Threonine dehydratase biosynthetic
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 363 |
| Acc65I | GGTACC | 1 cut(s) | 5 |
| AccB1I | GGYRCC | 1 cut(s) | 5 |
| AccI | GTMKAC | 1 cut(s) | 1332 |
| AccII | CGCG | 1 cut(s) | 1142 |
| AciI | CCGC | 2 cut(s) | 146, 1140 |
| AclWI | GGATC | 1 cut(s) | 210 |
| AcsI | RAATTY | 2 cut(s) | 891, 1173 |
| AcuI | CTGAAG | 2 cut(s) | 966, 1371 |
| AcvI | CACGTG | 1 cut(s) | 791 |
| AfaI | GTAC | 7 cut(s) | 7, 248, 325, 469, 527, 689, 1170 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 288, 734 |
| AflIII | ACRYGT | 1 cut(s) | 790 |
| AgsI | TTSAA | 8 cut(s) | 85, 461, 549, 603, 734, 886, 897, 1478 |
| AjnI | CCWGG | 1 cut(s) | 579 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1260, 1292 |
| AluBI | AGCT | 5 cut(s) | 58, 344, 615, 824, 1490 |
| AluI | AGCT | 5 cut(s) | 58, 344, 615, 824, 1490 |
| Alw21I | GWGCWC | 2 cut(s) | 364, 1399 |
| Alw26I | GTCTC | 2 cut(s) | 258, 877 |
| Alw44I | GTGCAC | 1 cut(s) | 360 |
| AlwI | GGATC | 1 cut(s) | 210 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 515 |
| Ama87I | CYCGRG | 2 cut(s) | 59, 1440 |
| AoxI | GGCC | 1 cut(s) | 448 |
| ApaLI | GTGCAC | 1 cut(s) | 360 |
| ApeKI | GCWGC | 3 cut(s) | 344, 509, 670 |
| ApoI | RAATTY | 2 cut(s) | 891, 1173 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1062 |
| Asp718I | GGTACC | 1 cut(s) | 5 |
| AspLEI | GCGC | 2 cut(s) | 105, 1144 |
| AspS9I | GGNCC | 2 cut(s) | 448, 577 |
| AsuHPI | GGTGA | 6 cut(s) | 29, 473, 612, 1124, 1292, 1505 |
| AvaI | CYCGRG | 2 cut(s) | 59, 1440 |
| AvaII | GGWCC | 1 cut(s) | 577 |
| BaeGI | GKGCMC | 1 cut(s) | 364 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 459, 492 |
| BanI | GGYRCC | 1 cut(s) | 5 |
| BbrPI | CACGTG | 1 cut(s) | 791 |
| BbsI | GAAGAC | 2 cut(s) | 413, 954 |
| Bbv12I | GWGCWC | 2 cut(s) | 364, 1399 |
| BbvI | GCAGC | 3 cut(s) | 331, 521, 682 |
| BccI | CCATC | 2 cut(s) | 17, 1109 |
| BceAI | ACGGC | 2 cut(s) | 665, 1304 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1073, 1107, 1421, 1455 |
| BciT130I | CCWGG | 1 cut(s) | 581 |
| BclI | TGATCA | 1 cut(s) | 1516 |
| BcoDI | GTCTC | 2 cut(s) | 258, 877 |
| BfaI | CTAG | 1 cut(s) | 185 |
| BfmI | CTRYAG | 1 cut(s) | 969 |
| BfuAI | ACCTGC | 1 cut(s) | 363 |
| BisI | GCNGC | 4 cut(s) | 345, 510, 671, 1140 |
| BlsI | GCNGC | 4 cut(s) | 346, 511, 672, 1141 |
| Bme1390I | CCNGG | 1 cut(s) | 581 |
| Bme18I | GGWCC | 1 cut(s) | 577 |
| BmeT110I | CYCGRG | 2 cut(s) | 59, 1440 |
| BmgT120I | GGNCC | 2 cut(s) | 448, 577 |
| BmiI | GGNNCC | 3 cut(s) | 7, 578, 585 |
