Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 705 |
| AatII | GACGTC | 1 cut(s) | 473 |
| Acc36I | ACCTGC | 3 cut(s) | 436, 725, 739 |
| AccB1I | GGYRCC | 1 cut(s) | 52 |
| AccBSI | CCGCTC | 1 cut(s) | 196 |
| AciI | CCGC | 5 cut(s) | 78, 194, 788, 968, 1126 |
| AclWI | GGATC | 2 cut(s) | 965, 978 |
| AcsI | RAATTY | 2 cut(s) | 548, 642 |
| AcyI | GRCGYC | 1 cut(s) | 470 |
| AfaI | GTAC | 2 cut(s) | 377, 435 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 283, 424, 677 |
| AflII | CTTAAG | 2 cut(s) | 794, 842 |
| AgsI | TTSAA | 3 cut(s) | 86, 149, 939 |
| AjnI | CCWGG | 1 cut(s) | 930 |
| AluBI | AGCT | 4 cut(s) | 328, 366, 686, 914 |
| AluI | AGCT | 4 cut(s) | 328, 366, 686, 914 |
| Alw21I | GWGCWC | 2 cut(s) | 344, 688 |
| Alw26I | GTCTC | 3 cut(s) | 415, 461, 971 |
| AlwI | GGATC | 2 cut(s) | 965, 978 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 116, 623 |
| Ama87I | CYCGRG | 1 cut(s) | 974 |
| AoxI | GGCC | 1 cut(s) | 9 |
| ApeKI | GCWGC | 2 cut(s) | 23, 26 |
| ApoI | RAATTY | 2 cut(s) | 548, 642 |
| AspLEI | GCGC | 1 cut(s) | 779 |
| AspS9I | GGNCC | 2 cut(s) | 668, 1095 |
| AsuHPI | GGTGA | 1 cut(s) | 544 |
| AvaI | CYCGRG | 1 cut(s) | 974 |
| AvaII | GGWCC | 2 cut(s) | 668, 1095 |
| BamHI | GGATCC | 1 cut(s) | 970 |
| BanI | GGYRCC | 1 cut(s) | 52 |
| BanII | GRGCYC | 1 cut(s) | 688 |
| Bbv12I | GWGCWC | 2 cut(s) | 344, 688 |
| BbvCI | CCTCAGC | 1 cut(s) | 915 |
| BbvI | GCAGC | 2 cut(s) | 10, 13 |
| BccI | CCATC | 4 cut(s) | 43, 241, 523, 750 |
| BciT130I | CCWGG | 1 cut(s) | 932 |
| BclI | TGATCA | 2 cut(s) | 534, 628 |
| BcoDI | GTCTC | 3 cut(s) | 415, 461, 971 |
| BfaI | CTAG | 2 cut(s) | 122, 750 |
| BfmI | CTRYAG | 3 cut(s) | 27, 722, 732 |
| BfoI | RGCGCY | 1 cut(s) | 780 |
| BfrI | CTTAAG | 2 cut(s) | 794, 842 |
| BfuAI | ACCTGC | 3 cut(s) | 436, 725, 739 |
| BglII | AGATCT | 1 cut(s) | 202 |
| BisI | GCNGC | 3 cut(s) | 24, 27, 79 |
| BlsI | GCNGC | 3 cut(s) | 25, 28, 80 |
| Bme1390I | CCNGG | 1 cut(s) | 932 |
| Bme18I | GGWCC | 2 cut(s) | 668, 1095 |
| BmeT110I | CYCGRG | 1 cut(s) | 974 |
| BmgT120I | GGNCC | 2 cut(s) | 668, 1095 |
| BmiI | GGNNCC | 3 cut(s) | 54, 670, 972 |
| BmrFI | CCNGG | 1 cut(s) | 932 |
| BmsI | GCATC | 3 cut(s) | 101, 129, 280 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 312, 344 |
| Bpu10I | CCTNAGC | 1 cut(s) | 915 |
| BsaBI | GATNNNNATC | 1 cut(s) | 533 |
