCAGC 8 cut(s) 266, 460, 2018, 2053, 2365, 2368, 2371, 2620
BstV2I GAAGAC 3 cut(s) 1783, 2205, 2519
BstX2I RGATCY 3 cut(s) 378, 643, 1978
BstXI CCANNNNNNTGG 1 cut(s) 2143
BstYI RGATCY 3 cut(s) 378, 643, 1978
BstZ17I GTATAC 1 cut(s) 576
Bsu36I CCTNAGG 2 cut(s) 2007, 2489
BsuI GTATCC 1 cut(s) 1824
BsuRI GGCC 9 cut(s) 146, 961, 1183, 2059, 2440, 2550, 2594, 2657, 2726
BtgI CCRYGG 3 cut(s) 141, 797, 2257
BtgZI GCGATG 2 cut(s) 1005, 1227
BtsI GCAGTG 4 cut(s) 12, 33, 35, 576
BveI ACCTGC 2 cut(s) 877, 2572
Cac8I GCNNGC 6 cut(s) 60, 1484, 2036, 2378, 2552, 2592
CaiI CAGNNNCTG 1 cut(s) 1619
CciI TCATGA 2 cut(s) 1855, 2344
CfoI GCGC 1 cut(s) 2569
Cfr10I RCCGGY 2 cut(s) 1482, 2129
Cfr13I GGNCC 8 cut(s) 801, 959, 1181, 1518, 1793, 2439, 2655, 2656
Cfr42I CCGCGG 1 cut(s) 2260
CseI GACGC 1 cut(s) 2377
CspAI ACCGGT 1 cut(s) 2129
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
EaeI YGGCCR 2 cut(s) 144, 2548
Eam1104I CTCTTC 2 cut(s) 198, 2387
EarI CTCTTC 2 cut(s) 198, 2387
Ecl136II GAGCTC 2 cut(s) 208, 769
Eco130I CCWWGG 2 cut(s) 786, 2727
Eco147I AGGCCT 1 cut(s) 2594
Eco24I GRGCYC 3 cut(s) 210, 771, 2659
Eco31I GGTCTC 4 cut(s) 188, 327, 467, 503
Eco47I GGWCC 3 cut(s) 801, 1518, 1793
Eco53kI GAGCTC 2 cut(s) 208, 769
Eco81I CCTNAGG 2 cut(s) 2007, 2489
Eco88I CYCGRG 2 cut(s) 2320, 2480
EcoICRI GAGCTC 2 cut(s) 208, 769
EcoNI CCTNNNNNAGG 2 cut(s) 935, 1157
EcoO109I RGGNCCY 5 cut(s) 959, 1181, 2439, 2655, 2656
EcoRI GAATTC 2 cut(s) 484, 2285
EcoRII CCWGG 8 cut(s) 171, 1081, 1303, 1928, 2529, 2594, 2604, 2767
EcoT14I CCWWGG 2 cut(s) 786, 2727
EcoT38I GRGCYC 3 cut(s) 210, 771, 2659
ErhI CCWWGG 2 cut(s) 786, 2727
FaqI GGGAC 7 cut(s) 91, 1339, 1531, 1706, 2102, 2316, 2414
FauI CCCGC 1 cut(s) 2252
FauNDI CATATG 1 cut(s) 1880
FbaI TGATCA 2 cut(s) 678, 1852
FblI GTMKAC 2 cut(s) 575, 874
Fnu4HI GCNGC 9 cut(s) 280, 449, 2032, 2067, 2108, 2354, 2357, 2360, 2634
FriOI GRGCYC 3 cut(s) 210, 771, 2659
Fsp4HI GCNGC 9 cut(s) 280, 449, 2032, 2067, 2108, 2354, 2357, 2360, 2634
FspBI CTAG 6 cut(s) 363, 659, 963, 1185, 2679, 2733
GlaI GCGC 1 cut(s) 2568
GluI GCNGC 9 cut(s) 280, 449, 2032, 2067, 2108, 2354, 2357, 2360, 2634
GsaI CCCAGC 3 cut(s) 897, 1360, 2107
GsuI CTGGAG 6 cut(s) 137, 1104, 1326, 1794, 1951, 2513
HaeIII GGCC 9 cut(s) 146, 961, 1183, 2059, 2440, 2550, 2594, 2657, 2726
HapII CCGG 6 cut(s) 725, 1349, 1483, 2130, 2555, 2587
HgaI GACGC 1 cut(s) 2377
HhaI GCGC 1 cut(s) 2569
Hin6I GCGC 1 cut(s) 2567
HinP1I GCGC 1 cut(s) 2567
HpaII CCGG 6 cut(s) 725, 1349, 1483, 2130, 2555, 2587
HphI GGTGA 8 cut(s) 913, 1092, 1314, 1631, 1886, 1995, 2158, 2246
