Rh1CG020300

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000499
Physical Location & Seq
Reverse (-)
3381148 .. 3389909
8762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG020300.1

Sequence Viewer

Length: 708 bp
ATGGGAGGATCAACAGCAGCTGTTGGGAGGGGTGCAGCAAGTCTTTGTGTGCTTAATGACACACCTCCTCAGTCAGCACTTTCTTATAACATTTATGAAATTTCTGTTATTTTCACGACTATCTCAGGCTTTGGTACCGGCGAGAGAGTTGGTACATTTATTGACGCCTTTTATGTGGCTTACTGTACGGTCTTCTCAGTTGGTTTCTGCGATATTTCTCCTTTGAAGTCTCTTACTCGGATTCTTTGTGATGTGCTGGGATTGGTGGTTGGAACTATTGTAGTGATGGTACTCACTCATTTTTTGGGTCGGTTAGCATGCGAGTTGGGGCGGTGGATTTCATTTCGCTTTCTTATGAGATATCGAGTGTATTCAACTCTTGGTATGATCTTGCTGGCTCTATTTTTTGGGATGGCCGGCATTTATATCTTCGAGTGGCTGCACCTAGCTGGTGGATCTCCATCTCCATCTCCATCTCCTTCCACTATGTTACCTACTGATGCATACGCATGGGTCCTCTTTGTTGACATATTCCACCTCTCAGTTATGATGATGAATACCACTGGATTTGGAGATTTCGCCTTCACTACTGCATCTGGGAGAGCGTTTGCCATAAGCGGGGGCGCGGCGCTGGCGGTTGGGGGTGAGTCACAAAGCAGGCTATTCATGGAGCAAGCCATGAGCGAGCTCTTAACCTGGCGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

235

Amino Acids

25.42

Weight (kDa)

