Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Reverse (-)

26962020 .. 26963681

Gene Structure 1662 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1139100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1233 bp

ATGTCTCGGAACCAACTCACTGGCCAAATACCTGACGGAATACCCGCCATGGAAACCCTTACAACTTTGGATCTCTCTTATAACAATTTCACCGGTAGAATCCCAACCAGGGGTCATTTTCTGGACATTGCCTCGTTTCGTGGGAACCCCTATCTCTGTACCAACGTCTCACGCCCGTGTTCCTTCATTAACAAGAAATCTCAAGACCACAATGTGTTTGGTTCCTCCAGGCTCGGTCTTATGATCATGGTTGGACTACCTGCGGTTCTCGGTTCCCTACTTATCCTACTTCTGGTATTCCTACTGATAAAGGTTTGTACAGGAAGAAATATTCAAAAATCTAGTGGTTGGAGGTTCACATTGTTCCAGCAGCTCGACCTCAAAGTCGAGGACTTGCTACAGTGCTTAAAGCAAGAGAACATTATAGGCAAAGGTGGTGCTGGGGTTGTCTACCGGGCAACCATGCCAAGCGGCCTCGTCATAGCAATCAAACAGCTAGTAGGATCATCAAGTAATACAGCAAAAACGGATCATGGATTTGCAGCGGAAATTAAGACGCTGGGACGAATCAAACACCGAAACATAGTGAGGGTTTTGGGATACATGTCGAACAATAAGGACTCTAATTTGTTGTTGTACGAGTACATGCCTAATGGTAGCTTGGGAAAAATATTGCATGGGGAAAATGGAGGGGAGTTGCAGTGGGAGAGGAGGTATAAAATTGCAGTGGAGGCTGCAAAGGGATTGTGTTATCTGCACCGTGACTGTTCACCCCTGATTATACACAGAGATGTCAAGTCCCATAACATCTTGTTGGATTCAAACTTTGAGGCTCATGTTGCTGATTTTGGGCTCGCCAAGTACTTTCATGGAGTAGCAGCTGATTGCATGTCTTCAGTTGCTGGTTCCTTCGGTTACATCGCCCCAGTACGGTTATACACTGAAAGTGACGTGTACAGTTTTGGTGTGGTTTTGCTGGAGCTCATAACAGGGAGGAAAGCAGTGATGAACTATGAAGACGCTATAAACATAGTGGGTTGGGTGAGGAAAACTACCAAGTCGTTGTCCAGTTCATCATCGGTACTAGCAGTGGTGGATCCAAATCTCGCCGGATACCCCTTGGCGGCTGTCATACATTTGTTCAAACTGGCAATGCTGCGTGTCGACAACGACAGCTCTGCAAGACCTACGATGAGGGAAGTAGTTCACCTGATCAGTAATCCTCCTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

411

Amino Acids

45.07

Weight (kDa)

9.26

Isoelectric Point (pI)

