Rroxscaffold_3G00267900

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000408
Physical Location & Seq
Reverse (-)
61042285 .. 61043915
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267900.1

Sequence Viewer

Length: 576 bp
ATGAACTTCCATAATTTCCAGTGGGACTTGCACTGGAACGAGAAGATCCCCGGTGATGCATACAGTAATGCATCATTGGAAGAACCGGTTGATGAATGGGATATGCATGCGAGCTACTTGAAAGGGAAGCTTTTATATGGTGGTCACTTAGCCATTGTACTGACGGGACCTGAAGGATGCGGGAAAACTAGATTGGCAGAAAAGTTTTGTCAAGATGAGGAAGTCATAGATGAATTCAAGAACAATATTTTCTTCGTCCCTGTTTCGGAAAGCCCCAAATTGCAGCTTATTGTTCAAAAAATATATCCAGAGGAGGGCTACAGGAAAATTATGTTGCCAGATGAAAATGGAGTTCATGCAATTCAGTGGCTGCAAGCATTTGTTAAGCATGTAGGACATCGTCGTTCTCTGTTGGTCCTAGATGATGTCTGGCCTGGATCCGAATCCCTTCTTGATGAGTTTGATGAATTCAAAAGGTCAAATTTCAAGATTCTGGTGACATCAAGATTCGAATTTCCAAGATTTGGTTCCTCCTATTATGTAATGATGGATGATGTACTTAGGGTAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

191

Amino Acids

22.26

Weight (kDa)

