FvH4_6g16970

Threonine dehydratase biosynthetic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000499
Physical Location & Seq
Reverse (-)
10835753 .. 10840485
4733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16970.t1

Sequence Viewer

Length: 1281 bp
ATGAGTGACACAACGCAGCCGAATCACAATGGAGTTAATGCAACCGATTGTTCTGTGTTAAATGCAACCGATTTTTCTGTGGTAAATGCAACCTATTTTTCTGTGGTCTCTGCATATACTATTGGATATGGAGAGTTCCATTTCTCAGCCACTGCTGGGAAGTTGTTTGCCTCAGTGTGGCTTCCTTTAGGGACTGCAGTTGTTAGTAGTGCTACATCTTACCTATGTGGACACAGGCATTGGATTTGTGCGGTTTGCATCTGGAACGGTAAGAAGACAAGGATAGTTCCAGGGCAGGAATTTGATAAAGGTACTCTTGTCGAAGCTAACCCAACATTGAGGAAGCATTGCCTTGAACACACTAATGTAGAAAATTCCCCAAAGTTTCTAAGTGGCATACTCTCGTCAAGAATTTATGAGTGCAAGTTTGCTCAAAGACAGGACCTAGAACCAGTCCATACATGTTCTCGTGAGTTCAGTTCAACCCCAGAAGAAGTACAGATATTACTCAAAAGAGAAGACTGCTGCTCGAAGGAACTTGGTTCATCCTACAGATGGAGAGGGACCTATAACTTCATGGCAAATGCACTGAATGAAATGCAGAGGAGGGCATTTATATGCACAGTTGGAAAACATGCAGTGACAGTAGCATGTGTAGCACAACTCTTGAATTCAGAAGCCAGGGTTGCGATCCCAGTTGATTATTTTTCTGCTGAACAGAATGATAGGGAGAAAATTAAAGTTGTTTTAGATAAAAAGCAATGCTATAAGGCTTTCAAAGGGACTTGCATTCAAGAATCCGATGGTTATGCCAAAAAGGTTGCTACAAATCAAAACATAATCTATGTACCTTCACGCGACCACACCGACATTATTACAGGGTATGGAACCATTGGGGTGGAGATAATCACTCAAATGGTTGGAGATGGAAAAATTGATAATCTTCATGCAATTTTCGTTCCTGTCGGTGATGGGAACCGTATTGCTGGAATTGCCGCATACGTAAAGAGGGTTTTCCCAAAGGTAAAAGTTTTTGGTGTGGAGGAGCGCACTAGAAGTGTAATGGCATGGTCATTCCATAAAGGCAAGAGAGTTCTACTTGAGAAACAAAATGGGGAAGTACTAGTAGAAGCCATTGGTGAAGAATGCTTCTCCATTTGCGCTGAGTTTCTTGATGGTATAATCATTGTAGATGAGCATGTTATATCCACAGCAAACGAGTATATCAAGAGGGACACGATAAAGAAAGTTAGTGCCCTATGCATTGCTGGGGTGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00290 ko01100 ko01110 ko01130 ko01200 ko01230 map00260 map00290 map01100 map01110 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP Thr_dehydrat_C

Protein Analysis

427

Amino Acids

47.24

Weight (kDa)

