No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 646 |
| AciI | CCGC | 2 cut(s) | 30, 840 |
| AclWI | GGATC | 3 cut(s) | 53, 356, 574 |
| AcsI | RAATTY | 1 cut(s) | 848 |
| AcuI | CTGAAG | 2 cut(s) | 113, 685 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 371 |
| AgsI | TTSAA | 3 cut(s) | 37, 103, 929 |
| AluBI | AGCT | 4 cut(s) | 218, 299, 419, 908 |
| AluI | AGCT | 4 cut(s) | 218, 299, 419, 908 |
| Alw21I | GWGCWC | 2 cut(s) | 256, 515 |
| Alw26I | GTCTC | 1 cut(s) | 619 |
| AlwI | GGATC | 3 cut(s) | 53, 356, 574 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 950 |
| AoxI | GGCC | 2 cut(s) | 334, 484 |
| ApeKI | GCWGC | 3 cut(s) | 416, 419, 642 |
| ApoI | RAATTY | 1 cut(s) | 848 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 288, 320 |
| AspLEI | GCGC | 1 cut(s) | 647 |
| AspS9I | GGNCC | 3 cut(s) | 485, 584, 744 |
| AsuC2I | CCSGG | 1 cut(s) | 366 |
| AsuHPI | GGTGA | 1 cut(s) | 800 |
| AvaII | GGWCC | 2 cut(s) | 584, 744 |
| BaeGI | GKGCMC | 1 cut(s) | 275 |
| Bbv12I | GWGCWC | 2 cut(s) | 256, 515 |
| BbvI | GCAGC | 3 cut(s) | 406, 428, 629 |
| BccI | CCATC | 2 cut(s) | 36, 72 |
| BcnI | CCSGG | 1 cut(s) | 366 |
| BcoDI | GTCTC | 1 cut(s) | 619 |
| BfaI | CTAG | 1 cut(s) | 246 |
| BglII | AGATCT | 1 cut(s) | 863 |
| BisI | GCNGC | 5 cut(s) | 31, 417, 420, 643, 841 |
| BlsI | GCNGC | 5 cut(s) | 32, 418, 421, 644, 842 |
| Bme1390I | CCNGG | 1 cut(s) | 366 |
| Bme18I | GGWCC | 2 cut(s) | 584, 744 |
| BmgT120I | GGNCC | 3 cut(s) | 485, 584, 744 |
| BmrFI | CCNGG | 1 cut(s) | 366 |
| BmsI | GCATC | 2 cut(s) | 537, 956 |
| BpmI | CTGGAG | 1 cut(s) | 10 |
| BpuEI | CTTGAG | 1 cut(s) | 677 |
| BpuMI | CCSGG | 1 cut(s) | 366 |
| BsaAI | YACGTR | 1 cut(s) | 329 |
| BsaBI | GATNNNNATC | 1 cut(s) | 204 |
| BsaWI | WCCGGW | 1 cut(s) | 586 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 371 |
| Bse1I | ACTGG | 1 cut(s) | 458 |
| Bse3DI | GCAATG | 2 cut(s) | 624, 829 |
| Bse8I | GATNNNNATC | 1 cut(s) | 204 |
| BseGI | GGATG | 3 cut(s) | 64, 118, 940 |
| BseJI | GATNNNNATC | 1 cut(s) | 204 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 371 |
| BseMI | GCAATG | 2 cut(s) | 624, 829 |
| BseMII | CTCAG | 2 cut(s) | 485, 614 |
| BseNI | ACTGG | 1 cut(s) | 458 |
| BseRI | GAGGAG | 1 cut(s) | 264 |
| BseSI | GKGCMC | 1 cut(s) | 275 |
| BseXI | GCAGC | 3 cut(s) | 406, 428, 629 |
| BshFI | GGCC | 2 cut(s) | 336, 486 |
| BsiHKAI | GWGCWC | 2 cut(s) | 256, 515 |
| BsiSI | CCGG | 2 cut(s) | 365, 587 |