5.25

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 40 - 169 1.6e-11 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000408)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11090 FvH4_5g11120 FvH4_5g11130 FvH4_5g11160 FvH4_5g11200 FvH4_5g11201 FvH4_5g11220 FvH4_5g11230 FvH4_5g11231 FvH4_5g11250
malus_domestica MD02G1021700.v1.1 MD02G1022000.v1.1 MD06G1115400.v1.1 MD06G1115800.v1.1 MD06G1116100.v1.1 MD15G1278700.v1.1
prunus_persica Prupe.5G127700_v2.0.a1 Prupe.5G127800_v2.0.a1 Prupe.5G127900_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1 Prupe.7G138400_v2.0.a1
pyrus_communis pycom06g10800 pycom06g10840 pycom14g10990 pycom15g24080 pycom15g24110 pycom15g24120
rosa_chinensis RchiOBHm_Chr2g0104561 RchiOBHm_Chr7g0186621 RchiOBHm_Chr7g0186631 RchiOBHm_Chr7g0186691
rosa_laevigata RLG00000002334 RLG00000004825 RLG00000004827 RLG00000004828 RLG00000014202
rosa_multiflora Rmu_sc0000645.1_g000001 Rmu_sc0000645.1_g000011 Rmu_sc0000645.1_g000016 Rmu_sc0001301.1_g000003 Rmu_sc0001852.1_g000020 Rmu_sc0002316.1_g000047 Rmu_sc0003419.1_g000031 Rmu_sc0003825.1_g000016 Rmu_sc0005784.1_g000004 Rmu_sc0005784.1_g000013 Rmu_sc0005784.1_g000014 Rmu_sc0007722.1_g000010 Rmu_sc0007722.1_g000018
rosa_roxburghii Rroxscaffold_3G00240400 Rroxscaffold_3G00267900 Rroxscaffold_3G00267910 Rroxscaffold_3G00267940 Rroxscaffold_3G00267990 Rroxscaffold_3G00268000
rosa_rugosa Rorug02G0116000 Rorug02G0120800 Rorug02G0120900 Rorug06G0475500 Rorug06G0475600 Rorug06G0475600 Rorug06G0475700
rosa_samantha Rh2AG164300 Rh2AG171300 Rh2AG173100 Rh2BG171500 Rh2BG178600 Rh2BG180800 Rh2DG169600 Rh2DG177300 Rh2DG179300 Rh7AG065000 Rh7AG065200 Rh7AG065300 Rh7AG321500 Rh7AG321700 Rh7AG322300 Rh7AG322400 Rh7BG081200 Rh7BG081300 Rh7BG313300 Rh7CG082400 Rh7CG082600 Rh7CG338000 Rh7CG338200 Rh7CG339000 Rh7CG339100 Rh7DG083400 Rh7DG083500 Rh7DG083700 Rh7DG319500 Rh7DG319600
rosa_wichuraiana Rw0G013250 Rw7G006930 Rw7G006940 Rw7G007150 Rw7G007180 Rw7G026920 Rw7G027300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 524
AciI CCGC 1 cut(s) 180
AclWI GGATC 3 cut(s) 40, 432, 445
AcsI RAATTY 4 cut(s) 233, 467, 481, 512
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 2 cut(s) 159, 558
AfiI CCNNNNNNNGG 3 cut(s) 265, 314, 524
AgeI ACCGGT 1 cut(s) 85
AgsI TTSAA 5 cut(s) 121, 238, 296, 472, 487
AjnI CCWGG 1 cut(s) 433
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 511, 543
AluBI AGCT 3 cut(s) 114, 130, 286
AluI AGCT 3 cut(s) 114, 130, 286
AlwI GGATC 3 cut(s) 40, 432, 445
AlwNI CAGNNNCTG 1 cut(s) 370
AoxI GGCC 1 cut(s) 431
ApeKI GCWGC 2 cut(s) 283, 370
ApoI RAATTY 4 cut(s) 233, 467, 481, 512
AsiGI ACCGGT 1 cut(s) 85
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 2 cut(s) 167, 415
AsuC2I CCSGG 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 65, 508
AsuII TTCGAA 1 cut(s) 510
AvaII GGWCC 2 cut(s) 167, 415
BamHI GGATCC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 295, 357
BccI CCATC 1 cut(s) 541
BciT130I CCWGG 1 cut(s) 435
BcnI CCSGG 1 cut(s) 51
BfaI CTAG 2 cut(s) 189, 419
BfmI CTRYAG 1 cut(s) 319
BisI GCNGC 2 cut(s) 284, 371
BlsI GCNGC 2 cut(s) 285, 372
Bme1390I CCNGG 2 cut(s) 51, 435
Bme18I GGWCC 2 cut(s) 167, 415
BmgT120I GGNCC 2 cut(s) 167, 415
BmiI GGNNCC 3 cut(s) 168, 439, 529
BmrFI CCNGG 2 cut(s) 51, 435
BmsI GCATC 3 cut(s) 46, 80, 167
Bpu14I TTCGAA 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 442
BsaJI CCNNGG 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 85
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 314, 524
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 2 cut(s) 19, 38
Bse8I GATNNNNATC 1 cut(s) 442
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 2 cut(s) 182, 556
BseJI GATNNNNATC 1 cut(s) 442
BseLI CCNNNNNNNGG 3 cut(s) 265, 314, 524
BseNI ACTGG 2 cut(s) 19, 38
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 2 cut(s) 295, 357
BshFI GGCC 1 cut(s) 433
BshTI ACCGGT 1 cut(s) 85
BsiSI CCGG 2 cut(s) 51, 86
BslFI GGGAC 3 cut(s) 38, 180, 242
BslI CCNNNNNNNGG 3 cut(s) 265, 314, 524
BsmFI GGGAC 3 cut(s) 38, 180, 242
BsnI GGCC 1 cut(s) 433
Bsp119I TTCGAA 1 cut(s) 510
Bsp143I GATC 2 cut(s) 45, 437
BspACI CCGC 1 cut(s) 180
BspANI GGCC 1 cut(s) 433
BspLI GGNNCC 3 cut(s) 168, 439, 529
BspPI GGATC 3 cut(s) 40, 432, 445
BspT104I TTCGAA 1 cut(s) 510
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 2 cut(s) 19, 38
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 45, 437
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 510
BstC8I GCNNGC 3 cut(s) 108, 112, 375
