RLG00000030667

Ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000499
Physical Location & Seq
Reverse (-)
65415160 .. 65417340
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030667

Sequence Viewer

Length: 1071 bp
ATGGAACGGACAAAAGAAAGAGTTGTAGCTGATTTGAGAAGACATTCGGTCCAAACATCTGAATTTGAAAAGGAACTCAACGATCTGAACAATGCCTTGAAACGTGAGGAGAAGTCTGAAACCAATCTAAAAGATATTTTTGACACGATGCAAGCTGAGCATGATGAATATAGGAAAGCTAAGGAAAAACAGCTGACAGAAGTAGAGTGTTCGTTGGATATTGTAGAGAAGAGATTGTCAGTGACGCTAGAAAATCGTGTAGAGATGGAGAAGAGACTAGAAAATCTTAATGTTTTCTGTGAGGCTGTGTATTCTACTCTCAAATCGAAGGAGTCTAGCCACGTCTCTCTTGTCAAAGAAGCTCAGCTTGATCATCCTGGTGGACAGAATGGACAGAAAACGCGAAAAGACGAGGAACTCGACGATCTAAATAAGGCCTTGAAGCTTGCGCGGAAGTTTAAAACCAACGTAAGACTTATCCTTGGATGTACTCAGACTCAGGTTACTGGCATTGGCACTCTACCTCGCATGTCGTTGGTCTATGTGTCGCTCTACATGGCATTTCTTGTTCTACTTGCTTTTCGGGTTATATCATATTTCAATATCACGGATGGTACCTTCATTGACGCACTTTATATTACTTCCGTTACGCTATTCACTGTGGGTTTTGGGGATATTACTCCTAAGTGGAGCGGTACTCTACTTTTCTGTGATATGTTTGGTATGTTTGGATGTATCATACCGTCTATCTACAACCATTTTGTGGGTCAGATGGCAGATTGGTTGTATCAGAGGTATCTTTCTGGTGTGCAGAGTCACCGTAGGAGACGCTCCTATCTGATGCTTTCAGTTGCTGGCATGATTTTAGTGCTTATACTTTCAGGGATGGCAGCCATTTATTATTTTGAGGGGGAGCGACTTCGTAGACAGTTTCATACTCATACCTCTGCACGAATCATTCTTGACATATTTCACCTGACCGTTATGACCATGACTACGATTGGATATGGAGATTTCGCATTTGCTACTGCACATGGGAGAGCATTCGCCTCATTATGGATTCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

357

Amino Acids

40.92

Weight (kDa)