39.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	137 - 402	1.1e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	137 - 400	1.4e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	81
AasI	GACNNNNNNGTC	1 cut(s)	383
Acc36I	ACCTGC	1 cut(s)	268
AccI	GTMKAC	2 cut(s)	450, 1164
AciI	CCGC	5 cut(s)	45, 263, 471, 545, 1124
AclWI	GGATC	5 cut(s)	78, 511, 537, 1091, 1104
AcoI	YGGCCR	1 cut(s)	22
AcuI	CTGAAG	1 cut(s)	879
AdeI	CACNNNGTG	1 cut(s)	214
AfaI	GTAC	8 cut(s)	160, 319, 638, 644, 863, 930, 956, 1083
AfiI	CCNNNNNNNGG	5 cut(s)	109, 110, 292, 930, 1123
AflIII	ACRYGT	2 cut(s)	603, 951
AgeI	ACCGGT	1 cut(s)	92
AgsI	TTSAA	3 cut(s)	335, 822, 1144
AjiI	CACGTC	1 cut(s)	952
AjnI	CCWGG	2 cut(s)	107, 227
AleI	CACNNNNGTG	1 cut(s)	175
AluBI	AGCT	6 cut(s)	373, 496, 660, 881, 982, 1176
AluI	AGCT	6 cut(s)	373, 496, 660, 881, 982, 1176
Alw21I	GWGCWC	1 cut(s)	984
Alw26I	GTCTC	2 cut(s)	9, 172
AlwI	GGATC	5 cut(s)	78, 511, 537, 1091, 1104
AlwNI	CAGNNNCTG	1 cut(s)	902
AoxI	GGCC	2 cut(s)	22, 472
ApeKI	GCWGC	5 cut(s)	370, 542, 734, 878, 1156
AsiGI	ACCGGT	1 cut(s)	92
Asp700I	GAANNNNTTC	1 cut(s)	1203
AsuC2I	CCSGG	1 cut(s)	455
AsuHPI	GGTGA	4 cut(s)	82, 762, 1054, 1199
BalI	TGGCCA	1 cut(s)	24
BamHI	GGATCC	1 cut(s)	1096
BanII	GRGCYC	2 cut(s)	855, 984
BbsI	GAAGAC	2 cut(s)	885, 1023
Bbv12I	GWGCWC	1 cut(s)	984
BbvI	GCAGC	5 cut(s)	382, 554, 721, 890, 1143
BcgI	CGANNNNNNTGC	2 cut(s)	1160, 1194
BciT130I	CCWGG	2 cut(s)	109, 229
BciVI	GTATCC	2 cut(s)	593, 1106
BclI	TGATCA	2 cut(s)	243, 1212
BcnI	CCSGG	1 cut(s)	455
BcoDI	GTCTC	2 cut(s)	9, 172
BfaI	CTAG	3 cut(s)	342, 497, 1085
BfmI	CTRYAG	1 cut(s)	398
BfuAI	ACCTGC	1 cut(s)	268
BfuI	GTATCC	2 cut(s)	593, 1106
BisI	GCNGC	7 cut(s)	371, 472, 543, 735, 879, 1125, 1157
BlsI	GCNGC	7 cut(s)	372, 473, 544, 736, 880, 1126, 1158
BmcAI	AGTACT	1 cut(s)	863
Bme1390I	CCNGG	3 cut(s)	109, 229, 455
BmgBI	CACGTC	1 cut(s)	952
BmiI	GGNNCC	6 cut(s)	11, 146, 223, 274, 907, 1098
BmrFI	CCNGG	3 cut(s)	109, 229, 455
BmrI	ACTGGG	1 cut(s)	920
BmuI	ACTGGG	1 cut(s)	920
BpiI	GAAGAC	2 cut(s)	885, 1023
BpmI	CTGGAG	2 cut(s)	211, 998
BpuEI	CTTGAG	1 cut(s)	186
BpuMI	CCSGG	1 cut(s)	455
BsaBI	GATNNNNATC	1 cut(s)	1101
BsaJI	CCNNGG	3 cut(s)	48, 108, 1119
BsaWI	WCCGGW	1 cut(s)	92
BsaXI	ACNNNNNCTCC	4 cut(s)	686, 698, 716, 728
Bsc4I	CCNNNNNNNGG	5 cut(s)	109, 110, 292, 930, 1123
Bse118I	RCCGGY	1 cut(s)	92
Bse1I	ACTGG	4 cut(s)	25, 926, 1068, 1152
Bse3DI	GCAATG	2 cut(s)	126, 1158
Bse8I	GATNNNNATC	1 cut(s)	1101
BseBI	CCWGG	2 cut(s)	109, 229
BseDI	CCNNGG	3 cut(s)	48, 108, 1119