7.83

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 24 - 74 3e-09 Ion channel
PALP PF00291 165 - 405 1e-27 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02510
fragaria_vesca FvH4_6g16970 FvH4_6g16980 FvH4_7g01220 FvH4_7g01220 FvH4_7g01220 FvH4_7g01240
malus_domestica MD05G1161500.v1.1
pyrus_communis pycom05g15160
rosa_chinensis RchiOBHm_Chr1g0316711 RchiOBHm_Chr1g0316801 RchiOBHm_Chr1g0316871 RchiOBHm_Chr1g0316881 RchiOBHm_Chr1g0316951 RchiOBHm_Chr3g0470991 RchiOBHm_Chr4g0402171
rosa_laevigata RLG00000001679 RLG00000001680 RLG00000007591 RLG00000009108 RLG00000024215 RLG00000024221 RLG00000030636 RLG00000030640 RLG00000030642 RLG00000030648 RLG00000030651 RLG00000030658 RLG00000030667
rosa_multiflora Rmu_sc0000839.1_g000010 Rmu_sc0002667.1_g000003 Rmu_sc0003008.1_g000023 Rmu_sc0003008.1_g000047 Rmu_sc0005604.1_g000001 Rmu_sc0005939.1_g000001 Rmu_sc0006790.1_g000011 Rmu_sc0007472.1_g000004 Rmu_sc0017515.1_g000001 Rmu_sc0019753.1_g000003 Rmu_sc0020172.1_g000001 Rmu_sc0023783.1_g000001
rosa_roxburghii Rroxscaffold_4G00330760 Rroxscaffold_4G00330800 Rroxscaffold_4G00330810 Rroxscaffold_4G00330950
rosa_rugosa Rorug01G0011200 Rorug01G0011500 Rorug01G0011600 Rorug01G0012300 Rorug01G0012300 Rorug01G0012400 Rorug01G0012500 Rorug01G0012600 Rorug01G0012600 Rorug01G0013400 Rorug01G0013400 Rorug01G0013500
rosa_samantha Rh1AG021100 Rh1AG022800 Rh1AG023300 Rh1AG023500 Rh1AG024000 Rh1AG024300 Rh1BG016000 Rh1BG016300 Rh1BG021300 Rh1BG021500 Rh1CG020300 Rh1CG022500 Rh1DG016300 Rh1DG016400 Rh1DG017300 Rh1DG017400 Rh1DG017700 Rh1DG017800 Rh1DG018200 Rh1DG019100 Rh1DG019900 Rh1DG020200 Rh1DG020800 Rh1DG021300 Rh1DG021400 Rh1DG021800 Rh3DG186400
rosa_wichuraiana Rw1G001370 Rw1G001480 Rw1G001650 Rw1G001680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 858
AciI CCGC 2 cut(s) 251, 996
AclWI GGATC 1 cut(s) 685
AcsI RAATTY 4 cut(s) 299, 373, 411, 670
AfaI GTAC 4 cut(s) 313, 498, 849, 1122
AfiI CCNNNNNNNGG 3 cut(s) 156, 177, 555
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 5 cut(s) 356, 483, 670, 778, 794
AhlI ACTAGT 1 cut(s) 1123
AjnI CCWGG 2 cut(s) 289, 680
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 1 cut(s) 685
AlwNI CAGNNNCTG 1 cut(s) 152
ApeKI GCWGC 2 cut(s) 16, 525
ApoI RAATTY 4 cut(s) 299, 373, 411, 670
AspLEI GCGC 2 cut(s) 1050, 1163
AspS9I GGNCC 2 cut(s) 442, 564
AsuHPI GGTGA 2 cut(s) 980, 1151
AvaII GGWCC 2 cut(s) 442, 564
BaeGI GKGCMC 1 cut(s) 1258
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BauI CACGAG 1 cut(s) 468
