Threonine dehydratase biosynthetic
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 513 |
| Acc65I | GGTACC | 1 cut(s) | 155 |
| AccB1I | GGYRCC | 1 cut(s) | 155 |
| AccII | CGCG | 1 cut(s) | 1160 |
| AciI | CCGC | 2 cut(s) | 296, 1158 |
| AclI | AACGTT | 1 cut(s) | 34 |
| AclWI | GGATC | 1 cut(s) | 360 |
| AcsI | RAATTY | 2 cut(s) | 909, 1191 |
| AcuI | CTGAAG | 2 cut(s) | 984, 1008 |
| AfaI | GTAC | 9 cut(s) | 92, 157, 398, 410, 475, 619, 677, 1163, 1188 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 438, 794 |
| AgsI | TTSAA | 9 cut(s) | 39, 235, 611, 699, 753, 794, 904, 915, 1463 |
| AjnI | CCWGG | 1 cut(s) | 729 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1278, 1310 |
| AluBI | AGCT | 4 cut(s) | 122, 208, 494, 1475 |
| AluI | AGCT | 4 cut(s) | 122, 208, 494, 1475 |
| Alw21I | GWGCWC | 2 cut(s) | 514, 1384 |
| Alw26I | GTCTC | 1 cut(s) | 408 |
| Alw44I | GTGCAC | 1 cut(s) | 510 |
| AlwI | GGATC | 1 cut(s) | 360 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 665 |
| Ama87I | CYCGRG | 2 cut(s) | 209, 1425 |
| ApaLI | GTGCAC | 1 cut(s) | 510 |
| ApeKI | GCWGC | 3 cut(s) | 494, 601, 659 |
| ApoI | RAATTY | 2 cut(s) | 909, 1191 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1080 |
| Asp718I | GGTACC | 1 cut(s) | 155 |
| AspLEI | GCGC | 1 cut(s) | 255 |
| AspS9I | GGNCC | 1 cut(s) | 727 |
| AsuHPI | GGTGA | 5 cut(s) | 179, 623, 1142, 1310, 1490 |
| AvaI | CYCGRG | 2 cut(s) | 209, 1425 |
| AvaII | GGWCC | 1 cut(s) | 727 |
| BaeGI | GKGCMC | 1 cut(s) | 514 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 102, 135, 609, 642 |
| BanI | GGYRCC | 1 cut(s) | 155 |
| BbsI | GAAGAC | 2 cut(s) | 563, 972 |
| Bbv12I | GWGCWC | 2 cut(s) | 514, 1384 |
| BbvI | GCAGC | 3 cut(s) | 481, 613, 671 |
| BccI | CCATC | 4 cut(s) | 36, 83, 167, 1127 |
| BceAI | ACGGC | 1 cut(s) | 1322 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1091, 1125 |
| BciT130I | CCWGG | 1 cut(s) | 731 |
| BclI | TGATCA | 1 cut(s) | 1501 |
| BcoDI | GTCTC | 1 cut(s) | 408 |
| BfaI | CTAG | 2 cut(s) | 335, 758 |
| BfuAI | ACCTGC | 1 cut(s) | 513 |
| BisI | GCNGC | 4 cut(s) | 495, 602, 660, 1158 |
| BlsI | GCNGC | 4 cut(s) | 496, 603, 661, 1159 |
| Bme1390I | CCNGG | 1 cut(s) | 731 |
| Bme18I | GGWCC | 1 cut(s) | 727 |
| BmeT110I | CYCGRG | 2 cut(s) | 209, 1425 |
| BmgT120I | GGNCC | 1 cut(s) | 727 |
| BmiI | GGNNCC | 3 cut(s) | 157, 728, 735 |
| BmrFI | CCNGG | 1 cut(s) | 731 |
| BmsI | GCATC | 7 cut(s) | 318, 328, 506, 646, 733, 960, 1324 |
| BpiI | GAAGAC | 2 cut(s) | 563, 972 |