| BmrFI | CCNGG | 1 cut(s) | 581 |
| BmsI | GCATC | 7 cut(s) | 168, 178, 356, 496, 583, 942, 1306 |
| BpiI | GAAGAC | 2 cut(s) | 413, 954 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 857, 889 |
| BpuEI | CTTGAG | 1 cut(s) | 506 |
| BsaAI | YACGTR | 3 cut(s) | 791, 991, 1147 |
| BsaJI | CCNNGG | 1 cut(s) | 777 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 288, 734 |
| Bse1I | ACTGG | 2 cut(s) | 1106, 1384 |
| Bse3DI | GCAATG | 2 cut(s) | 423, 714 |
| BseBI | CCWGG | 1 cut(s) | 581 |
| BseDI | CCNNGG | 1 cut(s) | 777 |
| BseGI | GGATG | 2 cut(s) | 904, 933 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 288, 734 |
| BseMI | GCAATG | 2 cut(s) | 423, 714 |
| BseMII | CTCAG | 1 cut(s) | 443 |
| BseNI | ACTGG | 2 cut(s) | 1106, 1384 |
| BseSI | GKGCMC | 1 cut(s) | 364 |
| BseXI | GCAGC | 3 cut(s) | 331, 521, 682 |
| BseYI | CCCAGC | 1 cut(s) | 1409 |
| Bsh1236I | CGCG | 1 cut(s) | 1142 |
| BshFI | GGCC | 1 cut(s) | 450 |
| BshNI | GGYRCC | 1 cut(s) | 5 |
| BsiHKAI | GWGCWC | 2 cut(s) | 364, 1399 |
| BsiHKCI | CYCGRG | 2 cut(s) | 59, 1440 |
| BslFI | GGGAC | 3 cut(s) | 590, 715, 1045 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 288, 734 |
| BsmAI | GTCTC | 2 cut(s) | 258, 877 |
| BsmFI | GGGAC | 3 cut(s) | 590, 715, 1045 |
| BsmI | GAATGC | 1 cut(s) | 1292 |
| BsnI | GGCC | 1 cut(s) | 450 |
| BsoBI | CYCGRG | 2 cut(s) | 59, 1440 |
| Bsp1286I | GDGCHC | 2 cut(s) | 364, 1399 |
| Bsp1407I | TGTACA | 1 cut(s) | 246 |
| Bsp143I | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| BspACI | CCGC | 2 cut(s) | 146, 1140 |
| BspANI | GGCC | 1 cut(s) | 450 |
| BspCNI | CTCAG | 1 cut(s) | 442 |
| BspFNI | CGCG | 1 cut(s) | 1142 |
| BspLI | GGNNCC | 3 cut(s) | 7, 578, 585 |
| BspMI | ACCTGC | 1 cut(s) | 363 |
| BspPI | GGATC | 1 cut(s) | 210 |
| BspT107I | GGYRCC | 1 cut(s) | 5 |
| BsrDI | GCAATG | 2 cut(s) | 423, 714 |
| BsrGI | TGTACA | 1 cut(s) | 246 |
| BsrI | ACTGG | 2 cut(s) | 1106, 1384 |
| BssECI | CCNNGG | 1 cut(s) | 777 |
| BssMI | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| BssT1I | CCWWGG | 1 cut(s) | 777 |
| Bst2UI | CCWGG | 1 cut(s) | 581 |
| Bst4CI | ACNGT | 3 cut(s) | 328, 879, 1193 |
| Bst6I | CTCTTC | 3 cut(s) | 550, 1473, 1491 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 515, 794, 803 |
| BstAUI | TGTACA | 1 cut(s) | 246 |
| BstBAI | YACGTR | 3 cut(s) | 791, 991, 1147 |
| BstC8I | GCNNGC | 4 cut(s) | 360, 452, 774, 1430 |
| BstDEI | CTNAG | 1 cut(s) | 429 |
| BstEII | GGTNACC | 1 cut(s) | 1118 |
| BstF5I | GGATG | 2 cut(s) | 904, 933 |
| BstFNI | CGCG | 1 cut(s) | 1142 |