BseJI	GATNNNNATC	1 cut(s)	1101
BseLI	CCNNNNNNNGG	5 cut(s)	109, 110, 292, 930, 1123
BseMI	GCAATG	2 cut(s)	126, 1158
BseNI	ACTGG	4 cut(s)	25, 926, 1068, 1152
BseRI	GAGGAG	1 cut(s)	724
BseXI	GCAGC	5 cut(s)	382, 554, 721, 890, 1143
BseYI	CCCAGC	2 cut(s)	440, 559
BsgI	GTGCAG	1 cut(s)	740
BshFI	GGCC	2 cut(s)	24, 474
BshTI	ACCGGT	1 cut(s)	92
BsiHKAI	GWGCWC	1 cut(s)	984
BsiSI	CCGG	3 cut(s)	93, 454, 1110
BslFI	GGGAC	2 cut(s)	576, 784
BslI	CCNNNNNNNGG	5 cut(s)	109, 110, 292, 930, 1123
BsmAI	GTCTC	2 cut(s)	9, 172
BsmBI	CGTCTC	1 cut(s)	172
BsmFI	GGGAC	2 cut(s)	576, 784
BsnI	GGCC	2 cut(s)	24, 474
Bsp1286I	GDGCHC	2 cut(s)	855, 984
Bsp1407I	TGTACA	2 cut(s)	317, 954
Bsp143I	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
Bsp19I	CCATGG	1 cut(s)	48
BspACI	CCGC	5 cut(s)	45, 263, 471, 545, 1124
BspANI	GGCC	2 cut(s)	24, 474
BspLI	GGNNCC	6 cut(s)	11, 146, 223, 274, 907, 1098
BspMI	ACCTGC	1 cut(s)	268
BspPI	GGATC	5 cut(s)	78, 511, 537, 1091, 1104
BsrDI	GCAATG	2 cut(s)	126, 1158
BsrFI	RCCGGY	1 cut(s)	92
BsrGI	TGTACA	2 cut(s)	317, 954
BsrI	ACTGG	4 cut(s)	25, 926, 1068, 1152
BssAI	RCCGGY	1 cut(s)	92
BssECI	CCNNGG	3 cut(s)	48, 108, 1119
BssMI	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
BssT1I	CCWWGG	2 cut(s)	48, 1119
Bst2UI	CCWGG	2 cut(s)	109, 229
Bst4CI	ACNGT	5 cut(s)	402, 761, 767, 933, 959
BstAUI	TGTACA	2 cut(s)	317, 954
BstC8I	GCNNGC	1 cut(s)	855
BstDSI	CCRYGG	1 cut(s)	48
BstKTI	GATC	6 cut(s)	73, 246, 506, 532, 1099, 1215
BstMAI	GTCTC	2 cut(s)	9, 172
BstMBI	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
BstMWI	GCNNNNNNNGC	2 cut(s)	731, 839
BstNI	CCWGG	2 cut(s)	109, 229
BstNSI	RCATGY	3 cut(s)	607, 649, 892
BstSCI	CCNGG	3 cut(s)	107, 227, 453
BstSFI	CTRYAG	1 cut(s)	398
BstV1I	GCAGC	5 cut(s)	382, 554, 721, 890, 1143
BstV2I	GAAGAC	2 cut(s)	885, 1023
BstX2I	RGATCY	2 cut(s)	70, 1096
BstXI	CCANNNNNNTGG	1 cut(s)	20
BstYI	RGATCY	2 cut(s)	70, 1096
BsuI	GTATCC	2 cut(s)	593, 1106
BsuRI	GGCC	2 cut(s)	24, 474
BtgI	CCRYGG	1 cut(s)	48
BtgZI	GCGATG	1 cut(s)	904
BtrI	CACGTC	1 cut(s)	952
BtsI	GCAGTG	4 cut(s)	707, 732, 1008, 1095
BtsIMutI	CAGTG	7 cut(s)	18, 407, 707, 732, 939, 1008, 1095
BveI	ACCTGC	1 cut(s)	268
Cac8I	GCNNGC	1 cut(s)	855
CaiI	CAGNNNCTG	1 cut(s)	902
Cfr10I	RCCGGY	1 cut(s)	92
CseI	GACGC	2 cut(s)	565, 1028
Csp6I	GTAC	8 cut(s)	159, 318, 637, 643, 862, 929, 955, 1082
CspAI	ACCGGT	1 cut(s)	92
CviQI	GTAC	8 cut(s)	159, 318, 637, 643, 862, 929, 955, 1082
DpnI	GATC	6 cut(s)	72, 245, 505, 531, 1098, 1214
DpnII	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