| BslFI | GGGAC | 1 cut(s) | 573 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 371 |
| BsmAI | GTCTC | 1 cut(s) | 619 |
| BsmFI | GGGAC | 1 cut(s) | 573 |
| BsnI | GGCC | 2 cut(s) | 336, 486 |
| Bsp1286I | GDGCHC | 3 cut(s) | 256, 275, 515 |
| Bsp143I | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| BspACI | CCGC | 2 cut(s) | 30, 840 |
| BspANI | GGCC | 2 cut(s) | 336, 486 |
| BspCNI | CTCAG | 2 cut(s) | 484, 615 |
| BspHI | TCATGA | 2 cut(s) | 196, 802 |
| BspPI | GGATC | 3 cut(s) | 53, 356, 574 |
| BsrDI | GCAATG | 2 cut(s) | 624, 829 |
| BsrI | ACTGG | 1 cut(s) | 458 |
| BssMI | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| Bst4CI | ACNGT | 2 cut(s) | 446, 815 |
| Bst6I | CTCTTC | 2 cut(s) | 261, 924 |
| BstBAI | YACGTR | 1 cut(s) | 329 |
| BstC8I | GCNNGC | 1 cut(s) | 659 |
| BstDEI | CTNAG | 2 cut(s) | 471, 623 |
| BstF5I | GGATG | 3 cut(s) | 64, 118, 940 |
| BstHHI | GCGC | 1 cut(s) | 647 |
| BstKTI | GATC | 5 cut(s) | 48, 364, 391, 569, 866 |
| BstMAI | GTCTC | 1 cut(s) | 619 |
| BstMBI | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 642, 840 |
| BstSCI | CCNGG | 1 cut(s) | 364 |
| BstSLI | GKGCMC | 1 cut(s) | 275 |
| BstSNI | TACGTA | 1 cut(s) | 329 |
| BstV1I | GCAGC | 3 cut(s) | 406, 428, 629 |
| BstX2I | RGATCY | 2 cut(s) | 566, 863 |
| BstYI | RGATCY | 2 cut(s) | 566, 863 |
| BsuRI | GGCC | 2 cut(s) | 336, 486 |
| BtsCI | GGATG | 3 cut(s) | 64, 118, 940 |
| Cac8I | GCNNGC | 1 cut(s) | 659 |
| CaiI | CAGNNNCTG | 1 cut(s) | 950 |
| CciI | TCATGA | 2 cut(s) | 196, 802 |
| CfoI | GCGC | 1 cut(s) | 647 |
| Cfr13I | GGNCC | 3 cut(s) | 485, 584, 744 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 745, 780 |
| CviAII | CATG | 7 cut(s) | 55, 197, 501, 563, 734, 803, 902 |
| DdeI | CTNAG | 2 cut(s) | 471, 623 |
| DpnI | GATC | 5 cut(s) | 47, 363, 390, 568, 865 |
| DpnII | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| Eam1104I | CTCTTC | 2 cut(s) | 261, 924 |
| EarI | CTCTTC | 2 cut(s) | 261, 924 |
| Eco105I | TACGTA | 1 cut(s) | 329 |
| Eco147I | AGGCCT | 1 cut(s) | 336 |
| Eco32I | GATATC | 1 cut(s) | 916 |
| Eco47I | GGWCC | 2 cut(s) | 584, 744 |
| Eco57I | CTGAAG | 2 cut(s) | 113, 685 |
| EcoO109I | RGGNCCY | 1 cut(s) | 744 |
| EcoRV | GATATC | 1 cut(s) | 916 |
| EcoT22I | ATGCAT | 1 cut(s) | 552 |
| FaeI | CATG | 7 cut(s) | 58, 200, 504, 566, 737, 806, 905 |
| FaqI | GGGAC | 1 cut(s) | 573 |
| FatI | CATG | 7 cut(s) | 54, 196, 500, 562, 733, 802, 901 |
| FauNDI | CATATG | 2 cut(s) | 552, 965 |