| BstHHI | GCGC | 2 cut(s) | 105, 1144 |
| BstKTI | GATC | 5 cut(s) | 205, 976, 1050, 1087, 1519 |
| BstMAI | GTCTC | 2 cut(s) | 258, 877 |
| BstMBI | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 515, 794, 803, 1136 |
| BstNI | CCWGG | 1 cut(s) | 581 |
| BstNSI | RCATGY | 3 cut(s) | 600, 759, 872 |
| BstPI | GGTNACC | 1 cut(s) | 1118 |
| BstSCI | CCNGG | 1 cut(s) | 579 |
| BstSFI | CTRYAG | 1 cut(s) | 969 |
| BstSLI | GKGCMC | 1 cut(s) | 364 |
| BstUI | CGCG | 1 cut(s) | 1142 |
| BstV1I | GCAGC | 3 cut(s) | 331, 521, 682 |
| BstV2I | GAAGAC | 2 cut(s) | 413, 954 |
| BsuRI | GGCC | 1 cut(s) | 450 |
| BtsCI | GGATG | 2 cut(s) | 904, 933 |
| BtsI | GCAGTG | 1 cut(s) | 513 |
| BtsIMutI | CAGTG | 3 cut(s) | 513, 1008, 1377 |
| BveI | ACCTGC | 1 cut(s) | 363 |
| Cac8I | GCNNGC | 4 cut(s) | 360, 452, 774, 1430 |
| CaiI | CAGNNNCTG | 1 cut(s) | 515 |
| CfoI | GCGC | 2 cut(s) | 105, 1144 |
| Cfr13I | GGNCC | 2 cut(s) | 448, 577 |
| Csp6I | GTAC | 7 cut(s) | 6, 247, 324, 468, 526, 688, 1169 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 180, 215 |
| CviQI | GTAC | 7 cut(s) | 6, 247, 324, 468, 526, 688, 1169 |
| DdeI | CTNAG | 1 cut(s) | 429 |
| DpnI | GATC | 5 cut(s) | 204, 975, 1049, 1086, 1518 |
| DpnII | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| Eam1104I | CTCTTC | 3 cut(s) | 550, 1473, 1491 |
| EarI | CTCTTC | 3 cut(s) | 550, 1473, 1491 |
| Eco130I | CCWWGG | 1 cut(s) | 777 |
| Eco47I | GGWCC | 1 cut(s) | 577 |
| Eco57I | CTGAAG | 2 cut(s) | 966, 1371 |
| Eco72I | CACGTG | 1 cut(s) | 791 |
| Eco88I | CYCGRG | 2 cut(s) | 59, 1440 |
| Eco91I | GGTNACC | 1 cut(s) | 1118 |
| EcoO109I | RGGNCCY | 1 cut(s) | 577 |
| EcoO65I | GGTNACC | 1 cut(s) | 1118 |
| EcoRII | CCWGG | 1 cut(s) | 579 |
| EcoT14I | CCWWGG | 1 cut(s) | 777 |
| EcoT22I | ATGCAT | 2 cut(s) | 161, 598 |
| ErhI | CCWWGG | 1 cut(s) | 777 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 427, 459 |
| FaqI | GGGAC | 3 cut(s) | 590, 715, 1045 |
| FauNDI | CATATG | 1 cut(s) | 1298 |
| FbaI | TGATCA | 1 cut(s) | 1516 |
| FblI | GTMKAC | 1 cut(s) | 1332 |
| Fnu4HI | GCNGC | 4 cut(s) | 345, 510, 671, 1140 |
| FokI | GGATG | 2 cut(s) | 891, 920 |
| Fsp4HI | GCNGC | 4 cut(s) | 345, 510, 671, 1140 |
| FspBI | CTAG | 1 cut(s) | 185 |
| GlaI | GCGC | 2 cut(s) | 104, 1143 |
| GluI | GCNGC | 4 cut(s) | 345, 510, 671, 1140 |
| GsaI | CCCAGC | 1 cut(s) | 1413 |
| HaeIII | GGCC | 1 cut(s) | 450 |
| HhaI | GCGC | 2 cut(s) | 105, 1144 |
| Hin6I | GCGC | 2 cut(s) | 103, 1142 |
| HinP1I | GCGC | 2 cut(s) | 103, 1142 |
| HincII | GTYRAC | 1 cut(s) | 217 |