| BpuEI | CTTGAG | 1 cut(s) | 656 |
| BsaAI | YACGTR | 2 cut(s) | 1009, 1165 |
| BsaJI | CCNNGG | 1 cut(s) | 837 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 438, 794 |
| Bse1I | ACTGG | 2 cut(s) | 1124, 1369 |
| Bse3DI | GCAATG | 2 cut(s) | 573, 774 |
| BseBI | CCWGG | 1 cut(s) | 731 |
| BseDI | CCNNGG | 1 cut(s) | 837 |
| BseGI | GGATG | 2 cut(s) | 922, 951 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 438, 794 |
| BseMI | GCAATG | 2 cut(s) | 573, 774 |
| BseMII | CTCAG | 1 cut(s) | 593 |
| BseNI | ACTGG | 2 cut(s) | 1124, 1369 |
| BseSI | GKGCMC | 1 cut(s) | 514 |
| BseXI | GCAGC | 3 cut(s) | 481, 613, 671 |
| BseYI | CCCAGC | 1 cut(s) | 1394 |
| Bsh1236I | CGCG | 1 cut(s) | 1160 |
| BshNI | GGYRCC | 1 cut(s) | 155 |
| BsiHKAI | GWGCWC | 2 cut(s) | 514, 1384 |
| BsiHKCI | CYCGRG | 2 cut(s) | 209, 1425 |
| BsiWI | CGTACG | 1 cut(s) | 1161 |
| BslFI | GGGAC | 3 cut(s) | 740, 775, 1063 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 438, 794 |
| BsmAI | GTCTC | 1 cut(s) | 408 |
| BsmFI | GGGAC | 3 cut(s) | 740, 775, 1063 |
| BsmI | GAATGC | 1 cut(s) | 1310 |
| BsoBI | CYCGRG | 2 cut(s) | 209, 1425 |
| Bsp1286I | GDGCHC | 2 cut(s) | 514, 1384 |
| Bsp1407I | TGTACA | 1 cut(s) | 396 |
| Bsp143I | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| BspACI | CCGC | 2 cut(s) | 296, 1158 |
| BspCNI | CTCAG | 1 cut(s) | 592 |
| BspFNI | CGCG | 1 cut(s) | 1160 |
| BspHI | TCATGA | 1 cut(s) | 132 |
| BspLI | GGNNCC | 3 cut(s) | 157, 728, 735 |
| BspMI | ACCTGC | 1 cut(s) | 513 |
| BspPI | GGATC | 1 cut(s) | 360 |
| BspT107I | GGYRCC | 1 cut(s) | 155 |
| BsrDI | GCAATG | 2 cut(s) | 573, 774 |
| BsrGI | TGTACA | 1 cut(s) | 396 |
| BsrI | ACTGG | 2 cut(s) | 1124, 1369 |
| BssECI | CCNNGG | 1 cut(s) | 837 |
| BssMI | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| BssT1I | CCWWGG | 1 cut(s) | 837 |
| Bst2UI | CCWGG | 1 cut(s) | 731 |
| Bst4CI | ACNGT | 3 cut(s) | 95, 478, 1211 |
| Bst6I | CTCTTC | 3 cut(s) | 700, 1458, 1476 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 665 |
| BstAUI | TGTACA | 1 cut(s) | 396 |
| BstBAI | YACGTR | 2 cut(s) | 1009, 1165 |
| BstC8I | GCNNGC | 4 cut(s) | 492, 510, 834, 897 |
| BstDEI | CTNAG | 2 cut(s) | 123, 579 |
| BstEII | GGTNACC | 1 cut(s) | 1136 |
| BstF5I | GGATG | 2 cut(s) | 922, 951 |
| BstFNI | CGCG | 1 cut(s) | 1160 |
| BstHHI | GCGC | 1 cut(s) | 255 |
| BstKTI | GATC | 5 cut(s) | 355, 994, 1068, 1105, 1504 |
| BstMAI | GTCTC | 1 cut(s) | 408 |
| BstMBI | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 665, 1154 |