DraIII	CACNNNGTG	1 cut(s)	214
DrdI	GACNNNNNNGTC	1 cut(s)	383
DseDI	GACNNNNNNGTC	1 cut(s)	383
EaeI	YGGCCR	1 cut(s)	22
Ecl136II	GAGCTC	1 cut(s)	982
Eco130I	CCWWGG	2 cut(s)	48, 1119
Eco24I	GRGCYC	2 cut(s)	855, 984
Eco53kI	GAGCTC	1 cut(s)	982
Eco57I	CTGAAG	1 cut(s)	879
EcoICRI	GAGCTC	1 cut(s)	982
EcoRII	CCWGG	2 cut(s)	107, 227
EcoT14I	CCWWGG	2 cut(s)	48, 1119
EcoT38I	GRGCYC	2 cut(s)	855, 984
ErhI	CCWWGG	2 cut(s)	48, 1119
Esp3I	CGTCTC	1 cut(s)	172
FaqI	GGGAC	2 cut(s)	576, 784
FauI	CCCGC	1 cut(s)	52
FbaI	TGATCA	2 cut(s)	243, 1212
FblI	GTMKAC	2 cut(s)	450, 1164
Fnu4HI	GCNGC	7 cut(s)	371, 472, 543, 735, 879, 1125, 1157
FriOI	GRGCYC	2 cut(s)	855, 984
Fsp4HI	GCNGC	7 cut(s)	371, 472, 543, 735, 879, 1125, 1157
FspBI	CTAG	3 cut(s)	342, 497, 1085
GluI	GCNGC	7 cut(s)	371, 472, 543, 735, 879, 1125, 1157
GsaI	CCCAGC	2 cut(s)	444, 563
GsuI	CTGGAG	2 cut(s)	211, 998
HaeIII	GGCC	2 cut(s)	24, 474
HapII	CCGG	3 cut(s)	93, 454, 1110
HgaI	GACGC	2 cut(s)	565, 1028
HincII	GTYRAC	1 cut(s)	1165
HindII	GTYRAC	1 cut(s)	1165
HinfI	GANTC	4 cut(s)	99, 567, 620, 818
HpaII	CCGG	3 cut(s)	93, 454, 1110
HphI	GGTGA	4 cut(s)	82, 762, 1054, 1199
Hpy166II	GTNNAC	6 cut(s)	357, 451, 770, 955, 1165, 1207
Hpy188I	TCNGA	1 cut(s)	9
Hpy188III	TCNNGA	2 cut(s)	122, 203
Hpy8I	GTNNAC	6 cut(s)	357, 451, 770, 955, 1165, 1207
HpyAV	CCTTC	2 cut(s)	193, 919
HpyCH4III	ACNGT	5 cut(s)	402, 761, 767, 933, 959
HpyCH4IV	ACGT	2 cut(s)	165, 951
HpyCH4V	TGCA	8 cut(s)	542, 676, 700, 725, 737, 757, 888, 1181
HpyF10VI	GCNNNNNNNGC	2 cut(s)	731, 839
HpySE526I	ACGT	2 cut(s)	165, 951
Ksp22I	TGATCA	2 cut(s)	243, 1212
Kzo9I	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
LmnI	GCTCC	1 cut(s)	979
Lsp1109I	GCAGC	5 cut(s)	382, 554, 721, 890, 1143
MaeI	CTAG	3 cut(s)	342, 497, 1085
MaeII	ACGT	2 cut(s)	165, 951
MaeIII	GTNAC	3 cut(s)	761, 914, 947
MalI	GATC	6 cut(s)	72, 245, 505, 531, 1098, 1214
MboI	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
MboII	GAAGA	3 cut(s)	336, 885, 1028
MflI	RGATCY	2 cut(s)	70, 1096
MhlI	GDGCHC	2 cut(s)	855, 984
MlsI	TGGCCA	1 cut(s)	24
MluCI	AATT	4 cut(s)	85, 549, 625, 720
MluNI	TGGCCA	1 cut(s)	24
MlyI	GAGTC	1 cut(s)	614
MmeI	TCCRAC	3 cut(s)	232, 329, 795
Mox20I	TGGCCA	1 cut(s)	24
MroXI	GAANNNNTTC	1 cut(s)	1203
MscI	TGGCCA	1 cut(s)	24
MseI	TTAA	3 cut(s)	189, 407, 552
MslI	CAYNNNNRTG	2 cut(s)	175, 789
Msp20I	TGGCCA	1 cut(s)	24
MspA1I	CMGCKG	2 cut(s)	545, 881
MspI	CCGG	3 cut(s)	93, 454, 1110
MspR9I	CCNGG	3 cut(s)	109, 229, 455
MvaI	CCWGG	2 cut(s)	109, 229
MwoI	GCNNNNNNNGC	2 cut(s)	731, 839