| BsaHI | GRCGYC | 1 cut(s) | 470 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 283, 424, 677 |
| Bse1I | ACTGG | 2 cut(s) | 562, 1048 |
| Bse8I | GATNNNNATC | 1 cut(s) | 533 |
| BseBI | CCWGG | 1 cut(s) | 932 |
| BseGI | GGATG | 4 cut(s) | 144, 412, 463, 925 |
| BseJI | GATNNNNATC | 1 cut(s) | 533 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 283, 424, 677 |
| BseMII | CTCAG | 5 cut(s) | 428, 781, 820, 868, 906 |
| BseNI | ACTGG | 2 cut(s) | 562, 1048 |
| BseRI | GAGGAG | 3 cut(s) | 48, 257, 696 |
| BseXI | GCAGC | 2 cut(s) | 10, 13 |
| Bsh1285I | CGRYCG | 1 cut(s) | 593 |
| BshFI | GGCC | 1 cut(s) | 11 |
| BshNI | GGYRCC | 1 cut(s) | 52 |
| BsiEI | CGRYCG | 1 cut(s) | 593 |
| BsiHKAI | GWGCWC | 2 cut(s) | 344, 688 |
| BsiHKCI | CYCGRG | 1 cut(s) | 974 |
| BsiSI | CCGG | 1 cut(s) | 418 |
| BslFI | GGGAC | 4 cut(s) | 121, 127, 812, 860 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 283, 424, 677 |
| BsmAI | GTCTC | 3 cut(s) | 415, 461, 971 |
| BsmBI | CGTCTC | 1 cut(s) | 461 |
| BsmFI | GGGAC | 4 cut(s) | 121, 127, 812, 860 |
| BsmI | GAATGC | 2 cut(s) | 81, 611 |
| BsnI | GGCC | 1 cut(s) | 11 |
| BsoBI | CYCGRG | 1 cut(s) | 974 |
| Bsp1286I | GDGCHC | 2 cut(s) | 344, 688 |
| Bsp143I | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| BspACI | CCGC | 5 cut(s) | 78, 194, 788, 968, 1126 |
| BspANI | GGCC | 1 cut(s) | 11 |
| BspCNI | CTCAG | 5 cut(s) | 429, 780, 819, 867, 907 |
| BspLI | GGNNCC | 3 cut(s) | 54, 670, 972 |
| BspMAI | CTGCAG | 3 cut(s) | 31, 726, 736 |
| BspMI | ACCTGC | 3 cut(s) | 436, 725, 739 |
| BspPI | GGATC | 2 cut(s) | 965, 978 |
| BspT107I | GGYRCC | 1 cut(s) | 52 |
| BspTI | CTTAAG | 2 cut(s) | 794, 842 |
| BsrBI | CCGCTC | 1 cut(s) | 196 |
| BsrI | ACTGG | 2 cut(s) | 562, 1048 |
| BssMI | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| BssNI | GRCGYC | 1 cut(s) | 470 |
| Bst2UI | CCWGG | 1 cut(s) | 932 |
| Bst4CI | ACNGT | 1 cut(s) | 619 |
| Bst6I | CTCTTC | 1 cut(s) | 492 |
| BstACI | GRCGYC | 1 cut(s) | 470 |
| BstAFI | CTTAAG | 2 cut(s) | 794, 842 |
| BstC8I | GCNNGC | 1 cut(s) | 364 |
| BstDEI | CTNAG | 7 cut(s) | 345, 437, 767, 806, 854, 915, 1105 |
| BstF5I | GGATG | 4 cut(s) | 144, 412, 463, 925 |
| BstH2I | RGCGCY | 1 cut(s) | 780 |
| BstHHI | GCGC | 1 cut(s) | 779 |
| BstKTI | GATC | 5 cut(s) | 205, 537, 593, 631, 973 |
| BstMAI | GTCTC | 3 cut(s) | 415, 461, 971 |
| BstMBI | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| BstMCI | CGRYCG | 1 cut(s) | 593 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 125, 683, 785 |