| BstNI | CCWGG | 1 cut(s) | 731 |
| BstNSI | RCATGY | 3 cut(s) | 750, 819, 899 |
| BstPI | GGTNACC | 1 cut(s) | 1136 |
| BstSCI | CCNGG | 1 cut(s) | 729 |
| BstSLI | GKGCMC | 1 cut(s) | 514 |
| BstSNI | TACGTA | 1 cut(s) | 1165 |
| BstUI | CGCG | 1 cut(s) | 1160 |
| BstV1I | GCAGC | 3 cut(s) | 481, 613, 671 |
| BstV2I | GAAGAC | 2 cut(s) | 563, 972 |
| BtsCI | GGATG | 2 cut(s) | 922, 951 |
| BtsI | GCAGTG | 1 cut(s) | 663 |
| BtsIMutI | CAGTG | 3 cut(s) | 663, 1026, 1362 |
| BveI | ACCTGC | 1 cut(s) | 513 |
| Cac8I | GCNNGC | 4 cut(s) | 492, 510, 834, 897 |
| CaiI | CAGNNNCTG | 1 cut(s) | 665 |
| CciI | TCATGA | 1 cut(s) | 132 |
| CfoI | GCGC | 1 cut(s) | 255 |
| Cfr13I | GGNCC | 1 cut(s) | 727 |
| Csp6I | GTAC | 9 cut(s) | 91, 156, 397, 409, 474, 618, 676, 1162, 1187 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 330, 365 |
| CviQI | GTAC | 9 cut(s) | 91, 156, 397, 409, 474, 618, 676, 1162, 1187 |
| DdeI | CTNAG | 2 cut(s) | 123, 579 |
| DpnI | GATC | 5 cut(s) | 354, 993, 1067, 1104, 1503 |
| DpnII | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| Eam1104I | CTCTTC | 3 cut(s) | 700, 1458, 1476 |
| EarI | CTCTTC | 3 cut(s) | 700, 1458, 1476 |
| Eco105I | TACGTA | 1 cut(s) | 1165 |
| Eco130I | CCWWGG | 1 cut(s) | 837 |
| Eco47I | GGWCC | 1 cut(s) | 727 |
| Eco57I | CTGAAG | 2 cut(s) | 984, 1008 |
| Eco88I | CYCGRG | 2 cut(s) | 209, 1425 |
| Eco91I | GGTNACC | 1 cut(s) | 1136 |
| EcoO109I | RGGNCCY | 1 cut(s) | 727 |
| EcoO65I | GGTNACC | 1 cut(s) | 1136 |
| EcoRII | CCWGG | 1 cut(s) | 729 |
| EcoT14I | CCWWGG | 1 cut(s) | 837 |
| EcoT22I | ATGCAT | 3 cut(s) | 311, 748, 901 |
| ErhI | CCWWGG | 1 cut(s) | 837 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 577, 609 |
| FaqI | GGGAC | 3 cut(s) | 740, 775, 1063 |
| FauNDI | CATATG | 1 cut(s) | 1316 |
| FbaI | TGATCA | 1 cut(s) | 1501 |
| Fnu4HI | GCNGC | 4 cut(s) | 495, 602, 660, 1158 |
| FokI | GGATG | 2 cut(s) | 909, 938 |
| Fsp4HI | GCNGC | 4 cut(s) | 495, 602, 660, 1158 |
| FspBI | CTAG | 2 cut(s) | 335, 758 |
| GlaI | GCGC | 1 cut(s) | 254 |
| GluI | GCNGC | 4 cut(s) | 495, 602, 660, 1158 |
| GsaI | CCCAGC | 1 cut(s) | 1398 |
| HhaI | GCGC | 1 cut(s) | 255 |
| Hin6I | GCGC | 1 cut(s) | 253 |
| HinP1I | GCGC | 1 cut(s) | 253 |
| HincII | GTYRAC | 1 cut(s) | 367 |
| HindII | GTYRAC | 1 cut(s) | 367 |
| HinfI | GANTC | 5 cut(s) | 50, 136, 315, 904, 959 |
| HpaI | GTTAAC | 1 cut(s) | 367 |
| HphI | GGTGA | 5 cut(s) | 179, 623, 1142, 1310, 1490 |