| HindII | GTYRAC | 1 cut(s) | 217 |
| HinfI | GANTC | 3 cut(s) | 165, 886, 941 |
| HpaI | GTTAAC | 1 cut(s) | 217 |
| HphI | GGTGA | 6 cut(s) | 29, 473, 612, 1124, 1292, 1505 |
| Hpy166II | GTNNAC | 5 cut(s) | 20, 217, 249, 362, 1333 |
| Hpy188I | TCNGA | 7 cut(s) | 164, 767, 862, 938, 946, 1198, 1306 |
| Hpy8I | GTNNAC | 5 cut(s) | 20, 217, 249, 362, 1333 |
| Hpy99I | CGWCG | 1 cut(s) | 688 |
| HpyAV | CCTTC | 3 cut(s) | 391, 728, 736 |
| HpyCH4III | ACNGT | 3 cut(s) | 328, 879, 1193 |
| HpyCH4IV | ACGT | 3 cut(s) | 790, 990, 1146 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 515, 794, 803, 1136 |
| HpyF3I | CTNAG | 1 cut(s) | 429 |
| HpySE526I | ACGT | 3 cut(s) | 790, 990, 1146 |
| HspAI | GCGC | 2 cut(s) | 103, 1142 |
| KpnI | GGTACC | 1 cut(s) | 9 |
| Ksp22I | TGATCA | 1 cut(s) | 1516 |
| KspAI | GTTAAC | 1 cut(s) | 217 |
| Kzo9I | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| LmnI | GCTCC | 1 cut(s) | 829 |
| Lsp1109I | GCAGC | 3 cut(s) | 331, 521, 682 |
| LweI | GCATC | 7 cut(s) | 168, 178, 356, 496, 583, 942, 1306 |
| MaeI | CTAG | 1 cut(s) | 185 |
| MaeII | ACGT | 3 cut(s) | 790, 990, 1146 |
| MaeIII | GTNAC | 3 cut(s) | 475, 1001, 1118 |
| MalI | GATC | 5 cut(s) | 204, 975, 1049, 1086, 1518 |
| MboI | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| MfeI | CAATTG | 2 cut(s) | 729, 1035 |
| MhlI | GDGCHC | 2 cut(s) | 364, 1399 |
| MlyI | GAGTC | 1 cut(s) | 950 |
| MmeI | TCCRAC | 2 cut(s) | 353, 1224 |
| Mph1103I | ATGCAT | 2 cut(s) | 161, 598 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1062 |
| MseI | TTAA | 3 cut(s) | 216, 813, 1224 |
| MslI | CAYNNNNRTG | 1 cut(s) | 909 |
| MspR9I | CCNGG | 1 cut(s) | 581 |
| MunI | CAATTG | 2 cut(s) | 729, 1035 |
| Mva1269I | GAATGC | 1 cut(s) | 1292 |
| MvaI | CCWGG | 1 cut(s) | 581 |
| MvnI | CGCG | 1 cut(s) | 1142 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 515, 794, 803, 1136 |
| NdeI | CATATG | 1 cut(s) | 1298 |
| NdeII | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| NlaIV | GGNNCC | 3 cut(s) | 7, 578, 585 |
| NmuCI | GTSAC | 1 cut(s) | 1001 |
| NsiI | ATGCAT | 2 cut(s) | 161, 598 |
| NspI | RCATGY | 3 cut(s) | 600, 759, 872 |
| PaeR7I | CTCGAG | 2 cut(s) | 59, 1440 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1447 |
| PctI | GAATGC | 1 cut(s) | 1292 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1062 |
| PfeI | GAWTC | 2 cut(s) | 165, 886 |
| PkrI | GCNGC | 4 cut(s) | 346, 511, 672, 1141 |
| PleI | GAGTC | 1 cut(s) | 949 |
| PmaCI | CACGTG | 1 cut(s) | 791 |
| PmlI | CACGTG | 1 cut(s) | 791 |
| PpsI | GAGTC | 1 cut(s) | 949 |