Hpy166II GTNNAC 5 cut(s) 33, 576, 801, 875, 1914
Hpy8I GTNNAC 5 cut(s) 33, 576, 801, 875, 1914
HpyCH4III ACNGT 5 cut(s) 867, 1252, 1446, 1900, 2651
HpyCH4IV ACGT 3 cut(s) 431, 591, 1406
HpyF10VI GCNNNNNNNGC 9 cut(s) 214, 2359, 2368, 2377, 2386, 2446, 2469, 2566, 2684
HpySE526I ACGT 3 cut(s) 431, 591, 1406
HspAI GCGC 1 cut(s) 2567
KpnI GGTACC 1 cut(s) 2497
KroI GCCGGC 1 cut(s) 1482
KroNI GCCGGC 1 cut(s) 1484
Ksp22I TGATCA 2 cut(s) 678, 1852
KspI CCGCGG 1 cut(s) 2260
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 8 cut(s) 23, 766, 788, 1085, 1307, 1604, 1813, 2557
Lsp1109I GCAGC 8 cut(s) 266, 460, 2018, 2053, 2365, 2368, 2371, 2620
LweI GCATC 8 cut(s) 570, 1023, 1072, 1245, 1294, 1501, 2376, 2599
MaeI CTAG 6 cut(s) 363, 659, 963, 1185, 2679, 2733
MaeII ACGT 3 cut(s) 431, 591, 1406
MaeIII GTNAC 7 cut(s) 12, 595, 926, 1148, 1874, 2001, 2365
MflI RGATCY 3 cut(s) 378, 643, 1978
MhlI GDGCHC 4 cut(s) 210, 771, 785, 2659
MlyI GAGTC 6 cut(s) 128, 1361, 1730, 1773, 2332, 2338
MmeI TCCRAC 4 cut(s) 318, 820, 856, 1510
MroNI GCCGGC 1 cut(s) 1482
MroXI GAANNNNTTC 3 cut(s) 855, 1947, 2411
MseI TTAA 9 cut(s) 54, 284, 348, 588, 869, 1578, 1634, 2093, 2139
MspA1I CMGCKG 1 cut(s) 2259
MspCI CTTAAG 2 cut(s) 283, 347
MspI CCGG 6 cut(s) 725, 1349, 1483, 2130, 2555, 2587
MspR9I CCNGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
MvaI CCWGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
MvnI CGCG 2 cut(s) 405, 2259
MwoI GCNNNNNNNGC 9 cut(s) 214, 2359, 2368, 2377, 2386, 2446, 2469, 2566, 2684
NaeI GCCGGC 1 cut(s) 1484
NdeI CATATG 1 cut(s) 1880
NgoMIV GCCGGC 1 cut(s) 1482
NmuCI GTSAC 3 cut(s) 1874, 2001, 2365
NspI RCATGY 1 cut(s) 2031
NspV TTCGAA 1 cut(s) 1941
PaeR7I CTCGAG 2 cut(s) 2320, 2480
PagI TCATGA 2 cut(s) 1855, 2344
PaqCI CACCTGC 1 cut(s) 2572
PceI AGGCCT 1 cut(s) 2594
PciSI GCTCTTC 1 cut(s) 198
PdiI GCCGGC 1 cut(s) 1484
PdmI GAANNNNTTC 3 cut(s) 855, 1947, 2411
PfeI GAWTC 6 cut(s) 343, 662, 851, 1271, 2740, 2786
PfoI TCCNGGA 2 cut(s) 1081, 1303
PinAI ACCGGT 1 cut(s) 2129
PkrI GCNGC 9 cut(s) 281, 450, 2033, 2068, 2109, 2355, 2358, 2361, 2635
PleI GAGTC 6 cut(s) 127, 1360, 1729, 1773, 2331, 2337
PpsI GAGTC 6 cut(s) 127, 1360, 1729, 1773, 2331, 2337
PshAI GACNNNNGTC 1 cut(s) 2519
PshBI ATTAAT 1 cut(s) 2139
Psp124BI GAGCTC 2 cut(s) 210, 771
Psp1406I AACGTT 2 cut(s) 431, 591
Psp6I CCWGG 8 cut(s) 171, 1081, 1303, 1928, 2529, 2594, 2604, 2767
PspFI CCCAGC 3 cut(s) 893, 1356, 2103
PspGI CCWGG 8 cut(s) 171, 1081, 1303, 1928, 2529, 2594, 2604, 2767
PspOMI GGGCCC 1 cut(s) 2655