BstDEI CTNAG 2 cut(s) 148, 560
BstF5I GGATG 2 cut(s) 182, 556
BstKTI GATC 2 cut(s) 48, 440
BstMBI GATC 2 cut(s) 45, 437
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 2 cut(s) 110, 392
BstSCI CCNGG 2 cut(s) 49, 433
BstSFI CTRYAG 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 295, 357
BstX2I RGATCY 2 cut(s) 45, 437
BstYI RGATCY 2 cut(s) 45, 437
BsuRI GGCC 1 cut(s) 433
BtsCI GGATG 2 cut(s) 182, 556
BtsIMutI CAGTG 3 cut(s) 26, 31, 371
Cac8I GCNNGC 3 cut(s) 108, 112, 375
CaiI CAGNNNCTG 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 2 cut(s) 167, 415
Csp6I GTAC 2 cut(s) 158, 557
CspAI ACCGGT 1 cut(s) 85
CviAII CATG 3 cut(s) 107, 356, 389
CviJI RGCY 8 cut(s) 114, 130, 152, 273, 286, 318, 370, 433
CviKI_1 RGCY 8 cut(s) 114, 130, 152, 273, 286, 318, 370, 433
CviQI GTAC 2 cut(s) 158, 557
DdeI CTNAG 2 cut(s) 148, 560
DpnI GATC 2 cut(s) 47, 439
DpnII GATC 2 cut(s) 45, 437
Eco47I GGWCC 2 cut(s) 167, 415
Eco57I CTGAAG 1 cut(s) 192
EcoO109I RGGNCCY 1 cut(s) 167
EcoRI GAATTC 2 cut(s) 233, 467
EcoRII CCWGG 1 cut(s) 433
EcoT22I ATGCAT 3 cut(s) 61, 73, 108
FaeI CATG 3 cut(s) 110, 359, 392
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 3 cut(s) 38, 180, 242
FatI CATG 3 cut(s) 106, 355, 388
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 2 cut(s) 284, 371
FokI GGATG 2 cut(s) 189, 563
Fsp4HI GCNGC 2 cut(s) 284, 371
FspBI CTAG 2 cut(s) 189, 419
GluI GCNGC 2 cut(s) 284, 371
HaeIII GGCC 1 cut(s) 433
HapII CCGG 2 cut(s) 51, 86
Hin1II CATG 3 cut(s) 110, 359, 392
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 3 cut(s) 443, 490, 507
HpaII CCGG 2 cut(s) 51, 86
HphI GGTGA 2 cut(s) 65, 508
Hpy188I TCNGA 2 cut(s) 268, 442
Hpy188III TCNNGA 6 cut(s) 212, 238, 308, 452, 487, 504
Hpy99I CGWCG 1 cut(s) 405
HpyAV CCTTC 2 cut(s) 167, 458
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4V TGCA 7 cut(s) 31, 59, 71, 106, 283, 359, 373
HpyF3I CTNAG 2 cut(s) 148, 560
Hsp92II CATG 3 cut(s) 110, 359, 392
Kzo9I GATC 2 cut(s) 45, 437
Lsp1109I GCAGC 2 cut(s) 295, 357
LweI GCATC 3 cut(s) 46, 80, 167
MaeI CTAG 2 cut(s) 189, 419
MaeIII GTNAC 2 cut(s) 143, 496
MalI GATC 2 cut(s) 47, 439
MboI GATC 2 cut(s) 45, 437
MboII GAAGA 3 cut(s) 55, 92, 244
MflI RGATCY 2 cut(s) 45, 437
MluCI AATT 8 cut(s) 13, 233, 278, 327, 360, 467, 481, 512
MnlI CCTC 4 cut(s) 211, 304, 307, 541
Mph1103I ATGCAT 3 cut(s) 61, 73, 108
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 1 cut(s) 384
MspI CCGG 2 cut(s) 51, 86
MspR9I CCNGG 2 cut(s) 51, 435
MvaI CCWGG 1 cut(s) 435
NciI CCSGG 1 cut(s) 51
NdeII GATC 2 cut(s) 45, 437
NlaIII CATG 3 cut(s) 110, 359, 392
NlaIV GGNNCC 3 cut(s) 168, 439, 529
NmuCI GTSAC 2 cut(s) 143, 496
NsiI ATGCAT 3 cut(s) 61, 73, 108
NspI RCATGY 2 cut(s) 110, 392
NspV TTCGAA 1 cut(s) 510
PaeI GCATGC 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 3 cut(s) 443, 490, 507
PflFI GACNNNGTC 1 cut(s) 399
PflMI CCANNNNNTGG 1 cut(s) 524
PinAI ACCGGT 1 cut(s) 85
PkrI GCNGC 2 cut(s) 285, 372
PpuMI RGGWCCY 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 168, 439, 529
PspPI GGNCC 2 cut(s) 167, 415
PspPPI RGGWCCY 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 370
PsuI RGATCY 2 cut(s) 45, 437
PsyI GACNNNGTC 1 cut(s) 399
RsaI GTAC 2 cut(s) 159, 558
RsaNI GTAC 2 cut(s) 158, 557
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 284, 371
Sau3AI GATC 2 cut(s) 45, 437
Sau96I GGNCC 2 cut(s) 167, 415
ScrFI CCNGG 2 cut(s) 51, 435
SetI ASST 6 cut(s) 116, 132, 172, 288, 479, 571
SfaNI GCATC 3 cut(s) 46, 80, 167
SfcI CTRYAG 1 cut(s) 319
SfuI TTCGAA 1 cut(s) 510
SinI GGWCC 2 cut(s) 167, 415
SphI GCATGC 1 cut(s) 110
Sse9I AATT 8 cut(s) 13, 233, 278, 327, 360, 467, 481, 512
SsiI CCGC 1 cut(s) 180
SspI AATATT 1 cut(s) 247
SspMI CTAG 2 cut(s) 189, 419
StyD4I CCNGG 2 cut(s) 49, 433
TaaI ACNGT 1 cut(s) 65
TaqI TCGA 1 cut(s) 510
TasI AATT 8 cut(s) 13, 233, 278, 327, 360, 467, 481, 512
TatI WGTACW 2 cut(s) 157, 556
TfiI GAWTC 3 cut(s) 443, 490, 507
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 3 cut(s) 26, 38, 371
TseFI GTSAC 2 cut(s) 143, 496
TseI GCWGC 2 cut(s) 283, 370
Tsp45I GTSAC 2 cut(s) 143, 496
TspDTI ATGAA 6 cut(s) 17, 108, 246, 344, 357, 480
TspRI CASTG 3 cut(s) 26, 38, 371
Tth111I GACNNNGTC 1 cut(s) 399
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 167, 415
XapI RAATTY 4 cut(s) 233, 467, 481, 512
XceI RCATGY 2 cut(s) 110, 392
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 2 cut(s) 189, 419
Zsp2I ATGCAT 3 cut(s) 61, 73, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.