| BstNI | CCWGG | 1 cut(s) | 932 |
| BstNSI | RCATGY | 1 cut(s) | 968 |
| BstSCI | CCNGG | 1 cut(s) | 930 |
| BstSFI | CTRYAG | 3 cut(s) | 27, 722, 732 |
| BstV1I | GCAGC | 2 cut(s) | 10, 13 |
| BstX2I | RGATCY | 2 cut(s) | 202, 970 |
| BstYI | RGATCY | 2 cut(s) | 202, 970 |
| BsuRI | GGCC | 1 cut(s) | 11 |
| BtsCI | GGATG | 4 cut(s) | 144, 412, 463, 925 |
| BtsI | GCAGTG | 1 cut(s) | 309 |
| BtsIMutI | CAGTG | 3 cut(s) | 309, 555, 624 |
| BveI | ACCTGC | 3 cut(s) | 436, 725, 739 |
| Cac8I | GCNNGC | 1 cut(s) | 364 |
| CaiI | CAGNNNCTG | 2 cut(s) | 116, 623 |
| CfoI | GCGC | 1 cut(s) | 779 |
| Cfr13I | GGNCC | 2 cut(s) | 668, 1095 |
| Csp6I | GTAC | 2 cut(s) | 376, 434 |
| CviAII | CATG | 4 cut(s) | 821, 869, 965, 989 |
| CviQI | GTAC | 2 cut(s) | 376, 434 |
| DdeI | CTNAG | 7 cut(s) | 345, 437, 767, 806, 854, 915, 1105 |
| DpnI | GATC | 5 cut(s) | 204, 536, 592, 630, 972 |
| DpnII | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| Eam1104I | CTCTTC | 1 cut(s) | 492 |
| EarI | CTCTTC | 1 cut(s) | 492 |
| Ecl136II | GAGCTC | 1 cut(s) | 686 |
| Eco147I | AGGCCT | 1 cut(s) | 11 |
| Eco24I | GRGCYC | 1 cut(s) | 688 |
| Eco47I | GGWCC | 2 cut(s) | 668, 1095 |
| Eco53kI | GAGCTC | 1 cut(s) | 686 |
| Eco88I | CYCGRG | 1 cut(s) | 974 |
| EcoICRI | GAGCTC | 1 cut(s) | 686 |
| EcoRII | CCWGG | 1 cut(s) | 930 |
| EcoT38I | GRGCYC | 1 cut(s) | 688 |
| Esp3I | CGTCTC | 1 cut(s) | 461 |
| FaeI | CATG | 4 cut(s) | 824, 872, 968, 992 |
| FaqI | GGGAC | 4 cut(s) | 121, 127, 812, 860 |
| FatI | CATG | 4 cut(s) | 820, 868, 964, 988 |
| FauNDI | CATATG | 2 cut(s) | 832, 880 |
| FbaI | TGATCA | 2 cut(s) | 534, 628 |
| Fnu4HI | GCNGC | 3 cut(s) | 24, 27, 79 |
| FokI | GGATG | 4 cut(s) | 151, 419, 470, 932 |
| FriOI | GRGCYC | 1 cut(s) | 688 |
| Fsp4HI | GCNGC | 3 cut(s) | 24, 27, 79 |
| FspBI | CTAG | 2 cut(s) | 122, 750 |
| GlaI | GCGC | 1 cut(s) | 778 |
| GluI | GCNGC | 3 cut(s) | 24, 27, 79 |
| HaeII | RGCGCY | 1 cut(s) | 780 |
| HaeIII | GGCC | 1 cut(s) | 11 |
| HapII | CCGG | 1 cut(s) | 418 |
| HhaI | GCGC | 1 cut(s) | 779 |
| Hin1I | GRCGYC | 1 cut(s) | 470 |
| Hin1II | CATG | 4 cut(s) | 824, 872, 968, 992 |
| Hin6I | GCGC | 1 cut(s) | 777 |
| HinP1I | GCGC | 1 cut(s) | 777 |
| HincII | GTYRAC | 1 cut(s) | 962 |
| HindII | GTYRAC | 1 cut(s) | 962 |
| HinfI | GANTC | 4 cut(s) | 58, 221, 1005, 1058 |