| Hpy166II | GTNNAC | 4 cut(s) | 170, 367, 399, 512 |
| Hpy188I | TCNGA | 6 cut(s) | 314, 827, 889, 956, 964, 1324 |
| Hpy8I | GTNNAC | 4 cut(s) | 170, 367, 399, 512 |
| HpyAV | CCTTC | 3 cut(s) | 541, 788, 796 |
| HpyCH4III | ACNGT | 3 cut(s) | 95, 478, 1211 |
| HpyCH4IV | ACGT | 4 cut(s) | 17, 34, 1008, 1164 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 665, 1154 |
| HpyF3I | CTNAG | 2 cut(s) | 123, 579 |
| HpySE526I | ACGT | 4 cut(s) | 17, 34, 1008, 1164 |
| HspAI | GCGC | 1 cut(s) | 253 |
| KpnI | GGTACC | 1 cut(s) | 159 |
| Ksp22I | TGATCA | 1 cut(s) | 1501 |
| KspAI | GTTAAC | 1 cut(s) | 367 |
| Kzo9I | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| Lsp1109I | GCAGC | 3 cut(s) | 481, 613, 671 |
| LweI | GCATC | 7 cut(s) | 318, 328, 506, 646, 733, 960, 1324 |
| MaeI | CTAG | 2 cut(s) | 335, 758 |
| MaeII | ACGT | 4 cut(s) | 17, 34, 1008, 1164 |
| MaeIII | GTNAC | 6 cut(s) | 5, 13, 70, 625, 1019, 1136 |
| MalI | GATC | 5 cut(s) | 354, 993, 1067, 1104, 1503 |
| MboI | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| MfeI | CAATTG | 3 cut(s) | 84, 789, 1053 |
| MhlI | GDGCHC | 2 cut(s) | 514, 1384 |
| MlyI | GAGTC | 2 cut(s) | 44, 968 |
| MmeI | TCCRAC | 2 cut(s) | 503, 1242 |
| Mph1103I | ATGCAT | 3 cut(s) | 311, 748, 901 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1080 |
| MseI | TTAA | 2 cut(s) | 366, 1242 |
| MslI | CAYNNNNRTG | 1 cut(s) | 927 |
| MspR9I | CCNGG | 1 cut(s) | 731 |
| MunI | CAATTG | 3 cut(s) | 84, 789, 1053 |
| Mva1269I | GAATGC | 1 cut(s) | 1310 |
| MvaI | CCWGG | 1 cut(s) | 731 |
| MvnI | CGCG | 1 cut(s) | 1160 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 665, 1154 |
| NdeI | CATATG | 1 cut(s) | 1316 |
| NdeII | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| NlaIV | GGNNCC | 3 cut(s) | 157, 728, 735 |
| NmuCI | GTSAC | 1 cut(s) | 1019 |
| NsiI | ATGCAT | 3 cut(s) | 311, 748, 901 |
| NspI | RCATGY | 3 cut(s) | 750, 819, 899 |
| PaeI | GCATGC | 1 cut(s) | 899 |
| PaeR7I | CTCGAG | 2 cut(s) | 209, 1425 |
| PagI | TCATGA | 1 cut(s) | 132 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1432 |
| PctI | GAATGC | 1 cut(s) | 1310 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1080 |
| PfeI | GAWTC | 3 cut(s) | 136, 315, 904 |
| Pfl23II | CGTACG | 1 cut(s) | 1161 |
| PkrI | GCNGC | 4 cut(s) | 496, 603, 661, 1159 |
| PleI | GAGTC | 2 cut(s) | 44, 967 |
| PpsI | GAGTC | 2 cut(s) | 44, 967 |
| Ppu21I | YACGTR | 2 cut(s) | 1009, 1165 |
| PpuMI | RGGWCCY | 1 cut(s) | 727 |
| Psp1406I | AACGTT | 1 cut(s) | 34 |