8.17

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 188 - 258 1.2e-08 Ion channel
Ion_trans_2 PF07885 288 - 355 1.4e-07 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02510
fragaria_vesca FvH4_6g16970 FvH4_6g16980 FvH4_7g01220 FvH4_7g01220 FvH4_7g01220 FvH4_7g01240
malus_domestica MD05G1161500.v1.1
pyrus_communis pycom05g15160
rosa_chinensis RchiOBHm_Chr1g0316711 RchiOBHm_Chr1g0316801 RchiOBHm_Chr1g0316871 RchiOBHm_Chr1g0316881 RchiOBHm_Chr1g0316951 RchiOBHm_Chr3g0470991 RchiOBHm_Chr4g0402171
rosa_laevigata RLG00000001679 RLG00000001680 RLG00000007591 RLG00000009108 RLG00000024215 RLG00000024221 RLG00000030636 RLG00000030640 RLG00000030642 RLG00000030648 RLG00000030651 RLG00000030658 RLG00000030667
rosa_multiflora Rmu_sc0000839.1_g000010 Rmu_sc0002667.1_g000003 Rmu_sc0003008.1_g000023 Rmu_sc0003008.1_g000047 Rmu_sc0005604.1_g000001 Rmu_sc0005939.1_g000001 Rmu_sc0006790.1_g000011 Rmu_sc0007472.1_g000004 Rmu_sc0017515.1_g000001 Rmu_sc0019753.1_g000003 Rmu_sc0020172.1_g000001 Rmu_sc0023783.1_g000001
rosa_roxburghii Rroxscaffold_4G00330760 Rroxscaffold_4G00330800 Rroxscaffold_4G00330810 Rroxscaffold_4G00330950
rosa_rugosa Rorug01G0011200 Rorug01G0011500 Rorug01G0011600 Rorug01G0012300 Rorug01G0012300 Rorug01G0012400 Rorug01G0012500 Rorug01G0012600 Rorug01G0012600 Rorug01G0013400 Rorug01G0013400 Rorug01G0013500
rosa_samantha Rh1AG021100 Rh1AG022800 Rh1AG023300 Rh1AG023500 Rh1AG024000 Rh1AG024300 Rh1BG016000 Rh1BG016300 Rh1BG021300 Rh1BG021500 Rh1CG020300 Rh1CG022500 Rh1DG016300 Rh1DG016400 Rh1DG017300 Rh1DG017400 Rh1DG017700 Rh1DG017800 Rh1DG018200 Rh1DG019100 Rh1DG019900 Rh1DG020200 Rh1DG020800 Rh1DG021300 Rh1DG021400 Rh1DG021800 Rh3DG186400
rosa_wichuraiana Rw1G001370 Rw1G001480 Rw1G001650 Rw1G001680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 614
AccB7I CCANNNNNTGG 1 cut(s) 763
AccBSI CCGCTC 1 cut(s) 693
AccI GTMKAC 1 cut(s) 925
AccII CGCG 2 cut(s) 403, 451
AciI CCGC 2 cut(s) 451, 693
AcsI RAATTY 1 cut(s) 62
AfaI GTAC 3 cut(s) 490, 616, 697
AfiI CCNNNNNNNGG 2 cut(s) 763, 1056
AgsI TTSAA 4 cut(s) 68, 100, 442, 601
AhdI GACNNNNNGTC 1 cut(s) 47
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 7 cut(s) 29, 155, 179, 193, 362, 367, 445
AluI AGCT 7 cut(s) 29, 155, 179, 193, 362, 367, 445
Alw26I GTCTC 3 cut(s) 268, 349, 820
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 1 cut(s) 890
ApoI RAATTY 1 cut(s) 62
Asp700I GAANNNNTTC 1 cut(s) 43
Asp718I GGTACC 1 cut(s) 614
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 809, 965
AvaII GGWCC 1 cut(s) 49
BanI GGYRCC 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 902
BccI CCATC 4 cut(s) 259, 605, 766, 880
BciT130I CCWGG 1 cut(s) 378
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 3 cut(s) 268, 349, 820
BfaI CTAG 3 cut(s) 248, 278, 336
BisI GCNGC 1 cut(s) 891
BlpI GCTNAGC 2 cut(s) 156, 363
BlsI GCNGC 1 cut(s) 892
Bme1390I CCNGG 1 cut(s) 378
Bme18I GGWCC 1 cut(s) 49
BmeRI GACNNNNNGTC 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 2 cut(s) 138, 831
BpiI GAAGAC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 682, 714
Bpu10I CCTNAGC 1 cut(s) 180
Bpu1102I GCTNAGC 2 cut(s) 156, 363
BsaJI CCNNGG 1 cut(s) 481
Bsc4I CCNNNNNNNGG 2 cut(s) 763, 1056