| HpaII | CCGG | 1 cut(s) | 418 |
| HphI | GGTGA | 1 cut(s) | 544 |
| Hpy166II | GTNNAC | 2 cut(s) | 434, 962 |
| Hpy188I | TCNGA | 7 cut(s) | 668, 770, 809, 857, 1057, 1078, 1087 |
| Hpy188III | TCNNGA | 2 cut(s) | 974, 1093 |
| Hpy8I | GTNNAC | 2 cut(s) | 434, 962 |
| Hpy99I | CGWCG | 1 cut(s) | 475 |
| HpyAV | CCTTC | 4 cut(s) | 62, 160, 487, 700 |
| HpyCH4III | ACNGT | 1 cut(s) | 619 |
| HpyCH4IV | ACGT | 1 cut(s) | 470 |
| HpyCH4V | TGCA | 7 cut(s) | 29, 271, 302, 336, 566, 724, 734 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 125, 683, 785 |
| HpyF3I | CTNAG | 7 cut(s) | 345, 437, 767, 806, 854, 915, 1105 |
| HpySE526I | ACGT | 1 cut(s) | 470 |
| Hsp92I | GRCGYC | 1 cut(s) | 470 |
| Hsp92II | CATG | 4 cut(s) | 824, 872, 968, 992 |
| HspAI | GCGC | 1 cut(s) | 777 |
| Ksp22I | TGATCA | 2 cut(s) | 534, 628 |
| Kzo9I | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| LmnI | GCTCC | 4 cut(s) | 125, 201, 683, 691 |
| Lsp1109I | GCAGC | 2 cut(s) | 10, 13 |
| LweI | GCATC | 3 cut(s) | 101, 129, 280 |
| MaeI | CTAG | 2 cut(s) | 122, 750 |
| MaeII | ACGT | 1 cut(s) | 470 |
| MalI | GATC | 5 cut(s) | 204, 536, 592, 630, 972 |
| MbiI | CCGCTC | 1 cut(s) | 196 |
| MboI | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| MboII | GAAGA | 2 cut(s) | 17, 509 |
| MflI | RGATCY | 2 cut(s) | 202, 970 |
| MhlI | GDGCHC | 2 cut(s) | 344, 688 |
| MluCI | AATT | 3 cut(s) | 548, 642, 950 |
| MlyI | GAGTC | 1 cut(s) | 999 |
| MmeI | TCCRAC | 1 cut(s) | 932 |
| MseI | TTAA | 6 cut(s) | 186, 227, 515, 654, 795, 843 |
| MslI | CAYNNNNRTG | 1 cut(s) | 86 |
| MspA1I | CMGCKG | 1 cut(s) | 914 |
| MspCI | CTTAAG | 2 cut(s) | 794, 842 |
| MspI | CCGG | 1 cut(s) | 418 |
| MspR9I | CCNGG | 1 cut(s) | 932 |
| Mva1269I | GAATGC | 2 cut(s) | 81, 611 |
| MvaI | CCWGG | 1 cut(s) | 932 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 125, 683, 785 |
| NdeI | CATATG | 2 cut(s) | 832, 880 |
| NdeII | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| NlaIII | CATG | 4 cut(s) | 824, 872, 968, 992 |
| NlaIV | GGNNCC | 3 cut(s) | 54, 670, 972 |
| NspI | RCATGY | 1 cut(s) | 968 |
| PceI | AGGCCT | 1 cut(s) | 11 |
| PctI | GAATGC | 2 cut(s) | 81, 611 |
| PfeI | GAWTC | 3 cut(s) | 58, 221, 1058 |
| PkrI | GCNGC | 3 cut(s) | 25, 28, 80 |
| Ple19I | CGATCG | 1 cut(s) | 593 |
| PleI | GAGTC | 1 cut(s) | 999 |
| PpsI | GAGTC | 1 cut(s) | 999 |
| PsiI | TTATAA | 1 cut(s) | 705 |