| Psp5II | RGGWCCY | 1 cut(s) | 727 |
| Psp6I | CCWGG | 1 cut(s) | 729 |
| PspEI | GGTNACC | 1 cut(s) | 1136 |
| PspFI | CCCAGC | 1 cut(s) | 1394 |
| PspGI | CCWGG | 1 cut(s) | 729 |
| PspLI | CGTACG | 1 cut(s) | 1161 |
| PspN4I | GGNNCC | 3 cut(s) | 157, 728, 735 |
| PspPI | GGNCC | 1 cut(s) | 727 |
| PspPPI | RGGWCCY | 1 cut(s) | 727 |
| PstNI | CAGNNNCTG | 1 cut(s) | 665 |
| RsaI | GTAC | 9 cut(s) | 92, 157, 398, 410, 475, 619, 677, 1163, 1188 |
| RsaNI | GTAC | 9 cut(s) | 91, 156, 397, 409, 474, 618, 676, 1162, 1187 |
| RseI | CAYNNNNRTG | 1 cut(s) | 927 |
| SaqAI | TTAA | 2 cut(s) | 366, 1242 |
| SatI | GCNGC | 4 cut(s) | 495, 602, 660, 1158 |
| Sau3AI | GATC | 5 cut(s) | 352, 991, 1065, 1102, 1501 |
| Sau96I | GGNCC | 1 cut(s) | 727 |
| SchI | GAGTC | 2 cut(s) | 44, 968 |
| ScrFI | CCNGG | 1 cut(s) | 731 |
| SduI | GDGCHC | 2 cut(s) | 514, 1384 |
| SfaNI | GCATC | 7 cut(s) | 318, 328, 506, 646, 733, 960, 1324 |
| Sfr274I | CTCGAG | 2 cut(s) | 209, 1425 |
| SinI | GGWCC | 1 cut(s) | 727 |
| SlaI | CTCGAG | 2 cut(s) | 209, 1425 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 927 |
| SmlI | CTYRAG | 3 cut(s) | 209, 671, 1425 |
| SmoI | CTYRAG | 3 cut(s) | 209, 671, 1425 |
| SnaBI | TACGTA | 1 cut(s) | 1165 |
| SphI | GCATGC | 1 cut(s) | 899 |
| SsiI | CCGC | 2 cut(s) | 296, 1158 |
| SspI | AATATT | 1 cut(s) | 1421 |
| SspMI | CTAG | 2 cut(s) | 335, 758 |
| StyD4I | CCNGG | 1 cut(s) | 729 |
| StyI | CCWWGG | 1 cut(s) | 837 |
| TaaI | ACNGT | 3 cut(s) | 95, 478, 1211 |
| TaiI | ACGT | 4 cut(s) | 20, 37, 1011, 1167 |
| TaqI | TCGA | 6 cut(s) | 48, 210, 355, 907, 1084, 1426 |
| TaqII | GACCGA | 1 cut(s) | 373 |
| TatI | WGTACW | 2 cut(s) | 396, 675 |
| TauI | GCSGC | 1 cut(s) | 1160 |
| TfiI | GAWTC | 3 cut(s) | 136, 315, 904 |
| Tru1I | TTAA | 2 cut(s) | 366, 1242 |
| Tru9I | TTAA | 2 cut(s) | 366, 1242 |
| TscAI | CASTG | 3 cut(s) | 670, 1033, 1369 |
| TseFI | GTSAC | 1 cut(s) | 1019 |
| TseI | GCWGC | 3 cut(s) | 494, 601, 659 |
| Tsp45I | GTSAC | 1 cut(s) | 1019 |
| TspDTI | ATGAA | 3 cut(s) | 763, 1089, 1506 |
| TspGWI | ACGGA | 2 cut(s) | 426, 698 |
| TspRI | CASTG | 3 cut(s) | 670, 1033, 1369 |
| VneI | GTGCAC | 1 cut(s) | 510 |
| VpaK11BI | GGWCC | 1 cut(s) | 727 |
| XapI | RAATTY | 2 cut(s) | 909, 1191 |
| XbaI | TCTAGA | 1 cut(s) | 334 |
| XceI | RCATGY | 3 cut(s) | 750, 819, 899 |
| XhoI | CTCGAG | 2 cut(s) | 209, 1425 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1080 |
| XspI | CTAG | 2 cut(s) | 335, 758 |
| Zsp2I | ATGCAT | 3 cut(s) | 311, 748, 901 |