BbsI GAAGAC 2 cut(s) 281, 525
BbvI GCAGC 2 cut(s) 28, 512
BccI CCATC 5 cut(s) 549, 797, 920, 965, 1169
BcgI CGANNNNNNTGC 2 cut(s) 791, 825
BciT130I CCWGG 2 cut(s) 291, 682
BcoDI GTCTC 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 1123
BfaI CTAG 3 cut(s) 446, 1053, 1124
BfmI CTRYAG 2 cut(s) 195, 550
BisI GCNGC 3 cut(s) 17, 526, 996
BlsI GCNGC 3 cut(s) 18, 527, 997
BmcAI AGTACT 1 cut(s) 1122
Bme1390I CCNGG 2 cut(s) 291, 682
Bme18I GGWCC 2 cut(s) 442, 564
BmgT120I GGNCC 2 cut(s) 442, 564
BmiI GGNNCC 3 cut(s) 565, 889, 977
BmrFI CCNGG 2 cut(s) 291, 682
BmrI ACTGGG 1 cut(s) 689
BmsI GCATC 1 cut(s) 267
BmuI ACTGGG 1 cut(s) 689
BpiI GAAGAC 2 cut(s) 281, 525
BpuEI CTTGAG 1 cut(s) 1121
BsaAI YACGTR 1 cut(s) 1003
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 290, 681
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 177, 555
Bse1I ACTGG 2 cut(s) 452, 695
Bse3DI GCAATG 3 cut(s) 346, 767, 1263
BseBI CCWGG 2 cut(s) 291, 682
BseDI CCNNGG 2 cut(s) 290, 681
BseGI GGATG 1 cut(s) 545
BseLI CCNNNNNNNGG 3 cut(s) 156, 177, 555
BseMI GCAATG 3 cut(s) 346, 767, 1263
BseMII CTCAG 3 cut(s) 159, 186, 1155
BseNI ACTGG 2 cut(s) 452, 695
BseRI GAGGAG 2 cut(s) 619, 1058
BseSI GKGCMC 1 cut(s) 1258
BseXI GCAGC 2 cut(s) 28, 512
BseYI CCCAGC 2 cut(s) 155, 1268
Bsh1236I CGCG 1 cut(s) 858
BslFI GGGAC 4 cut(s) 205, 577, 796, 1247
BslI CCNNNNNNNGG 3 cut(s) 156, 177, 555
BsmAI GTCTC 1 cut(s) 112
BsmFI GGGAC 4 cut(s) 205, 577, 796, 1247
BsmI GAATGC 2 cut(s) 789, 1151
Bso31I GGTCTC 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 1258
Bsp143I GATC 1 cut(s) 690
BspACI CCGC 2 cut(s) 251, 996
BspCNI CTCAG 3 cut(s) 158, 185, 1156
BspFNI CGCG 1 cut(s) 858
BspLI GGNNCC 3 cut(s) 565, 889, 977
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 1 cut(s) 685
BspTNI GGTCTC 1 cut(s) 112
BsrDI GCAATG 3 cut(s) 346, 767, 1263
BsrI ACTGG 2 cut(s) 452, 695
BssECI CCNNGG 2 cut(s) 290, 681
BssMI GATC 1 cut(s) 690
BssSI CACGAG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 2 cut(s) 291, 682
Bst4CI ACNGT 4 cut(s) 269, 625, 646, 980
BstBAI YACGTR 1 cut(s) 1003
BstDEI CTNAG 4 cut(s) 145, 172, 389, 1164
BstF5I GGATG 1 cut(s) 545
BstFNI CGCG 1 cut(s) 858
BstHHI GCGC 2 cut(s) 1050, 1163
BstKTI GATC 1 cut(s) 693
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 1 cut(s) 690
BstMWI GCNNNNNNNGC 3 cut(s) 656, 686, 992
BstNI CCWGG 2 cut(s) 291, 682
BstNSI RCATGY 4 cut(s) 465, 638, 654, 1202
BstSCI CCNGG 2 cut(s) 289, 680
BstSFI CTRYAG 2 cut(s) 195, 550