NciI	CCSGG	1 cut(s)	455
NcoI	CCATGG	1 cut(s)	48
NdeII	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
NlaIV	GGNNCC	6 cut(s)	11, 146, 223, 274, 907, 1098
NmuCI	GTSAC	2 cut(s)	761, 947
NspI	RCATGY	3 cut(s)	607, 649, 892
OliI	CACNNNNGTG	1 cut(s)	175
PciI	ACATGT	1 cut(s)	603
PdmI	GAANNNNTTC	1 cut(s)	1203
PfeI	GAWTC	3 cut(s)	99, 567, 818
PinAI	ACCGGT	1 cut(s)	92
PkrI	GCNGC	7 cut(s)	372, 473, 544, 736, 880, 1126, 1158
PleI	GAGTC	1 cut(s)	614
PpsI	GAGTC	1 cut(s)	614
PscI	ACATGT	1 cut(s)	603
PsiI	TTATAA	1 cut(s)	81
Psp124BI	GAGCTC	1 cut(s)	984
Psp6I	CCWGG	2 cut(s)	107, 227
PspFI	CCCAGC	2 cut(s)	440, 559
PspGI	CCWGG	2 cut(s)	107, 227
PspN4I	GGNNCC	6 cut(s)	11, 146, 223, 274, 907, 1098
PstNI	CAGNNNCTG	1 cut(s)	902
PsuI	RGATCY	2 cut(s)	70, 1096
PvuII	CAGCTG	1 cut(s)	881
RsaI	GTAC	8 cut(s)	160, 319, 638, 644, 863, 930, 956, 1083
RsaNI	GTAC	8 cut(s)	159, 318, 637, 643, 862, 929, 955, 1082
RseI	CAYNNNNRTG	2 cut(s)	175, 789
SacI	GAGCTC	1 cut(s)	984
SalI	GTCGAC	1 cut(s)	1163
SaqAI	TTAA	3 cut(s)	189, 407, 552
SatI	GCNGC	7 cut(s)	371, 472, 543, 735, 879, 1125, 1157
Sau3AI	GATC	6 cut(s)	70, 243, 503, 529, 1096, 1212
ScaI	AGTACT	1 cut(s)	863
SchI	GAGTC	1 cut(s)	614
ScrFI	CCNGG	3 cut(s)	109, 229, 455
SduI	GDGCHC	2 cut(s)	855, 984
SfcI	CTRYAG	1 cut(s)	398
SmiMI	CAYNNNNRTG	2 cut(s)	175, 789
SmlI	CTYRAG	1 cut(s)	201
SmoI	CTYRAG	1 cut(s)	201
Sse9I	AATT	4 cut(s)	85, 549, 625, 720
SsiI	CCGC	5 cut(s)	45, 263, 471, 545, 1124
SspI	AATATT	2 cut(s)	331, 672
SspMI	CTAG	3 cut(s)	342, 497, 1085
SstI	GAGCTC	1 cut(s)	984
StyD4I	CCNGG	3 cut(s)	107, 227, 453
StyI	CCWWGG	2 cut(s)	48, 1119
TaaI	ACNGT	5 cut(s)	402, 761, 767, 933, 959
TaiI	ACGT	2 cut(s)	168, 954
TaqI	TCGA	4 cut(s)	375, 387, 608, 1164
TaqII	GACCGA	1 cut(s)	224
TasI	AATT	4 cut(s)	85, 549, 625, 720
TatI	WGTACW	4 cut(s)	317, 642, 861, 954
TauI	GCSGC	2 cut(s)	474, 1127
TfiI	GAWTC	3 cut(s)	99, 567, 818
Tru1I	TTAA	3 cut(s)	189, 407, 552
Tru9I	TTAA	3 cut(s)	189, 407, 552
TscAI	CASTG	7 cut(s)	25, 407, 707, 732, 946, 1008, 1095
TseFI	GTSAC	2 cut(s)	761, 947
TseI	GCWGC	5 cut(s)	370, 542, 734, 878, 1156
Tsp45I	GTSAC	2 cut(s)	761, 947
TspDTI	ATGAA	5 cut(s)	175, 857, 1022, 1029, 1062
TspGWI	ACGGA	2 cut(s)	51, 542
TspRI	CASTG	7 cut(s)	25, 407, 707, 732, 946, 1008, 1095
XceI	RCATGY	3 cut(s)	607, 649, 892
XcmI	CCANNNNNNNNNTGG	1 cut(s)	215
XmiI	GTMKAC	2 cut(s)	450, 1164
XmnI	GAANNNNTTC	1 cut(s)	1203
XspI	CTAG	3 cut(s)	342, 497, 1085
ZrmI	AGTACT	1 cut(s)	863

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.