6.27

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 40 - 101 4.7e-06 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02510
fragaria_vesca FvH4_6g16970 FvH4_6g16980 FvH4_7g01220 FvH4_7g01220 FvH4_7g01220 FvH4_7g01240
malus_domestica MD05G1161500.v1.1
pyrus_communis pycom05g15160
rosa_chinensis RchiOBHm_Chr1g0316711 RchiOBHm_Chr1g0316801 RchiOBHm_Chr1g0316871 RchiOBHm_Chr1g0316881 RchiOBHm_Chr1g0316951 RchiOBHm_Chr3g0470991 RchiOBHm_Chr4g0402171
rosa_laevigata RLG00000001679 RLG00000001680 RLG00000007591 RLG00000009108 RLG00000024215 RLG00000024221 RLG00000030636 RLG00000030640 RLG00000030642 RLG00000030648 RLG00000030651 RLG00000030658 RLG00000030667
rosa_multiflora Rmu_sc0000839.1_g000010 Rmu_sc0002667.1_g000003 Rmu_sc0003008.1_g000023 Rmu_sc0003008.1_g000047 Rmu_sc0005604.1_g000001 Rmu_sc0005939.1_g000001 Rmu_sc0006790.1_g000011 Rmu_sc0007472.1_g000004 Rmu_sc0017515.1_g000001 Rmu_sc0019753.1_g000003 Rmu_sc0020172.1_g000001 Rmu_sc0023783.1_g000001
rosa_roxburghii Rroxscaffold_4G00330760 Rroxscaffold_4G00330800 Rroxscaffold_4G00330810 Rroxscaffold_4G00330950
rosa_rugosa Rorug01G0011200 Rorug01G0011500 Rorug01G0011600 Rorug01G0012300 Rorug01G0012300 Rorug01G0012400 Rorug01G0012500 Rorug01G0012600 Rorug01G0012600 Rorug01G0013400 Rorug01G0013400 Rorug01G0013500
rosa_samantha Rh1AG021100 Rh1AG022800 Rh1AG023300 Rh1AG023500 Rh1AG024000 Rh1AG024300 Rh1BG016000 Rh1BG016300 Rh1BG021300 Rh1BG021500 Rh1CG020300 Rh1CG022500 Rh1DG016300 Rh1DG016400 Rh1DG017300 Rh1DG017400 Rh1DG017700 Rh1DG017800 Rh1DG018200 Rh1DG019100 Rh1DG019900 Rh1DG020200 Rh1DG020800 Rh1DG021300 Rh1DG021400 Rh1DG021800 Rh3DG186400
rosa_wichuraiana Rw1G001370 Rw1G001480 Rw1G001650 Rw1G001680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 87
Acc65I GGTACC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 626
AciI CCGC 5 cut(s) 331, 618, 626, 635, 700
AclWI GGATC 2 cut(s) 16, 463
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 4 cut(s) 136, 154, 187, 291
AfiI CCNNNNNNNGG 2 cut(s) 618, 702
AgsI TTSAA 2 cut(s) 226, 375
AjnI CCWGG 1 cut(s) 695
AluBI AGCT 3 cut(s) 20, 449, 688
AluI AGCT 3 cut(s) 20, 449, 688
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 2 cut(s) 16, 463
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 3 cut(s) 17, 35, 439
ApoI RAATTY 1 cut(s) 99
Asp718I GGTACC 1 cut(s) 134
AspLEI GCGC 2 cut(s) 626, 631
AspS9I GGNCC 1 cut(s) 514
AsuHPI GGTGA 1 cut(s) 656
AvaII GGWCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 29, 47, 426
BccI CCATC 5 cut(s) 280, 406, 469, 475, 481
BciT130I CCWGG 1 cut(s) 697
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 1 cut(s) 446
BfoI RGCGCY 1 cut(s) 632
BisI GCNGC 4 cut(s) 18, 36, 440, 627
BlsI GCNGC 4 cut(s) 19, 37, 441, 628
Bme1390I CCNGG 1 cut(s) 697
Bme18I GGWCC 1 cut(s) 514
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 2 cut(s) 136, 515
BmrFI CCNGG 1 cut(s) 697
BmsI GCATC 2 cut(s) 490, 602
BpiI GAAGAC 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 618, 702
Bse118I RCCGGY 2 cut(s) 137, 416
Bse1I ACTGG 1 cut(s) 568
Bse8I GATNNNNATC 1 cut(s) 460
BseBI CCWGG 1 cut(s) 697
BseGI GGATG 1 cut(s) 417
BseJI GATNNNNATC 1 cut(s) 460
BseLI CCNNNNNNNGG 2 cut(s) 618, 702
BseMII CTCAG 4 cut(s) 83, 138, 210, 555
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 3 cut(s) 29, 47, 426
BseYI CCCAGC 1 cut(s) 256
BsgI GTGCAG 2 cut(s) 54, 425
Bsh1236I CGCG 1 cut(s) 626
BshFI GGCC 1 cut(s) 416
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 690
BsiSI CCGG 2 cut(s) 138, 417
BslI CCNNNNNNNGG 2 cut(s) 618, 702
BsmAI GTCTC 1 cut(s) 234
BsnI GGCC 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 690
Bsp143I GATC 3 cut(s) 8, 387, 455
BspACI CCGC 5 cut(s) 331, 618, 626, 635, 700
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 4 cut(s) 82, 137, 209, 554
BspFNI CGCG 1 cut(s) 626
BspLI GGNNCC 2 cut(s) 136, 515
BspPI GGATC 2 cut(s) 16, 463
BspT107I GGYRCC 1 cut(s) 134
BsrFI RCCGGY 2 cut(s) 137, 416
BsrI ACTGG 1 cut(s) 568
BssAI RCCGGY 2 cut(s) 137, 416
BssMI GATC 3 cut(s) 8, 387, 455
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 697
Bst4CI ACNGT 2 cut(s) 185, 190
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 7 cut(s) 319, 396, 418, 633, 659, 675, 686
BstDEI CTNAG 4 cut(s) 69, 124, 196, 541
BstENI CCTNNNNNAGG 1 cut(s) 700
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 1 cut(s) 626
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 2 cut(s) 626, 631
BstKTI GATC 3 cut(s) 11, 390, 458
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 3 cut(s) 8, 387, 455
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 1 cut(s) 697
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 695
BstUI CGCG 1 cut(s) 626
BstV1I GCAGC 3 cut(s) 29, 47, 426
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BsuRI GGCC 1 cut(s) 416
BtsCI GGATG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 7 cut(s) 319, 396, 418, 633, 659, 675, 686