| Fnu4HI | GCNGC | 5 cut(s) | 31, 417, 420, 643, 841 |
| FokI | GGATG | 3 cut(s) | 51, 105, 947 |
| Fsp4HI | GCNGC | 5 cut(s) | 31, 417, 420, 643, 841 |
| FspBI | CTAG | 1 cut(s) | 246 |
| FspI | TGCGCA | 1 cut(s) | 646 |
| GlaI | GCGC | 1 cut(s) | 646 |
| GluI | GCNGC | 5 cut(s) | 31, 417, 420, 643, 841 |
| GsuI | CTGGAG | 1 cut(s) | 10 |
| HaeIII | GGCC | 2 cut(s) | 336, 486 |
| HapII | CCGG | 2 cut(s) | 365, 587 |
| HhaI | GCGC | 1 cut(s) | 647 |
| Hin1II | CATG | 7 cut(s) | 58, 200, 504, 566, 737, 806, 905 |
| Hin6I | GCGC | 1 cut(s) | 645 |
| HinP1I | GCGC | 1 cut(s) | 645 |
| HinfI | GANTC | 6 cut(s) | 86, 200, 457, 475, 590, 627 |
| HpaII | CCGG | 2 cut(s) | 365, 587 |
| HphI | GGTGA | 1 cut(s) | 800 |
| Hpy166II | GTNNAC | 1 cut(s) | 430 |
| Hpy188I | TCNGA | 8 cut(s) | 85, 177, 210, 474, 704, 783, 847, 952 |
| Hpy188III | TCNNGA | 2 cut(s) | 197, 803 |
| Hpy8I | GTNNAC | 1 cut(s) | 430 |
| HpyAV | CCTTC | 4 cut(s) | 172, 477, 757, 898 |
| HpyCH4III | ACNGT | 2 cut(s) | 446, 815 |
| HpyCH4IV | ACGT | 1 cut(s) | 328 |
| HpyCH4V | TGCA | 5 cut(s) | 544, 550, 657, 756, 834 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 642, 840 |
| HpyF3I | CTNAG | 2 cut(s) | 471, 623 |
| HpySE526I | ACGT | 1 cut(s) | 328 |
| Hsp92II | CATG | 7 cut(s) | 58, 200, 504, 566, 737, 806, 905 |
| HspAI | GCGC | 1 cut(s) | 645 |
| Kzo9I | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| LmnI | GCTCC | 4 cut(s) | 251, 413, 518, 905 |
| LpnPI | CCDG | 6 cut(s) | 40, 378, 439, 562, 600, 957 |
| Lsp1109I | GCAGC | 3 cut(s) | 406, 428, 629 |
| LweI | GCATC | 2 cut(s) | 537, 956 |
| MaeI | CTAG | 1 cut(s) | 246 |
| MaeII | ACGT | 1 cut(s) | 328 |
| MaeIII | GTNAC | 2 cut(s) | 278, 771 |
| MalI | GATC | 5 cut(s) | 47, 363, 390, 568, 865 |
| MboI | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| MboII | GAAGA | 4 cut(s) | 87, 119, 278, 941 |
| MflI | RGATCY | 2 cut(s) | 566, 863 |
| MhlI | GDGCHC | 3 cut(s) | 256, 275, 515 |
| MluCI | AATT | 7 cut(s) | 16, 103, 257, 349, 407, 494, 848 |
| MlyI | GAGTC | 2 cut(s) | 584, 621 |
| MnlI | CCTC | 8 cut(s) | 69, 196, 242, 256, 262, 347, 516, 925 |
| Mph1103I | ATGCAT | 1 cut(s) | 552 |
| MseI | TTAA | 5 cut(s) | 227, 437, 525, 852, 882 |
| MslI | CAYNNNNRTG | 1 cut(s) | 617 |
| MspA1I | CMGCKG | 2 cut(s) | 30, 419 |
| MspI | CCGG | 2 cut(s) | 365, 587 |
| MspR9I | CCNGG | 1 cut(s) | 366 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 642, 840 |
| NciI | CCSGG | 1 cut(s) | 366 |