| Ppu21I | YACGTR | 3 cut(s) | 791, 991, 1147 |
| PpuMI | RGGWCCY | 1 cut(s) | 577 |
| Psp5II | RGGWCCY | 1 cut(s) | 577 |
| Psp6I | CCWGG | 1 cut(s) | 579 |
| PspCI | CACGTG | 1 cut(s) | 791 |
| PspEI | GGTNACC | 1 cut(s) | 1118 |
| PspFI | CCCAGC | 1 cut(s) | 1409 |
| PspGI | CCWGG | 1 cut(s) | 579 |
| PspN4I | GGNNCC | 3 cut(s) | 7, 578, 585 |
| PspPI | GGNCC | 2 cut(s) | 448, 577 |
| PspPPI | RGGWCCY | 1 cut(s) | 577 |
| PstNI | CAGNNNCTG | 1 cut(s) | 515 |
| RsaI | GTAC | 7 cut(s) | 7, 248, 325, 469, 527, 689, 1170 |
| RsaNI | GTAC | 7 cut(s) | 6, 247, 324, 468, 526, 688, 1169 |
| RseI | CAYNNNNRTG | 1 cut(s) | 909 |
| SaqAI | TTAA | 3 cut(s) | 216, 813, 1224 |
| SatI | GCNGC | 4 cut(s) | 345, 510, 671, 1140 |
| Sau3AI | GATC | 5 cut(s) | 202, 973, 1047, 1084, 1516 |
| Sau96I | GGNCC | 2 cut(s) | 448, 577 |
| SchI | GAGTC | 1 cut(s) | 950 |
| ScrFI | CCNGG | 1 cut(s) | 581 |
| SduI | GDGCHC | 2 cut(s) | 364, 1399 |
| SfaNI | GCATC | 7 cut(s) | 168, 178, 356, 496, 583, 942, 1306 |
| SfcI | CTRYAG | 1 cut(s) | 969 |
| Sfr274I | CTCGAG | 2 cut(s) | 59, 1440 |
| SinI | GGWCC | 1 cut(s) | 577 |
| SlaI | CTCGAG | 2 cut(s) | 59, 1440 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 909 |
| SmlI | CTYRAG | 3 cut(s) | 59, 521, 1440 |
| SmoI | CTYRAG | 3 cut(s) | 59, 521, 1440 |
| SsiI | CCGC | 2 cut(s) | 146, 1140 |
| SspI | AATATT | 1 cut(s) | 1436 |
| SspMI | CTAG | 1 cut(s) | 185 |
| StyD4I | CCNGG | 1 cut(s) | 579 |
| StyI | CCWWGG | 1 cut(s) | 777 |
| TaaI | ACNGT | 3 cut(s) | 328, 879, 1193 |
| TaiI | ACGT | 3 cut(s) | 793, 993, 1149 |
| TaqI | TCGA | 6 cut(s) | 60, 205, 340, 889, 1066, 1441 |
| TaqII | GACCGA | 1 cut(s) | 223 |
| TatI | WGTACW | 2 cut(s) | 246, 525 |
| TauI | GCSGC | 1 cut(s) | 1142 |
| TfiI | GAWTC | 2 cut(s) | 165, 886 |
| Tru1I | TTAA | 3 cut(s) | 216, 813, 1224 |
| Tru9I | TTAA | 3 cut(s) | 216, 813, 1224 |
| TscAI | CASTG | 3 cut(s) | 520, 1015, 1384 |
| TseFI | GTSAC | 1 cut(s) | 1001 |
| TseI | GCWGC | 3 cut(s) | 344, 509, 670 |
| Tsp45I | GTSAC | 1 cut(s) | 1001 |
| TspDTI | ATGAA | 3 cut(s) | 703, 1071, 1521 |
| TspGWI | ACGGA | 1 cut(s) | 548 |
| TspRI | CASTG | 3 cut(s) | 520, 1015, 1384 |
| VneI | GTGCAC | 1 cut(s) | 360 |
| VpaK11BI | GGWCC | 1 cut(s) | 577 |
| XapI | RAATTY | 2 cut(s) | 891, 1173 |
| XbaI | TCTAGA | 1 cut(s) | 184 |
| XceI | RCATGY | 3 cut(s) | 600, 759, 872 |
| XhoI | CTCGAG | 2 cut(s) | 59, 1440 |
| XmiI | GTMKAC | 1 cut(s) | 1332 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1062 |
| XspI | CTAG | 1 cut(s) | 185 |
| Zsp2I | ATGCAT | 2 cut(s) | 161, 598 |