Bse1I ACTGG 1 cut(s) 511
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 481
BseGI GGATG 5 cut(s) 373, 491, 616, 737, 891
BseLI CCNNNNNNNGG 2 cut(s) 763, 1056
BseMII CTCAG 4 cut(s) 147, 377, 506, 512
BseNI ACTGG 1 cut(s) 511
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 1 cut(s) 902
BsgI GTGCAG 3 cut(s) 830, 933, 1014
Bsh1236I CGCG 2 cut(s) 403, 451
BshFI GGCC 1 cut(s) 437
BshNI GGYRCC 1 cut(s) 614
BslI CCNNNNNNNGG 2 cut(s) 763, 1056
BsmAI GTCTC 3 cut(s) 268, 349, 820
BsmBI CGTCTC 2 cut(s) 349, 820
BsmI GAATGC 1 cut(s) 1043
BsnI GGCC 1 cut(s) 437
Bsp143I GATC 3 cut(s) 82, 370, 424
Bsp1720I GCTNAGC 2 cut(s) 156, 363
BspACI CCGC 2 cut(s) 451, 693
BspANI GGCC 1 cut(s) 437
BspCNI CTCAG 4 cut(s) 148, 376, 505, 511
BspFNI CGCG 2 cut(s) 403, 451
BspLI GGNNCC 1 cut(s) 616
BspT107I GGYRCC 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 693
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 3 cut(s) 82, 370, 424
BssT1I CCWWGG 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 5 cut(s) 661, 744, 821, 930, 982
Bst6I CTCTTC 2 cut(s) 224, 266
BstC8I GCNNGC 3 cut(s) 153, 447, 856
BstDEI CTNAG 6 cut(s) 156, 180, 363, 492, 498, 684
BstF5I GGATG 5 cut(s) 373, 491, 616, 737, 891
BstFNI CGCG 2 cut(s) 403, 451
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 3 cut(s) 85, 373, 427
BstMAI GTCTC 3 cut(s) 268, 349, 820
BstMBI GATC 3 cut(s) 82, 370, 424
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 532
BstSCI CCNGG 1 cut(s) 376
BstUI CGCG 2 cut(s) 403, 451
BstV1I GCAGC 1 cut(s) 902
BstV2I GAAGAC 1 cut(s) 46
BsuRI GGCC 1 cut(s) 437
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 5 cut(s) 373, 491, 616, 737, 891
BtsIMutI CAGTG 2 cut(s) 246, 657
Cac8I GCNNGC 3 cut(s) 153, 447, 856
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 3 cut(s) 253, 635, 837
Csp6I GTAC 3 cut(s) 489, 615, 696
CviAII CATG 6 cut(s) 161, 529, 556, 859, 991, 1034
CviQI GTAC 3 cut(s) 489, 615, 696
DdeI CTNAG 6 cut(s) 156, 180, 363, 492, 498, 684
DpnI GATC 3 cut(s) 84, 372, 426
DpnII GATC 3 cut(s) 82, 370, 424
DraI TTTAAA 1 cut(s) 460
DriI GACNNNNNGTC 1 cut(s) 47
Eam1104I CTCTTC 2 cut(s) 224, 266
Eam1105I GACNNNNNGTC 1 cut(s) 47
EarI CTCTTC 2 cut(s) 224, 266
Eco130I CCWWGG 1 cut(s) 481
Eco147I AGGCCT 1 cut(s) 437
Eco47I GGWCC 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 481
Esp3I CGTCTC 2 cut(s) 349, 820
FaeI CATG 6 cut(s) 164, 532, 559, 862, 994, 1037
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 6 cut(s) 160, 528, 555, 858, 990, 1033
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 925
Fnu4HI GCNGC 1 cut(s) 891
FokI GGATG 5 cut(s) 360, 498, 623, 744, 898
Fsp4HI GCNGC 1 cut(s) 891
FspBI CTAG 3 cut(s) 248, 278, 336
GlaI GCGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 891
HaeIII GGCC 1 cut(s) 437
HgaI GACGC 3 cut(s) 253, 635, 837
HhaI GCGC 1 cut(s) 451
Hin1II CATG 6 cut(s) 164, 532, 559, 862, 994, 1037
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 5 cut(s) 332, 496, 814, 954, 1060
HphI GGTGA 2 cut(s) 809, 965
Hpy166II GTNNAC 2 cut(s) 383, 926
Hpy188I TCNGA 7 cut(s) 61, 87, 118, 495, 771, 792, 840
Hpy188III TCNNGA 1 cut(s) 962
Hpy8I GTNNAC 2 cut(s) 383, 926