BstSLI GKGCMC 1 cut(s) 1258
BstSNI TACGTA 1 cut(s) 1003
BstUI CGCG 1 cut(s) 858
BstV1I GCAGC 2 cut(s) 28, 512
BstV2I GAAGAC 2 cut(s) 281, 525
BstXI CCANNNNNNTGG 1 cut(s) 898
BtsCI GGATG 1 cut(s) 545
BtsI GCAGTG 2 cut(s) 150, 645
BtsIMutI CAGTG 4 cut(s) 150, 180, 587, 645
CaiI CAGNNNCTG 1 cut(s) 152
CfoI GCGC 2 cut(s) 1050, 1163
Cfr13I GGNCC 2 cut(s) 442, 564
Csp6I GTAC 4 cut(s) 312, 497, 848, 1121
CviAII CATG 7 cut(s) 462, 577, 635, 651, 947, 1068, 1199
CviJI RGCY 7 cut(s) 19, 149, 181, 326, 680, 773, 1133
CviKI_1 RGCY 7 cut(s) 19, 149, 181, 326, 680, 773, 1133
CviQI GTAC 4 cut(s) 312, 497, 848, 1121
DdeI CTNAG 4 cut(s) 145, 172, 389, 1164
DpnI GATC 1 cut(s) 692
DpnII GATC 1 cut(s) 690
Eco105I TACGTA 1 cut(s) 1003
Eco31I GGTCTC 1 cut(s) 112
Eco47I GGWCC 2 cut(s) 442, 564
EcoO109I RGGNCCY 2 cut(s) 442, 564
EcoRI GAATTC 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 289, 680
EcoT22I ATGCAT 1 cut(s) 1265
FaeI CATG 7 cut(s) 465, 580, 638, 654, 950, 1071, 1202
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 4 cut(s) 205, 577, 796, 1247
FatI CATG 7 cut(s) 461, 576, 634, 650, 946, 1067, 1198
Fnu4HI GCNGC 3 cut(s) 17, 526, 996
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 3 cut(s) 17, 526, 996
FspBI CTAG 3 cut(s) 446, 1053, 1124
GlaI GCGC 2 cut(s) 1049, 1162
GluI GCNGC 3 cut(s) 17, 526, 996
GsaI CCCAGC 2 cut(s) 159, 1272
HhaI GCGC 2 cut(s) 1050, 1163
Hin1II CATG 7 cut(s) 465, 580, 638, 654, 950, 1071, 1202
Hin6I GCGC 2 cut(s) 1048, 1161
HinP1I GCGC 2 cut(s) 1048, 1161
HinfI GANTC 2 cut(s) 22, 797
HphI GGTGA 2 cut(s) 980, 1151
Hpy166II GTNNAC 1 cut(s) 230
Hpy188I TCNGA 2 cut(s) 676, 802
Hpy188III TCNNGA 7 cut(s) 262, 408, 470, 667, 794, 1172, 1228
Hpy8I GTNNAC 1 cut(s) 230
HpyAV CCTTC 2 cut(s) 526, 861
HpyCH4III ACNGT 4 cut(s) 269, 625, 646, 980
HpyCH4IV ACGT 1 cut(s) 1002
HpyF10VI GCNNNNNNNGC 3 cut(s) 656, 686, 992
HpyF3I CTNAG 4 cut(s) 145, 172, 389, 1164
HpySE526I ACGT 1 cut(s) 1002
Hsp92II CATG 7 cut(s) 465, 580, 638, 654, 950, 1071, 1202
HspAI GCGC 2 cut(s) 1048, 1161
Kzo9I GATC 1 cut(s) 690
LmnI GCTCC 1 cut(s) 1045
Lsp1109I GCAGC 2 cut(s) 28, 512
LweI GCATC 1 cut(s) 267
MaeI CTAG 3 cut(s) 446, 1053, 1124
MaeII ACGT 1 cut(s) 1002
MaeIII GTNAC 2 cut(s) 5, 640
MalI GATC 1 cut(s) 692
MboI GATC 1 cut(s) 690
MboII GAAGA 5 cut(s) 286, 503, 530, 935, 1154
MhlI GDGCHC 1 cut(s) 1258
MluCI AATT 8 cut(s) 299, 373, 411, 670, 735, 933, 951, 990
MmeI TCCRAC 2 cut(s) 607, 901
MnlI CCTC 8 cut(s) 181, 333, 554, 597, 600, 1002, 1036, 1224