| Psp124BI | GAGCTC | 1 cut(s) | 688 |
| Psp6I | CCWGG | 1 cut(s) | 930 |
| PspGI | CCWGG | 1 cut(s) | 930 |
| PspN4I | GGNNCC | 3 cut(s) | 54, 670, 972 |
| PspPI | GGNCC | 2 cut(s) | 668, 1095 |
| PstI | CTGCAG | 3 cut(s) | 31, 726, 736 |
| PstNI | CAGNNNCTG | 2 cut(s) | 116, 623 |
| PsuI | RGATCY | 2 cut(s) | 202, 970 |
| PvuI | CGATCG | 1 cut(s) | 593 |
| PvuII | CAGCTG | 1 cut(s) | 914 |
| RsaI | GTAC | 2 cut(s) | 377, 435 |
| RsaNI | GTAC | 2 cut(s) | 376, 434 |
| RseI | CAYNNNNRTG | 1 cut(s) | 86 |
| SacI | GAGCTC | 1 cut(s) | 688 |
| SaqAI | TTAA | 6 cut(s) | 186, 227, 515, 654, 795, 843 |
| SatI | GCNGC | 3 cut(s) | 24, 27, 79 |
| Sau3AI | GATC | 5 cut(s) | 202, 534, 590, 628, 970 |
| Sau96I | GGNCC | 2 cut(s) | 668, 1095 |
| SbfI | CCTGCAGG | 1 cut(s) | 736 |
| SchI | GAGTC | 1 cut(s) | 999 |
| ScrFI | CCNGG | 1 cut(s) | 932 |
| SdaI | CCTGCAGG | 1 cut(s) | 736 |
| SduI | GDGCHC | 2 cut(s) | 344, 688 |
| SfaNI | GCATC | 3 cut(s) | 101, 129, 280 |
| SfcI | CTRYAG | 3 cut(s) | 27, 722, 732 |
| SinI | GGWCC | 2 cut(s) | 668, 1095 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 86 |
| SmlI | CTYRAG | 2 cut(s) | 794, 842 |
| SmoI | CTYRAG | 2 cut(s) | 794, 842 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 736 |
| Sse9I | AATT | 3 cut(s) | 548, 642, 950 |
| SseBI | AGGCCT | 1 cut(s) | 11 |
| SsiI | CCGC | 5 cut(s) | 78, 194, 788, 968, 1126 |
| SspI | AATATT | 1 cut(s) | 132 |
| SspMI | CTAG | 2 cut(s) | 122, 750 |
| SstI | GAGCTC | 1 cut(s) | 688 |
| StuI | AGGCCT | 1 cut(s) | 11 |
| StyD4I | CCNGG | 1 cut(s) | 930 |
| TaaI | ACNGT | 1 cut(s) | 619 |
| TaiI | ACGT | 1 cut(s) | 473 |
| TaqI | TCGA | 2 cut(s) | 240, 589 |
| TasI | AATT | 3 cut(s) | 548, 642, 950 |
| TatI | WGTACW | 1 cut(s) | 433 |
| TauI | GCSGC | 1 cut(s) | 81 |
| TfiI | GAWTC | 3 cut(s) | 58, 221, 1058 |
| Tru1I | TTAA | 6 cut(s) | 186, 227, 515, 654, 795, 843 |
| Tru9I | TTAA | 6 cut(s) | 186, 227, 515, 654, 795, 843 |
| TscAI | CASTG | 3 cut(s) | 309, 562, 624 |
| TseI | GCWGC | 2 cut(s) | 23, 26 |
| TspDTI | ATGAA | 8 cut(s) | 81, 560, 627, 682, 827, 849, 875, 897 |
| TspRI | CASTG | 3 cut(s) | 309, 562, 624 |
| Vha464I | CTTAAG | 2 cut(s) | 794, 842 |
| VpaK11BI | GGWCC | 2 cut(s) | 668, 1095 |
| XapI | RAATTY | 2 cut(s) | 548, 642 |
| XceI | RCATGY | 1 cut(s) | 968 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 885, 1040 |
| XspI | CTAG | 2 cut(s) | 122, 750 |
| ZraI | GACGTC | 1 cut(s) | 471 |