PspPI GGNCC 8 cut(s) 801, 959, 1181, 1518, 1793, 2439, 2655, 2656
PspXI VCTCGAGB 1 cut(s) 2320
PstI CTGCAG 3 cut(s) 450, 496, 2053
PstNI CAGNNNCTG 1 cut(s) 1619
PsuI RGATCY 3 cut(s) 378, 643, 1978
SacI GAGCTC 2 cut(s) 210, 771
SacII CCGCGG 1 cut(s) 2260
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 9 cut(s) 54, 284, 348, 588, 869, 1578, 1634, 2093, 2139
SatI GCNGC 9 cut(s) 280, 449, 2032, 2067, 2108, 2354, 2357, 2360, 2634
Sau96I GGNCC 8 cut(s) 801, 959, 1181, 1518, 1793, 2439, 2655, 2656
ScaI AGTACT 1 cut(s) 2423
SchI GAGTC 6 cut(s) 128, 1361, 1730, 1773, 2332, 2338
ScrFI CCNGG 8 cut(s) 173, 1083, 1305, 1930, 2531, 2596, 2606, 2769
SduI GDGCHC 4 cut(s) 210, 771, 785, 2659
SfaNI GCATC 8 cut(s) 570, 1023, 1072, 1245, 1294, 1501, 2376, 2599
SfcI CTRYAG 4 cut(s) 446, 492, 1248, 2049
Sfr274I CTCGAG 2 cut(s) 2320, 2480
Sfr303I CCGCGG 1 cut(s) 2260
SfuI TTCGAA 1 cut(s) 1941
SgrBI CCGCGG 1 cut(s) 2260
SinI GGWCC 3 cut(s) 801, 1518, 1793
SlaI CTCGAG 2 cut(s) 2320, 2480
SmlI CTYRAG 8 cut(s) 283, 326, 347, 647, 1487, 2320, 2480, 2701
SmoI CTYRAG 8 cut(s) 283, 326, 347, 647, 1487, 2320, 2480, 2701
SseBI AGGCCT 1 cut(s) 2594
SsiI CCGC 4 cut(s) 403, 2108, 2257, 2259
SspI AATATT 3 cut(s) 1501, 1511, 1598
SspMI CTAG 6 cut(s) 363, 659, 963, 1185, 2679, 2733
SstI GAGCTC 2 cut(s) 210, 771
StuI AGGCCT 1 cut(s) 2594
StyD4I CCNGG 8 cut(s) 171, 1081, 1303, 1928, 2529, 2594, 2604, 2767
StyI CCWWGG 2 cut(s) 786, 2727
TaaI ACNGT 5 cut(s) 867, 1252, 1446, 1900, 2651
TaiI ACGT 3 cut(s) 434, 594, 1409
TaqI TCGA 7 cut(s) 482, 859, 1941, 2271, 2321, 2481, 2499
TaqII GACCGA 1 cut(s) 1535
TatI WGTACW 4 cut(s) 503, 816, 1453, 2421
TauI GCSGC 1 cut(s) 2110
TfiI GAWTC 6 cut(s) 343, 662, 851, 1271, 2740, 2786
Tru1I TTAA 9 cut(s) 54, 284, 348, 588, 869, 1578, 1634, 2093, 2139
Tru9I TTAA 9 cut(s) 54, 284, 348, 588, 869, 1578, 1634, 2093, 2139
TseFI GTSAC 3 cut(s) 1874, 2001, 2365
TseI GCWGC 8 cut(s) 279, 448, 2031, 2066, 2353, 2356, 2359, 2633
Tsp45I GTSAC 3 cut(s) 1874, 2001, 2365
TspDTI ATGAA 7 cut(s) 864, 969, 1008, 1071, 1191, 1230, 1379
TspGWI ACGGA 3 cut(s) 552, 786, 2189
Vha464I CTTAAG 2 cut(s) 283, 347
VpaK11BI GGWCC 3 cut(s) 801, 1518, 1793
VspI ATTAAT 1 cut(s) 2139
XagI CCTNNNNNAGG 2 cut(s) 935, 1157
XapI RAATTY 3 cut(s) 484, 2149, 2285
XceI RCATGY 1 cut(s) 2031
XcmI CCANNNNNNNNNTGG 2 cut(s) 1598, 1649
XhoI CTCGAG 2 cut(s) 2320, 2480
XmiI GTMKAC 2 cut(s) 575, 874
XmnI GAANNNNTTC 3 cut(s) 855, 1947, 2411
XspI CTAG 6 cut(s) 363, 659, 963, 1185, 2679, 2733
ZrmI AGTACT 1 cut(s) 2423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.