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 2 cut(s) 322, 628
HpyCH4III ACNGT 5 cut(s) 661, 744, 821, 930, 982
HpyCH4IV ACGT 3 cut(s) 103, 342, 468
HpyCH4V TGCA 4 cut(s) 151, 811, 950, 1031
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 6 cut(s) 156, 180, 363, 492, 498, 684
HpySE526I ACGT 3 cut(s) 103, 342, 468
Hsp92II CATG 6 cut(s) 164, 532, 559, 862, 994, 1037
HspAI GCGC 1 cut(s) 449
KpnI GGTACC 1 cut(s) 618
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 3 cut(s) 82, 370, 424
LmnI GCTCC 3 cut(s) 690, 836, 913
LpnPI CCDG 8 cut(s) 363, 390, 485, 492, 789, 840, 867, 989
Lsp1109I GCAGC 1 cut(s) 902
LweI GCATC 2 cut(s) 138, 831
MaeI CTAG 3 cut(s) 248, 278, 336
MaeII ACGT 3 cut(s) 103, 342, 468
MaeIII GTNAC 4 cut(s) 241, 502, 646, 815
MalI GATC 3 cut(s) 84, 372, 426
MbiI CCGCTC 1 cut(s) 693
MboI GATC 3 cut(s) 82, 370, 424
MboII GAAGA 3 cut(s) 51, 241, 283
MluCI AATT 1 cut(s) 62
MlyI GAGTC 3 cut(s) 341, 490, 823
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 8 cut(s) 100, 295, 406, 534, 786, 901, 955, 1060
MroXI GAANNNNTTC 1 cut(s) 43
MseI TTAA 3 cut(s) 288, 459, 1069
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 193
MspR9I CCNGG 1 cut(s) 378
Mva1269I GAATGC 1 cut(s) 1043
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 2 cut(s) 403, 451
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 3 cut(s) 82, 370, 424
NlaIII CATG 6 cut(s) 164, 532, 559, 862, 994, 1037
NlaIV GGNNCC 1 cut(s) 616
NmuCI GTSAC 2 cut(s) 241, 815
NspI RCATGY 1 cut(s) 532
PceI AGGCCT 1 cut(s) 437
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 1 cut(s) 1043
PdmI GAANNNNTTC 1 cut(s) 43
PfeI GAWTC 2 cut(s) 954, 1060
PflMI CCANNNNNTGG 1 cut(s) 763
PkrI GCNGC 1 cut(s) 892
PleI GAGTC 3 cut(s) 340, 490, 822
PpsI GAGTC 3 cut(s) 340, 490, 822
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 854
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 3 cut(s) 490, 616, 697
RsaNI GTAC 3 cut(s) 489, 615, 696
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 3 cut(s) 288, 459, 1069
SatI GCNGC 1 cut(s) 891
Sau3AI GATC 3 cut(s) 82, 370, 424
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 3 cut(s) 341, 490, 823
ScrFI CCNGG 1 cut(s) 378
SfaNI GCATC 2 cut(s) 138, 831
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 378
Sse9I AATT 1 cut(s) 62
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 2 cut(s) 451, 693
SspMI CTAG 3 cut(s) 248, 278, 336
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 376
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 5 cut(s) 661, 744, 821, 930, 982
TaiI ACGT 3 cut(s) 106, 345, 471
TaqI TCGA 2 cut(s) 326, 420
TaqII GACCGA 1 cut(s) 37
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 488
TfiI GAWTC 2 cut(s) 954, 1060
Tru1I TTAA 3 cut(s) 288, 459, 1069
Tru9I TTAA 3 cut(s) 288, 459, 1069
TscAI CASTG 2 cut(s) 246, 664
TseFI GTSAC 2 cut(s) 241, 815
TseI GCWGC 1 cut(s) 890
Tsp45I GTSAC 2 cut(s) 241, 815
TspDTI ATGAA 3 cut(s) 180, 610, 923
TspGWI ACGGA 3 cut(s) 22, 623, 634
TspRI CASTG 2 cut(s) 246, 664
Van91I CCANNNNNTGG 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 925
XmnI GAANNNNTTC 1 cut(s) 43
XspI CTAG 3 cut(s) 248, 278, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.