Mph1103I ATGCAT 1 cut(s) 1265
MseI TTAA 3 cut(s) 36, 59, 738
MslI CAYNNNNRTG 4 cut(s) 363, 616, 896, 914
MspR9I CCNGG 2 cut(s) 291, 682
Mva1269I GAATGC 2 cut(s) 789, 1151
MvaI CCWGG 2 cut(s) 291, 682
MvnI CGCG 1 cut(s) 858
MwoI GCNNNNNNNGC 3 cut(s) 656, 686, 992
NdeII GATC 1 cut(s) 690
NlaIII CATG 7 cut(s) 465, 580, 638, 654, 950, 1071, 1202
NlaIV GGNNCC 3 cut(s) 565, 889, 977
NmuCI GTSAC 2 cut(s) 5, 640
NsiI ATGCAT 1 cut(s) 1265
NspI RCATGY 4 cut(s) 465, 638, 654, 1202
PciI ACATGT 1 cut(s) 461
PctI GAATGC 2 cut(s) 789, 1151
PfeI GAWTC 2 cut(s) 22, 797
PkrI GCNGC 3 cut(s) 18, 527, 997
Ppu21I YACGTR 1 cut(s) 1003
PpuMI RGGWCCY 2 cut(s) 442, 564
PscI ACATGT 1 cut(s) 461
Psp5II RGGWCCY 2 cut(s) 442, 564
Psp6I CCWGG 2 cut(s) 289, 680
PspFI CCCAGC 2 cut(s) 155, 1268
PspGI CCWGG 2 cut(s) 289, 680
PspN4I GGNNCC 3 cut(s) 565, 889, 977
PspPI GGNCC 2 cut(s) 442, 564
PspPPI RGGWCCY 2 cut(s) 442, 564
PstI CTGCAG 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 152
RsaI GTAC 4 cut(s) 313, 498, 849, 1122
RsaNI GTAC 4 cut(s) 312, 497, 848, 1121
RseI CAYNNNNRTG 4 cut(s) 363, 616, 896, 914
SaqAI TTAA 3 cut(s) 36, 59, 738
SatI GCNGC 3 cut(s) 17, 526, 996
Sau3AI GATC 1 cut(s) 690
Sau96I GGNCC 2 cut(s) 442, 564
ScaI AGTACT 1 cut(s) 1122
ScrFI CCNGG 2 cut(s) 291, 682
SduI GDGCHC 1 cut(s) 1258
SfaNI GCATC 1 cut(s) 267
SfcI CTRYAG 2 cut(s) 195, 550
SinI GGWCC 2 cut(s) 442, 564
SmiMI CAYNNNNRTG 4 cut(s) 363, 616, 896, 914
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SnaBI TACGTA 1 cut(s) 1003
SpeI ACTAGT 1 cut(s) 1123
Sse9I AATT 8 cut(s) 299, 373, 411, 670, 735, 933, 951, 990
SsiI CCGC 2 cut(s) 251, 996
SspMI CTAG 3 cut(s) 446, 1053, 1124
StyD4I CCNGG 2 cut(s) 289, 680
TaaI ACNGT 4 cut(s) 269, 625, 646, 980
TaiI ACGT 1 cut(s) 1005
TaqI TCGA 2 cut(s) 321, 530
TasI AATT 8 cut(s) 299, 373, 411, 670, 735, 933, 951, 990
TatI WGTACW 2 cut(s) 496, 1120
TauI GCSGC 1 cut(s) 998
TfiI GAWTC 2 cut(s) 22, 797
Tru1I TTAA 3 cut(s) 36, 59, 738
Tru9I TTAA 3 cut(s) 36, 59, 738
TscAI CASTG 4 cut(s) 157, 180, 594, 645
TseFI GTSAC 2 cut(s) 5, 640
TseI GCWGC 2 cut(s) 16, 525
Tsp45I GTSAC 2 cut(s) 5, 640
TspDTI ATGAA 4 cut(s) 534, 565, 609, 935
TspRI CASTG 4 cut(s) 157, 180, 594, 645
VpaK11BI GGWCC 2 cut(s) 442, 564
XapI RAATTY 4 cut(s) 299, 373, 411, 670
XceI RCATGY 4 cut(s) 465, 638, 654, 1202
XspI CTAG 3 cut(s) 446, 1053, 1124
ZrmI AGTACT 1 cut(s) 1122
Zsp2I ATGCAT 1 cut(s) 1265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.