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 2 cut(s) 626, 631
Cfr10I RCCGGY 2 cut(s) 137, 416
Cfr13I GGNCC 1 cut(s) 514
CseI GACGC 1 cut(s) 173
Csp6I GTAC 4 cut(s) 135, 153, 186, 290
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviAII CATG 4 cut(s) 318, 510, 667, 679
CviQI GTAC 4 cut(s) 135, 153, 186, 290
DdeI CTNAG 4 cut(s) 69, 124, 196, 541
DpnI GATC 3 cut(s) 10, 389, 457
DpnII GATC 3 cut(s) 8, 387, 455
EaeI YGGCCR 1 cut(s) 414
Ecl136II GAGCTC 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 690
Eco32I GATATC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 514
Eco53kI GAGCTC 1 cut(s) 688
EcoICRI GAGCTC 1 cut(s) 688
EcoNI CCTNNNNNAGG 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 695
EcoRV GATATC 1 cut(s) 362
EcoT22I ATGCAT 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 690
FaeI CATG 4 cut(s) 321, 513, 670, 682
FatI CATG 4 cut(s) 317, 509, 666, 678
FauI CCCGC 1 cut(s) 611
Fnu4HI GCNGC 4 cut(s) 18, 36, 440, 627
FokI GGATG 1 cut(s) 424
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 4 cut(s) 18, 36, 440, 627
FspBI CTAG 1 cut(s) 446
GlaI GCGC 2 cut(s) 625, 630
GluI GCNGC 4 cut(s) 18, 36, 440, 627
GsaI CCCAGC 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 1 cut(s) 416
HapII CCGG 2 cut(s) 138, 417
HgaI GACGC 1 cut(s) 173
HhaI GCGC 2 cut(s) 626, 631
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 4 cut(s) 321, 513, 670, 682
Hin6I GCGC 2 cut(s) 624, 629
HinP1I GCGC 2 cut(s) 624, 629
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 2 cut(s) 241, 647
HpaII CCGG 2 cut(s) 138, 417
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 1 cut(s) 240
Hpy188III TCNNGA 1 cut(s) 115
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 2 cut(s) 489, 592
HpyCH4III ACNGT 2 cut(s) 185, 190
HpyCH4V TGCA 4 cut(s) 35, 442, 503, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 4 cut(s) 69, 124, 196, 541
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 4 cut(s) 321, 513, 670, 682
HspAI GCGC 2 cut(s) 624, 629
KpnI GGTACC 1 cut(s) 138
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Kzo9I GATC 3 cut(s) 8, 387, 455
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 3 cut(s) 29, 47, 426
LweI GCATC 2 cut(s) 490, 602
MaeI CTAG 1 cut(s) 446
MaeIII GTNAC 2 cut(s) 489, 648
MalI GATC 3 cut(s) 10, 389, 457
MboI GATC 3 cut(s) 8, 387, 455
MboII GAAGA 2 cut(s) 184, 421
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 1 cut(s) 690
MluCI AATT 1 cut(s) 99
MlyI GAGTC 1 cut(s) 656
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 6 cut(s) 21, 75, 78, 527, 548, 696
Mph1103I ATGCAT 1 cut(s) 505
MroNI GCCGGC 1 cut(s) 416
MseI TTAA 2 cut(s) 54, 692
MslI CAYNNNNRTG 1 cut(s) 508
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 2 cut(s) 138, 417
MspR9I CCNGG 1 cut(s) 697
MteI GCGCNGCGC 1 cut(s) 627
MvaI CCWGG 1 cut(s) 697
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 1 cut(s) 632
NaeI GCCGGC 1 cut(s) 418
NdeII GATC 3 cut(s) 8, 387, 455
NgoMIV GCCGGC 1 cut(s) 416
NlaIII CATG 4 cut(s) 321, 513, 670, 682
NlaIV GGNNCC 2 cut(s) 136, 515
NmuCI GTSAC 1 cut(s) 648
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 1 cut(s) 321
PaeI GCATGC 1 cut(s) 321
PdiI GCCGGC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 4 cut(s) 19, 37, 441, 628
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PpuMI RGGWCCY 1 cut(s) 514
PsiI TTATAA 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 690
Psp5II RGGWCCY 1 cut(s) 514
Psp6I CCWGG 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 1 cut(s) 695
PspN4I GGNNCC 2 cut(s) 136, 515
PspPI GGNCC 1 cut(s) 514
PspPPI RGGWCCY 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 455
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 4 cut(s) 136, 154, 187, 291
RsaNI GTAC 4 cut(s) 135, 153, 186, 290
RseI CAYNNNNRTG 1 cut(s) 508
SacI GAGCTC 1 cut(s) 690
SaqAI TTAA 2 cut(s) 54, 692
SatI GCNGC 4 cut(s) 18, 36, 440, 627
Sau3AI GATC 3 cut(s) 8, 387, 455
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 1 cut(s) 656
ScrFI CCNGG 1 cut(s) 697
SduI GDGCHC 1 cut(s) 690
SetI ASST 9 cut(s) 22, 67, 447, 451, 496, 540, 690, 698, 707
SfaNI GCATC 2 cut(s) 490, 602
SinI GGWCC 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 508
SphI GCATGC 1 cut(s) 321
Sse9I AATT 1 cut(s) 99
SsiI CCGC 5 cut(s) 331, 618, 626, 635, 700
SspMI CTAG 1 cut(s) 446
SstI GAGCTC 1 cut(s) 690
StyD4I CCNGG 1 cut(s) 695
TaaI ACNGT 2 cut(s) 185, 190
TaqI TCGA 2 cut(s) 364, 432
TasI AATT 1 cut(s) 99
TauI GCSGC 1 cut(s) 629
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 2 cut(s) 54, 692
Tru9I TTAA 2 cut(s) 54, 692
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 1 cut(s) 648
TseI GCWGC 3 cut(s) 17, 35, 439
Tsp45I GTSAC 1 cut(s) 648
TspDTI ATGAA 4 cut(s) 111, 330, 569, 655
TspRI CASTG 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 514
XagI CCTNNNNNAGG 1 cut(s) 700
XapI RAATTY 1 cut(s) 99
XceI RCATGY 1 cut(s) 321
XspI CTAG 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.