| NdeI | CATATG | 2 cut(s) | 552, 965 |
| NdeII | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| NlaIII | CATG | 7 cut(s) | 58, 200, 504, 566, 737, 806, 905 |
| NmuCI | GTSAC | 2 cut(s) | 278, 771 |
| NsbI | TGCGCA | 1 cut(s) | 646 |
| NsiI | ATGCAT | 1 cut(s) | 552 |
| PagI | TCATGA | 2 cut(s) | 196, 802 |
| PceI | AGGCCT | 1 cut(s) | 336 |
| PfeI | GAWTC | 4 cut(s) | 86, 200, 457, 475 |
| PkrI | GCNGC | 5 cut(s) | 32, 418, 421, 644, 842 |
| PleI | GAGTC | 2 cut(s) | 584, 621 |
| PpsI | GAGTC | 2 cut(s) | 584, 621 |
| Ppu21I | YACGTR | 1 cut(s) | 329 |
| PpuMI | RGGWCCY | 1 cut(s) | 744 |
| Psp5II | RGGWCCY | 1 cut(s) | 744 |
| PspPI | GGNCC | 3 cut(s) | 485, 584, 744 |
| PspPPI | RGGWCCY | 1 cut(s) | 744 |
| PstNI | CAGNNNCTG | 1 cut(s) | 950 |
| PsuI | RGATCY | 2 cut(s) | 566, 863 |
| PvuII | CAGCTG | 1 cut(s) | 419 |
| RseI | CAYNNNNRTG | 1 cut(s) | 617 |
| SaqAI | TTAA | 5 cut(s) | 227, 437, 525, 852, 882 |
| SatI | GCNGC | 5 cut(s) | 31, 417, 420, 643, 841 |
| Sau3AI | GATC | 5 cut(s) | 45, 361, 388, 566, 863 |
| Sau96I | GGNCC | 3 cut(s) | 485, 584, 744 |
| SchI | GAGTC | 2 cut(s) | 584, 621 |
| ScrFI | CCNGG | 1 cut(s) | 366 |
| SduI | GDGCHC | 3 cut(s) | 256, 275, 515 |
| SetI | ASST | 9 cut(s) | 220, 301, 331, 421, 524, 655, 746, 790, 910 |
| SfaNI | GCATC | 2 cut(s) | 537, 956 |
| SinI | GGWCC | 2 cut(s) | 584, 744 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 617 |
| SmlI | CTYRAG | 1 cut(s) | 692 |
| SmoI | CTYRAG | 1 cut(s) | 692 |
| SnaBI | TACGTA | 1 cut(s) | 329 |
| Sse9I | AATT | 7 cut(s) | 16, 103, 257, 349, 407, 494, 848 |
| SseBI | AGGCCT | 1 cut(s) | 336 |
| SsiI | CCGC | 2 cut(s) | 30, 840 |
| SspMI | CTAG | 1 cut(s) | 246 |
| StuI | AGGCCT | 1 cut(s) | 336 |
| StyD4I | CCNGG | 1 cut(s) | 364 |
| TaaI | ACNGT | 2 cut(s) | 446, 815 |
| TaiI | ACGT | 1 cut(s) | 331 |
| TaqI | TCGA | 3 cut(s) | 306, 912, 918 |
| TasI | AATT | 7 cut(s) | 16, 103, 257, 349, 407, 494, 848 |
| TauI | GCSGC | 2 cut(s) | 33, 843 |
| TfiI | GAWTC | 4 cut(s) | 86, 200, 457, 475 |
| Tru1I | TTAA | 5 cut(s) | 227, 437, 525, 852, 882 |
| Tru9I | TTAA | 5 cut(s) | 227, 437, 525, 852, 882 |
| TseFI | GTSAC | 2 cut(s) | 278, 771 |
| TseI | GCWGC | 3 cut(s) | 416, 419, 642 |
| Tsp45I | GTSAC | 2 cut(s) | 278, 771 |
| TspDTI | ATGAA | 5 cut(s) | 43, 420, 739, 819, 954 |
| VpaK11BI | GGWCC | 2 cut(s) | 584, 744 |
| XapI | RAATTY | 1 cut(s) | 848 |
| XspI | CTAG | 1 cut(s) | 246 |
| Zsp2I | ATGCAT | 1 cut(s) | 552 |